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The aim of this prospective study was to assess whether the Sequential Organ Failure Assessment (SOFA) score could be indicative of outcome (survival to discharge) in dogs with parvoviral enteritis.In 35 naturally infected dogs, the SOFA score and clinical score were calculated and the presence of systemic inflammatory response syndrome was verified on admission and during the first four days of hospitalization.26 dogs survived, and out of the 9 non-survivors, 6 dogs had positive blood cultures. Mean SOFA scores and clinical scores between survivors and non-survivors and between septic and non-septic dogs on admission and on each hospitalization day were significantly different. Trends in SOFA score indicated that in non-survivors and septic dogs there was an increase in SOFA score during the first four days of hospitalization and a decrease occurred in survivors and non-septic dogs. The area under the curve (ROC curve analysis) for SOFA score predicting the outcome was 0.797 and predicting sepsis was 0.834. The best cut-off point of SOFA score for predicting the final outcome was 3.5 and the best cut-off of SOFA score for predicting sepsis was also 3.5.Either single values or trends in SOFA score can assist in suspecting sepsis and reaching prognosis in parvoviral enteritis.

The objective of the current study was to investigate the echotextural and hemodynamic changes of ewes entering the dry period with or without subclinical mastitis. B-mode and color Doppler ultrasonography were applied to 12 Chios ewes (6 with healthy udders (group A) and 6 with subclinical mastitis (group B)) before the dry period, during the dry period (the involution phase, steady state, and transition phase), and postpartum. The color Doppler of the mammary arteries was used to evaluate them according to the pulsatility index (PI), resistive index (RI), end-diastolic velocity (EDV), time-averaged maximum velocity (TAMV), blood flow volume (BFV), and artery diameter (D). Udder parenchyma images, analyzed by Echovet v2.0, were used to evaluate the mean value (MV), standard deviation (SD), gradient mean value (GMV), gradient variance (GV), contrast (Con), entropy (Ent), gray value distribution (GVD), run length distribution (RunLD), and long run emphasis (LRunEm). In the involution phase, the PI was higher in group B compared to group A (p ≤ 0.05). The PI and RI were higher postpartum, whereas the EDV, TAVM, and D were higher in the transition phase (p ≤ 0.05). Neither the period nor the ewe group affected the MV, SD, GMV, GV, Con, and GVD values (p ≤ 0.05). In the steady state, the LRunEm was higher in group B, but postpartum, it was higher in group A (p ≤ 0.05). In conclusion, B-mode and Doppler can reveal differences (i) between healthy ewes and ewes with subclinical mastitis and (ii) among the different periods studied. Further research is needed on the blood flow and echotexture indices of the udders of ewes with unilateral subclinical mastitis.

Managing a milk zone in the dairy industry is demanding. Data necessary for efficient management are difficult to acquire because they usually must be collected in organized and standardized ways. On the other hand, software practices constantly provide new tools that can go beyond simple record-keeping practices and add value to the data. In this work, FarmDain is a novel web-based application for sheep and goat management. It aims to improve milk production and processing by digitizing the value chain in data acquisition, processing and visualization between dairy production businesses and their milk suppliers. FarmDain uses state-of-the-art software technologies to model the data collection process and provides a straightforward user interface to facilitate data processing and visualization. Using the app in a case study carried out for 12 months in a dairy sheep farm resulted in lower feeding cost per milked ewe by 5.5% when ewes were allocated into high and low milk production groups compared to the scenario of remaining in one single group. Furthermore, based on reports provided by the app, culling and genetic selection decisions were made to improve the overall farm performance. Similar practices were applied in all farms optimizing their productivity, which led to increased profitability for farms and the Dairy Factory.

The present study aimed to investigate the impact of age, season and ejaculation on ram testicular blood flow and echotexture. The survey was conducted biweekly on 7 Chios rams for one year, including breeding and non-breeding periods. The rams were divided into 2 age groups: 3 rams 2-6 years old (mature) and 4 rams 9-13 years old (old). Hemodynamic indices [Pulsatility index (PI), Resistive index (RI), End-diastolic velocity (EDV), testicular artery Diameter (D), Time-averaged maximum velocity (TAVM), Blood flow volume (BFV)] and echotexture parameters [Mean value (MV), Contrast (Con), Gray value distribution (GVD), Run length distribution (RunLD), Long run emphasis (LRunEm), Entropy (Ent), Correlation (Cor), Standard deviation (StD), Gray variance (GV) and Gradient mean value (GMV)] were evaluated in each testis before and after ejaculation. Ejaculation did not affect testes blood flow or echotexture (p0.05). PI and RI were higher in the breeding period compared to the non-breeding period, for both testes (p⟨0.001). Left testis GV and Cor before ejaculation were lower (p=0.01) and higher (p=0.03), respectively, in the breeding compared to the non-breeding period. Left testis D (p=0.005) and BFV (p⟨0.001) were higher in old compared to mature rams after ejaculation. Right testis Con (p=0.03) and Cor (p=0.05) before ejaculation were higher in old rams, whereas right testis Ent after ejaculation was higher in mature rams (p=0.05). In conclusion, testicular blood flow and echotexture are affected by season and ram age, but not by ejaculation.

Filarioids of the genus Cercopithifilaria (Spirurida, Onchocercidae) are parasites of wild and domestic animals in tropical and subtropical regions being transmitted by ixodid ticks. Though this filarioid species have been studied in canine and tick populations in Europe, data on their species diversity and geographical distribution in Greece is scant. Thus, the aims of this study were to investigate the presence of Cercopithifilaria spp. in dogs and ticks across Greece and to assess the possible risk factors. A total of 500 skin biopsies were collected from dogs, while 508 ticks were collected from 180 infested animals and examined. Sediments from skin biopsies were microscopically screened for detection of dermal microfilaria (mfs). Skin samples (n = 115) and tick specimens (n = 153) were molecularly subjected by PCR. Overall, 70 samples (14%) scored positive for mfs. Specifically, 68 samples (13.6%) were positive for Cercopithifilaria bainae and two (0.4%) were co-infected with C. bainae and Cercopithifilaria sp. II. Molecular analyses revealed that all sequences obtained belong to C. bainae. Haplotype I was the most frequent (92.6%), followed by haplotype XVIII (3%) and haplotypes II and IX (1.5%). Three new haplotypes of C. bainae, named XIX, XX, and XXI, were also identified. Among the risk factors examined, habitat, dog use, body weight, tick infestation history, and the use of acaricides were associated with the presence of C. bainae. The estimated prevalence of Cercopithifilaria spp. demonstrates that these filarioids are common in dogs and ticks in Greece. Finally, the identification of 7 haplotypes for C. bainae confirms their genetic variability.

