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Objective. Determine cell viability, Hsp70 protein expression, degradation of genetic material and cell death by apoptosis in Katahdin, Dorper ovine and human leukocytes irradiated in vitro with UVA radiation. Material and methods. Leukocytes, which were exposed in vitro to UVA radiation, were used to evaluate cell viability, DNA fragmentation, Hsp70 protein expression and morphological alterations related to apoptosis of each model. Results. A drop in the viability of leukocytes exposed to UVA radiation was found, with ovine being the most affected. An increase in the expression of Hsp70 was observed in human, Katahdin and Dorper leukocytes. A progressive increase in DNA fragmentation was found, as well as an increase in morphological alterations related to apoptosis as the time of exposure to UVA radiation increased, with the Dorper model being the most affected. Conclusions. UVA radiation generates stress in ovine and human leukocytes, observing that humans leukocytes are more resistant, while ovines are more susceptible, mainly the Dorper genotype. [ABSTRACT FROM AUTHOR]

Copyright of Revista Pediatría de Atención Primaria is the property of LUA Ediciones 3.0 S.L. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Ginecología y Obstetricia de México is the property of Federacion Mexicana de Ginecologia y Obstetricia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of QhaliKay: Revista de Ciencias de la Salud is the property of QhaliKay Revista de Ciencias de la Salud and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Introduction: Chronic obstructive pulmonary disease (COPD) is the third leading cause of death, and COPD exacerbation worsens the prognosis. Eosinophilic airway inflammation is a COPD phenotype that causes COPD exacerbation and is correlated with peripheral blood eosinophil count. We analyzed real-world data of COPD patients to assess the risk factors of COPD exacerbation focusing on blood eosinophils. Materials and methods: Patients with COPD who visited our hospital between January 1, 2018, and December 31, 2018, were recruited, and their background information, spirometry data, laboratory test results, and moderate-to-severe exacerbation events during the one-year follow-up period were collected from the electronic medical records and analyzed. The COPD exacerbation risk factors were assessed using univariate and multivariate logistic regression analyses. Results: Twenty-two of 271 (8.1%) patients experienced moderate-to-severe exacerbation. Patients with exacerbation showed worse pulmonary function, and we found that a high blood eosinophil count (≥350 cells/μL; p=0.014), low % FEV1 (<50%; p=0.002), increase in white blood cell (≥9000 cells/μL; p=0.039), and use of home oxygen therapy (p=0.005) were risk factors for future exacerbations. We also found a strong correlation between eosinophil count cut-offs and exacerbation risk (r = 0.89, p < 0.001). On the other hand, there was no relation between exacerbation risk and inhalation therapy for COPD. [ABSTRACT FROM AUTHOR]

Copyright of Agroindustrial Science is the property of Agroindustrial Science and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Revista Habanera de Ciencias Médicas is the property of Universidad de Ciencias Medicas de La Habana and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Mastitis is a frecuently disease of dairy cattle that leads to a decrease in production and an increase in health costs. This disease can be evaluated through the number of white blood cells per milliliter of milk, which is known as somatic cell count (CCS). Alleles of Bovine Leukocyte Antigen (BoLA) genes were associated with resistance and susceptibility to infectious diseases. The objective of this work was to study the polymorphism of the class II molecules of BoLA-DQA1 and their association with resistance/susceptibility to mastitis measured through the CCS in milk in Holstein cattle from the province of La Pampa. The population was divided into: 1) case group with high CCS and presence of mastitis (≥ 250,000 cel/ml, susceptible) and 2) control group with low CCS (<250,000 cel/ml, resistant). BoLA-DQA1 polymorphisms were genotyped in sixty animals by PCR-RFLP and PCR-SBT. Woolf-Haldane's Fisher and Odds Ratio (OR) exact test were used to study the association between CCS and allelic variants. 16 alleles of the BoLA-DQA1gene were detected and the BoLA-DQA1*0101 allele showed an OR of 4 (p = 0.58). This finding highlights the importance of BoLA-DQA1 alleles for selection of resistant animals to infectious diseases. [ABSTRACT FROM AUTHOR]

