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O objetivo deste trabalho foi comparar dois protocolos de hipofisação, na indução à desova de curimatã-pacu.Testou-se a aplicação de dose única ou duas doses de extrato bruto de hipófise de carpa-comum. Não houve diferença significativa entre os protocolos quanto às taxas de fêmeas que desovaram, fertilização e larvas deformadas. A aplicação de dose única resultou em valores de horas-graus até a extrusão dos ovócitos maiores do que em duas doses. A aplicação de uma única dose representou menor consumode água, energia e tempo. O protocolo da dose única pode ser utilizado como alternativa para a indução à desova de curimatã-pacu. The objective of this work was to compare two hypophysation protocols in the spawning induction of curimatã-pacu. The application of a single dose or two doses of crude carp pituitary extract was tested. That there was no significant difference among the protocols for the rate of females that have spawned, fertilization and deformed larvae. A single dose application resulted in higher degree-hour values until the oocyte extrusion than two doses. The application of a single dose represented a lower consumption of water, energy, and handling time. A single dose protocol can be used as an alternative for inducing the curimatã-pacu spawning.

The marine shrimp Litopenaeus vannamei may be reared in a wide range of salinity (0.5-60.0 g L-1). In this study, the production data of shrimp reared either in oligohaline (0.5-5.0 g L-1) or salty waters (25.0-40.0 g L-1) were compared using mathematical models. The models considered variables related to management and production, and were formulated using a database with 278 culture cycles from different shrimp farms in Northeastern Brazil. The least-squares technique was applied to estimate the model’s parameters. The selection of variables used the Stepwise process associated to Box and Cox’s transformation. The adequacy of the equations and the normality estimated for the errors were analyzed on the basis of the analysis of variance in the Durbin-Watson statistics, on the residue analysis and the normality test. For all the formulated equations, the variable ‘culture in salty water’ was selected, evidencing significant differences between these culture systems. O camarão marinho Litopenaeus vannamei pode ser cultivado em águas com ampla faixa de salinidade (0,5-60 g L-1). Desta forma, objetivou-se comparar dados de produção desse crustáceo quando cultivado em águas oligohalina (0,5-5,0 g L-1) ou salgada (25,0-40,0 g L-1), utilizando-se modelos matemáticos. Para relacionar as variáveis de manejo com as de produção, os modelos foram formulados com base em um banco de dados com 278 cultivos comerciais da região Nordeste do Brasil. Para estimar os parâmetros dos modelos, utilizou-se a técnica dos mínimos quadrados. A seleção das variáveis foi realizada com o processo de Stepwise associado à transformação de Box e Cox. A adequação das equações e os pressupostos de normalidade, para os erros, foram analisados com base na análise de variância, na estatística de Durbin-Watson, na análise de resíduo e no teste de normalidade. Em todas as equações formuladas, a variável cultivo em água salgada (CAS) foi selecionada, evidenciando diferença significativa (p < 0,05) entre esses sistemas de cultivo.

Sodium metabisulfite is the most applied preservative in shrimp culture, with its use supported by current legislation; however, its residue, sulfur dioxide (SO2), in high concentrations can provoke adverse health reactions. This study evaluated the influence of heating under different methods of residual SO2) titration in em>Litopenaeus vannamei. Shrimps were treated with nine concentrations of sodium metabisulfite and evaluated by different methods of SO2) titration. The adapted Adolfo Lutz titration method presented the highest sensitivity for the detection of SO2). The method of iodometric titration with heating made possible the detention of higher levels of SO2), when compared to the same method without heating. The heating influenced negatively on the reactive ribbon method. It is concluded that the heating influenced positively on the iodometric titration, as opposed to the reactive ribbon. O metabissulfito de sódio é o conservante mais aplicado na carcinicultura, com uso amparado em legislações vigentes no país, todavia, seu resíduo, dióxido de enxofre (SO2), em concentrações altas, pode provocar reações adversas à saúde. Este estudo teve como finalidade observar a influência do aquecimento sob diferentes métodos de titulação de SO2) residual em camarões Litopenaeus vannamei. Os camarões foram submetidos a nove concentrações de metabissulfito de sódio e avaliados diferentes métodos de detecção de SO2). O método da titulação Adolfo Lutz adaptado apresentou grande sensibilidade para detecção de SO2) residual. O método da titulação iodométrica com aquecimento possibilitou a detecção de maiores níveis de SO2), quando comparado ao método sem o aquecimento. O aquecimento interferiu negativamente no método da fita reativa. Conclui-se que o aquecimento influenciou positivamente a titulação iodométrica, não ocorrendo o mesmo com a fita reativa.

The cultivated shrimp comes if highlighting as one of the main responsible items for the superavit of the Brazilian’s fish trade balance. Its drainage has been accomplished for North America and Europe, where the quality demands is extremely high, especially for microbial load existent at shrimp. Given the demands of importers, the aim was to evaluate the effectiveness of conservator sodium metabisulfite as an antimicrobial agent in the shrimp culture. Shrimps Litopenaeus vannamei classified as 81/100 (10 to 12 g) were submitted to 10 concentrations of sodium metabisulfite (from 1 to 10%), during 10 and 20 minutes. The aerobic mesophilic bacteria were counted and the isolated colonies were submitted to a bacterioscopic test using the Gram method and biochemical tests. It was observed that the number of colony-former units decreased due to metabisulfite use, thus demonstrating its inhibitory effect on the aerobic mesophilas bacteria at the concentrations used. O camarão cultivado destaca-se como um dos principais itens responsáveis pelo superávit da balança comercial do pescado brasileiro. Seu escoamento tem sido realizado para a América do Norte e Europa, onde as exigências de qualidade, especialmente quanto à carga microbiana no camarão, são extremamente altas. Dadas as exigências dos importadores, objetivou-se avaliar a eficácia do conservante metabissulfito de sódio como agente antimicrobiano na carcinicultura. Camarões Litopenaeus vannamei de classificação 81/100 foram submetidos a 10 concentrações do metabissulfito de sódio (de 1% a 10%), durante 10 e 20 minutos. Foram realizadas contagens de bactérias aeróbias mesófilas, sendo as colônias isoladas submetidas ao teste bacterioscópico pelo método de Gram e a provas bioquímicas. Foram isoladas sete espécies de víbrio e observou-se que o número de unidades formadoras de colônias decresceu em função do uso de metabissulfito, ficando demonstrada a sua ação inibitória sobre as bactérias mesófilas aeróbias em todas as concentrações utilizadas.

Litopenaeus vannamei postlarvae with 9-10 (PL9-10), 25-26 days old (PL25-26) were acclimated to freshwater (0 ppt) for the purpose of attending the inland cultures. Two experiments were conducted in 44 liter aquariums with stocking density of 11.36 postlarvae/L. In experiment 01, different concentrations of hydrated lime (0.018 g a 0.031 g) and two diets, formulated with commercial shrimp ration and artemia biomass, were tested. In experiment 02, five diets, with different concentrations of artemia biomass (0 to 100%) inserted in fish and shrimp rations, were tested. At the end of the acclimatization process, the postlarvae survival varied from 40.44 to 98.04%. Results showed that Litopenaeus vannamei postlarvae can be acclimated to freshwater. Also, the increase of lime concentration and the artemia biomass in the ration improved (p < 0.05) the zootechnic parameters of the species in a significant way. Pós-Larvas do camarão Litopenaeus vannamei, na fase de PL9-10 e PL25-26 foram aclimatadas à água doce (0,0 ‰), objetivando promover cultivos em regiões interioranas. Para isso, foram realizados dois experimentos conduzidos em aquários com capacidade útil de 44 litros e densidade de estocagem de 11,36 pós-larvas/L. No experimento 1, foram testadas diferentes concentrações de cal hidratada (0,018 g a 0,031 g) e 2 dietas, formuladas à base de ração comercial para camarão e biomassa de artêmia. No experimento 2, foram testadas 5 dietas com diferentes concentrações de biomassa de artêmia (0% a 100%), inseridas em rações de peixes e camarões. No final do processo de aclimatação, a sobrevivência das póslarvas variou de 40,44% a 98,04%. Concluiu-se que pós-larvas do Litopenaeus vannamei podem ser aclimatadas à água doce e que o aumento da concentração de cal e de artêmia na ração melhoraram significativamente (p < 0,05) os parâmetros zootécnicos da espécie.

