1. Ultrastructural and immunohistopathological evaluation of intravesical ureters via electron and light microscopy in children with vesicoureteral reflux
- Author
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Sait Polat, Volkan Izol, Nihat Satar, Yurdun Kuyucu, Mutlu Deger, Arbil Acikalin, Ibrahim Atilla Aridogan, Çukurova Üniversitesi, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümü, Üroloji Anabilim Dalı, Çukurova Üniversitesi, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümü, Tıbbi Patoloji Anabilim Dalı, Çukurova Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Histoloji ve Embriyoloji Anabilim Dalı, İzol, Volkan, Avcı Açıkalın, Arbil, Kuyucu, Yurdun, Değer, Mutlu, Arıdoğan, İbrahim Atilla, Polat, Sait, and Satar, Nihat
- Subjects
Male ,medicine.medical_specialty ,Pathology ,Adolescent ,Urology ,Urinary Bladder ,CD34 ,Autopsy ,Electron ,Vesicoureteral reflux ,chemistry.chemical_compound ,Ureter ,Microscopy, Electron, Transmission ,medicine ,Humans ,Child ,High-power field ,Vesico-Ureteral Reflux ,Microscopy ,business.industry ,Reflux ,Infant ,medicine.disease ,Immunohistochemistry ,Vascular endothelial growth factor ,medicine.anatomical_structure ,chemistry ,Child, Preschool ,Female ,business - Abstract
PubMedID: 24126283 Purpose We used immunohistochemical methods and transmission electron microscopy to investigate the cytokine profiles and ultrastructural changes in the ureterovesical junction of children with primary vesicoureteral reflux. Materials and Methods A total of 39 distal intravesical ureters were obtained from 23 children who underwent ureteroneocystostomy for primary vesicoureteral reflux. Ureteral wall smooth muscle organization and transforming growth factor-ß1, vascular endothelial growth factor and CD34 were evaluated immunohistochemically and compared to controls, which consisted of 10 age matched autopsy specimens. Ultrastructural evaluations and morphological descriptions were performed semiquantitatively and compared to the published data. Results Of the patients 6 (26%) were male and 17 (74%) were female, and mean ± SD age was 73.2 ± 34.3 months (range 12 to 168). There was no correlation between reflux grade and age (p = 0.39). Smooth muscle disorganization score differed significantly between patients with intravesical ureters and controls (p = 0.01). Transforming growth factor-ß1 levels were significantly higher (p = 0.001) and vascular endothelial growth factor levels and microvessel densities were significantly lower in the patients with reflux compared to controls (both p
- Published
- 2013