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Jabuticaba is a Brazilian berry known for its therapeutic potential against cancer, obesity, insulin resistance (IR), and others. It is a natural source of bioactive compounds, leading to better glucose metabolism, and attenuating obesity and IR through the reduction of pro-inflammatory status. The present study aimed to observe the prebiotic effect of freeze-dried jabuticaba peel (J) consumption on gut bacteria profile and describe its effects on IR derived from the lipopolysaccharides/Toll-like receptor-4 inflammatory pathway. Jabuticaba peel was chemically characterized, and its bioactive compounds were quantified. Twenty-four C57BL/6 mice were feed with a control diet (n = 6), control diet + J (n = 6), high-fat diet (HF) (n = 6), and HF + J (n = 6) for thirteen weeks. Gut bacteriota (16s RNA sequencing), glucose metabolism (fasting glucose and insulin, OGTT, ITT, HOMA-IR, and β, QUICKI), and inflammatory status (serum lipopolysaccharide, and protein expression) were assessed. The main bioactive compounds found in J were dietary fiber, and anthocyanins, and its consumption along with a healthy diet reduced the abundance of Firmicutes and Actinobacteriota phyla (p

In this study, the effects of an agro-industrial residue with active properties, pomegranate peel extract (PPE), were evaluated on the rheological properties of potential coatings based on chitosan (C) and gelatin (G). For this, rheological properties of the polymeric solutions were investigated in relation to PPE concentration (2 or 4 mg PPE g−1 solution), and to its incorporation order into the system (in C or in CG mixture). All solutions were more viscous than elastic (G″ &gt, G′), and the change in PPE concentration had a greater influence accentuating the viscous character of the samples in which PPE was added to the CG mixture (CGPPE2 and CGPPE4). PPE addition to the CG mixture increased the angular frequency at the moduli crossover, indicating the formation of a more resistant polymeric network. This tendency was also observed in flow results, in which PPE addition decreased the pseudoplastic behavior of the solutions, due to a greater cross-linking between the polymers and the phenolic compounds. In general, all the studied solutions showed viscosities suitable for the proposed application, and it was possible to state the importance of standardizing the addition order of the components during the preparation of a coating.

Made available in DSpace on 2021-06-25T10:19:11Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-04-01 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Strawberry has a short postharvest shelf life, mainly because of the gray mold caused by Botrytis cinerea. The chemical composition and antifungal activities of essential oils from Mentha piperita, Cymbopogon martinii, Cinnamomum camphora, Mentha spicata, and their binary mixtures against B. cinerea were evaluated by different in vitro methods, and also in vivo in vapor-phase on inoculated strawberries. The major components present in M. piperita essential oil were menthol (45.37%), menthone (20.13%), isomenthone (16.94%), menthyl acetate (3.81%), pulegone (1.89%), α-terpinene (1.88%), isopulegol (1.83%), neoisomenthol (1.19%), and α-terpineol (1.08%), in C. martinii essential oil were geraniol (83.82%), geranyl acetate (7.49%), linalool (2.48%), and caryophyllene (1.33%), in C. camphora essential oil were linalool (98.39%), and M. spicata essential oil were carvone (61.71%), limonene (20.22%), 1,8-cineole (5%), sabinene (2.28%), cis-dihydrocarvone (1.63%), and α-thujene (1.4%). The highest antifungal activity in vitro was presented by M. spicata and C. martinii essential oils by the direct contact, vapor contact, spore germination, and micro-well dilution methods. In vivo, strawberries treated with the vapor of M. spicata and C. martinii essential oils showed a reduction in disease incidence and severity. At a 10% concentration, M. spicata and C. martinii showed a reduction of 100% and 83% in the disease incidence, respectively. Thus, the M. spicata and C. martinii essential oils may be potential alternatives for the control of B. cinerea in postharvest fresh strawberries. School of Pharmaceutical Sciences São Paulo State University (UNESP), Rod. Araraquara-Jaú Km 1 - Machados Federal University of São Carlos Federal University of Paraná Brazilian Agricultural Research Corporation Embrapa Agroindústria Tropical São Carlos Institute of Chemistry (IQSC) University of São Paulo (USP) Brazilian Agricultural Research Corporation Embrapa Instrumentação School of Pharmaceutical Sciences São Paulo State University (UNESP), Rod. Araraquara-Jaú Km 1 - Machados CNPq: 302381/2016-3 CNPq: 310728/2019-3 FAPESP: process 2018/24612-9

This study reports, for the first time, the chemical composition and biological activity of essential oil from Brazilian Cymbopogon densiflorus flowers. Thirty chemical compounds were identified by gas chromatography coupled with mass spectrometry (GC/MS), corresponding to 95 % of total compounds from an essential oil chromatogram obtained by gas chromatography-flame ionization detection (GC-FID ). The essential oil was mainly composed of cis-p-mentha-1(7),8-dien-2-ol (26 %), trans-p-mentha-1(7),8-dien-2-ol (18 %), trans-p-mentha-2,8-dien-1-ol (13 %) limonene (9 %), cis-p-mentha-2,8-dien-1-ol (7 %), cis-carveol (7 %) and carvone (5 %). The identity confirmation of the major compounds was performed by 1H/13C heteronuclear single-quantum coherence nuclear magnetic resonance (HSQC NMR). The essential oil exhibited a weak antioxidant activity by the DPPH method and a wide spectrum of antimicrobial activity against the pathogenic bacterial strains tested, showing some efficacy against 7 of the 10 strains with the greatest inhibition observed with the Gram-negative bacterium Shigella sonnei.

This study investigated the effects of bio-nanocomposite coatings developed using arrowroot starch (AA), cellulose nanocrystals (CNC), carnauba wax nanoemulsion (CWN), and Cymbopogon martinii and Mentha spicata essential oils (CEO and MEO, respectively) on the physicochemical, microbiological, bioactive, antioxidant, and aromatic characteristics of strawberries cv. 'Oso Grande' in refrigerated storage for 12 days. The coatings improved the shelf life and stability of strawberries, minimizing their weight loss (2.6-3.9 %), as well as changes in color and texture (except for those coated with CEO), titratable acidity, pH, soluble solids, anthocyanins, phenolic compounds, ascorbic acid content, and antioxidant activity compared with uncoated control strawberries. The bio-nanocomposite coatings containing MEO and CEO also exhibited antimicrobial activity, reduced visible fungal deterioration (40-60 %), and reduced microbial load (3.59-4.03 log CFU g

Betalains are natural nitrogenous water-soluble pigments found in species belonging to the Caryophyllales order and in mushrooms. Betalains can be considered multifunctional molecules due to their diverse bioactivities such as antioxidant, antimicrobial, anticancer, and anti-inflammatory. Furthermore, they can detect pH variations in foods and are considered promising colorimetric bioindicators. The bioactivities of betalains have improved their use as active and bioactive agents, and colorimetric indicators in the development of edible and biodegradable films for foods, which are trends in the food packaging market. Thus, this review presents the state-of-art information on the use of betalains as a multifunctional molecule in the development of smart, active, and bioactive edible and biodegradable packaging for foods. Studies have revealed that betalains can be successfully used to develop: smart films to indicate the freshness and spoilage of foods such as shrimp, fish, and chicken; active films with antimicrobial and antioxidant potentials to increase the shelf life of sausage and shrimp; and bioactive films with health benefits.

