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The objective was to detect QTL of agronomic interest in the segregating generations F2 and backcrosses of a tomato (Solanum lycopersicum L.) second cycle hybrid (SCH) obtained between two recombinant inbred lines derived from the interspecific cross S. lycopersicum cv. Caimanta x S. pimpinellifolium LA 722. Molecular characterization was achieved by AFLP markers and agronomic traits under study were fruit weight, diameter, height, soluble solids content, acidity, color, pH, shape, firmness and postharvest life. Single point analysis was applied to detect QTL, assessing the association of AFLP polymorphic bands and quantitative traits with significant genetic variance. Amplifications with six primers combinations, selected because of detecting high polymorphism percentage, 221 AFLP bands were obtained and 135 (61.1%) of them were polymorphic in the tomato populations analyzed. In the F2 population, 29 polymorphic fragments adjusted to the expected mendelian segregation and a total of 28 QTL were detected for all evaluated traits. In the backcross population, 15 polymorphic fragments adjusted to the expected mendelian proportion 1:1 (12 of these fragments showed a de novo pattern) and a total of 13 QTL were identified for the traits soluble solids content, height, weight and diameter. AFLP allowed to identify QTL of agronomic interest in the segregating generations of a tomato SCH. [ABSTRACT FROM AUTHOR]

The genetic diversity among 30 Cuban strains of Ralstonia solanacearum isolated from potato and tomato and belonging to biovars 1 and 2, was assessed by the repetitive sequence based polymerase chain reaction (rep-PCR) method with ERIC primer sets. The method defined 11 genotypes from all the strains. The primary groupings obtained by cluster analysis corresponded with the strain biovar, while the subdivisions found within each group were related to the host from which the strains were obtained. Potato isolates showed a 37.2% similarity and the presence of specific variants within each biovar for each geographic region. Isolates from tomato showed a similarity of 71.3 % with the cross presence of the same genotype in different geographic regions and the incidence of different genetic variants in the same region. The Principal Component Analysis explained 81.32 % of isolate variability, which corroborated the groupings according to the host and biovar and allowed to establish relationships based on the geographic location. The differential distribution of the genetic diversity among Cuban R. solanacearum strains from potato and tomato crops may be useful information to establish specific measures to control the bacterial wilt disease in Cuba. [ABSTRACT FROM AUTHOR]

Copyright of Revista Mexicana de Biodiversidad is the property of Universidad Nacional Autonoma de Mexico, Instituto de Biologia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

With the objective of continuing increasing the genetic diversity of the species Lycopersicon lycopersicum L. in hands of those producing of Topes of Collantes and to contribute to that the same ones introduce varieties well adapted to the conditions of their properties, they were carried out investigations in areas of the property of a producer belonging to CCS "Lucas Castellano". He/she was carried out a comparative test of yield and a fair of Participatory Fitomejoramiento. The results of the comparative test were evaluated by means of an Analysis of Variance of double classification and the test of Duncan for the comparison of the stockings. The results of the fair were analyzed by the number of times that each variety was selected, being also determined the influence of the gender. The Varieties FM, R2, Variety 38, Variety 1 and R2 Masal reached the biggest values in the comparative test. It is recommended to continue the generalization work in the commercial production of the varieties FM, R2, 38, 1 and R2 Masal. [ABSTRACT FROM AUTHOR]

Copyright of Revista Colombiana de Biotecnología is the property of Universidad Nacional de Colombia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Flower anthesis is a crucial stage for the ovary's fate because endogenous stimuli -pollination or high hormonal level- or exogenous ones -hormonal application- induce the fruit set. In the absence of these stimuli, the ovary stops growing and starts the senescence process. It has previously observed that, in unpollinated ovaries, the senescence goes together with the increase in the activity/expression of certain thiolproteases, parameters that decrease during the early stage of fruit development. The objective was to analyze the location of proteases and anatomic changes associated with the fruit set or ovary senescence processes that take place during the early stage of the tomato fruit development obtained by natural pollination or by treatment with GA3. During the period in which the unpollinated ovaries keep their sensitivity to the hormone application, an accumulation of polypeptides related antigenically with papain thiolprotease was observed, mainly in the epidermis of the pericarp, showing no signs of alterations in the tissue integrity. Fruits of 8 days of development obtained with GA3, compared to the pollinated ones, showed an increase in the thickness of the pericarp related mainly with a higher enlargement of the mesocarp cells. [ABSTRACT FROM AUTHOR]

Begomoviruses are plant emergent viruses. Due to the persistent TYLCV infections in tomato fields, the losses caused by theses viruses in the worldwide agriculture and the high variability reported for most of their members, the objective of this work was to characterize the genetic variability of the natural population of TYLCV in Cuba. Twenty one isolates of TYLCV, collected from several years and regions of the country, were amplified and sequenced using specific primers that amplify the intergenic region (IR) and the 5′ end of c1 gene or replication protein (rep). The sequences obtained were analyzed by bioinformatic softwares to determine the phylogenetic relationship with other TYLCV isolates reported in Genkbank, and predict possible secondary structures. Results evidenced the presence of an only one TYLCV population in the country, where all the isolates showed between 97-99% identity of their nucleotide sequences with that TYLCV previously isolated in Cuba. The presence of three different secondary structures with high thermodynamic stability was determined, being the predominant structure similar to that of TYLCV-Egypt and different from that of TYLCV-Isr. These results provide new elements for the characterization of the genetic diversity and control of TYLCV, and could explain the possible causes of the wide dissemination of the virus observed in the Cuban tomato fields. [ABSTRACT FROM AUTHOR]

The article offers information on the transformation of green tomato or the physalis ixocarpa var. rendidora genome plants. It examines a rapid Agrobacterium tumefaciens-mediated transformation system of green tomato. Leaf explants were inoculated with A. tumefaciens strain EHA-R 105 harboring the vector pCAMBIA. Transgenic plants were regenerated to leaf explants. The process revealed that one line of four transgenic lines was morphologically normal. The four transgenic lines showed different levels of papain inhibition.

Tomato Yellow Leaf Curl disease is one of the most important problems affecting tomato cultivation in most part of the Cuban growing areas and in other countries. The casual agent is the TomatoYellow Leaf Curl Virus (TYLCV). At the moment several genes related with the resistance have been described. The genes Ty-1, Ty-2 and Ty-3 are those that more they have been used in the plant breeding programs of the species. In Cuba, Vyta is only variety that had Ty-1 gene and other accessions carrying the Ty-2 gene. The amplification of the gene Ty-3 was carried out for PCR and were used the specific primers FLUW-25R and FLUW-25F. In this work the presence of the band related with the resistant pattern of the gene Ty-3 was identified in one that six characterized accessions. [ABSTRACT FROM AUTHOR]

DOMI is a tomato mutant obtained at the National Institute of Agricultural Sciences (INCA) after irradiating seeds from Amalia variety with 500 Gy gamma rays. This variety has a high productive potential and an appropriate quality for fresh consumption and industrial purposes. [ABSTRACT FROM AUTHOR]