1. [In vitro Antiviral Activity of Recombinant Antibodies of IgG and IgA Isotypes to Hemagglutinin of the Influenza A Virus].
- Author
-
Argentova VV, Aliev TK, Zarubaev VV, Klotchenko SA, Shtro AA, Sergeeva MV, Toporova VA, Dolgikh DA, Sveshnikov PG, Vasin VA, and Kirpichnikov MP
- Subjects
- Animals, Antibodies, Neutralizing biosynthesis, Antibodies, Neutralizing genetics, Antibodies, Viral biosynthesis, Antibodies, Viral genetics, Antiviral Agents chemistry, Antiviral Agents metabolism, CHO Cells, Cricetulus, Dogs, Gene Expression, Hemagglutinin Glycoproteins, Influenza Virus genetics, Hemagglutinin Glycoproteins, Influenza Virus immunology, Humans, Immunoglobulin A biosynthesis, Immunoglobulin A genetics, Immunoglobulin G biosynthesis, Immunoglobulin G genetics, Influenza A virus growth & development, Influenza A virus immunology, Madin Darby Canine Kidney Cells, Neutralization Tests, Protein Multimerization, Recombinant Proteins biosynthesis, Recombinant Proteins genetics, Recombinant Proteins pharmacology, Antibodies, Neutralizing pharmacology, Antibodies, Viral pharmacology, Antiviral Agents pharmacology, Immunoglobulin A pharmacology, Immunoglobulin G pharmacology, Influenza A virus drug effects
- Abstract
Seasonal and highly infectious strains of the influenza A and influenza B viruses cause millions of cases of severe complications in elderly people, children, and patients with immune diseases each year. Immunoglobulin A (IgA), which is an active component of humoral immunity, can prevent the spread of the virus in the upper respiratory tract. The preparation and study of the properties of recombinant virus-specific IgA could be an important approach to finding new means of preventing and treating influenza. Based on CHO DG44 cells, we developed stable monoclonal cell lines that produce monomeric and dimeric antibodies FI6-IgA1 and FI6-IgA2m1 to hemagglutinin (HA) of the influenza A virus. When studying the productivity, growth, and stability of the obtained clones, we found that the dimeric form of antibodies of IgA1 isotype is superior to other forms. The dimeric form of IgA antibodies plays a key role in mucosal immunity. Recognizing the prospects of using dimeric IgA as prophylactic and therapeutic mucosal drugs for viral infections, we studied their virus-neutralizing and antiviral activities on MDCK cell culture and compared them with the antibodies of the IgG1 isotype. This study presents the data on antiviral and virus-neutralizing activities of the FI6-IgA1 dimers to seasonal and highly infectious strains of influenza A virus.
- Published
- 2017
- Full Text
- View/download PDF