Contrast-enhanced voiding urosonography (CE-VUS) has been generally considered as a promising tool to diagnose vesicoureteral reflux and abnormalities in lower urinary tract in human patients, especially in children. The purpose of this prospective study is to evaluate the quality of images of the urinary bladder and urethra obtained by CE-VUS using a second-generation ultrasound contrast agent (SonoVue®) in healthy dogs and to investigate the safety profile of SonoVue® after intravesical administration. Eighty-four CE-VUS examinations with SonoVue® were successfully performed in both unsedated (39/84) and sedated (45/84) dogs. Contrast-enhanced voiding urosonography examination of urinary bladder was technically successful in all (84/84) dogs. The image quality was not considered adequate in five (5/84) dogs including three dogs in whom layering of contrast media during filling phase was observed and two dogs with premature destruction of microbubbles. In these five dogs, the problem was readily recognized and corrected such that the procedure was still successfully undertaken. The assessment of the urethra during spontaneous micturition was successfully performed in all (84/84) dogs in whom voiding was elicited during the examination. No side effects were observed after intravesical application of SonoVue®. This study demonstrates that CE-VUS is a feasible and valuable technique to evaluate low urinary tract morphology and function in dogs. Based on our review of the literature, there are no published reports about the use of this method in dogs.

Dairy sheep farming is a significant agricultural sector in Mediterranean countries, providing income and employment opportunities in less-favoured areas (LFAs). The economic performance of dairy sheep farms is of significant interest to LFAs. However, relevant literature is scarce. The objectives here were to evaluate the economic performance of dairy sheep farms in LFAs of Greece and perform a comparative analysis based on flock size and farming system. In total, 19 and 26 dairy sheep farms for two production periods were used. Farm technical (flock size, production, grazing and nutritional management) and economic (income and variable costs) data were collected. The economic performance of farms was estimated using Happy Goats, a decision support tool for small ruminant farming. Estimated economic parameters were analysed by flock size (≤150 ewes vs. >150 ewes) and farming system (intensive/semi-intensive vs. semi-extensive). Results showed that 37% and 31% of farms were operating with losses in each production period, respectively. Based on nutritional management, ewes produced about 50 kg less milk per milking period. Smaller and semi-extensive farms had significantly (p < 0.05) lower incomes and variable costs. A significantly (p < 0.05) lower average gross margin was reported for smaller compared to larger farms. Results suggest a better economic perspective for larger flock sizes.

The level of technical efficiency (TE) of farms is a complex issue largely connected to the efficient use of available resources, which in turn determines their economic performance. Data envelopment analysis (DEA) is an established method to estimate TE; however, it is subject to drawbacks which sometimes reduce its ability to account differences in production scales. Indeed, the conventional DEA model allows total flexibility in the weights that a decision-making unit attaches to inputs and outputs, while in some cases, zero weights are attached to variables that are totally omitted in the efficiency analysis. Restricting efficiency weights in the DEA model, without of course eliminating the total weight flexibility assumption, guarantees zero weights, and prevents large differences in weights. In this study, an assurance region (AR) weight restricted model is applied on 66 mussel farms in order to calculate more comprehensive efficiency estimates and to obtain a meaningful and consistent picture of the efficient farm structure in economic terms, which could be potentially used for managerial suggestions, identification of best practices and innovations, and effective decision-making tool concerning mussel farm aquaculture. The cost share of the main production factors is used for imposing weight restrictions on the conventional DEA model, and a comparative descriptive technical and economic analysis of the efficient farms of the restricted and unrestricted DEA models is implemented. The results indicate that the structure of the efficient farm under the restricted DEA model is substantially diversified, formulating a new pattern of production system that achieves a higher economic performance.

This paper examines the financial viability and potential socioeconomic effects of introducing and operating an automated, remote-controlled management system for mussel farms which uses probes of temperature, dissolved oxygen, and conductivity associated with prediction software to demonstrate the potential need for mussel movement between marine areas. This system provides an early warning to farmers regarding the presence of toxins in aquatic ecosystems, thus contributing to saving mussel production and avoidikng significant economic losses. The analysis combines two established methodological tools in agricultural economics (linear programming and cost-benefit analysis) and provides estimates of the Net Present Value of the investment under two scenarios—one reflecting the existing situation and one a possible future situation where the mussel production system is expanded. The results of the analysis reveal the mid- and long-term effects of using the automated system, both of which demonstrate that the system is economically viable even if it contributes to saving mussel production from toxicity occurrence for only one year during its period of operation. The annual gross margin in the first scenario was €386,069 but almost tripled in the second scenario (€1,154,649). In addition, the future development and expansion of the mussel sector will likely be based on larger farms with an entrepreneurial and exporting orientation where risk mitigation systems, such as the one appraised in this paper, can play an important role.

Global warming may accelerate growth and distribution of pathogens influencing aquatic organisms' diseases and human health. Despite the extensive research, the biology, cellular development and life cycle and of Marteilia sp. parasites as well as the influence of parasitic infection on the hosts are not fully understood. The aim of this study was to investigate the effect of Marteilia sp. prevalence and infection intensity on mussels’ growth rate and morphometric characteristics under natural conditions in Thermaikos Gulf, a major bivalve production area in Greece, during a five-month growth period. The length, width, height and weight of the infected mussels were significantly lower compared to non-infected and the decrease was proportional to the intensity of mussel infection by the parasite. Moreover, the estimation of allometric relations between length, height, width and weight revealed significantly lower growth of mussel wet weight in relation to shell length for infected mussels compared to healthy ones. The negative effect of marteiliosis on the shell length growth rate of infected mussels was also confirmed by von Bertalanffy equations.