Experiments were carried out in a commercial marine shrimp farm in order to evaluate the use of two diets in Litopenaeus vannamei post-larvae, during nursery phase, on physiochemical wastewater quality. Two feeding methods were adopted: Commercial Feeding Method (MAC) and Artemia Feeding Method (MAA).0 The nursery tanks (60 m3) were provided with 19 days post-larvae (PL19) with a stocking density of 16PL/liter. After 10 days of culture, the concentrations of nitrogen (ammonia, nitrite and nitrate) and phosphorus compounds (total phosphorus and orthophosphate) in the nursery tank were smaller for MAC, rather than MAA treatment (p < 0.05). Consequently, MAA treatment induced low levels of daily compounds increment, thus contributing to a significant reduction of the nutrients that caused water eutrophication. Cultivos experimentais com pós-larvas do Litopenaeus vannamei, na fase de berçário, foram realizados em uma fazenda de camarão, objetivando avaliar a influência da utilização de duas dietas na qualidade físico-química das águas residuais. As dietas foram formuladas à base de ração comercial e náuplios de artêmia, denominadas MAC e MAA, respectivamente. Foram utilizadas pós-larvas no estágio (PL19), estocadas a 16PL/L em dois tanques-berçário de 60 m3. Ao final de 10 dias de cultivo, as concentrações dos compostos nitrogenados (amônia, nitrito e nitrato) e fosfatados (fósforo total e ortofosfatos) dos tanquesberçário foram menores na dieta MAA do que na MAC (p < 0,05). Conseqüentemente, a dieta MAA induziu menores taxas de incremento diário desses compostos, contribuindo com uma redução significativa dos nutrientes responsáveis pela eutrofização da água.

The fish consumption has grown, either for a higher sense of the consumers or for governments marketing strategies. In this context, the Japanese culinary is distinguished, whose main basis is fish. This study aims to evaluate the sashimi and vegetables commercialized in restaurants of Recife through Most likely Number (NMP) of Vibrio parahaemolyticus, plus total and termotolerantes coliforms, research of Vibrio spp. and Escherichia coli. 30 samples collected in five establishments were analyzed. Significant difference in the indexes of contamination of the searched restaurants was not observed. Presence of Escherichia coli was verified in two samples of sashimi and eight vegetable samples. damsela, V. fluvialis, V. furnissii, V. hollisae and V. harveyi were identified, not being isolated V. parahaemolyticus. One conclusion was that some restaurants need to implement Good Fabrication Practices (BPF), so that the commercialized sashimi and vegetables do not propagate microorganisms, minimizing the incidence of the illnesses transmitted by food. O consumo de pescado tem crescido, seja pela maior consciência dos consumidores, seja por estratégias de marketing dos governos. Nesse contexto, destaca-se a culinária japonesa, cuja base principal é o pescado. Desta forma, objetivou-se avaliar os sashimis e vegetais Comercializados em restaurantes do Recife através do Número Mais Provável (NMP) de Vibrio parahaemolyticus e coliformes totais e termotolerantes, pesquisa de Vibrio spp. e Escherichia coli. Analisaram-se 30 amostras coletadas em cinco estabelecimentos. Não se observou diferença significativa nos índices de contaminação dos restaurantes pesquisados. Verificou-se presença de Escherichia coli em duas amostras de sashimi e em oito amostras de vegetais. Identificaram-se Vibrio damsela, V. fluvialis, V. furnissii, V. hollisae e V. harveyi, não sendo isolado V. parahaemolyticus. Conclui-se que alguns restaurantes necessitam implantar as Boas Práticas de Fabricação (BPF) para que os sashimis e vegetais comercializados não veiculem microrganismos, diminuindo a incidência das doenças transmitidas por alimentos.

Experiments were carried out in a commercial marine shrimp farm to evaluate growth and survival rate of the Litopenaeus vannamei post-larvae during nursery phase using different diets. The nursery tanks (60 m3) will be provided with post-larvae of 19 days (PL19) with a density of 16 PL/liter. The post-larvae were submitted to a Commercial Feeding Method (MAC) and an Artemia Feeding Method (MAA). Considering the correlations between weight and length, and between weight and culture duration, a better efficiency (p Experimentos foram realizados em uma fazenda comercial de criação de camarões marinhos, objetivando avaliar a taxa de crescimento e sobrevivência das pós-larvas do Litopenaeus vannamei, quando cultivadas em tanques-berçário e submetidas a diferentes dietas. As pós-larvas utilizadas encontravam-se com 19 dias (PL19) e foram estocadas a 16 PL/L, em tanques-berçário de 60 m3. As pós-larvas foram submetidas ao Método de Alimentação Convencional (MAC) e o Método de Alimentação com Artêmia (MAA). Verificou-se que ao se correlacionar o peso em função do comprimento e do tempo de cultivo, o MAA apresentou uma relação estatisticamente superior (p

Submitted by Mario BC (mario@bc.ufrpe.br) on 2017-11-06T13:13:02Z No. of bitstreams: 1 Fabiana Penalva de Melo.pdf: 1100633 bytes, checksum: dc4f05055f2d72945a79e10b88bdcbb4 (MD5) Made available in DSpace on 2017-11-06T13:13:02Z (GMT). No. of bitstreams: 1 Fabiana Penalva de Melo.pdf: 1100633 bytes, checksum: dc4f05055f2d72945a79e10b88bdcbb4 (MD5) Previous issue date: 2016-02-25 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES The accumulation of inorganic toxic substances such as ammonia and nitrite, is a major water quality problem in intensive aquaculture systems. Often the levels of nitrogen compounds in aquaculture systems or in the nature, exceed the values of LC50, although, experiments on exposure subchronic/chronic nitrogenous compounds on the growth and/or feeding rates are relatively limited. Thus, the aim of this study was to evaluate the toxicity of nitrite on survival, food consumption and immune response of Litopenaeus vannamei shrimp grown in the biofloc technology and clear water. To evaluate the toxicity of the nitrite on growth, survival and total amount of hemocytes in the hemolymph of L. vannamei juveniles. Four concentrations of nitrite (0, 10, 20 e 40 mg N-NO2 L-1) were tested in two culture systems - clear water and biofloc for 30 days. Survival of shrimp was negatively influenced (P

