28 results on '"Arras G"'
Search Results
2. Integrated control of postharvest citrus decay and induction of phytoalexins by Debaryomyces hansenii
- Author
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Arras, G. and Arru, S.
- Published
- 1999
3. Influence of antagonist, host fruit and pathogen on the biological control of postharvest fungal diseases by yeasts
- Author
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Lima, G, Arru, S, De Curtis, F, and Arras, G
- Published
- 1999
- Full Text
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4. Response of « Murcott » mandarins to storage temperature
- Author
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Arras, G. and Usai, M.
- Published
- 1991
5. Inhibition of postharvest fungal pathogens by Bacillus subtilis strains isolated from citrus fruit
- Author
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Arras, G.
- Published
- 1993
6. Rind essential oils and juice metabolites of cv. Fortune tangerines under two cold-storage regimes
- Author
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Arras, G. and Usai, M.
- Published
- 1993
7. Attività fungistatica di alcuni olii essenziali nei confronti dei principali agenti di alterazioni post-raccolta dei frutti di agrumi
- Author
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ARRAS, G. and PICCI, V.
- Published
- 1984
8. Predictive Models for Carcinogenicity and Mutagenicity: Frameworks, State-of-the-Art, and Perspectives.
- Author
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BENFENATI, E., BENIGNI, R., DEMARINI, D.M., HELMA, C., KIRKLAND, D., MARTIN, T.M., MAZZATORTA, P., OUEDRAOGO-ARRAS, G., RICHARD, A.M., SCHILTER, B., SCHOONEN, W.G. E. J., SNYDER, R.D., and YANG, C.
- Subjects
CARCINOGENICITY ,MUTAGENICITY testing ,PREDICTIVE tests ,BIOLOGICAL assay ,NUCLEIC acids ,TOXICOLOGICAL interactions ,GENETIC toxicology ,CARCINOGENESIS ,PROSPECTING - Abstract
Mutagenicity and carcinogenicity are endpoints of major environmental and regulatory concern. These endpoints are also important targets for development of alternative methods for screening and prediction due to the large number of chemicals of potential concern and the tremendous cost (in time, money, animals) of rodent carcinogenicity bioassays. Both mutagenicity and carcinogenicity involve complex, cellular processes that are only partially understood. Advances in technologies and generation of new data will permit a much deeper understanding. In silico methods for predicting mutagenicity and rodent carcinogenicity based on chemical structural features, along with current mutagenicity and carcinogenicity data sets, have performed well for local prediction (i.e., within specific chemical classes), but are less successful for global prediction (i.e., for a broad range of chemicals). The predictivity of in silico methods can be improved by improving the quality of the data base and endpoints used for modelling. In particular, in vitro assays for clastogenicity need to be improved to reduce false positives (relative to rodent carcinogenicity) and to detect compounds that do not interact directly with DNA or have epigenetic activities. New assays emerging to complement or replace some of the standard assays include Vitotox™, GreenScreenGC, and RadarScreen. The needs of industry and regulators to assess thousands of compounds necessitate the development of high-throughput assays combined with innovative data-mining and in silico methods. Various initiatives in this regard have begun, including CAESAR, OSIRIS, CHEMOMENTUM, CHEMPREDICT, OpenTox, EPAA, and ToxCast™. In silico methods can be used for priority setting, mechanistic studies, and to estimate potency. Ultimately, such efforts should lead to improvements in application of in silico methods for predicting carcinogenicity to assist industry and regulators and to enhance protection of public health. [ABSTRACT FROM AUTHOR]
- Published
- 2009
- Full Text
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9. Paracrine signalling between intestinal epithelial and tumour cells induces a regenerative programme.
- Author
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Jacquemin G, Wurmser A, Huyghe M, Sun W, Homayed Z, Merle C, Perkins M, Qasrawi F, Richon S, Dingli F, Arras G, Loew D, Vignjevic D, Pannequin J, and Fre S
- Subjects
- Animals, Ecosystem, Epithelial Cells metabolism, Mice, Signal Transduction, Transcription Factors metabolism, Adaptor Proteins, Signal Transducing metabolism, Neoplasms
- Abstract
Tumours are complex ecosystems composed of different types of cells that communicate and influence each other. While the critical role of stromal cells in affecting tumour growth is well established, the impact of mutant cancer cells on healthy surrounding tissues remains poorly defined. Here, using mouse intestinal organoids, we uncover a paracrine mechanism by which intestinal cancer cells reactivate foetal and regenerative YAP-associated transcriptional programmes in neighbouring wildtype epithelial cells, rendering them adapted to thrive in the tumour context. We identify the glycoprotein thrombospondin-1 (THBS1) as the essential factor that mediates non-cell-autonomous morphological and transcriptional responses. Importantly, Thbs1 is associated with bad prognosis in several human cancers. This study reveals the THBS1-YAP axis as the mechanistic link mediating paracrine interactions between epithelial cells in intestinal tumours., Competing Interests: GJ, AW, MH, WS, ZH, CM, MP, FQ, SR, FD, GA, DL, DV, JP, SF No competing interests declared, (© 2022, Jacquemin et al.)