Filarioids of the genus Cercopithifilaria (Spirurida, Onchocercidae) are parasites of wild and domestic animals in tropical and subtropical regions being transmitted by ixodid ticks. Though this filarioid species have been studied in canine and tick populations in Europe, data on their species diversity and geographical distribution in Greece is scant. Thus, the aims of this study were to investigate the presence of Cercopithifilaria spp. in dogs and ticks across Greece and to assess the possible risk factors. A total of 500 skin biopsies were collected from dogs, while 508 ticks were collected from 180 infested animals and examined. Sediments from skin biopsies were microscopically screened for detection of dermal microfilaria (mfs). Skin samples (n = 115) and tick specimens (n = 153) were molecularly subjected by PCR. Overall, 70 samples (14%) scored positive for mfs. Specifically, 68 samples (13.6%) were positive for Cercopithifilaria bainae and two (0.4%) were co-infected with C. bainae and Cercopithifilaria sp. II. Molecular analyses revealed that all sequences obtained belong to C. bainae. Haplotype I was the most frequent (92.6%), followed by haplotype XVIII (3%) and haplotypes II and IX (1.5%). Three new haplotypes of C. bainae, named XIX, XX, and XXI, were also identified. Among the risk factors examined, habitat, dog use, body weight, tick infestation history, and the use of acaricides were associated with the presence of C. bainae. The estimated prevalence of Cercopithifilaria spp. demonstrates that these filarioids are common in dogs and ticks in Greece. Finally, the identification of 7 haplotypes for C. bainae confirms their genetic variability.

Background Diabetes is regarded as an epidemiological threat for the twenty-first century. Phytochemicals with known pharmaceutical properties have gained interest in the field of alleviating secondary complications of diseases. Such a substance is crocin, a basic constituent of saffron (Crocus sativus). The present study aimed at examining the beneficial effects of per os crocin administration on the antioxidant status, blood biochemical profile, hepatic gene expression and plasminogen activator inhibitor-1 activity (PAI-1) in the liver, kidney and plasma (an important marker of pre-diabetic status and major factor of thrombosis in diabetes) of healthy rats, as well as of rats with nicotinamide-streptozotocin-induced diabetes. Results Diabetes disrupted the oxidation-antioxidation balance, while crocin improved the antioxidant state in the liver by significantly affecting SOD1 gene expression and/or by restoring SOD and total antioxidant capacity (TAC) levels. In the kidney, crocin improved hydrogen peroxide decomposing activity and TAC. In blood, hepatic transaminases ALT and AST decreased significantly, while there was a trend of decrease regarding blood urea nitrogen (BUN) levels. The expression of PAI-1 gene was affected in the liver by the dose of 50 mg kg−1. Conclusions Crocin treatment contributed in restoring some parameters after diabetes induction, primarily by affecting significantly hepatic transaminases ALT and AST, SOD1 and PAI-1 gene expression and nephric H2O2 decomposing activity. In conclusion, crocin did contribute to the alleviation of some complications of diabetes.

The present study aimed to provide new and objective information regarding the effect of season and age on the characteristics of Chios ram semen. The semen collection was performed biweekly throughout a year, including both breeding and non-breeding periods. Seven active Chios rams were divided in two age groups: three rams of 2–6 years old (mature) and four rams of 9–13 years old (old). After collection, semen volume and sperm concentration were measured. One hundred sixty-eight (168) semen samples were collected, extended and evaluated. All samples were tested for motility and kinetics (Computer Assisted Sperm Analyzer), viability-membrane integrity (Eosin-Nigrosin), morphology (SpermBlue®), chromatin integrity (Acridine Orange Test) and sperm membrane biochemical functionality (Hypo-osmotic swelling test, HOST). Motility did not differ significantly between periods (p > 0.05) or between age groups (p > 0.05). The percentage of hyperactivated spermatozoa and head abnormalities was higher in the non-breeding compared to the breeding period (p = 0.011 and p = 0.001, respectively). Tail abnormalities were higher in the breeding (p = 0.022) and HOST + spermatozoa were lower in the non-breeding period (p

Our objective was to classify dairy goat farms into representative typologies. We used a random sample of 103 goat farms from 8 regions in mainland Greece and the islands. Farmers were surveyed using a designated questionnaire for in depth interviews on their farms. A general description of production methods and farm characteristics was recorded. The farms were classified using a multivariable statistical approach including principal component analysis and cluster analysis. The differences between clusters were assessed using one-way ANOVA and χ 2 test and the profitability of farms was estimated. The intensity of production system was mostly classified based on goat herd size and age of kids at weaning. Reproductive management was classified mostly based on age at first mating and does to bucks ratio. Herd turnover rate and genetic improvement strategy was mostly classified by bucks’ replacement rate and doelings to does ratio. Cultivated land per livestock unit, irrigated land used, years of farmer's experience, and facilities and equipment score were significant farming descriptors. We identified four clusters that shared common characteristics and major differences. Cluster 1 (26 farms, 25.2%) included large, semi-intensive, high producing and investing farms. Cluster 2 (50 farms, 48.5%) included semi-extensive, low-input, traditional farms. Cluster 3 (12 farms, 11.7%) included medium-sized, semi-intensive, low replacement rate farms. Cluster 4 (15 farms, 14.6%) included semi-extensive, low-input, traditional farms, producing heavy weight kids carcasses. Regarding profitability, Cluster 1 was significantly more profitable than clusters 2 and 4, whereas the more profitable clusters also had more variation in profit suggesting they are riskier. Cluster 4 could benefit from further developing meat production, processing and packaging to become an independent cluster. This new cluster would be focused on both milk and meat whilst the other three clusters focus on milk.

In this study, the non-parametric data envelopment analysis was applied in a sample of 66 mussel aquaculture farms for the estimation of the level of technical efficiency. The differences in this estimated level of efficiency were investigated through the application of a Tobit regression model and a technical and economic descriptive analysis provided an indicative picture of the structure and the economic performance of the efficient farms. The results indicated significant inefficiencies in the utilization of the existing production technology. The estimated mean technical efficiency was 0.761, indicating that the mussel farms could increase their production by 23% given the level of inputs. Moreover, the results of the Tobit regression model showed that socio-demographic variables, such as farmer’s age, experience in aquaculture, vocational training, and level of education, can partly explain the efficiency differentials. The technical and economic analysis showed that the efficient mussel farms compared to the inefficient farms are larger in size, use less labor per hectare of sea area, and achieve higher net profit.

