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Nostoc flagelliforme is a terrestrial cyanobacterium that can resist many types of stressors, including drought, ultraviolet radiation, and extreme temperatures. In this study, we identified the drought tolerance gene NfcrtO, which encodes a β-carotene ketolase, through screening the transcriptome of N. flagelliforme under water loss stress. Prokaryotic expression of NfcrtO under 0.6 mol/L sorbitol or under 0.3 mol/L NaCl stress significantly increased the growth rate of Escherichia coli. When NfcrtO was heterologously expressed in rice, the seedling height and root length of NfcrtO-overexpressing rice plants were significantly higher than those of the wild type (WT) plants grown on ½ Murashige and Skoog solid medium with 120 mmol/L mannitol at the seedling stage. Transcriptome analysis revealed that NfcrtO was involved in osmotic stress, antioxidant, and other stress-related pathways. Additionally, the survival rate of the NfcrtO-overexpression lines was significantly higher than that of the WT line under both hydroponic stress (24% PEG and 100 mmol/L H2O2) and soil drought treatment at the seedling stage. Physiological traits, including the activity levels of superoxide dismutase, peroxidase, catalase, total antioxidant capacity, and the contents of proline, trehalose, and soluble sugar, were significantly improved in the NfcrtO-overexpression lines relative to those in the WT line under 20% PEG treatment. Furthermore, when water was withheld at the booting stage, the grain yield per plant of NfcrtO-overexpression lines was significantly higher than that of the WT line. Yeast two-hybrid analysis identified interactions between NfcrtO and Dna J protein, E3 ubiquitin-protein ligase, and pyrophosphate-energized vacuolar membrane proton pump. Thus, heterologous expression of NfcrtO in rice could significantly improve the tolerance of rice to osmotic stress, potentially facilitating the development of new rice varieties.

Based on self-determination theory, our research investigated the relationship between spiritual leadership and career satisfaction, and explored the roles of basic need satisfaction and power distance orientation in this relationship. Our research used a Chinese sample collected from 260 participants to verify the proposed theoretical model. The findings suggested that spiritual leadership had a positive relationship with career satisfaction, and basic need satisfaction played a mediator in this positive relationship. In addition, the indirect effect of spiritual leadership on career satisfaction through basic need satisfaction relied on subordinate power distance orientation, such that the indirect effect was stronger when power distance orientation was low than when it was high. Our research further provided several theoretical and practical implications for future research., Author(s): Ju Yang [sup.1] , Fu Yang [sup.1] , Ningning Gao [sup.1] Author Affiliations: (1) grid.443347.3, 0000 0004 1761 2353, School of Business Administration, Southwestern University of Finance and Economics, [...]

This paper mainly reviewed the study progress of CO2 separation membranes based on zeolite materials, including the CO2/CH4 separation performance of zeolite inorganic membranes with different topological structures and zeolite-filled mixed matrix membranes. The current research bottlenecks and corresponding improvement methods of the two membranes were discussed. According to the above points, the following two points were proposed as future research focus to promote the industrialization of zeolite- based CO2 separation membranes: preparation and defects-healing of ultra-thin inorganic membranes, structural regulation and structure-activity relationship research of mixed matrix membranes. [ABSTRACT FROM AUTHOR]

In this study, we successfully constructed a high‐performance fluorescent material for the detection of toxic pollutants in water, [Cd(2‐bpmh)(BDC)H2O]n (1) (2‐bpmh=1,4‐bis(2‐pyridyl)‐2,3‐diaza‐1,3‐butadiene, H2BDC=terephthalic acid), which achieved high yield under the condition of solvothermal reaction. Single crystal X‐ray analysis revealed that complex 1 forms a one‐dimensional chain structure, which is further assembled into a three‐dimensional structure through hydrogen bonding interactions. The luminescence sensing research showed that 1 not only displayed excellent luminescence quenching efficiency, but also could specifically recognize Cr2O72−, CrO42− and Fe3+ ions in water. [ABSTRACT FROM AUTHOR]

The preparation of an electroactive matrix for the immobilization of the bioprobe shows great promise to construct the label-free biosensors. Herein, the electroactive metal-organic coordination polymer has been in-situ prepared by pre-assembly of a layer of trithiocynate (TCY) on a gold electrode (AuE) through Au-S bond, followed by repetitive soaking in Cu(NO3)2 solution and TCY solutions. Then the gold nanoparticles (AuNPs) and the thiolated thrombin aptamers were successively assembled on the electrode surface, and thus the electrochemical electroactive aptasensing layer for thrombin was achieved. The preparation process of the biosensor was characterized by an atomic force microscope (AFM), attenuated total reflection-Fourier transform infrared (ATR-FTIR), and electrochemical methods. Electrochemical sensing assays showed that the formation of the aptamer-thrombin complex changed the microenvironment and the electro-conductivity of the electrode interface, causing the electrochemical signal suppression of the TCY-Cu2+ polymer. Additionally, the target thrombin can be label-free analyzed. Under optimal conditions, the aptasensor can detect thrombin in the concentration range from 1.0 fM to 1.0 μM, with a detection limit of 0.26 fM. The spiked recovery assay showed that the recovery of the thrombin in human serum samples was 97.2–103%, showing that the biosensor is feasible for biomolecule analysis in a complex sample. [ABSTRACT FROM AUTHOR]

One zinc‐based metal organic framework (MOF): [Zn(LBIX)(HEA)]n (1) was synthesized by hydrothermal synthesis with 4,4′‐bis((1H‐imidazol‐1‐yl)methyl)‐1,1′‐biphenyl (LBIX) and 1,2‐phenylenediacetic acid (H2HEA) as ligands. The structure was characterized by single‐crystal X‐ray diffraction analysis, elemental analysis, infrared spectroscopy and thermogravimetric analysis. The single crystal structure analysis shows that the complex extends along three directions of a, b and c, forming a three‐dimensional structure. Experiments related to fluorescence show that the complex 1 has good fluorescence performance in aqueous solution and can be utilized to quickly identify Fe3+, CrO42− and Cr2O72− ions (Ksv quenching efficiency for Fe3+:1.71×104 M−1, CrO42−:1.07×104 M−1 and Cr2O72−:1.15×104 M−1) and low detection limits (Fe3+:1.52 μM, CrO42−:1.53 μM and Cr2O72−:1.70 μM). [ABSTRACT FROM AUTHOR]