881 results on '"Yuan, Wen"'
Search Results
2. Self-Alignment Capability Analysis and Self-Attraction Dipole Solution of Spacecraft Electromagnetic Docking
- Author
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Yuan-wen Zhang and Peng-lin Chen
- Subjects
Aerospace Engineering - Abstract
Spacecraft electromagnetic docking has many potential advantages, such as no propellant consumption and plume contamination, while providing high-precision, continuous, reversible, synchronous, and noncontacting control capabilities. With these advantages, there are strong dynamic nonlinearity and coupling, which are not friendly to controller design. Electromagnetic actuation has capabilities of force/torque symmetry and self-alignment, which could be exploited not only to simplify the controller but also to reduce the demand of relative motion measurement and improve control precision. In this paper, based on the theoretical derivation of nonlinear relative motion dynamic models, the magnetic force/torque symmetries and the self-alignment capability are analyzed, and then the self-attraction magnetic dipoles are solved. In addition, several numerical simulation cases are given to verify these capabilities and dipole solutions.
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- 2023
3. Magnetic-frozen plasma loop far-distance deliver and electromagnetic capture of companion spacecraft
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Yuan-wen Zhang and Hong-liang Zhao
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Atmospheric Science ,Geophysics ,Space and Planetary Science ,Aerospace Engineering ,General Earth and Planetary Sciences ,Astronomy and Astrophysics - Published
- 2023
4. Restricted power: Can microorganisms maintain soil organic matter stability under warming exceeding 2 degrees?
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Jie Zhou, Yuan Wen, Matthias C. Rillig, Lingling Shi, Michaela A. Dippold, Zhaohai Zeng, Yakov Kuzyakov, Huadong Zang, Davey L. Jones, and Evgenia Blagodatskaya
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Global and Planetary Change ,Ecology ,Ecology, Evolution, Behavior and Systematics - Published
- 2023
5. A feasibility study comparing car-t cell therapy and separate limb end perfusion therapy in the treatment of melanoma
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Yuan Wen
- Abstract
There are several potential advancements within cancer immunotherapy, but cells of chimeric antigen receptor-T stand out. Recently, two CAR-T cell designs targeting CD19 have been approved for use in the US and the European Union due to their promising results in the treatment of hematologic malignancies. Currently, researchers are focusing on evaluating the efficientness of CAR-T cell treatment for a variety of cancer of the solid tissues. Melanoma is caused by the malignant transformation of melanocytes. In addition to the skin, melanomas can also develop in other locations where neural crest cells migrate, such as the digestive system or the brain. Melanocytes are formed in the neural crest. Since transformed cells must overcome extra obstacles to survive, the treatment of solid cancers with cells that produce CAR-T has been less successful than the treatment of hematologic malignancies with cells that produce CAR-T. A solid cancer's immunosuppressive microenvironment and the inability to migrate cells that produce CAR-T to the site of the cancer are two significant barriers. In addition, finding the optimal target antigens to avoid on-target toxicity and non-cancer toxicity is a challenge. To lessen the harmful effects of systemic chemotherapy, Creech & Krementz developed isolated limb perfusion in 1956 to create high levels of chemotherapy in limbs affected by unresectable cancers, particularly soft tissue sarcomas and melanomas. By using these targets, it is possible to remove the wounded limb's circulation from the body's circulation and connect it to an extracorporeal system. When the patient becomes excessively hot, chemotherapeutic medications, primarily melphalan & cancer necrosis factor, are administered via a perfusion circuit. The objective of this article is to provide a summary of the benefits and drawbacks of employing car-t-cell therapy to cure solid cancers, particularly melanoma. It also investigates the possibilities of curing melanoma using car-T treatment as well as isolated limb perfusion.
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- 2023
6. Ligand-Promoted Bifunctional Cobalt-Catalyzed Carbonylation-Polymerization of Epoxides: One Step to Polyhydroxyalkanoates
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Yuan Wen, Ruifang Nie, Bo Li, and Suhua Li
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General Chemistry ,Catalysis - Published
- 2023
7. Blood Oxygen Saturation and Heart Rate Monitor for Home-Based Continuous Monitoring
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Nurul Izzati Darul Zaman, Siti Nurhaliza Zainal Abiden, Kok Wai Soo, Ming Chern Leong, and Yuan Wen Hau
- Abstract
Pneumonia and COVID-19 are the most infected infectious diseases that deteriorate health of all range of people. If the early treatment is not provided, it may lead to severe heart disease and eventually to mortality. Therefore, continuous heart rate (HR), as well as blood oxygen saturation (SpO2) monitoring can help to detect abnormalities early. As a result, this paper presents a combination of portable continuous SpO2 and HR monitor which focuses on the improvement of internal design and graphical user interface of the mobile application. In this system, MAX30100, a photoplethysmography (PPG) sensor, was used to measure SpO2 and HR. Then, the signal was transmitted to Arduino Nano as the master microcontroller to process all acquired data from the sensor. The device was integrated with Android-based mobile application through Bluetooth communication, for displaying the result of the measured real-time parameters continuously and for battery status indication. Quantitative measurements showed that the precision of the circuit system implemented for HR and SpO2 were 99.16% and 99.06%, respectively. This system would improve the working efficiency and life efficiency of the healthcare provider and public.
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- 2023
8. Thermoelectric properties of solution-prepared n-type and p-type CZTSSe thin films
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Yuan-Wen Hsiao, Shang-Ren Chee, Hsuan-Ta Wu, and Chuan-Feng Shih
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Process Chemistry and Technology ,Materials Chemistry ,Ceramics and Composites ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials - Published
- 2023
9. A molecular signature defining exercise adaptation with ageing and in vivo partial reprogramming in skeletal muscle
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Ronald G. Jones, Andrea Dimet‐Wiley, Amin Haghani, Francielly Morena da Silva, Camille R. Brightwell, Seongkyun Lim, Sabin Khadgi, Yuan Wen, Cory M. Dungan, Robert T. Brooke, Nicholas P. Greene, Charlotte A. Peterson, John J. McCarthy, Steve Horvath, Stanley J. Watowich, Christopher S. Fry, and Kevin A. Murach
- Subjects
Physiology - Abstract
Advances in the last decade related to cellular epigenetic reprogramming (e.g. DNA methylome remodeling) toward a pluripotent state via the Yamanaka transcription factors Oct3/4, Klf4, Sox2, and Myc (OKSM) provide a window into potential mechanisms for combatting the deleterious effects of cellular ageing Using global gene expression analysis, we compared the effects of in vivo OKSM-mediated partial reprogramming in skeletal muscle fibres of mice to the effects of late-life murine exercise training in muscle Myc is the Yamanaka factor most induced by exercise in skeletal muscle, so we compared the MYC-controlled transcriptome in muscle to Yamanaka factor-mediated and exercise adaptation gene landscapes in mice and humans A single pulse of MYC is sufficient to remodel the muscle methylome We identify partial reprogramming-associated genes that are innately altered by exercise training and conserved in humans, and propose that MYC contributes to some of these responses ABSTRACT: Exercise promotes functional improvements in aged tissues, but the extent to which it simulates partial molecular reprogramming is unknown. Using transcriptome profiling from 1) a skeletal muscle-specific in vivo Oct3/4, Klf4, Sox2, and Myc (OKSM) reprogramming-factor expression murine model, 2) an in vivo inducible muscle-specific Myc induction murine model, 3) a translatable high-volume hypertrophic exercise training approach in aged mice, and 4) human exercise muscle biopsies, we collectively defined exercise-induced genes that are common to partial reprogramming. Late-life exercise training lowered murine DNA methylation age according to several contemporary muscle-specific clocks. A comparison of the murine soleus transcriptome after late-life exercise training to the soleus transcriptome after OKSM induction revealed an overlapping signature that included higher JunB and Sun1. Also, within this signature, downregulation of specific mitochondrial and muscle-enriched genes was conserved in skeletal muscle of long-term exercise-trained humans; among these was muscle-specific Abra/Stars. Myc is the OKSM factor most induced by exercise in muscle and was elevated following exercise training in aged mice. A pulse of MYC rewired the global soleus muscle methylome, and the transcriptome after a MYC pulse partially recapitulated OKSM induction. A common signature also emerged in the murine MYC-controlled and exercise adaptation transcriptomes, including lower muscle-specific Melusin and reactive oxygen species-associated Romo1. With Myc, OKSM, and exercise training in mice as well habitual exercise in humans, the complex I accessory subunit Ndufb11 was lower; low Ndufb11 is linked to longevity in rodents. Collectively, exercise shares similarities with genetic in vivo partial reprogramming. Abstract figure legend Diverse forms of exercise training improve muscle function and whole-body health, even if initiated late in life. Information on conserved exercise-controlled molecular cues that underpin a younger muscle phenotype in aged muscle has potential utility in the development of anti-ageing therapies. Induction of the Yamanaka factors Oct3/4, Klf4, Sox2, and Myc are known to ameliorate ageing hallmarks. Comparison of transcriptomic data from aged exercise-trained mice and humans to muscle fibre-specific genetically driven models of epigenetic reprogramming (e.g. Yamanaka factor or Myc expression) unearthed conserved biomarkers associated with molecular age mitigation. Considering reduced biological age according to DNA methylome analysis, high-volume exercise training can be classified as an epigenetic reprogramming stimulus. Chronic exercise should be considered alongside and/or as a method to inform healthspan-extending longevity approaches such as pharmacologic and dietary interventions. This article is protected by copyright. All rights reserved.