The knowledge of being able to determine skeletal maturation and missing growth potential is an essential element when planning orthodontic treatment. The study of craniofacial growth and development is a complex and essential process for the diagnosis and resolution of malocclusions. Craniofacial growth and development occurs by the process of growth and ossification of the hyaline cartilage, thus promoting the theory of the servosystem, which is a system with feedback, of constant renewal, which generates a new response. From this point of view, Petrovic considers involving orthopedic appliances, where he explained that the mandibular condyle can respond to mechanical stimuli causing stress at the level of anatomical structures due to orthodontic forces through the interaction of blood circulation and cells white cells that as a result form bone remodeling, which could be inhibitory or synergistic. The objective of this review is to summarize recent concepts on craniofacial growth, focused on the theory of Alexandre Petrovic and his collaborators, with the development of general scientific knowledge and inspire the creation of a new, more integrated multidisciplinary approach based on the person, through bases of MEDLINE, EMBASE, Pubmed, and Google Scholar data, which were evaluated, and synthesized, to be part of the next literature review. [ABSTRACT FROM AUTHOR]

Objective. Analyze the leukogram of meat-type quail inoculated in ovo with conjugated linoleic acid (CLA) and lauric acid (LA) while exposed to a sanitary challenge. Material and methods. The drinking water was placed seven days before the arrival of the quails and it was only refilled throughout the experiment. The treatments were applied on 480 fertile eggs in the 7th day of incubation: Negative control: not perforated; positive control: injected with the diluent (corn oil; CO); CLA120: 120 mg CLA /CO; CLA240: 240 mg CLA /CO; LA60: 60 mg LA /CO and LA90: 90 mg LA /CO. A completely randomized design with four replications was used. Total leukocytes and the differential leukocyte count were evaluated at 21 and 36 days of age. Results. No statistically effects (p>0.05) of any inoculations were observed in the parameters evaluated. We observed an average of 10927.1±2933.6 cell/µl, 61.6 ± 7.31%, 32.0 ± 7.13% and 0.57 ± 0.21 for leukocyte count, lymphocyte, heterophile and heterophile/lymphocyte ratio, respectively, at 21 days. At 36 days, we observed an overall average of 13291.7 ± 3559.0 cell/µl, 65.0 ± 9.29%, 29.3 ± 9.93% and 0.50 ± 0.24, for those same variables, respectively. Conclusions. The in ovo inoculation levels of CLA and LA did not affect the leucogram of meat-type quails raised in sanitary challenge. [ABSTRACT FROM AUTHOR]

Background: Efficient microorganisms offer a broad application spectrum in crop and animal production. Aim: to evaluate the effect of a biopreparation containing autochthonous efficient microorganisms in Guantanamo (MEAG), Cuba, on the bioproductive and hematologic parameters of swine herds. Methods: A total of 96 animals (Duroc/CC21) weighing 1.55±0.27 kg were included in the experiment since birth, and distributed in four groups with 24 repetitions, following a completely randomized design. The treatments consisted in the control and addition of the biopreparation, in 2.0, 10.0, and 20.0 mL.kg-1 doses live weight/day, orally. The experiment lasted 33 days, and the final weight, weight gain, mean daily gain, feed conversion, mortality, morbidity, hemoglobin, hematocrit, total leucocytes, eosinophils, lymphocytes, and monocytes were analyzed. A simple analysis of variance was performed, and the mean differences were detected using the multiple range test. Results: The results were better (p<0.05) in the 2.0 mL dose of the MEAG/kg live weight biopreparation associated with final live weight and weight gain (0.59; 0.70, and 0.66 kg), and mean daily gain; whereas it was lower in feed conversion in the control and treatments two and three. In morbidity alone, differences between the control group and treatment three were observed, though no differences were detected between the two remaining groups. The hematological values rose with larger doses, but within the normal range. Conclusions: The utilization of MEAG as a feed additive improves the bioproductive and hematological parameters of swine herds. [ABSTRACT FROM AUTHOR]