The internal breakdown (IB) is a premature and uneven mango pulp ripening physiological disorder that is noticed only when the fruit is sliced for consumption. Thus, there is a demand for analytical methods to detect IB in mangoes to avoid consumer dissatisfaction and reduce postharvest waste. In this work, physicochemical and volatile compounds were determined to evaluate the ability to predict pulp IB. Principal components analysis (PCA) and partial least squares discriminant analysis (PLS-DA) of the data show that color, firmness, and volatiles compounds are important to give some information about the physiological changes caused by IB. The volatile compounds methacrylic acid, ethyl ester, isopentyl ethanoate, limonene oxide, (E)-2-pentenal, tetradecane, and γ-elemene were identified as chemical markers of IB. Therefore, mango physical and chemical characteristics combined with PCA and PLS-DA were successfully employed for the identification of IB in mangoes, showing significant differences between healthy and IB fruits.

In this study, chitosan and collagen (Ch: Col)-based materials containing curcumin (Cur) as a bioactive compound were developed for wound-healing purposes. The effects of incorporating curcumin and increasing its concentration on both the rheological properties of the formed solutions and the morphological and thermal properties of the three-dimensional scaffolds obtained from them were evaluated. Rheology showed that the presence of curcumin resulted in solutions with a solid-like behavior (G’ > G″), higher collagen denaturation temperatures, and higher viscosities, favoring their use as biomaterials for wound healing. A greater cross-linking effect was observed at higher curcumin concentrations, possibly between the amino groups from both polymers and the hydroxyl and keto groups from the polyphenol. Such cross-linking was responsible for the delay in the onset of degradation of the scaffolds by 5 °C, as revealed by thermogravimetric analysis. Moreover, the pore diameter distribution profile of the scaffolds changed with increasing curcumin concentration; a greater number of pores with diameters between 40 and 60 µm was observed for the scaffold with the highest curcumin content (50 mg), which would be the most suitable for the proposed application. Thus, the materials developed in this study are presented as promising biomaterials for their biological evaluation in tissue regeneration.

New technologies for food processing have been used to enhance the beneficial effects of foods. This study aimed to evaluate the effects of a prebiotic soursop whey beverage processed by high-intensity ultrasound (HIUS) on healthy rats. Whey beverages were processed by HIUS (20KHz, 520 W of nominal power,53 °C, 20.3 W of acoustic power, energy density of 2.9 kJ.cm

The yield and the composition of the peel essential oils (EO) of six new citrus hybrids obtained from the crosses between Murcott tangor—TM [Citrus reticulata Blanco x Citrus sinensis (L.) ...

In this study, a preventive model was proposed to check whether the intake of Myrciaria jaboticaba berry peel (MJP) could avoid harmful effects caused by a high-fat diet. The intake of the high-fat diet supplemented with MJP (HM mice) down-regulated pro-inflammatory cytokines in adipose tissue and prevented adipose tissue growth and accumulation. In addition, the intake of the high-fat diet supplemented with MJP prevented weight gain, increased the excretion of triglycerides, reduced hepatic steatosis area and stimulated the production of short chain fatty acids (SCFA) by the large intestinal microbiota. Furthermore, hepatic mRNA PPAR-α level was lowered in non-fasted animals of the HM mice, indicating lower oxidation of both fatty acids and lipotoxic metabolites by the liver. In conclusion, MJP intake induces higher production of the gut SCFA, compounds known to counteract obesity markers, as shown by the lowering of adipose tissue inflammation, weight gain, dyslipidemia and hepatic steatosis.

This study hypothesized that polyphenol- and fiber-rich Brazilian fruits could elicit preventive effects in inflammatory bowel diseases (IBDs), even when applied to obese subjects. Therefore, the purpose was to investigate the implications of the consumption of acai pulp and jaboticaba peel on obese mice with dextran sodium sulfate (DSS)-induced colitis. First, obesity was induced by a high-fat diet for 13 weeks, followed by the inclusion of DSS in the water for five days. Animals received during the whole experiment a standard or high-fat diet, with the last one containing or not 5% (w/w) freeze-dried acai or jaboticaba. The diets added with the fruits had a higher content in total phenolic compounds (1.1 mg GAE/g) and an increased antioxidant capacity (up to 25.32 μmol TE/g). The high-fat diet promoted weight gain starting from the eighth week of the experiment. While jaboticaba delayed and reduced body weight gain, acai highly exacerbated it, also increasing the accumulation of fats. Colitis was successfully induced as seen by the clinical signs and damaged colonic mucosa. The high-fat diets did not increase the severity of colitis in comparison with the standard regimen, despite promoting additional weight gain. Animals that received acai or jaboticaba did not have many symptoms of colitis and showed a grand recovery in colonic histological parameters. Additionally, acai increased the expression of tight-junction-related molecules. Despite being included in a high-fat diet, the fruits still managed to promote healing effects in colitis, therefore indicating their potential for trials with IBD patients.

Pomegranate peel is an agro-industrial waste rich in phenolic compounds that can be employed like natural antioxidants in the food and pharmaceutical industry. In this study, five choline chloride-based natural deep eutectic solvents (NADES), which contained glucose, sucrose, glycerol, lactic acid, and citric acid as a hydrogen bond donor, were physicochemically characterized and tested for recovery of phenolics from pomegranate peel. The efficiency of the NADES in extracting phenolics from pomegranate peel has also been compared with that of conventional ethanolic extraction. The choline chloride and lactic acid (CC-LAC) NADES proved to be the most appropriate for the extraction of phenolics from pomegranate peel (4.14 mg EAG mL−1) if compared to the other tested NADES (range 0.81–3.84 mg EAG mL−1) and also if compared with the ethanolic solution (3.55 mg EAG mL−1). The optimization of the extraction conditions of phenolics from pomegranate waste peels using CC-LAC was carried out by multivariate strategies such as factorial design and response surface methodology. The optimal extraction conditions were 20 g of CC-LAC per g of pomegranate peel, 35% of lactic acid in relation to choline chloride, and 25 min of extraction at 45 °C. Under optimal extraction conditions, the phenolic recovery increased by 54.6%, when compared to CC-LAC non-optimized, and by 84.2%, when compared to ethanolic extraction. Our results, therefore, demonstrated that the use of NADES is a simple, cost-effective, more sustainable, and greener alternative for the extraction of the phenolics from pomegranate peel, over conventional extraction methods.