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- 2023
10. Evaluation of Haplo-Paraspinal-Muscle-Preserving Technique to Prevent Postoperative Axial Pain in Cervical Laminoplasty
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Shen, XiaoLong, Xu, Chen, Wang, Ruizhe, Zhang, Zifan, Qi, Min, Zhang, Yizhi, Wu, Huiqiao, Liu, Yang, Chen, Huajiang, and Yuan, Wen
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Cervical Spine ,Orthopedics and Sports Medicine ,Surgery - Abstract
BACKGROUND: The present study aimed to assess the efficacy of a new haplo-paraspinal-muscle-preserving (HMP) laminoplasty technique in the treatment of cervical myelopathy. METHODS: The medical records of 68 patients diagnosed with multisegmental cervical myelopathy were retrospectively reviewed. Of these, 22 patients who underwent HMP laminoplasty were defined as the muscle-preserved group (MP), and 46 patients who underwent traditional open-door laminoplasty were enrolled and defined as the traditional open-door laminoplasty group (LP). Patient demographic data and surgical parameters like clinical and radiological parameters, operation duration, blood loss, and spinal canal expansion distance were compared. RESULTS: Average surgical time and blood loss were significantly reduced in the MP group when compared with the LP group (P < 0.05). Both groups demonstrated significant improvements in neurological function and spinal canal expansion (P > 0.05). However, the visual analog scale score in the MP group was significantly lower compared with the LP group at the 6-month follow-up (P < 0.05), but no differences were found at the 1-year follow-up. The loss of lordosis was more prominent in the LP group when compared with the MP group at 1-year follow-up (P < 0.05). Lower events of persistent axial pain were found in the MP group but with no statistical significance. More hinge side laminae fractures could be found in the MP group, but more hinge side displacements were found in the LP group. CONCLUSIONS: The HMP laminoplasty technique is relatively safe, effective, easier to perform, and better for lordosis maintenance and complication control compared with the traditional open-door technique. CLINICAL RELEVANCE: Although traditional open-door laminoplasty is an efficient approach in treating multisegmental cervical myelopathy, the complications could significantly affect the clinical outcome. Our new HMP laminoplasty technique has a lower complication rate and a better lordosis maintenance ability; therefore, it could be a better choice in treating multisegmental cervical myelopathy. LEVEL OF EVIDENCE: 3.
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- 2023
11. The incorporation of straw into the subsoil increases C, N, and P enzyme activities and nutrient supply by enriching distinctive functional microorganisms
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Deqiang Zhao, Jun Ling, Gong Wu, Zixi Liu, Shunli Zhou, Yuan Wen, and Lukas Beule
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Soil Science ,Environmental Chemistry ,Development ,General Environmental Science - Published
- 2022
12. Phytochemicals, Biological Activities, Molecular Mechanisms, and Future Prospects of Plantago asiatica L
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Shi-Yuan Wen, Bing-Yan Wei, Jie-Qiong Ma, Li Wang, and Yan-Yan Chen
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General Chemistry ,General Agricultural and Biological Sciences - Published
- 2022
13. Short‐term passenger flow prediction for rail transit based on improved particle swarm optimization algorithm
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Yuan‐wen Lai and Yang Wang
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Mechanical Engineering ,Transportation ,Law ,General Environmental Science - Published
- 2022
14. Upconversion Luminescence via Anion Exchange in Perovskite Quantum Dots for Anticounterfeiting Inkjet Printing
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Xiang Zheng, Yuan Wen, Jun Zhong, and Ai-Zheng Chen
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General Chemical Engineering ,General Chemistry - Abstract
Lanthanide-doped upconversion nanoparticles (UCNPs) and cesium lead halide perovskite quantum dots (PeQDs) are highly compatible with each other: UCNPs produce anti-Stokes upconversion luminescence (UCL) under near-infrared (NIR) excitation and the emissive profiles of PeQDs can be conveniently tuned by varying the halide composition ratio. Therefore, in this study, UCNPs and PeQDs are mixed together, producing colorful UCL under 980 nm laser excitation. In addition, ZnI
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- 2022
15. Hybrid models of subway passenger flow prediction based on convolutional neural network
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Yuan‐wen Lai, Yang Wang, Xin‐ying Xu, Said M. Easa, and Xiao‐wei Zhou
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Mechanical Engineering ,Transportation ,Law ,General Environmental Science - Published
- 2022
16. Mobile Electrocardiogram Monitoring System with Cloud-Based Approach
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Voon Hueh Goh, Muhammad Akmal Ayob, Nurul Izzati Darul Zaman, and Yuan Wen Hau
- Abstract
Cardiovascular disease (CVD) is a heart related disease and is the top silent killer in worldwide. Frequent electrocardiogram (ECG) monitoring for patients with CVD is vital to check if arrhythmia occurs. Though there are lots of commercialized portable ECG monitoring device available, many of them are dedicated to professional clinical staff with complex user interface, or only targeted to specific arrhythmia for home monitoring with lack of data management system. In this study, an in-house developed ECG monitoring device was integrated with an Android-based mobile application through Bluetooth wireless communication and implements Google Cloud Technology. The Android-based mobile app supports main features of real-time ECG graph display, automated location detector, data management system and simple arrhythmia detection. The ECG graph displays acquired ECG signal in real-time by in-house ECG acquisition unit through Bluetooth wireless communication and stored as text files in phone’s local memory. Firebase Authentication and Firebase Storage based on Google Cloud technology are implemented for data management system development. This allows users and administrators to upload or access data securely through online Google Cloud Platform. Three types of heart rhythm, namely normal sinus rhythm, bradycardia and tachycardia can be classified based on heart rate analysis. Smartphone’s location service is enabled to allow user shares their location with caretaker in emergency case. The final solution utilizes the Internet-of-Things (IoT) technology to facilitate heart disease management towards telemedicine applications.
