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Anaerobic digestion of organic waste results in production of biogas and a nutrient-rich digestate that has an established use as fertilizer in plant production. This study evaluated use of anaerobic digestate based on a high concentration of organic household waste as a fertilizer in sawdust-based production of oyster mushrooms (Pleurotus ostreatus). Inclusion of 0.5 L of anaerobic digestate (AD) per kg sawdust gave similar productivity in terms of biological efficiency (79.5 +/- 5.4 %), and protein concentration (24.7 +/- 2.4 % of dry weight (dw)) as standard mushroom substrate (78.1 +/- 5.3 %, and 21.9 +/- 3.0 % of dw, respectively). However, mushroom growth was impaired at the highest concentration of anaerobic digestate tested, 1 L digestate per kg dw sawdust. Comparison of the AD-fertilized substrate with a mushroom substrate with standard components (sawdust, wheat bran, calcium sulfate) and with similar C/N-ratio revealed some differences in elemental composition of the fruiting bodies, with an major increase in sodium concentration for the AD-fertilized substrate compared with the standard substrate (413.3 +/- 28.9 and 226.7 +/- 30.6 mg kg(-1) dw, respectively). This difference can be explained by high sodium concentration in the anaerobic digestate, most likely due to inclusion of food scraps from households and restaurants in the biodigester feedstock. Screening of both substrates for a total of 133 micro -pollutants revealed that total sum of micropollutants was significantly higher in the AD-fertilized substrate (258 +/- 12 ng/g dw substrate) than in the standard substrate (191 +/- 35 ng/g dw substrate). Nitrogen losses during preparation of the AD-fertilized substrate were negligible.

Rheology and the study of viscoelastic materials are an integral part of engineering and the study of biophysical systems. Tissue rheology is even used in the study of cancer and other diseases. However, the cost of a rheometer is feasible only for colleges, universities, and research laboratories. Even if a rheometer can be purchased, it is bulky and delicately calibrated, limiting its usefulness to the laboratory itself. The design presented here is less than a tenth of the cost of a professional rheometer. The design is also portable, making it the ideal solution to introduce viscoelasticity to high school students as well as for use in the field for obtaining rheological data.

Novel tests of semantic memory (SM)—for example, memory for news events (NE; news facts) or famous personalities—are useful for estimating the severity of retrograde amnesia. Individuals with mild cognitive impairment exhibit relatively intact SM/language on traditional neuropsychological tests but exhibit consistent impairment on novel tests of SM, suggesting novel SM tests are dissimilar from traditional SM tests. To identify the relationship between NE memory and traditional cognitive measures, older adults (N = 51) completed a traditional neuropsychological battery and the Retrograde Memory News Events Test (RM-NET; a new test that robustly measures NE memory across the adult life span with high temporal resolution), and the relationship between performance on these tests was examined. Total RM-NET scores were more closely aligned with episodic memory scores than SM scores. The strength of the association between NE scores and episodic memory scores decreased as the age of NE memory increased. Tests of news events appear to reflect performance on traditional tests of episodic memory rather than SM, especially when recent news events are tested.

Funder: Albert-Ludwigs-Universität Freiburg im Breisgau (1016), The aim of this study is to understand the strength behaviour and fragment size of rocks during indirect, quasi-static and dynamic tensile tests. Four rocks with different lithological characteristics, namely: basalt, granite, sandstone, and marble were selected for this study. Brazilian disc experiments were performed over a range of strain rates from ~ 10–5 /s to 2.7 × 101 /s using a hydraulic loading frame and a split Hopkinson bar. Over the range of strain rates, our measurements of dynamic strength increase are in good agreement with the universal theoretical scaling relationship of (Kimberley et al., Acta Mater 61:3509–3521, 2013). Dynamic fragmentation during split tension mode failure has received little attention, and in the present study, we determine the fragment size distribution based on the experimentally fragmented specimens. The fragments fall into two distinct groups based on the nature of failure: coarser primary fragments, and finer secondary fragments. The degree of fragmentation is assessed in terms of characteristic strain rate and is compared with existing theoretical tensile fragmentation models. The average size of the secondary fragments has a strong strain rate dependency over the entire testing range, while the primary fragment size is less sensitive at lower strain rates. Marble and sandstone are found to generate more pulverised secondary debris when compared to basalt and granite. Furthermore, the mean fragment sizes of primary and secondary fragments are well described by a power-law function of strain rate.

INTRODUCTION: Alcohol consumption during pregnancy is related to severe birth complications such as low birthweight, preterm birth and birth defects. During the last decade, the Alcohol Use Disorders Identification Test (AUDIT) has been used as a screening tool in Swedish maternal healthcare units to identify hazardous, pre-pregnancy alcohol use. However, evaluation of the screening with AUDIT, as well as adverse maternal or neonatal outcomes, has not been assessed at a national level. MATERIAL AND METHODS: This was a population-based cohort study of 530 458 births from 2013 to 2018 using demographic, reproductive and maternal health data from the Swedish Pregnancy Register. Self-reported alcohol consumption in the year before pregnancy, measured as AUDIT scores, was categorized into moderate (6-13 points) and high-risk (14-40 points) consumption, with low-risk (0-5 points) consumption as the reference group. Associations with pregnancy- and birth outcomes were explored with logistic regressions using generalized estimating equation models, adjusting for maternal and socioeconomic characteristics. Estimates are presented as adjusted odds ratios (aORs) with 95% confidence intervals (CIs). RESULTS: High-risk and moderate pre-pregnancy alcohol consumption was associated with preeclampsia, preterm birth and birth of an infant small for gestational age (SGA), but these associations were nonsignificant after adjustments. Prior moderate-risk (aOR 1.29, 95% CI 1.17-1.42) and high-risk consumption (aOR 1.62, 95% CI 1.17-2.25) increased the likelihood of intrapartum and neonatal infections. CONCLUSIONS: Apart from identifying hazardous alcohol consumption prior to pregnancy and the offer of counseling, screening with the AUDIT in early pregnancy indicates a high risk of inflammatory-/placenta-mediated pregnancy and birth outcomes. For most outcomes, AUDIT was not an independent contributor when adjusting for confounding factors. Hazardous alcohol use prior to pregnancy was independently linked to intrapartum and neonatal infections; conditions associated with morbidity and long-term sequalae. These associations may be explained by alcohol-induced changes in the maternal or fetal immune system in early pregnancy or persistent alcohol intake during pregnancy, or may depend on unidentified confounding factors.

