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Objective. To develop and validate a cell suspension method using Vero 76 cells for culturing Zika virus (ZIKV) based on infection of detached freshly seeded cells. Material and methods. Three different multiplicities of ZIKV infection were used to develop and compare this novel method to the standard confluent cell monolayer method. In addition, we preliminary validated the cell suspension method using well-characterized ZIKV positive and negative clinical samples. The standard confluent cell monolayer method was used as the reference method, and viral isolation was confirmed by a ZIKV-specific RT-PCR. We estimated the sensitivity, and its 95% confidence intervals, for the cell suspension method. Also, a technical comparison of the cell suspension method against the cell monolayer method was performed. Results. Our findings suggest that both the viral load and replication of ZIKV were comparable between both monolayer- and suspension-infection methods. Although both methods were suitable for culturing and isolating ZIKV, the cell suspension method was easier, cheaper, and quicker as well as a sensitive isolation technique. The cell suspension method was significantly more sensitive in detecting Zika in inconclusive cases by RT-PCR, with a fourfold increase compared to the confluent cell monolayer method. Conclusion. The cell suspension method has the potential to be an effective method for cultivating and isolating ZIKV and its application is potentially useful in both research and clinical settings. [ABSTRACT FROM AUTHOR]

Copyright of Bistua: Revista de la Facultad de Ciencias Básicas is the property of Facultad de Ciencias Basicas de la Universidad de Pamplona and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Biomédica: Revista del Instituto Nacional de Salud is the property of Instituto Nacional de Salud of Colombia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Introduction: Zika virus (ZIKV) is a flavivirus in pregnant women that can cause neurological conditions in their children. The aim of this study was to describe the neurodevelopment of 104 children with prenatal exposure to ZIKV, during the first 18 months of life. Material and methods: the patients were evaluated by neurological examination and the application of the Bayley III scale. Infection in mothers was confirmed with ZIKV NS1 IgG. Results: thirty-nine patients with neurodevelopmental delay (37.5%) and 17 patients with microcephaly (16.3%) were identified. Ocular abnormalities were detected in 25 (24%) patients, including involvement of the optic nerve, the retina, as well as the extraocular muscles. Conclusion: children of mothers with ZIKV infection during pregnancy have a high frequency of neurodevelopmental abnormalities. [ABSTRACT FROM AUTHOR]

Copyright of Comunicaciones en Estadística is the property of Universidad Santo Tomas and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Revista Latinoamericana de Hipertension is the property of Revista Latinoamericana de Hipertension and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Introduction: Zika virus is transmitted through the bite of infected mosquitoes, but it can also occur through an intrauterine infection before delivery, and the virus passes to the fetus. Objective: describe the level of impairment in performance skills and maturation age of brave boys and girls of the future program with neonatal Zika virus infection. Materials and Methods: Research is framed with a quantitative correlational approach supported by field research and non-experimental design, with a sample of 15 infants aged 3, 4 and 5 years. The data collection technique used was the Abbreviated Scale. Results: Regarding the areas evaluated with respect to maturational age, it was found that the weighting of motor skills and praxis found a correlation coefficient of 0.601 (moderate) and in the sample of performance skills of 3-year-old children. With gestational zika, a correlation of 0.853 (strong) was obtained. Discussion: Zika virus infection in boys and girls, acquired during pregnancy, strongly limits the execution skills typical of maturational age in this population. Conclusions: there is a correlation between infants with gestational zika and strong involvement in the actions or behaviors that a patient has in moving and physically interacting with activities, objects and thus performing a learned motor activity. [ABSTRACT FROM AUTHOR]

The article presents a research proposal to implement a molecular test that allows for the specific detection of the Zika virus. The importance of this test is highlighted due to the similarity of symptoms with other viruses transmitted by the same vector, such as dengue and chikungunya. The study implemented a conventional PCR test and observed virus amplification up to a concentration of 3.12 ng, without cross-reaction with the Chikungunya virus. The results allowed for the establishment of new protocols for the specific detection of the Zika virus. [Extracted from the article]