Submitted by (edna.saturno@ufrpe.br) on 2017-02-23T14:03:29Z No. of bitstreams: 1 Juliana Maria Aderaldo Vidal.pdf: 784041 bytes, checksum: f927beb63f5c3c304d4098214b0dd629 (MD5) Made available in DSpace on 2017-02-23T14:03:29Z (GMT). No. of bitstreams: 1 Juliana Maria Aderaldo Vidal.pdf: 784041 bytes, checksum: f927beb63f5c3c304d4098214b0dd629 (MD5) Previous issue date: 2015-07-27 Bacteria with probiotic effect have been used in marine shrimp production like antibiotics substitution, contributing to the health of the host, by antagonistic action to pathogenic micro-organisms or by competition for space and nutrients, and improving appetite and lead the further growth of the animals. When isolated from the host itself is safe to use, being able to adhere and colonize the intestine. The objective was to isolate probiotic bacteria from intestine of juvenile Litopenaeus vannamei and evaluate the effects of probiotic administration in the performance of cultivated animals experimental challenged infection. Intestinal bacteria were from cultivated shrimp in oligohaline water and saltwalter in dry and rainy seasons, and confronted with Vibrio parahaemolyticus, Vibrio vulnificus, Vibrio alginolyticus and Aeromonas hydrophila. Bacteria showed antimicrobial effect have been identified by molecular biology. Twenty-nine isolates showed antagonistic effect at least one of the tested pathogens. The species that most occurred was Bacillus cereus, the producing of the largest zones of inhibition to the V. alginolyticus and V. vulnificus. The bacterium Citrobacter freundii, from oligohaline water showed the best antimicrobial effect before the four pathogens. The probiotic Bacillus cereus was tested in diet for marine shrimp post-larvae against challenge with V. parahaemolyticus, and V. alginolyticus which evaluated the growth performance of animals, colonization capacity of the probiotic bacteria, pathogens count and histopathological lesions. The use of probiotic had no effect on animals survival, but not for treatments which was used probiotic had a lower weight gain. Animals fed dietary supplementation of probiotic had lower pathogens count those fed without the use. Histopathological lesions were observed in organs and tissues of animals. It can be concluded that it was possible to isolate bacteria having probiotic effect of marine shrimp intestine, wherein the strain Bacillus cereus demonstrated high capacity to colonize the host itself, causing a significant reduction of pathogens. Bactérias com efeito probiótico contribuem para saúde de camarões marinhos em cultivo, em substituição aos antibióticos, exercendo ação antagonista à micro-organismos patogênicos ou por competição por espaço e nutrientes, além de melhorar o apetite levando a um maior crescimento dos animais. Quando isolados do próprio camarão seu uso é seguro, por serem capazes de aderir ou colonizar o intestino destes. Neste sentido, objetivou-se isolar bactérias probióticas do intestino de juvenis de Litopenaeus vannamei e avaliar os efeitos da sua administração sobre o desempenho dos animais cultivados frente infecção experimental. Foram isoladas bactérias do intestino de camarão cultivado em águas oligohalina e salgada, em períodos seco e chuvoso, e confrontadas com Vibrio parahaemolyticus, Vibrio vulnificus, Vibrio alginolyticus e Aeromonas hydrophila, em testes in vitro. As bactérias que produziram efeito antimicrobiano foram identificadas através de biologia molecular.Vinte e nove isolados apresentaram efeito antagônico a pelo menos um dos patógenos testados. A espécie que mais ocorreu foi Bacillus cereus, produzindo os maiores halos de inibição frente ao V. alginolyticus e V. vulnificus. A bactéria Citrobacter freundii, proveniente de água oligohalina apresentou melhor efeito antimicrobiano, perante os quatro patógenos. O probiótico Bacillus cereus foi testado em ração para pós-larvas de L. vannamei, frente a desafio com V. parahaemolyticus, e V. alginolyticus quando avaliou-se o desempenho zootécnico dos animais, a capacidade de colonização das bactérias probióticas, contagem de patógenos e lesões histopatológicas. O uso do probiótico não influenciou nas taxas de sobrevivência dos animais, porém nos tratamentos em que não se utilizou probiótico houve menor ganho de peso. Os animais que receberam ração suplementada de probiótico, tiveram contagem de patógenos inferior aqueles alimentados sem o uso. Não foram observadas lesões histopatológicas nos órgãos e tecidos dos animais. Concluiu-se que é possível isolar bactérias com efeito probiótico do intestino do camarão marinho, sendo que a cepa de Bacillus cereus demonstrou maior capacidade de colonizar o próprio hospedeiro, diminuindo os patógenos.

Submitted by (edna.saturno@ufrpe.br) on 2016-10-20T14:50:29Z No. of bitstreams: 1 Monique Monteiro Pinto.pdf: 848527 bytes, checksum: 1b2bc1d9050d7a02d03c3c3faf35a485 (MD5) Made available in DSpace on 2016-10-20T14:50:29Z (GMT). No. of bitstreams: 1 Monique Monteiro Pinto.pdf: 848527 bytes, checksum: 1b2bc1d9050d7a02d03c3c3faf35a485 (MD5) Previous issue date: 2013-02-27 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES Probiotics are responsible for a new idea of using preventive in aquaculture, aiming at sustainable farming, environmental balance and of its products generated. However, there are few knowledge on the subject, despite its widespread use in shrimp farming, randomly in the most of times. Because of large demand from the sector for new alternatives, to maintain animal health, reduce the chance of having disease problems that the study aimed to identify and test bacteria with potential probiotic isolated from the intestinal tract of cultured shrimp (Litopenaeus vannamei) in salt water coming from commercial farm in the state of Pernambuco. Has been collected 90 animals, 50 in rainy season and 40 in dry season. The shrimps collected during the rainy season had weight of 9.50 ± 0.52 g and length of 6.72 ± 0.84 cm, while the animals from the dry period had weight of 9.76 ± 0.24 g and a length of 6, 54 ± 0.42 cm. Were obtained 72 bacterial isolates then performed the Gram 31 were tested in vitro against dual-known pathogenic species, Vibrio parahaemolyticus (ATCC 17802) and Vibrio vulnificus (ATCC 27562). Eight isolates (28.81%) showed antibacterial activity to at least one pathogen. There was no significant difference (P

