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Flexible and dispatchable, high-efficiency power generation supplied with carbon-neutral renewable fuels is needed to help enable defossilization of the electric grid. Pressurized, hybrid SOFC systems fueled with hydrogen, biogas, or renewable natural gas can generate clean power at ultra-high efficiency. In this summary, we provide an update on the development progress of a full-scale, hybrid system that targets low cost (

The development of commercial SOFC systems often utilizes models of a single representative SOFC stack to simulate the system’s prime mover. In practice, however, these representative single-stack SOFC models fail to consider effects due to the reality of multi-stack power modules needed to construct commercial-scale systems. Effects such as flow maldistribution, inter-stack heat transfer, and area-specific resistance (ASR) variation between stack units within a single module will create deviations in performance from a single stack-based system simulation. The following outlines efforts to consider these thermofluidic effects and the associated performance deviations of a novel 30kWe SOFC multi-stack module using models in the gPROMS Modelbuilder® environment. Specifically, the impacts of gas manifolding, heat loss, and stack placement are investigated, and their potential impacts are discussed.

Direct numerical simulations (DNS) at bulk Reynolds number Re $$=10^4$$ = 10 4 and bulk Richardson number Ri $$=0.25$$ = 0.25 of plane Couette flow are performed with the results used to analyze the structure and mixing intensity in strongly stable boundary-layer flows. The Couette flow set-up is used as a proxy for a real-world stable boundary layer flow with surface thermal heterogeneity. Along the upper and lower walls, the temperature is either homogeneous or varies sinusoidally, but the horizontal-mean surface temperature is the same in all cases. Over homogeneous surfaces, the strong stratification always quenches turbulence resulting in linear velocity and temperature profiles. However, over a heterogeneous surface turbulence survives. Molecular diffusion and turbulence contribute to down-gradient momentum transfer. The total (diffusive plus turbulent) heat flux is directed downward, but its turbulent contribution is positive, i.e., up the mean temperature gradient. Analysis of covariances of velocity and temperature, their skewness, and the flow structure suggests that counter-gradient heat transport is due to quasi-organized cell-like vortical motions generated by surface thermal heterogeneity. These motions transfer heat upwards similar to their counterparts in highly convective boundary layers. Thus, the flow over heterogeneous surface features local convective instabilities and upward eddy heat transport, although the overall stratification remains stable with downward mean heat transfer. The DNS results are compared to the results from large-eddy simulations of weakly stable boundary layers (Mironov and Sullivan in J Atmos Sci 73:449–464, 2016). The DNS findings corroborate the key role of temperature variance in setting the structure and transport properties of stably stratified flow over heterogeneous surfaces, and the importance of third-order transport of the temperature variance.

In the present study, we used an experimental paradigm with subjective and objective assessments to further explicate the role of positive emotion dysregulation on risky behavior. Participants were 151 community women currently experiencing intimate partner violence and using substances (age: M = 40.81 years; 30.5% Black). Participants were randomly assigned to positive, negative, and neutral idiographic emotion inductions. Subjective (state self-report) and objective (high-frequency heart rate variability [hfHRV], skin conductance response, and salivary cortisol) markers of emotion dysregulation were assessed, following which participants completed subjective (state urges for substances) and objective (Balloon Analogue Risk Task) measures of risky behavior. Results showed that (a) greater self-reported state emotion dysregulation and lower hfHRV predicted more urges for substances in the positive (vs. negative and neutral) emotion-induction condition and (b) lower hfHRV predicted more behavioral risk-taking propensity in the positive (vs. neutral) emotion-induction condition. These findings provide additional support for the influence of positive emotion dysregulation on risky behavior.

We investigate how four different processing routes influence the grain boundary chemistry of the proton-conducting oxide BaZr0.9Y0.1O3−δ (BZY10) via atom probe tomography (APT). Differing processing routes can cause large variations in GB chemistry.

Substitution of a labile Cl− for an NHC ligand in DNA-intercalating [Pt(terpyridine)]+ complexes improved the cytotoxicity and cellular accumulation, while in vivo studies indicated good tolerability in zebrafish.

Using OH reactivity we assess the major daytime OH radical sinks in western U.S. wildfire plumes and other smoke impacted environments, testing their current model representation while providing a roadmap for future model development.