- Published
- 2022
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10. Reciprocal regulation of Aurora kinase A and ATIP3 in the control of metaphase spindle length.
- Author
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Nehlig A, Seiler C, Steblyanko Y, Dingli F, Arras G, Loew D, Welburn J, Prigent C, Barisic M, and Nahmias C
- Subjects
- HeLa Cells, Humans, Metaphase, Microtubule-Associated Proteins genetics, Microtubules genetics, Mitosis genetics, Aurora Kinase A genetics, Kinesins genetics, Phosphoproteins genetics, Spindle Apparatus genetics, Tumor Suppressor Proteins genetics
- Abstract
Maintaining the integrity of the mitotic spindle in metaphase is essential to ensure normal cell division. We show here that depletion of microtubule-associated protein ATIP3 reduces metaphase spindle length. Mass spectrometry analyses identified the microtubule minus-end depolymerizing kinesin Kif2A as an ATIP3 binding protein. We show that ATIP3 controls metaphase spindle length by interacting with Kif2A and its partner Dda3 in an Aurora kinase A-dependent manner. In the absence of ATIP3, Kif2A and Dda3 accumulate at spindle poles, which is consistent with reduced poleward microtubule flux and shortening of the spindle. ATIP3 silencing also limits Aurora A localization to the poles. Transfection of GFP-Aurora A, but not kinase-dead mutant, rescues the phenotype, indicating that ATIP3 maintains Aurora A activity on the poles to control Kif2A targeting and spindle size. Collectively, these data emphasize the pivotal role of Aurora kinase A and its mutual regulation with ATIP3 in controlling spindle length.
- Published
- 2021
- Full Text
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11. Trans-Synaptic Signaling through the Glutamate Receptor Delta-1 Mediates Inhibitory Synapse Formation in Cortical Pyramidal Neurons.
- Author
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Fossati M, Assendorp N, Gemin O, Colasse S, Dingli F, Arras G, Loew D, and Charrier C
- Subjects
- Animals, Cerebral Cortex physiology, Female, HEK293 Cells, Humans, Male, Mice, Signal Transduction physiology, Synaptic Transmission physiology, Neurogenesis physiology, Pyramidal Cells physiology, Receptors, Glutamate metabolism, Synapses physiology
- Abstract
Fine orchestration of excitatory and inhibitory synaptic development is required for normal brain function, and alterations may cause neurodevelopmental disorders. Using sparse molecular manipulations in intact brain circuits, we show that the glutamate receptor delta-1 (GluD1), a member of ionotropic glutamate receptors (iGluRs), is a postsynaptic organizer of inhibitory synapses in cortical pyramidal neurons. GluD1 is selectively required for the formation of inhibitory synapses and regulates GABAergic synaptic transmission accordingly. At inhibitory synapses, GluD1 interacts with cerebellin-4, an extracellular scaffolding protein secreted by somatostatin-expressing interneurons, which bridges postsynaptic GluD1 and presynaptic neurexins. When binding to its agonist glycine or D-serine, GluD1 elicits non-ionotropic postsynaptic signaling involving the guanine nucleotide exchange factor ARHGEF12 and the regulatory subunit of protein phosphatase 1 PPP1R12A. Thus, GluD1 defines a trans-synaptic interaction regulating postsynaptic signaling pathways for the proper establishment of cortical inhibitory connectivity and challenges the dichotomy between iGluRs and inhibitory synaptic molecules., (Copyright © 2019 The Authors. Published by Elsevier Inc. All rights reserved.)
- Published
- 2019
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12. Shigella promotes major alteration of gut epithelial physiology and tissue invasion by shutting off host intracellular transport.
- Author
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Ferrari ML, Malardé V, Grassart A, Salavessa L, Nigro G, Descorps-Declere S, Rohde JR, Schnupf P, Masson V, Arras G, Loew D, Sansonetti PJ, and Sauvonnet N
- Subjects
- Biological Transport, Caco-2 Cells, Cell Polarity, Colon metabolism, Colon microbiology, Colon pathology, Colon physiopathology, Dysentery, Bacillary metabolism, Dysentery, Bacillary pathology, Endocytosis, Humans, Intestinal Mucosa metabolism, Intestinal Mucosa pathology, Intestinal Mucosa physiology, Dysentery, Bacillary physiopathology, Intestinal Mucosa microbiology, Shigella flexneri
- Abstract
Intracellular trafficking pathways in eukaryotic cells are essential to maintain organelle identity and structure, and to regulate cell communication with its environment. Shigella flexneri invades and subverts the human colonic epithelium by the injection of virulence factors through a type 3 secretion system (T3SS). In this work, we report the multiple effects of two S. flexneri effectors, IpaJ and VirA, which target small GTPases of the Arf and Rab families, consequently inhibiting several intracellular trafficking pathways. IpaJ and VirA induce large-scale impairment of host protein secretion and block the recycling of surface receptors. Moreover, these two effectors decrease clathrin-dependent and -independent endocytosis. Therefore, S. flexneri infection induces a global blockage of host cell intracellular transport, affecting the exchange between cells and their external environment. The combined action of these effectors disorganizes the epithelial cell polarity, disturbs epithelial barrier integrity, promotes multiple invasion events, and enhances the pathogen capacity to penetrate into the colonic tissue in vivo., Competing Interests: The authors declare no conflict of interest.
