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3 results on '"MESH: Thylakoids"'

1. Developmentally regulated association of plastid division protein FtsZ1 with thylakoid membranes in Arabidopsis thaliana

Author

El-Sayed El-Kafafi, Silva Lerbs-Mache, Isabelle Pignot-Paintrand, Mohamed Karamoko, Paul Mandaron, Denis Falconet, Didier Grunwald, Plastes et différenciation cellulaire (PDC), Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS), Centre de Recherches sur les Macromolécules Végétales (CERMAV), Université Joseph Fourier - Grenoble 1 (UJF)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), ANTE-INSERM U836, équipe 4, Muscles et pathologies, Transduction du signal : signalisation calcium, phosphorylation et inflammation, Université Joseph Fourier - Grenoble 1 (UJF)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Joseph Fourier - Grenoble 1 (UJF)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM), This work was supported by a grant [ACIDRAB (ActionConcertée Incitative-Dynamique et Réactivité des Assemblages Biologiques) 03/41, number 03 5 90 to D. F. and number 03 5 92 to I.P.-P.] from the CNRS and the MEN (Ministère de l'Education Nationale), by the Egyptian government (fellowship toE.-S. E.-K.), and by the Ivory Coast government (fellowship to M.K.)., Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF), Plastes et Différenciation Cellulaire, Université Joseph Fourier and CNRS, Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF), Alexandre, France, and Baudier, Jacques

Subjects
Chlorophyll, 0106 biological sciences, Chloroplasts, Plastid localization, Arabidopsis thaliana, Mutant, Arabidopsis, Thylakoids, 01 natural sciences, Biochemistry, MESH: Thylakoids, Gene Expression Regulation, Plant, chloroplast division, MESH: Microscopy, Confocal, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, MESH: Arabidopsis, Plastids, ComputingMilieux_MISCELLANEOUS, Plant Proteins, 2. Zero hunger, 0303 health sciences, Microscopy, Confocal, MESH: Plant Proteins, biology, Stem Cells, Life Sciences, food and beverages, thylakoid membrane, MESH: Plastids, Immunohistochemistry, Cell biology, Chloroplast, Phenotype, Thylakoid, MESH: Microscopy, Electron, Transmission, MESH: Chlorophyll, FtsZ gene family, MESH: Arabidopsis Proteins, MESH: Stem Cells, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, macromolecular substances, MESH: Phenotype, Models, Biological, 03 medical and health sciences, Microscopy, Electron, Transmission, plastid localization, [SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Gene family, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, MESH: Gene Expression Regulation, Plant, Plastid, FtsZ, [SDV.BC] Life Sciences [q-bio]/Cellular Biology, Molecular Biology, 030304 developmental biology, Organelles, MESH: Chloroplasts, Arabidopsis Proteins, MESH: Models, Biological, MESH: Immunohistochemistry, Cell Biology, biology.organism_classification, plastid division, biology.protein, MESH: Organelles, 010606 plant biology & botany
Abstract

International audience; FtsZ is a key protein involved in bacterial and organellar division. Bacteria have only one ftsZ gene, while chlorophytes (higher plants and green alga) have two distinct FtsZ gene families, named FtsZ1 and FtsZ2. This raises the question of why chloroplasts in these organisms need distinct FtsZ proteins to divide. In order to unravel new functions associated with FtsZ proteins, we have identified and characterized an Arabidopsis thaliana FtsZ1 loss-of-function mutant. ftsZ1-knockout mutants are impeded in chloroplast division, and division is restored when FtsZ1 is expressed at a low level. FtsZ1-overexpressing plants show a drastic inhibition of chloroplast division. Chloroplast morphology is altered in ftsZ1, with chloroplasts having abnormalities in the thylakoid membrane network. Overexpression of FtsZ1 also induced defects in thylakoid organization with an increased network of twisting thylakoids and larger grana. We show that FtsZ1, in addition to being present in the stroma, is tightly associated with the thylakoid fraction. This association is developmentally regulated since FtsZ1 is found in the thylakoid fraction of young developing plant leaves but not in mature and old plant leaves. Our results suggest that plastid division protein FtsZ1 may have a function during leaf development in thylakoid organization, thus highlighting new functions for green plastid FtsZ.

