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The coordination of progenitor self-renewal, neuronal production, and migration is essential to the normal development and evolution of the cerebral cortex. Numerous studies have shown that the Notch, Wnt/beta-catenin, and Neurogenin pathways contribute separately to progenitor expansion, neurogenesis, and neuronal migration, but it is unknown how these signals are coordinated. In vitro studies suggested that the mastermind-like 1 (MAML1) gene, homologue of the Drosophila mastermind, plays a role in coordinating the aforementioned signaling pathways, yet its role during cortical development remains largely unknown. Here we show that ectopic expression of dominant-negative MAML (dnMAML) causes exuberant neuronal production in the mouse cortex without disrupting neuronal migration. Comparing the transcriptional consequences of dnMAML and Neurog2 ectopic expression revealed a complex genetic network controlling the balance of progenitor expansion versus neuronal production. Manipulation of MAML and Neurog2 in cultured human cerebral stem cells exposed interactions with the same set of signaling pathways. Thus, our data suggest that evolutionary changes that affect the timing, tempo, and density of successive neuronal layers of the small lissencephalic rodent and large convoluted primate cerebral cortex depend on similar molecular mechanisms that act from the earliest developmental stages.

Human higher cognition is attributed to the evolutionary expansion and elaboration of the human cerebral cortex. However, the genetic mechanisms contributing to these developmental changes are poorly understood. We used comparative epigenetic profiling of human, rhesus macaque, and mouse corticogenesis to identify promoters and enhancers that have gained activity in humans. These gains are significantly enriched in modules of coexpressed genes in the cortex that function in neuronal proliferation, migration, and cortical-map organization. Gain-enriched modules also showed correlated gene expression patterns and similar transcription factor binding site enrichments in promoters and enhancers, suggesting that they are connected by common regulatory mechanisms. Our results reveal coordinated patterns of potential regulatory changes associated with conserved developmental processes during corticogenesis, providing insight into human cortical evolution.

Summary Evolutionary elaboration of tissues starts with changes in the genome and location of the stem cells. For example, GABAergic interneurons of the mammalian neocortex are generated in the ventral telencephalon and migrate tangentially to the neocortex, in contrast to the projection neurons originating in the ventricular/subventricular zone (VZ/SVZ) of the dorsal telencephalon. In human and nonhuman primates, evidence suggests that an additional subset of neocortical GABAergic interneurons is generated in the cortical VZ and a proliferative niche, the outer SVZ. The origin, magnitude, and significance of this species-specific difference are not known. We use a battery of assays applicable to the human, monkey, and mouse organotypic cultures and supravital tissue to identify neuronal progenitors in the cortical VZ/SVZ niche that produce a subset of GABAergic interneurons. Our findings suggest that these progenitors constitute an evolutionary novelty contributing to the elaboration of higher cognitive functions in primates.

An odorant receptor map in mammals that is constructed by the glomerular coalescence of sensory neuron axons in the olfactory bulb is essential for proper odor information processing. How this map is linked with olfactory cortex is unknown. Using a battery of methods, including various markers of cell division in combination with tracers of neuronal connections and time-lapse live imaging, we found that early- and late-generated mouse mitral cells became differentially distributed in the dorsal and ventral subdivisions of the odorant receptor map. In addition, the late-generated mitral cells extended substantially stronger projections to the olfactory tubercle than did the early-generated cells. Together, these data indicate that the odorant receptor map is developmentally linked to the olfactory cortices in part by the birthdate of mitral cells. Thus, different olfactory cortical regions become involved in processing information from distinct regions of the odorant receptor map.

Cilia are present on nearly all cell types in mammals and perform remarkably diverse functions. However, the mechanisms underlying ciliogenesis are unclear. Here, we cloned a previously uncharacterized highly conserved gene, stumpy , located on mouse chromosome 7. Stumpy was ubiquitously expressed, and conditional loss in mouse resulted in complete penetrance of perinatal hydrocephalus (HC) and severe polycystic kidney disease (PKD). We found that cilia in stumpy mutant brain and kidney cells were absent or markedly deformed, resulting in defective flow of cerebrospinal fluid. Stumpy colocalized with ciliary basal bodies, physically interacted with γ-tubulin, and was present along ciliary axonemes, suggesting that stumpy plays a role in ciliary axoneme extension. Therefore, stumpy is essential for ciliogenesis and may be involved in the pathogenesis of human congenital malformations such as HC and PKD.