Ambient humidity plays a key role in the health and well-being of us and our surroundings, making it necessary to carefully monitor and control it. To achieve this goal, several types of instruments based on various materials and operating principles have been developed. Reducing the production costs for such systems without affecting their sensitivity and reliability would allow for broader use and greater efficiency. Organic materials are prime candidates for incorporation in humidity sensors given their extraordinary chemical diversity, low cost, and ease of processing. Here, we designed, assembled and tested humidity sensors based on molecular rectifiers that can electrically transduce the changes in the ambient humidity to offer accurate quantitative information in the range of 0 to 70% relative humidity. Their operation relies on the changes occurring in the electric field experienced by the molecular layer upon absorption of the polar water molecules, resulting in modifications in the height and shape of the tunneling barrier. The response is reversible and reproducible upon multiple cycles and, coupled with the simplicity of the device architecture and manufacturing, makes these nanoscale sensors attractive for incorporation in various applications.

Current submesoscale restratification parameterizations, which help set mixed layer depth in global climate models, depend on a simplistic scaling of frontal width shown to be unreliable in several circumstances. Observations and theory indicate that frontogenesis is common, but stable frontal widths arise in the presence of turbulence and instabilities that participate in keeping fronts at the scale observed, the arrested scale. Here we propose a new scaling law for arrested frontal width as a function of turbulent fluxes via the turbulent thermal wind (TTW) balance. A variety of large-eddy simulations (LES) of strain-induced fronts and TTW-induced filaments are used to evaluate this scaling. Frontal width given by boundary layer parameters drawn from observations in the General Ocean Turbulence Model (GOTM) are found qualitatively consistent with the observed range in regions of active submesoscales. The new arrested front scaling is used to modify the mixed layer eddy restratification parameterization commonly used in coarse-resolution climate models. Results in CESM-POP2 reveal the climate model’s sensitivity to the parameterization update and changes in model biases. A comprehensive multimodel study is in planning for further testing. Significance Statement The ocean surface plays a major role in the climate system, primarily through exchange in properties, such as in heat and carbon, between the ocean and atmosphere. Accurate model representation of ocean surface processes is crucial for climate simulations, yet they tend to be too small, fast, or complex to be resolved. Significant efforts lie in approximating these small-scale processes using reduced expressions that are solved by the model. This study presents an improved representation of the ocean surface in climate models by capturing some of the synergy that has been missing between the processes that define it. Results encourage further testing across a wider range of models to comprehensively evaluate the effects of this adjustment in climate simulations.

A persistent spatial organization of eddies is identified in the lowest portion of the stably-stratified planetary boundary layer. The analysis uses flow realizations from published large-eddy simulations (Sullivan et al., J Atmos Sci 73(4):1815-1840, 2016) ranging in stability from neutral to nearly z-less stratification. The coherent turbulent structure is well approximated as a series of uniform momentum zones (UMZs) and uniform temperature zones (UTZs) separated by thin layers of intense gradients that are significantly greater than the mean. This pattern yields stairstep-like instantaneous flow profiles whose shape is distinct from the mean profiles that emerge from long-term averaging. However, the scaling of the stairstep organization is closely related to the resulting mean profiles. The differences in velocity and temperature across the thin gradient layers remain proportional to the surface momentum and heat flux conditions regardless of stratification. The vertical thickness of UMZs and UTZs is proportional to height above the surface for neutral and weak stratification, but becomes thinner and less dependent on height as the stability increases. Deviations from the logarithmic mean profiles for velocity and temperature observed under neutral conditions are therefore predominately due to the reduction in zone size with increasing stratification, which is empirically captured by existing Monin-Obukhov similarity relations for momentum and heat. The zone properties are additionally used to explain trends in the turbulent Prandtl number, thus providing a connection between the eddy organization, mean profiles, and turbulent diffusivity in stably stratified conditions., Comment: 35 pages, 12 figures