The article presents the implementation of a molecular test for the detection of the Chikungunya virus (CHIKV). It proposes implementing a regional molecular test to improve the diagnosis and management of the disease. The study shows results of simultaneous detection of the Zika virus (ZIKV) and the Chikungunya virus (CHIKV) using the conventional PCR technique. The primer concentration was optimized and a specific band for each serotype was observed starting from an RNA concentration of 3.12 ng. These results allow for the establishment of protocols for the effective detection of both viruses and can have an impact on the research and diagnosis of these diseases. [Extracted from the article]

Copyright of Gaceta Médica de Caracas is the property of Academia Nacional de Medicina and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Medicina (Buenos Aires) is the property of Medicina (Buenos Aires) and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The objective of the present study was to determine the aedic indices and establish the level of risk for the transmission of dengue, chikungunya and zika in "Barrio Ajuro", Heres municipality, Bolívar state, Venezuelan Guayana. In August 2016, we examined all possible breeding sites for Aedes aegypti found in the surroundings of the homes of said community. A total of 160 (87.4%) of the 183 houses of community were evaluated. In 154 (96.3%) of these houses, containers capable of containing water were found (possible breeding sites for A. aegypti). An estimate of 11,150 containers capable of acting as hatcheries were found, of which 3,760 contained water. A total of 88 houses with at least one container with A. aegypti larvae and/or pupae were found, with a total of 142 positive recipients. A large number and variety of containers served as hatcheries but there was a preference (p < 0.05) for abandoned rubbers tires. A total of 6,756 immature stages of Culicidae were captured (6,289 larvae and 467 pupae), of which 6,695 were identified as belonging to the genus Aedes (6,684 A. aegypti and 11 A. albopictus) and 61 to Culex. The dwelling index was 55%, containers index 3.8% and the Breteau index was 88.8%. In conclusion, considering the result of the calculated aedic indices, the community is at high risk for the transmission of dengue and by extension of chikungunya and zika viruses. [ABSTRACT FROM AUTHOR]

Since 2013, dengue, chikungunya and zika viruses have been circulating simultaneously in America. Although the primary vector of all these viruses is the mosquito, Aedes aegypti, a secondary vector that has already been reported in Venezuela is A. albopictus. In this communication, the presence of A. albopictus is reported in the northern zone of Bolívar state and some considerations are made about its importance in the epidemiology of dengue and other arboviruses. [ABSTRACT FROM AUTHOR]

Objective: To describe the epidemiological and clinical profile of microcephaly cases in Recife, Pernambuco. Method: This was a cross-sectional, quantitative study developed at a reference hospital for cases of microcephaly. Data were collected in August 2016 from the FormSUS system. All confirmed cases of microcephaly in the period from August 2015 to July 2016 were included, making up 180 cases. Data were analyzed using descriptive statistics and the chi-square and Fisher's exact tests. Results: The majority of cases occurred in October and November 2015, with 55 (30.56%) and 52 (28.89%) cases, respectively. Serology for the Zika virus was reagent for 79 (43.89%) of the infants. The most prevalent symptom during gestation was exanthema 105 (57.3%). Furthermore, 150 (83.33%) of the children were born at full term, 78 (43.33%) presented percentiles between 10 and 50 in relation to weight and gestational age and 108 (60%) had -3 standard deviations in the comparison of head circumference with gestational age, thus considered to indicate severe microcephaly. Conclusions: It is necessary to guarantee comprehensive and specialized care for these children. Epidemiological and entomological surveillance and more effective control actions in the fight against the vector are necessary. [ABSTRACT FROM AUTHOR]

