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Quantification of methanogen cell density in anaerobic granular sludge consortia by fluorescence in-situ hybridization.
- Source :
-
Water Science & Technology . 2000, Vol. 42 Issue 3/4, p77-82. 6p. - Publication Year :
- 2000
-
Abstract
- Whole cell fluorescence in-situ hybridization (FISH) with 16S rRNA targeted oligonucleotides was applied to reveal the microbial ecological structure of UASB-grown granular sludge. The FISH analysis indicated that the members of the domain Archaea accounted for 28 to 53% of the total cells in various granular sludge sources, while Methanosaeta and Methanobacteriaceae cells accounted for 13 to 38%, and 4 to 27%, respectively. Methanosaeta cell density and Methanobacteriaceae cell density were strongly correlated, respectively, with acetate-utilizing methane production activity and with hydrogen-utilizing methane production activity. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 02731223
- Volume :
- 42
- Issue :
- 3/4
- Database :
- Academic Search Index
- Journal :
- Water Science & Technology
- Publication Type :
- Academic Journal
- Accession number :
- 26878069
- Full Text :
- https://doi.org/10.2166/wst.2000.0361