- Published
- 2019
- Full Text
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13. Live Tracking of Inter-organ Communication by Endogenous Exosomes In Vivo.
- Author
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Verweij FJ, Revenu C, Arras G, Dingli F, Loew D, Pegtel DM, Follain G, Allio G, Goetz JG, Zimmermann P, Herbomel P, Del Bene F, Raposo G, and van Niel G
- Subjects
- Animals, Cells, Cultured, Proteomics methods, Zebrafish, Biological Transport physiology, Endothelial Cells metabolism, Exosomes metabolism, Extracellular Vesicles metabolism
- Abstract
Extracellular vesicles (EVs) are released by most cell types but providing evidence for their physiological relevance remains challenging due to a lack of appropriate model organisms. Here, we developed an in vivo model to study EV function by expressing CD63-pHluorin in zebrafish embryos. A combination of imaging methods and proteomic analysis allowed us to study biogenesis, composition, transfer, uptake, and fate of individual endogenous EVs. We identified a subpopulation of EVs with exosome features, released in a syntenin-dependent manner from the yolk syncytial layer into the blood circulation. These exosomes are captured, endocytosed, and degraded by patrolling macrophages and endothelial cells in the caudal vein plexus (CVP) in a scavenger receptor- and dynamin-dependent manner. Interference with exosome biogenesis affected CVP growth, suggesting a role in trophic support. Altogether, our work represents a system for studying endogenous EV function in vivo with high spatiotemporal accuracy, demonstrating functional inter-organ communication by exosomes., (Copyright © 2019 Elsevier Inc. All rights reserved.)
- Published
- 2019
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14. Aberrant ERBB4-SRC Signaling as a Hallmark of Group 4 Medulloblastoma Revealed by Integrative Phosphoproteomic Profiling.
- Author
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Forget A, Martignetti L, Puget S, Calzone L, Brabetz S, Picard D, Montagud A, Liva S, Sta A, Dingli F, Arras G, Rivera J, Loew D, Besnard A, Lacombe J, Pagès M, Varlet P, Dufour C, Yu H, Mercier AL, Indersie E, Chivet A, Leboucher S, Sieber L, Beccaria K, Gombert M, Meyer FD, Qin N, Bartl J, Chavez L, Okonechnikov K, Sharma T, Thatikonda V, Bourdeaut F, Pouponnot C, Ramaswamy V, Korshunov A, Borkhardt A, Reifenberger G, Poullet P, Taylor MD, Kool M, Pfister SM, Kawauchi D, Barillot E, Remke M, and Ayrault O
- Subjects
- Adolescent, Animals, Carcinogenesis pathology, Cell Line, Tumor, Cerebellar Neoplasms genetics, Cerebellum pathology, Child, Child, Preschool, Female, Gene Expression Profiling, Gene Expression Regulation, Neoplastic, Humans, Infant, Male, Medulloblastoma genetics, Mice, Mice, Transgenic, Phosphorylation, Proteome metabolism, Proteomics methods, Signal Transduction, src-Family Kinases genetics, Cerebellar Neoplasms pathology, Medulloblastoma pathology, Receptor, ErbB-4 metabolism, src-Family Kinases metabolism
- Abstract
The current consensus recognizes four main medulloblastoma subgroups (wingless, Sonic hedgehog, group 3 and group 4). While medulloblastoma subgroups have been characterized extensively at the (epi-)genomic and transcriptomic levels, the proteome and phosphoproteome landscape remain to be comprehensively elucidated. Using quantitative (phospho)-proteomics in primary human medulloblastomas, we unravel distinct posttranscriptional regulation leading to highly divergent oncogenic signaling and kinase activity profiles in groups 3 and 4 medulloblastomas. Specifically, proteomic and phosphoproteomic analyses identify aberrant ERBB4-SRC signaling in group 4. Hence, enforced expression of an activated SRC combined with p53 inactivation induces murine tumors that resemble group 4 medulloblastoma. Therefore, our integrative proteogenomics approach unveils an oncogenic pathway and potential therapeutic vulnerability in the most common medulloblastoma subgroup., (Copyright © 2018 Elsevier Inc. All rights reserved.)
- Published
- 2018
- Full Text
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15. Eml1 loss impairs apical progenitor spindle length and soma shape in the developing cerebral cortex.