The standard method of detecting neurogenesis uses bromodeoxyuridine (BrdU) to label DNA synthesis followed by double labeling with neuronal markers. However, DNA synthesis may occur in events unrelated to neurogenesis including aneuploidy and abortive cell cycle reentry. Hence, it is important to confirm neurogenesis with methods other than BrdU incorporation. To this end, we have generated transgenic nestin-CreER mice that express tamoxifen-inducible Cre recombinase under the control of a nestin enhancer. When crossed with a ubiquitous Enhanced Green Fluorescent Protein (EGFP)-Cre-reporter line, the bitransgenic animals can reveal the nestin-positive progenitors and their progeny with EGFP after tamoxifen induction. This system has many applications including visualization of embryonic neural progenitors, detection of postnatally transformed radial glial cells, and labeling adult neural progenitors in the subventricular zone (SVZ). To examine the contribution of SVZ progenitors to cell replacement after stroke, tamoxifen-induced mice were challenged with focal ischemia or combined ischemia-hypoxia followed by BrdU injection. This analysis revealed only very few EGFP-positive cells outside the SVZ after focal ischemia but robust DNA synthesis by hippocampal neurons without immediate cell death following ischemia-hypoxia. These results suggest that the nestin-CreER system is a useful tool for detecting embryonic and adult neurogensis. They also confirm the existence of nonproliferative DNA synthesis by old neurons after experimental brain injury.

In contrast to the prenatal development of the cerebral cortex, when cell production, migration, and layer formation dominate, development after birth involves more subtle processes, such as activity-dependent plasticity that includes refinement of synaptic connectivity by its stabilization and elimination. In the present study, we use RNA-seq with high spatial resolution to examine differential gene expression across layers 2/3, 4, 5, and 6 of the mouse visual cortex before the onset of the critical period of plasticity [postnatal day 5 (P5)], at its peak (P26), and at the mature stage (P180) and compare it with the prefrontal association area. We find that, although genes involved in early developmental events such as cell division, neuronal migration, and axon guidance are still prominent at P5, their expression largely terminates by P26, when synaptic plasticity and associated signaling pathways become enriched. Unexpectedly, the gene expression profile was similar in both areas at this age, suggesting that activity-dependent plasticity between visual and association cortices are subject to the same genetic constraints. Although gene expression changes follow similar paths until P26, we have identified 30 regionally enriched genes that are prominent during the critical period. At P180, we identified several hundred differentially expressed gene isoforms despite subsiding levels of gene expression differences. This result indicates that, once genetic developmental programs cease, the remaining morphogenetic processes may depend on posttranslational events.

The function and synaptic connections of neurons in the cerebral cortex are defined by their position. Cortical neurons are not generated locally, but rather originate in the proliferative centers lining the ventricle. After the last mitotic division, newborn neurons migrate between hundreds of micrometers in rodents to several thousand micrometers in humans to achieve their final areal radial and laminar destination (1,2). Despite differences in length and modality of pathways, differential gene activities, and distinct molecular cues involved in guiding each neuronal family to its proper address, the accuracy of the system is a remarkable achievement of evolution. However, when the delivery program goes awry causing neurons to be arrested in ectopic position, the consequences can be focal cortical dysplasias, heterotopias, and other structural malformations. The severity of disruption to cortical modalities depends on the location and expanse of misplaced neurons. For example, malformations in the prefrontal cortex are linked to schizophrenia, whereas malformations in language and limbic areas can cause problems of social behavior or autism spectrum disorders (3–5). Additionally, early disruption to neuronal migration resulting from genetic or environmental causes during the first trimester of human development is more likely to affect deep layer projection neurons. Dysregulation of neuronal migration during the second trimester of human development affects more upper layer neurons. A promising avenue in the quest to determine the genetic basis of schizophrenia has been in bridging the massive information provided by genome-wide association studies, examination of postmortem brain tissue, and molecular genetics approaches in animal models. One gene, among those considered to be implicated in schizophrenia based on its location in the 1q21-q22 locus, is NOS1 adaptor protein (NOS1AP). It interacts with and causes the relocation of nitric oxide synthase (NOS1) to presynaptic terminals. The increased expression of NOS1AP in postmortem tissue of individuals with schizophrenia is thought to disrupt NOS1 interactions with the N-methyl-D-aspartate receptor. Surprisingly, NOS1, the binding partner of NOS1A, is expressed in the prenatal human and rodent brain before synaptic signaling ensues (6). In this issue of Biological Psychiatry, Carrel et al. (7) show the expression of NOS1AP in the frontal lobe of developing rats and that experimentally manipulating the expression level of NOS1AP causes defects in neuronal migration. Using in utero electroporation, which permits accurate spatial and temporal loss of function or gain of function, the authors show that overexpression of NOS1AP disrupts the transition of migrating neurons from the multipolar to bipolar stage in the intermediate zone. The negative effects of excess NOS1AP on neuronal migration can be rescued experimentally by short hairpin RNA–mediated knockdown. By introducing several truncated NOS1AP mutant genes in utero, the authors were able to identify the N-terminal phosphotyrosine-binding

Most neurons in the developing mammalian brain migrate to their final destinations by translocation of the cell nucleus within their leading process and immature bipolar body that is devoid of synaptic connections. Here, we used a combination of immunohistochemistry at light- and electron-microscopic (EM) levels and time-lapse imaging in slice cultures to analyze migration of synaptically interconnected, cholecystokinin-immunopositive [CCK(+)] interneurons in the dentate gyrus in the rat hippocampus during early postnatal ages. We observed dynamic morphogenetic transformation of the CCK(+) interneurons, from a horizontal bipolar shape situated in the molecular layer, through a transitional triangular and then vertical bipolar form that they acquire while traversing the granular layer to finally assume an adult-like pyramidal-shaped morphology on entering the hilus. Immunostaining with anti-glial fibrillary acidic protein and three-dimensional reconstructions from serial EM images indicate that, unlike granule cells, which migrate from the hilus to the granular layer, interneurons traverse this layer in the opposite direction without apparent surface-mediated guidance of the radial glial cells. Importantly, the somas, dendrites, and axons of the CCK(+) transitional forms maintain old and acquire new synaptic contacts while migrating across the dentate plate. The migration of synaptically interconnected neurons that may occur in response to local functional demand represents a novel mode of cell movement and form of neuroplasticity.