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- 2022
17. Immune checkpoint inhibitors combined with radiotherapy/chemoradiotherapy in treating locally advanced or recurrent/metastatic esophageal squamous cell carcinoma: A real-world study
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Xiao-Han Zhao, Hong-mei Gao, Jing-Yuan Wen, He-Song Wang, Luan-ying Wu, Chun-Yang Song, Wen-Zhao Deng, Shu-Chai Zhu, and Wen-Bin Shen
- Abstract
Objective: This study was designed to investigate the prognostic factors for immune checkpoint inhibitors (ICIs) combined utilization with radiotherapy (RT)/chemoradiotherapy (CRT) and to evaluate their toxicity in locally advanced or recurrent/metastatic esophageal squamous cell carcinoma (LA/RM ESCC). Methods: In this study, 198 LA/RM ESCC patients who received ICIs combined with RT/CRT in the Department of Radiotherapy of the Fourth Hospital of Hebei Medical University were retrospectively analyzed. Univariate and multivariate analyses were performed to determine the prognostic factors for overall survival (OS) and progression-free survival (PFS). Subgroup analysis was utilized to explore the prognostic factors, the treatment respond and treatment-related adverse events (trAEs) were analyzed. Results: The median OS and PFS were 30.4 months (95% confidence interval [CI]: 15.1–45.7 months) and 15.3 months (95% CI: 12.8–17.8 months), respectively. The median OS and PFS for patients achieving objective respond (ORR group, including complete response and partial response) were 50.8 months (95% CI: 25.8–75.7 months) and 20.5 months (95% CI: 14.1–27.0), respectively, which were higher than those in non-ORR group (OSnon-ORR:17.5 months, 95% CI: 14.0–21.0; χ2 = 13.881, P < 0.001; PFSnon-ORR: 12.1 months, 95% CI: 10.1–14.1, χ2 = 10.676, P = 0.001). Results from subgroup analysis illustrated combined ICIs with radiotherapy could improve the treatment respond (χ2 = 47.725, P = 0.000). The OS rate (χ2 = 18.836, P < 0.001) and PFS rate (χ2 = 6.881, P = 0.009) were significantly higher in the whole-lesion radiotherapy group than in the partial-lesion radiotherapy group. Multivariate analysis showed that the number of immune cycles, the coverage of radiotherapy target, the modality of ICIs-combination therapy and treatment response were independent prognosis factors for OS (hazard ratio [HR] = 0.512, 2.043, 1.889, and 1.912, respectively; P = 0.001, 0.001, 0.013, and 0.002, respectively). Radiotherapy coverage and treatment response were independent factors for PFS (HR = 1.478 and 1.597, respectively; P = 0.032 and 0.012, respectively). In the entire study population, 83 patients (41.9%) had ≥ grade 2 trAEs. Conclusions: ICIs combined with radiotherapy/chemoradiotherapy are safe and effective in LA/RM ESCC patients. The addition of radiotherapy could improve the treatment respond and whole-lesion radiotherapy improve prognosis compared with partial-lesion radiotherapy group. The number of immunotherapy cycles and treatment response are the main factors affecting prognosis.
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- 2023
18. Higher temperature variability in deforested mountain regions impacts the competitive advantage of nocturnal species
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Shih-Fan Chan, Dustin R. Rubenstein, I-Ching Chen, Yu-Meng Fan, Hsiang-Yu Tsai, Yuan-Wen Zheng, and Sheng-Feng Shen
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General Immunology and Microbiology ,General Medicine ,General Agricultural and Biological Sciences ,General Biochemistry, Genetics and Molecular Biology ,General Environmental Science - Abstract
Deforestation is a major contributor to biodiversity loss, yet the impact of forest loss on daily microclimate variability and its implications for species with different daily activity patterns remain poorly understood. Using a recently developed microclimate model, we investigated the effects of deforestation on the daily temperature range (DTR) in low-elevation tropical regions and high-elevation temperate regions. Our results show that deforestation substantially increases DTR in these areas, suggesting a potential impact on species interactions. To test this hypothesis, we studied the competitive interactions between nocturnal burying beetles and all-day-active blowfly maggots in forested and deforested habitats in Taiwan. We show that deforestation leads to increased DTR at higher elevations, which enhances the competitiveness of blowfly maggots during the day and leads to a higher failure rate of carcass burial by the beetles at night. Thus, deforestation-induced temperature variability not only modulates exploitative competition between species with different daily activity patterns, but also likely exacerbates the negative impacts of climate change on nocturnal organisms. In order to limit potential adverse effects on species interactions and their ecological functions, our study highlights the need to protect forests, especially in areas where deforestation can greatly alter temperature variability.
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- 2023
19. Zn-Mediated Scalable Synthesis of Trifluoromethylphosphines from Phosphine Chlorides and CF3Br
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Deqian Peng, Shouzhi Zhang, Wu Fan, Yuan Wen, and Suhua Li
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Organic Chemistry ,Physical and Theoretical Chemistry ,Biochemistry - Published
- 2023
20. Correction to: Guillain–Barre syndrome after myocardial infarction: a case report and literature review
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Pu-yuan Wen, Xian-wen Chen, Min Zhang, Wen-zheng Chu, Hong-liang Wu, and Chao Ren
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Cardiology and Cardiovascular Medicine - Published
- 2023
21. To wear or not to wear? The influence of regulatory focus and individual cultural orientation on face mask wearing
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Yuan Wen, Shipra Gupta, and Merve Coskun
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Sociology and Political Science ,General Economics, Econometrics and Finance - Published
- 2023
22. A Rejuvenation Signature in Skeletal Muscle That Is Mediated By Late-Life Exercise
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Ronald Jones, Andrea Dimet-Wiley, Amin Haghani, Francielly Morena da Silva, Camille Brightwell, Seongkyun Lim, Sabin Khadgi, Yuan Wen, Cory Dungan, Robert Brooke, Nicholas Greene, Charlotte Peterson, John McCarthy, Steve Horvath, Stanley Watowich, Christopher Fry, and Kevin Murach
- Subjects
Physiology - Abstract
The objectives of this study were to measure to what extent exercise stimulates partial molecular reprogramming in skeletal muscle. We hypothesized that exercise training, beyond its known functional improvements in aged tissues, could recapitulate some of the same effects that are seen with in vivo partial reprogramming by Yamanaka factors. Using transcriptome profiling from 1) a skeletal muscle-specific in vivo Oct3/4, Klf4, Sox2, and Myc (OKSM) reprogramming-factor expression murine model, 2) an in vivo inducible muscle-specific Myc induction murine model, 3) a translatable high-volume hypertrophic exercise training approach in aged mice, and 4) human exercise muscle biopsies, we collectively defined exercise-induced genes common to partial reprogramming. Late-life exercise training lowered murine DNA methylation age according to several contemporary muscle-specific clocks. A comparison of the murine soleus transcriptome after late-life exercise training to the soleus transcriptome after OKSM induction revealed an overlapping signature. Within this signature, downregulation of specific mitochondrial and muscle-enriched genes was conserved in skeletal muscle of long-term exercise-trained humans; among these was muscle-specific Abra/Stars. Myc is the OKSM factor most induced by exercise in muscle and was elevated following exercise training in aged mice. A pulse of MYC rewired the global soleus muscle methylome, and the transcriptome after a MYC pulse partially recapitulated OKSM induction. A common signature also emerged in the murine MYC-controlled and exercise adaptation transcriptomes, including lower muscle-specific Melusin and reactive oxygen species-associated Romo1. With Myc, OKSM, and exercise training in mice as well habitual exercise in humans, the complex I accessory subunit Ndufb11 was lower; low Ndufb11 is linked to longevity in rodents. Our data collectively suggest: 1) a biological age-mitigating effect on the epigenetic landscape by late-life exercise-training in murine skeletal muscle, 2) a common gene expression signature of partial reprogramming by OKSM and exercise training in muscle of humans and aged mice, and 3) that Myc is an exercise-responsive factor that contributes to a rewired molecular profile at the transcriptome and methylome levels. National Institutes of Health: Kevin A. Murach, AG063994; American Federation for Aging Research (AFAR): Kevin A. Murach, Junior Investigator Grant This is the full abstract presented at the American Physiology Summit 2023 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.
