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Shigella promotes major alteration of gut epithelial physiology and tissue invasion by shutting off host intracellular transport.
- Source :
-
Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2019 Jul 02; Vol. 116 (27), pp. 13582-13591. Date of Electronic Publication: 2019 Jun 17. - Publication Year :
- 2019
-
Abstract
- Intracellular trafficking pathways in eukaryotic cells are essential to maintain organelle identity and structure, and to regulate cell communication with its environment. Shigella flexneri invades and subverts the human colonic epithelium by the injection of virulence factors through a type 3 secretion system (T3SS). In this work, we report the multiple effects of two S. flexneri effectors, IpaJ and VirA, which target small GTPases of the Arf and Rab families, consequently inhibiting several intracellular trafficking pathways. IpaJ and VirA induce large-scale impairment of host protein secretion and block the recycling of surface receptors. Moreover, these two effectors decrease clathrin-dependent and -independent endocytosis. Therefore, S. flexneri infection induces a global blockage of host cell intracellular transport, affecting the exchange between cells and their external environment. The combined action of these effectors disorganizes the epithelial cell polarity, disturbs epithelial barrier integrity, promotes multiple invasion events, and enhances the pathogen capacity to penetrate into the colonic tissue in vivo.<br />Competing Interests: The authors declare no conflict of interest.
- Subjects :
- Biological Transport
Caco-2 Cells
Cell Polarity
Colon metabolism
Colon microbiology
Colon pathology
Colon physiopathology
Dysentery, Bacillary metabolism
Dysentery, Bacillary pathology
Endocytosis
Humans
Intestinal Mucosa metabolism
Intestinal Mucosa pathology
Intestinal Mucosa physiology
Dysentery, Bacillary physiopathology
Intestinal Mucosa microbiology
Shigella flexneri
Subjects
Details
- Language :
- English
- ISSN :
- 1091-6490
- Volume :
- 116
- Issue :
- 27
- Database :
- MEDLINE
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Publication Type :
- Academic Journal
- Accession number :
- 31209035
- Full Text :
- https://doi.org/10.1073/pnas.1902922116