Submitted by (edna.saturno@ufrpe.br) on 2017-02-08T15:13:02Z No. of bitstreams: 1 Bruna Larissa Ferreira de Carvalho.pdf: 1085105 bytes, checksum: 508758721a8072d306835fa150dcd0e1 (MD5) Made available in DSpace on 2017-02-08T15:13:02Z (GMT). No. of bitstreams: 1 Bruna Larissa Ferreira de Carvalho.pdf: 1085105 bytes, checksum: 508758721a8072d306835fa150dcd0e1 (MD5) Previous issue date: 2013-02-25 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES The accumulation of nitrite is one of the major constraint on water quality in system biofloc. This nitrogen compound is very toxic to aquatic organisms and systems without water exchange, concentrations can reach lethal levels. This study was divided into two experiments. The first objective was to evaluate the contribution of prior addition of sodium nitrite (NaNO2) in the formation of nitrogenous compounds in system biofloc with Litopenaeus vannamei. It was used a completely randomized design with two treatments and eight replications: 1) with the addition of sodium nitrite to 10 days before storage (NaNO2) and 2) without the addition of sodium nitrite (CTL). The post-larvae (0.143 ± 0.001 g) were stocked in 50 L tanks at a density of 1 PL L-1 for 28 days. Daily we measured physical and chemical parameters of the water weekly and ammonia, nitrite, nitrate, alkalinity and volume of flocs. The second experiment aimed to determine the acute toxicity of nitrite in post-larvae (0.012 ± 0.03 g) and juveniles (2.49 ± 0.20 g) for L. vannamei in salinity 30. Experimental units were used with a volume of 10 L and 1.6 L for post-larvae and juveniles respectively. The experiment was conducted in the following concentrations: 0, 20, 40, 80, 160, 240, 320, 400 and 480 mg NO2-N L-1. We determined the median lethal concentration (LC50) for post-larvae and juveniles in 24, 48, 72 and 96 h. In the first experiment, the addition of NaNO2 may have accelerated the formation of nitrate, however the other variables did not influence water quality. There was no difference between treatments for the results of zootechnical performance of shrimp. The survival data for both treatments were above 95%. In the second experiment, the values of lethal concentration (LC50) of nitrite in post-larvae and juveniles for 24, 48, 72 and 96 h were respectively 224.6, 109.43, 41.16, 25.52 mg NO2-N L-1, and 273.51, 147.97, 113.97, 83.78 mg NO2-N L-1. According to the results of LC50 was possible to estimate the levels of security for post-larval and juvenile L. vannamei at 2.55 and 8.38 mg NO2-N L-1. O acúmulo de nitrito é um dos principais entraves na qualidade da água em sistema de biofloco. Este composto nitrogenado é bastante tóxico para os organismos aquáticos e em sistemas sem troca de água, as concentrações podem chegar a níveis letais. O presente estudo foi dividido em dois experimentos. No primeiro, objetivou-se avaliar a contribuição da adição prévia de nitrito de sódio (NaNO2) na formação dos compostos nitrogenados em sistema de biofloco com Litopenaeus vannamei. Foi adotado um delineamento inteiramente casualizado com dois tratamentos e oito repetições: 1) com adição de nitrito de sódio 10 dias antes da estocagem (NaNO2) e 2) sem adição de nitrito de sódio (CTL). As pós-larvas (0,143 ± 0,001 g) foram estocados em tanques de 50 L na densidade de 1 PL L-1, durante 28 dias. Diariamente foram mensurados os parâmetros físico-químicos da água e semanalmente amônia, nitrito, nitrato, alcalinidade e o volume dos flocos. O segundo experimento teve como objetivo verificar a toxicidade aguda do nitrito em pós-larva (0,012 ± 0,03 g) e juvenis (2,49 ± 0,20 g) para o L. vannamei em salinidade 30. Foram utilizadas unidades experimentais com volume útil de 1,6 e 10 L, para pós-larvas e juvenis, respectivamente. O experimento foi realizado nas seguintes concentrações 0, 20, 40, 80, 160, 240, 320, 400 e 480 mg NO2-N L-1. Foram determinadas as concentrações letais medianas (LC50) para pós-larva e juvenis em 24, 48, 72 e 96 h. No primeiro experimento, a adição de NaNO2 pode ter acelerado a formação de nitrato, entretanto não influenciou as demais variáveis de qualidade de água. Não houve diferença entre os tratamentos para os resultados de desempenho zootécnicos dos camarões. Os dados de sobrevivência para ambos os tratamentos estiveram acima de 95%. No segundo experimento, os valores de concentração letal (LC50) de nitrito em pós-larvas e juvenis para 24, 48, 72 e 96 h foram respectivamente 224,6, 109,43, 41,16, 25,52 mg NO2-N L-1; e 273,51, 147,97, 113,97, 83,78 mg NO2-N L-1. De acordo com os resultados de LC50 foi possível estimar os níveis de segurança para pós-larva e juvenil de L. vannamei em 2,55 e 8,38 mg NO2-N L-1.

Submitted by (edna.saturno@ufrpe.br) on 2017-02-09T12:13:39Z No. of bitstreams: 1 Carolina Notaro de Barros.pdf: 471792 bytes, checksum: 565309c707bb10b4d250687b7f4c6182 (MD5) Made available in DSpace on 2017-02-09T12:13:39Z (GMT). No. of bitstreams: 1 Carolina Notaro de Barros.pdf: 471792 bytes, checksum: 565309c707bb10b4d250687b7f4c6182 (MD5) Previous issue date: 2012-02-08 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES The indiscriminate use of antimicrobial drugs in aquaculture may cause development of drug-resistant bacteria, which become more difficult to be controlled and eliminated. Probiotics may represent an alternative prophylactic disease control, replacing the use of antibiotics. In this respect, this study isolated, tested and identified potential probiotic bacteria from the gut of cobia, Rachycentron canadum, a potential candidate for marine aquaculture. 40 animals were captured, 10 from a private hatchery and 30 from an offshore culture system (PE, Brazil) between November 2010 and July 2011. Fishes from the hatchery had weight of 139.30 ± 31.52 g and length of 27.13 ± 1.46 cm, while those from offshore culture system weighed 456.77 ± 264.46 g and had length of 37.29 ± 6.05 cm. 45 bacterial were isolated and tested in vitro against five known pathogenic species, Aeromonas hydrophila (IOC/FDA 110-36), Pseudomonas aeruginosa (ATCC 15442), Streptococcus agalactiae (ATCC 13813), Vibrio parahaemolyticus (ATCC 17802) and Vibrio vulnificus (ATCC 27562). Fifteen strains (33.33%) had antibacterial activity to at least one pathogen, while eight (17.77%) were inhibited all pathogens tested. There was significant difference (P

Submitted by (edna.saturno@ufrpe.br) on 2017-02-13T11:45:58Z No. of bitstreams: 1 Fabiana Penalva de Melo.pdf: 587502 bytes, checksum: 899b9b2cc09b866a034d428f2e22e935 (MD5) Made available in DSpace on 2017-02-13T11:45:58Z (GMT). No. of bitstreams: 1 Fabiana Penalva de Melo.pdf: 587502 bytes, checksum: 899b9b2cc09b866a034d428f2e22e935 (MD5) Previous issue date: 2012-02-03 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES This study aimed to evaluate the culture of Litopenaeus vannamei marine shrimp fed with different protein levels diets in heterotrophic systems with and without probiotic addition. It was adopted a completely randomized design with 4 x 2 factorial arrangement, using four dietary protein levels (200, 250, 300 e 350 g Kg-1 CP), as the first factor (P20, P25, P30 and P35), and probiotic addition in the water, as the second factor (P20Pro, P25Pro P30Pro e P35Pro). For this were used 24 fiberglass tanks (800 L working volume), filled with a mixture of 300 liters of seawater (30 g L-1), chlorinated and dechlorinated, and 500 liters of seawater from the before culture. Each tank was stocked with 240 juveniles (300 shrimp m-3) averaging 1.55±0.1 g. Water temperature, dissolved oxygen, pH and salinity were measured daily, while NH3-N, NO2-N, NO3-N, PO4-P and alkalinity were analyzed weekly. There were no significant differences in daily parameters of water quality (temperature, dissolved oxygen, pH and salinity; P>0.05), while the weekly parameters only nitrite was influenced by the protein levels (P