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- 2023
23. Guillain–Barre syndrome after myocardial infarction: a case report and literature review
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Pu-yuan Wen, Xian-wen Chen, Min Zhang, Wen-zheng Chu, Hong-liang Wu, and Chao Ren
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Cardiology and Cardiovascular Medicine - Abstract
Background Guillain–Barre syndrome after myocardial infarction occurs infrequently, and its occurrence following percutaneous coronary intervention is extremely rare. Due to the high mortality rate of myocardial infarction and the disability of Guillain–Barre syndrome, early identification of Guillain–Barre syndrome after myocardial infarction and early intervention can decrease the mortality rate, lead to early recovery, and provide a better outcome. Case presentation Herein, we reported a rare case of Guillain–Barre syndrome after myocardial infarction treated with percutaneous coronary intervention. The patient was a 75-year-old woman from China who was admitted to hospital due to sudden loss of consciousness. Electrocardiography showed acute myocardial infarction in the right ventricle and inferior and posterior walls. The patient underwent emergency percutaneous intervention of the posterior collateral artery of the right coronary artery. Soon after, her condition worsened resulting in limb weakness and numbness. Unfortunately, she continued to develop respiratory failure, and treated with intravenous immunoglobulin and ventilator-assisted breathing. A physical examination showed hypotonia of all four limbs, complete quadriplegia, bulbar palsy, dysarthria, and tendon areflexia. Serum immunoglobulin (Ig) G anti-ganglioside antibody analysis was positive with anti-GT1a antibodies (+ +), anti-GM1 antibodies ( +), anti-GM2 antibodies ( +), and anti-GM4 antibodies ( +), and he was diagnosed with Guillain–Barre syndrome after myocardial infarction. She was discharged due to poor response to treatment. The patient died two days after being discharged. Conclusions Myocardial infarction and/or percutaneous coronary intervention may activate immune-mediated response and cause severe complications. Clinician should be alert to Guillain–Barre syndrome after myocardial infarction and/or percutaneous coronary intervention.
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- 2023
24. Polyphyllin D 'Punctures' Hypertrophic Lysosomes to Reverse Drug Resistance of Hepatocellular Carcinoma by Targeting Acid Sphingomyelinase
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Yang Wang, Yan-Yan Chen, Gui-Bin Gao, Yang-Han Zheng, Nan-Nan Yu, Lan Ouyang, Xuejuan Gao, Nan Li, Shi-Yuan Wen, Shangjia Huang, Qian Zhao, Langxia Liu, Mingrong Cao, Shuixing Zhang, Jing Zhang, and Qing-Yu He
- Subjects
Pharmacology ,Drug Discovery ,Genetics ,Molecular Medicine ,Molecular Biology - Published
- 2023
25. Effect of lamellae thickness on bubble dissolution in rotomoulding
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Kejie Xu, Yuan Wen, Deyu Guo, and Xiaoyang Yan
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Polymers and Plastics ,Materials Chemistry ,General Chemistry - Published
- 2022
26. Smoking increases the risk of postoperative wound complications: A propensity score‐matched cohort study
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Yu‐Hsuan Fan Chiang, Yuan‐Wen Lee, Fai Lam, Chien‐Chang Liao, Chuen‐Chau Chang, and Chao‐Shun Lin
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Surgery ,Dermatology - Abstract
Cigarette smoking is associated with surgical complications, including wound healing and surgical site infection. However, the association between smoking status and postoperative wound complications is not completely understood. Our objective was to investigate the effect of smoking on postoperative wound complications for major surgeries. Data were collected from the 2013 to 2018 participant use files of the American College of Surgeons National Surgical Quality Improvement Program database. A propensity score matching procedure was used to create the balanced smoker and nonsmoker groups. Multivariable logistic regression was used to calculate the odds ratios (ORs) with 95% confidence intervals (CIs) for postoperative wound complications, pulmonary complications, and in-hospital mortality associated with smokers. A total of 1 156 002 patients (578 001 smokers and 578 001 nonsmokers) were included in the propensity score matching analysis. Smoking was associated with a significantly increased risk of postoperative wound disruption (OR 1.65, 95% CI 1.56-1.75), surgical site infection (OR 1.31, 95% CI 1.28-1.34), reintubation (OR 1.47, 95% CI 1.40-1.54), and in-hospital mortality (OR 1.13, 95% CI 1.07-1.19) compared with nonsmoking. The length of hospital stay was significantly increased in smokers compared with nonsmokers. Our analysis indicates that smoking is associated with an increased risk of surgical site infection, wound disruption, and postoperative pulmonary complications. The results may drive the clinicians to encourage patients to quit smoking before surgery.
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- 2022
27. INFLUENCE OF SOCIAL MEDIA USAGE ON ORGANIZATIONAL PERFORMANCE AN EMPIRICAL STUDY FROM ENTERPRISE IN GHANA
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null Li Yuan Wen and null Takyi Kwabena Nsiah
- Abstract
The purpose of the article is to investigate social media use (Face book, Twitter, You Tube, Instagram) and its influence on companies in Ghana. The research selected eight firms from each of the 16 regions in Ghana. A total of 128 questionnaires were administered to the companies. By employing the Smart PLS3, the article analysis the proposed hypothesis developed for the research. The findings of the investigations indicated that cost benefit, interactivity, management support, entrepreneurial orientation had an insignificant affiliation with social media use. However, environmental factors such as competition, supplier needs, and customers needs had a positive and a material nexus with social media usage. Finally, social media use had a positive and a favorable association effect on the organisations. This article recommend that organizations may use social media to get a lot of information about their consumers and rivals. Customers’ preferences, requirements, expectations, and frustrations are all understood by them. Social media aids in the retrieval of more information about the company’s rivals, their actions, strategies, and brand feelings, allowing them to improve their goods and services and have a bigger influence on the company’s performance. KEYWORDS: Social media usage, organizational performance, enterprise in Ghana, environmental factors, technological factors, organizational factors.
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- 2022
28. Multiple lines of evidence supports the two varieties of Halenia elliptica (Gentianaceae) as two species
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Dong-Rui Jia, Yuan-Wen Duan, Ruijuan Liu, Zhi-Li Zhou, Jin-Feng Wu, Min-Yu Chen, Lin-Lin Wang, and Lihua Meng
- Subjects
Gentianaceae ,education.field_of_study ,biology ,Pollination ,Population ,Outcrossing ,Plant Science ,Reproductive isolation ,biology.organism_classification ,Monophyly ,Halenia ,Pollinator ,Botany ,education ,Ecology, Evolution, Behavior and Systematics - Abstract
Delimiting species requires multiple sources of evidence. Here, we delimited two varieties of Halenia elliptica (Gentianaceae) using several lines of evidence, including morphological traits and mating system in a sympatric population, phylogenetic relationships based on nrITS and cpDNA (rpl16) data, and complete chloroplast genome sequences. Comparative analysis of 21 morphological traits clearly separates the two varieties of H. elliptica. Examination of the flowering process and pollination treatments indicate that H. elliptica var. grandiflora produces seeds via outcrossing, whereas H. elliptica var. elliptica produces seeds via mixed mating. Furthermore, hand-pollinated hybridization of the two varieties produced no seeds. Observations of pollinators showed that when bees began a pollination bout on H. elliptica var. grandiflora they preferred to continue pollinating this variety; however, when they began a pollination bout on H. elliptica var. elliptica, they showed no preference for either variety. Phylogenetic analysis confirmed the monophyly of H. elliptica, which was further divided into two monophyletic clades corresponding to the two varieties. A large number of variants from the chloroplast genomes reflected remarkable genetic dissimilarities between the two varieties of H. elliptica. We recommend that the two varieties of H. elliptica should be revised as two species (H. elliptica and Halenia grandiflora). Our findings indicate that H. elliptica varieties may have split into two separate species due to a shift in mating system, changes in flowering phenology and/or post-pollination reproductive isolation.
