6 results on '"Coutinho Rodrigues, Caio Junior Balduino"'
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2. Ultrastructural and Cytotoxic Effects of Metarhizium robertsii Infection on Rhipicephalus microplus Hemocytes.
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Fiorotti, Jéssica, Menna-Barreto, Rubem Figueiredo Sadok, Gôlo, Patrícia Silva, Coutinho-Rodrigues, Caio Junior Balduino, Bitencourt, Ricardo Oliveira Barbosa, Spadacci-Morena, Diva Denelle, Angelo, Isabele da Costa, and Bittencourt, Vânia Rita Elias Pinheiro
- Subjects
METARHIZIUM ,RHIPICEPHALUS ,IMMUNE response ,ARTHROPODA physiology ,ELECTRON microscopy - Abstract
Metarhizium is an entomopathogenic fungus widely employed in the biological control of arthropods. Hemocytes present in the hemolymph of invertebrates are the cells involved in the immune response of arthropods. Despite this, knowledge about Rhipicephalus microplus hemocytes morphological aspects as well as their role in response to the fungal infection is scarce. The present study aimed to analyze the hemocytes of R. microplus females after Metarhizium robertsii infection, using light and electron microscopy approaches associated with the cytotoxicity evaluation. Five types of hemocytes (prohemocytes, spherulocytes, plasmatocytes, granulocytes, and oenocytoids) were described in the hemolymph of uninfected ticks, while only prohemocytes, granulocytes, and plasmatocytes were observed in fungus-infected tick females. Twenty-four hours after the fungal infection, only granulocytes and plasmatocytes were detected in the transmission electron microscopy analysis. Hemocytes from fungus-infected tick females showed several cytoplasmic vacuoles with different electron densities, and lipid droplets in close contact to low electron density vacuoles, as well as the formation of autophagosomes and subcellular material in different stages of degradation could also be observed. M. robertsii propagules were more toxic to tick hemocytes in the highest concentration tested (1.0 × 10
8 conidia mL−1 ). Interestingly, the lowest fungus concentration did not affect significantly the cell viability. Microanalysis showed that cells granules from fungus-infected and uninfected ticks had similar composition. This study addressed the first report of fungal cytotoxicity analyzing ultrastructural effects on hemocytes of R. microplus infected with entomopathogenic fungi. These results open new perspectives for the comprehension of ticks physiology and pathology, allowing the identification of new targets for the biological control. [ABSTRACT FROM AUTHOR]- Published
- 2019
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3. <italic>Rhipicephalus microplus</italic> infected by <italic>Metarhizium</italic>: unveiling hemocyte quantification, GFP-fungi virulence, and ovary infection.
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de Paulo, Jéssica Fiorotti, Camargo, Mariana Guedes, Coutinho-Rodrigues, Caio Junior Balduino, Marciano, Allan Felipe, de Freitas, Maria Clemente, da Silva, Emily Mesquita, Gôlo, Patrícia Silva, Bittencourt, Vânia Rita Elias Pinheiro, Morena, Diva Denelle Spadacci, and da Costa Angelo, Isabele
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RHIPICEPHALUS ,METARHIZIUM ,BLOOD cells ,ARTHROPODA ,VETERINARY medicine ,HYPOCREALES ,CLAVICIPITACEAE - Abstract
Hemocytes, cells present in the hemocoel, are involved in the immune response of arthropods challenged with entomopathogens. The present study established the best methodology for harvesting hemocytes from
Rhipicephalus microplus and evaluated the number of hemocytes in addition to histological analysis from ovaries of fungus-infected females and tested the virulence of GFP-fungi transformants. Different centrifugation protocols were tested, and the one in which presented fewer disrupted cells and higher cell recovery was applied for evaluating the effect ofMetarhizium spp. on hemocytes againstR. microplus . After processing, protocol number 1 (i.e., hemolymph samples were centrifuged at 500×g for 3 min at 4 °C) was considered more efficient, with two isolates used (Metarhizium robertsii ARSEF 2575 andMetarhizium anisopliae ARSEF 549), both wild types and GFP, to assess their virulence. In the biological assays, the GFP-fungi were as virulent as wild types, showing no significant differences. Subsequently, hemocyte quantifications were performed after inoculation, which exhibited notable changes in the number of hemocytes, reducing by approximately 80% in females previously treated withMetarhizium isolates in comparison to non-treated females. Complementarily, 48 h after inoculation, in which hemolymph could not be obtained, histological analysis showed the high competence of these fungi to colonize ovary from ticks. Here, for the first time, the best protocol (i.e., very low cell disruption and high cell recovery) forR. microplus hemocyte obtaining was established aiming to guide directions to other studies that involves cellular responses from ticks to fungi infection. [ABSTRACT FROM AUTHOR]- Published
- 2018
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4. Virulence, proteolytic and lipolytic activities of Brazilian Beauveria bassiana s.l. isolates (Hypocreales: Clavicipitaceae) to Rhipicephalus microplus ticks (Acari: Ixodidae).
