3 results on '"Verfaillie, Catherine M."'
Search Results
2. G(i)-coupled GPCR signaling controls the formation and organization of human pluripotent colonies
- Author
-
Kenta Nakamura, Kiichiro Tomoda, Nathan Salomonis, Bruce R. Conklin, Shinya Yamanaka, and Verfaillie, Catherine M
- Subjects
Somatic cell ,medicine.medical_treatment ,lcsh:Medicine ,Apoptosis ,GTP-Binding Protein alpha Subunits, Gi-Go ,medicine.disease_cause ,Regenerative Medicine ,Gi-Go ,Cell Biology/Cell Signaling ,Bordetella pertussis ,Receptors, G-Protein-Coupled ,Developmental Biology/Molecular Development ,Mice ,0302 clinical medicine ,Models ,Receptors ,Developmental Biology/Developmental Molecular Mechanisms ,lcsh:Science ,Induced pluripotent stem cell ,Vibrio cholerae ,0303 health sciences ,Multidisciplinary ,Developmental Biology/Morphogenesis and Cell Biology ,Cholera toxin ,Stem-cell therapy ,GTP-Binding Protein alpha Subunits ,Developmental Biology/Stem Cells ,Infectious Diseases ,Stem cell ,Reprogramming ,Research Article ,Signal Transduction ,Pluripotent Stem Cells ,Cholera Toxin ,General Science & Technology ,Cell Biology/Developmental Molecular Mechanisms ,Biology ,Pertussis toxin ,Models, Biological ,03 medical and health sciences ,G-Protein-Coupled ,medicine ,Animals ,Humans ,Stem Cell Research - Embryonic - Human ,Embryonic Stem Cells ,030304 developmental biology ,Cell Proliferation ,lcsh:R ,Biological ,Stem Cell Research ,Molecular biology ,Embryonic stem cell ,Pertussis Toxin ,lcsh:Q ,030217 neurology & neurosurgery - Abstract
Author(s): Nakamura, Kenta; Salomonis, Nathan; Tomoda, Kiichiro; Yamanaka, Shinya; Conklin, Bruce R | Abstract: BackgroundReprogramming adult human somatic cells to create human induced pluripotent stem (hiPS) cell colonies involves a dramatic morphological and organizational transition. These colonies are morphologically indistinguishable from those of pluripotent human embryonic stem (hES) cells. G protein-coupled receptors (GPCRs) are required in diverse developmental processes, but their role in pluripotent colony morphology and organization is unknown. We tested the hypothesis that G(i)-coupled GPCR signaling contributes to the characteristic morphology and organization of human pluripotent colonies.Methodology/principal findingsSpecific and irreversible inhibition of G(i)-coupled GPCR signaling by pertussis toxin markedly altered pluripotent colony morphology. Wild-type hES and hiPS cells formed monolayer colonies, but colonies treated with pertussis toxin retracted inward, adopting a dense, multi-layered conformation. The treated colonies were unable to reform after a scratch wound insult, whereas control colonies healed completely within 48 h. In contrast, activation of an alternative GPCR pathway, G(s)-coupled signaling, with cholera toxin did not affect colony morphology or the healing response. Pertussis toxin did not alter the proliferation, apoptosis or pluripotency of pluripotent stem cells.Conclusions/significanceExperiments with pertussis toxin suggest that G(i) signaling plays a critical role in the morphology and organization of pluripotent colonies. These results may be explained by a G(i)-mediated density-sensing mechanism that propels the cells radially outward. GPCRs are a promising target for modulating the formation and organization of hiPS and hES cell colonies and may be important for understanding somatic cell reprogramming and for engineering pluripotent stem cells for therapeutic applications.
- Published
- 2009
3. Optimization of Multimodal Imaging of Mesenchymal Stem Cells Using the Human Sodium Iodide Symporter for PET and Cerenkov Luminescence Imaging
- Author
-
Esther Wolfs, Koen Van Laere, Catherine M. Verfaillie, Zeger Debyser, Rik Gijsbers, Cindy Casteels, Michael Maris, Scott J. Roberts, Christophe Deroose, Bryan Holvoet, Tom Struys, Abdelilah Ibrahimi, Rameshwar, Pranela, WOLFS, Esther, Holvoet, Bryan, Gijsbers, Rik, Casteels, Cindy, Roberts, Scott J., STRUYS, Tom, Maris, Michael, Ibrahimi, Abdelilah, Debyser, Zeger, Van Laere, Koen, Verfaillie, Catherine M., and Deroose, Christophe M.
- Subjects
Pathology ,Luminescence ,medicine.medical_treatment ,Multimodal Imaging ,Diagnostic Radiology ,Iodine Radioisotopes ,Mice ,Peptide Elongation Factor 1 ,Animal Cells ,Genes, Reporter ,Molecular Cell Biology ,Medicine and Health Sciences ,Tomography ,Multidisciplinary ,Symporters ,Chemistry ,Stem Cells ,Radiology and Imaging ,Optical Imaging ,Cell Differentiation ,Stem-cell therapy ,Adult Stem Cells ,Medicine ,Puromycin ,Cellular Types ,Viral Vectors ,Preclinical imaging ,Research Article ,Sodium-iodide symporter ,medicine.medical_specialty ,Science ,Genetic Vectors ,Microbiology ,Diagnostic Medicine ,Virology ,medicine ,Animals ,Humans ,Bioluminescence imaging ,Luciferase ,Radionuclide Imaging ,Reporter gene ,Lentivirus ,Mesenchymal stem cell ,Biology and Life Sciences ,Mesenchymal Stem Cells ,Cell Biology ,HEK293 Cells ,Positron-Emission Tomography ,Symporter ,Biophysics ,Viral Transmission and Infection ,Positron Emission Tomography ,Developmental Biology - Abstract
PURPOSE: The use of stably integrated reporter gene imaging provides a manner to monitor the in vivo fate of engrafted cells over time in a non-invasive manner. Here, we optimized multimodal imaging (small-animal PET, Cerenkov luminescence imaging (CLI) and bioluminescence imaging (BLI)) of mesenchymal stem cells (MSCs), by means of the human sodium iodide symporter (hNIS) and firefly luciferase (Fluc) as reporters. METHODS: First, two multicistronic lentiviral vectors (LV) were generated for multimodal imaging: BLI, 124I PET/SPECT and CLI. Expression of the imaging reporter genes was validated in vitro using 99mTcO4- radioligand uptake experiments and BLI. Uptake kinetics, specificity and tracer elution were determined as well as the effect of the transduction process on the cell's differentiation capacity. MSCs expressing the LV were injected intravenously or subcutaneously and imaged using small-animal PET, CLI and BLI. RESULTS: The expression of both imaging reporter genes was functional and specific. An elution of 99mTcO4- from the cells was observed, with 31% retention after 3 h. After labeling cells with 124I in vitro, a significantly higher CLI signal was noted in hNIS expressing murine MSCs. Furthermore, it was possible to visualize cells injected intravenously using BLI or subcutaneously in mice, using 124I small-animal PET, CLI and BLI. CONCLUSIONS: This study identifies hNIS as a suitable reporter gene for molecular imaging with PET and CLI, as confirmed with BLI through the expression of Fluc. It supports the potential for a wider application of hNIS reporter gene imaging and future clinical applications. ispartof: PLOS ONE vol:9 issue:4 ispartof: location:United States status: published
- Published
- 2014
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.