Back to Search
Start Over
Optimization of Multimodal Imaging of Mesenchymal Stem Cells Using the Human Sodium Iodide Symporter for PET and Cerenkov Luminescence Imaging
- Source :
- PLoS ONE, Vol 9, Iss 4, p e94833 (2014), PLoS ONE
- Publication Year :
- 2014
- Publisher :
- Public Library of Science (PLoS), 2014.
-
Abstract
- PURPOSE: The use of stably integrated reporter gene imaging provides a manner to monitor the in vivo fate of engrafted cells over time in a non-invasive manner. Here, we optimized multimodal imaging (small-animal PET, Cerenkov luminescence imaging (CLI) and bioluminescence imaging (BLI)) of mesenchymal stem cells (MSCs), by means of the human sodium iodide symporter (hNIS) and firefly luciferase (Fluc) as reporters. METHODS: First, two multicistronic lentiviral vectors (LV) were generated for multimodal imaging: BLI, 124I PET/SPECT and CLI. Expression of the imaging reporter genes was validated in vitro using 99mTcO4- radioligand uptake experiments and BLI. Uptake kinetics, specificity and tracer elution were determined as well as the effect of the transduction process on the cell's differentiation capacity. MSCs expressing the LV were injected intravenously or subcutaneously and imaged using small-animal PET, CLI and BLI. RESULTS: The expression of both imaging reporter genes was functional and specific. An elution of 99mTcO4- from the cells was observed, with 31% retention after 3 h. After labeling cells with 124I in vitro, a significantly higher CLI signal was noted in hNIS expressing murine MSCs. Furthermore, it was possible to visualize cells injected intravenously using BLI or subcutaneously in mice, using 124I small-animal PET, CLI and BLI. CONCLUSIONS: This study identifies hNIS as a suitable reporter gene for molecular imaging with PET and CLI, as confirmed with BLI through the expression of Fluc. It supports the potential for a wider application of hNIS reporter gene imaging and future clinical applications. ispartof: PLOS ONE vol:9 issue:4 ispartof: location:United States status: published
- Subjects :
- Pathology
Luminescence
medicine.medical_treatment
Multimodal Imaging
Diagnostic Radiology
Iodine Radioisotopes
Mice
Peptide Elongation Factor 1
Animal Cells
Genes, Reporter
Molecular Cell Biology
Medicine and Health Sciences
Tomography
Multidisciplinary
Symporters
Chemistry
Stem Cells
Radiology and Imaging
Optical Imaging
Cell Differentiation
Stem-cell therapy
Adult Stem Cells
Medicine
Puromycin
Cellular Types
Viral Vectors
Preclinical imaging
Research Article
Sodium-iodide symporter
medicine.medical_specialty
Science
Genetic Vectors
Microbiology
Diagnostic Medicine
Virology
medicine
Animals
Humans
Bioluminescence imaging
Luciferase
Radionuclide Imaging
Reporter gene
Lentivirus
Mesenchymal stem cell
Biology and Life Sciences
Mesenchymal Stem Cells
Cell Biology
HEK293 Cells
Positron-Emission Tomography
Symporter
Biophysics
Viral Transmission and Infection
Positron Emission Tomography
Developmental Biology
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....cd1ba1076e2b71274e6dacf611dc448a