The Maillard reaction is responsible for color and flavor formation in fried, roasted and baked foods. However, this reaction can also promote the formation of acrylamide, a potential human carcinogen. The application of l -asparaginase represents a promising method for acrylamide mitigation in heated products by the direct conversion of the precursor l -asparagine to l -aspartic acid and ammonia, which does not lead to additional acrylamide formation. Our research group produced and purified an l -asparaginase from native Aspergillus oryzae CCT 3940 with highly potential for acrylamide mitigation. To verify the enzymatic effectiveness, we compared the native l -asparaginase with the commercial recombinant enzyme. For this purpose, a GC-MS/MS method was developed, optimized and validated for the assessment of acrylamide in French fries. Our results indicate a relationship between the l -asparagine content and acrylamide formation in French fries. The acrylamide concentration of the fried potato treated with the l -asparaginase from A. oryzae CCT 3940 and treated with commercial enzyme reduced 72% and 92%, respectively compared to control sample. Moreover, the l -asparaginase from A. oryzae CCT 3940 showed no l -glutaminase activity, while commercial enzyme promoted a decrease in the l -glutamine content (25%). Our results suggest that l -asparaginase from A. oryzae CCT 3940 may be of great value for acrylamide mitigation.

Natural compounds could be a complementary alternative to inflammatory bowel disease (IBD) management. This study determined the effects of an aqueous extract of Myrciaria jaboticaba peel (EJP) (50 g L−1) on 2,4,6-trinitrobenzenesulfonic acid-induced colitis. Wistar rats were randomized into five groups: HC—healthy control, CC—colitis control, DC—drug control, SJ—short-term treatment with EJP, and LJ—long-term treatment with EJP. The EJP treatments reduced body weight loss, stool consistency score, and spleen enlargement. Gut microbiota was modulated through increased Lactobacillus and Bifidobacterium counts after EJP treatment. Short-chain fatty acids were also higher in the EJP treatment groups. The antioxidant enzyme activities were greater than CC or DC controls. Myeloperoxidase activity (LJ), inducible nitric oxide synthase (LJ/SJ), and intercellular adhesion molecule (SJ) levels were lower than in the CC group. EJP decreased histological scoring, mucosal thickness, and preserved the crypts and histological structure. Therefore, EJP showed beneficial effects and could be potentially used as an adjuvant in IBD treatment.

Natural compounds could be a complementary alternative to inflammatory bowel disease (IBD) management. This study determined the effects of an aqueous extract of Myrciaria jaboticaba peel (EJP) (50 g L&minus, 1) on 2,4,6-trinitrobenzenesulfonic acid-induced colitis. Wistar rats were randomized into five groups: HC&mdash, healthy control, CC&mdash, colitis control, DC&mdash, drug control, SJ&mdash, short-term treatment with EJP, and LJ&mdash, long-term treatment with EJP. The EJP treatments reduced body weight loss, stool consistency score, and spleen enlargement. Gut microbiota was modulated through increased Lactobacillus and Bifidobacterium counts after EJP treatment. Short-chain fatty acids were also higher in the EJP treatment groups. The antioxidant enzyme activities were greater than CC or DC controls. Myeloperoxidase activity (LJ), inducible nitric oxide synthase (LJ/SJ), and intercellular adhesion molecule (SJ) levels were lower than in the CC group. EJP decreased histological scoring, mucosal thickness, and preserved the crypts and histological structure. Therefore, EJP showed beneficial effects and could be potentially used as an adjuvant in IBD treatment.

A suitable method based on headspace solid-phase microextraction (HS-SPME), comprehensive two-dimensional gas chromatography, with a time-of-flight mass spectrometry detector (GC × GC-TOFMS) and a chemometric approach was used aimed at the investigation of the volatile fraction of Brazilian Capsicum peppers: malagueta (C. frutescens), dedo-de-moca (C. baccatum) and murupi (C. chinense). A total of 184 volatiles compounds were identified in the three pepper samples and 123 of these compounds were described in Brazilian peppers for the first time. In addition, during Brazilian chili pepper maturation, as the maturation time increases, it was noted that the majority of volatile compounds responsible for green odor notes disappeared being replaced by others with fruity notes. Chemometric analysis (PCA) was able to separate samples according to their constituents, where malagueta was characterized by branched esters, murupi by terpenes and dedo-de-moca by the presence of aldehydes and terpenes.

Passion fruit (Passiflora edulis) peel, a waste from food industrial processing, is still little explored. It contains fibers, including soluble fibers, which may be a substrate for enteric bacteria and contribute to maintaining bowel health. The purpose of this work was to investigate the action of P. edulis peel in the microbiota, production of short-chain fatty acids (SCFAs), and antioxidant potential in the colon of Wistar rats. Seventy-seven-day-old male Wistar rats were divided into two groups: Peel and Control (n = 4). Both groups were fed with standard diet (AIN-93M); however, 50% of the cellulose content in the Peel group diet was replaced by fiber from P. edulis peel flour (PPF). After 15 days, the animals were anesthetized and sacrificed. The results showed that PPF intake positively affected the SCFAs intestinal production. The Peel group had greater butyrate and acetate concentration in cecal content than the Control group (P < 0.05), without alteration in microbiota (counts of Lactobacillus, Bifidobacterium, Enterobacteriaceae, and Total aerobic). Butyrate is a main substrate of the colonocytes and may improve mucus production, vascular flow, and mucosa barrier function. Changes in the antioxidant enzymes activity (GR, GPx, and SOD) and thiol groups (GSH) were not observed in the Peel group, which suggests that the period of PPF intake was insufficient to affect the oxidative status in the colon. These findings suggest that the Passiflora peel flour may improve bowel health by increasing SCFAs production, although more investigations are necessary about the effect of PPF on the colonic fermentation and the antioxidant status of the colon.

