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Pulmonary resident neutrophils regulate the production of GM-CSF and alveolar macrophages
- Source :
- The FEBS journal. 283(8)
- Publication Year :
- 2015
-
Abstract
- Alveolar macrophages exist in the lung airspaces, and their differentiation and function are considerably regulated by the microenvironment. In this study, we examine the important role of resident neutrophil/IL-23/granulocyte/macrophage colony-stimulating factor (GM-CSF) axis in the development and preferential phenotype of alveolar macrophages under physiological conditions. Using CD18-deficient (CD18(-/-) ) mice, we show a correlation between increased granulopoiesis and enhanced alveolar macrophage development in an IL-23- and GM-CSF-dependent manner. The apoptotic neutrophils could inhibit the secretion of IL-23 from alveolar macrophages, which is important for the production of GM-CSF, and depletion of neutrophils disrupted the regulation of IL-23 and GM-CSF. This study reveals a mechanism for the regulation of the local alveolar macrophage population and function by neutrophil apoptosis in the circulatory system.
- Subjects :
- 0301 basic medicine
Neutrophils
Population
Blotting, Western
CD18
Enzyme-Linked Immunosorbent Assay
Granulocyte
Biology
Real-Time Polymerase Chain Reaction
Biochemistry
Granulopoiesis
03 medical and health sciences
Mice
Macrophages, Alveolar
medicine
Macrophage
Animals
RNA, Messenger
education
Molecular Biology
Lung
Cells, Cultured
Mice, Knockout
education.field_of_study
Reverse Transcriptase Polymerase Chain Reaction
Granulocyte-Macrophage Colony-Stimulating Factor
Cell Differentiation
Cell Biology
respiratory system
Flow Cytometry
Mice, Inbred C57BL
030104 developmental biology
medicine.anatomical_structure
Granulocyte macrophage colony-stimulating factor
CD18 Antigens
Immunology
Alveolar macrophage
Cytokines
medicine.drug
Subjects
Details
- ISSN :
- 17424658
- Volume :
- 283
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- The FEBS journal
- Accession number :
- edsair.doi.dedup.....bf3af35a60d8bab95e39902ad970e017