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- 2022
29. Early transcriptomic signatures and biomarkers of renal damage due to prolonged exposure to embedded metal
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Yuan Wen, Ivan J. Vechetti, Dongliang Leng, Alexander P. Alimov, Taylor R. Valentino, Xiaohua D. Zhang, John J. McCarthy, and Charlotte A. Peterson
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Health, Toxicology and Mutagenesis ,Cell Biology ,Toxicology - Published
- 2023
30. Does competitive asymmetry confer polyploid advantage under changing environments?
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Wen Guo, Na Wei, Guang‐You Hao, Shi‐Jian Yang, Zhi‐Yong Zhu, Yong‐Ping Yang, and Yuan‐Wen Duan
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Ecology ,Plant Science ,Ecology, Evolution, Behavior and Systematics - Published
- 2023
31. Spodoptera litura larvae are attracted by <scp>HvAV</scp> ‐3h‐infected S. litura larvae‐damaged pepper leaves
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Lang Ming, Yuan‐Wen Du, Ge‐Ge Yuan, Qi Su, Xiao‐Bing Shi, Huan Yu, and Gong Chen
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Insect Science ,General Medicine ,Agronomy and Crop Science - Published
- 2023
32. The relationship between eHealth literacy and palliative care knowledge, attitudes, and practice among nurses: a cross-sectional study
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Niu Yuan, Zhang-Hong Lv, Yuan-Yuan Wen, Chun-Rong Sun, Ting-Yu Tao, and Dan Qian
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General Nursing - Abstract
Background The crucial role that nurses play in offering palliative care to patients with life-threatening diseases is widely acknowledged, but the correlation between their eHealth literacy and their knowledge, attitudes, and practice in this domain has yet to be investigated. This study is conducted to investigate the status of eHealth literacy and knowledge, attitudes, and practice regarding palliative care among nurses, and to examine their relationship. Methods A cross-sectional study design was conducted among 546 nurses selected from the first-class tertiary hospitals located both inside and outside of Zhejiang Province between May 12 and May 20, 2022. The online survey of eHealth literacy scale (eHEALS) and scale of knowledge, attitudes, and practice (KAP) regarding palliative care was performed using snowball sampling through the WeChat mini program “Questionnaire Star”. The Spearman rank correlation and binary logistic regression model were used to analyze the independent association between eHealth literacy and KAP toward palliative care. Results The median scores of eHEALS and KAP regarding palliative care were 32 (interquartile range[IQR] 29 to 38) and 82 (IQR 54 to 106) points. The results of correlation analysis showed that the KAP regarding palliative care was significantly correlated with eHEALS (rho = 0.189, P OR = 2.109; P Conclusion Nurses who worked in first-class tertiary hospitals have good levels of eHealth literacy, while the overall level of KAP regarding palliative care is moderate. Our findings highlight that the eHEALS score is independently associated with the KAP score regarding palliative care. Therefore, nursing managers should adopt multiple measures to comprehensively improve eHealth literacy among nurses, further enrich their knowledge of palliative care, promote a positive transformation of attitudes towards palliative care, and efficiently implement palliative care practice, in order to promote high-quality development of palliative care.
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- 2023
33. Environmental exposures in early-life and general health in childhood
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Amine, Ines, Guillien, Alicia, Philippat, Claire, Anguita-Ruiz, Augusto, Casas, Maribel, de Castro, Montserrat, Dedele, Audrius, Garcia-Aymerich, Judith, Granum, Berit, Grazuleviciene, Regina, Heude, Barbara, Haug, Line Småstuen, Julvez, Jordi, López-Vicente, Mónica, Maitre, Léa, McEachan, Rosemary, Nieuwenhuijsen, Mark, Stratakis, Nikos, Vafeiadi, Marina, Wright, John, Yang, Tiffany, Yuan, Wen Lun, Basagaña, Xavier, Slama, Rémy, Vrijheid, Martine, and Siroux, Valérie
- Subjects
Exposome ,Respiratory diseases ,Pregnancy ,Neurodevelopment ,Cohort studies ,Multimorbidity ,Environment ,Cardiometabolic risk factors ,Child ,General health status - Abstract
Background Early-life environmental exposures are suspected to be involved in the development of chronic diseases later in life. Most studies conducted so far considered single or few exposures and single-health parameter. Our study aimed to identify a childhood general health score and assess its association with a wide range of pre- and post-natal environmental exposures. Methods The analysis is based on 870 children (6–12 years) from six European birth cohorts participating in the Human Early-Life Exposome project. A total of 53 prenatal and 105 childhood environmental factors were considered, including lifestyle, social, urban and chemical exposures. We built a general health score by averaging three sub-scores (cardiometabolic, respiratory/allergy and mental) built from 15 health parameters. By construct, a child with a low score has a low general health status. Penalized multivariable regression through Least Absolute Shrinkage and Selection Operator (LASSO) was fitted in order to identify exposures associated with the general health score. Findings: The results of LASSO show that a lower general health score was associated with maternal passive and active smoking during pregnancy and postnatal exposure to methylparaben, copper, indoor air pollutants, high intake of caffeinated drinks and few contacts with friends and family. Higher child’s general health score was associated with prenatal exposure to a bluespace near residency and postnatal exposures to pets, cobalt, high intakes of vegetables and more physical activity. Against our hypotheses, postnatal exposure to organochlorine compounds, perfluorooctanoate and childhood medium intake of sodas and bakery products were associated with a higher child’s general health score. Conclusion By using a general health score summarizing the child cardiometabolic, respiratory/allergy and mental health, this study reinforced previously suspected environmental factors associated with various child health parameters (e.g. tobacco, air pollutants) and identified new factors (e.g. pets, bluespace) warranting further investigations.
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- 2023
34. Machine learning classifiers for screening nonalcoholic fatty liver disease in general adults
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Shenghua Qin, Xiaomin Hou, Yuan Wen, Chunqing Wang, Xiaxian Tan, Hao Tian, Qingqing Ao, Jingze Li, and Shuyuan Chu
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Multidisciplinary - Abstract
Nonalcoholic fatty liver disease (NAFLD) is one of major causes of end-stage liver disease in the coming decades, but it shows few symptoms until it develops into cirrhosis. We aim to develop classification models with machine learning to screen NAFLD patients among general adults. This study included 14,439 adults who took health examination. We developed classification models to classify subjects with or without NAFLD using decision tree, random forest (RF), extreme gradient boosting (XGBoost) and support vector machine (SVM). The classifier with SVM was showed the best performance with the highest accuracy (0.801), positive predictive value (PPV) (0.795), F1 score (0.795), Kappa score (0.508) and area under the precision-recall curve (AUPRC) (0.712), and the second top of area under receiver operating characteristic curve (AUROC) (0.850). The second-best classifier was RF model, which was showed the highest AUROC (0.852) and the second top of accuracy (0.789), PPV (0.782), F1 score (0.782), Kappa score (0.478) and AUPRC (0.708). In conclusion, the classifier with SVM is the best one to screen NAFLD in general population based on the results from physical examination and blood testing, followed by the classifier with RF. Those classifiers have a potential to screen NAFLD in general population for physician and primary care doctors, which could benefit to NAFLD patients from early diagnosis.