Survival has frequently been studied and reported in patients with long term intestinal failure (IF). However, studies comparing the survival and mortality rates with the background population are rare. This study compares the survival in an adult IF, non-malignant, short bowel syndrome (SBS) cohort with a control group and with age- and sex-specific background mortality rates.Patients with SBS, defined by a small bowel length of 200 cm or less, due to non-malignant disease, were included and followed until death or censoring on 31 December 2017. Causes of deaths occurring during home parenteral support (HPS) were assessed by review of the charts. Each case was matched with ten controls from the background population according to year of HPS initiation, age, and sex, and their survival was compared. Furthermore, age- and sex-specific mortality rates of the background population were used to calculate the standardized mortality ratio (SMR) and excess mortality.After five years, patients who initiated HPS had a relative survival of 76%. The SMR was 5.0 and the excess mortality was 50 per 1000 years. HPS-related deaths were assessed to account for 11% of deaths during HPS and occurred with an incidence of 10 per 1000 years. The excess mortality was as low as 15 per 1000 years in cases aged less than 40 years. Patients weaning off HPS had a mortality rate closer to that expected in the background population.In adult patients with a non-malignant cause of SBS-IF, the excess mortality was 50 per 1000 years. However, HPS related deaths were rarely registered with an incidence of 10 HPS related deaths per 1000 HPS treatment years.

The central hypothesis of the genotype–phenotype relationship is that the phenotype of a developing organism (i.e., its set of observable attributes) depends on its genome and the environment. However, as we learn more about the genetics and biochemistry of living systems, our understanding does not fully extend to the complex multiscale nature of how cells move, interact, and organize; this gap in understanding is referred to as the genotype-to-phenotype problem. The physics of soft matter sets the background on which living organisms evolved, and the cell environment is a strong determinant of cell phenotype. This inevitably leads to challenges as the full function of many genes, and the diversity of cellular behaviors cannot be assessed without wide screens of environmental conditions. Cellular mechanobiology is an emerging field that provides methodologies to understand how cells integrate chemical and physical environmental stress and signals, and how they are transduced to control cell function. Biofilm forming bacteria represent an attractive model because they are fast growing, genetically malleable and can display sophisticated self-organizing developmental behaviors similar to those found in higher organisms. Here, we propose mechanobiology as a new area of study in prokaryotic systems and describe its potential for unveiling new links between an organism's genome and phenome.

An upper GI endoscopy or EGD (esophagogastroduodenoscopy) is a procedure to diagnose and treat problems in your upper GI (gastrointestinal) tract. The upper GI tract includes your food pipe (esophagus), stomach, and the first part of your small intestine (the duodenum). This procedure is done using a long, flexible tube called an endoscope. The tube has a tiny light and video camera on one end. The tube is put into your mouth and throat. Then it is slowly pushed through your esophagus and stomach, and into your duodenum. Video images from the tube are seen on a monitor. You may have an upper GI endoscopy as an outpatient or as part of your stay in a hospital. After the procedure, you will be taken to the recovery room to be watched. Once your blood pressure, pulse, and breathing are stable and you are awake and alert, you will be taken to your hospital room. Or you may be discharged to your home. If you are going home, someone must drive you. You will not be allowed to eat or drink anything until your gag reflex returns. This is to prevent you from choking. You may have a sore throat and pain for a few days when you swallow. This is normal. You may go back to your normal diet and activities, unless you have other instructions.Call your healthcare provider if you have any of the following:Fever or chillsRedness, swelling, or bleeding or other drainage from the IV siteBelly pain, nausea, or vomitingBlack, tarry, or bloody stoolsTroubleswallowingThroat or chest pain that gets worseyour healthcare provider may give you other instructions, depending on your situation.

Objectives Sleep enhances the consolidation of memories. Here, we investigated whether sleep-dependent memory consolidation differs between healthy subjects and narcolepsy type 1 (NT1) patients. Material and Methods We recruited 18 patients with NT1 and 24 healthy controls. The consolidation of spatial (declarative memory; 2-dimensional object location) and procedural (non-declarative memory; finger sequence tapping) memories was examined across one night of at-home sleep. Sleep was measured by an ambulatory sleep recording device. Results The overnight gain in the number of correctly recalled sequences in the finger-tapping test was smaller for NT1 patients than healthy subjects (+8.1% vs. +23.8% from pre-sleep learning to post-sleep recall, p = .035). No significant group differences were found for the overnight consolidation of spatial memory. Compared to healthy subjects, the sleep of NT1 patients was significantly more fragmented and shallow. However, no significant correlations were found between sleep parameters and overnight performance changes on the memory tests in the whole group. Conclusion The sleep-dependent consolidation of procedural but not spatial memories may be impaired among patients with NT1. Therefore, future studies are warranted to examine whether sleep improvement, for example, using sodium oxybate, can aid the sleep-dependent formation of procedural memories among NT1 patients.

