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Antioxidant effect of crocin on bovine sperm quality and in vitro fertilization
- Source :
- Theriogenology. 84(8)
- Publication Year :
- 2015
-
Abstract
- Reactive oxygen species (ROS) production above critical levels affects the genetic and functional integrity of spermatozoa by causing oxidative stress. Spermatozoa are susceptible to oxidative stress in terms of motility and fertilization capacity. Crocin (crocetin di-gentiobiose ester), a main constituent of Crocus Sativus L. (saffron), is known for its antioxidant activity by scavenging ROS, especially superoxide anion. The aim of the present study is to evaluate the effect of crocin on the quality characteristics of spermatozoa and fertilization rate. Frozen-thawed and washed spermatozoa from four different bulls were incubated with three different concentrations of crocin (0.5, 1, and 2 mM), for 120 and 240 minutes, in the presence of a negative control, and were evaluated in terms of motility, viability, acrosomal status, DNA fragmentation index, intracellular ROS, and lipid peroxidation. The most potent concentration of crocin (1 mM) was also added in the fertilization medium to test its impact on fertilization outcome. The results indicate that the incubation of spermatozoa with 1 mM of crocin resulted in a statistically significant lower production of ROS, lower lipid peroxidation and in better maintenance of motility, viability, and acrosomal integrity, with a very small number of fragmented cells, compared to the control and the other treated groups (P
- Subjects :
- Male
Antioxidant
medicine.medical_treatment
Crocetin
Fertilization in Vitro
Phosphatidylserines
Biology
medicine.disease_cause
Antioxidants
Andrology
Crocin
Lipid peroxidation
Toxicology
chemistry.chemical_compound
Human fertilization
Food Animals
medicine
Animals
Small Animals
chemistry.chemical_classification
Cryopreservation
Reactive oxygen species
Equine
Sperm
Carotenoids
Spermatozoa
chemistry
Sperm Motility
Animal Science and Zoology
Cattle
Lipid Peroxidation
Reactive Oxygen Species
Oxidative stress
Semen Preservation
Subjects
Details
- ISSN :
- 18793231
- Volume :
- 84
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- Theriogenology
- Accession number :
- edsair.doi.dedup.....e164ba099f9cf05eabed900205686f4f