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Coutinho Rodrigues, Caio Junior Balduino, Perinotto, Wendell Marcelo de Souza, Beys da Silva, Walter Orlando, Santi, Lucélia, Berger, Markus, Marciano, Allan Felipe, Sá, Fillipe Araújo de, Nogueira, Michel Ruan dos Santos, Quinelato, Simone, and Bittencourt, Vânia Rita Elias Pinheiro
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MICROBIAL virulence , *PROTEOLYTIC enzymes , *LIPOLYTIC enzymes , *BEAUVERIA bassiana , *TICKS , *RHIPICEPHALUS , *ANIMAL behavior - Abstract
Beauveria bassianas.l. is a cosmopolitan fungus used in the control of different species of arthropods. The current study explored the virulence for ticks, proteolytic and lipolytic activities of 10 BrazilianB. bassianas.l. isolates. For this purpose,Rhipicephalus microplusbiological parameters was evaluated after immersion of the engorged females in fungal suspension (108conidia mL−1) and the enzymatic activities were performed posteriorly the fungal growth in minimal medium. After the biological assays, five isolates changed all parameters analysed with highest efficacy of approximately 61% (CG 206) and 66% (CG 481). However, we observed that the most virulent isolates did not show the highest enzymatic activities. Interestingly, CG 500, considered an isolate of intermediate efficacy, demonstrated higher enzymatic activities than the other isolates in four of five analyses (total protease, Pr1, Pr2 and lipase;p < .05). Based on these data, it seems reasonable to suggest that these enzymatic activities should not be used as markers of virulence for these fungal isolates. It is noteworthy that complementary studies regarding chitinase analyses, gene expression, production of toxins and hydrophobicity of conidia can be used in the selection of pathogenic isolates. [ABSTRACT FROM PUBLISHER]
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- 2016
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5. Exposure to a sublethal menadione concentration modifies the mycelial secretome and conidial enzyme activities of Metarhizium anisopliae sensu lato and increases its virulence against Rhipicephalus microplus.
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Coutinho-Rodrigues, Caio Junior Balduino, Rosa, Rafael Lopes da, Freitas, Maria Clemente de, Fiorotti, Jéssica, Berger, Markus, Santi, Lucélia, Beys-da-Silva, Walter Orlando, Yates III, John R., and Bittencourt, Vânia Rita Elias Pinheiro
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METARHIZIUM anisopliae , *MENADIONE , *RHIPICEPHALUS , *ENZYMES , *CELL communication , *FUNGAL enzymes , *DIGESTIVE enzymes , *RICKETTSIAL diseases - Abstract
• M. anisopliae s.l. isolate was low tolerant to menadione (MND). • Half of MND LC 50 was used as a sublethal dose in the experiments. • Changes in the colony and conidial enzymatic activities were noticed. • Better efficacy against R. microplus with fungal LC 50 and LT 50 reductions. • Mycelial secretome reveals a specific increase in some Pr1 isoforms. Menadione (MND) is known to induce oxidative stress in fungal cells. Here, we explore how exposure to this molecule alters conidial enzyme activities, fungal efficacy against Rhipicephalus microplus, and mycelial secretion (secretome) of an isolate of Metarhizium anisopliae sensu lato. First, the fungus was exposed to different MND concentrations in potato-dextrose-agar (PDA) to determine the LC 50 by evaluating conidia germination (38μM). To ensure high cell integrity, a sublethal dose of MND (half of LC 50) was added to solid (PDA MND) and liquid media (MS MND). Changes in colony growth, a slight reduction in conidia production, decreases in conidial surface Pr1 and Pr2 activities as well as improvements in proteolytic and antioxidant (catalase, superoxide dismutase, and peroxidase) conidial intracellular activities were observed for PDA MND conidia. Additionally, PDA MND conidia had the best results for killing tick larvae, with the highest mortality rates until 15 days after treatment, which reduces both LC 50 and LT 50 , particularly at 108 conidia mL−1. The diversity of secreted proteins after growth in liquid medium + R. microplus cuticle (supplemented or not with half of MND LC 50), was evaluated by mass spectrometry-based proteomics. A total of 654 proteins were identified, 31 of which were differentially regulated (up or down) and mainly related to antioxidant activity (catalase), pathogenicity (Pr1B, Pr1D, and Pr1K), cell repair, and morphogenesis. In the exclusively MS MND profile, 48 proteins, mostly associated with cellular signaling, nutrition, and antioxidant functions, were distinguished. Finally, enzymatic assays were performed to validate some of these proteins. Overall, supplementation with MND in the solid medium made conidia more efficient at controlling R. microplus larvae, especially by increasing, inside the conidia, the activity of some infection-related enzymes. In the liquid medium (a consolidated study model that mimics some infection conditions), proteins were up- and/or exclusively-regulated in the presence of MND, which opens a spectrum of new targets for further study to improve biological control of ticks using Metarhizium species. [ABSTRACT FROM AUTHOR]
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- 2021
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6. How Dopamine Influences Survival and Cellular Immune Response of Rhipicephalus microplus Inoculated with Metarhizium anisopliae .
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Corrêa TA, Fiorotti J, Mesquita E, Meirelles LN, Camargo MG, Coutinho-Rodrigues CJB, Marciano AF, Bittencourt VREP, and Golo PS
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Dopamine (DA) is a biogenic monoamine reported to modulate insect hemocytes. Although the immune functions of DA are known in insects, there is a lack of knowledge of DA's role in the immune system of ticks. The use of Metarhizium anisopliae has been considered for tick control, driving studies on the immune response of these arthropods challenged with fungi. The present study evaluated the effect of DA on the cellular immune response and survival of Rhipicephalus microplus inoculated with M. anisopliae blastospores. Exogenous DA increased both ticks' survival 72 h after M. anisopliae inoculation and the number of circulating hemocytes compared to the control group, 24 h after the treatment. The phagocytic index of tick hemocytes challenged with M. anisopliae did not change upon injection of exogenous DA. Phenoloxidase activity in the hemolymph of ticks injected with DA and the fungus or exclusively with DA was higher than in untreated ticks or ticks inoculated with the fungus alone, 72 h after treatment. DA was detected in the hemocytes of fungus-treated and untreated ticks. Unveiling the cellular immune response in ticks challenged with entomopathogenic fungi is important to improve strategies for the biological control of these ectoparasites.
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- 2021
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