Background: Overweight in early childhood often tracks into adolescence and adulthood and early childhood is a critical period for developing sustained overweight. This study aims to investigate the early detection of childhood overweight (including obesity) and related cardiometabolic complications in a Danish population-based cohort of children aged 2.5–8 years in collaboration with primary care municipal dental clinics and public health nurses. Methods: In this prospective population-based cohort study, 335 pre-school children (age 2.5 and 5 years) were recruited from municipal dental clinics, and 657 school children (age 6–8 years) by public health nurses. A subgroup of 392 children (40%) participated in additional hospital-based examinations including blood pressure measurement and a blood sample. Children were re-examined approximately one year later. Results: The prevalence of overweight was 13.73% in pre-school children and 13.69% in school children at baseline. In the pre-school children, differences in cardiometabolic risk markers between children with and without overweight were minor, whereas in school children with overweight, cardiometabolic derangements were manifest including significantly higher levels of fasting glucose, insulin, homoeostasis model of assessment for insulin resistance, triglycerides, and alanine aminotransferase and lower levels of high-density lipoprotein cholesterol. During follow-up the prevalence of overweight did not change in pre-school children but increased to 17.0% in school children. Conclusions: Existing contacts with the primary health care sector, including dental care, can successfully be used for detection of overweight. This study suggests that early detection should be initiated at pre-school ages since overweight-related complications are already established by school ages.

Background: Nursing faculties' perceptions influence nursing education. This article describes how nursing faculty perceive nursing students' development of professional competence by integrating theory and practice, and by examining how this context influences this integration. Method: With a phenomenographic approach, interviews were conducted with nine participants and analyzed into qualitatively different categories of description. Results: The integration of theoretical and practical knowledge is perceived as a challenge or as naturally intertwined in the entire education, focusing mainly on individual prerequisites for learning. Perceptions of a common mission create coherence for students. Evident learning structures are needed, and there is a perceived discrepancy between nurses' competence and expectations in health care. Conclusion: If the theory and practice gap could be perceived as a driving force for continuous learning, it could become an incentive for closer collaboration by creating structures for competence development that include individuals, teams, and organizations. [ J Nurs Educ . 2022;61(5):236–241.]

Purpose Epithelial ovarian cancer is usually diagnosed in the advanced stages. To choose the best therapeutic approach, an accurate preoperative assessment of the tumour extent is crucial. This study aimed to determine whether the peritoneal cancer index (PCI), the amount of ascites, and the presence of cardiophrenic nodes (CPLNs) visualized by computed tomography (CT) can assess the tumour extent (S-PCI) and residual disease (RD) for advanced ovarian cancer (AOC) patients treated with upfront surgery. Methods In total, 118 AOC cases were included between January 2016 and December 2018 at Skåne University Hospital, Lund, Sweden. Linear regression and interclass correlation (ICC) analyses were used to determine the relationship between CT-PCI and S-PCI. The patients were stratified in complete cytoreductive surgery (CCS) with no RD or to non-CCS with RD of any size. The amount of ascites on CT (CT-ascites), CA-125 and the presence of radiological enlarged CPLNs (CT-CPLN) were analysed to evaluate their impact on estimating RD. Results CT-PCI correlated well with S-PCI (0.397; 95% CI 0.252–0.541; p p p = 0.000). The sensitivity, specificity, positive predictive value and negative predictive value were 58.5, 70.3, 52.2 and 75.4%, respectively. CT-ascites above 1000 ml predicted RD (OR 3.510 (1.298–9.491) p Conclusion CT is a reliable tool to assess the extent of the disease in advanced ovarian cancer. Higher CT-PCI scores and large volumes of ascites estimated on CT predicted RD of any size.

This paper investigates least squares spectral analysis as a tool to analyze non-stationary signals from pass-by noise measurements in water. The spectral analysis involves successive least squares fitting of a finite Fourier series to approximate the observation in a piecewise manner. The least squares spectral analysis is used to search the signals for first- and second-order periodicity as well as the presence of fundamental periodicity. A first-order analysis reveals line components in the signals, whereas a second-order analysis reveals periodic amplitude modulations. Analysis with a higher-order finite Fourier series reveals harmonic structures in the signals. The main contribution of this paper is the model of a magnitude-squared cosine wave which can be used to analyze second-order periodicity. The developed short-time least squares spectral analysis is illustrated on noise radiated from a rigid inflatable boat in shallow water.

Background Imaging ovarian cancer (OC) includes evaluating peritoneal carcinomatosis (PC) and enlarged cardio phrenic lymph nodes (CPLN) by computed tomography (CT), and thorough evaluation is tedious work. A “CT short score” with high-risk CT parameters might be a more pragmatic approach, but it is not known if such a short score associates with aggressive OC subtypes and impaired OC survival. Further, it is not known if certain established OC risk factors are linked to high-risk CT-findings which would be important in image evaluation. Herein, we investigate a CT short score and its relation to baseline characteristics, OC subtypes, and survival. Methods The Malmö Diet and Cancer Study is a prospective cohort that included 17,035 women (1991–1996). Baseline characteristics and tumor information on 159 OC and information on OC specific survival (last follow-up, 2017-12-31) was registered. A CT short score (CPLN and PC-index (PCI) in seven regions) was registered and associations with clinical stage [stage I vs. advanced stage (II-IV), histological type/grade (high grade serous and endometrioid vs. other subtypes], and OC-specific survival were analyzed with logistic and Cox regression, respectively. Parity and menopausal status were analyzed in relation to short score and PCI. Results There was an association between higher short score and advanced clinical stage (adjusted OR 2.76 (1.42—5.38)), adjusted for age at diagnosis and histological type/grade. Higher short score was associated with impaired OC specific survival (adjusted HR 1.17 (1.01—1.35)), adjusted for age at diagnosis, histological type/grade, and clinical stage. There were no significant associations between parity, menopausal status, and short score/PCI. Conclusions CT short score was significantly associated with advanced clinical stages and impaired OC survival. A pragmatic approach (based on CT) to evaluate high risk image findings in OC could help reduce radiologists’ workload and at the same time provide structured reports to surgeons and oncologists involved in OC care.