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- 2023
35. Gustilo IIIB, AO/OTA C3 patella fracture treated via three-step strategy: A case report
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Ze Zhang, Feng-Po Sun, Tong-Yi Zhang, and Liang-Yuan Wen
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Surgery - Published
- 2023
36. Vertical‐type 3D/Quasi‐2D n‐p Heterojunction Perovskite Photodetector
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Yuan‐Wen Hsiao, Bi Shane Cheng, Hung‐Chieh Hsu, Shih‐Hsiung Wu, Hsuan‐Ta Wu, Ching‐Chich Leu, and Chuan‐Feng Shih
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Biomaterials ,Electrochemistry ,Condensed Matter Physics ,Electronic, Optical and Magnetic Materials - Published
- 2023
37. Post-acute COVID-19 symptom risk in hospitalized and non-hospitalized COVID-19 survivors: A systematic review and meta-analysis
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Niu Yuan, Zhang-Hong Lv, Chun-Rong Sun, Yuan-Yuan Wen, Ting-Yu Tao, Dan Qian, Fang-Ping Tao, and Jia-Hui Yu
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Public Health, Environmental and Occupational Health - Abstract
BackgroundPost-acute coronavirus disease 2019 (COVID-19) symptoms occurred in most of the COVID-19 survivors. However, few studies have examined the issue of whether hospitalization results in different post-acute COVID-19 symptom risks. This study aimed to compare potential COVID-19 long-term effects in hospitalized and non-hospitalized COVID-19 survivors.MethodsThis study is designed as a systematic review and meta-analysis of observational studies. A systematic search of six databases was performed for identifying articles published from inception until April 20th, 2022, which compared post-acute COVID-19 symptom risk in hospitalized and non-hospitalized COVID-19 survivors using a predesigned search strategy included terms for SARS-CoV-2 (eg, COVID, coronavirus, and 2019-nCoV), post-acute COVID-19 Syndrome (eg, post-COVID, post COVID conditions, chronic COVID symptom, long COVID, long COVID symptom, long-haul COVID, COVID sequelae, convalescence, and persistent COVID symptom), and hospitalization (hospitalized, in hospital, and home-isolated). The present meta-analysis was conducted according to The Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) 2020 statement using R software 4.1.3 to create forest plots. Q statistics and the I2 index were used to evaluate heterogeneity in this meta-analysis.ResultsSix observational studies conducted in Spain, Austria, Switzerland, Canada, and the USA involving 419 hospitalized and 742 non-hospitalized COVID-19 survivors were included. The number of COVID-19 survivors in included studies ranged from 63 to 431, and follow-up data were collected through visits in four studies and another two used an electronic questionnaire, visit and telephone, respectively. Significant increase in the risks of long dyspnea (OR = 3.18, 95% CI = 1.90–5.32), anxiety (OR = 3.09, 95% CI = 1.47–6.47), myalgia (OR = 2.33, 95% CI = 1.02–5.33), and hair loss (OR = 2.76, 95% CI = 1.07–7.12) risk were found in hospitalized COVID-19 survivors compared with outpatients. Conversely, persisting ageusia risk was significantly reduced in hospitalized COVID-19 survivors than in non-hospitalized patients.ConclusionThe findings suggested that special attention and patient-centered rehabilitation service based on a needs survey should be provided for hospitalized COVID-19 survivors who experienced high post-acute COVID-19 symptoms risk.
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- 2023
38. Pathogenic free-living amoebic encephalitis from 48 cases in China: A systematic review
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Xiang-Ting Chen, Qian Zhang, Si-Yuan Wen, Fei-Fei Chen, and Chang-Qing Zhou
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Neurology ,Neurology (clinical) - Abstract
BackgroundFree-living amoebae (FLA) including Naegleria fowleri, Acanthamoeba spp., and Balamuthia mandrillaris can become pathogenic and cause severe cerebral infections, named primary amoebic meningoencephalitis (PAM), granulomatous amoebic encephalitis (GAE), and balamuthia amoebic encephalitis (BAE), respectively. FLA encephalitis has been reported across China, but the clinical data descriptions and analytical results of these different reports vary widely. Currently, no consensus treatment has been established. We conduct a systematic review to evaluate the exposure location, clinical symptoms, diagnosis, treatment, and prognosis of three FLA encephalitis and aim to reveal the differences between three FLA encephalitis in China.MethodsWe used MEDLINE (PubMed interface), EMBASE, China National Knowledge Infrastructure (CNKI), Wanfang database, and China Biology Medicine disc (CBMdisc) databases for literatures published and manually retrieve the hospital records of our hospital. The search time was up to August 30, 2022, with no language restrictions.ResultsAfter excluding possible duplicate cases, a total of 48 patients of three FLA encephalitis were collected. One from the medical records of our hospital and 47 patients from 31 different studies. There were 11 patients of PAM, 10 patients of GAE, and 27 patients of BAE. The onset of PAM is mostly acute or subacute, and the clinical symptoms are acute and fulminant hemorrhagic meningoencephalitis. Most patients with GAE and BAE have an insidious onset and a chronic course. A total of 21 BAE patients (77.8%) had skin lesions before onset of symptoms. Additionally, 37 cases (77.1%) were diagnosed with FLA encephalitis before death. And there were 4 of PAM, 2 of GAE, and 10 of BAE diagnosed using next generation sequencing. No single agent can be proposed as the ideal therapy by itself. Only 6 cases were successfully treated.ConclusionsThis review provides an overview of the available data and studies of FLA encephalitis in China and identify some potential differences. FLA encephalitis is a rare but pathogenic infection, and physicians should early identify this encephalitis to improve survival.
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- 2023
39. Senolytic treatment rescues blunted muscle hypertrophy in old mice
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Cory M, Dungan, Vandre C, Figueiredo, Yuan, Wen, Georgia L, VonLehmden, Christopher J, Zdunek, Nicholas T, Thomas, C Brooks, Mobley, Kevin A, Murach, Camille R, Brightwell, Douglas E, Long, Christopher S, Fry, Philip A, Kern, John J, McCarthy, and Charlotte A, Peterson
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Male ,Mice, Inbred C57BL ,Mice ,Aging ,Senotherapeutics ,Animals ,Humans ,Hypertrophy ,Geriatrics and Gerontology ,Muscle, Skeletal - Abstract
With aging, skeletal muscle plasticity is attenuated in response to exercise. Here, we report that senescent cells, identified using senescence-associated β-galactosidase (SA β-Gal) activity and p21 immunohistochemistry, are very infrequent in resting muscle, but emerge approximately 2 weeks after a bout of resistance exercise in humans. We hypothesized that these cells contribute to blunted hypertrophic potential in old age. Using synergist ablation-induced mechanical overload (MOV) of the plantaris muscle to model resistance training in adult (5–6-month) and old (23–24-month) male C57BL/6 J mice, we found increased senescent cells in both age groups during hypertrophy. Consistent with the human data, there were negligible senescent cells in plantaris muscle from adult and old sham controls, but old mice had significantly more senescent cells 7 and 14 days following MOV relative to young. Old mice had blunted whole-muscle hypertrophy when compared to adult mice, along with smaller muscle fibers, specifically glycolytic type 2x + 2b fibers. To ablate senescent cells using a hit-and-run approach, old mice were treated with vehicle or a senolytic cocktail consisting of 5 mg/kg dasatinib and 50 mg/kg quercetin (D + Q) on days 7 and 10 during 14 days of MOV; control mice underwent sham surgery with or without senolytic treatment. Old mice given D + Q had larger muscles and muscle fibers after 14 days of MOV, fewer senescent cells when compared to vehicle-treated old mice, and changes in the expression of genes (i.e., Igf1, Ddit4, Mmp14) that are associated with hypertrophic growth. Our data collectively show that senescent cells emerge in human and mouse skeletal muscle following a hypertrophic stimulus and that D + Q improves muscle growth in old mice.