Published
2007

2. Fibrillin influence on plastid ultrastructure and pigment content in tomato fruit

Author

Joël Gaffé, Paul D. Fraser, Andrew J. Simkin, Peter M. Bramley, Jean-Pierre Carde, Marcel Kuntz, Jean-Pierre Alcaraz, Laboratoire Adaptation et pathogénie des micro-organismes [Grenoble] (LAPM), Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF), Gestes Medico-chirurgicaux Assistés par Ordinateur (TIMC-IMAG-GMCAO), Techniques de l'Ingénierie Médicale et de la Complexité - Informatique, Mathématiques et Applications, Grenoble - UMR 5525 (TIMC-IMAG), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF), Plastes et différenciation cellulaire (PDC), European commission Agriculture and Fisheries program for the mobility of researchers (Contract No. FAIR-98-5002), Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS), Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP)-IMAG-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP)-IMAG-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), Laboratoire de physiologie cellulaire végétale (LPCV), and Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Recherche Agronomique (INRA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)

Subjects
0106 biological sciences, Plastoglobule, macromolecular substances, Plant Science, MESH: Capsicum, Horticulture, Biology, Fibrillins, Thylakoids, Chloroplast, 01 natural sciences, Biochemistry, MESH: Microfilament Proteins, 03 medical and health sciences, MESH: Thylakoids, Solanum lycopersicum, Membrane pore, MESH: Lycopersicon esculentum, Chromoplast, Plastids, Lycopersicon esculentum, Thylakoid integrity, Plastid, Molecular Biology, Carotenoid, 030304 developmental biology, chemistry.chemical_classification, 0303 health sciences, [SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE], Microfilament Proteins, food and beverages, Ripening, MESH: Plastids, General Medicine, Plants, Genetically Modified, Fruit ripening, Carotenoids, MESH: Plants, Genetically Modified, chemistry, Fruit, Thylakoid, MESH: Carotenoids, Capsicum, MESH: Fruit, Fibrillin, 010606 plant biology & botany
Abstract

International audience; The protein termed fibrillin is involved in the formation of lipoprotein structures, such as plastoglobules and fibrils in certain chromoplast types, which have been implicated in the over-production of pigments due to a sink effect. In order to examine its effect in differentiating chromoplasts of a non-fibrillar type, the pepper fibrillin gene was expressed in tomato fruit. Both the transcript and protein were found to accumulate during tomato fruit ripening from an early mature green stage. However, formation of carotenoid deposition structures in tomato chromoplasts, such as fibrils, was not observed. Nevertheless, a two-fold increase in carotenoid content and associated carotenoid derived flavour volatiles (6-methyl-5-hepten-2-one, geranylacetone, beta-ionone and beta-cyclocitral) was observed. An unexpected phenotypic observation in the transgenic fruit was the delayed loss of thylakoids in differentiating chromoplasts, leading to the transient formation of plastids exhibiting a typical chromoplastic zone adjacent to a protected chloroplastic zone with preserved thylakoids. An in vitro assay has been developed to monitor fibrillin activity on thylakoids: data were obtained suggesting a membrane protection role for fibrillin, more specifically against moderate uncoupling effects.

Published
2007

3. Kinetics of structural reorganizations in multilamellar photosynthetic membranes monitored by small-angle neutron scattering

Author

Ottó Zsiros, Gergely Nagy, László Almásy, Peter Timmins, Győző Garab, László Kovács, Dorthe Posselt, Renáta Ünnep, Judith Peters, László Rosta, Institut Laue-Langevin (ILL), ILL, Dept Biol, University of Missouri [Columbia] (Mizzou), University of Missouri System-University of Missouri System, Wigner Research Centre for Physics [Budapest], Hungarian Academy of Sciences (MTA), Institute of Plant Biology, Biological Research Centre [Budapest] (BRC), Hungarian Academy of Sciences (MTA)-Hungarian Academy of Sciences (MTA), Department of Genetics, Southwest Foundation for Biomedical Research, Department of Data Management, Eo¨tvo¨s Lora'nd Geophysical Institute of Hungary, Geophysical Institute of Hungary, Institut de biologie structurale (IBS - UMR 5075 ), Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), and Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)

Subjects
0106 biological sciences, Materials science, Kinetics, Biophysics, Neutron scattering, Photosynthesis, 01 natural sciences, Thylakoids, 03 medical and health sciences, Nuclear magnetic resonance, MESH: Thylakoids, Spinacia oleracea, Scattering, Small Angle, General Materials Science, MESH: Scattering, Small Angle, 030304 developmental biology, 0303 health sciences, MESH: Kinetics, [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM], MESH: Spinacia oleracea, food and beverages, Time resolution, Surfaces and Interfaces, General Chemistry, Intracellular Membranes, Small-angle neutron scattering, MESH: Neutron Diffraction, MESH: Intracellular Membranes, Neutron Diffraction, Membrane, Thylakoid, lipids (amino acids, peptides, and proteins), 010606 plant biology & botany, Biotechnology
Abstract

International audience; We demonstrate the power of time-resolved small-angle neutron scattering experiments for the investigation of the structure and structural reorganizations of multilamellar photosynthetic membranes. In addition to briefly summarizing our results on thylakoid membranes isolated from higher plants and in unicellular organisms, we discuss the advantages and technical and methodological limitations of time-resolved SANS. We present a detailed and more systematical investigation of the kinetics of light-induced structural reorganizations in isolated spinach thylakoid membranes, which show how changes in the repeat distance and in the long-range order of the multilamellar membranes can be followed with a time resolution of seconds. We also present data from comparative measurements performed on thylakoid membranes isolated from tobacco.

Published
2012

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