Supplementary Data from Orthovoltage X-Rays Exhibit Increased Efficacy Compared with γ-Rays in Preclinical Irradiation

Radionuclide irradiators (137Cs and 60Co) are commonly used in preclinical studies ranging from cancer therapy to stem cell biology. Amidst concerns of radiological terrorism, there are institutional initiatives to replace radionuclide sources with lower energy X-ray sources. As researchers transition, questions remain regarding whether the biological effects of γ-rays may be recapitulated with orthovoltage X-rays because different energies may induce divergent biological effects. We therefore sought to compare the effects of orthovoltage X-rays with 1-mm Cu or Thoraeus filtration and 137Cs γ-rays using mouse models of acute radiation syndrome. Following whole-body irradiation, 30-day overall survival was assessed, and the lethal dose to provoke 50% mortality within 30-days (LD50) was calculated by logistic regression. LD50 doses were 6.7 Gy, 7.4 Gy, and 8.1 Gy with 1-mm Cu-filtered X-rays, Thoraeus-filtered X-rays, and 137Cs γ-rays, respectively. Comparison of bone marrow, spleen, and intestinal tissue from mice irradiated with equivalent doses indicated that injury was most severe with 1-mm Cu-filtered X-rays, which resulted in the greatest reduction in bone marrow cellularity, hematopoietic stem and progenitor populations, intestinal crypts, and OLFM4+ intestinal stem cells. Thoraeus-filtered X-rays provoked an intermediate phenotype, with 137Cs showing the least damage. This study reveals a dichotomy between physical dose and biological effect as researchers transition to orthovoltage X-rays. With decreasing energy, there is increasing hematopoietic and intestinal injury, necessitating dose reduction to achieve comparable biological effects.Significance:Understanding the significance of physical dose delivered using energetically different methods of radiation treatment will aid the transition from radionuclide γ-irradiators to orthovoltage X-irradiators.

The levels of specific proteins in human blood are the most commonly used indicators of potential health-related problems1. Understanding the genetic and other determinants of the human plasma proteome can aid in biomarker research and drug development. Diverse factors including genetics, age, sex, body mass index (BMI), growth and development including puberty can affect the circulating levels of proteins2–5. Affinity-based proteomics can infer the relationship between blood protein levels and these factors at a large scale6–10. Compared to these methods, mass spectrometry (MS)-based proteomics provides much higher specificity of identification and quantification11–13, but existing studies are limited by small sample sizes or low numbers of quantified proteins14–17. Here we aim to elucidate to which extent genomic variation affects plasma protein levels across diverse age ranges and cohort characteristics. Employing a streamlined and highly quantitative MS-based plasma proteomics workflow, we measured the plasma proteome of 2,147 children and adolescents. Levels of 90% of these proteins were significantly associated with age, sex, BMI or genetics. More than 1,000 protein quantitative trait loci (pQTLs) – a third of which were novel – regulated protein levels between a few percent and up to 30-fold. These replicated excellently in an independent cohort of 558 adults, with highly concordant effect sizes (Pearson’s r > 0.97). We developed a framework to eliminate artefactual pQTLs due to protein-altering variants, paving the way for large-scale interrogation of pQTLs using MS-based proteomics. Our data reveal unexpectedly extensive genetic impacts on plasma protein levels, consistent from childhood into adulthood. These findings have implications for biomarker research and drug development.HighlightsFirst large-scale proteome-wide and genome-wide association study in children and adolescentsMS-based proteomics achieves very high specificity and quantitative accuracyRobust plasma protein trajectories during development predict age and body mass indexLargest set of pQTLs for plasma proteome by MS-based proteomicspQTLs are highly replicable between children and adultsLarge-scale pQTL identification enables generic drug target validation

This article presents an interactive method for 3D cloud animation at the landscape scale by employing machine learning. To this end, we utilize deep convolutional generative adversarial network (DCGAN) on GPU for training on home-captured cloud videos and producing coherent animation frames. We limit the size of input images provided to DCGAN, thereby reducing the training time and yet producing detailed 3D animation frames. This is made possible through our preprocessing of the source videos, wherein several corrections are applied to the extracted frames to provide an adequate input training data set to DCGAN. A significant advantage of the presented cloud animation is that it does not require any underlying physics simulation. We present detailed results of our approach and verify its effectiveness using human perceptual evaluation. Our results indicate that the proposed method is capable of convincingly realistic 3D cloud animation, as perceived by the participants, without introducing too much computational overhead.

The genotype-to-phenotype problem (G2P) for multicellular development asks how genetic inputs control collective phenotypic outputs. It is a difficult problem even to observe. On the genotype side, the phenotypic impact of mutation is often subtle due at least partly to gene redundancy and myriad other factors. On the phenotype side, biological and even technical developmental replicates can display significant phenotypic variation due at least in part to stochasticity, again with other factors. We attempt to partially resolve the G2P inputs and outputs from the obfuscating effects of factors like redundancy and stochasticity. As a model organism, we selected the biofilm-forming speciesMyxococcus xanthus, a motile self-organizing bacterium that forms three-dimensional cell aggregates that grow and mature into spore-filled fruiting bodies when under starvation stress. We developed data acquisition tools and analysis and visualization methods that can produce a topological map ofM. xanthusdevelopment. We demonstrate that even subtle effects on developmental dynamics caused by mutation can be identified, discriminated, characterized, and given statistical significance.

