16 results on '"Zuo, Xiaolei"'
Search Results
2. Proximity labeling-assisted click conjugation for electrochemical analysis of specific subpopulations in circulating extracellular vesicles
- Author
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Cao, Yue, Zhou, Liang, Zhou, Guozhang, Liu, Wensheng, Cui, Haiyan, Cao, Ya, Zuo, Xiaolei, and Zhao, Jing
- Published
- 2024
- Full Text
- View/download PDF
3. Tetrahedral DNA frameworks for biosensing and imaging analysis in living cells
- Author
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Qi, Zhijie, Wei, Chen, Zhang, Fei, Wang, Zejun, and Zuo, Xiaolei
- Published
- 2024
- Full Text
- View/download PDF
4. Concept and Development of Algebraic Topological Framework Nucleic Acids.
- Author
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Song, Lu, Zuo, Xiaolei, and Li, Min
- Abstract
Nucleic acids are considered as promising materials for developing exquisite nanostructures from one to three dimensions. The advances of DNA nanotechnology facilitate ingenious design of DNA nanostructures with diverse shapes and sizes. Especially, the algebraic topological framework nucleic acids (ATFNAs) are functional DNA nanostructures that engineer guest molecules (e. g., nucleic acids, proteins, small molecules, and nanoparticles) stoichiometrically and spatially. The intrinsic precise properties and tailorable functionalities of ATFNAs hold great promise for biological applications, such as cell recognition and immunotherapy. This Perspective highlights the concept and development of precisely assembled ATFNAs, and outlines the new frontiers and opportunities for exploiting the structural advantages of ATFNAs for biological applications. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
5. Construction of Double‐enzyme Complexes with DNA Framework Nanorulers for Improving Enzyme Cascade Catalytic Efficiency.
- Author
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Cao, Nan, Wang, Shaopeng, Li, Fan, Mao, Xiuhai, Zuo, Xiaolei, Zhang, Yueyue, and Li, Min
- Subjects
POLYACRYLAMIDE gel electrophoresis ,ATOMIC force microscopy ,ENZYMES ,MULTIENZYME complexes ,NUCLEIC acids - Abstract
Efficient biocatalytic cascade reactions play a crucial role in guiding intricate, specific and selective intracellular transformation processes. However, the catalytic activity of the enzyme cascade reaction in bulk solution was greatly impacted by the spatial morphology and inter‐enzyme distance. The programmability and addressability nature of framework nucleic acid (FNA) allows to be used as scaffold for immobilization and to direct the spatial arrangement of enzyme cascade molecules. Here, we used tetrahedral DNA framework (TDF) as nanorulers to assemble two enzymes for constructing a double‐enzyme complex, which significantly enhance the catalytic efficiency of sarcosine oxidase (SOx)/horseradish peroxidase (HRP) cascade system. We synthesized four types of TDF nanorulers capable of programming the lateral distance between enzymes from 5.67 nm to 12.33 nm. Enzymes were chemical modified by ssDNA while preserving most catalytic activity. Polyacrylamide gel electrophoresis (PAGE), transmission electron microscopy (TEM) and atomic force microscopy (AFM) were used to verify the formation of double‐enzyme complex. Four types of double‐enzyme complexes with different enzyme distance were constructed, in which TDF26(SOx+HRP) exhibited the highest relative enzyme cascade catalytic activity, ~3.11‐fold of free‐state enzyme. Importantly, all the double‐enzyme complexes demonstrate a substantial improvement in enzyme cascade catalytic activity compared to free enzymes. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
6. High-throughput DNA synthesis for data storage.
- Author
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Yu, Meng, Tang, Xiaohui, Li, Zhenhua, Wang, Weidong, Wang, Shaopeng, Li, Min, Yu, Qiuliyang, Xie, Sijia, Zuo, Xiaolei, and Chen, Chang
- Subjects
DATA warehousing ,DNA ,DIGITAL technology ,DNA sequencing ,DNA synthesis - Abstract
With the explosion of digital world, the dramatically increasing data volume is expected to reach 175 ZB (1 ZB = 10
12 GB) in 2025. Storing such huge global data would consume tons of resources. Fortunately, it has been found that the deoxyribonucleic acid (DNA) molecule is the most compact and durable information storage medium in the world so far. Its high coding density and long-term preservation properties make itself one of the best data storage carriers for the future. High-throughput DNA synthesis is a key technology for "DNA data storage", which encodes binary data stream (0/1) into quaternary long DNA sequences consisting of four bases (A/G/C/T). In this review, the workflow of DNA data storage and the basic methods of artificial DNA synthesis technology are outlined first. Then, the technical characteristics of different synthesis methods and the state-of-the-art of representative commercial companies, with a primary focus on silicon chip microarray-based synthesis and novel enzymatic DNA synthesis are presented. Finally, the recent status of DNA storage and new opportunities for future development in the field of high-throughput, large-scale DNA synthesis technology are summarized. [ABSTRACT FROM AUTHOR]- Published
