1. The Truman Show for protozoan parasites: A review of in vitro cultivation platforms
- Author
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Martin H. Richter and Smita Sutrave
- Subjects
Plasmodium ,Life Cycles ,RC955-962 ,Review ,Medical Conditions ,Arctic medicine. Tropical medicine ,Medicine and Health Sciences ,Organ Cultures ,Protozoans ,biology ,Transmission (medicine) ,Eukaryota ,Organoids ,Infectious Diseases ,Neglected tropical diseases ,Biological Cultures ,Public aspects of medicine ,RA1-1270 ,Anatomy ,Trypanosoma ,Parasitic Life Cycles ,Zoology ,Research and Analysis Methods ,Host-Parasite Interactions ,Protozoan infection ,parasitic diseases ,Parasite Groups ,medicine ,Parasitic Diseases ,Animals ,Humans ,Parasitic life cycles ,Life Cycle Stages ,Protozoan Infections ,Public Health, Environmental and Occupational Health ,Organisms ,Oocysts ,Outbreak ,Biology and Life Sciences ,biology.organism_classification ,medicine.disease ,Parasitic Protozoans ,Gastrointestinal Tract ,Protozoa ,Parasitology ,Parasitic Intestinal Diseases ,Apicomplexa ,Digestive System ,Developmental Biology - Abstract
Protozoan parasites are responsible for severe disease and suffering in humans worldwide. Apart from disease transmission via insect vectors and contaminated soil, food, or water, transmission may occur congenitally or by way of blood transfusion and organ transplantation. Several recent outbreaks associated with fresh produce and potable water emphasize the need for vigilance and monitoring of protozoan parasites that cause severe disease in humans globally. Apart from the tropical parasite Plasmodium spp., other protozoa causing debilitating and fatal diseases such as Trypanosoma spp. and Naegleria fowleri need to be studied in more detail. Climate change and socioeconomic issues such as migration continue to be major drivers for the spread of these neglected tropical diseases beyond endemic zones. Due to the complex life cycles of protozoa involving multiple hosts, vectors, and stringent growth conditions, studying these parasites has been challenging. While in vivo models may provide insights into host–parasite interaction, the ethical aspects of laboratory animal use and the challenge of ready availability of parasite life stages underline the need for in vitro models as valid alternatives for culturing and maintaining protozoan parasites. To our knowledge, this review is the first of its kind to highlight available in vitro models for protozoa causing highly infectious diseases. In recent years, several research efforts using new technologies such as 3D organoid and spheroid systems for protozoan parasites have been introduced that provide valuable tools to advance complex culturing models and offer new opportunities toward the advancement of parasite in vitro studies. In vitro models aid scientists and healthcare providers in gaining insights into parasite infection biology, ultimately enabling the use of novel strategies for preventing and treating these diseases., Author summary In light of the far-reaching social and economic repercussions of communicable, zoonotic parasitic diseases on human health, it is imperative to continue to strive toward developing in vitro models for in-depth scrutiny and understanding of pathogenicity, as well as for innovations toward combating these infections. This review, to our knowledge, is the first to offer a qualitative summary of the existing models for culturing protozoan parasites with major relevance to human health in vitro. The present work aims to provide a reference guide on the current state of in vitro culture of these protozoan parasites and offers a foundation to facilitate exchange of expertise among researchers, clinicians, and healthcare workers. This comprehensive review would aid in enabling discussions on new intervention approaches to fill in the knowledge gaps in the field of parasitic diseases affecting the global population.
- Published
- 2021