- Author
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Bizzotto S, Uzquiano A, Dingli F, Ershov D, Houllier A, Arras G, Richards M, Loew D, Minc N, Croquelois A, Houdusse A, and Francis F
- Subjects
- Animals, Cells, Cultured, Cerebral Cortex metabolism, Classical Lissencephalies and Subcortical Band Heterotopias metabolism, Female, Mice, Mice, Knockout, Neural Stem Cells metabolism, Spindle Apparatus metabolism, Cerebral Cortex pathology, Classical Lissencephalies and Subcortical Band Heterotopias pathology, Microtubule-Associated Proteins physiology, Neural Stem Cells pathology, Spindle Apparatus pathology
- Abstract
The ventricular zone (VZ) of the developing cerebral cortex is a pseudostratified epithelium that contains progenitors undergoing precisely regulated divisions at its most apical side, the ventricular lining (VL). Mitotic perturbations can contribute to pathological mechanisms leading to cortical malformations. The HeCo mutant mouse exhibits subcortical band heterotopia (SBH), likely to be initiated by progenitor delamination from the VZ early during corticogenesis. The causes for this are however, currently unknown. Eml1, a microtubule (MT)-associated protein of the EMAP family, is impaired in these mice. We first show that MT dynamics are perturbed in mutant progenitor cells in vitro. These may influence interphase and mitotic MT mechanisms and indeed, centrosome and primary cilia were altered and spindles were found to be abnormally long in HeCo progenitors. Consistently, MT and spindle length regulators were identified in EML1 pulldowns from embryonic brain extracts. Finally, we found that mitotic cell shape is also abnormal in the mutant VZ. These previously unidentified VZ characteristics suggest altered cell constraints which may contribute to cell delamination.
- Published
- 2017
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16. Proteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes.
- Author
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Kowal J, Arras G, Colombo M, Jouve M, Morath JP, Primdal-Bengtson B, Dingli F, Loew D, Tkach M, and Théry C
- Subjects
- Biomarkers metabolism, Humans, Antigens, CD metabolism, Cell-Derived Microparticles metabolism, HSP70 Heat-Shock Proteins metabolism, Membrane Proteins metabolism, Proteomics
- Abstract
Extracellular vesicles (EVs) have become the focus of rising interest because of their numerous functions in physiology and pathology. Cells release heterogeneous vesicles of different sizes and intracellular origins, including small EVs formed inside endosomal compartments (i.e., exosomes) and EVs of various sizes budding from the plasma membrane. Specific markers for the analysis and isolation of different EV populations are missing, imposing important limitations to understanding EV functions. Here, EVs from human dendritic cells were first separated by their sedimentation speed, and then either by their behavior upon upward floatation into iodixanol gradients or by immuno-isolation. Extensive quantitative proteomic analysis allowing comparison of the isolated populations showed that several classically used exosome markers, like major histocompatibility complex, flotillin, and heat-shock 70-kDa proteins, are similarly present in all EVs. We identified proteins specifically enriched in small EVs, and define a set of five protein categories displaying different relative abundance in distinct EV populations. We demonstrate the presence of exosomal and nonexosomal subpopulations within small EVs, and propose their differential separation by immuno-isolation using either CD63, CD81, or CD9. Our work thus provides guidelines to define subtypes of EVs for future functional studies.
- Published
- 2016
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17. Moesin is a major regulator of centrosome behavior in epithelial cells with extra centrosomes.
- Author
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Sabino D, Gogendeau D, Gambarotto D, Nano M, Pennetier C, Dingli F, Arras G, Loew D, and Basto R
- Subjects
- Aneuploidy, Animals, Cell Death, Epithelial Cells cytology, Centrosome metabolism, Drosophila metabolism, Epithelial Cells metabolism, Microfilament Proteins metabolism, Spindle Apparatus metabolism, Up-Regulation
- Abstract
Centrosome amplification has severe consequences during development and is thought to contribute to a variety of diseases such as cancer and microcephaly. However, the adverse effects of centrosome amplification in epithelia are still not known. Here, we investigate the consequences of centrosome amplification in the Drosophila wing disc epithelium. We found that epithelial cells exhibit mechanisms of clustering but also inactivation of extra centrosomes. Importantly, these mechanisms are not fully efficient, and both aneuploidy and cell death can be detected. Epithelial cells with extra centrosomes generate tumors when transplanted into WT hosts and inhibition of cell death results in tissue over-growth and disorganization. Using SILAC-fly, we found that Moesin, a FERM domain protein, is specifically upregulated in wing discs with extra centrosomes. Moesin localizes to the centrosomes and mitotic spindle during mitosis, and we show that Moesin upregulation influences extra-centrosome behavior and robust bipolar spindle formation. This study provides a mechanistic explanation for the increased aneuploidy and transformation potential primed by centrosome amplification in epithelial tissues., (Copyright © 2015 The Authors. Published by Elsevier Ltd.. All rights reserved.)
- Published
- 2015
- Full Text
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18. Follow-up actions from positive results of in vitro genetic toxicity testing.
