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Use of mesenchymal cells has emerged as an important part in regenerative medicine with positive effects in the recovery of damaged or lost tissues. Chronic ulcers include skin lesions that affect a large part of the population with diabetes mellitus, (for example, 9.14 develop diabetic foot according to ENSANUT 2016) and represent considerable economic expenses, due to poor quality of life in patients. The conventional treatment that is carried out in these cases is long term, and the generated beneficial effects that are presented are limited, since the injuries recur due to not having a specific and regenerative treatment, only palliative. That is why in this work they were obtained and used mesenchymal stem cells derived from Wharton's jelly (MSC-WJ) under the criteria proposed by the International Society for Cell Therapy. The treatment was applied in two patients with chronic ulcers phlebostatic of different medical conditions and short-term monitoring, so it was a limited study. The results showed that MSC-WJ induced skin reconstruction by tissue formation granulation, in addition to decreasing the chronic inflammatory process in the area of damage, which favored the index of wound healing. Therefore, the use of MSC-WJ in chronic phlebostatic ulcers is proposed as a treatment in the regeneration of the tissue structure with short-term results. [ABSTRACT FROM AUTHOR]

Mesenchymal stem cells (MSC) represent a heterogeneous population with the capacity to self-renew and differentiate into different cell types. At the middle of the last century these cells initially were described in bone marrow (BM), thence this tissue has become the gold standard for obtaining and characterization of MSC. It is known that these cells are housed in specific areas called niches distributed throughout all body, where they contribute to tissue regeneration processes of self-tissue were they are located. However, finding an alternative source of CTM with the same characteristics that have showed in MO, but its obtention no represent a risk since the donor is essential to their use for therapeutic purposes. In this study we isolated mesenchymal stem cells from bone marrow, adipose tissue and Wharton's jelly and they were compared in their characteristics in according to the standards of the International Society for Cellular Therapy (ISCT). The results showed that the morphology as well as adipogenic and osteogenic differentiation and also the expression of surface antigens (CD90, CD73, and CD105) from all tissues accomplished the standards, although Wharton's jelly represented the best option. [ABSTRACT FROM AUTHOR]

Copyright of Revista Mexicana de Ingeniería Biomédica is the property of Sociedad Mexicana de Ingenieria Biomedica, A.C. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Revista Mexicana de Ingeniería Biomédica is the property of Sociedad Mexicana de Ingenieria Biomedica, A.C. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Cirugía y Cirujanos is the property of Publicidad Permanyer SLU and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Biomédica: Revista del Instituto Nacional de Salud is the property of Instituto Nacional de Salud of Colombia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Biomédica: Revista del Instituto Nacional de Salud is the property of Instituto Nacional de Salud of Colombia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Biomédica: Revista del Instituto Nacional de Salud is the property of Instituto Nacional de Salud of Colombia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Introduction: Every year about 5,000 are reported a10, 000 patients with congenital or acquired esophageal requiring surgical treatment, unlike the rest of the gastrointestinal tract is not possible to use autologous tissue for reconstruction because of its limited size and its delicate vascularity, which are used by complex procedures such as colon interposition, a field that has been growing and could provide a solution to these problems is tissue engineering. The purpose of this work involves the construction of a esophageal prosthesis from autologous cells implanted on a collagen matrix and test its usefulness in an animal model. Material and Methods: We took a biopsy of healthy esophageal tissue at the neck of healthy rabbits, which were processed to desegregate its cellular components in epithelial and muscle cell viability was verified by MTT method and their presence by electron microscopy scanning. The prosthesis was implanted in a second surgery to create a defect in "patch" in the native esophagus, further studies of endoscopy, esophagogram and necropsy in animals. Results: After the biopsy epithelial and muscle cells were isolated and multiplied sown and harvested in Petri dishes and seeded at a concentration of 1x! 07 cel/cm2 in intestinal submucosa prostheses. Subsequently, the prostheses were implanted in four healthy rabbits N = 5, all rabbits subsequently underwent esophagogram and endoscopy studies, observing proper integration of the now called neoesófago, with adequate passage of contrast medium showed no leakage or stenosis, all animals remained alive, feeding by mouth without complications so far from being slaughtered except one who died for reasons unconnected with the study. In histopathological studies, there is an almost normal architecture neoesófago presence of mucosa and muscle layer proper. Conclusion: In this study we establish the conditions, general characterization for the isolation and multiplication of epithelial cells and muscle tissue healthy esophageal also created an esophageal prosthesis, which could be implanted in animals to which a defect was created 50 % of the light of it with proper integration of the now called neoesófago. All animals remained alive feeding by mouth without complications, which lays the basis for developing an esophageal prosthesis in the future which can be used in the treatment of children with congenital or acquired esophageal lesions. [ABSTRACT FROM AUTHOR]