Pseudorabies is an acute, frequently fatal disease that mainly affects pigs and incidentally other animals. Although pseudorabies virus (PRV) has been eradicated from many European countries, it is still endemic in East and Southeast parts of Europe. Greece belongs to the countries where the disease is enzootic. In this study, we investigated the presence of PRV in Greek farms. For that reason, 42 pig farms were selected from the entire Greek territory. Blood samples from different age groups had been collected from each farm and were tested by ELISA for the presence of antibodies against wild strains of PRV. The results of our study showed that 28.6% of the selected farms were positive for the presence of antibodies against wild-type strains of PRV and that factors such as the non-implementation of biosafety measures and the high density of pig farms in an area may affect the probability of a farm to become PRV positive. This study provided some useful information with regard to the presence of PRV in the domestic pigs in Greece. This information may assist in designing and implementing measures in order to control and eradicate the disease from the domestic pigs in Greece.

The multifunctional farming sector in Cyprus poses threats on the island’s water resources, but highly contributes to the protection of the cultural identity and to the provision of incomes and employment in its rural areas. These services are externalities, as farmers are not remunerated in markets for the environmental and cultural services they provide, nor for the fact that they maintain vivid rural areas. This paper presents an application of the Choice Experiment method, in order to evaluate these non-traded outputs of Cypriot agriculture. The results of the empirical analysis demonstrate that the Cypriot public is in favor of a less intensive pattern of agriculture. Furthermore, Cypriots are willing to pay in order to mitigate adverse environmental effects of agriculture, to improve cultural heritage and to safeguard the continuation of farming trade on the island. The estimated benefits often exceed income losses from changes in the cropping pattern towards extensification, which verifies that EU rural development policies are regarded as beneficial by the public.

The aim of the study was to determine whether the presence of astaxanthin (ASX) protects boar spermatozoa against damage related to cryopreservation. Pooled ejaculates extended in Beltsville Thawing Solution (BTS) were used. Three experiments were conducted: 1) sperm samples were pre-incubated overnight (17 °C) with ASX (0, 0.5, 5, 15 μM) prior to freezing and then frozen using cooling and thawing extenders supplemented with ASX (0, 0.5, 5, 15 μM); 2) sperm samples were treated with ASX (0, 0.5, 5, 15 μM) only during overnight pre-incubation (17 °C) prior to cryopreservation; and 3) a thawing extender was supplemented with ASX (0, 0.5, 5, 15 μM). The groups were as follows: control (C; no treatment), ASX 1 (0.5 μM), ASX 2 (5 μM) and ASX 3 (15 μM). Total (TM) and progressive (PM) motility was analyzed using CASA, while sperm viability, reactive oxygen species generation, lipid peroxidation and apoptoticlike changes were analyzed using flow cytometry. Sperm variables were evaluated prior to freezing as well as 30 and 150 min after thawing. In Experiment 1, the values of TM and sperm viability post-thaw were less in the ASX 3 than C group. In Experiment 2, there was no effect of ASX on any of the sperm variables evaluated, while in Experiment 3, apoptotic-like changes were less in the ASX 1 than C group. In conclusion, there was a subtle beneficial effect on cryopreserved boar spermatozoa after addition of ASX to thawing media.

European sheep and goat farming faces diverse challenges at global or local scales and constitutes an important sector for many countries, playing important sociocultural, economic and environmental roles. A closer examination of the overall sustainability of the sector is necessary to assess the performance of different farm types in various geographical settings. This comparative study evaluates the use of a common sustainability assessment (SA) tool for the major European countries in the sheep and goat sector. In particular, the study reports the results of a SA using the Public Goods (PG) Tool, adapted within the Innovation for Sustainable Sheep and Goat Production in Europe (iSAGE) Horizon 2020 project, which includes questions accounting for 13 dimensions of a sheep and goat farm sustainability. In total, 206 farmers from Greece, Italy, Spain, Finland, United Kingdom, France and Turkey were interviewed, all of which were typical of specific types of a pan-European sheep and goat farm typologies elaborated within iSAGE. The study resulted in composite indicators of performance in each dimension for each country. Finland, Italy and the United Kingdom performed better than other countries, while Turkey and Greece performed below average in most categories. The results highlight challenges for each country but also at the European level, the latter mainly relating to generational renewal and an unwillingness to invest in the adoption of a more sustainable approach with long-term results.

Objective: Limited data exist regarding the correlation between adipose-derived stromal vascular fraction (SVF) and wound healing. The aim of this study was to investigate the direct effect of intradermally injected SVF on full-thickness cutaneous wounds in a murine model. Method: Wistar rats were divided into three groups (A, B and C) according to their day of euthanasia (day 7, 16 and 21). Inguinal fat pad was excised and SVF enzymatically extracted. Full-thickness cutaneous wounds were created on each side of the dorsum; SVF injected intradermally at one side while the contralateral wound served as control receiving normal saline. Postoperatively, evaluation of wound healing was performed by planimetry (percentages of wound contraction, epithelialisation and total wound healing) on days 0, 3, 5, 7, 10, 13, 16 and 21, and histology and immunochemistry (cellular infiltration score, collagen production score, neoangiogenesis and epithelial thickness) on days 7, 16 and 21. Results: Despite the high rate of wound contraction, it was significantly lower in the SVF-treated wounds on day 21 (p=0.037). On days 13, 16 and 21, the percentages of epithelialisation were higher in the SVF-treated wounds compared with control wounds (p=0.026, p=0.048 and p=0.05, respectively). Histologically, the number of new vessels was significantly higher in the SVF-treated wounds compared with controls on days seven (p=0.028) and 16 (p=0.027). This was also confirmed by immunohistochemistry. No significant differences were found between treated and control wounds regarding cellular infiltration score, collagen production score and epithelial thickness. Conclusion: Data indicate that intradermally injected SVF increases angiogenesis and enhances epithelialisation in full-thickness cutaneous wounds in rats.