The nucleus accumbens shell (NAcSh) is critically important for reward valuations, yet it remains unclear how valuation information is integrated in this region to drive behaviour during reinforcement learning. Using an optogenetic spatial self-stimulation task in mice, here we show that contingent activation of different excitatory inputs to the NAcSh change expression of different reward-related behaviours. Our data indicate that medial prefrontal inputs support place preference via repeated actions, ventral hippocampal inputs consistently promote place preferences, basolateral amygdala inputs produce modest place preferences but as a byproduct of increased sensitivity to time investments, and paraventricular inputs reduce place preferences yet do not produce full avoidance behaviour. These findings suggest that each excitatory input provides distinct information to the NAcSh, and we propose that this reflects the reinforcement of different credit assignment functions. Our finding of a quadruple dissociation of NAcSh input-specific behaviours provides insights into how types of information carried by distinct inputs to the NAcSh could be integrated to help drive reinforcement learning and situationally appropriate behavioural responses.

The lung contains numerous specialized cell types with distinct roles in tissue function and integrity. To clarify the origins and mechanisms generating cell heterogeneity, we created a comprehensive topographic atlas of early human lung development. Here we report 83 cell states and several spatially resolved developmental trajectories and predict cell interactions within defined tissue niches. We integrated single-cell RNA sequencing and spatially resolved transcriptomics into a web-based, open platform for interactive exploration. We show distinct gene expression programmes, accompanying sequential events of cell differentiation and maturation of the secretory and neuroendocrine cell types in proximal epithelium. We define the origin of airway fibroblasts associated with airway smooth muscle in bronchovascular bundles and describe a trajectory of Schwann cell progenitors to intrinsic parasympathetic neurons controlling bronchoconstriction. Our atlas provides a rich resource for further research and a reference for defining deviations from homeostatic and repair mechanisms leading to pulmonary diseases.