Submitted by (edna.saturno@ufrpe.br) on 2017-02-14T16:23:03Z No. of bitstreams: 1 Jones Santander Neto.pdf: 1353954 bytes, checksum: 3ab25dad19a919fb57b060eb783f2097 (MD5) Made available in DSpace on 2017-02-14T16:23:03Z (GMT). No. of bitstreams: 1 Jones Santander Neto.pdf: 1353954 bytes, checksum: 3ab25dad19a919fb57b060eb783f2097 (MD5) Previous issue date: 2011-07-08 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES From October 2007 to March 2011, 105 silky sharks, Carcharhinus falciformis (Muller e Henle, 1839), (68–274.2 cm in total length, TL) were collected from industrial fleet (longline) carried out on Southwestern Tropical Atlantic (010º W/ 035º W, 05º N/ 30º S). Vertebral sections from 34 males (105–268.2 cm TL), 35 females (85–274.2 cm TL), and 36 individuals of unknown sex (68.8-256.1 cm TL) were analyzed. One 68.8 cm TL individual did not have birth ring. The index of average percentage of error (IAPE) estimated from two readings ranged from 0 to 7.69 (mean=3.88%) in the vertebrae of specimens with 1 and 14 band pairs, respectively. There were no differences (p 0.05) in the monthly marginal increment (MIR) analysis between months then we considered an annual ring deposition as other species of the Carcharhinidae family, including Carcharhinus falciformis. Ages range from 0 to 20 years. There were no differences (p 0.05) in growth between sexes. Models derived from von Bertalanffy, particularly the VBGM two phase, best represented growth for the species, which was evidenced by the lowest Akaike information criterion value. Growth parameters estimated for both sexes were L = 303 cm, k = 0.086, t0= -4.71, h=0.17, and th=11.62 with the VBGM two phase model, and L = 292 cm, k = 0.089, and t0= -4.87 with the VBGM. The VBGM was used to other analysis. Males and females mature at 10.8 and 12.5 years of age, repectively, and the age that attain 95% of the L is 30.3 years. Tubarões lombo preto, Carcharhinus falciformis (Muller e Henle, 1839) foram capturados entre outubro de 2007 a março de 2011 pela frota industrial atuando com espinhel pelágico no Atlântico sudoeste Tropical (010º W/ 035º W, 05º N/ 30º S). Foram coletadas vértebras de 105 exemplares, sendo 34 machos (105–268,2 cm CT), 35 fêmeas (85–274,2 cm CT) e 36 indivíduos de sexo não identificado (68,8-256,1 cm CT). Um indivíduo em vida livre medindo 68,8 cm CT não apresentou marca de nascimento. O índice de porcentagem de erro médio (IAPE) estimado entre dois leitores variou de 0% a 7,69% (média= 3,88%) nas vértebras de espécimens com 1 e 14 pares de bandas, respectivamente. Ao analisar o incremento marginal relativo (IMR) não se verificou diferenças (p 0,05) entre os meses, logo assumiu-se a deposição de um anel anual como utilizado para as demais espécies da família Carcharhinidae, inclusive C. falciformis. Idades variaram de 0 a 20 anos. Não houve diferenças (p 0,05) no crescimento entre os sexos. Modelos derivados de von Bertalanffy, particularmente VBGM duas fases, melhor representou o crescimento para a espécie, que foi evidenciado pelo menor valor do critério de informação de Akaike. Parâmetros de crescimento estimado foram L = 303 cm, k = 0,086, t0= 4,71, h=0,17 e th=11,62 com o VBGM duas fases e L = 292 cm, k = 0,089, and t0= -4,87 com o VBGM. O VBGM foi utilizado para as demais análises. Machos e fêmeas maturam com 10,8 e 12,5 anos, respectivamente e a idade com que atingem 95% do L é 30,3 anos.

Submitted by (edna.saturno@ufrpe.br) on 2016-11-08T16:25:01Z No. of bitstreams: 1 Veronica Arns Silva.pdf: 798716 bytes, checksum: 9cb78ed7b4e51b894bc4cd0afcfd8bce (MD5) Made available in DSpace on 2016-11-08T16:25:01Z (GMT). No. of bitstreams: 1 Veronica Arns Silva.pdf: 798716 bytes, checksum: 9cb78ed7b4e51b894bc4cd0afcfd8bce (MD5) Previous issue date: 2011-02-28 Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPq Shrimp farm can be affected both by non-infectious diseases caused by abiotic agents, as for infectious diseases, and among these viral diseases were responsible for the largest losses in shrimp farming. Small and medium shrimp were collected from four commercial farms in the coastal state of Pernambuco, in the periods of summer and rainy, with the aim to investigate the frequency of diseases in these animals, with the aim to investigate the frequency of diseases in these animals. The investigation of viruses Infectious myonecrosis (IMNV), and Infectious Hipodermal and Hematopoietic Necrosis (IHHNV) and White Spot Syndrome (WSSV) was performed by macroscopic analysis, using wet mount, microscopically for histopathology, and analysis molecular (Polymerase Chain Reaction - PCR and nested PCR), identifying the presence or absence of etiological agents. Viruses causing IHHNV IMNV and were detected as lesions suggestive of these infections were more frequent during the dry season. WSS virus was not detected by nested PCR in the specimens, as well as injuries that just allowed the suspected virus circulation during the study period. Lesions suggestive of bacterial diseases, enteritis hemocitical, as well as the presence of protozoa and epicomensais gregarines were observed in the shrimp examined. Infectious diseases affecting the shrimp are complex events and the continuous and accurate records of the farms are essential for epidemiological studies. These, identifying risk factors and involving them in the analysis are essential to maintain and monitor the health of animals and for the good productivity of crops. Camarões cultivados podem ser acometidos tanto por enfermidades não infecciosas, causadas por agentes abióticos, quanto por enfermidades infecciosas, sendo dentre essas as de etiologia viral as responsáveis pelas maiores perdas na carcinicultura. Camarões pequenos e médios foram coletados de quatro fazendas comerciais no litoral do estado de Pernambuco, nos períodos chuvoso e de estio, com o objetivo de investigar a frequência de enfermidades nesses animais. A investigação das viroses Mionecrose Infecciosa Viral (IMNV), Necrose Hematopoiética e Hipodermal Infecciosa (IHHNV) e a Síndrome da Mancha Branca (WSSV) foi realizada pela análise macroscópica, por meio de exame a fresco, microscopicamente, por meio da histopatologia, e por análises moleculares (Reação em Cadeia da Polimerase – PCR e nested PCR), identificando a presença ou não dos agentes etiológicos. HHNV e IMNV foram detectados, assim como lesões sugestivas dessas viroses foram mais frequentes no período de estio. O WSSV não foi detectado por nested PCR nos espécimes. Também não foram verificadas lesões que permitissem a suspeita da circulação deste vírus no período do estudo.Lesões sugestivas de bacterioses, enterite hemocítica, assim como presença de protozoários epicomensais e gregarinas foram constatados nos camarões analisados. Enfermidades infecciosas que acometem camarões cultivados são eventos complexos e registros exatos e contínuos das fazendas são indispensáveis para os estudos epidemiológicos. Esses, identificando fatores de risco e associando-os nas análises são fundamentais para manter e monitorar a saúde dos animais e para a boa produtividade dos cultivos.

Submitted by (edna.saturno@ufrpe.br) on 2016-10-13T13:00:47Z No. of bitstreams: 1 Joao Menezes Guimaraes.pdf: 673478 bytes, checksum: ef54de7c4a3b2b028a8d0c3258c0eb0f (MD5) Made available in DSpace on 2016-10-13T13:00:47Z (GMT). No. of bitstreams: 1 Joao Menezes Guimaraes.pdf: 673478 bytes, checksum: ef54de7c4a3b2b028a8d0c3258c0eb0f (MD5) Previous issue date: 2011-02-11 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES The environmental changes during fish culture reflect directly in the animal health, so the physiologic state should be evaluated systematically. It’s important to assess the animal health during whole culture, making possible an intervention as fast as necessary when any anomaly be detected. The hematological exams are an important tools to diagnose early symptoms. The aim of this study was to investigate the hematological reference values for Nile tilapia, Oreochromis niloticus (Cichlidae), cultured in Pernambuco. Were analyzed 109 fishes with weight and length mean of 525g and 27.7cm. Were determined the hematocrit, red blood cells (RBC); white blood cells (WBC); total thrombocyte; differential count of leukocytes expressed in relative and absolute values; hemoglobin concentration; mean corpuscular volume (MCV); mean corpuscular hemoglobin concentration (MCHC) and mean corpuscular hemoglobin (MCH). When evaluated separately the fishes from two different groups according to the culture technique (pond or cage), great heterogeneity was observed between the two groups. The mean value of RBC from tilapia cultured in ponds was 2.14x106/μL and in cages was 1.86x106/μL, being around 15% greater. The mean value of hemoglobin and hematocrit were, respectively, 16.87% and 26.14% greater in the pond cultured fish. Was verified difference (P