The ex vivo cultures of leukocytes implementation in laboratories have made it possible to evaluate the production of pro-inflammatory cytokines in different research trials on drugs or other molecules, besides, to evaluate the expression of specific cytokines, this is the reason it should be improved and studied the conditions of implementation of these cultures to make them more viable and to avoid their activation so that in this way they can be uses to carry out at studies that that requiere the application of this technique as a perfect basis. [ABSTRACT FROM AUTHOR]

Grassland bird populations have decreased significantly due to environmental contaminants, evaluating their health status through biological indicators is a way to prevent population decline. The aim of the contribution was to examine the use of hematic biomarkers (Polychromatic erythrocytes; PCE, polychromatic erythrocytes with micronucleus; PCEMN, polychromatic erythrocytes with nuclear extensions; PCEPN, erythrocytes micronucleated; MNE, erythrocytes with nuclear extensions; PNE, leukocyte count and ratio H/L) and the possible exposure to xenobiotic substances of the grasshopper sparrow (Ammodramus savannarum) and Baird's sparrow (Centronyx bairdii). During the winter season of 2020 in Cuchillas de la Zarca, Durango, 57 specimens were collected (34 A. savannarum and 23 C. bairdii), the basal frequencies of the hematic biomarkers were obtained, whose values did not constitute evidence that the birds had been exposed to polluting substances such as agrochemicals, metals and metalloids. The basal frequencies of the blood biomarkers obtained in this study were similar to those reported in other apparently healthy bird species. The observed frequency of PCE and MNE suggests the possibility of using these bird species as biomonitor candidates when exposure to cytotoxic or genotoxic compounds is suspected. [ABSTRACT FROM AUTHOR]

This article presents a determination of haematological parameters in Pelibuey lambs in the humid tropic of Mexico. A total of 80 blood samples were collected from clinically healthy male lambs of 3-4 months of age in with a live weight of 20.03 ± 7.50. The resulting data set was analyzed using Gaussian distribution and descriptive statistics. Confidence intervals of 95 % were established. Haematological parameters in the Pelibuey lambs were haematocrit: 36.62 ± 3.80 %; red blood cell: 11.71 ± 1.13 x106/µL; mean corpuscular volume: 31.28 ± 1.92 fl; haemoglobin: 12.01 ± 1.80 g/dL; mean corpuscular haemoglobin: 10.26 ± 1.30 pg; mean corpuscular haemoglobin concentration: 32.95 ± 4.46 g/dL; white blood cell: 11.55 ± 5.52 ×10³/µL; and platelets: 509.98 ± 141.61 ×10³/µL. Most haematological parameters in the Pelibuey lambs were similar to those reported for male lambs from other breeds in the humid tropic, although the red blood cells were upper. The mean corpuscular haemoglobin and mean corpuscular haemoglobin concentration were slightly lower. The present study could help in realistic evaluation of the management practices and diagnosis of health condition of Pelibuey male lambs under humid tropic conditions. [ABSTRACT FROM AUTHOR]

Copyright of Opuntia Brava is the property of Universidad de Ciencias Pedagogicas de Las Tunas, Centro de Documentacion e Informacion Pedagogica and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Selenium decreases cellular damage caused by oxidative stress and improves the organism physiological response. Leukocytes were evaluated during embryonic development in ewes with consumption of organic selenium. A total of 27 local ewes at the municipality of San Andrés Chiautla, Mexico State, with an average age of 2.5 years, were randomly assigned in two groups, 11 in control group (without Selenium, S-Se) and 16 with oral selenium (C-Se; selenium-enriched yeast) addition at dose of 0.3 ppm, for a period of seven days before Artificial Insemination (AI) consuming a diet based on alfalfa and corn stubble. White cell differential counting was performed on blood smears. Samples were taken before, during and after selenium treatment. Data were analyzed by mixed model, showing significant differences by treatment and time (p ≤ 0.05) in neutrophils; day 9 of embryonic development (5.91 ± 0.89 y 2.11 ± 0.46; S-Se y C-Se, respectively) and basophils amount was at day -3 (1.85 ± 0.31 y 1.05 ± 0.23). Regarding lymphocytes and eosinophils, they showed a difference in time (p ≤ 0.05) but not by treatment (p ≥ 0.05). It is concluded that selenium decreases the number of neutrophils and basophils; after blastocyst formation and four days after starting organic selenium treatment. [ABSTRACT FROM AUTHOR]