This thesis describes studies into the synthesis, characterisation and reactivity of samarium(II) and samarium(III), europium(II) and ytterbium(II) complexes derived from the modified porphyrinogens \\(trans-N,N'\\)-dimethyl-meso-octaethylporphyrinogen, Et\\(_8\\)N\\(_4\\)Me\\(_2\\)H\\(_2\\), and \\(trans\\)-calix[2]benzene[2]pyrrole, Me\\(_8\\)N\\(_2\\)Ph\\(_2\\)H\\(_2\\). Chapter 2 is concerned with the synthesis of a new modified porphyrinogen \\(N,N'\\)-dimethyl-\\(meso\\)-octamethylporphyrinogen, Me\\(_8\\)N\\(_4\\)Me\\(_2\\)H\\(_2\\), via a convergent \3+1\" procedure from the condensation of 1-methy1-25-bis(11'-dimethylhydroxymethyl)pyrrole with 1-methy1-25-bis {(2'-pyrroly)dimethylmethyl}pyrrole in acetonitrile in the presence of scandium trifluoromethanesulfonate. The stepwise nature of the synthesis potentially allows independent functionalisation of various parts of the molecule. The unique electronic and steric properties of complexes derived from doubly deprotonated \\(NN'\\)-dimethyl-\\(meso\\)-octaethylporphyrinogen were exploited to force unusual reactivity and/or structural features in a range of lanthanide(II) and lanthanide(III) complexes. Chapter 3 details the synthesis of samarium(II) europium(II) and ytterbium(II) complexes of this macrocycle. Subsequent reaction with a range of 14-diazabuta-13-dienes (R-N=C(H)-C(H)=N-R R = \\(t\\)-Bu \\(i\\)-Pr and \\(n\\)-Bu) gave complexes featuring 14-diazabuta-13-diene binding to the lanthanide centres as neutral Lewis base donors chelating radical anions and bridging reduced dianions. Steric limitations were found to alter these structural outcomes and the complexes were characterised by X-ray crystal structure determination and NMR spectroscopy. Steric factors were also implicated in the observation of an unusual solvent mediated Sm(II)/Sm(III) reversibility in which a Sm(III) centre reverted to Sm(II) upon addition of coordinating solvent (R = \\(t\\)-Bu). Steric competition in the \\(NN'\\)-dimethyl-\\(meso\\)-octaethylporphyrinogen system was further examined in Chapter 4 by synthesis of a highly strained cyclopentadienyl Sm(III) complex [(Et\\(_8\\)N\\(_4\\)Me\\(_2\\))Sm(C\\(_5\\)H\\(_5\\))] featuring a major conformational deformation in the macrocycle. Also synthesised was a centrosymmetric bimetallic cyclooctatetraenediyl bound Sm(III) complex [{(Et\\(_8\\)N\\(_4\\)Me\\(_2\\))Sm}\\(_2\\)(¬¨¬µ:‚àÜvª\\(^2\\):‚àÜvª\\(^2\\)-COT)] in which the cyclooctatetraenediyl dianion is forced to adopt an ‚àÜvª\\(^2\\) -binding mode to each Sm centre. Solid state molecular structures of these strained molecules were complemented by \\(^1\\)H \\(^{13}\\)C 2D and variable temperature NMR studies of the cyclopentadienyl complex to examine fluctional processes in solution. Chapter 5 describes ligand substitution reactions in which Sm(III) complexes of 14-diazabuta-13-dienes were reacted with reducible substrates. Samarium(III) complexes of \\(t\\)-butyl- 14-diaza-1 3-diene and \\(i\\)-propyl-14-diaza-13-diene were found to reduce benzil to give the binuclear complex [{(Et\\(_8\\)N\\(_4\\)Me\\(_2\\))Sm}\\(_2\\){¬¨¬µ-OC(Ph)C(Ph)O}] with the concomitant formation of the free 14-diazabuta-13-diene. Also investigated was the highly strained [(Et\\(_8\\)N\\(_4\\)Me\\(_2\\))Sm(C\\(_5\\)H\\(_5\\))] which was found to react with 14-benzoquinone to give the binuclear complex [{(Et\\(_8\\)N\\(_4\\)Me\\(_2\\))Sm}\\(_2\\){¬¨¬µ-O(C\\(_6\\)H\\(_4\\))O}]. Chapter 6 describes the reductive chemistry of the Sm(II) complex [(Et\\(_8\\)N\\(_4\\)Me\\(_2\\))Sm(THF)\\(_2\\)]. It was found to reduce CO\\(_2\\) in a disproportionation reaction to give carbon monoxide and a bridging CO\\(_3\\)\\(^{2-}\\) moiety. The resulting binuclear samarium(III) complex was characterised by X-ray crystal structure determination and NMR spectroscopy. The Sm(II) complex was also used in redox transmetallation reactions with mercury thallium and silver salts. The reaction with silver tetrafluoroborate gave a Sm(III) tetrafluoroborate intermediate which underwent subsequent salt metathesis reactions with sodium cyclopentadienide and lithium iodide to give the respective samarium(III) derivatives. Chapter 7 details the synthesis of \\(trans\\)-calix[2]benzene[2]pyrrole by condensation of pyrrole with 13-bis(1'1'- dimethylhydroxymethyl)benzene in acetonitrile. The literature procedure for the synthesis of this macrocycle was improved by the use of a catalytic amount of scandium trifluoromethanesulfonate in place of stoichiometric boron trifluoride as Lewis acid. As a counterpoint to the conformationally restricted \\(NN'\\)-dimethyl-\\(meso\\)-octaethylporphyrinogen the less rigid doubly deprotonated \\(trans\\)-calix[2]benzene[2]pyrrole was investigated as a ligand for lanthanide metals. The potassium salt was synthesised by deprotonation of the neutral porphyrinogen with potassium metal. The lanthanide chemistry was investigated by reaction of the dipotassium salt with SmI\\(_2\\). The reaction was sensitive to conditions and resulted in mixtures from which a number of Sm(II) complexes featuring varying degrees of solvation and an unsolvated \"\\(N\\)-confused\" dimer were isolated. Molecular structures of the dipotassium salt mono- and bis-THF Sm(II) adducts and Sm(II) \\(N\\)-confused binuclear dimer were obtained. As derivatives a cationic Sm(III) cyclooctatetraenediyl complex and a potassium containing Sm(III) cyclooctatetraendiyl complex were obtained and characterised by X-ray crystal structure determination. Macrocyclic binding modes fell between the extremes of the samarium(II) mono-THF adduct (featuring a bis(‚àÜvª\\(^3\\)-arene) structural motif with only a slight metallocene bend angle ‚àÜvª\\(^5\\) -bound pyrrolide rings) and the cyclooctatetraenediyl Sm(III) complexes in which the macrocycle splays back to allow the large planar COT full access to the Sm coordination sphere resulting in an ‚àÜvª\\(^8\\) Sm-COT interaction and concomitant reduction in arene hapticity to a slipped ‚àÜvª\\(^1\\)-arrangement with pyrrolide rings ‚àÜvª\\(^1\\) -bound through the nitrogen. The Sm(II) complexes were also characterised by \\(^1\\)H NMR and/or variable temperature \\(^1\\)H NMR spectroscopy."

Understanding the mechanisms underlining forage production and its biomass nutritive quality at the omics level is crucial for boosting the output of high-quality dry matter per unit of land. Despite the advent of multiple omics integration for the study of biological systems in major crops, investigations on forage species are still scarce. Therefore, this study aimed to combine multi-omics from grass hybrids by prioritizing omic features based on the reconstruction of interacting networks and assessing their relevance in explaining economically important phenotypes. Transcriptomic and NMR-based metabolomic data were used for sparse estimation via the fused graphical lasso, followed by modularity-based gene expression and metabolite-metabolite network reconstruction, node hub identification, omic-phenotype association via pairwise fitting of a multivariate genomic model, and machine learning-based prediction study. Analyses were jointly performed across two data sets composed of family pools of hybrid ryegrass (Lolium perenne×L. multiflorum) andFestulolium loliaceum(L. perenne×Festuca pratensis), whose phenotypes were recorded for eight traits in field trials across two European countries in 2020/21. Our results suggest substantial changes in gene co-expression and metabolite-metabolite network topologies as a result of genetic perturbation by hybridizingL. perennewith another species within the genus relative to across genera. However, conserved hub genes and hub metabolomic features were detected between pedigree classes, some of which were highly heritable and displayed one or more significant edges with agronomic traits in a weighted omics-phenotype network. In spite of tagging relevant biological molecules as, for example, the light-induced rice 1 (LIR1), hub features were not necessarily better explanatory variables for omics-assisted prediction than features stochastically sampled. The use of the graphical lasso method for network reconstruction and identification of biological targets is discussed with an emphasis on forage grass breeding.

