5 results on '"Céline Margot"'
Search Results
2. Bacteria‐induced production of the antibacterial sesquiterpene lagopodin B in Coprinopsis cinerea
- Author
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Markus Künzler, Claire E. Stanley, Brandon I. Morinaka, Anja Kombrink, Andrew J. deMello, Céline Margot, Ramon Sieber, Jörn Piel, Martina Stöckli, and Gerald Lackner
- Subjects
0303 health sciences ,biology ,Hypha ,030306 microbiology ,fungi ,Antibiosis ,Bacillus subtilis ,biology.organism_classification ,Microbiology ,Anti-Bacterial Agents ,Fungal Proteins ,Gene product ,03 medical and health sciences ,Coprinopsis cinerea ,Cytochrome P-450 Enzyme System ,Gram-Negative Bacteria ,Agaricales ,Axenic ,Sesquiterpenes ,Molecular Biology ,Mycelium ,Bacteria ,030304 developmental biology - Abstract
Fungi defend their ecological niche against antagonists by producing antibiosis molecules. Some of these molecules are only produced upon confrontation with the antagonist. The basidiomycete Coprinopsis cinerea induces the expression of the sesquiterpene synthase-encoding gene cop6 and its two neighboring genes coding for cytochrome P450 monooxygenases in response to bacteria. We further investigated this regulation of cop6 and examined if the gene product is involved in the production of antibacterials. Cell-free supernatants of axenic cultures of the Gram-positive bacterium Bacillus subtilis were sufficient to induce cop6 transcription assessed using a fluorescent reporter strain. Use of this strain in a microfluidic device revealed that the cop6 gene was induced in all hyphae directly exposed to the supernatant and that induction occurred within less than one hour. Targeted replacement of the cop6 gene demonstrated the requirement of the encoded synthase for the biosynthesis of the sesquiterpene lagopodin B, a previously reported antibacterial compound from related species. Accordingly, lagopodin B from C. cinerea inhibited the growth of several Gram-positive bacteria including B. subtilis but not Gram-negative bacteria. Our results demonstrate that the C. cinerea vegetative mycelium responds to soluble compounds of a bacterial culture supernatant by local production of an antibacterial secondary metabolite.
- Published
- 2019
3. Import of Aspartate and Malate by DcuABC Drives H2/Fumarate Respiration to Promote Initial Salmonella Gut-Lumen Colonization in Mice
- Author
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Rebekka Bauer, Ersin Gül, Beat Christen, V Miguelangel Cuenca, Shinichi Sunagawa, Leanid Laganenka, Franziska Besser, Julia A. Vorholt, Laura Heeb, Steffen Porwollik, Philipp J. Keller, Bidong D. Nguyen, Joel Rüthi, Susanne Meile, Johannes Hartl, Lea Fuchs, Michael McClelland, Pau Pérez Escriva, Wolf-Dietrich Hardt, Matthias Christen, Uwe Sauer, Céline Margot, Céline Fetz, and Markus Furter
- Subjects
Salmonella typhimurium ,Male ,Salmonella ,Antiporter ,Malates ,Succinic Acid ,medicine.disease_cause ,Inbred C57BL ,Feces ,Mice ,0302 clinical medicine ,Fumarates ,Colonization ,chemistry.chemical_classification ,0303 health sciences ,Electron acceptor ,Intestines ,Infectious Diseases ,Medical Microbiology ,Administration ,Female ,Sequence Analysis ,Oral ,16S ,Anaerobic respiration ,mouse model ,Citric Acid Cycle ,Immunology ,Biology ,Microbiology ,03 medical and health sciences ,Metabolism ,Intestine ,Infection ,Mouse model ,Bacterial Proteins ,Virology ,Respiration ,medicine ,Escherichia coli ,Animals ,intestine ,030304 developmental biology ,Ribosomal ,Aspartic Acid ,Animal ,DNA ,infection ,Gastrointestinal Microbiome ,Enzyme ,Emerging Infectious Diseases ,chemistry ,Mutagenesis ,Disease Models ,RNA ,Parasitology ,Digestive Diseases ,metabolism ,030217 neurology & neurosurgery - Abstract
Summary Initial enteropathogen growth in the microbiota-colonized gut is poorly understood. Salmonella Typhimurium is metabolically adaptable and can harvest energy by anaerobic respiration using microbiota-derived hydrogen (H2) as an electron donor and fumarate as an electron acceptor. As fumarate is scarce in the gut, the source of this electron acceptor is unclear. Here, transposon sequencing analysis along the colonization trajectory of S. Typhimurium implicates the C4-dicarboxylate antiporter DcuABC in early murine gut colonization. In competitive colonization assays, DcuABC and enzymes that convert the C4-dicarboxylates aspartate and malate into fumarate (AspA, FumABC), are required for fumarate/H2-dependent initial growth. Thus, S. Typhimurium obtains fumarate by DcuABC-mediated import and conversion of L-malate and L-aspartate. Fumarate reduction yields succinate, which is exported by DcuABC in exchange for L-aspartate and L-malate. This cycle allows S. Typhimurium to harvest energy by H2/fumarate respiration in the microbiota-colonized gut. This strategy may also be relevant for commensal E. coli diminishing the S. Typhimurium infection.