Inflammatory bowel disease is a chronic relapsing disease that affects millions of people worldwide; its pathogenesis is influenced by genetic, environmental, microbiological, and immunological factors. The aim of this study was to evaluate the effects of short- and long-term Passiflora edulis peel intake on the antioxidant status, microbiota, and short-chain fatty acids formation in rats with 2,4,6-trinitrobenzenesulphonic acid-induced colitis using two “ in vivo” experiments: chronic (prevention) and acute (treatment). The colitis damage score was determined using macroscopic and microscopic analyses. In addition, the antioxidant activity in serum and other tissues (liver and colon) was evaluated. Bifidobacteria, lactobacilli, aerobic bacteria and enterobacteria, and the amount of short-chain fatty acids (acetic, butyric, and propionic acids) in cecum content were counted. Differences in the colon damage scores were observed; P. edulis peel intake improved serum antioxidant status. In the treatment protocol, decreased colon lipid peroxidation, a decreased number of aerobic bacteria and enterobacteria, and an improvement in acetic and butyric acid levels in the feces were observed. An improvement in the bifidobacteria and lactobacilli was observed in the prevention protocol. These results suggested that P. edulis peel can modulate microbiota and could be used as source of fiber and polyphenols in the prevention of oxidative stress through the improvement of serum and tissue antioxidant status.

The biotechnological production of aromatic compounds is an important alternative to the chemical synthesis of flavor ingredients in food industry. Therefore, the development of an accurate and precise analytical technique for the measurement of the biotechnological production of aromatic compounds is of significant importance. In this paper, we optimize the extraction conditions for headspace solid-phase microextraction coupled to GC-FID and GC-MS for the determination of ethyl hexanoate produced by Neurospora sitophila in a malt-extract medium. Following three SPME fibers were tested: polydimethylsiloxane (PDMS), carboxen-polydimethylsiloxane (CAR/PDMS) and polydimethylsiloxane-divinylbenzene (PDMS/DVB). The PDMS/DVB was the most efficient fiber for trapping the volatile aromatic compound. The optimized SPME extraction temperature and time were 30 °C and 10 min, respectively. This method showed good linearity and the limits of detection (LOD) and limits of quantification (LOQ) of the targeted aromatic compound were 0.6 and 1.9 mg L−1, respectively. The HS-SPME methodology proved to be a simple and rapid approach to quantify ethyl hexanoate accurately and precisely for the biotechnological production process.

Passion fruit (Passiflora edulis), including its leaves, is a rich source of bioactive compounds, as polyphenols. This study investigated the in vitro and in vivo antioxidant potential of aqueous extract of P. edulis leaves and identification of phenolic compounds by HPLC-PDA and ESI-MS/MS analysis. Male Wistar rats were divided in two groups (n = 6 per group). Control group received water and experimental group received P. edulis leaf extract (Tea) (1.1 mg dry leaves mL− 1), both ad libitum. Total phenols and antioxidant potential (DPPH, FRAP, ABTS and ORAC assay) were determined in aqueous extract. Antioxidant status was analyzed by FRAP, ORAC in serum and by SOD, GR and GPx activities, GSH contents and TBARS in liver, brain and kidneys. Gut microbiota profile and short-chain fatty acids were determined in feces. Vitexin, isovitexin and isoorientin were analyzed in the extract of P. edulis leaves. The animals which received tea showed a decrease of 20% of TBARS in liver. GSH contents in kidneys increased 40% relative to control group. The GR activity was 2 times higher and GPx 3.2 times lower in liver than control group. Animals from TEA group showed a 45% reduction on SOD activities in liver and brain. Serum antioxidant potential was not altered. Tea intake also promoted colonic bacteria growth, although there was a decreasing in the SCFA production. Therefore, P. edulis leaf extract could be an option to enhance the supply of antioxidants and to safeguard against oxidative stress.

http://dx.doi.org/10.5902/198050989285Os metabólitos secundários extraídos de essências florestais conhecidamente micossimbiontes podem estimular o crescimento de isolados ectomicorrízicos in vitro. Assim, determinou-se o efeito de concentrações do óleo essencial de Eucalyptus grandis no estímulo ao crescimento dos fungos ectomicorrízicos Pisolithus sp. (UFSC Pt 24 e UFSC Pt 188), Pisolithus microcarpus (UFSC Pt 116), Chondrogaster angustiporus (UFSC Ch 163), Scleroderma citrinum (UFSC Sc 124) e Suillus sp. (UFSM RA 2.2 e UFSM RA 2.8) em meio de cultura líquido. Após o período de 25 dias de incubação, avaliou-se a morfologia e o crescimento dos isolados. A adição do óleo essencial nas concentrações de 20 a 30 μL L-1 promoveu crescimento miceliano in vitro nos isolados UFSC Pt 24, UFSC Pt 116, UFSC Ch 163, UFSC Sc 124, UFSM RA 2.2 e UFSM RA 2.8. A adição do óleo essencial na concentração de 20 μL L-1 resultou em aumento no diâmetro e na ramificação das hifas dos isolados UFSC Pt 116 e UFSC Pt 24. A utilização de óleo essencial na elaboração de meios de cultura para o crescimento de isolados ectomicorrízicos mostra-se eficiente, por aumentar a produção de massa micelial do fungo.

The aim of this study was to investigate the relationship between fructooligosaccharide (FOS) intake at different life stages of Wistar rats and its stimulatory effects on intestinal parameters. Recently weaned and ageing female rats were divided into growing and ageing treatments, which were fed diets that partially replaced sucrose with FOS for 12 weeks. Dietary FOS intake induced a significant increase in the numbers of Bifidobacterium and Lactobacillus in growing rats. FOS intake was associated with increased butyric acid levels and a reduced pH of the caecal contents at both ages. Differential gene expression patterns were observed by microarray analysis of growing and ageing animals fed the FOS diet. A total of 133 genes showed detectable changes in expression in the growing rats, while there were only 19 gene expression changes in ageing rats fed with FOS. These results suggest that dietary FOS intake may be beneficial for some parameters of intestinal health in growing rats.

Mate herb, Ilex paraguariensis St. Hil., is a plant cultivated in South America. Studies have evidenced the presence of phenolic compounds in mate herb, however, there are no reports on the contents of these compounds in different types of mate herb sold in the South Region of Brazil. In this study, capillary zone electrophoresis was used to quantify phenolic compounds in three lots of each of four types of commercial mate herb (traditional, native, large ground, and suave). Analysis was performed before and after acid hydrolysis. Hydrolysis time, temperature and chloridric acid concentration were optimized by central composite design to maximize the amounts of free phenolic compounds obtained after hydrolysis. Before hydrolysis, high amounts of rutin were detected, while after hydrolysis, high amounts of caffeic acid and 3,4-dihydroxybenzoic acid were obtained, giving evidence that mate herb should be further investigated as a major source of these compounds.