When a person is bitten by a venomous snake he orshe needs to get medical attention as quick as possible. Damage caused by the poison from certain species can be irreversible, mostly in cases where necrosis is found. In this cases it is possible the patient can lose the affected extremity since the venomis rapidly propagated in tissue. Currently, new alternatives are being researched to control or delay the effect of venom. This could allow time enough for the person to be taken care of with the corresponding serum. Poisoning by snake bite is very common in Mexico and it represents a serious public health problem. The most common case of this is by the Bothrops asper species mainly in the southeast part of the country. The usual treatment against this is the administration of polyvalent serum which is considered as the most effective treatment. However, the use of extract of the Randia aculeata plant has been proven to be also effective as a anti-ophidian treatment against the venom making the patient go through a faster recovery thus, avoiding medical complications common in this kind of patients. Anintoxication of male mice of the CD1 strain was made. We had 4 groups: a) Control: administered with saline solution (s.s), b) Intoxicated with B. asper venom, c) b) Intoxicated with B. asper venom + faboterapico, b) Intoxicated with B. asper venom + faboterapico + ethanolic extract of R. aculeata. After 24 hours lungs were extracted and, after an histological technique, were observed by microscope. Micro photographs were taken and histological damage was evaluated. Relative lung weight was also obtained. The venom provoked necrosis, edema butal so several leukocytes were found which were protected by the faboterapico. R. aculeata helps to get this protective effect. [ABSTRACT FROM AUTHOR]

Copyright of Ginecología y Obstetricia de México is the property of Federacion Mexicana de Ginecologia y Obstetricia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Introduction. Mean platelet volume (MPV) has been proposed as a diagnostic, prognostic and response biomarker for treatment in various inflammatory diseases, including acute appendicitis (AA), but its results are variable and confusing. This work was conducted to evaluate whether MPV can be used as a diagnostic marker in patients with and without AA, as well as between patients with uncomplicated AA (AAnC) and complicated AA (AAC). Material and Methods. In a case-control study, men and women. 18 years of age were included. Patients with acute intra-operative pathology different from AA and if post-operative histopathology did not demonstrate its presence were excluded from the final analysis. The group with AA was formed of 107 patients and the group without AA with 217 patients. The cases of AA were categorized as UAA (n = 75) and CAA (n = 32). In all groups the MPV, platelet count, leukocytes and neutrophils were determined. Results. There was no statistically significant difference in MPV (p = 0.249) between patients with AA and without AA. The MPV was higher in the group with CAA than in the group with UAA (p = 0.038). In the analysis of the receiver operating characteristic curve, the area under the curve for the MPV and the presence of CAA was 0.66 (p = 0.007, 95% CI: 0.55 - 0.77). The MPV cut-point = 10.8 fL in predicting CAA combined a sensitivity of 68.8% with a specificity of 57.3%, a positive predictive value of 22.81% and a negative predictive value of 62%; the positive likelihood ratio was 0.69 and the negative likelihood ratio was 1.44. Discussion. The MPV has no clinical utility as a diagnostic marker in cases of UAA and CAA. [ABSTRACT FROM AUTHOR]

Alfalfa, Medicago sativa L, is a highly organic legume, easy to grow and commercialize. In Peru, it is cultivated as fodder for animals. Objectives: To determine the effect of alfalfa extract consumption on the white blood cell count in albino mouse. Methods: Experimental study. carried out in Bioterio of the Facultad de Farmacia y Bioquímica of Universidad Nacional Mayor de San Marcos. Twenty albino mice of the balb / c strain, male, of average weight 23.2 ± 32.8 g were used. Two groups were formed: experimental and control, each of ten mice. The experimental group was administered, orally by cannulation alfalfa extract, in a dose of 250 mg / kg, volume of 2 ml, and the control group, also, orally by cannulation, placebo, volume of 2 ml, every five hours, for a week, alternating with standard nutrients. Leukocyte count was performed. Results: Within the conditioning period, there was a slight increase in leukocytes mean of the control group (3940 ± 1549.3) compared to the experimental group (3840 ± 1468.3), whose difference was not significant. After administering the extract of Medicago sativa L to the experimental group, alternating with their respective nutrients, a significant difference was observed between the mean (7800 ± 2885.2) and the control group (3930 ± 1518.1) (p <0.05). Conclusions: Under experimental conditions, the consumption of Medicago sativa extract significantly increased the number of leukocytes. [ABSTRACT FROM AUTHOR]