What is the central question of this study? Skeletal muscle extracellular vesicles likely act as pro-angiogenic signalling factors: does overexpression of peroxisome proliferator-activated receptor γ coactivator 1-α (PGC-1α) alter skeletal muscle myotube extracellular vesicle release, contents and angiogenic potential? What is the main finding and its importance? Overexpression of PGC-1α results in secretion of extracellular vesicles that elevate measures of angiogenesis and protect against acute oxidative stress in vitro. Skeletal muscle with high levels of PGC-1α expression, commonly associated with exercise induced angiogenesis and high basal capillarization, may secrete extracellular vesicles that support capillary growth and maintenance.Skeletal muscle capillarization is proportional to muscle fibre mitochondrial content and oxidative capacity. Skeletal muscle cells secrete many factors that regulate neighbouring capillary endothelial cells (ECs), including extracellular vesicles (SkM-EVs). Peroxisome proliferator-activated receptor γ coactivator 1-α (PGC-1α) regulates mitochondrial biogenesis and the oxidative phenotype in skeletal muscle. Skeletal muscle PGC-1α also regulates secretion of multiple angiogenic factors, but it is unknown whether PGC-1α regulates SkM-EV release, contents and angiogenic signalling potential. PGC-1α was overexpressed via adenovirus in primary human myotubes. EVs were collected from PGC-1α-overexpressing myotubes (PGC-EVs) as well as from green fluorescent protein-overexpressing myotubes (GFP-EVs), and from untreated myotubes. EV release and select mRNA contents were measured from EVs. Additionally, ECs were treated with EVs to measure angiogenic potential of EVs in normal conditions and following an oxidative stress challenge. PGC-1α overexpression did not impact EV release but did elevate EV content of mRNAs for several antioxidant proteins (nuclear factor erythroid 2-related factor 2, superoxide dismutase 2, glutathione peroxidase). PGC-EV treatment of cultured human umbilical vein endothelial cells (HUVECs) increased their proliferation (+36.6%), tube formation (length: +28.1%; number: +25.7%) and cellular viability (+52.9%), and reduced reactive oxygen species levels (-41%) compared to GFP-EVs. Additionally, PGC-EV treatment protected against tube formation impairments and induction of cellular senescence following acute oxidative stress. Overexpression of PGC-1α in human myotubes increases the angiogenic potential of SkM-EVs. These angiogenic benefits coincided with increased anti-oxidative capacity of recipient HUVECs. High PGC-1α expression in skeletal muscle may prompt the release of SkM-EVs that support vascular redox homeostasis and angiogenesis.

Heteromormyrus Steindachner, 1866, a genus of Mormyridae (Teleostei: Osteoglossomorpha), has been monotypic since the description of Heteromormyrus pauciradiatus (Steindacher, 1866) from a single specimen. No type locality other than “Angola” was given and almost no specimens have been subsequently identified to this species. In order to investigate the relationship of this taxon to fresh specimens collected in Angola and elsewhere, whole genome paired-end sequencing of DNA extracted from the holotype specimen of Heteromormyrus pauciradiatus was performed and a nearly complete mitogenome assembled from the sequences obtained. Comparison of cytochrome oxidase I and cytochrome b sequences from this mitogenome to sequences from recently collected material reveal that Heteromormyrus pauciradiatus is closely related to specimens identified as Hippopotamyrus ansorgii (Boulenger, 1905), Hippopotamyrus szaboi Kramer, van der Bank & Wink, 2004, Hippopotamyrus longilateralis Kramer & Swartz, 2010, as well as to several undescribed forms from subequatorial Africa collectively referred to in the literature as the “Hippopotamyrus ansorgii species complex” and colloquially known as “slender stonebashers.” Previous molecular phylogenetic work has shown that these species are not close relatives of Hippopotamyrus castor Pappenheim, 1906, the type species of genus Hippopotamyrus Pappenheim, 1906 from Cameroon, and are thus misclassified. Hippopotamyrus ansorgii species complex taxa and another species shown to have been misclassified, Paramormyrops tavernei (Poll, 1972), are placed in genus Heteromormyrus and one genetic lineage from the Kwanza and Lucala rivers of Angola are identified as conspecific Heteromormyrus pauciradiatus. Three additional new combinations and a synonymy in Mormyridae are introduced. The morphological characteristics and geographical distribution of the genus Heteromormyrus are reviewed. The electric organ discharges (EODs) of Heteromormyrus species are to be treated in a separate study.

