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Immunization of BALB/c mice against Helicobacter felis infection with Helicobacter pylori urease
- Source :
- Gastroenterology. 107:1002-1011
- Publication Year :
- 1994
- Publisher :
- Elsevier BV, 1994.
-
Abstract
- Background/Aims: Because Helicobacter pylori is a potentially dangerous human pathogen, the protective potential of oral immunization with H. pylori urease and its subunits was evaluated in an animal model. Methods: Mice were orally immunized with H. pylori sonicate, urease, or recombinant enzymatically inactive urease subunits and then challenged with Helicobacter felis . Control mice were sham-immunized. Results: H. felis colonization was present 5 days after challenge in 9 of 10 sham-immunized, 6 of 9 sonicate-immunized, and 3 of 10 urease-immunized animals ( P = 0.031 vs. sham-immunized). Twelve days after challenge, urease B-immunized mice had a weaker colonization than sham-immunized controls, whereas urease A had no effect. After 70 days, most urease A- and urease B-immunized mice had cleared the colonization (1017: P=0.0019; 1620: P=0.00002 vs. sham-immunized). In urease B-immunized animals, protection was often associated with corpus gastritis. Conclusions: Oral immunization with H. pylori urease protects mice against H. felis infection. Enzymatically inactive urease A and B subunits contain protective epitopes. It is unclear whether protection depends on the development of a mononuclear inflammatory response in the gastric corpus. Our observations should encourage the development of a human vaccine.
- Subjects :
- Urease
animal diseases
Administration, Oral
Rapid urease test
chemical and pharmacologic phenomena
Cross Reactions
Helicobacter Infections
BALB/c
Microbiology
Mice
medicine
Animals
Mice, Inbred BALB C
Helicobacter pylori
Hepatology
biology
Stomach
Gastroenterology
Antibodies, Monoclonal
biochemical phenomena, metabolism, and nutrition
biology.organism_classification
Recombinant Proteins
Immunization
Gastritis
Helicobacter felis
biology.protein
bacteria
Female
Antibody
medicine.symptom
Subjects
Details
- ISSN :
- 00165085
- Volume :
- 107
- Database :
- OpenAIRE
- Journal :
- Gastroenterology
- Accession number :
- edsair.doi.dedup.....60fe1cde39911f8833ae339de8c81e51