The proliferative ventricular zone (VZ) is the main source of projection neurons for the overlying cerebral neocortex. The number and diversity of neocortical neurons is determined, in part, by factors controlling the proliferation and specification of VZ cells during embryonic development. We used a variety of methods, includingin uteroelectroporation with specific cellular markers, computer-assisted serial EM cell reconstruction, and time-lapse multiphoton imaging to characterize the molecular and morphological characteristics of the VZ constituents and to capture their behavior during cell division. Our analyses reveal at least two types of dividing cells in the VZ: (1) radial glial cells (RGCs) that span the entire neocortical wall and maintain contact both at the ventricular and pial surfaces throughout mitotic division, and (2) short neural precursors (SNPs) that possess a ventricular endfoot and a basal process of variable length that is retracted during mitotic division. These two precursor cell classes are present concomitantly in the VZ, but their relative number changes over the course of cortical neurogenesis. Moreover, the SNPs are morphologically, ultrastructurally and molecularly distinct from dividing RGCs. For example, SNPs are marked by their preferential expression of the tubulin α-1 promoter whereas RGCs instead express the glutamate–aspartate transporter and brain lipid binding protein promoters. In contrast to recent studies that suggest that RGCs are the sole type of VZ precursor, the present study indicates that the VZ in murine dorsal telencephalon is similar to that in human and nonhuman primates, because it contains multiple types of neuronal precursors.

The development of the cerebellar cortex depends on intrinsic genetic programs and orchestrated cell-cell/cell-matrix interactions. Matrix metalloproteinases (MMPs) are proteolytic enzymes that play an important role in these interactions. MMP-2 and MMP-9 are involved in diverse neuronal functions including migration, process extension, and synaptic plasticity. We investigated the spatiotemporal pattern of expression/activity of MMP-2/MMP-9 in the developing cerebellum and their role in the histogenesis of the cerebellar cortex. The levels of transcripts of MMP-2/MMP-9 were measured with real-time quantitative polymerase chain reaction. An initial decrease in MMP-2/MMP-9 transcripts was observed between postnatal days 3 (PD3) and PD6, and the mRNA levels remained relatively constant thereafter. Zymographic analysis revealed that the expression/activity of MMP-2/MMP-9 persisted longer than their transcripts; the downregulation occurred around PD9, suggesting a mechanism of translational or post-translational regulation. The gelatinase activity was localized in the external granule layer (EGL) and the internal granule layer during PD3-PD12. The immunoreactivity of MMP-2 was mainly localized in the EGL, the Bergmann glial fibers, and the Purkinje cell layer (PCL), whereas MMP-9 immunoreactivity was detected intensively in the PCL and the extracellular space of the molecular layer. Expression of MMP-9 was relatively weak in the EGL. The immunoreactivity of MMP-2/MMP-9 became undetectable after PD21. A similar expression pattern of MMP-2/MMP-9 was observed in organotypic cerebellar slice cultures. Exposure of organotypic slices to a specific MMP-2/MMP-9 inhibitor significantly increased the thickness of the EGL and concurrently decreased the number of migrating granule neurons in the molecular layer. Thus, MMP-2 and MMP-9 play a role in the postnatal cerebellar morphogenesis.

Publisher Summary This chapter reviews that structural plasticity of astrocytes in the normally functioning brain is widespread. Two of the best-studied regions where structural plasticity occurs are the supraoptic nucleus (SON) and posterior pituitary. It discusses the recent developments in understanding of how this structural remodelling, including new finding that show microglia also participate in structural plasticity of the SON. The chapter also introduces other brain areas, where structural plasticity of astrocytes has been demonstrated. Structural plasticity of glia, predominantly that of astrocytes, occurs throughout the nervous system. Sites, where structural plasticity of astrocytes has been documented in normal brains include the hippocampus with long term potentiation induction in the suprachiasmatic nucleus across the diurnal cycle, in the cerebellar cortex with motor learning in the arcuate nucleus of the rat and monkey, across changing estrogen levels, and extensively, in the hypothalamo-neurohypophyseal system (HNS) in response to diverse conditions leading to the synthesis and release of the peptides oxytocin (OX) and vasopressin (VP). The chapter concludes that the factors that are investigated in the SON of the hypothalamus include reorientation of astrocytes, reentry of glial cells into the cell cycle, downregulation of morphoregulatory molecules, dissolution of the basal lamina, changes in the GFAP, concomitant changes in expression of GFAP message, and changes in the population of resident microglia.