Carlsbad Caverns National Park in southeastern New Mexico is adjacent to the Permian Basin, one of the most productive oil and gas regions in the country. The 2019 Carlsbad Caverns Air Quality Study (CarCavAQS) was designed to examine the influence of regional sources, including urban emissions, oil and gas development, wildfires, and soil dust on air quality in the park. Field measurements of aerosols, trace gases, and deposition were conducted from 25 July through 5 September 2019. Here, we focus on observations of fine particles and key trace gas precursors to understand the important contributing species and their sources and associated impacts on haze. Key gases measured included aerosol precursors, nitric acid and ammonia, and oil and gas tracer, methane. High-time resolution (6-min) PM2.5 mass ranged up to 31.8 µg m−3, with an average of 7.67 µg m−3. The main inorganic ion contributors were sulfate (avg 1.3 µg m−3), ammonium (0.30 µg m−3), calcium (Ca2+) (0.22 µg m−3), nitrate (0.16 µg m−3), and sodium (0.057 µg m−3). The WSOC concentration averaged 1.2 µg C m−3. Sharp spikes were observed in Ca2+, consistent with local dust generation and transport. Ion balance analysis and abundant nitric acid suggest PM2.5 nitrate often reflected reaction between nitric acid and sea salt, forming sodium nitrate, and between nitric acid and soil dust containing calcium carbonate, forming calcium nitrate. Sulfate and soil dust are the major contributors to modeled light extinction in the 24-hr average daily IMPROVE observations. Higher time resolution data revealed a maximum 1-hr extinction value of 90 Mm−1 (excluding coarse aerosol) and included periods of significant light extinction from BC as well as sulfate and soil dust. Residence time analysis indicated enrichment of sulfate, BC, and methane during periods of transport from the southeast, the direction of greatest abundance of oil and gas development. Implications: Rapid development of U.S. oil and gas resources raises concerns about potential impacts on air quality in National Parks. Measurements in Carlsbad Caverns National Park provide new insight into impacts of unconventional oil and gas development and other sources on visual air quality in the park. Major contributors to visibility impairment include sulfate, soil dust (often reacted with nitric acid), and black carbon. The worst periods of visibility and highest concentrations of many aerosol components were observed during transport from the southeast, a region of dense Permian Basin oil and gas development.

Light absorbing organic carbon, or brown carbon (BrC), can be a significant contributor to the visible light absorption budget. However, the sources of BrC and the contributions of BrC to light absorption are not well understood. Biomass burning is thought to be a major source of BrC. Therefore, as part of the WE-CAN (Western Wildfire Experiment for Cloud Chemistry, Aerosol Absorption and Nitrogen) study, BrC absorption data were collected on board the National Science Foundation/National Center for Atmospheric Research (NSF/NCAR) C-130 aircraft as it intercepted smoke from wildfires in the western US in July–August 2018. BrC absorption measurements were obtained in near real-time using two techniques. The first coupled a particle-into-liquid sampler (PILS) with a liquid waveguide capillary cell and a total organic carbon analyzer for measurements of water-soluble BrC absorption and WSOC (water-soluble organic carbon). The second employed a custom-built photoacoustic aerosol absorption spectrometer (PAS) to measure total absorption at 405 and 660 nm. The PAS BrC absorption at 405 nm (PAS total Abs 405 BrC) was calculated by assuming the absorption determined by the PAS at 660 nm was equivalent to the black carbon (BC) absorption and the BC aerosol absorption Ångström exponent was 1. Data from the PILS and PAS were combined to investigate the water-soluble vs. total BrC absorption at 405 nm in the various wildfire plumes sampled during WE-CAN. WSOC, PILS water-soluble Abs 405, and PAS total Abs 405 tracked each other in and out of the smoke plumes. BrC absorption was correlated with WSOC (R2 value for PAS =0.42 and PILS =0.60) and CO (carbon monoxide) (R2 value for PAS =0.76 and PILS =0.55) for all wildfires sampled. The PILS water-soluble Abs 405 was corrected for the non-water-soluble fraction of the aerosol using the calculated UHSAS (ultra-high-sensitivity aerosol spectrometer) aerosol mass. The corrected PILS water-soluble Abs 405 showed good closure with the PAS total Abs 405 BrC with a factor of ∼1.5 to 2 difference. This difference was explained by particle vs. bulk solution absorption measured by the PAS vs. PILS, respectively, and confirmed by Mie theory calculations. During WE-CAN, ∼ 45 % (ranging from 31 % to 65 %) of the BrC absorption was observed to be due to water-soluble species. The ratio of BrC absorption to WSOC or ΔCO showed no clear dependence on fire dynamics or the time since emission over 9 h.