- Published
- 2020
4. Import of Aspartate and Malate by DcuABC Drives H
- Author
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Bidong D, Nguyen, Miguelangel, Cuenca V, Johannes, Hartl, Ersin, Gül, Rebekka, Bauer, Susanne, Meile, Joel, Rüthi, Céline, Margot, Laura, Heeb, Franziska, Besser, Pau Pérez, Escriva, Céline, Fetz, Markus, Furter, Leanid, Laganenka, Philipp, Keller, Lea, Fuchs, Matthias, Christen, Steffen, Porwollik, Michael, McClelland, Julia A, Vorholt, Uwe, Sauer, Shinichi, Sunagawa, Beat, Christen, and Wolf-Dietrich, Hardt
- Subjects
Male ,Salmonella typhimurium ,Aspartic Acid ,Citric Acid Cycle ,Malates ,Succinic Acid ,Administration, Oral ,Sequence Analysis, DNA ,Gastrointestinal Microbiome ,Intestines ,Mice, Inbred C57BL ,Disease Models, Animal ,Feces ,Mice ,Bacterial Proteins ,Fumarates ,Mutagenesis ,Salmonella ,RNA, Ribosomal, 16S ,Escherichia coli ,Animals ,Female - Abstract
Initial enteropathogen growth in the microbiota-colonized gut is poorly understood. Salmonella Typhimurium is metabolically adaptable and can harvest energy by anaerobic respiration using microbiota-derived hydrogen (H
- Published
- 2019
5. Import of Aspartate and Malate by DcuABC Drives H 2/Fumarate Respiration to Promote Salmonella Gut-Luminal Colonization
- Author
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Matthias Christen, Julia A. Vorholt, Laura Heeb, V Miguelangel Cuenca, Bidong D. Nguyen, Michael McClelland, Pau Pérez Escriva, Susanne Meile, Uwe Sauer, Céline Fetz, Steffen Porwollik, Markus Furter, Céline Margot, Rebekka Bauer, Franziska Besser, Shinichi Sunagawa, Beat Christen, Joel Rüthi, Johannes Hartl, and Wolf-Dietrich Hardt
- Subjects
chemistry.chemical_classification ,Salmonella ,Anaerobic respiration ,Chemistry ,Electron donor ,Metabolism ,Electron acceptor ,medicine.disease_cause ,chemistry.chemical_compound ,Enzyme ,Biochemistry ,Respiration ,medicine ,Gene - Abstract
Enteropathogen growth in the microbiota-colonized gut is poorly understood. Salmonella Typhimurium is metabolically plastic and can harvest energy by anaerobic respiration using hydrogen (H2), a product of microbiota metabolism, as an electron donor and fumarate as an electron acceptor. As fumarate is scarce in the gut, the source of this electron acceptor is unclear. Transposon sequencing analysis along the colonization-trajectory of S.Typhimurium implicated the C4-dicarboxylate exchanger DcuABC in murine gut colonization. In competitive colonization assays, DcuABC and enzymes converting the C4-dicarboxylates aspartate and malate into fumarate (AspA, FumABC), were required for fumarate/H2-dependent growth. Thus, S.Typhimurium obtains fumarate by DcuABC-mediated import and the subsequent conversion of L-malate and L-aspartate into fumarate. Fumarate reduction yields succinate, which is exported by DcuABC in exchange for L-aspartate and L-malate. This mechanism allows S.Typhimurium to harvest energy by H2/fumarate respiration in the microbiota-colonized gut. It might be relevant for other enteropathogens encoding the requisite genes.
- Published
- 2019
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