Search

Additional file 3: Figure S2. a Sequence alignment among the deduced SBP-4 amino acid sequences from Egypt and USA B. caballi isolates (1, 2) and SBP4 from Babesia bovis (3). Consensus sequences are shown in the bottom row. bIn silico prediction of trans-membrane domains and signal peptides in Egyptian B. caballi SBP-4 using TMHMM2.

Additional file 5: Figure S4. a Anti-histidin antibodies recognize a 37 kDa antigen in a cell lysate of recombinant E. coli transformed with TOPO® TA plasmid containing the full-length Bcsbp4 gene. Lane 1: (+ ve) cultures induced with L-Arabinose; Lane 2: (- ve) non-Arabinose induced cultures. b Specific recognition of rBcSBP4 protein by B. caballi positive serum in ELISA (a 1:100 dilution of serum was serially diluted by two-fold) using: serum from an equid derived from a horse experimentally infected with B. caballi (blue columns); field horse positive for B. caballi infection (orange columns); and horse that tested negative to infection with B. caballi (grey columns).

Combined_supplemental_materials-Delaney_et_al for Avoidance of the NLRP3 Inflammasome by the Stealth Pathogen, Coxiella burnetii by Martha A. Delaney, Andreas den Hartigh, Samuel J. Carpentier, Timothy P. Birkland, Donald P. Knowles, Brad T. Cookson and Charles W. Frevert in Veterinary Pathology

Additional file 2: Figure S1. Features of the BcSBP4 protein: the BcSBP4 was predicted to be secreted. The location of the predicted signal peptide and the regions containing the two peptides selected for antibody production are indicated by a green rectangle. The predictions were performed using online software at http://wlab.ethz.ch/protter .

Additional file 6: Figure S5. Specificity of the anti BcSBP4 peptide rabbit antibodies: western blot analysis against (i) normal horse RBC lysate, (ii) B. caballi-infected RBC lysate, (iii) recombinant B. caballi Spb4 and (iv) recombinant B. bovis Hap2 using serum indicated above. Abbreviation: M, size markers.

Additional file 1: Dataset S1. Amino acid sequence of BcSPB4.

Additional file 4: Figure S3. Secondary structure prediction of B. caballi SBP4 ( http://minnou.cchmc.org/ ).