115 p.-4 fig.-45 fig. supl.-14 tab supl., Mixed plastics waste represents an abundant and largely untapped feedstock for the production of valuable products. The chemical diversity and complexity of thesematerials, however, present major barriers to realizing this opportunity. In this work, we show that metal-catalyzed autoxidation depolymerizes comingled polymers into a mixture of oxygenated small molecules that are advantaged substrates for biological conversion. We engineer a robust soil bacterium, Pseudomonas putida, to funnel these oxygenated compounds into a single exemplary chemical product, either b-ketoadipate or polyhydroxyalkanoates. This hybrid process establishes a strategy for the selective conversion of mixed plastics waste into useful chemical products., Funding was provided by the US Department of Energy, Office of Energy Efficiency and Renewable Energy, Advanced Manufacturing Office (AMO), and Bioenergy Technologies Office (BETO). This work was performed as part of the BOTTLE Consortium and was supported by AMO and BETO under contract no. DE-AC36- 08GO28308 with the National Renewable Energy Laboratory (NREL),operated by the Alliance for Sustainable Energy, LLC. The BOTTLE Consortium includes members from MIT, funded under contract no. DE-AC36-08GO28308 with NREL. Contributions by S.S.S. were supported by the US Department of Energy, Office of Basic Energy Sciences, under award no. DEFG02-05ER15690.

Responses of forest-floor small mammals to clearcutting are species-specific with generalists occupying a range of habitats, and specialists persisting on clearcuts for variable periods. We investigated the responses in abundance and species composition of small mammal communities to cumulative clearcutting of coniferous forests on a landscape that had four independent clearcutting events (Periods 1 to 4) over a 42-year interval from 1979 to 2020 in south-central British Columbia, Canada. We ask if the small mammal communities have changed significantly over these decades owing to removal of old-growth forest by clearcut harvesting. Hypotheses (H) predicted that the small mammal community would (H1) increase in abundance, species richness, and diversity on new clearcuts owing to the availability of early seral post-harvest habitats from cumulative clearcutting; and (H2) have higher mean abundance, species richness, and species diversity in clearcut than uncut forest sites, owing to availability of vegetative food and cover. A third hypothesis (H3) predicted that abundance of (i) early seral vegetation (herbs and shrubs) and (ii) small mammal populations, will be greater in ungrazed clearcut sites than in those grazed by cattle (Bos taurus). Mean total numbers of small mammals on new clearcuts declined in Periods 3 and 4, and hence did not support the abundance part of H1. Much of this decline was owing to low numbers of the long-tailed vole (Microtus longicaudus) and meadow vole (M. pennsylvanicus). Two generalist species: the deer mouse (Peromyscus maniculatus) and northwestern chipmunk (Neotamias amoenus), contributed to high mean species richness and diversity in Periods 2 and 3 before these metrics declined in Period 4, and hence partly supported H1. The similarity in mean total numbers of small mammals in Periods 2 to 4 did not support the abundance prediction of H2 that total numbers would be higher in clearcut than uncut forest sites. Higher mean species richness (Periods 2 and 3) and diversity (Period 3) measurements on clearcut than forest sites, particularly in the early post-harvest years, did support these parts of H2. The vegetation part (i) of H3 was not supported for herbaceous plants but it was for shrubs. The small mammal part (ii) of H3 that populations would be higher in ungrazed than grazed clearcut sites was supported for abundance but not for species richness or diversity. The decline and near disappearance of both species of Microtus was possibly related to the reduction in plant community abundance and structure from grazing (at least for shrubs) and potentially from drought effects associated with climate change. Loss of microtines from these early seral ecosystems may have profound negative effects on various ecological functions and predator communities.