- 2024
- Full Text
- View/download PDF
7. Directing the Encapsulation of Single Cells with DNA Framework Nucleator‐Based Hydrogel Growth.
- Author
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Wei, Yuhan, Feng, Yueyue, Wang, Kaizhe, Wei, Yuhui, Li, Qian, Zuo, Xiaolei, Li, Bin, Li, Jiang, Wang, Lihua, Fan, Chunhai, and Zhu, Ying
- Subjects
DNA structure ,DNA ,BIOMIMETIC materials ,CELL culture ,STRUCTURAL frames ,SINGLE-stranded DNA - Abstract
Encapsulating individual mammalian cells with biomimetic materials holds potential in ex vivo cell culture and engineering. However, current methodologies often present tradeoffs between homogeneity, stability, and cell compatibility. Here, inspired by bacteria that use proteins stably anchored on their outer membranes to nucleate biofilm growth, we develop a single‐cell encapsulation strategy by using a DNA framework structure as a nucleator (DFN) to initiate the growth of DNA hydrogels under cell‐friendly conditions. We find that among the tested structures, the tetrahedral DFN can evenly and stably reside on cell membranes, effectively initiating hybridization chain reactions which generate homogeneously dense yet flexible single‐cell encapsulation for diverse cell lines. The encapsulation persists for up to 72 hours in a serum‐containing cell culture environment, representing a ~70‐fold improvement compared to encapsulations mediated by single‐stranded DNA nucleators. The metabolism and proliferation of the encapsulated cells are suppressed, but can be restored to the original efficiencies upon release, suggesting the superior cell compatibility of the encapsulation. We also find that compared to naked cells, the encapsulated cells exhibit a lower autophagy level after undergoing mechanical stress, suggesting the protective effect of the DNA encapsulation. This method may provide a new tool for ex vivo cell engineering. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
8. DNA Framework–Programmed Nanoscale Enzyme Assemblies.
- Author
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Cao, Nan, Guo, Ruiyan, Song, Ping, Wang, Shaopeng, Liu, Gang, Shi, Jiye, Wang, Lihua, Li, Min, Zuo, Xiaolei, Yang, Xiurong, Fan, Chunhai, Li, Mingqiang, and Zhang, Yueyue
- Published
- 2024
- Full Text
- View/download PDF
9. A Programmable DNAzyme for the Sensitive Detection of Nucleic Acids.
- Author
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Shi, Chenzhi, Yang, Donglei, Ma, Xiaowei, Pan, Li, Shao, Yuanchuan, Arya, Gaurav, Ke, Yonggang, Zhang, Chuan, Wang, Fuan, Zuo, Xiaolei, Li, Min, and Wang, Pengfei
- Subjects
DEOXYRIBOZYMES ,NUCLEIC acids ,PROSTATE cancer ,MOLECULAR diagnosis ,STOMACH cancer ,POINT-of-care testing - Abstract
Nucleic acids in biofluids are emerging biomarkers for the molecular diagnostics of diseases, but their clinical use has been hindered by the lack of sensitive detection assays. Herein, we report the development of a sensitive nucleic acid detection assay named SPOT (sensitive loop‐initiated DNAzyme biosensor for nucleic acid detection) by rationally designing a catalytic DNAzyme of endonuclease capability into a unified one‐stranded allosteric biosensor. SPOT is activated once a nucleic acid target of a specific sequence binds to its allosteric module to enable continuous cleavage of molecular reporters. SPOT provides a highly robust platform for sensitive, convenient and cost‐effective detection of low‐abundance nucleic acids. For clinical validation, we demonstrated that SPOT could detect serum miRNAs for the diagnostics of breast cancer, gastric cancer and prostate cancer. Furthermore, SPOT exhibits potent detection performance over SARS‐CoV‐2 RNA from clinical swabs with high sensitivity and specificity. Finally, SPOT is compatible with point‐of‐care testing modalities such as lateral flow assays. Hence, we envision that SPOT may serve as a robust assay for the sensitive detection of a variety of nucleic acid targets enabling molecular diagnostics in clinics. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