The reports of cases of microcephaly in Brazil have posed a challenge for the public health, in particular, in Latin American countries such as Colombia, since despite their alleged relationship with the Zika virus, there is no information on the relation of other factors involved in the etiology of microcephaly are related with this increase in cases. There are also very few epidemiological statistics on the behavior of the etiological factors of microcephaly until the crucial year of 2015, not only in Brazil, but also in most Latin American countries. The changes in the incidence and prevalence of microcephaly are very variable due to the dissimilarities in the definitions, the development criteria and the differences among the populations with and without certified diagnostic standards and follow-up criteria for microcephaly. A general review of the microcephaly issue is made, in order to unify definitions, classification and etiology. [ABSTRACT FROM AUTHOR]

The Zika virus was discovered in 1947, and started causing epidemics in the Pacific islands in 2007. Despite the high level of infectiousness involved in these epidemics, the symptoms were very light, without any associated grave pathology. In 2014, during the epidemic in French Polynesia, the infection by the virus was associated with the Guillain Barré syndrome. However, it was towards the end of 2015, in an epidemic in Brazil, that the virus was correlated by researchers to congenital problems. This possible teratogenicity generated a worldwide scientific, juridical, moral and social debate, amplified by the celebration of the Olympic Games in Brazil. The present thesis offers an extensive exposition of the events that happened from the discovery of the virus up to the end of 2018. The first part, through a historical overview, presents the scientific facts related to the virus and the problems derived from infection, with special focus on the question of whether or not the virus causes the congenital problems. The second part analyzes the social and juridical consequences, highlighting the disconnection between the biological facts and the defense of the decriminalization of abortion in cases of infection by the Zika virus. The last part of the thesis considers, from different perspectives of moral theology, the strategies utilized by the abortionist lobby and their immorality. The contribution of the thesis consists in the systematization of the biological facts which provide the basis for a moral evaluation, and in effectuating a transdisciplinary dialogue among different areas of human knowledge. [ABSTRACT FROM AUTHOR]

Copyright of Ginecología y Obstetricia de México is the property of Federacion Mexicana de Ginecologia y Obstetricia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

A Reverse Transcription Loop-mediated Isothermal Amplification (RT-LAMP) method was developed to detect Zika. The primers were designed based on the NS5 region of 64 complete genomes. Lyophilized LAMP reagent was used. Initially, seven different arboviruses were tested and only Zika samples tested positive. Additionally, serial dilutions of one of Zika's RNA were compared using RT-LAMP and qRT-PCR, demonstrating that RT-LAMP is 1,000 times more sensitive. We also evaluated 300 serum samples with RT-LAMP comparing the results with standard qRT-PCR methods, and we obtained a 99.3% sensitivity, 100% specificity, 100% positive predictive value, and 99.3% negative predictive value. In conclusion, this method provides a low-cost, high-performance, viable, and reliable alternative for the rapid diagnosis of Zika in primary health-care facilities. [ABSTRACT FROM AUTHOR]

After the World Health Organization declared a state of emergency due to the zika virus between February and November 2016, a great deal of research has been done to analyze it. Nevertheless, this research displays two significant gaps. First, these projects are carried out in epidemiological and public health frameworks, but lacking a perspective of the social studies of science and technology. In the second place, only occasionally does attention center on the health conditions in the countries of the periphery. This article is the result of research begun in 2016 by an international team with members from Brazil, Argentina, Mexico, and the United States, financed by the National Science Foundation. The aim of the analysis is to fill that niche, centering on the specific situation in Mexico. The first focus is the public's mistrust both of the government and of the communications media. Later, it researches the citizenry's perceptions of the participation of the Ministry of Public Health and government efforts at prevention and eradicating the virus. Finally, the article presents the research conclusions, relating them to the effectiveness of government campaigns around the virus. [ABSTRACT FROM AUTHOR]