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- 2022
40. PERK signaling activation restores nucleus pulposus degeneration by activating autophagy under hypoxia environment
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Xiaolong Shen, Chen Yang, Ye Tian, Yang Gao, Yuan Wen, Chen Huajiang, Cao Peng, Chen Xu, Ruizhe Wang, and Huajian Zhong
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musculoskeletal diseases ,Agonist ,Nucleus Pulposus ,medicine.drug_class ,Biomedical Engineering ,Degeneration (medical) ,Mice ,eIF-2 Kinase ,Rheumatology ,Autophagy ,Animals ,Medicine ,Orthopedics and Sports Medicine ,Viability assay ,business.industry ,ATF4 ,Hypoxia (medical) ,musculoskeletal system ,Cell Hypoxia ,Cell biology ,Knockout mouse ,medicine.symptom ,business ,Function (biology) ,Signal Transduction - Abstract
Objectives Intervertebral disc (IVD) degeneration is an important disease with no efficient biological therapy identified. Autophagy, a wildly known therapeutic target for human disease, has been demonstrated to be activated under hypoxia, with underlying mechanism remains elusive. Thus, this study aims to specify the role of autophagy in IVD degeneration, the regulating mechanism of hypoxia-inducing autophagy, and the therapeutic value of autophagy for IVD degeneration. Methods RNA-seq was used to screen the primary pathway affected in NP cells under hypoxia, the specific link between hypoxia and autophagy were investigated using ChIP-seq and dual luciferase reporter assay. Conditional ATG7 knockout mice (ATG7-/-) were constructed for assessing the effect of autophagy on IVD degeneration, and puncture induced mice model of IVD degeneration were used for intradiscal injection to evaluate the therapeutic value of autophagy. Results We demonstrated that hypoxia induces autophagy by transcriptional activation of autophagic gene LC3B and ATG7, which is controlled by PERK signaling. Then, we observed that inhibiting autophagy or PERK signaling leads to impaired NP cell viability and function, furthermore, using ATG7 knockout (ATG7-/-) mice, we identified the protective role of autophagy in IVD. Furthermore, we found that intradiscal injection of PERK signaling agonist, CCT020312, significantly restores the degeneration level of needle punctured mice IVD. Conclusion We showed that the activation of PERK signaling upon hypoxia serves as a vital mechanism to induce autophagy and identified the therapeutic value of PERK signaling agonist for IVD degeneration treatment.
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- 2022
41. Photosynthetic induction of the leaves varies among pepper cultivars due to stomatal oscillation
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Yuan Wen, Yuqi Zhang, Ruifeng Cheng, and Tao Li
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Horticulture - Published
- 2023
42. Straw return counteracts the negative effects of warming on microbial community and soil multifunctionality
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Gong Wu, Jun Ling, De-Qiang Zhao, Zi-Xi Liu, Yi-Ping Xu, Yakov Kuzyakov, Karina Marsden, Yuan Wen, and Shun-Li Zhou
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Ecology ,Animal Science and Zoology ,Agronomy and Crop Science - Published
- 2023
43. Exosome-Derived lncRNA NEAT1 Exacerbates Sepsis-Associated Encephalopathy by Promoting Ferroptosis Through Regulating miR-9-5p/TFRC and GOT1 Axis
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Xue-biao Wei, Wen-qiang Jiang, Ju-hao Zeng, Lin-qiang Huang, Hong-guang Ding, Yuan-wen Jing, Yong-li Han, Yi-chen Li, and Sheng-long Chen
- Subjects
MicroRNAs ,Cellular and Molecular Neuroscience ,Neurology ,Sepsis-Associated Encephalopathy ,Receptors, Transferrin ,Neuroscience (miscellaneous) ,Animals ,Ferroptosis ,RNA, Long Noncoding ,Exosomes ,Rats - Abstract
Sepsis can cause sepsis-associated encephalopathy (SAE), but whether SAE was induced or exacerbated by ferroptosis remains unknown. In this study, the rat sepsis model was constructed using the cecal ligation and puncture method. The blood–brain barrier (BBB) permeability was measured by Evans blue dye (EBD) in vivo. The levels of ROS, Fe ion, MDA, GSH, and GPX4 were assessed by enzyme-linked immunosorbent assay (ELISA). The exosomes isolated from serum were cultured with bEnd.3 cells for the in vitro analysis. Moreover, bEnd.3 cells cultured with 100 μM FeCl3 (iron-rich) were to simulate ferroptosis stress. The cell viability was evaluated by Cell Counting Kit-8 (CCK-8) assay. A dual-luciferase reporter gene assay was performed to confirm the relationship between miR-9-5p with NEAT1, TFRC, and GOT1. In vivo, it is found that BBB permeability was damaged in model rats. Level of ROS, Fe ion, and MDA was increased, and level of GSH and GPX4 was decreased, which means ferroptosis was induced by sepsis. Exosome-packaged NEAT1 in serum was significantly upregulated in model rats. In vitro, it is found that NEAT1 functions as a ceRNA for miR-9-5p to facilitate TFRC and GOT1 expression. Overexpression of NEAT1 enhanced ferroptosis stress in bEnd.3 cells. Increased miR-9-5p alleviated sepsis-induced ferroptosis by suppressing the expression of TFRC and GOT1 both in vivo and in vitro. In conclusion, these findings suggest that sepsis induced high expression of serous exosome-derived NEAT1, and it might exacerbate SAE by promoting ferroptosis through regulating miR-9-5p/TFRC and GOT1 axis.
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- 2022
44. Epigenetic evidence for distinct contributions of resident and acquired myonuclei during long-term exercise adaptation using timed in vivo myonuclear labeling
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Yuan Wen, Kevin A. Murach, Ferdinand von Walden, and Cory M. Dungan
- Subjects
Male ,Time Factors ,Satellite Cells, Skeletal Muscle ,Physiology ,Green Fluorescent Proteins ,Muscle Fibers, Skeletal ,Mice, Transgenic ,Biology ,Epigenesis, Genetic ,Mice ,Physical Conditioning, Animal ,Animals ,Epigenetics ,Transcription factor ,Cell Nucleus ,Staining and Labeling ,Rapid Report ,Protein turnover ,Promoter ,Cell Biology ,Methylation ,biology.organism_classification ,Adaptation, Physiological ,Cell biology ,DNA methylation ,Satellite (biology) ,Stem cell - Abstract
Muscle fibers are syncytial postmitotic cells that can acquire exogenous nuclei from resident muscle stem cells, called satellite cells. Myonuclei are added to muscle fibers by satellite cells during conditions such as load-induced hypertrophy. It is difficult to dissect the molecular contributions of resident versus satellite cell-derived myonuclei during adaptation due to the complexity of labeling distinct nuclear populations in multinuclear cells without label transference between nuclei. To sidestep this barrier, we used a genetic mouse model where myonuclear DNA can be specifically and stably labeled via nonconstitutive H2B-GFP at any point in the lifespan. Resident myonuclei (Mn) were GFP-tagged in vivo before 8 wk of progressive weighted wheel running (PoWeR) in adult mice (>4-mo-old). Resident + satellite cell-derived myonuclei (Mn+SC Mn) were labeled at the end of PoWeR in a separate cohort. Following myonuclear isolation, promoter DNA methylation profiles acquired with low-input reduced representation bisulfite sequencing (RRBS) were compared to deduce epigenetic contributions of satellite cell-derived myonuclei during adaptation. Resident myonuclear DNA has hypomethylated promoters in genes related to protein turnover, whereas the addition of satellite cell-derived myonuclei shifts myonuclear methylation profiles to favor transcription factor regulation and cell-cell signaling. By comparing myonucleus-specific methylation profiling to previously published single-nucleus transcriptional analysis in the absence (Mn) versus the presence of satellite cells (Mn+SC Mn) with PoWeR, we provide evidence that satellite cell-derived myonuclei may preferentially supply specific ribosomal proteins to growing myofibers and retain an epigenetic “memory” of prior stem cell identity. These data offer insights on distinct epigenetic myonuclear characteristics and contributions during adult muscle growth.
