1. Norbornene Probes for the Detection of Cysteine Sulfenic Acid in Cells
- Author
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Alcock, Lisa J, Oliveira, Bruno L, Deery, Michael J, Pukala, Tara L, Perkins, Michael V, Bernardes, Gonçalo JL, Chalker, Justin M, Alcock, Lisa J [0000-0001-9068-0756], Pukala, Tara L [0000-0001-7391-1436], Bernardes, Gonçalo JL [0000-0001-6594-8917], Chalker, Justin M [0000-0002-7504-5508], and Apollo - University of Cambridge Repository
- Subjects
Molecular Probes ,Cysteine ,Norbornanes ,Oxidation-Reduction ,Sulfenic Acids - Abstract
Norbornene derivatives were validated as probes for cysteine sulfenic acid on proteins and in live cells. Trapping sulfenic acids with norbornene probes is highly selective and revealed a different reactivity profile than the traditional dimedone reagent. The norbornene probe also revealed a superior chemoselectivity when compared to a commonly used dimedone probe. Together, these results advance the study of cysteine oxidation in biological systems.
- Published
- 2019