OBJECTIVES: To communicate a series of cases of Guillain-Barre syndrome and to describe the clinical characteristics related to a possible association with previous infection due to Zika virus. MATERIAL AND METHOD: A descriptive study of series of cases in which clinical, biochemical and demographic data were collected from patients with Guillain-Barre syndrome treated at the Hospital General Acapulco with a recent history of acute viral syndrome suspected of infection due to Zika virus, performed from July 24 to August 24, 2016, with conducting study of cerebrospinal fluid and ascertainment of cytological albuminous dissociation. A certainty diagnosis was established for Guillain-Barre syndrome according to the criteria of Asbury-Cornblath and Brighton. RESULTS: Ten patients with GBS and a recent history of acute viral syndrome compatible with Zika virus infection were included. The mean age was 47.8 years, 4 were male. The motor and sensitive deficits were developed at a median of 4.5 and 4.3 days, respectively after the onset of symptoms of viral infection. The complications found were ventilator-associated pneumonia, urinary tract infection and dysautonomia. CONCLUSIONS: All cases of this outbreak of Guillain-Barre syndrome had a recent history of suspected infection due to Zika virus, reinforcing the evidence for the association between Guillain-Barre syndrome and infection due to Zika virus. [ABSTRACT FROM AUTHOR]

In this article we describe the main transmission mechanisms of the Zika virus, from those already known, such as zoonotic and anthroponotic transmission of the virus, perinatal, occupational transmission, by blood products and by sexual contact. Given the possibility of person-to-person transmission, some measures of attention are proposed as the necessary studies are advanced to confirm or discard it. [ABSTRACT FROM AUTHOR]

Copyright of Biomédica: Revista del Instituto Nacional de Salud is the property of Instituto Nacional de Salud of Colombia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

With this article we review the current Zika virus (ZIKV) epidemic. We also look back over the last scientific evidence relating ZIKV to microcephaly and Guillain-Barré syndrome. Finally, we also address the challenges in terms of reproductive rights in Latin America. [ABSTRACT FROM AUTHOR]

Congenital toxoplasmosis continues to be a public health threat. Even existing guidelines, publicly known, its implementation and lack of appropriate interpretation of serological tests in pregnancy is often observed. This leds to failure in opportunities for positive and known interventions to decrease the fetal risk due to Toxoplasma gondii infection. We reported herein a case series, with variable neurological and systemic compromise (respiratory distress, hepatosplenomegaly, enterocolitis, brain calcifications, thrombocytopenia, ascites, shock), even fatal, calling for awareness about the fact that despite the Zika epidemics in 2015-2016 in Brazil, Colombia and other countries, precisely toxoplasmosis, is a differential diagnosis still prevalent in these territories, that can leds to severe consequences, with neurological disability and risk of ocular damage, even lately. Additionally, with varieties of T. gondii with more aggressive patterns in Latin America, which make worse those cases, including also a higher risk of death. [ABSTRACT FROM AUTHOR]

L'effet tératogène du virus Zika (VZIK) a récemment été confirmé. Il faut envisager le diagnostic de syndrome associé à l'infection congénitale à VZIK chez les enfants qui présentent une microcéphalie inexpliquée, des calcifications intracrâniennes, une ventriculomégalie ou des anomalies structurelles majeures du système nerveux central. La prise en charge est en constante évolution, mais des suggestions sont proposées pour les enfants chez qui les constatations évoquent une infection congénitale et ceux nés d'une mère susceptible d'avoir été exposée au virus pendant sa grossesse. [ABSTRACT FROM AUTHOR]

Taking into account that nowadays emergencies and reemergencies of infectious diseases exist, fundamentally in tropical areas, it is necessary to advice health professionals and health authorities, about the threat that represents the Zika virus. To such effects an exhaustive literature review was carried out where some aspects of interest are analyzed, such as: vectors and pouches, transmission ways, clinical features, differential diagnosis and treatment, among others, which will help to increase the knowledge level in this respect and be prepared to face any epidemic disease of this nature. [ABSTRACT FROM AUTHOR]