- Author
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Dearfield KL, Thybaud V, Cimino MC, Custer L, Czich A, Harvey JS, Hester S, Kim JH, Kirkland D, Levy DD, Lorge E, Moore MM, Ouédraogo-Arras G, Schuler M, Suter W, Sweder K, Tarlo K, van Benthem J, van Goethem F, and Witt KL
- Subjects
- Animals, Decision Support Techniques, Dose-Response Relationship, Drug, Endpoint Determination, Hazardous Substances standards, Humans, International Cooperation, Mutagenicity Tests trends, Mutagens standards, Risk Assessment, Hazardous Substances toxicity, Mutagenicity Tests methods, Mutagens toxicity
- Abstract
Appropriate follow-up actions and decisions are needed when evaluating and interpreting clear positive results obtained in the in vitro assays used in the initial genotoxicity screening battery (i.e., the battery of tests generally required by regulatory authorities) to assist in overall risk-based decision making concerning the potential effects of human exposure to the agent under test. Over the past few years, the International Life Sciences Institute (ILSI) Health and Environmental Sciences Institute (HESI) Project Committee on the Relevance and Follow-up of Positive Results in In Vitro Genetic Toxicity (IVGT) Testing developed a decision process flow chart to be applied in case of clear positive results in vitro. It provides for a variety of different possibilities and allows flexibility in choosing follow-up action(s), depending on the results obtained in the initial battery of assays and available information. The intent of the Review Subgroup was not to provide a prescriptive testing strategy, but rather to reinforce the concept of weighing the totality of the evidence. The Review Subgroup of the IVGT committee highlighted the importance of properly analyzing the existing data, and considering potential confounding factors (e.g., possible interactions with the test systems, presence of impurities, irrelevant metabolism), and chemical modes of action when analyzing and interpreting positive results in the in vitro genotoxicity assays and determining appropriate follow-up testing. The Review Subgroup also examined the characteristics, strengths, and limitations of each of the existing in vitro and in vivo genotoxicity assays to determine their usefulness in any follow-up testing., (Copyright © 2010 Wiley-Liss, Inc.)
- Published
- 2011
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19. International prevalidation studies of the EpiDerm 3D human reconstructed skin micronucleus (RSMN) assay: transferability and reproducibility.
- Author
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Aardema MJ, Barnett BC, Khambatta Z, Reisinger K, Ouedraogo-Arras G, Faquet B, Ginestet AC, Mun GC, Dahl EL, Hewitt NJ, Corvi R, and Curren RD
- Subjects
- Humans, Micronucleus Tests methods, Mutagens toxicity, Reproducibility of Results, Animal Testing Alternatives methods, Skin, Skin Irritancy Tests methods, Tissue Engineering
- Abstract
Recently, a novel in vitro reconstructed skin micronucleus (RSMN) assay incorporating the EpiDerm 3D human skin model (Curren et al., Mutat. Res. 607 (2006) 192-204; Mun et al., Mutat. Res. 673 (2009) 92-99) has been shown to produce comparable data when utilized in three different laboratories in the United States (Hu et al., Mutat. Res. 673 (2009) 100-108). As part of a project sponsored by the European cosmetics companies trade association (COLIPA), with a contribution from the European Center for the Validation of Alternative Methods (ECVAM), international prevalidation studies of the RSMN assay have been initiated. The assay was transferred and optimized in two laboratories in Europe, where dose-dependent, reproducibly positive results for mitomycin C and vinblastine sulfate were obtained. Further intra- and inter-laboratory reproducibility of the RSMN assay was established by testing three coded chemicals, N-ethyl-N-nitrosourea, cyclohexanone, and mitomycin C. All chemicals were correctly identified by all laboratories as either positive or negative. These results support the international inter-laboratory and inter-experimental reproducibility of the assay and reinforce the conclusion that the RSMN assay in the EpiDerm 3D human skin model is a valuable in vitro method for assessment of genotoxicity of dermally applied chemicals., (2010 Elsevier B.V. All rights reserved.)
- Published
- 2010
- Full Text
- View/download PDF
20. Synergic interactions between 2-deoxy-D-glucose and Candida saitoana enhances citrus green mould control.
- Author
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Arras G, Pani G, Molinu MG, Dore A, Venditti T, Petretto A, Marceddu S, and D'Hallewin G
- Subjects
- Agriculture methods, Antibiosis, Candida physiology, Citrus microbiology, Deoxyglucose pharmacology, Fungicides, Industrial pharmacology, Penicillium drug effects, Penicillium physiology, Plant Diseases microbiology
- Abstract
The activity of 2-deoxy-D-gLucose (2-DG) alone or in combination with a biocontrol yeast (Candida saitoana, strain 8C) was evaluated in vitro and in vivo against citrus green mould (Penicillium digitatum Sacc.). The in vitro assays were performed on amended potato dextrose agar (PDA) containing 0, 1.5, 3.0, 6.0, 15.0, 30.0 or 60.0 mM of 2-DG. P. digitatum conidia were sown on the amended media and growth inhibition occurred starting from 6.0 mM. A nearly total inhibition of the growth and spore germination occurred with 60.0 mM of 2-DG. The antagonist was not affected by any of the 2-DG concentrations employed and the amended plates resulted well colonized within 2 d post-treatment. In vivo assays were carried out with 'Hamlin' oranges, inoculated with P. digitatum 24 h before treating with: the antagonist; the above reported concentrations of 2-DG, or by combining the two treatments. Seven days post-treatment the inhibition activity exerted by 3.0, 6.0, 15.0, 30.0 and 60.0 mM of 2-DG combined with the yeast was 15, 37, 42, 63 and 84%, respectively. While that exerted by the antagonist was 22% and that by the different concentrations of 2-DG were 7, 11, 27, 42 and 57%, respectively. Compared to single treatments, the co-application significantly and in a synergic mode improved the control of decay. Alterations to the hyphae were observed by SEM when the pathogen was cultured on amended media and into the wounds of inoculated oranges.