Introduction: The Gaucher disease is a metabolic disorder due to deficiency or absence of acid β-glucosidase enzyme. The diagnosis is clinically suspected, but requires confirmation by measuring the activity of acid β-glucosidase enzyme in leukocytes (gold standard) or in dried blood on filter paper. Objective: To assess the validity of the measurement of acid β-glucosidase enzyme activity in dried blood on filter paper compared to the standard of gold, for the diagnosis of Gaucher disease in patients with clinical suspicion. Methodology: A systematic review of literature was carried out. A PICO question was constructed and validated. A generic search strategy was used based on the key terms (Gaucher Disease and Dried Blood Spot Analysis). Two independent evaluators reviewed and assessed the quality, and extracted information from the articles. Results: Discarding the duplicates, 47 articles were obtained. Four complete texts were evaluated, and three of them were excluded when applying inclusion and exclusion criteria. The article that was included had an excellent quality and showed that the enzymatic activity of glucocerebrosidase in dried blood had a sensitivity of 82.3%, a specificity of 94.0% with a cut-off of 0.0-2.75, a sensitivity of 88.2% and specificity of 88.5% with a cut-off point of 0.0-4.4. Conclusions: The enzymatic measurement of acid β-glucosidase in dried blood is an excellent test for an initial diagnosis of Gaucher disease; however, it is not a conclusive proof. [ABSTRACT FROM AUTHOR]

The reactive anti-CD45 is useful for the diagnostic and the study of T or B cells immunodeficiency. In this works were assayed three protocols with the purpose to obtain this reagent for the production, each one modifying the relation among the fluorochrome and the antibody (F:P) to conjugate, referred to the proposal of 1 : 2 recommended. The antibody was purified by affinity chromatography with 99,3 % of purity; was evaluated the relation FITC/Protein to 495 nm and 280 nm; and also, was obtained the leukocyte recognition percent through cytometry of flow by three tried protocols which evidence good biological activity (90,7 % - 96,8 %). The best protocol was P-2, with the lowest production cost and highest profits compared with product imported usually used in health system. This study permitted establishes the process to obtain anti-CD45 - FITC conjugated in LABEX laboratories. [ABSTRACT FROM AUTHOR]

Copyright of Agro Productividad is the property of Colegio de Postgraduados and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

There are wild organisms that are highly vulnerable to anthropogenic and natural stressors, these organisms may be useful to assess environmental health through various reliable, simple, rapid, inexpensive indicators and to determine and if possible reduce these negative effects. In this study we propose that the snow goose (Chen caerulescens) to be tested as stress and environmental biomonitor of genotoxicity, by heterophil/lymphocyte (h/l) ratio, micronucleus test (mne), nuclear protrusions in erythrocytes (npe) and polichromatic erythrocytes (pce). During the hunting season (2012-2013) in the wetland of Malaga, Durango, Mexico, 18 organisms were collected. Erythrocytes were the most abundant cells with elliptical morphology and nucleus, size of 12.68 ± 0.89, regular contour and nucleus-cytoplasm ratio 1:2. We observed heterophils (11.07 ± 1.32 μm), eosinophils (9.67 ± 1.26 μm), basophils (5.75 ± 0.78 μm), monocytes (10.49 ± 1.36 μm) and lymphocytes (6.53 ± 1.0 μm). No alteration in the proportions or morphology of leukocytes was identified, however, a greater number of organisms are required to establish normal blood parameters basis for this species. h/l ratio was 0.41±0.11 this value is similar to that reported for this and other bird species classified as healthy. mne basal rate (2.6 ± 1.45), npe (249.2 ± 89.74) and pce (156.5 ± 50) was established. The snow goose is an organism that is emerging as a good candidate for environmental biomonitor due to basal frequency of h/l, mne, npe and pce, but must be tested under standardized conditions and through studies in areas with and without pollution. [ABSTRACT FROM AUTHOR]