10. Reconstruction of Vesicle Assemblies with DNA Nanorulers for Resolving Heterogeneity of Vesicles in Live Cells.
- Author
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Zhang, Yueyue, Liu, Jiangbo, Mao, Xiuhai, Fan, Hongxuan, Li, Fan, Wang, Shaopeng, Li, Jiang, Li, Min, and Zuo, Xiaolei
- Published
- 2024
- Full Text
- View/download PDF
11. Data Storage Using DNA.
- Author
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Wang, Shaopeng, Mao, Xiuhai, Wang, Fei, Zuo, Xiaolei, and Fan, Chunhai
- Published
- 2024
- Full Text
- View/download PDF
12. DNA Framework-Enabled 3D Organization of Antiarrhythmic Drugs for Radiofrequency Catheter Ablation.
- Author
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Chen H, Li F, Ge Y, Liu J, Xing X, Li M, Ge Z, Zuo X, Fan C, Wang S, and Wang F
- Abstract
Preorganizing molecular drugs within a microenvironment is crucial for the development of efficient and controllable therapeutic systems. Here, the use of tetrahedral DNA framework (TDF) is reported to preorganize antiarrhythmic drugs (herein doxorubicin, Dox) in 3D for catheter ablation, a minimally invasive treatment for fast heartbeats, aiming to address potential complications linked to collateral tissue damage and the post-ablation atrial fibrillation (AF) recurrence resulting from incomplete ablation. Dox preorganization within TDF transforms its random distribution into a confined, regular spatial arrangement governed by DNA. This, combined with the high affinity between Dox and DNA, significantly increases local Dox concentration. The exceptional capacity of TDF for cellular internalization leads to a 5.5-fold increase in intracellular Dox amount within cardiomyocytes, effectively promoting cellular apoptosis. In vivo investigations demonstrate that administering TDF-Dox reduces the recurrence rate of electrical conduction after radiofrequency catheter ablation (RFCA) to 37.5%, compared with the 77.8% recurrence rate in the free Dox-treated group. Notably, the employed Dox dosage exhibits negligible adverse effects in vivo. This study presents a promising treatment paradigm that strengthens the efficacy of catheter ablation and opens a new avenue for reconciling the paradox of ablation efficacy and collateral damage., (© 2024 Wiley‐VCH GmbH.)
- Published
- 2024
- Full Text
- View/download PDF
13. Directing the Encapsulation of Single Cells with DNA Framework Nucleator-Based Hydrogel Growth.
- Author
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Wei Y, Feng Y, Wang K, Wei Y, Li Q, Zuo X, Li B, Li J, Wang L, Fan C, and Zhu Y
- Subjects
- Animals, Cell Line, DNA, Mammals, Hydrogels chemistry, Biomimetic Materials
- Abstract
Encapsulating individual mammalian cells with biomimetic materials holds potential in ex vivo cell culture and engineering. However, current methodologies often present tradeoffs between homogeneity, stability, and cell compatibility. Here, inspired by bacteria that use proteins stably anchored on their outer membranes to nucleate biofilm growth, we develop a single-cell encapsulation strategy by using a DNA framework structure as a nucleator (DFN) to initiate the growth of DNA hydrogels under cell-friendly conditions. We find that among the tested structures, the tetrahedral DFN can evenly and stably reside on cell membranes, effectively initiating hybridization chain reactions which generate homogeneously dense yet flexible single-cell encapsulation for diverse cell lines. The encapsulation persists for up to 72 hours in a serum-containing cell culture environment, representing a ~70-fold improvement compared to encapsulations mediated by single-stranded DNA nucleators. The metabolism and proliferation of the encapsulated cells are suppressed, but can be restored to the original efficiencies upon release, suggesting the superior cell compatibility of the encapsulation. We also find that compared to naked cells, the encapsulated cells exhibit a lower autophagy level after undergoing mechanical stress, suggesting the protective effect of the DNA encapsulation. This method may provide a new tool for ex vivo cell engineering., (© 2024 Wiley‐VCH GmbH.)