- Published
- 2010
21. Host-pathogen-biocontrol agent interaction as affected by sequential application of Na2CO3 and CaCl2.
- Author
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Molinu GM, Arras G, Dore A, Venditti T, Petretto A, and D'Hallewin G
- Subjects
- Citrus microbiology, Culture Media, Food Packaging methods, Fungicides, Industrial adverse effects, Fungicides, Industrial pharmacology, Host-Pathogen Interactions drug effects, Immunity, Innate, Pest Control, Biological methods, Pichia growth & development, Pichia immunology, Calcium Chloride pharmacology, Carbonates pharmacology, Host-Pathogen Interactions physiology, Pichia drug effects
- Abstract
Among the alternatives to synthetic postharvest fungicides encouraging results have been reported with biocontrol agents, and on Citrus fruits, their efficacy was improved when co-applied with GRAS compounds or with physical means. Still, the reason for this increased efficacy has not been explained and therefore a study was performed using orange fruit (Citrus sinensis Osbec. cv 'Washington navel') as host, P. digitatum as the pathogen, a yeast (Pichia guiliermondii, isolate 5A) as the biocontrol agent, white 2% Na2CO3 (SC) and 1% CaCl2 were employed as GRAS compounds. When treatments were combined salts were applied sequentially, and SC preceded CaCl2 followed by the yeast. As a result of large scale trait with inoculated and un-inoculated fruit a clear beneficial interaction occurred when treatments were combined. SC exerted a direct fungistatic activity and an indirect one by inducing scoparone in host tissue. Also the isolate A5 induced the phytoalexin accumulation and when combined with SC a greater accumulation occurred within the first 7 days post-treatment. The application of CaCl2 alone had no effect on pathogenesis, while when combined with SC or with the yeast, decay was towered. The yeast growth on an amended medium was negatively affected by the addition of SC; while in vivo this effect was missing. The antagonist growth in vivo was enhanced when applied together with 1% CaCl2 also when applied with SC. The results reported improve our knowledge on the complex interactions among host, pathogen and the antagonist as affected by SC and CaCl2.
- Published
- 2009
22. A microarray to measure repair of damaged plasmids by cell lysates.
- Author
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Millau JF, Raffin AL, Caillat S, Claudet C, Arras G, Ugolin N, Douki T, Ravanat JL, Breton J, Oddos T, Dumontet C, Sarasin A, Chevillard S, Favier A, and Sauvaigo S
- Subjects
- Cell Line, Transformed, HeLa Cells, Humans, Spectrometry, Fluorescence, DNA Repair, Plasmids
- Abstract
DNA repair mechanisms constitute major defences against agents that cause cancer, degenerative disease and aging. Different repair systems cooperate to maintain the integrity of genetic information. Investigations of DNA repair involvement in human pathology require an efficient tool that takes into account the variety and complexity of repair systems. We have developed a highly sensitive damaged plasmid microarray to quantify cell lysate excision/synthesis (ES) capacities using small amounts of proteins. This microsystem is based on efficient immobilization and conservation on hydrogel coated glass slides of plasmid DNA damaged with a panel of genotoxic agents. Fluorescent signals are generated from incorporation of labelled dNTPs by DNA excision-repair synthesis mechanisms at plasmid sites. Highly precise DNA repair phenotypes i.e. simultaneous quantitative measures of ES capacities toward seven lesions repaired by distinct repair pathways, are obtained. Applied to the characterization of xeroderma pigmentosum (XP) cells at basal level and in response to a low dose of UVB irradiation, the assay showed the multifunctional role of different XP proteins in cell protection against all types of damage. On the other hand, measurement of the ES of peripheral blood mononuclear cells from six donors revealed significant diversity between individuals. Our results illustrate the power of such a parallelized approach with high potential for several applications including the discovery of new cancer biomarkers and the screening of chemical agents modulating DNA repair systems.
- Published
- 2008
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23. Induction of phytoalexins biosynthesis in orange fruit by the biocontrol yeast Rhodotorula glutinis.