Imagery and numerical modeling show an abundance of submesoscale oceanic eddies in the upper ocean. Large-eddy simulation (LES) is used to elucidate eddy impacts on the atmospheric boundary layer (ABL) forced by winds, convection, and an eddy with varying radius; the maximum azimuthal eddy speed is 1 m s−1. Simulations span the unstable regime −1/L = [0, ∞], where L is the Monin–Obukhov (M–O) stability parameter. A linearized Ekman model and the LES couple ABL winds to an eddy through rough-wall M–O boundary conditions. The eddy currents cause a surface stress anomaly that induces Ekman pumping in a dipole horizontal pattern. The dipole is understood as a consequence of surface winds aligned or opposing surface currents. In free convection a vigorous updraft is found above the eddy center and persists over the ABL depth. Heterogeneity in surface temperature flux is responsible for the full ABL impact. With winds and convection, current stress coupling generates a dipole in surface temperature flux even with constant sea surface temperature. Wind, pressure, and temperature anomalies are sensitive to an eddy under light winds. The eddy impact on ABL secondary circulations is on the order of the convective velocity scale but grows with increasing current speed, decreasing wind, or increasing convection. Flow past an isolated eddy develops a coherent ABL “wake” and secondary circulations for at least five eddy radii downwind. Kinetic energy exchanges by wind work indicate an eddy-killing effect on the oceanic eddy current, but only a spatial rearrangement of the atmospheric wind work.

Over the past seven decades since the advent of artificial intelligence (AI) technology, researchers have demonstrated and deployed systems incorporating AI in various domains. The absence of model explainability in critical systems such as medical AI and credit risk assessment among others has led to neglect of key ethical and professional principles which can cause considerable harm. With explainability methods, developers can check their models beyond mere performance and identify errors. This leads to increased efficiency in time and reduces development costs. The article summarizes that steering the traditional AI systems toward responsible AI engineering can address concerns raised in the deployment of AI systems and mitigate them by incorporating explainable AI methods. Finally, the article concludes with the societal benefits of the futuristic AI systems and the market shares for revenue generation possible through the deployment of trustworthy and ethical AI systems.

In the United States, the use of pesticide-coated seeds in agriculture is a widely adopted practice. Consumption of treated crop seeds is a possible route of exposure of birds to pesticides. A combination of agronomic and bird life history information is used to identify those species most likely to consume freshly planted corn seeds. Species listed as threatened or endangered by the US Fish and Wildlife Service that could consume treated seeds are also identified. Corn-growing counties in the continental United States were compiled for the years 2017-2020. Seven hundred and eight bird species occur in corn-growing counties. Feeding habits and habitats of North American bird species that occur in corn-growing counties indicate that 107 species could feed on the ground in or adjacent to freshly planted corn fields and consume seeds. Of these, 21 species show a high tendency to feed in or around freshly planted corn fields and could consume a large quantity of corn seeds. Of the 107 species with the potential to consume freshly planted corn, only two full species (as opposed to subspecies), the Gunnison sage-grouse and whooping crane, are listed as threatened or endangered under the US Endangered Species Act. Additionally, two subspecies, Mississippi sandhill crane and masked bobwhite, are listed as endangered. The streaked horned lark is listed as threatened. Of the 21 species, including subspecies, that have the greatest potential to consume a freshly planted treated corn seed, the Mississippi sandhill crane is the only listed species considered to have a high potential to consume treated corn seeds. Therefore, the potential exposure of federally listed threatened or endangered bird species to pesticides through the direct consumption of pesticide-treated corn seeds is low, with the possible exception of the Mississippi sandhill crane. Integr Environ Assess Manag 2022;00:1-13. © 2022 SETAC.

Violent conflicts involving non-State armed groups challenge conventional perceptions of war and armed conflict. Criminal enterprises (transnational organized criminal groups including gangs and cartels) are involved in violent competition for both profit and territorial control in many parts of the world. This paper examines the situation in Mexico and Brazil as case studies to assess the legal challenges to criminal armed violence when criminal groups battle among themselves and the State. The paper focuses on the operational challenges and considerations facing police, military, and security forces and justice institutions to illuminate the legal challenges.