Copyright of Revista de Investigaciones Veterinarias del Peru is the property of Universidad Nacional Mayor de San Marcos (UNMSM) and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Ginecología y Obstetricia de México is the property of Federacion Mexicana de Ginecologia y Obstetricia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Revista de Investigaciones Veterinarias del Peru is the property of Universidad Nacional Mayor de San Marcos (UNMSM) and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Revista Cientifica de la Facultade de Veterinaria is the property of Universidad del Zulia, Facultad de Ciencias Veterinarias and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The objective of this work was to study blood parameters in dairy cattle during heat waves. The study was carried out during the 2011-2012 and 2012-2013 summer seasons in the Dairy Module at the College of Agricultural Sciences, located in Zavalla (33°1´S, 60°53´O), in the south of the province of Santa Fe. Levels of creatinine, urea, total protein, serum albumin, as well as haematocrit, leukocyte count, and lymphocyte/neutrophil ratio were determined in blood samples collected from ten lactating second- and third- lactation cows during hot waves. The time of occurrence and length of heat waves were determined using the temperature-humidity index (THI), calculated with weather data from the Zavalla Agro meteorology Station, which belongs to the National Weather Service network and to INTA (National Institute of Agricultural Technology). The minimum and maximum levels of creatinine (0.87 and 1.33 mg/100mL, respectively) remained within normal values compared to standard reference ranges. Slight increases in total protein (6.07 to 9.16 g/100mL) and serum albumin (2.83 to 3.99 g/100mL) occurred when the ITH peaked at 76 during the January heat wave. Urea levels were consistently high in all measurements (17.41-70.95 mg/100mL). Leukocyte levels were always above normal ranges, with a marked decrease in the lymphocyte/neutrophil ratio (57 to 36) during the last heat wave of January. Haematocrit levels presented a decrease (32.09% to 29.92%) characteristic of chronic stress. Our results show that heat waves have a negative effect on the blood parameters of Argentinian Holstein cows in Zavalla. [ABSTRACT FROM AUTHOR]

Copyright of Revista de Investigaciones Veterinarias del Peru is the property of Universidad Nacional Mayor de San Marcos (UNMSM) and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Background: The automated analyzers are mechanism systems that are employed to realize the blood cellular count and leukocyte formula. In these days exist, some analyzers that have an extended differential count, in which includes immature granulocytes, reticulocytes count, and nucleated red blood cells. That's why is important to analyze the precision on these new parameters. Objective: Analyze the performance of Sysmex XT1800i in the immature granulocyte parameter in comparison with the manual count. Material and methods: For an eight week period all the samples analyzed for complete blood count were recollected and only were selected the ones with immature granulocytes alarm. Comparison between automated and manual count was made. Manual count was done according some points of the National Committee for Clinical Laboratory Standards H20-A document. Results: 120 samples were analyzed, in which 20 did not demonstrated the presence of immature granulocytes by the manual count, in this samples Sysmex XT1800i was able to analyze 0.1x10³, which is impossible in a manual differential. The correlation between both methods was r: 0.55. Conclusion: It is necessary the use of the manual count, for the confirmation of these parameter, because the presence of immature granulocytes in the blood film would make us think in an important infectious or inflammatory process. [ABSTRACT FROM AUTHOR]

Copyright of Revista de Investigaciones Veterinarias del Peru is the property of Universidad Nacional Mayor de San Marcos (UNMSM) and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The First published description of a case of leukemia was present by Velpeau in 1827. Eleven years after, Donné described a patient with an excess of white blood cells, and in 1845 a patient with an enlarged spleen in which death took place from the presence of purulent matter in the blood, was reported by J.H Bennett. The term leukemia was coined by Virchow in 1856. Further advances in the understanding of leukemia occurred rapidly thereafter by several scientists like Ehrlich, who developed a technique of staining cells, Neumann, who recognized that the blood cells were made in the bone marrow, an Norwell and Hungerford who described the Philadelphia Chromosome in patients with chronic myelogenous leukemia. In 1947 the pathologist Sidney Farber acquired folic acid antagonists, that were available to him by the biochemist Subbarow, and administered to children with leukemia observing temporary remission. Today, scientific progress like the successful therapeutic application of imatinib in patient with certain forms of leukemia, has provided a proof of target therapy in patients with various types of cancer. [ABSTRACT FROM AUTHOR]

