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Ultrastructural and immunohistopathological evaluation of intravesical ureters via electron and light microscopy in children with vesicoureteral reflux
- Source :
- The Journal of urology. 191(4)
- Publication Year :
- 2013
-
Abstract
- PubMedID: 24126283 Purpose We used immunohistochemical methods and transmission electron microscopy to investigate the cytokine profiles and ultrastructural changes in the ureterovesical junction of children with primary vesicoureteral reflux. Materials and Methods A total of 39 distal intravesical ureters were obtained from 23 children who underwent ureteroneocystostomy for primary vesicoureteral reflux. Ureteral wall smooth muscle organization and transforming growth factor-ß1, vascular endothelial growth factor and CD34 were evaluated immunohistochemically and compared to controls, which consisted of 10 age matched autopsy specimens. Ultrastructural evaluations and morphological descriptions were performed semiquantitatively and compared to the published data. Results Of the patients 6 (26%) were male and 17 (74%) were female, and mean ± SD age was 73.2 ± 34.3 months (range 12 to 168). There was no correlation between reflux grade and age (p = 0.39). Smooth muscle disorganization score differed significantly between patients with intravesical ureters and controls (p = 0.01). Transforming growth factor-ß1 levels were significantly higher (p = 0.001) and vascular endothelial growth factor levels and microvessel densities were significantly lower in the patients with reflux compared to controls (both p
- Subjects :
- Male
medicine.medical_specialty
Pathology
Adolescent
Urology
Urinary Bladder
CD34
Autopsy
Electron
Vesicoureteral reflux
chemistry.chemical_compound
Ureter
Microscopy, Electron, Transmission
medicine
Humans
Child
High-power field
Vesico-Ureteral Reflux
Microscopy
business.industry
Reflux
Infant
medicine.disease
Immunohistochemistry
Vascular endothelial growth factor
medicine.anatomical_structure
chemistry
Child, Preschool
Female
business
Subjects
Details
- ISSN :
- 15273792
- Volume :
- 191
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- The Journal of urology
- Accession number :
- edsair.doi.dedup.....3ae91268637f977162a9c15df5a371e0