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Bacteria‐induced production of the antibacterial sesquiterpene lagopodin B in Coprinopsis cinerea
- Source :
- Molecular Microbiology. 112:605-619
- Publication Year :
- 2019
- Publisher :
- Wiley, 2019.
-
Abstract
- Fungi defend their ecological niche against antagonists by producing antibiosis molecules. Some of these molecules are only produced upon confrontation with the antagonist. The basidiomycete Coprinopsis cinerea induces the expression of the sesquiterpene synthase-encoding gene cop6 and its two neighboring genes coding for cytochrome P450 monooxygenases in response to bacteria. We further investigated this regulation of cop6 and examined if the gene product is involved in the production of antibacterials. Cell-free supernatants of axenic cultures of the Gram-positive bacterium Bacillus subtilis were sufficient to induce cop6 transcription assessed using a fluorescent reporter strain. Use of this strain in a microfluidic device revealed that the cop6 gene was induced in all hyphae directly exposed to the supernatant and that induction occurred within less than one hour. Targeted replacement of the cop6 gene demonstrated the requirement of the encoded synthase for the biosynthesis of the sesquiterpene lagopodin B, a previously reported antibacterial compound from related species. Accordingly, lagopodin B from C. cinerea inhibited the growth of several Gram-positive bacteria including B. subtilis but not Gram-negative bacteria. Our results demonstrate that the C. cinerea vegetative mycelium responds to soluble compounds of a bacterial culture supernatant by local production of an antibacterial secondary metabolite.
- Subjects :
- 0303 health sciences
biology
Hypha
030306 microbiology
fungi
Antibiosis
Bacillus subtilis
biology.organism_classification
Microbiology
Anti-Bacterial Agents
Fungal Proteins
Gene product
03 medical and health sciences
Coprinopsis cinerea
Cytochrome P-450 Enzyme System
Gram-Negative Bacteria
Agaricales
Axenic
Sesquiterpenes
Molecular Biology
Mycelium
Bacteria
030304 developmental biology
Subjects
Details
- ISSN :
- 13652958 and 0950382X
- Volume :
- 112
- Database :
- OpenAIRE
- Journal :
- Molecular Microbiology
- Accession number :
- edsair.doi.dedup.....98138d497e4c1a2edc09111ca3e4af6d