The volatile fractions of three varieties of chilli peppers at two stages of maturity were characterised using headspace solid phase micro-extraction (HS-SPME), gas chromatography (GC-FID) and mass spectrometry (GC–MS). A total of 83 compounds, mostly esters and alcohols, were identified in the malagueta chilli pepper ( Capsicum frutescens ), 49 compounds, mostly monoterpenes and aldehydes, were identified in the dedo-de-moca chilli pepper ( Capsicum baccatum var. pendulum ) and 77 compounds, mostly esters and sesquiterpenes, were identified in the murupi chilli pepper ( Capsicum chinense ). The peak areas of the compounds were smaller in the more mature samples. The samples from C. baccatum var. pendulum exhibited the greatest decrease; most of the compounds were not detected in the more mature samples.

Previous studies have assessed the properties of aqueous extracts, using byproducts such as jaboticaba peel. We have assessed potential antioxidant effects of jaboticaba extract (Plinia jaboticaba) (JAE = 50 g/L) in vitro and in vivo. Healthy Wistar rats received ad libitum JAE for either 15 or 49 days in vivo. Cyanidin-3-O-glucoside, delphinidin-3-O-glucoside, gallic acid, rutin, myricetin, and quercetin were identified as the main polyphenols in JAE. Lipid peroxidation values in the serum and colon were similar throughout the groups. In addition, JAE did not disturb the antioxidant systems. JAE also altered gut microbiota, increasing since Lactobacillus, Bifidobacterium and Enterobacteriaceae counts. Bacterial metabolites were higher in the colon content of rats fed with JAE than in the control group. Given these results, under healthy conditions, JAE dietary supplementation could perform in vivo modulation of gut microbiota, without disturbing the antioxidant system. PRACTICAL APPLICATION: Jaboticaba (Plinia jaboticaba) peel is a rich and often-wasted source of bioactive compounds, such as polyphenols. Previous studies have shown that physiological benefits of this berry. The jaboticaba peel could contribute to antioxidant defense systems; it may also have an effect over gut microbiota related to polyphenols contents. Aqueous extraction may be a practical way of employing the bioactive compounds of jaboticaba peel; these compounds can be consumed daily and safely, and thus have attracted particular attention. This work showed positive impacts of jaboticaba peel treatments on microbiota and antioxidant defense systems, and could guide future clinical studies.

The antioxidant and anti-inflammatory effects of the aqueous extract of Passiflora edulis leaves, which is a source of vitexin, isovitexin and isoorientin, was investigated in a 2,4,6-trinitrobenzenesulphonic acid induced colitis model. The oral intake of P. edulis extract (1100 µg mL−1) improved significantly (P

Com o objetivo de conhecer a qualidade, com destaque para os níveis de cobre, foram analisadas 25 amostras de cachaça artesanal, produzidas e comercializadas em 17 municípios pertencentes às microrregiões Ijuí, Cruz Alta, Santa Rosa e Três Passos, que integram o noroeste do Rio Grande do Sul, Brasil e, compararam-se os valores encontrados, com aqueles estabelecidos pela Legislação. Para as análises, procedeu-se a avaliação dos aspectos gerais das amostras através de observações visuais e olfativas, conforme as recomendações do Ministério da Agricultura para análise de bebidas alcoólicas destiladas. O teor alcoólico foi determinado utilizando-se método densimétrico e os teores de cobre foram determinados através de espectrofotometria de absorção atômica (EAA). Aplicou-se os testes de ANOVA e Tukey. Foram constatados, a partir da análise dos aspectos gerais, que 72% das amostras apresentaram anormalidades de limpidez. Verificou-se que a Microrregião Três Passos apresentou 42,86% de suas amostras fora dos limites estabelecidos pela legislação para o teor alcoólico, entretanto os valores médios deste mesmo parâmetro, para todas as amostras das quatro microrregiões, situaram-se dentro do que estabelece a legislação. Quanto aos níveis de cobre, a maior concentração média foi observada na Microrregião Três Passos (10,22mg L-1) enquanto que na Microrregião Cruz Alta foram observados os menores níveis médios deste metal (2,95mg L-1). Apenas as microrregiões Ijuí e Três Passos apresentaram diferenças estatisticamente significativas (P

Com o objetivo de monitorar os resíduos de compostos tóxicos em alimentos, verificou-se a freqüência e os níveis de praguicidas organoclorados (OC) e bifenilas policloradas (PCBs) em amostras de salsichas Hot-Dog, comercializadas na Cidade de Santa Maria, Estado do Rio Grande do Sul (RS) e, compararam-se os valores com aqueles estabelecidos pela Legislação. Para as análises, procedeu-se a extração da gordura das amostras. A partir da purificação desta, obteve-se um extrato do qual se determinaram os resíduos organoclorados por cromatografia gasosa com detector de captura eletrônica (CG - µECD63Ni). Verificou-se que o lindano e o HCB foram os pesticidas que apresentaram as maiores concentrações médias (0,001798ppm e 0,001652ppm, respectivamente). O somatório dos níveis médios dos pesticidas organoclorados detectados foi de 0,015783ppm, sendo que deste total 0,011165ppm pertencia aos metabólitos do DDT. Quanto as bifenilas policloradas, as maiores concentrações médias corresponderam às PCBs 10 (0,001817ppm) e 28 (0,000317ppm). O somatório das PCBs foi de 0,002172ppm. As concentrações de praguicidas organoclorados e PCBs encontradas nas amostras analisadas estavam abaixo dos limites máximos permitidos pela legislação.

A method involving headspace-solid phase micro-extraction (HS-SPME), gas chromatography with flame ionization detection (GC-FID) and gas chromatography with mass spectrometry (GC-MS) was developed and optimized to investigate the volatile composition of Capsicum chili peppers. Five SPME fibers were tested for extraction: carboxen/polydimethylsiloxane (CAR/PDMS-75μm), polydimethylsiloxane (PDMS-100μm), divinylbenzene/polydimethylsiloxane (DVB/PDMS-65μm), carbowax/divinylbenzene (CW/DVB-70μm), and divinylbenzene/carboxen/polydimethylsiloxane (DVB/CAR/PDMS-50/30μm), the last of which was shown to be the most efficient fiber to trap the volatile compounds. Optimization of the extraction conditions was carried out using multivariate strategies such as factorial design and response surface methodology. Eighty three compounds were identified by GC-MS when using the optimized extraction conditions, the majority of which were esters.