- Author
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Arras G, D'Hallewin G, Molinu MG, Dore A, Venditti T, Fois M, Lima G, and Agabbio M
- Subjects
- Fruit, Penicillium drug effects, Plant Diseases microbiology, Rhodotorula growth & development, Sesquiterpenes, Phytoalexins, Citrus microbiology, Citrus physiology, Rhodotorula physiology, Terpenes metabolism
- Abstract
The biocontrol yeast Rhodotorula glutinis, isolate 21A, obtained from tomato fruit was used to control Penicillium digitatum, P. italicum and Botrytis cinerea on artificially wounded citrus fruit. Orange and satsuma mandarin fruit were treated with the biocontrol yeast, inoculated with the pathogens and stored for 7 days at 23 degrees C. On orange fruit the antagonist compared to the control reduced decay by 92.2, 88.4 and 96.2% for P. digitatum, P. italicum and B. cinerea, respectively. On satsuma mandarin fruit the same pathogens were inhibited by 96.2, 91.2 and 90.0%, respectively. Scanning electron microscope observations, focusing on the antagonist-pathogen interactions, revealed a fast colonization of the growing fungal mycelia by the yeast cells, but no sign of lytic activity on hyphae was observed. Moreover, the fruit accumulated the phytoalexins scoparone and scopoletin into artificial wounds previously treated by the yeast and either inoculated or uninoculated with the pathogen. The concentration of scoparone, which showed higher accumulation in fruit tissues, varied significantly in relation to the time lag between the application of the antagonist and the inoculation with the pathogen. In particular, the concentration of scoparone 4 days after application of the sole yeast was 69.0 microg x g(-1) fresh weight (FW), 6.3 times higher than in the uninoculated wounded tissues (11.0 microg x g(-1) FW). The phytoalexin accumulation was low (13.0 microg x g(-1)FW) applying the yeast jointly with P. digitatum into wounds, while it increased consistently (74.0 microg x g(-1)FW) when the antagonist was applied 24 h before the pathogen.
- Published
- 2006
24. Inhibitory activity of 2-deoxy-D-glucose and Candida saitoana against Penicillium digitatum.
- Author
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Arras G, Molinu MG, Dore A, Venditti T, Fois M, Petretto A, and D'Hallewin G
- Subjects
- Candida growth & development, Candida ultrastructure, Citrus drug effects, Citrus microbiology, Fungicides, Industrial pharmacology, Microscopy, Electron, Scanning, Penicillium ultrastructure, Candida physiology, Deoxyglucose pharmacology, Penicillium drug effects, Penicillium growth & development
- Abstract
The toxic activity of 2-deoxy-D-glucose (2-DG) alone or combined with the biocontrol yeast Candida saitoana strain 8C was evaluated in vitro and in vivo against the postharvest fungal pathogen Penicillium digitatum. In order to assess the effect of the 2-DG on both the biocontrol yeast and fungal pathogen, in vitro tests were performed in Petri dishes containing potato dextrose agar amended with different concentrations (1.5, 3.0, 6.0, 15.0, 30.0, 60.0 mM) of the sugar. The plates were then seeded with 25 microl of a P. digitatum conidial suspension at 10(5) conidia/mL. Result of the assays showed an enhanced inhibitory activity as concentration increased from 15.0 to 60.0 mM. Corroborated by SEM observations showing a reduced growth and the appearance of damaged hyphae were found. At 60 mM of 2-DG, a total inhibition occurred while concentrations from 1.5 to 6.0 mM resulted ineffective. The same tests evidenced no adverse effects on the yeast 8C at all tested concentrations. In vivo assays were carried out on orange fruit cv 'Biondo comune', wounded in 5 sites around the calyx. Each wound (2.5 wide and 3.4 mm depth) was first filled with 25 microl of a 0, 3.0, 6.0, 15.0, 30.0 or 60.0 mM 2-DG-water solution alone or combined with the yeast 8C at 10(8) cells/mL and then a 25 microl of the P. digitatum conidial suspension was added. Each treatment consisted of 3 replicates of 8 fruit (5 wounds/fruit) for a total 120 wounds per treatment. Oranges were maintained at 20 degrees C and high RH (95-98%) for up to 5 days, during which infection was monitored and the inhibitory activity calculated. The tests in vitro evedenced a significant slowing of the pathogen growth with the highest concentrations of 2-DG (15.0, 30.0 and 60.0 mM) with respect to the control; while at lower concentrations (1.5, 3.0, 6.0 mM) the development of the fungi was not significantly reduced. C. saitoana was resistant to all the doses employed to the abovementioned compound. In vivo the yeast alone was more effective compared to the sugar alone up to 6.0 mM while, at higher concentrations an additive effect was founded.
