Additional file 5 of Identification and antigenicity of the Babesia caballi spherical body protein 4 (SBP4)

Additional file 5: Figure S4. a Anti-histidin antibodies recognize a 37 kDa antigen in a cell lysate of recombinant E. coli transformed with TOPO® TA plasmid containing the full-length Bcsbp4 gene. Lane 1: (+ ve) cultures induced with L-Arabinose; Lane 2: (- ve) non-Arabinose induced cultures. b Specific recognition of rBcSBP4 protein by B. caballi positive serum in ELISA (a 1:100 dilution of serum was serially diluted by two-fold) using: serum from an equid derived from a horse experimentally infected with B. caballi (blue columns); field horse positive for B. caballi infection (orange columns); and horse that tested negative to infection with B. caballi (grey columns).