The blood count is one test used for hematology diagnostic assessment of a patient. Hematological values of healthy patients may vary according to individual characteristics and environment of a population. Therefore, the hematology reference intervals vary according to age, sex, height, etc. The aim of this study was to determine reference intervals of blood count in the Mexican population. Results of 654,047 individuals of both genders were analyzed. We used the nonparametric method of T ukey for the detection of extreme values and reference intervals determined by the method nonparametric recommended by the Clinical and Laboratory Standards Institute (CLSI) guide in C28-A3. The results obtained showed the variability of the intervals calculated with respect to the reference intervals suggested by the manufacturer of the following parameters: leukocytes, erythrocytes, hemoglobin, MCV, platelets, neutrophils and lymphocytes, in percentage and absolute values. The displacement and amplitude changes in the estimated range, was between genders and among age groups established, so it is important that each clinical laboratory reference intervals determined in the study population and in their analytical procedures. [ABSTRACT FROM AUTHOR]

Objective. To evaluate the macroscopic agglutination test using Temperature Resistant (TR) antigen as a screening test for the diagnosis of human leptospirosis in different stages of the disease. Materials and methods. The criteria for case definition were based on the results of the microscopic agglutination test (MAT), leukocyte counts and neutrophilia, resulting 218 confirmed cases and 242 non-cases. Each sample was classified according to the days of the disease progression in three stages: first (<10 days), second (10 - 25 days) and third (> 25 days). The design was cross-sectional observational. Results. TR sensitivity was 71,1% on stage 1. 93.4% on stage 2 and 95.6% on stage 3. The specificity at different stages ranged from 79.0 to 69.2%. Intra and inter-operator variability was moderate. Conclusion. TR variability, low sensitivity in the first stage and low specificity found in all stages of the disease, suggest that it is essential to incorporate new diagnostic methods to screen for early detection of cases in our country and in countries that still apply such methods. [ABSTRACT FROM AUTHOR]

The modified techniques for DNA extraction from white blood cells, allow obtaining a good efficiency in the extracted DNA quantity and purity.. Objective: To modify the DNA extraction technique in order to save time and necessary reactives. Materials and Methods: Gustafson's /1987) extraction technique steps were modified. The quantity of DNA produced was calculated using spectrophotometry. The DNA purity was assessed through PCR amplification and confirmed using agarose gels. Results: The changes proposed to Gustafson's DNA extraction method, represent 70% savings in money, and a 6 hours savings in time approximately. Conclusion: The method proposed in the present work is more economic in time and money for white blood cells DNA extraction. Also the efficiency as far as quantity of DNA produced is higher. [ABSTRACT FROM AUTHOR]

Copyright of Iatreia is the property of Universidad de Antioquia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of E-balonmano.com: Journal of Sports Science / Revista de Ciencias del Deporte is the property of Federacion Extremena de Balonmano and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The complete blood count is one of the most commonly ordered blood tests. Also known as full blood exam or hemogram, it is actually a panel of tests that examines different parts of the blood and includes white blood cell count, red blood cell count and platelet count. Many conditions will result in increases or decreases in the cell populations. The following article explains what increases or decreases in each of the components of the white blood cell count may mean [ABSTRACT FROM AUTHOR]