Foram investigadas as concentracoes dos agrotoxicos organoclorados lindano, HCB e p,p’- DDE em leite esterilizado e calculada a ingesta diaria desses compostos por criancas matriculadas na primeira serie do Ensino Fundamental, no municipio de Santa Maria, RS (BRASIL). As concentracoes de lindano (17,9 ng/g de gordura ± 26,5), HCB (3,4 ng/g de gordura ± 1,5) e p,p’-DDE (6,5 ng/g de gordura ± 4,4) nas amostras de leite enquadraram- se abaixo do limite maximo de residuos estabelecido pelo Codex Alimentarius (FAO, 1993). O consumo medio de leite esterilizado pelas criancas foi de 390,9 mL/dia. A ingesta diaria estimada de compostos organoclorados (0,210 µg/dia de lindano; 0,039 µg/dia de HCB e 0,076 µg/dia de p,p’-DDE) a partir de leite esterilizado ficou abaixo da ingesta diaria admissivel estabelecida pelo Codex Alimentarius. Conclui-se que o leite comercializado no municipio de Santa Maria nao representa risco toxicologico sob o aspecto avaliado.

Antibiotic growth promoter has been effectively used in poultry production for stimulating growth and preventing diseases. However, the use of antibiotics has been questioned due to the possible appearance of resistant bacteria. Special attention has been paid to the use of essential oils as natural alternatives for antibiotics. We investigated the use of different concentrations of microencapsulated eucalyptol and eugenol in broiler chickens diet as alternative growth promoters for avilamycin. One-day-old male broiler chickens were submitted to 7 different dietary treatments. The eugenol demonstrated strong antioxidant activity and both eugenol and eucalyptol showed antimicrobial activity. The broiler performance showed no significant difference to the group with administration of 10 mg/kg of avilamycin when eucalyptol (500 mg/kg) or eugenol (500 mg/kg) were used separately in diet. Our findings suggest that the microencapsulated phytotherapic agents are a potential alternative to the use of avilamycin as growth promoter in broilers during the broilers growing phase. DOI: 10.14685/rebrapa.v5i1.157

Os bifenilos policlorados (PCBs) e pesticidas organoclorados (OCPs) foram intensamente utilizados na agricultura e indústria até os anos 1970, quando efeitos tóxicos à saúde humana causados pela exposição aos PCBs e OCPs foram observados. Desde então, legislações internacionais levaram à regulamentação ou banimento do uso, e também o monitoramento de contaminação em alimentos. O consumo de leite começa na primeira infância como substituição à amamentação. A fração lipídica do leite pode reter compostos apolares como os PCBs e OCPs, o que torna a ingestão uma forma de exposição não-ocupacional a esses compostos. Esta tese teve o objetivo de desenvolver métodos analíticos modernos para o monitoramento de PCBs e OCPs em leite animal. No capítulo 1, há uma abrangente revisão sobre as características químicas dos PCBs e OCPs. A relevância das técnicas de preparo de amostra mais usuais para extração de PCBs e OCPs de leite também são abordadas e, no final do capítulo, é apresentada uma breve discussão sobre desenvolvimentos microfluídicos para detectar compostos organoclorados em amostras de leite. O capítulo 2 contém a seleção do método de preparo de amostra mais apropriado para a extração de PCBs de amostras de leite integral ultrapasteurizado (UHT). Uma microextração em fase sólida (SPME) e QuEChERS miniaturizada foram testadas e otimizadas usando planejamento experimental, seguido de análise por cromatografia gasosa acoplada à espectrometria de massas sequencial (GC- MS/MS). Apesar de a SPME ter apresentado melhor sensibilidade, optou-se por utilizar o método miniaturizado de QuEChERS para as análises subsequentes, vista a indisponibilidade de sistema automatizado para extração e injeção por SPME. A QuEChERS miniaturizada mostrou ser uma alternativa bastante viável para a extração de PCBs de leite UHT, oferecendo um reduzido custo por análise, sendo uma mais ambientalmente amigável que a QuEChERS em escala convencional. Seguindo, o capítulo 3 apresenta a validação analítica da QuEChERS miniaturizada para 9 PCBs (congêneres 28, 52, 101, 118, 138, 153, 156, 179 e 180) de acordo com as orientações do INMETRO e SANTE/11813/2017. O monitoramento da contaminação de PCBs em amostras de leite UHT do estado de São Paulo foi efetuado em 81 amostras de marcas locais; nenhuma delas apresentou níveis de concentração de PCBs acima dos limites de detecção. Por fim, no capítulo 4 é apresentado o desenvolvimento de um microdispositivo baseado na detecção condutométrica sem contato capacitivamente acoplada (C4D) para detecção específica de DDT através de sistema de bioreconhecimento utilizando a abordagem aptâmero-ligante. Uma microfabricação de baixo custo foi possível usando máscaras de papel adesivo, adesivo dupla-face e lamínulas de vidro como substrato. Os microdispositivos foram funcionalizados usando glutaraldeído e um aptâmero específico para DDT; a interação ligante-aptâmero promoveu uma redução significativa do sinal de C4D, mostrando que o aptasensor pode ser empregado como dispositivo semi-quantitativo para rápida e fácil avaliação da presença de DDT em amostras de alimentos ou ambientais. Polychlorinated biphenyls (PCBs) and organochlorine pesticides (OCPs) were extensively used for agricultural and industrial purposes until the 1970s when toxicity effects to human health caused by exposure PCBs and OCPs were noticed. Since then, international laws lead to regulate or banish their usage and also monitor food contamination. Animal milk consumption is introduced in early childhood as a replacement for human breastfeeding. Milk\'s lipidic fraction might retain nonpolar compounds as PCBs and OCPs, which turns milk intake a form of non-occupational exposure. This thesis aimed at developing modern analytical methods for PCBs and OCPs monitoring in animal milk. In chapter 1, there is a comprehensive review of the chemical properties of PCBs and OCPs. The importance of the mostly used extraction techniques for PCBs and OCPs from milk samples are also broached, and at this chapter\'s end, is presented a brief discussion about microfluidic developments to detect organochlorinated compounds in milk samples. Chapter 2 contains a selection of the most appropriate sample preparation for PCBs extraction from ultra-high temperature (UHT) whole-milk. Solid-phase microextraction (SPME) and a miniaturized QuEChERS were tested and optimized using experimental designs, followed by analysis by gas chromatography coupled to sequential mass spectrometry (GC-MS/MS). Despite SPME method presented higher sensitivity, it was chosen to use the miniaturized QuEChERS procedure to subsequent analysis once an automatized system to perform SPME extractions and injections was not available. The miniaturized QuEChERS procedure showed a suitable alternative to PCBs extractions from UHT milk, offering a reduced cost per analysis and being considered a greener methodology. Next, chapter 3 presents analytical validation of the miniaturized QuEChERS for 9 PCBs (congeners 28, 53, 101, 118, 138, 153, 156, 170, and 180) according to INMETRO and SANTE/11813/2017 guidelines. An assessment of PCBs contamination in UHT milk samples from São Paulo state was performed in 81 samples purchased from local brands with a positive outcome: none of them presented PCBs concentration above detection limits. Lastly, in chapter 4, the development of a capacitively coupled contactless conductivity (C4D) detection-based microdevice is presented, with specific DDT detection through a biorecognition system using the aptamer-ligand approach. Low-cost fabrication of microdevices was feasible by utilizing paper masks, double-sided adhesives, and glass coverslips substrates. They were functionalized using glutaraldehyde and an aptamer for specific DDT detection; the aptamer-ligand interaction promoted a remarkable C4D signal reducing, showing that the proposed aptasensor can be used as a semi- quantitative device for a fast and easy evaluation of DDT presence in food or environmental samples.