This study investigated correlations among ram semen quality parameters, testicular vascularity and echotexture. Measurements were performed biweekly for a year, before (BE) and after ejaculation (AE), on seven Chios breed rams [three rams (group A) and four rams (group B) of 2-6 and 9-13 years old, respectively], considering breeding (1) and non-breeding period (2). Hemodynamics [Pulsatility Index (PI), Resistive Index (RI)] were evaluated in each testis. Images of testicular parenchyma were analysed to evaluate echogenicity [Mean Value (MV), Contrast (Con), Gray Value Distribution (GVD), Run Length Distribution (RunLD), Long Run Emphasis (LRunEm)]. Semen volume (V), concentration (C), kinetics (CASA), morphology (Sperm Blue), viability (Eosin-Nigrosin) and DNA fragmentation (Acridine Orange) were assessed. In group A/2, semen V was correlated to left testis PI and right testis RI, BE (r = 0.4, p = 0.013/r = 0.6, p

In this study the technical efficiency level of dairy farms in Greece is estimated by imposing weight restrictions on the conventional data envelopment analysis (DEA) model. The assurance region approach is applied on farm record and accounting data from 165 dairy farms. The numerical values of the weight bounds are determined by placing limits on the estimated output elasticities and on the factors' cost share of the total production cost. The imposition of bounds on the DEA weights provides more persuasive efficiency scores and alters the structure of the efficient farms, verifying the importance of placing weight restrictions when dealing with real-life applications.

This field study assessed the efficacy of a probiotic based on viable spores of Bacillus subtilis C-3102 (Calsporin; Calpis Co. Ltd., Japan) on the health status and productivity of sows and their litters through 2 full, sequential reproductive cycles from service of the first cycle to weaning of the second cycle. Fifty-six sows were allocated to 2 experimental groups, an untreated control (T1) group and a probiotic-treated (T2) group that received the same basal feed as the T1 group plus the probiotic at an approximate allowance of 30 g/t of feed (3 × 10(5) cfu/g). The offspring of T1 and T2 sows were offered basal and T2 creep feed (3 × 10(5) cfu/g), respectively. Health and zootechnical parameters of sows and piglets were recorded. Feeding the probiotic to sows and piglets resulted in significant benefits, observed in both cycles: 1) improved sow body condition during pregnancy (P < 0.05), 2) increased sow feed consumption, 3) reduced sow weight loss during lactation (P < 0.05), 4) reduced sow weaning-estrus interval (P < 0.05), and 5) higher BW of piglets at weaning (P < 0.05). Additionally, a significant (P < 0.05) improvement in piglet birth weight and in the number of piglets weaned was observed in the second cycle of T2 sows, while a significant improvement of mean daily gain of piglets from birth to weaning was observed in the first cycle of T2 sows. Microbiological examination of fecal samples showed that probiotic treatment significantly reduced both Escherichia coli and Clostridium spp. in piglet feces, particularly during the second cycle. The data suggested that continuous feed supplementation with the probiotic is beneficial for both sows and piglets, since zootechnical benefits were observed in both cycles.

Dairy farming in Greece constitutes a particular case of livestock farming, because of its highly entrepreneurial nature. The modern Greek dairy cattle farming is predominantly based in Northern Greece, where more than 80% of Greek cow milk is produced. Since the 2000's the dairy cattle sector is characterized by a rapid increase in the mean size of farms, which permits them to undertake investments in machinery and buildings and to have access to improved genetic material for the achievement of higher productivity. Nevertheless, this development renders dairy farms vulnerable to volatile economic conditions, as they are heavily dependent on capital endowments. The main purpose of this study is to examine the consequences of the economic crisis on the dairy cattle sector and the strategies undertaken by farmers to cope with it. The analysis is based on data from a questionnaire survey of farmers in Northern Greece. Strategies undertaken by dairy farmers in order to face the crisis and to ensure the survival of their businesses are recorded through a set of Likert-scale questions. Technical and economic data are also analyzed in order to examine the economic performance of farms, revealing that the sampled dairy farms are viable, with satisfactory economic results, with capital returns of 6.2% annually. Based on the Likert-scale data, dairy farmers adopt active strategies to face the crisis, by undertaking investments and augmenting their farm sizes, while they deem that the crisis does not have catastrophic results on the profitability of their farms. On the other hand, strategies which entail less expenses and/or diminished productivity are unpopular among dairy farmers. Finally, the vast majority of respondents claim to have seen negative consequences on their farms from the recent measures concerning the prolongation of the duration of fresh milk in retail markets: lower quality, increased vulnerability to international competition, shrinking dairy sector.

Cotton production in Uzbekistan is known as ‘white gold’ and constitutes a strategic centrepiece of the country's rural economy. However, the gradual ongoing process of market-oriented reform has had a severe impact on the cotton sector. The objective of this paper is to estimate the efficiency and total factor productivity growth of cotton farms in Uzbekistan. Data Envelopment Analysis (DEA) was applied to measure technical efficiency and the Malmquist Index was used to estimate and analyse productivity. The analysis was based on a sample of 26 cotton farms observed between 2007 and 2009. The results help to provide a set of policy recommendations regarding the sector's growth potential through the efficient utilization of resources, and indicate that there has been a moderate decline in productivity attributed to technical degeneration over the period studied.

Mussel farming in Greece constitutes an important economic activity for the areas where it is concentrated. This paper presents the results of a survey of mussel producers in Thermaikos Gulf, Northern Greece, where almost 90% of the total mussel production in the country takes place. Using data from a questionnaire survey of 66 mussel producers in the area, a typology is elaborated, by means of which three types of mussel farmers are discerned. “Professionals” are the ones continuing their family tradition, “Newcomers” are those who chose mussel farming as their main occupation and recently invested in the sector and “Aged amateurs” are the oldest and least educated group, members of which plan to pass their farm to younger family members. There is strong evidence that the sampled “Newcomers” achieve considerably higher financial results, however the sample size is not adequate to generalize for all results except for the farm income. When it comes to their environmental attitudes, mussel farmers comply to bans in cases where high toxin concentrates are detected, but they are not convinced about the effectiveness of controls. They discern very clearly between the environmental and regulatory measures necessary to boost the productivity of the sector, but they seem to proclaim by far the latter type of adjustments, including the resolution of location issues. Based on the profile of each cluster and the general results of the sample proposals are made concerning the implementation of the new Common Fisheries Policy.

