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1. Quantification of methanogen cell density in anaerobic granular sludge consortia by fluorescence in-situ hybridization.

Author

Tagawa, T., Syutsubo, K., Sekiguchil, Y., Ohashi, A., and Harada, H.

Subjects
*ARCHAEBACTERIA, *METHANOBACTERIACEAE, *MANURE gases, *OLIGONUCLEOTIDES, *UPFLOW anaerobic sludge blanket (UASB) reactor, *INDUSTRIAL wastes, *SEWAGE sludge
Abstract

Whole cell fluorescence in-situ hybridization (FISH) with 16S rRNA targeted oligonucleotides was applied to reveal the microbial ecological structure of UASB-grown granular sludge. The FISH analysis indicated that the members of the domain Archaea accounted for 28 to 53% of the total cells in various granular sludge sources, while Methanosaeta and Methanobacteriaceae cells accounted for 13 to 38%, and 4 to 27%, respectively. Methanosaeta cell density and Methanobacteriaceae cell density were strongly correlated, respectively, with acetate-utilizing methane production activity and with hydrogen-utilizing methane production activity. [ABSTRACT FROM AUTHOR]

Published
2000
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