Creación exitosa de Neoesófago mediante Ingeniería de Tejidos en un Modelo Animal.

Introduction: Every year about 5,000 are reported a10, 000 patients with congenital or acquired esophageal requiring surgical treatment, unlike the rest of the gastrointestinal tract is not possible to use autologous tissue for reconstruction because of its limited size and its delicate vascularity, which are used by complex procedures such as colon interposition, a field that has been growing and could provide a solution to these problems is tissue engineering. The purpose of this work involves the construction of a esophageal prosthesis from autologous cells implanted on a collagen matrix and test its usefulness in an animal model. Material and Methods: We took a biopsy of healthy esophageal tissue at the neck of healthy rabbits, which were processed to desegregate its cellular components in epithelial and muscle cell viability was verified by MTT method and their presence by electron microscopy scanning. The prosthesis was implanted in a second surgery to create a defect in "patch" in the native esophagus, further studies of endoscopy, esophagogram and necropsy in animals. Results: After the biopsy epithelial and muscle cells were isolated and multiplied sown and harvested in Petri dishes and seeded at a concentration of 1x! 07 cel/cm2 in intestinal submucosa prostheses. Subsequently, the prostheses were implanted in four healthy rabbits N = 5, all rabbits subsequently underwent esophagogram and endoscopy studies, observing proper integration of the now called neoesófago, with adequate passage of contrast medium showed no leakage or stenosis, all animals remained alive, feeding by mouth without complications so far from being slaughtered except one who died for reasons unconnected with the study. In histopathological studies, there is an almost normal architecture neoesófago presence of mucosa and muscle layer proper. Conclusion: In this study we establish the conditions, general characterization for the isolation and multiplication of epithelial cells and muscle tissue healthy esophageal also created an esophageal prosthesis, which could be implanted in animals to which a defect was created 50 % of the light of it with proper integration of the now called neoesófago. All animals remained alive feeding by mouth without complications, which lays the basis for developing an esophageal prosthesis in the future which can be used in the treatment of children with congenital or acquired esophageal lesions. [ABSTRACT FROM AUTHOR]