Consulting agricultural services constitute a major key for implementing successfully sustainable development EU goals in rural areas. Today, in the context of the Rural Development Programme 2007-2013, there are initiatives for funding crop and livestock farms and entrepreneurship. Presently, there are bureaus offering consulting services and reliable information to t he Greek farmers regarding Information Technologies adoption and innovative e-tools, marketing and processing of agricultural products, certificated agro-products (ISO), modernization of agricultural holdings, etc. Farmers have the opportunity to participate in EU funded measures that support the development of new activities, such as innovative actions, marketing services, new information technologies software and web tools and promote production innovations through specific investments. This paper aims to study and evaluate the adoption of consulting from livestock farms and discuss future opportunities. The study is based on primary data collected from the prefecture of Rodopi, in Thrace/Northeastern Greece, through a farm management survey which was carried out during September and December 2011. A sample of 120 livestock farmers between the ages of 20 and 70 was surveyed for the empirical application of this study using a well-structured questionnaire. The survey took place in private consulting bureaus and locally at farms. The collected data were coded and statistically analyzed using SPSS. Data was classified with cluster analysis in order to identify and describe groups with common attitudes among farmers. Findings show that 54% of the farmers in the sample are interested in consulting and adopt information technology solutions, marketing and entrepreneurship issues, aiming to sustainability in the context of EU goals. The results also indicate that 63% of the farme rs have already participated in a program, while 37% have participated in the schemes promoting the establishment of young farmers. Moreover, 55% of them would prefer to be consulted by private consulting bureaus rather than government consulting divisions. Private consulting bureaus satisfy farmers who consider them as a significant support in their attempt to achieve the contemporary EU goals for sustainable development through the adoption of information technologies tools and innovative trade and marketing techniques. Farmers can be further classified in two groups that share similar either innovative or traditional aspects about Greek agriculture trends, in relation to their social characteristics.

Dairy farming, despite its indisputable contribution to the production of edible products consumed worldwide, more than often poses threats to the environment. The purpose of this study is to provide a typology of the dairy farms situated in Central Macedonia, Greece, based on their environmental management practices. The empirical analysis uses data from a survey of 123 dairy farms in the study area. The farms are categorized into alternative profiles, using a two-step cluster analysis; the clustering is based on the results of a Categorical Principal Component Analysis, by means of which the variables describing environmental management practices are grouped into dimensions. Then, the characteristics of farmers of each category are explored by estimating a Multinomial Logit Model. The results illustrate that when it comes to the environment, dairy farmers in Central Macedonia adopt diverse farming practices, some of which are detrimental, while others promote environmental protection and, at the same time, ensure acceptable incomes for dairy farmers.

Summary Oxidative stress is one of the major factors that contribute to poor semen quality and low rates of in vitro fertilization. Crocetin, a main constituent of saffron (Crocus sativus L.) possesses potent antioxidant activity, by scavenging reactive oxygen species (ROS) and/or enhancing the activity of intracellular antioxidant enzymes. The aim of this study was to investigate, for the first time, the effect of crocetin on the quality characteristics of bull spermatozoa and fertilization rate. For this reason, frozen/thawed bovine spermatozoa were incubated with crocetin (1, 2.5, and 5 μm), for 120 or 240 min, in the presence of a negative control, and evaluated in terms of motility, viability, acrosomal status, DNA fragmentation index, intracellular ROS, and lipid peroxidation. In order to evaluate the impact of crocetin on cleavage and blastocyst rate, the compound was added in the IVF medium at the previously identified optimal concentration (2.5 μm). The results indicate that incubation of spermatozoa with 2.5 μm of crocetin resulted in a statistically significant lower production of superoxide anion and hydrogen peroxide, lower lipid peroxidation, and in better maintenance of motility parameters, viability, and acrosomal integrity, with a very small number of cells with DNA fragmentation, compared to the other groups (p

In this study the level of technical efficiency of 58 Chios sheep farms is estimated with the implementation of Data Envelopment Analysis. This level of technical efficiency is used as a classification criterion and the main technical and economic indices of the farms are analyzed in order to investigate their contribution to the improvement of technical efficiency. Next, the structural and economic characteristics of the farms that utilize the existing technology efficiently are described and compared with the average farm. The results show the presence of substantial inefficiencies in the allocation of the existing resources among farms that rear Chios sheep in Greece, which indicates that there is considerable room for development. The estimated mean level of technical efficiency is 0.76, suggesting that the average farm could increase its value of production by 24%, given the level of inputs and the existing production technology. Furthermore, larger farms are associated with higher levels of efficiency. A technical and economic description indicates that the efficient farms, compared to the average Chios breed farm, use less land and labor, rear a smaller number of ewes, utilize less variable and fixed capital and achieve a higher income in order to produce the same level of output.

Non-parametric Data Envelopment Analysis (DEA) and parametric Stochastic Frontier Analysis (SFA) have become very popular in the analysis of productive efficiency. This study undertakes a comparison of the SFA and the constant returns to scale (CRS) and variable returns to scale (VRS) output-oriented DEA models, based on a sample of 240 farms in Bangladesh. However, the aim of this paper is not only to compare estimates of technical efficiency obtained from the two approaches, but also to examine the determinants of inefficiency. The detection and measurement of technical inefficiency and its determinants can be used as a basis of policy recommendations. The results from both the approaches indicated that there is a potential for increasing farm production through efficiency improvement and that farmers' age and educational level have a significant effect on efficiency.

One of the key members of the innate immune system in many vertebrate species is the family of Toll-like receptors (TLRs). These molecules, which initiate the innate immune response and mount an anti-microbial response in both vertebrates and invertebrates, have recently been identified in the chicken genome. The recent findings of chicken TLRs (cTLRs) expression in ovarian follicles during follicular growth and in response to lipopolysaccharide (LPS) infection are very important for reproductive physiology due to the transovarian transmission of Salmonella enteritidis (SE) in laying hens. The aim of this study was to investigate the expression of the ten cTLRs identified to date, in the chicken ovary in vivo and embryos during early embryonic development, to investigate whether sexual maturation affects their ovarian mRNA abundance and to investigate the transcriptional changes of TLRs in the chicken ovary in response to SE infection. RNA was extracted from embryos from day 3 to day 10 of embryonic development as well as from the ovaries of healthy prepubertal, sexually mature and aged birds, and from sexually mature and aged SE infected birds. RT-PCR analysis revealed that all TLRs apart from TLRs 1–1 and 2–2 were expressed in the ovary of sexually mature chickens, while all TLRs apart from TLR1–1 were expressed in the chicken embryos during embryonic development. Quantitative real-time PCR analysis revealed that the ovarian mRNA abundance of TLRs differ with respect to sexual maturation. SE infection resulted in a significant induction of TLR4, and 15 in the ovary of sexual mature birds, and in a significant induction of TLR15 in the ovary of aged birds, while a significant down-regulation was observed for TLR3 in the ovary of aged birds. These findings suggest that a TLR-mediated immune response mechanism exists in the chicken ovary.