Submitted by (edna.saturno@ufrpe.br) on 2017-02-15T16:28:45Z No. of bitstreams: 1 Luciano Wiladino Andrade de Oliveira.pdf: 914370 bytes, checksum: a14bfcc0e2de00d3cc6e66654da9524c (MD5) Made available in DSpace on 2017-02-15T16:28:45Z (GMT). No. of bitstreams: 1 Luciano Wiladino Andrade de Oliveira.pdf: 914370 bytes, checksum: a14bfcc0e2de00d3cc6e66654da9524c (MD5) Previous issue date: 2010-08-24 The main bottleneck for seahorse production is the low survival of the juveniles during the early stages of development. Although the causes for low survival are not totally understood, feeding is considered critical as success on marine fish larval breeding is dependent, among others factors, on the size and nutritional content of the prey organisms. This study evaluated the growth and survival of newborn seahorses Hippocampus reidi fed on different preys. The experiment was conducted on 38 liter aquaria connected to a seawater recirculation system, which contained mechanical and biological filters, UV sterilizer and protein skimmer. Each aquarium had two aeration points near the water surface. Six treatments were tested: Art00h – newly hatched Artemia nauplii (5 ind ml-1); Art24h – 24 hours enriched Artemia metanauplii (5 ind ml- 1); Rot+Art24h - Brachionus plicatilis rotifers (10 ind ml-1) from day 1 until day 7 followed by enriched Artemia metanauplii (5 ind ml-1); Cop – Tisbe biminiensis (2 ind ml-1); and Cop+Art24h – mixed diet with T. biminiensis (2 ind ml-1) and 24 hours enriched Artemia metanauplii (5 ind ml-1). In the treatment Starvation, no food item was provided. All the treatments, except the starvation, received microalgae Nannochloropsis oculata every other day at concentrations that ranged from 2.2 x 105 to 3.2 x 106. Each experimental unit received 100 newborn seahorses. At the end of the experiment, all remaining seahorses were measured and weighed. Treatment Cop+Art24h resulted in a significantly higher survival (33.5 ± 5.4%), followed by Cop (6.6 ± 4.8%), Art00h (6.0 ± 8.3%) and Rot+Art24h (0.3 ± 0.5%), which were not significantly different. No survivors were observed in the remaining treatments. Growth parameters were significantly higher in Cop+Art24h and Art00h. The results from this study suggest that the feeding copepods T. biminiensis increases growth and survival of the seahorse H. reidi during the first two weeks of life. Um dos principais limitantes para o sucesso na produção de cavalos-marinhos em cativeiro é a baixa sobrevivência dos juvenis nos primeiros dias de vida. Embora as causas dessa baixa sobrevivência não sejam totalmente entendidas, a alimentação é considerada crucial para a diminuição da mortalidade, uma vez que o sucesso do cultivo de larvas e juvenis de peixes marinhos depende, entre outros fatores, do conteúdo nutricional e do tamanho do alimento ofertado. O objetivo deste estudo foi avaliar o efeito de diferentes protocolos de alimentação e manejo no crescimento e sobrevivência de juvenis recém nascidos do cavalo-marinho Hippocampus reidi até o 14o dia de vida. Foram utilizados aquários de 38 litros de água do mar integrados a um sistema de recirculação, o qual contava com filtros mecânicos, filtro de radiação ultravioleta, filtro biológico e um fracionador de proteína (“skimmer”). Cada aquário possuía dois pontos de aeração próximos à superfície da água. O experimento contou com seis tratamentos: Art00h - Artemia recém eclodida (5 ind ml-1); Art24h - Artemia enriquecida por 24 horas com a emulsão DHA Selco® (5 ind ml-1); Rot+Art24h - rotíferos Brachionus plicatilis (10 ind ml-1) até o setimo dia, seguido de Artemia enriquecida (5 ind ml-1); Cop – naúplios e copepoditos de Tisbe biminiensis (2 ind ml-1); e Cop+Art24h - dieta mista de copépodo T. biminiensis (2 ind ml-1) mais Artemia enriquecida (3 ind ml-1). No sexto tratamento (Jejum) não foi fornecido nenhum alimento. Todos os tratamentos, exceto o jejum, tiveram a adição da microalga Nannochloropsis oculata a cada dois dias em concentrações entre 2,2 x 105 até 3,2 x 106 células por ml. Foram realizadas quatro repetições por tratamento com 100 indivíduos por unidade experimental. Ao final do experimento, os indivíduos sobreviventes foram pesados e medidos (comprimento padrão, altura e peso). O tratamento Cop+Art24h apresentou sobrevivência significativamente maior (33,5 ± 5,4%), seguido pelos tratamentos Cop (6,6 ± 4,8%), Art00h (6,0 ± 8,3%) e Rot+Art24h (0,3 ± 0,5%), que não diferenciaram entre si. Nos demais tratamentos houve mortalidade total. Os parâmetros de crescimento foram significantemente maiores nos tratamentos Cop+Art24h e Art00h. Os resultados deste experimento sugerem que os copépodos T. biminiensis são um bom complemento na alimentação de juvenis do cavalo-marinho H. reidi.

Submitted by (edna.saturno@ufrpe.br) on 2017-02-07T11:34:15Z No. of bitstreams: 1 Alexandre Duarte Rodrigues da Silva.pdf: 439100 bytes, checksum: 1b2fd1350f22fda3959acac92bbccf28 (MD5) Made available in DSpace on 2017-02-07T11:34:15Z (GMT). No. of bitstreams: 1 Alexandre Duarte Rodrigues da Silva.pdf: 439100 bytes, checksum: 1b2fd1350f22fda3959acac92bbccf28 (MD5) Previous issue date: 2010-06-14 Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPq Culture of Litopenaeus vannamei in freshwater (0.0 g L-1) and low alkalinity and hardness (

