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[Mitochondrial ceruloplasmin of mammals].
- Source :
-
Molekuliarnaia biologiia [Mol Biol (Mosk)] 2005 Jan-Feb; Vol. 39 (1), pp. 48-60. - Publication Year :
- 2005
-
Abstract
- Using immunoblotting method it was found out that ceruloplasmin (Cp) polypeptides are revealed in mitochondria of rats, isolated from brain, liver, testicles and mammary gland. Cp is localized in matrix and inner membranes of mitochondria. Its molecular weight corresponds to the non-glycosilated form of the protein. Computer analysis did not reveal any sequences homologous to the signal peptide for mitochondria protein import (SPMPI) in the rat chromosomal Cp gene. However the analysis of homologous to Cp sequences, presented in databases, detected SPMPI in the human processed Cp pseudogene. Cp pseudogene region of 984 nucleotides long is translated in the only reading frame to the polypeptide of 328 a.a. long including 66 a.a of SPMPI at N-terminus. The predicted protein with the exception of SPMPI is identical to the corresponding Cp fragment at 92%, it has 20 amino acid substitutions, 8 of which are significant. His-X-His motif, typical for copper containing ferroxidases, is located in the centre of a molecule. Potential copper-binding site appears as a result of proline to histidine substitution at 923 position along Cp sequence. The product of Cp pseudogene transcription was detected in the human cultured cells of HepG2 and HuTu 80 cell lines using RT-PCR strategy. 30 kDa polypeptide that interacts with human Cp antibodies was found in mitochondria of HuTu 80 cells. The possible biological role of mitochondrial Cp is under discussion.
- Subjects :
- Amino Acid Sequence
Animals
Cell Fractionation
Cell Line, Tumor
Ceruloplasmin genetics
Copper metabolism
Humans
Immunoblotting
In Vitro Techniques
Intracellular Membranes metabolism
Models, Molecular
Molecular Sequence Data
Organ Specificity
Protein Sorting Signals physiology
Pseudogenes
RNA, Messenger metabolism
Rats
Ceruloplasmin metabolism
Mitochondria metabolism
Protein Sorting Signals genetics
Subjects
Details
- Language :
- Russian
- ISSN :
- 0026-8984
- Volume :
- 39
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Molekuliarnaia biologiia
- Publication Type :
- Academic Journal
- Accession number :
- 15773547