In the present work an exposition of the most frequent vectors of the arboviruses is made highlighting the ones of the genus Aedes, of these in each geographic distribution there are specific subgeneros, in Africa emphasizes A. africanus, A. opok and A. vitatus like those in charge of The propagation of arboviruses. In Asia and Oceania, A. hensilii and A. polynesiensis stand out, and A. albopictus, in addition to A. koreicus and A. japonicus, the latter also responsible for dissemination in North America. It is mentioned in the wave that struck to South America the greater importance of other genera like Culex, emphasizing C. pipiens, C. perfuscus, C. quinquefasciatus and C. nigripalpus; Also genera like Anopheles emphasizing A. coustani, A. albimanus and A. pseudopunctipennis (Central America and Mexico). Therefore the importance of other vectors other than Aedes as the participants in the American wave is pointed out. [ABSTRACT FROM AUTHOR]

El artículo discurre sobre el desarrollo de vacunas experimentales contra el Zika. Los autores comentan sobre la transmisión del virus por el mosquito hembra Aedes aegyptii y Aedes albopictus, y examinan la propagación del trastorno por Brasil y otros países sudamericanas y caribeñas en 2015. También se analiza la epidemiología, el ciclo de vida viral y la genoma del virus.

This article summarizes the recommendations issued by the agencies and national and international health authorities with regard to the recent outbreaks of the virus Zika, currently present in more than 31 countries and territories, mainly from central and South America. In October 2015 the first indigenous case of infection was identified by virus Zika in Mexico and in the absence of a vaccine or specific treatment, the promotion of health is essential for risk communication and community mobilization, especially against the vector. The morbidity and mortality of the disease up to now known and recommended for its prevention actions, arising from actions by authorities and bodies in cases like this, of emerging viral diseases, are fundamental to contribute to timely detection and reduced irrigation resulting in a threat to the entire population and certainly one threat to patients with chronic diseases, because it should be considered that in little-known ailments and complications, infections can be more aggressive. More even when studied neurological associations such as microcephaly and Guillain-Barré Syndrome. It should be extra alert in the presence of other viral infections present in Mexico, Chikungunya fever, Dengue and hemorrhagic Dengue fever, transmitted by mosquitoes. [ABSTRACT FROM AUTHOR]

In this paper the relationship between the incidence of microcephaly and various arboviruses in current boom is exposed. It stands out that in the infection by the dengue virus, there have been no cases of microcephaly, however in the case series of chikungunya infection, incidence of cases with this affectation was reported, highlighting a cohort called CHIMERE; also the highest incidence of microcephaly associated with the mentioned Zika virus, which has been widely studied. All this, due to the neurotropism of these arboviruses. [ABSTRACT FROM AUTHOR]

Copyright of Revista Medicina is the property of Revista Medicina and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Dengue virus three, Chikunguya and Zika have entered the national territory through the south of the country. Cases and outbreaks of yellow fever have now been identified in the Americas where it threatens to expand. Although Mexico has a robust epidemiological surveillance system for vector-borne diseases, our country must be alert in case of its possible introduction into the national territory. This paper presents theoretical assumptions based on factual data on the behavior of yellow fever in the Americas, as well as reflections on the epidemiological surveillance of vector-borne diseases [ABSTRACT FROM AUTHOR]

En Colombia por cada 100.000 nacidos vivos 76 madres pierden la vida a causa del embarazo de alto riesgo (ARO). En la ciudad de Barranquilla existen un gran conjunto de factores de riesgo que pueden influir en la alteración de las semanas de embarazo e incluso en la mala formación del feto y síndromes neurológicos con antecedente de enfermedad compatible con infección por virus Zika (ZIKV). El objetivo dese estudio es saber la frecuencia de mujeres embarazadas con ARO por el ZIKV en el período de octubre de 2017- mayo de 2018 en el Hospital Materno Infantil del municipio de Soledad. Durante ese período, 2547 estuvieron clasificadas con ARO, de las cuales 504 (19,78%) por el ZIKV. Hasta la semana epidemiológica 34 de 2018 se han notificado 16 casos sospechosos de síndromes neurológicos (Síndrome Guillain-Barre, Encefalitis viral no especificada y otras enfermedades degenerativas de sistema nervioso) y con el aumento de casos de ZIKV en mujeres embarazadas, esa estadística debe subir en los próximos meses. [ABSTRACT FROM AUTHOR]