- Published
- 2024
- Full Text
- View/download PDF
14. A Programmable DNAzyme for the Sensitive Detection of Nucleic Acids.
- Author
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Shi C, Yang D, Ma X, Pan L, Shao Y, Arya G, Ke Y, Zhang C, Wang F, Zuo X, Li M, and Wang P
- Subjects
- RNA, Viral, Endonucleases, Nucleic Acid Amplification Techniques, DNA, Catalytic metabolism, MicroRNAs, Biosensing Techniques
- Abstract
Nucleic acids in biofluids are emerging biomarkers for the molecular diagnostics of diseases, but their clinical use has been hindered by the lack of sensitive detection assays. Herein, we report the development of a sensitive nucleic acid detection assay named SPOT (sensitive loop-initiated DNAzyme biosensor for nucleic acid detection) by rationally designing a catalytic DNAzyme of endonuclease capability into a unified one-stranded allosteric biosensor. SPOT is activated once a nucleic acid target of a specific sequence binds to its allosteric module to enable continuous cleavage of molecular reporters. SPOT provides a highly robust platform for sensitive, convenient and cost-effective detection of low-abundance nucleic acids. For clinical validation, we demonstrated that SPOT could detect serum miRNAs for the diagnostics of breast cancer, gastric cancer and prostate cancer. Furthermore, SPOT exhibits potent detection performance over SARS-CoV-2 RNA from clinical swabs with high sensitivity and specificity. Finally, SPOT is compatible with point-of-care testing modalities such as lateral flow assays. Hence, we envision that SPOT may serve as a robust assay for the sensitive detection of a variety of nucleic acid targets enabling molecular diagnostics in clinics., (© 2024 Wiley-VCH GmbH.)
- Published
- 2024
- Full Text
- View/download PDF
15. Twisted DNA Origami-Based Chiral Monolayers for Spin Filtering.
- Author
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Wang H, Yin F, Li L, Li M, Fang Z, Sun C, Li B, Shi J, Li J, Wang L, Song S, Zuo X, Liu X, and Fan C
- Subjects
- Nanotechnology, Nucleic Acid Conformation, DNA chemistry, DNA, Single-Stranded
- Abstract
DNA monolayers with inherent chirality play a pivotal role across various domains including biosensors, DNA chips, and bioelectronics. Nonetheless, conventional DNA chiral monolayers, typically constructed from single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA), often lack structural orderliness and design flexibility at the interface. Structural DNA nanotechnology has emerged as a promising solution to tackle these challenges. In this study, we present a strategy for crafting highly adaptable twisted DNA origami-based chiral monolayers. These structures exhibit distinct interfacial assembly characteristics and effectively mitigate the structural disorder of dsDNA monolayers, which is constrained by a limited persistence length of ∼50 nm of dsDNA. We highlight the spin-filtering capabilities of seven representative DNA origami-based chiral monolayers, demonstrating a maximal one-order-of-magnitude increase in spin-filtering efficiency per unit area compared with conventional dsDNA chiral monolayers. Intriguingly, our findings reveal that the higher-order tertiary chiral structure of twisted DNA origami further enhances the spin-filtering efficiency. This work paves the way for the rational design of DNA chiral monolayers.
- Published
- 2024
- Full Text
- View/download PDF
16. Programmable Atom-Like Nanoparticle Reporters for High-Precision Urinalysis of In Situ Membrane Proteins.
- Author
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Ding F, Zhang S, Chen Q, Xie X, Xi Z, Ge Z, Zuo X, Yang X, Willner I, Fan C, Li Q, and Xia Q
- Subjects
- Urinalysis, DNA chemistry, Cell Membrane, Ligands, Membrane Proteins, Nanoparticles chemistry
- Abstract
The expression of disease-specific membrane proteins (MPs) is a crucial indicator for evaluating the onset and progression of diseases. Urinalysis of in situ MPs has the potential for point-of-care disease diagnostics, yet remains challenging due to the lack of molecular reporter to transform the expression information of in situ MPs into the measurable urine composition. Herein, a series of tetrahedral DNA frameworks (TDFs) are employed as the cores of programmable atom-like nanoparticles (PANs) to direct the self-assembly of PAN reporters with defined ligand valence and spatial distribution. With the rational spatial organization of ligands, the interaction between PAN reporters and MPs exhibits superior stability on cell-membrane interface under renal tubule-mimic fluid microenvironment, thus enabling high-fidelity conversion of MPs expression level into binding events and reverse assessment of in situ MP levels via measurement of the renal clearance efficiency of PAN reporters. Such PAN reporter-mediated signal transformation mechanism empowers urinalysis of the onset of acute kidney injury at least 6 h earlier than the existing methods with an area under the curve of 100%. This strategy has the potential for urinalysis of a variety of in situ membrane proteins., (© 2023 Wiley-VCH GmbH.)
- Published
- 2024
- Full Text
- View/download PDF
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