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- 2022
45. Analytical Placement with 3D Poisson’s Equation and ADMM Based Optimization for Large-Scale 2.5D Heterogeneous FPGAs
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Min Wei, Xingyu Tong, Yuan Wen, Jianli Chen, Jun Yu, Wenxing Zhu, and Yao-Wen Chang
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Electrical and Electronic Engineering ,Computer Graphics and Computer-Aided Design ,Computer Science Applications - Abstract
As the design complexity keeps increasing, the 2.5D FPGA with large logic capacity has become popular in modern circuit applications. A 2.5D FPGA consists of multiple dies connected through super long lines (SLLs) on an interposer. Each die contains heterogeneous logic blocks and ASIC-like clocking architectures to achieve better skew and timing. Existing works consider these problems separately and thus may lead to serious timing issues or routing failure. This paper presents an analytical placement algorithm for the 2.5D FPGA to simultaneously minimize the number of inter-die SLL signals and intra-die clocking violations. Using a lifting dimension technique, we first formulate the 2.5D global placement problem as a three-dimensional continuous and differential minimization problem, where the SLL-aware block distribution is modeled by 3D Poisson’s equation and directly solved to obtain an analytical solution. Then, we further reformulate the minimization problem as a separable optimization problem with linear constraints. Based on the proximal alternating direction method of multipliers (ADMM) optimization method, we efficiently optimize the separable subproblems one by one in an alternating fashion. Finally, clock-aware legalization and detailed placement are applied to legalize and improve our placement results. Compared with the state-of-the-art works, experimental results show that our algorithm can resolve all clocking constraints and reduce the number of SLL crossing signals by 36.9% with similar wirelength in a comparable running time.
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- 2023
46. A case report of autoimmune GFAP astrocytopathy presenting with abnormal heart rate variability and blood pressure variability
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Pu-yuan Wen, Guang-qiang Wang, Lian-wei Dou, Qi Chen, Xian-wen Chen, and Li Gong
- Subjects
Neurology (clinical) ,General Medicine - Abstract
Background Autonomic dysfunctions including bladder dysfunction, gastrointestinal dysfunction and orthostasis are common symptoms of autoimmune glial fibrillary acidic protein astrocytopathy (A-GFAP-A); however, cardiac autonomic dysfunction and abnormal circadian rhythm of blood pressure, which can lead to poor prognosis and even sudden cardiac death, has never been reported in A-GFAP-A patient. Case presentation A 68-year-old male Chinese patient presented to our hospital with headache, fever, progressive disturbance of consciousness, dysuria, and limb weakness. Abnormal heart rate variability and non-dipper circadian rhythm of blood pressure gradually developed during hospitalization, which is rare in A-GFAP-A. He had positive GFAP IgG in cerebrospinal fluid (CSF). Enhanced brian MRI showed uneven enhancement and T2 hyperintense lesions of medulla oblongata; Cervical spine MRI showed T2 hyperintense lesions in medulla oblongata and upper margin of the T2 vertebral body. A contrast-enhanced thoracic spine MRI showed uneven enhancement and T2 hyperintense lesions of T1 to T6 vertebral segments. After treatment with intravenous immunoglobulin and corticosteroids, the patient’s symptoms, including autonomic dysfunction, alleviated dramatically. Finally, his heart rate variability and blood pressure variability became normal. Conclusions Our case broadens the spectrum of expected symptoms in A-GFAP- A syndromes as it presented with heart rate variability and blood pressure variability.
- Published
- 2023
47. Anterior cervical discectomy and fusion, open-door laminoplasty, or laminectomy with fusion: Which is the better treatment for four-level cervical spondylotic myelopathy?
- Author
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Zhong, Huajian, Xu, Chen, Wang, Ruizhe, Wu, Xiaodong, Wu, Huiqiao, Sun, Baifeng, Wang, Xinwei, Chen, Huajiang, Shen, Xiaolong, and Yuan, Wen
- Subjects
Surgery - Abstract
Four-level cervical spondylotic myelopathy (CSM) is a common disease affecting a large number of people, with the optimal surgical strategy remaining controversial. This study compared the clinical outcomes, radiological parameters, and postoperative complications of primarily performed surgical procedures such as anterior cervical discectomy and fusion (ACDF), open-door laminoplasty (LAMP), and laminectomy with fusion (LF) in treating four-level CSM. A total of 116 patients who received ACDF (38 cases), LAMP (45 cases), and LF (33 cases) were followed up for a minimum of 24 months were enrolled in this study and retrospectively analyzed. Clinical outcomes were evaluated using the Japanese Orthopedic Association (JOA) scoring system, the Neck Disability Index (NDI), and the Visual Analogue Scale (VAS). Changes in the curvature of the cervical spine were determined using the cervical curvature index (CCI) and the C2–C7 Cobb angle. Cervical mobility was evaluated using the C2–C7 range of motion (ROM) and active cervical ROM (aROM). Complications were recorded and compared among the three groups. All patients achieved significant improvement in JOA, NDI, and VAS scores at the final follow-up (P P > 0.05). In addition, both C2–7 ROM and aROM were significantly reduced in the three groups and LAMP showed the least reduction relatively. As for complications, LAMP showed the lowest overall incidence of postoperative complications, and patients in the ACDF group were more susceptible to dysphagia, pseudoarthrosis than LAMP and LF. Considering improvements in clinical symptoms and neurological function, no remarkable difference was found among the groups. Nevertheless, LAMP had advantages over the other two surgical procedures in terms of preserving cervical mobility and reducing postoperative complications.
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- 2023
48. Associations between maternal diet during pregnancy and fatty acids composition in maternal blood, cord blood and colostrum samples
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YUAN, Wen, Y, Jonathan, Armand, Martine, Charles, Marie, and Heude, Barbara
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Environmental Public Health ,Maternal and Child Health ,colostrum ,dairy products consumption ,Medicine and Health Sciences ,cord blood ,Public Health ,EDEN study ,fatty acids ,mother-child cohort ,Dietetics and Clinical Nutrition ,maternal blood - Abstract
Previous evidence suggested that maternal diet might influence her child development through placental exchanges and breastfeeding. Active transfer of fatty acids through placenta, e.g. polyunsaturated fatty acids, are of great interest because of their implications in the child’s brain development. There is a lack of evidence regarding the influence of maternal dairy consumption during pregnancy on the fatty acids’ composition of blood cells membrane in maternal or cord blood and colostrum.
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- 2023
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49. Brezis--Seeger--Van Schaftingen--Yung-Type Characterization of Homogeneous Ball Banach Sobolev Spaces and Its Applications
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Zhu, Chenfeng, Yang, Dachun, and Yuan, Wen
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Mathematics - Functional Analysis ,Mathematics - Analysis of PDEs ,Mathematics - Classical Analysis and ODEs ,Classical Analysis and ODEs (math.CA) ,FOS: Mathematics ,Primary 46E35, Secondary 35A23, 42B25, 26D10 ,Functional Analysis (math.FA) ,Analysis of PDEs (math.AP) - Abstract
Let $\gamma\in\mathbb{R}\setminus\{0\}$ and $X(\mathbb{R}^n)$ be a ball Banach function space satisfying some mild assumptions. Assume that $\Omega=\mathbb{R}^n$ or $\Omega\subset\mathbb{R}^n$ is an $(\varepsilon,\infty)$-domain for some $\varepsilon\in(0,1]$. In this article, the authors prove that a function $f$ belongs to the homogeneous ball Banach Sobolev space $\dot{W}^{1,X}(\Omega)$ if and only if $f\in L_{\mathrm{loc}}^1(\Omega)$ and $$ \sup_{\lambda\in(0,\infty)}\lambda \left\|\left[\int_{\{y\in\Omega:\ |f(\cdot)-f(y)|>\lambda|\cdot-y|^{1+\frac{\gamma}{p}}\}} \left|\cdot-y\right|^{\gamma-n}\,dy \right]^\frac{1}{p}\right\|_{X(\Omega)}, Comment: arXiv admin note: text overlap with arXiv:2304.00949
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- 2023
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50. Long-Range Delivery and High-Efficiency Actuation of Magnetic Freezing Compact Toroid for Failed Spacecraft Despinning
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Ma Tian, Zhao Hong-Liang, Chen Peng-Lin, Zhang Yuan-Wen, and Huang Huan
- Published
- 2023
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