Made available in DSpace on 2020-12-10T17:34:53Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-04-01. Added 1 bitstream(s) on 2021-07-15T14:37:03Z : No. of bitstreams: 1 S0100-40422020000400435.pdf: 486292 bytes, checksum: 6885b4b0e454e9aa57754c474f755176 (MD5) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Aroma is one of the most important sensory attributes for acceptance by beef consumers. The first step in analysing the volatile compounds associated with this attribute is their extraction from the food matrix, solid-phase microextraction has been widely used for volatile compound determination in meat. This study aimed to test six different solid-phase microextraction (SPME) fibre coating materials for their volatile compounds extraction efficiency for roasted beef and to optimize the extraction conditions using response surface methodology. Gas chromatography coupled with a flame ionization detector (GC-FID) and mass spectrometry (GC-MS) were used. The choice of SPME fibre coating was based in the total area obtained by GC-FID analysis for the six fibre coatings. The optimum time and temperature for SPME extraction was 60 degrees C/65 minutes. The mixed-phase fibre coatings showed the best results for extracting volatile compounds in roasted beef as higher number of compounds were identified. The carboxen/polydimethylsiloxane (CAR/PDMS) fibre extracted the largest number of compounds under the optimum extraction condition. Aldehydes were the predominant class of compounds found in roasted beef, followed by alcohols and hydrocarbons. Univ Estadual Paulista, Fac Ciencias Farmaceut, BR-14800903 Araraquara, SP, Brazil Univ Sao Paulo, Inst Quim Sao Carlos, BR-13560970 Sao Carlos, SP, Brazil Embrapa Pecuaria Sudeste, Rodovia Washington Luiz,Km 234, BR-13560970 Sao Carlos, SP, Brazil Univ Estadual Paulista, Fac Ciencias Farmaceut, BR-14800903 Araraquara, SP, Brazil CNPq: 472344/2012-9 CNPq: 134462/2014-9 CAPES: 001

O objetivo desta pesquisa foi investigar a influência de diferentes porta-enxertos (Limão Cravo, Citrumelo Swingle, Trifoliata e Trifoliata Flying Dragon) na composição química dos óleos essenciais e dos voláteis do suco integral de tangerinas Fremont IAC 543. Os óleos essenciais da casca dos frutos foram obtidos por hidrodestilação, e variaram de 0,36 a 0,47% (v/m), sendo que a tangerina cultivada em porta-enxerto Trifoliata Flying Dragon foi a que apresentou menor rendimento de óleo essencial. A caracterização química dos óleos essenciais foi realizada por cromatografia gasosa acoplada a espectrometria de massas (GC-MS) e a semi-quantificação dos componentes dos óleos essenciais foi realizada por cromatografia gasosa acoplada a detector de ionização em chama (GC-FID). Os compostos majoritários dos óleos essenciais das variedades estudadas foram limoneno, linalol e mirceno, os quais são responsáveis por mais de 95% da área relativa dos componentes dos óleos essenciais. Devido à maior produtividade do porta-enxerto Citrumelo Swingle (18 L/ha), este é o mais indicado para produção de óleo essencial de tangerina Fremont IAC 543. Para a caracterização dos voláteis presentes no suco integral da tangerina Fremont IAC 543 foi realizada a otimização multivaridada da extração dos voláteis do suco por microextração em fase sólida de headspace (HS-SPME). Encontrou-se que a melhor condição para a extração dos voláteis consistiu no uso de fibra de divinilbenzeno/polidimetilsiloxano (DVB/PDMS), temperatura de extração de 43 °C e tempo de 31 min. Os principais compostos voláteis dos sucos integrais estudados foram o limoneno, mirceno, linalol, decanal, α-pineno, havendo bastante variação na composição dos voláteis dos sucos integrais de acordo com o porta-enxerto utilizado. Os espectros de Ressonância Magnética Nuclear mostraram que os sucos das quatro amostras analisadas possuem perfis complexos, porém semelhantes. A tangerina cultivada com porta-enxerto de Limão Cravo apresentou maiores valores de ácido málico e tirosina. A tangerina enxertada com Citrumelo Swingle apresentou maiores quantidades de ácido cítrico e etanol. A variedade plantada sobre o porta-enxerto de Trifoliata apresentou maiores valores de alanina, glicose e sacarose. A amostra cultivada sobre o porta-enxerto de Trifoliara Flying Dragon apresentou menor quantidade de etanol. Através da análise de componentes principais foi possível diferenciar as amostras a partir de seus componentes de óleo essencial e compostos voláteis e não-voláteis do suco integral de cada variedade plantada em diferentes porta-enxertos. The objective of this research was to investigate the influence of different rootstocks (Clove Lemon, Citrumelo Swingle, Trifoliata and Trifoliata Flying Dragon) on the chemical composition of the essential oils and volatiles of the Fremont IAC 543 mandarin. The essential oils from the fruit peels were obtained by hydrodistillation, and ranged from 0.36 to 0.47% (v/w) and the mandarin cultivated in Trifoliata Flying Dragon rootstock showed the lowest essential oil yield. The chemical characterization of the essential oils was performed by gas chromatography coupled to mass spectrometry (GC-MS) and the semi-quantification of essential oil components was performed by gas chromatography coupled to a flame ionization detector (GC-FID). The major essential oils compounds were limonene, linalool, and myrcene, which were responsible for more than 95% of the essential oil components. Due to the higher productivity of the Citrumelo Swingle rootstock (18 L/ha), this was the most suitable rootstock for the Fremont IAC 543 mandarin essential oil production. Aiming the characterization of the Fremont IAC 543 mandarin juice volatiles compounds, a multivariate optimization of juice volatiles extraction by headspace solid phase microextraction (HS-SPME) was performed. It was found that the best volatile extraction conditions were the use of divinylbenzene/polydimethylsiloxane (DVB/PDMS) fiber, 43 ° C for extraction temperature and 31 min for extraction time. The main volatile compounds of the whole juices studied were limonene, myrcene, linalool, decanal and α-pinene. It possible see a considerable variation in the volatile composition of whole juices according to the rootstock used. Nuclear Magnetic Resonance spectras showed that the juices of the four samples analyzed had complex but similar profiles. Tangerine cultivated with Lemon Cravo rootstock showed higher values of malic acid and tyrosine. Tangerine growth in Citrumelo Swingle showed higher amounts of citric acid and ethanol. The variety planted on the rootstock of Trifoliata showed higher values of alanine, glucose and sucrose. The sample cultivated on the Trifoliara Flying Dragon rootstock showed less ethanol. Through the principal component analysis, it was possible to differentiate the samples using their essential oil components and volatile and non-volatile compounds from the integral juice of each variety planted in different rootstocks.

