1. Synergy between Lactobacillus paracasei and Its Bacterial Products to Counteract Stress-Induced Gut Permeability and Sensitivity Increase in Rats
- Author
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Lionel Bueno, Florence Rochat, Jean Fioramonti, Gabriela Bergonzelli, Helene Eutamene, Chantal Chabo, Vassilia Theodorou, Irene Corthesy-Theulaz, and Florence Lamine
- Subjects
Restraint, Physical ,Lactobacillus paracasei ,Pain ,Medicine (miscellaneous) ,Pharmacology ,Permeability ,law.invention ,Microbiology ,Probiotic ,Stress, Physiological ,law ,Lactobacillus ,medicine ,Animals ,Rats, Wistar ,Lactobacillus johnsonii ,Nutrition and Dietetics ,biology ,Visceral pain ,biology.organism_classification ,Rats ,Intestines ,Paracellular transport ,Hyperalgesia ,Bifidobacterium ,medicine.symptom ,Bacteria - Abstract
Stressful events result in the alteration of gut permeability and sensitivity. Lactobacillus paracasei NCC2461 (Lpa) therapy prevents antibiotic-induced visceral hyperalgesia in mice. This study aimed at evaluating the influence of 3 probiotic strains: Bifidobacterium lactis NCC362, Lactobacillus johnsonii NCC533, and Lpa on stress-mediated alterations of colorectal hyperalgesia, on gut paracellular permeability and whether bacteria and/or bacterial products present in the spent culture medium (SCM) were involved in the antinociceptive properties of the effective strain. Rat pups were separated from their mothers 3 h/d during postnatal d 2-14. At wk 13, gut paracellular permeability was determined as a percentage of urinary excreted (51)Cr-EDTA and visceral sensitivity to colorectal distension (CRD), assessed by abdominal muscle electromyography. Visceral sensitivity was also analyzed in adults rats subjected to partial restraint stress (PRS, 2 h restriction of body movements). Rats received either the probiotics resuspended in SCM or fresh growth medium as control for 2 wk. Maternal deprivation significantly increased colonic sensitivity in response to CRD and enhanced gut paracellular permeability compared with control rats. Only Lpa treatment significantly improved stress-induced visceral pain and restored normal gut permeability. Similarly, among the 3 probiotics tested, only Lpa prevented PRS-mediated visceral hyperalgesia. Both bacteria and bacterial products present in Lpa SCM were required for the antinociceptive properties against PRS. This study illustrates strain-specific effects and suggests a synergistic interplay between L. paracasei bacteria and bacterial products generated during fermentation and growth that confers the ability to suppress PRS-induced hypersensitivity in rats.
- Published
- 2007