Self-incompatibility (SI) is a genetic mechanism of hermaphroditic plants to prevent inbreeding after self-pollination. Allogamous Poaceae species exhibit a unique gametophytic SI system controlled by two multi-allelic and independent loci, S and Z. Despite intense research efforts in the last decades, the genes that determine the initial recognition mechanism are yet to be identified. Here, we report the fine-mapping of the Z-locus in perennial ryegrass (Lolium perenne L.) and provide evidence that the pollen and stigma components are determined by two genes encoding DUF247 domain proteins (ZDUF247-I and ZDUF247-II) and the gene sZ, respectively. The pollen and stigma determinants are located side-by-side and were genetically linked in 10,245 individuals of two independent mapping populations segregating for Z. Moreover, they exhibited high allelic diversity as well as tissue-specific gene expression, matching the expected characteristics of SI determinants known from other systems. Revisiting the S-locus using the latest high-quality whole-genome assemblies revealed a similar gene composition and structure as found for Z, supporting the hypothesis of a duplicated origin of the two-locus SI system of grasses. Ultimately, comparative genomic analyses across a wide range of self-compatible and self-incompatible Poaceae species revealed that the absence of a functional copy of at least one of the six putative SI determinants is accompanied by a self-compatible phenotype. Our study provides new insights into the origin and evolution of the unique gametophytic SI system in one of the largest and economically most important plant families., Molecular Biology and Evolution, 40 (1), ISSN:0737-4038, ISSN:1537-1719

This qualitative study describes nurses' experiences and perceptions of how they develop the clinical gaze.This qualitative study used an inductive approach and content analysis to assess the experiences of newly graduated nurses, nurse managers, and nursing teachers. Nineteen interviews were conducted. To achieve credibility, the study followed the guidelines of the Consolidated Criteria for Reporting Qualitative research (COREQ).Two themes emerged: nurses' personal abilities and the learning culture. Learning culture was considered the foundation of the development of the clinical gaze. The clinical gaze was found to be developed in relationships with patients and when learning together with colleagues, in which the opportunities for reflection are central. To develop the clinical gaze, structures for learning activities, such as reflection, communication exercises, and simulation, are needed so that they become a natural part of daily work. This can also be achieved through supervision and skills training both at university and in a care context.Prerequisites for the development of the clinical gaze include physical presence with the patient combined with learning activities such as conscious reflection with others in a safe learning culture.

This integrative review aimed to examine empirical research on resilience among nursing students in the context of nursing education. Resilience helps nursing students handle challenges, such as changing learning styles and experiencing their first clinical practice.An integrative review.The search terms focused on resilience and health in nursing students and nursing education. The database used in this review were CINAHL Plus, PubMed and MEDLINE. The Mixed Methods Appraisal Tool appraised the studies' quality.This study explored 52 records and revealed three current research focuses related to nursing students' resilience: (1) the concept and description of resilience, (2) the characteristics affecting resilience and (3) the mediating role of resilience in maintaining holistic health. Recommendations include adding a resilience topic to the nursing curriculum, providing resilience enhancement programs, examining the relationship between resilience and holistic health and exploring the influence of resilience about global health crises.Resilience among nursing students plays a vital role in helping them to overcome adversities during their nursing education. Additionally, after graduation, nursing students can continue contributing to society as resilient Registered Nurses in the future.

Background and hypothesesImpaired interference control is a potential prognostic and endophenotypic marker of schizophrenia (SZ) and bipolar disorder (BP). Assessing children with familial high-risk (FHR) of SZ or BP enables characterization of early risk markers and we hypothesize that they express impaired interference control as well as aberrant brain activation compared to population-based control (PBC) children.Study designUsing a flanker task, we examined interference control together with functional magnetic resonance imaging (fMRI) in 11-to-12-year-old children with FHR of SZ (FHR-SZ) or FHR of BP (FHR-BP) and population-based control (PBC) children as part of a register-based, prospective cohort-study; The Danish High Risk and Resilience study – VIA 11.Study resultsWe included 85 (44 % female) FHR-SZ, 63 (52 % female) FHR-BP and 98 (50 % female) PBC in the analyses. Interference effects, caused by the spatial visuomotor conflict, showed no differences between groups. Bayesian ANOVA of reaction time (RT) variability, quantified by the coefficient of variation (CVRT), revealed a group effect with similarly higher CVRTin FHR-BP and FHR-SZ compared to PBC (BF10= 6.82). The fMRI analyses revealed no evidence for between-group differences in task-related brain activation. Post-hoc analyses excluding children with psychiatric illness yielded same results.ConclusionFHR-SZ and FHR-BP at age 11-to-12 show intact ability to resolve a spatial visuo-motor conflict and neural efficacy. The increased variability in RT may reflect difficulties in maintaining sustained attention. Since variability in RT was independent of existing psychiatric illness, it may reflect a potential endophenotypic marker of risk.

Fetal alcohol spectrum disorders (FASD) are neurodevelopmental/neurobehavioral conditions caused by prenatal alcohol exposure (PAE). Impairments caused by PAE contribute to the over-representation of individuals with FASD in the United States juvenile and adult criminal justice systems. These same impairments can equally impact on individuals with FASD who are witnesses to or victims of crime who also have to navigate the complexities of the criminal justice system. Difficulties include increased susceptibility to confabulation throughout the legal process that, in turn, can contribute to increased rates of poor outcomes including false confessions and wrongful convictions. Individuals with FASD are particularity at risk of confabulation when they are subjected to tactics, such as stressful and anxiety-provoking situations, threats, and leading, suggestive, or coercive questioning. Many professionals in the forensic context are unfamiliar with FASD or related confabulation risk and may unintentionally utilize tactics that intensify impacts of pre-existing impairment. This article serves as a beginner's guide for professionals working in criminal justice settings by (a) providing research-based overviews of FASD and confabulation, (b) describing how FASD may lead to confabulation, and (c) suggesting ways that professionals can modify protocols when interacting with individuals with FASD. Suggestions in this article hold the potential to decrease the risk of confabulation in the criminal justice system and decrease problematic outcomes, such as false confessions and wrongful convictions among individuals with FASD.