Objective: to determine the differences in sperm morphology between couples who never have been pregnant and those with habitual early pregnancy loss. Methods: forty five infertile couples were divided into two groups as follow: if they have never been pregnant or if they had habitual miscarriage. Women's and men's ages, as well as time of infertility were investigated. A semen analysis was done to all male partners and the following parameters were analyzed: Concentration, agglutination, viscosity, motility, morphology and leukocytes presence. For analysis three cutoff points were used, considering if they had two or more miscarriages, three or more or 4 or more. Data are reported in median and range. Comparison among the groups was made by Mann-Whitney U test and Chi square. Results: when comparing the couples with two or more miscarriages, with those with no pregnancy, no differences were found. When the cutoff point was three or more miscarriages, also no differences were found. When cutoff point was four or more miscarriages, only normal morphology was statistically greater in group with no previous pregnancy than in group with habitual miscarriage 47 (16-93) and 36 (4-40), respectively, p < 0.04). Conclusions: low normal sperm morphology is associated with repeated early pregnancy loss. [ABSTRACT FROM AUTHOR]

Background. Cytopenias secondary to hypersplenism with splenomegaly related to portal hypertension, complicate the clinical course of patients with chronic liver disease and cirrhosis. Partial embolization of the splenic artery, a procedure in development for three decades, is now a therapeutic option that has proven safe, less traumatic and effective for treatment of hypersplenism and splenomegaly consecutive liver cirrhosis with portal hypertension. Objectives. We assessed changes in hematologic cell counts of partial splenic embolization in patients with hypersplenism secondary to chronic liver disease in the Hospital Central Militar. Hypothesis. Partial Splenic Arterial Embolization is a therapeutic procedure that has a positive impact by increasing platelet counts, leukocytes and erythrocytes in patients with chronic liver disease secondary to hypersplenism. Material and methods. To assess the statistical significance in the values obtained from the platelet count, leukocytes and erythrocytes was used nonparametric Kruskal-Wallis test and Mann Whitney. Results. The average values in the platelet count before the procedure was 46.924/mm3, up to seven days after the embolization 93.531/mm3, keeping the average at three months 90.203/mm3 observing a decrease in platelet count at six months with an average of 79.640/mm3, obtained 12 months after the procedure an average of 77.405/mm3 in the number of platelets. An increase in the percentage of platelets to seven days from 94 to 84% three months, six months of 63 and 58% at 12 months after splenic embolization. There was no significant increase in a in the number of leukocytes and erythrocytes. Conclusions. An increase significantly in the number of platelets after partial splenic embolization on day 7, three, six and 12 months. There was no significant increase in leukocyte count and erythrocyte. [ABSTRACT FROM AUTHOR]

Copyright of Revista de Investigaciones Veterinarias del Peru is the property of Universidad Nacional Mayor de San Marcos (UNMSM) and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

White blood cells also known as leukocytes play a significant role in the diagnosis of different diseases. Digital image processing techniques have successfully contributed to analyze the cells, leading to more accurate and reliable systems for disease diagnosis. However, a high variability on cell shape, size, edge and localization complicates the data extraction process. Moreover, the contrast between cell boundaries and the image's background varies according to lighting conditions during the capturing process. On the other hand, Artificial Immune Systems (AIS) have been successfully applied to tackle numerous challenging optimization problems with remarkable performance in comparison to classical techniques. One of the most widely employed AIS approaches is the Clonal selection algorithm (CSA) which allows to proliferate candidate solutions (antibody) that better resolve a de- terminate objective function (antigen). This paper is thus focused on the segmentation, localization and measurement of leukocytes from other different components in blood smear images. The algorithm uses two different CSA systems, one for the segmentation phase and other for the leukocyte detection task. In the approach, the detection problem is considered to be similar to an optimization problem between the leukocyte and its best matched circle shape. On the other hand, Clonal selection algorithm (CSA) is one of the most widely employed AIS approaches. The algorithm uses the encoding of three points as candidate leukocytes over the edge image. An objective function evaluates if such candidate leukocytes are actually present in the edge image following the guidance of the objective function's values. A set of encoded candidate leukocytes are evolved using the CSA so that they can fit into the actual leucocytes shown by the edge map of the image. The obtained results in comparison with the state-of-the-art algorithms validate the efficiency of the proposed technique with regard to accuracy, speed, and robustness. [ABSTRACT FROM AUTHOR]

Copyright of Ginecología y Obstetricia de México is the property of Federacion Mexicana de Ginecologia y Obstetricia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)