Back to Search
Start Over
Fibrillin influence on plastid ultrastructure and pigment content in tomato fruit
- Source :
- Phytochemistry, Phytochemistry, Elsevier, 2007, 68 (11), pp.1545-56. ⟨10.1016/j.phytochem.2007.03.014⟩
- Publication Year :
- 2007
- Publisher :
- Elsevier BV, 2007.
-
Abstract
- International audience; The protein termed fibrillin is involved in the formation of lipoprotein structures, such as plastoglobules and fibrils in certain chromoplast types, which have been implicated in the over-production of pigments due to a sink effect. In order to examine its effect in differentiating chromoplasts of a non-fibrillar type, the pepper fibrillin gene was expressed in tomato fruit. Both the transcript and protein were found to accumulate during tomato fruit ripening from an early mature green stage. However, formation of carotenoid deposition structures in tomato chromoplasts, such as fibrils, was not observed. Nevertheless, a two-fold increase in carotenoid content and associated carotenoid derived flavour volatiles (6-methyl-5-hepten-2-one, geranylacetone, beta-ionone and beta-cyclocitral) was observed. An unexpected phenotypic observation in the transgenic fruit was the delayed loss of thylakoids in differentiating chromoplasts, leading to the transient formation of plastids exhibiting a typical chromoplastic zone adjacent to a protected chloroplastic zone with preserved thylakoids. An in vitro assay has been developed to monitor fibrillin activity on thylakoids: data were obtained suggesting a membrane protection role for fibrillin, more specifically against moderate uncoupling effects.
- Subjects :
- 0106 biological sciences
Plastoglobule
macromolecular substances
Plant Science
MESH: Capsicum
Horticulture
Biology
Fibrillins
Thylakoids
Chloroplast
01 natural sciences
Biochemistry
MESH: Microfilament Proteins
03 medical and health sciences
MESH: Thylakoids
Solanum lycopersicum
Membrane pore
MESH: Lycopersicon esculentum
Chromoplast
Plastids
Lycopersicon esculentum
Thylakoid integrity
Plastid
Molecular Biology
Carotenoid
030304 developmental biology
chemistry.chemical_classification
0303 health sciences
[SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE]
Microfilament Proteins
food and beverages
Ripening
MESH: Plastids
General Medicine
Plants, Genetically Modified
Fruit ripening
Carotenoids
MESH: Plants, Genetically Modified
chemistry
Fruit
Thylakoid
MESH: Carotenoids
Capsicum
MESH: Fruit
Fibrillin
010606 plant biology & botany
Subjects
Details
- ISSN :
- 00319422
- Volume :
- 68
- Database :
- OpenAIRE
- Journal :
- Phytochemistry
- Accession number :
- edsair.doi.dedup.....a4e76fc5ef66e90a229035d82b2387c5