Submitted by (edna.saturno@ufrpe.br) on 2016-10-07T13:54:35Z No. of bitstreams: 1 Fernanda Silva de Meirelles.pdf: 599414 bytes, checksum: be09ab548e388263d8d38ab7cabb0cf5 (MD5) Made available in DSpace on 2016-10-07T13:54:35Z (GMT). No. of bitstreams: 1 Fernanda Silva de Meirelles.pdf: 599414 bytes, checksum: be09ab548e388263d8d38ab7cabb0cf5 (MD5) Previous issue date: 2010-02-26 Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPq In Brazil, it is considered that the annual production of tilapias overcomes 100 thousand tons, mainly due to the productive potential of the Northeast area that answered for 41% of the total production of 70.000 tons in 2004, generating income of US$ 200.000.000,00, due to your climatic conditions, technology readiness and consumption market, regional and national in expansion. The intensification of cultivations has as consequence the increase of organic matter, that it favors the multiplication of microorganisms, making possible him/it supplies of diseases in the occurrence of adverse situations to the fish. Tilapia (Oerochromis niloticus) of cultivations of the state of Pernambuco they were appraised with objective of determining the frequency of bacterial diseases. For so much, samples of several farms were analyzed, in the several aspects, with relevance for the bacterial agents. The tilapia were examined with relationship to the streptococcus presence, víbrios, coliform, pseudomonas and aeromonas. Clinical exams and autopsy were accomplished, for microscopic analysis of the lesions and better definition of the probable agent etiological. The analyses were accomplished at the Laboratory of Sanity of Aquatic Animals (LASAq) of the Rural Federal University of Pernambuco (UFRPE) and in the Center of Development and Diffusion of Technology in Aquaculture of the State University of Bahia (UNEB). The principal pathological agents were víbrios and aeromonas, that are pathogens responsible opportunists for significant losses, being identified the following species in 46 isolated: V. natriegens (1/46), V. metschnikovii (2/46), V. halioticoli (1/46), V. fischeri (2/46), V. mimicus (23/46), V. diabolicus (1/46), V. furnissi (1/46), V. cholerae o1 (1/46), V. scophthalmi (4/46), V. proteolyticus (3/46), V. argarivorans (1/46), V. ordalii (2/46) and Vibrio spp. ( 3/46). The isolated vibrios presented larger sensibility to the antimicrobianos enrofloxacina (100%) and florfenicol (98,18%) and in decreasing order, gentamicina (90,91%), cotrimoxazol (sulfametoxazol+trimetroprim) (76,36%), tetraciclin (67,27%), eritromicin (30,91%) and amoxilina (3,64%). The isolated ones tested they came 100% resistant the penicillin. In this study, 96,4% (53/55) of the víbrios they presented index multiple resistance to antibiotics (MAR), superior to 0,22, characterizing multiple resistance. In 35 isolated the identified aeromonas were: A.caviae (1/35), A. shubertii (11/35), A. media (4/35), A. popoffii (1/35), A. sobria (3/35), A. encheleia (4/35), A. veronii (4/35) e A. jandaei (4/35) Forty were tested isolated of aeromonas with relationship to the sensibility to 08 antimicrobial, of these they showed larger sensibility the florfenicol (100,0%), enrofloxacina(95,0%), gentamicina (95,0%) and in decreasing order, cotrimoxazol (sulfametoxazol+trimetroprima) (67,5%), tetraciclina (65,0%). There was smaller sensibility the eritromicina (20,0%), penicillin (5,0%) and the amoxilina (2,5%), confirming the resistance existence. The index of multiple resistance to antibiotics (MAR) for the isolated of aeromonas it varied in an interval from 0,12 to 0,62, of these 95% (38/40) they came superior to 0,22 characterizing multiple resistance. Most of the species of isolated víbrios is not considered pathogenic for the fish (environmental), but nevertheless they can represent risk for the health of the tilapia for subject of opportunism and of the consumer, mainly if consumed raw. The isolated and identified movable aeromonas in this study are commonly considered as environmental, however many of the analyzed fish presented compatible symptomatology with the illness caused by these agents opportunists. No Brasil, estima-se que a produção anual de tilápias supere 100 mil toneladas, principalmente devido ao potencial produtivo da região Nordeste que em 2004 respondeu por 41 % da produção total de 70.000 toneladas, gerando renda de US$ 200.000.000,00, decorrente de suas condições climáticas, disponibilidade de tecnologia e mercado de consumo, regional e nacional em expansão. A intensificação de cultivos tem como conseqüência o aumento de matéria orgânica, que favorece a multiplicação de microrganismos, possibilitando o surto de doenças na ocorrência de situações adversas aos peixes. Tilápias (Oerochromis niloticus) de cultivos do estado de Pernambuco foram avaliadas com objetivo de determinar a freqüência de doenças bacterianas. Para tanto, analisaram-se amostras de diversas fazendas, nos diversos aspectos, com relevância para os agentes bacterianos. As tilápias foram examinadas quanto à presença de estreptococos, víbrios, coliformes, pseudomonas e aeromonas. Foram realizados exames clínicos e necropsias, para análise microscópica das lesões e melhor definição do provável agente etiológico. As análises foram realizadas no Laboratório de Sanidade de Animais Aquáticos (LASAq) da Universidade Federal Rural de Pernambuco (UFRPE) e no Centro de Desenvolvimento e Difusão de Tecnologia em Aqüicultura da Universidade Estadual da Bahia (UNEB). Os principais agentes patológicos foram víbrios e aeromonas, que são patógenos oportunistas responsáveis por perdas significantes, sendo identificadas as seguintes espécies em 46 isolados: Vibrio natriegens (1/46), V. metschnikovii (2/46), V. halioticoli (1/46), V. fischeri (2/46), V. mimicus (23/46), V. diabolicus (1/46), V. furnissi (1/46), V. cholerae O1 (1/46), V. scophthalmi (4/46), V. proteolyticus (3/46), V. argarivorans (1/46), V. ordalii (2/46) e Vibrio spp.(3/46). Os vibrios isolados apresentaram maior sensibilidade aos antimicrobianos enrofloxacina (100%) e florfenicol (98,18%) e em ordem decrescente, gentamicina (90,91%), cotrimoxazol (sulfametoxazol+trimetroprima) (76,36%), tetraciclina (67,27%), eritromicina (30,91%) e amoxilina (3,64%). Os isolados testados apresentaram-se 100% resistentes a penicilina. Neste estudo, 96,4% (53/55) dos víbrios apresentaram índice múltipla resistência a antibióticos (MAR), superior a 0,22, caracterizando múltipla resistência. Em 35 isolados as aeromonas identificadas foram: Aeromonas caviae (1/35), A. shubertii (11/35), A. media (4/35), A. popoffii (1/35), A. sobria (3/35), A. encheleia (4/35), A. veronii (4/35) e A. jandaei (4/35). Foram testados 40 isolados de aeromonas quanto à sensibilidade a oito antimicrobianos, tendo mostrado maior sensibilidade a florfenicol (100,0%), enrofloxacina(95,0%), gentamicina (95,0%), cotrimoxazol (sulfametoxazol+trimetroprima) (67,5%), tetraciclina (65,0%). Houve menor sensibilidade a eritromicina (20,0%), penicilina (5,0%) e a amoxilina (2,5%), confirmando a existência de resistência. O índice MAR para os isolados de aeromonas variou de 0,12 a 0,62, dos quais 95% (38/40) apresentaram índices superiores a 0,22, caracterizando multiresistência. A maioria das espécies de víbrios isoladas não é considerada patogênica para os peixes (ambientais), mas ainda assim podem representar risco para a saúde das tilápias por questão de oportunismo e do consumidor, principalmente se consumido cru. As aeromonas móveis isoladas e identificadas neste estudo são comumente consideradas como ambientais, porém muitos dos peixes analisados apresentaram sintomatologia compatível com a enfermidade causada por estes agentes oportunistas.

Submitted by (ana.araujo@ufrpe.br) on 2016-08-01T17:35:24Z No. of bitstreams: 1 Kleber Napoleao Nunes de Oliveira Barros.pdf: 2964667 bytes, checksum: a3c757cb7ed16fc9c38b7834b6e0fa29 (MD5) Made available in DSpace on 2016-08-01T17:35:24Z (GMT). No. of bitstreams: 1 Kleber Napoleao Nunes de Oliveira Barros.pdf: 2964667 bytes, checksum: a3c757cb7ed16fc9c38b7834b6e0fa29 (MD5) Previous issue date: 2010-02-22 It is presented in this work the growth model nonlinear Chapman-Richards with distribution of errors following the new class of symmetric models processed and Bayesian inference for the parameters. The objective was to apply this structure, via Metropolis-Hastings algorithm, in order to select the equation that best predicted heights of clones of Eucalyptus urophilla experiment established at the Agronomic Institute of Pernambuco (IPA) in the city of Araripina . The Gypsum Pole of Araripe is an industrial zone, located on the upper interior of Pernambuco, which consumes large amount of wood from native vegetation (caatinga) for calcination of gypsum. In this scenario, there is great need for a solution, economically and environmentally feasible that allows minimizing the pressure on native vegetation. The generus Eucalyptus presents itself as an alternative for rapid development and versatility. The height has proven to be an important factor in prognosis of productivity and selection of clones best adapted. One of the main growth curves, is the Chapman-Richards model with normal distribution for errors. However, some alternatives have been proposed in order to reduce the influence of atypical observations generated by this model. The data were taken from a plantation, with 72 months. Were performed inferences and diagnostics for processed and unprocessed model with many distributions symmetric. After selecting the best equation, was shown some convergence of graphics and other parameters that show the fit to the data model transformed symmetric Student’s t with 5 degrees of freedom in the parameters using Bayesian inference. É abordado neste trabalho o modelo de crescimento não linear de Chapman-Richards com distribuição dos erros seguindo a nova classe de modelos simétricos transformados e inferência Bayesiana para os parâmetros. O objetivo foi aplicar essa estrutura, via algoritmo de Metropolis-Hastings, afim de selecionar a equação que melhor estimasse as alturas de clones de Eucalyptus urophilla provenientes de experimento implantado no Instituto Agronômico de Pernambuco (IPA), na cidade de Araripina. O Polo Gesseiro do Araripe é uma zona industrial, situada no alto sertão pernambucano, que consume grande quantidade de lenha proveniente da vegetação nativa (caatinga) para calcinação da gipsita. Nesse cenário, há grande necessidade de uma solução, econômica e ambientalmente, viável que possibilite uma minimização da pressão sobre a flora nativa. O gênero Eucalyptus se apresenta como alternativa, pelo seu rápido desenvolvimento e versatilidade. A altura tem se revelado fator importante na prognose de produtividade e seleção de clones melhores adaptados. Uma das principais curvas de crescimento, é o modelo de Chapman- Richards com distribuição normal para os erros. No entanto, algumas alternativas tem sido propostas afim de reduzir a influência de observações atípicas geradas por este modelo. Os dados foram retirados de uma plantação, com 72 meses. Foram realizadas as inferências e diagnósticos para modelo transformado e não transformado com diversas distribuições simétricas. Após a seleção da melhor equação, foram mostrados alguns gráficos da convergência dos parâmetros e outros que comprovam o ajuste aos dados do modelo simétrico transformado t de Student com 5 graus de liberdade utilizando inferência Bayesiana nos parâmetros.

