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Norbornene Probes for the Detection of Cysteine Sulfenic Acid in Cells

Authors :
Alcock, Lisa J
Oliveira, Bruno L
Deery, Michael J
Pukala, Tara L
Perkins, Michael V
Bernardes, Gonçalo JL
Chalker, Justin M
Alcock, Lisa J [0000-0001-9068-0756]
Pukala, Tara L [0000-0001-7391-1436]
Bernardes, Gonçalo JL [0000-0001-6594-8917]
Chalker, Justin M [0000-0002-7504-5508]
Apollo - University of Cambridge Repository
Publication Year :
2019
Publisher :
American Chemical Society (ACS), 2019.

Abstract

Norbornene derivatives were validated as probes for cysteine sulfenic acid on proteins and in live cells. Trapping sulfenic acids with norbornene probes is highly selective and revealed a different reactivity profile than the traditional dimedone reagent. The norbornene probe also revealed a superior chemoselectivity when compared to a commonly used dimedone probe. Together, these results advance the study of cysteine oxidation in biological systems.

Details

Database :
OpenAIRE
Accession number :
edsair.od.......109..e7d8fbdb975e076036ebc6dfa122cc12