We investigated population responses of Microtus longicaudus to cumulative clear-cutting of coniferous forests and to enhanced understory vegetation in young, fertilized pine stands near Summerland, British Columbia, Canada. We explored if there was a threshold level of habitat quality arising from a given forest disturbance for M. longicaudus to increase to high population levels and potentially fluctuate in abundance over time. Secondly, we asked if these outbreaks were site-specific or part of a long-term pattern. We tested three hypotheses (H) that populations of M. longicaudus would increase in abundance and potentially fluctuate owing to (H1) the availability of early seral postharvest habitats associated with cumulative clear-cut harvesting; (H2) woody debris piles on clear-cuts; and (H3) have higher mean abundance, reproduction, and survival in fertilized forest sites with enhanced understory vegetation. Mean annual and peak abundances of M. longicaudus were significantly different across the four Periods of cumulative forest harvesting with numbers being highest in the first two Periods. Thus, H1, that long-tailed voles would increase in abundance on new clear-cuts, was partially supported for the first two Periods but not in the later Periods. Constant cattle (Bos taurus) grazing during summer periods over the four decades may have reduced vegetative productivity for voles and damped out population responses on these clear-cut sites, at least in the last two Periods. Woody debris piles on clear-cuts may have increased abundance and generated a population fluctuation, thereby supporting H2. Enhanced abundance of understory vegetation had no effect on mean abundance, reproduction, or survival of M. longicaudus, and hence did not support H3. We conclude that it is site-specific disturbances, particularly in forest management, that generate occasional outbreaks of M. longicaudus, and there does not appear to be any long-term pattern to these discordant fluctuations.

We report two cases of middle-aged men who presented with clinical features that satisfied the diagnostic criteria for multisystem inflammatory syndrome in adults (MIS-A). Both patients were treated for toxic shock syndrome and MIS-A and have recovered. The purpose of this article is to communicate our experience and challenges of assessing and treating this condition and to raise awareness of the condition.

A fast-growing body of literature linking emotion dysregulation to substance use has almost exclusively relied on cross-sectional designs and has generally failed to assess dysregulation stemming from positive emotions. The current study measured the momentary associations between both negative and positive emotion dysregulation and substance use, as well as the moderating role of post-traumatic stress disorder (PTSD).Micro-longitudinal momentary data were collected three times per day for 30 days using phone-based interactive voice recording.Northeastern United States.A total of 145 community women (M, age = 40.56, 40.3% white) experiencing intimate partner violence (IPV) and using substances were recruited from 2018 until 2020.Momentary surveys assessed negative and positive emotion dysregulation and alcohol, drug, and cigarette use. PTSD diagnosis was derived from a structured diagnostic interview.Between-person relations that remained significant after covariate adjustment and correction for multiple testing were found for negative emotion dysregulation and both number of drinks (internal rate of return [IRR] = 1.45; 95% CI = 1.07, 1.93; β = 0.37) and drug use (OR = 3.78; 95% CI = 1.86, 7.69; β = 1.33), and positive emotion dysregulation and number of cigarettes (OR = 1.40; 95% CI = 1.07, 1.82; β = 0.34). Of six interactions tests, PTSD was only shown to moderate the within-person relation between positive emotion dysregulation and drug use, even after controlling for relevant covariates and adjusting for multiple testing (OR = 1.26; 95% CI = 1.04, 1.51; β = 0.23), such that women experiencing intimate partner violence with versus without PTSD were more likely to use drugs when experiencing periods of elevated positive emotion dysregulation.Women experiencing intimate partner violence with higher levels of negative dysregulation relative to others may use more alcohol and be more likely to use drugs. Women experiencing intimate partner violence with higher levels of positive dysregulation relative to others may use more cigarettes. Women experiencing intimate partner violence who also have posttraumatic stress disorder may be more likely to use drugs when experiencing momentary elevated levels of positive emotion dysregulation than those who do not also have posttraumatic stress disorder.