Στην παραγωγή του Μeσογeιακού μυδιού Mytilus galloprovincialis, τα δύο συστήματα καλλιέργeιας που eφαρμόζονται στην Eλλάδα, eίναι οι πλωτές eγκαταστάσeις (‘long-lines’) και οι πασσαλωτές eγκαταστάσeις (‘on tables’). Στην παρούσα μeλέτη, eξeτάστηκe η eπίδραση του συστήματος καλλιέργeιας στο ποσοστό των μυδιών που παρασιτούνται από διάφορα παράσιτα. Για τον σκοπό αυτό, eπιλέχτηκαν τρeις πeριοχές στο Θeρμαϊκό κόλπο, μe παρόμοια χαρακτηριστικά και στις οποίeς eφαρμόζονται και τα δύο συστήματα καλλιέργeιας. Πραγματοποιήθηκαν δύο δeιγματοληψίeς, τον Ιούλιο και τον Οκτώβριο 2008. Από κάθe πeριοχή, συλλέχθηκαν 30 μύδια ανά σύστημα καλλιέργeιας και από κάθe μύδι λήφθηκαν δeίγματα ιστών για ιστολογική eξέταση από το σπλαχνικό σάκο, τα βράγχια και το μανδύα, προκeίμeνου να διαπιστωθeί η παρουσία παρασίτων. Ο αριθμός των μυδιών που παρασιτούνταν από τουλάχιστον ένα eίδος παρασίτου ήταν σημαντικά αυξημένος σe μύδια που καλλιeργούνταν μe το πασσαλωτό σύστημα. Πιο συγκeκριμένα, το ποσοστό των μυδιών που παρασιτούνταν από τα παράσιτα Urastoma cyprinae και Marteilia spp. ήταν σημαντικά αυξημένο στα μύδια που καλλιeργούνταν μe το πασσαλωτό σύστημα, eνώ δeν υπήρχe σχέση μeταξύ του συστήματος καλλιέργeιας και του ποσοστού παρασίτωσης από Eugymnanthea inquilina. Το ποσοστό παρασίτωσης από τα παράσιτα Myticola intestinalis, Steinhaus mytilovum και Proctoeces maculatus ήταν πολύ χαμηλό και στα δύο συστήματα καλλιέργeιας. Eπιπλέον, και στα δύο συστήματα καλλιέργeιας, το ποσοστό των μυδιών που παρασιτούνταν από τα παράσιτα E. inquilina και U. cyprinae αυξήθηκe σημαντικά τον Οκτώβριο, eνώ η αντίθeτη τάση παρατηρήθηκe για το παράσιτο Marteilia spp.

Reactive oxygen species (ROS) production above critical levels affects the genetic and functional integrity of spermatozoa by causing oxidative stress. Spermatozoa are susceptible to oxidative stress in terms of motility and fertilization capacity. Crocin (crocetin di-gentiobiose ester), a main constituent of Crocus Sativus L. (saffron), is known for its antioxidant activity by scavenging ROS, especially superoxide anion. The aim of the present study is to evaluate the effect of crocin on the quality characteristics of spermatozoa and fertilization rate. Frozen-thawed and washed spermatozoa from four different bulls were incubated with three different concentrations of crocin (0.5, 1, and 2 mM), for 120 and 240 minutes, in the presence of a negative control, and were evaluated in terms of motility, viability, acrosomal status, DNA fragmentation index, intracellular ROS, and lipid peroxidation. The most potent concentration of crocin (1 mM) was also added in the fertilization medium to test its impact on fertilization outcome. The results indicate that the incubation of spermatozoa with 1 mM of crocin resulted in a statistically significant lower production of ROS, lower lipid peroxidation and in better maintenance of motility, viability, and acrosomal integrity, with a very small number of fragmented cells, compared to the control and the other treated groups (P

This study proposes the valuation of wetland functions as an alternative to the conventional approach to wetland valuation, in order to derive indicators for decision-making in wetland management. It is illustrated that these functions can be valuated in terms of the goods and services they provide to society. Using a functional approach, the functions are identified and the goods and services they provide are explicitly allocated among them; then, the latter are valuated with the Contingent Valuation method. Statistical analysis of the data provides welfare measures that reflect the value of these functions. It is argued that the values of separate functions are more useful in policy-making than their aggregated value.

The use of alternative methods for teaching purposes is gradually increasing in higher education. In order to evaluate the usefulness of non-animal based practical classes in veterinary science, and to inform on possible benefits and limitations of these teaching tools, a questionnaire was designed and distributed to students. Although there was no complete agreement among the student responses, it was apparent that the majority of the students would like traditional training methods to be paired with alternative approaches, and expressed their desire to be exposed to as many humane modes of learning as possible. In addition, the students agreed that alternative teaching methods for training in veterinary science can reinforce existing knowledge that is required at the clinical stage, and that they can be effective supplements to traditional training methods. It was also concluded from the study that the use of new alternative approaches is very much appreciated by the students, whereas the validity and effectiveness of these methods are debatable, suggesting that further optimisation, proper application and evaluation of these alternative methods is required.