En Colombia por cada 100.000 nacidos vivos 76 madres pierden la vida a causa del embarazo de alto riesgo. En la ciudad de Barranquilla existen un gran conjunto de factores de riesgo que pueden influir en la alteración de las semanas de embarazo e incluso en la mala formación del feto, como por ejemplo la hipertensión gestacional y preclamsia, sífilis gestacional, la no utilización de malla para protección contra vectores transmisores de enfermedades infecciosas como el Aedes aegypti principalmente, ya que este puede ser trasmisor de enfermedades tales como el Chikungunya, Dengue y Zika que puede provocar en el feto microcefalia. El objetivo dese estudio es Identificar los factores de riesgo que predisponen a las mujeres embarazadas en el Hospital Materno Infantil del municipio de Soledad / Atlántico en los meses prenatales de julio de 2016 y agosto 2017. Algunos factores físicos que predisponen a un alto riesgo obstétrico son los extremos de edad (29.6), retraso en el crecimiento (8.2%), un índice de masa corporal fuera de los parámetros normales (52%) y controles retrasados prenatal (73%), el anomalías en el cuello uterino (2%) y anemia (55%). [ABSTRACT FROM AUTHOR]

We report a case of intrauterine infection by Toxoplasma gondii, Chikungunya and Zika viruses in a Colombian woman from the southern part of the country. The patient attended prenatal care in the second trimester of her pregnancy and she informed that in the first trimester she had presented with clinical symptoms compatible with Zika virus infection. Amniotic fluid PCR assays showed infection by T. gondii, chikungunya and Zika viruses. Diagnostic imaging showed fetal malformation of the central nervous system. At 29 weeks of gestation, pregnancy was terminated medically. [ABSTRACT FROM AUTHOR]

Introduction: Zika virus (ZIKV) is an enveloped flavivirus transmitted to humans mainly by Aedes aegypti. ZIKV infection has been associated with high neurotropism and neuropathic effects such as the Guillain-Barré syndrome in adults, and fetal and postnatal microcephaly and the congenital Zika virus syndrome similar to that produced by rubella virus (VR). Objective: To compare Zika virus membrane protein E (E-ZIKV) and rubella virus membrane protein E1 (E1-RV), and to propose possible implications for neurotropism and nervous system disorders associated with ZIKV infections. Materials and methods: The amino acid sequence of E-ZIKV protein (PDB: 5iZ7) was aligned to that of rubella virus glycoprotein E1 (PDB: 4ADG). The secondary structure elements were determined using the programs Vector NTI Advance®, DSSP, and POSA, and integrated data management tools (AlignX®). One of the main comparison and alignment criteria was the allocation of structurally equivalent residues with more than 70% identity. Results: E-ZIKV structural organization (PDB: 5iZ7) was similar to that of E1-RV (PDB: 4ADG) (70%-80% identity), and it was consistent with relevant structural features of viral membrane class II fusion glycoproteins. E-ZIKV and E1-RV exhibited highly conserved fusion structural elements at the distal region of domain II, which has been associated with the RV myelin oligodendrocyte glycoprotein and Axl cell receptors in ZIKV and other flaviviruses. Conclusion: The comparison of E-ZIKV and E1-RV proteins constitutes an essential step towards the definition of ZIKV neurotropism and pathogenesis molecular determinants, and for the adoption of diagnosis, prevention and treatment strategies against neurological complications induced by ZIKV infection. [ABSTRACT FROM AUTHOR]