O objetivo desta pesquisa foi identificar os voláteis importantes para o aroma da carne de frangos suplementados com diferentes concentrações de beta-ácidos do lúpulo. Para isso, 1440 frangos de corte Cobb 500 foram alimentados ad libitum durante 42 dias com dietas basais suplementadas com 0 (controle negativo), 30, 60 e 120 mg kg-1 de β- ácidos do lúpulo. Após 42 dias de experimento, os animais foram abatidos e o músculo peitoral maior (peito do frango) foi retirado, separado do tecido conjuntivo, da pele e da gordura subcutânea. Os filés de peito foram imediatamente congelados a -80 °C até o momento das análises. Nos filés foi determinado o perfil de ácidos graxos por cromatografia gasosa acoplada a detector de ionização em chama. E a carne dos animais suplementados com 30 mg kg-1 de β-ácidos foi a que apresentou a maior quantidade dos ácidos graxos insaturados de cadeia longa araquidônico (C20:4-n6) e docosaexaenoico (C22:6-n3), cujas propriedades antioxidantes e anti-inflamatórias são benéficas para saúde humana. Para a caracterização dos voláteis da carne assada foi desenvolvido e otimizado um procedimento de extração dos voláteis da carne por microextração em fase sólida de headspace (HS-SPME) e cromatografia gasosa acoplada à espectrometria de massa (GC-MS). Para isso, foram testados cinco tipos de fibras comerciais de SPME quanto a sua capacidade de extração dos voláteis da carne assada de frango e, para fibra que apresentou maior capacidade de extração (CAR/PDMS), foram otimizadas as condições de temperatura e tempo de extração por meio de delineamento composto central rotacional. As condições ótimas de extração foram: temperatura de extração de 81°C e tempo de extração de 45 min. Foram identificados 70 compostos voláteis na carne assada de frango por GC-MS majoritariamente aldeídos, alcanos, álcoois, ésteres e pirazinas. A análise de componentes principais (PCA) foi capaz de separar as amostras em função da suplementação com beta ácidos, o que indica que os diferentes níveis de suplementação podem originar diferentes voláteis na carne assada. O tratamento controle negativo (0 mg kg-1 de β-ácidos) foi caracterizado principalmente por aldeídos e ácidos como octanal, decanal, benzaldeido e ácido n-hexanóico, o tratamento com 30 mg kg-1 de β-ácidos foi caracterizado por pirazinas (2, 6 - dimetil prirazina e 3- etil, 2, 5 dimetil pirazina), o tratamento com 60 mg kg-1 de β-ácidos foi caracterizado por uma maior quantidade de álcoois, aldeídos e furanos (hexanal, 1-octen-3 ol e pentilfurano), enquanto que o tratamento com 120 mg kg-1 de β-ácidos foi caracterizado pela presença docomposto 2- metil pentanal. The aim of this research was to identify volatiles that are important for the aroma of chicken meat supplemented with different concentrations of hops β-acids. To carry out this research, 1440 Cobb 500 broilers were fed ad libitum during 42 days with basal diets supplemented with 0 (negative control), 30, 60, 120 and 240 mg kg-1 of hops β-acids. After 42 days, the animals were slaughtered and the pectoralis major muscle (chicken breast) was removed, separated from the conjunctive tissue, the skin, and the subcutaneous fat. The breast fillets were immediately frozen at -80° C until the time of analysis. The fatty acid profile in the steaks was determined by gas chromatography with flame ionization detector (GC-FID). The meat of the animals supplemented with 30 mg kg-1 of β-acids presented the highest amount of long-chain unsaturated fatty acids such as arachidonic acid (C20:4-n6) and docosaexaenoico (C22:6-n3), which have antioxidant and anti-inflammatory properties benefical to human health. For the characterization of the roasted meat volatiles, an extraction procedure was developed and optimized by headspace solid-phase microextraction (HS-SPME) and gas chromatography coupled to mass spectrometry (GC-MS). Six different types of SPME commercial fibers were tested regarding their ability to extract volatiles from chicken roasted meat, and to the fiber that showed the highest extraction capacity (DVB/CAR/PDMS), temperature and time of extraction were optimized through central composite design. The optimum extraction conditions were: extraction temperature of 81 °C and extraction time 45 minutes. It was possible to characterize 70 volatiles in the volatile fraction of roasted chicken meat by GC-MS, The majority of them were aldehydes, alkanes, alcohols, esters and pyrazines. The Principal Component Analysis (PCA) was able to separate the samples according to the supplementation with hops β-acids, which indicates that different levels of supplementation may yield different volatile compounds on roasted chicken meat. The negative control treatment (0 mg kg-1 of β-acids) was characterized mainly by aldehydes and acids like octanal, decanal, benzaldehyde and n-hexanoic acid, the treatment with 30 mg kg-1 of β-acids was characterized by pyrazines (2,6 - dimethyl pyrazine, and 3-ethyl, 2,5- dimethyl pyrazine, the treatment with 60 mg kg-1 of β-acids was characterized by a major quantity of alcohols, aldehydes and furans (hexanal, 1-octen-3-ol and pentylfuran), whereas the treatment with 120 mg kg-1 of β-acids was characterized by the presence of 2 the compound 2-methyl pentanal.