1. Pulmonary resident neutrophils regulate the production of GM-CSF and alveolar macrophages
- Author
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Xie Nianlin, Xiaolong Yan, Jie Lei, Feng Tian, Yongan Zhou, Daixing Zhong, Jinbo Zhao, Xiaofei Li, Jian Song, Jian Wang, Yong Han, Liang Xiaohua, and Xiaoping Wang
- Subjects
0301 basic medicine ,Neutrophils ,Population ,Blotting, Western ,CD18 ,Enzyme-Linked Immunosorbent Assay ,Granulocyte ,Biology ,Real-Time Polymerase Chain Reaction ,Biochemistry ,Granulopoiesis ,03 medical and health sciences ,Mice ,Macrophages, Alveolar ,medicine ,Macrophage ,Animals ,RNA, Messenger ,education ,Molecular Biology ,Lung ,Cells, Cultured ,Mice, Knockout ,education.field_of_study ,Reverse Transcriptase Polymerase Chain Reaction ,Granulocyte-Macrophage Colony-Stimulating Factor ,Cell Differentiation ,Cell Biology ,respiratory system ,Flow Cytometry ,Mice, Inbred C57BL ,030104 developmental biology ,medicine.anatomical_structure ,Granulocyte macrophage colony-stimulating factor ,CD18 Antigens ,Immunology ,Alveolar macrophage ,Cytokines ,medicine.drug - Abstract
Alveolar macrophages exist in the lung airspaces, and their differentiation and function are considerably regulated by the microenvironment. In this study, we examine the important role of resident neutrophil/IL-23/granulocyte/macrophage colony-stimulating factor (GM-CSF) axis in the development and preferential phenotype of alveolar macrophages under physiological conditions. Using CD18-deficient (CD18(-/-) ) mice, we show a correlation between increased granulopoiesis and enhanced alveolar macrophage development in an IL-23- and GM-CSF-dependent manner. The apoptotic neutrophils could inhibit the secretion of IL-23 from alveolar macrophages, which is important for the production of GM-CSF, and depletion of neutrophils disrupted the regulation of IL-23 and GM-CSF. This study reveals a mechanism for the regulation of the local alveolar macrophage population and function by neutrophil apoptosis in the circulatory system.
- Published
- 2015