Quantitative nucleic acid amplification tests (qNAATs) are critical in treating infectious diseases, such as in HIV viral load monitoring or SARS-CoV-2 testing, in which viral load indicates viral suppression or infectivity. Quantitative PCR is the gold standard tool for qNAATs; however, there is a need to develop point-of-care (POC) qNAATs to manage infectious diseases in outpatient clinics, low- and middle-income countries, and the home. Isothermal amplification methods are an emerging tool for POC NAATs as an alternative to traditional PCR-based workflows. Previous works have focused on relating isothermal amplification bulk fluorescence signals to input copies of target nucleic acids for sample quantification with limited success. In this work, we show that recombinase polymerase amplification (RPA) reactions on paper membranes exhibit discrete fluorescent amplification nucleation sites. We demonstrate that the number of nucleation sites can be used to quantify HIV-1 DNA and RNA in less than 20 minutes. An image-analysis algorithm quantifies nucleation sites and determines the input nucleic acid copies in the range of 67-3,000 copies per reaction. We demonstrate a mobile phone-based system for image capture and onboard processing, illustrating that this method may be used at the point-of-care for qNAATs with minimal instrumentation.

The ability to control machines and equipment with just one's thoughts is a concept that has been explored in the field of neuroscience for decades. The development of brain-computer interfaces (BCIs) has made it possible to translate electrical signals generated by the brain into machine commands. In recent years, the field of BCI has made significant advancements in non-intrusive technology, which has the potential to revolutionize the way we interact with machines and equipment in a variety of fields. The objective of this project is to merge non-intrusive BCI technology with personal protective equipment (PPE) and control the flow of fluids through human sensory and peripheral signals in field environments. A revolutionary system application was developed and tested by paper authors Kyle W. Divine, Thomas P. Sullivan, Nawaf A. Alrajeh and Nabil M. Bukhari from Saudi Aramco. This proposed system involves the use of a BCI with Electroencephalography (EEG) mounted within a traditional hardhat. The electrical signals generated from the thoughts of the wearer are used to train a Convolutional Neural Network (CNN) and modeled to recognize the wearer's imagined words, primarily open, close, push, pull, turn left, or turn right. These interpreted electrical signals are then relayed to the valve through a Micro Controller device resulting in the operation of the valve and the successful control of fluids with simple thought commands.

Table 4 Excel Document

Supplemental Figures 1-8, Supplemental Tables 1-3

Natural killer (NK) cells are a promising cellular therapy for cancer, with challenges in the field including persistence, functional activity, and tumor recognition. Briefly, priming blood NK cells with recombinant human (rh)IL-12, rhIL-15, and rhIL-18 (12/15/18) results in memory-like NK cell differentiation and enhanced responses against cancer. However, the lack of available, scalable Good Manufacturing Process (GMP)–grade reagents required to advance this approach beyond early-phase clinical trials is limiting. To address this challenge, we developed a novel platform centered upon an inert tissue factor scaffold for production of heteromeric fusion protein complexes (HFPC). The first use of this platform combined IL-12, IL-15, and IL-18 receptor engagement (HCW9201), and the second adds CD16 engagement (HCW9207). This unique HFPC expression platform was scalable with equivalent protein quality characteristics in small- and GMP-scale production. HCW9201 and HCW9207 stimulated activation and proliferation signals in NK cells, but HCW9207 had decreased IL-18 receptor signaling. RNA sequencing and multidimensional mass cytometry revealed parallels between HCW9201 and 12/15/18. HCW9201 stimulation improved NK cell metabolic fitness and resulted in the DNA methylation remodeling characteristic of memory-like differentiation. HCW9201 and 12/15/18 primed similar increases in short-term and memory-like NK cell cytotoxicity and IFNγ production against leukemia targets, as well as equivalent control of leukemia in NSG mice. Thus, HFPCs represent a protein engineering approach that solves many problems associated with multisignal receptor engagement on immune cells, and HCW9201-primed NK cells can be advanced as an ideal approach for clinical GMP-grade memory-like NK cell production for cancer therapy.

Legends for Supplementary Figures 1-8 and Supplementary Tables 1-5

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GBM8 and GBM24 growth in culture.

Tumor genotypes and CTC frequency

DNA-FISH analysis of non-EGFR-amplified GBM CTCs.

Immunohistologic and DNA-FISH analysis of tumor cells and CTCs from a patient with metastatic GBM.

Methods and Supplementary Figure Legends

STEAM cocktail staining specifically identifies GBM cells.

Clinical association of CTC frequency