- Published
- 2006
25. Biological and physical approaches to improve induced resistance against green mold of stored citrus fruit.
- Author
-
Arras G, Dhallewin G, Petretto A, Marceddu S, Loche M, and Agabbio M
- Subjects
- Chromatography, High Pressure Liquid methods, Pest Control, Biological methods, Plant Diseases microbiology, Sesquiterpenes, Terpenes, Time Factors, Phytoalexins, Citrus metabolism, Citrus microbiology, Food Preservation methods, Penicillium growth & development, Plant Extracts analysis, Yeasts physiology
- Abstract
Health and environmental concerns have point out the need to improve or change several manufacturing steps in the food chain. In this context particular attention should be given to the technologies involved in fruits and vegetables production. Nearly all fresh fruit and vegetables are subjected to different periods of storage and/or shelf-life before of their consumption. This implies the need to protect the commodities from microbial spoilage. Some Citrus species (e.g. lemon and grapefruit) may be stored for several months before consumption and then post-harvest treatments are essential to contain green (Penicillium digitatum) and blue (P. italicum) moulds. Alternative approaches to chemicals usually have a lower efficacy in containing rots but fulfill the consumer's expectation. Among the alternative strategies, the improvement of host natural resistance is promising. In this regard, we report some results concerning the use of biotic (yeast) and abiotic agents as inducers of phytoalexin (i.e. scoparone and/or scopoletin) accumulation in Citrus rind and its importance in the control of fungal decay. In all experiments the inducers were applied on fruits before or 24 h after inoculation with P. digitatum and the rot severity was monitored 7 days later. The accumulation of phytoalexins was monitored according to a standard methodology by HPLC. In all experiments a positive correlation was found between increase of the phytoalexin scoparone in host tissue and reduction of decay.
- Published
- 2005
26. Fungitoxic activity of 12 essential oils against four postharvest citrus pathogens: chemical analysis of thymus capitatus oil and its effect in subatmospheric pressure conditions.
- Author
-
Arras G and Usai M
- Subjects
- Alternaria growth & development, Alternaria ultrastructure, Botrytis growth & development, Botrytis ultrastructure, Microbial Sensitivity Tests, Microscopy, Electron, Scanning, Oils, Volatile, Penicillium growth & development, Penicillium ultrastructure, Plants, Medicinal, Pressure, Vacuum, Alternaria drug effects, Antifungal Agents pharmacology, Botrytis drug effects, Citrus microbiology, Lamiaceae chemistry, Penicillium drug effects
- Abstract
The fungitoxic activity against Penicillium digitatum, Penicillium italicum, Botrytis cinerea, and Alternaria citri of 12 essential oils (EOs) distilled from medicinal plants is reported. The results of the in vitro trials show strong fungitoxic activity of Thymus capitatus (L.) Hofmgg EOs, which inhibited the growth of the four fungi at a concentration of 250 ppm (vol/vol). The other 11 essences reduced the development of the fungi from 95 to 9% at 250 ppm (vol/vol). The fungitoxic activity of T. capitatus EOs (75, 150, and 250 ppm) on healthy orange fruits, inoculated with P. digitatum (10(8) conidia ml(-1)) by spraying and placed in 10-liter desiccators, was weak at atmospheric pressure (3 to 10% inhibition at all three concentrations), while in vacuum conditions (0.5 bar), conidial mortality on the exocarp was high (90 to 97% at all three concentrations). These data proved not to be statistically different from treatments with thiabendazole-TBZ (2,000 ppm). Scanning electron microscope observations showed that T. capitatus EO vapors altered the morphology of P. digitatum hyphae and conidia. The gas-chromatographic analyses of thyme EO indicated that carvacrol was present at 81 to 83%, p-cymene at 4.5 to 5%, gamma-terpinene at 2.6 to 3.3%, caryophyllene at 1.5 to 1.6%, beta-myrcene at 1.6%, and linalool at 1.1 to 1.2%. Carvacrol proved to be the most important fungitoxic compound among the thyme EO constituents, but, unlike thyme EO, it caused alterations to the fruit at the concentration of 75 ppm.
- Published
- 2001
- Full Text
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27. Prolactin release induced by physical exercise is independent from peripheral vasoactive intestinal polypeptide secretion.
- Author
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Rolandi E, Reggiani E, Franceschini R, Arras GB, Cataldi A, de Lucia F, and Barreca T
- Subjects
- Adult, Humans, Male, Prolactin blood, Vasoactive Intestinal Peptide blood, Exercise, Prolactin metabolism, Vasoactive Intestinal Peptide metabolism
- Abstract
The plasma concentrations of vasoactive intestinal polypeptide and prolactin were measured before and after an exhaustive and a submaximal exercise test in 7 male marathon runners. A significant increase of vasoactive intestinal polypeptide was recorded after both tests, whereas the prolactin increase was observed only after the exhaustive exercise test. No significant correlation was found between the plasma vasoactive intestinal polypeptide and the plasma prolactin values recorded during the two exercise tests. Data suggest that the exercise-induced prolactin release occurs independently from variations of the vasoactive intestinal polypeptide levels in peripheral circulation.
- Published
- 1988
28. Nutritional status and body composition of adolescent female gymnasts.
- Author
-
Reggiani E, Arras GB, Trabacca S, Senarega D, and Chiodini G
- Subjects
- Body Mass Index, Child, Female, Humans, Body Composition physiology, Gymnastics, Nutritional Status physiology
- Abstract
Nutritional status and body composition of 26 young female gymnasts (average age 12 years) were studied. The body fat percentage (15% of total body weight) is low, still consistent with an excellent health. The caloric intake is lower (1552 kcal/day) than that recommended for their age group, however it is still within the standard by body weight (43 kcal/kg). They show and insufficient assumption of carbohydrates (47.7%), mineral salts and vitamins, so that a more detailed alimentary information is required in order to avoid undesired consequences on sport performance and growth rate.
- Published
- 1989
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