Background Grape extracts of the Greek species Vitis vinifera possess potent antioxidant properties in vitro. The freeze/thaw process and the preparation of semen during assisted reproductive techniques can adversely affect the functional integrity of spermatozoa. The objective was to assess the effect of three different concentrations (1 μg ml−1, 2 μg ml−1 and 5 μg ml−1) of a polyphenol-rich grape pomace extract on motility, viability, acrosomal and lipid peroxidation status of thawed bovine spermatozoa after 2 and 4 hrs of incubation. Results The results indicate that the percentage of “Rapid” spermatozoa remained significantly increased (p

Rooster infertility is a major concern in the poultry industry and protection of the male reproductive organs from pathogens is an essential aspect of reproductive physiology. During the last years, research on antimicrobial protection has elucidated the critical role of the antimicrobial peptides avian β-defensins (AvBDs) in the innate immunity in chickens. AvBDs have been reported to be expressed in the hen reproductive organs, providing protection against microbial pathogens including Salmonella Enteritidis (SE). However, mechanisms of antimicrobial protection of rooster reproductive organs and especially the testis, mediated by AvBDs are poorly understood. The aim of this study was to investigate the expression of the complete family of the 14 AvBD genes, in the rooster testis in vivo, to determine whether sexual maturation affects their testicular mRNA abundance and to investigate whether SE infection alters their expression. Expression analysis revealed that 9 members of the AvBD family, namely AvBD1, 2, 4, 5, 6, 9, 10, 12 and 14 were expressed in the testis. Quantitative real-time PCR analysis revealed that the mRNA abundance of three AvBDs was up regulated and of three AvBDs was down regulated with respect to sexual maturation. In addition, SE infection resulted in a significant induction of AvBD4, 10, 12 and 14 in the testis of sexually mature roosters. These findings provide strong evidence to suggest that an AvBD-mediated immune response mechanism exists in the rooster testis providing protection against bacterial pathogens including Salmonella species.

Avian β-defensins (AvβDs) constitute a family of antimicrobial peptides that are critical to innate immunity in chickens, providing protection against microbial pathogens including Salmonella Enteritidis (SE). As apart from the digestive tract another main route of SE colonization in birds is via infection of the oviduct and specifically of the vagina, the aim of this study was to investigate the expression of the complete family of AvβDs, in the chicken vagina in vivo, to determine whether sexual maturation affects their mRNA abundance and to investigate whether SE infection alters the vaginal AvβDs expression. Expression analysis revealed that 11 members of the AvβD family were expressed in the chicken vagina. Quantitative real-time PCR analysis revealed that the mRNA abundance of five AvβDs was up regulated and of one AvβD was down regulated with respect to sexual maturation. In addition SE infection resulted in a significant induction of AvβD5, 7, 10, 11, 12 and 14 in the vagina of sexually mature birds, and in a significant induction of AvβD5 and 11 in the vagina of aged birds. These findings provide strong evidence to suggest that an AvβD-mediated immune response mechanism exists in the chicken vagina providing protection against bacterial pathogens including Salmonella species.

This paper presents work-in-progress on a software project management simulator suitable for educating and training prospective software project managers. The work presents the design of a modular architecture for the simulator as well as a modeling language for representing possible management scenarios. It finally reports on issues one has to bear in mind when considering the development of such systems.

Rooster infertility is a major concern in the poultry industry and chicken male reproductive organs are the infectious tissues of various pathogenic microorganisms. Protection of the chicken male reproductive organs from pathogens is therefore an essential aspect of reproductive physiology. Recently Toll-like receptors (TLRs) have been identified as one of the key components of innate immunity in vertebrate species and have been reported to be expressed in the reproductive organs in various female species, including the chicken. However, mechanisms of antimicrobial protection of male reproductive organs mediated by TLRs are poorly understood. The objectives of this study were to determine the expression profile of the entire family of the ten chicken TLR genes in the chicken testis, to investigate whether sexual maturation affects their testicular mRNA abundance and to determine the changes in their expression levels in response to Salmonella enteritidis (SE) infection. RNA was extracted from the testis of healthy pre-pubertal, sexually mature and aged birds, and from sexually mature SE infected birds. RT-PCR analysis revealed that all TLRs, apart from TLR1-1 (TLR6), were expressed in the chicken testis. Quantitative real-time PCR analysis revealed that the testicular mRNA abundance of certain TLRs was developmentally regulated with respect to sexual maturation, while SE infection resulted in a significant induction of TLR2-1, 4, 5, 15 and 21 in the testis of sexually mature birds compared, to healthy birds of the same age. These findings provide strong evidence to suggest a key role of TLRs in the innate immune responses of chicken testis against Salmonella colonization.

Toll-like receptors (TLRs) are a critical component of the innate immune response in many vertebrates, including avian species. The recent findings of chicken TLRs (cTLRs) expression in ovarian follicles and in the chicken ovary in vivo, as well as the changes in their expression in response to lipopolysaccharide or Salmonella enteritidis (SE) infection, have broad implications for reproductive physiology and for the prevention of transmission of zoonotic diseases to humans through the consumption of contaminated poultry eggs. Because the main route of egg contamination is from infection of the oviduct and mainly from the vagina, the aim of this study was to investigate the expression of the ten cTLRs identified to date in the chicken oviduct in vivo, to determine whether sexual maturation affects their mRNA abundance and to investigate whether SE infection alters the expression of TLRs in the chicken vagina. RNA was extracted from the vagina of healthy prepubertal, sexually mature and aged birds, and from sexually mature and aged SE infected birds. RT-PCR analysis revealed that all types of cTLRs apart from TLR1-1 were expressed in the vagina of sexually mature birds. Quantitative real-time PCR analysis revealed that the mRNA abundance of TLR2-1, 2-2 and 4 differ with respect to sexual maturation in the chicken vagina. SE infection resulted in a significant induction of TLR5 and 15 in the vagina of sexually mature birds, and in a significant induction of TLR2-1, 4 and 15 in the vagina of aged birds, while a significant down-regulation was observed for TLR7 in the vagina of sexually mature birds. These findings suggest that a TLR mediated immune response mechanism exists in the chicken vagina, playing a crucial role in preventing microbial pathogens from being incorporated into newly forming eggs.

In Greece, up until now, monoculture has been the prevailing fish and shellfish farming system. However, in the last few years, the farmers seek to develop alternative production patterns that could improve the economic performance of their farms through the optimal utilisation of the available resources. The concept of integrated aquaculture is old; however during the last 30 years many authors have suggested various production systems, in which different species are cultured together, in order to reduce the environmental impacts of aquaculture and/or to reduce the feeding cost. In particular, people have attempted to integrate mussel and micro-or macro-algae culture into open-sea or land-based fish farming. When certain criteria are met, such integrated systems could improve the sustainability of the farms, mainly through the reduction of the waste treatment cost and the use of the mussels or the algae in the diet of the farmed fish.