Cochlear implantation as a treatment for severe-to-profound hearing loss allows children to develop hearing, speech, and language in many cases. However, cochlear implants are generally provided beyond the infant period and outcomes are assessed after years of implant use, making comparison with normal development difficult. The aim was to study whether the rate of improvement of horizontal localization accuracy in children with bilateral implants is similar to children with normal hearing. A convenience sample of 20 children with a median age at simultaneous bilateral implantation = 0.58 years (0.42-2.3 years) participated in this cohort study. Longitudinal follow-up of sound localization accuracy for an average of ≈1 year generated 42 observations at a mean age = 1.5 years (0.58-3.6 years). The rate of development was compared to historical control groups including children with normal hearing and with relatively late bilateral implantation (≈4 years of age). There was a significant main effect of time with bilateral implants on localization accuracy (slope = 0.21/year, R

Joint modeling of correlated multi-environment and multi-harvest data of perennial crop species may offer advantages in prediction schemes and a better understanding of the underlying dynamics in space and time. The goal of the present study was to investigate the relevance of incorporating the longitudinal dimension of within-season multiple harvests of biomass yield and nutritive quality traits of forage perennial ryegrass (Lolium perenne L.) in a reaction norm model setup that additionally accounts for genotype-environment interactions. Genetic parameters and accuracy of genomic breeding value predictions were investigated by fitting three random regression (random coefficients) linear mixed models (gRRM) using Legendre polynomial functions to the data. All models accounted for heterogeneous residual variance and moving average-based spatial adjustments within environments. The plant material consisted of 381 bi-parental family pools and four check varieties of diploid perennial ryegrass evaluated in eight environments for biomass yield and nutritive quality traits. The longitudinal dimension of the data arose from multiple harvests performed four times annually. The specified design generated a total of 16,384 phenotypic data points for each trait. Genomic DNA sequencing was performed using DNA nanoball-based technology (DNBseq) and yielded 56,645 single nucleotide polymorphisms (SNPs) which were used to calculate the allele frequency-based genomic relationship matrix used in all genomic random regression models. Biomass yield’s estimated additive genetic variance and heritability values were higher in later harvests. The additive genetic correlations were moderate to low in early measurements and peaked at intermediates, with fairly stable values across the environmental gradient, except for the initial harvest data collection. This led to the conclusion that complex genotype-by-environment interaction (G×E) arises from spatial and temporal dimensions in the early season, with lower re-ranking trends thereafter. In general, modeling the temporal dimension with a second-order orthogonal polynomial in the reaction norm mixed model framework improved the accuracy of genomic estimated breeding value prediction for nutritive quality traits, but no gain in prediction accuracy was detected for dry matter yield. This study leverages the flexibility and usefulness of gRRM models for perennial ryegrass research and breeding and can be readily extended to other multi-harvest crops.

Introduction It is well-known that people with dementia living in residential care facilities spend most of their time not engaged in meaningful activities. Being involved in activities can improve their quality of life. Animal-assisted therapy is an activity that can evoke engagement and joy. Another way to create engagement and promote communication between people with dementia and caregivers is to use photos. Methods In this observational study, the researchers combined animal-assisted therapy and photobooks in order to explore whether photos of people with dementia who were engaged in animal-assisted therapy could be used as a tool for communication in dementia care. Ten persons (4 men and 6 women; aged 72–92) with dementia were video recorded during photo-elicited conversations with a dog handler/assistant nurse. The recordings were conducted in two residential care facilities in Sweden during 2017–2018. Each participant was video recorded 2–4 times. The recordings were analysed using The Observed Emotion Rating Scale and The Observational Measurement of Engagement-OME Modified. In addition, the video recordings were interpreted from a hermeneutic perspective. Findings The findings showed that the most frequently observed effects were pleasure and general alertness, and the participants were observed to be attentive most of the time. The most common attitude during the conversations was ‘somewhat positive’. The hermeneutic interpretations were grouped into four themes: Conveys a structure for the conversation with an inherent beginning and ending; An opportunity to recognise oneself and recall a sense of belonging; Awakens emotions and creates fellowship and Entails confirmation and revitalises their identity. Conclusion Photobooks can be used by caregivers as a tool for meaningful and joyful communication with people with dementia, even those with severe dementia.

Anmeldelse af Dan Ringgaard: Chaplins pind – Et essay om litteratur og kreativitet, Aarhus Universitetsforlag, 2020, 225 sider, 199,95 kr.

Carbon 179, 20-27 (2021). doi:10.1016/j.carbon.2021.03.061, Carbon fibres show great potential as multifunctional negative electrode for novel structural battery composites – a rechargeable electrochemical cell with structural function. The electrochemical performance of carbon materials can be enhanced with nitrogen heteroatoms, which conveniently are inherent in polyacrylonitrile (PAN)-based carbon fibres. However, it is not fully understood how the electrochemical performance is governed by microstructure and composition of the carbon fibres, particularly the distribution and chemical states of nitrogen heteroatoms. Here we reveal the atom-by-atom three-dimensional spatial distribution and the chemical states of nitrogen in three PAN-carbon fibre types (M60J, T800 and IMS65), using atom probe tomography (APT) and synchrotron hard X-ray photoelectron spectroscopy (HAXPES), and correlate the results to electrochemical performance. The findings pave the way for future tailoring of carbon fibre microstructure for multifunctional applications., Published by Elsevier Science, Amsterdam [u.a.]