Submitted by (edna.saturno@ufrpe.br) on 2017-02-07T12:40:31Z No. of bitstreams: 1 Ana Lia Camposa Quaggio.pdf: 1430515 bytes, checksum: 68e33f51877fa5eba2d7e0997b3e6520 (MD5) Made available in DSpace on 2017-02-07T12:40:31Z (GMT). No. of bitstreams: 1 Ana Lia Camposa Quaggio.pdf: 1430515 bytes, checksum: 68e33f51877fa5eba2d7e0997b3e6520 (MD5) Previous issue date: 2010-02-08 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES Since the 50’s, Xiphias gladius (Linnaeus, 1758) has been captured along the Brazilian coast by surface longliners. This is the second most important target species for the tuna fleet based in Itajaí, Santa Catarina State, after the blue-shark, Prionace glauca (Linnaeus, 1758). The study was based on data from sea observers, log books and carcass ladings of Xiphias gladius, Linnaeus, 1758) from the pelagic longline fleet based in Itajaí, which operated in the southwest Atlantic. Covariance models were fit to the catches (weight, kg) of swordfish and the effects used were: (1) the years 1997, 1998, 2001, 2002 e 2007 (2) lunar phases and (3) seasons. The covariate was fishing effort (hooks). The highest moon catches occurred on the first quarter moon and during wintertime. The Mantel-Haenszel procedure was used to test the hook selectivity and showed that the type “J” hook (J 9/0 10º offset), traditionally used by the fleet, have 1.2 more chances of catching Xiphias gladius than the “circle” hook (18/0 10º offset), used as an optinal device to reduce sea turtle bycatch. The M-H test showed that the J hook has 1,2 more chances of catching Xiphias gladius than the circle hook. The conversion of individual carcass weights (kg) to total length (cm) for the years 2000 to 2002 showed that 66% of the catches were sub-adults. O espadarte, Xiphias gladius (Linnaeus, 1758), vem sendo capturado na costa brasileira desde os anos 50 pelo espinhel-de-superfície. Esta é a segunda mais importante espécie-alvo da frota atuneira sediada em Itajaí (SC), ficando atrás apenas do tubarão-azul, Prionace glauca (Linnaeus, 1758). No presente estudo foram analisados os dados obtidos para esta espécie através de observadores, mapas de bordo e fichas de desembarque de carcaças de X. gladius para a frota de espinhel-de-superfície sediada em Itajaí (SC) e atuante no Atlântico sudoeste. Foram elaborados modelos de análise de covariância (ANCOVA) e aplicados aos dados de captura (peso em kg) do espadarte, tendo como efeitos: (1) os anos de 1997, 1998, 2001, 2002 e 2007 (2) as fases lunares e (3) as épocas do ano. A covariável foi o esforço de pesca (n° de anzóis). As maiores médias de captura ocorreram durante as fases de lua crescente e no inverno. O procedimento estatístico de Mantel-Haenszel teste foi utilizado para comparar as capturas do espadarte no anzol tipo “Jota” (9/0 10º offset) comumente utilizado pela frota espinheleira com o anzol tipo “circular” (18/0 10ºoffset), este último oferecido como alternativa mitigadora à captura de tartarugas marinhas. O teste M-H indicou que o anzol “Jota” apresentou 1,2 mais chances de capturar Xiphias gladius que no “circular”. A conversão dos pesos individuais das carcaças (kg) em comprimentos totais (cm), referentes aos anos de 2000 a 2002, indicou que 66% das capturas foram de sub-adultos, ao longo de todo o ano.

Submitted by (ana.araujo@ufrpe.br) on 2016-05-25T15:49:21Z No. of bitstreams: 1 Carlos Sergio Araujo dos Santos.pdf: 1655656 bytes, checksum: 7687ae013a71aadd4e9e9bf4783a172f (MD5) Made available in DSpace on 2016-05-25T15:49:21Z (GMT). No. of bitstreams: 1 Carlos Sergio Araujo dos Santos.pdf: 1655656 bytes, checksum: 7687ae013a71aadd4e9e9bf4783a172f (MD5) Previous issue date: 2010-01-15 Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPq Box and Cox (1964) developed a numerical procedure to transform the response variable such that the transformed variable should be as closed as possible to the normal distribution. The introduction of a new class of non linear symetric transformed models aims to extended the Box and Cox models to a general class of symetric models. The new class of models inclued all the continuos symmetric distributions with a possible non linear structure to the mean, making possible the use of the new class of regression models. It was applied in the estimate of volumes of the Eucalyptus tereticornis clones, with 7,5 years, planted in the Experimental Station of Araripe of the Agronomic Institute of Pernambuco (IPA), in the municipality of Araripina, in the semiarid of Pernambuco. The non linear model used as pattern was the Schumacher and Hall model. The results indicates that the transformed model with t-Student erros with two degrees of freedon adjusted better to the data set. Box e Cox (1964) desenvolveram um procedimento numérico para escolher uma transformação da resposta tal que a distribuição da variável transformada esteja o mais próximo possível da distribuição normal. A introdução de uma nova classe de modelos simétricos transformados não lineares visa estender os modelos de Box e Cox para uma classe geral dos modelos simétricos. Esta nova classe de modelos inclui todas as distribuições contínuas simétricas com uma possível estrutura não linear para a média e capacitando o ajustamento de uma larga extensão de modelos para vários tipos de dados. Para ilustrar a utilidade dessa nova classe de modelos de regressão foi realizada uma aplicação na estimativa dos volumes de clones de Eucalyptus tereticornis com 7,5 anos oriundos de um experimento que está sendo realizado no Campo Experimental do Araripe do Instituto Agronômico de Pernambuco (IPA), localizado no Município de Araripina, no semiárido Pernambucano. O modelo não-linear utilizado para explicar os dados foi o modelo Schumacher-Hall. Diante dos resultados obtidos se concluí que o modelo transformado com erros t-Student com dois graus de liberdade foi o que melhor se ajustou os dados.

Submitted by (edna.saturno@ufrpe.br) on 2017-02-22T16:50:48Z No. of bitstreams: 1 Virgina Fonseca Pedrosa.pdf: 303865 bytes, checksum: 420df1fdf37bd4dee6c57213ba1e8f55 (MD5) Made available in DSpace on 2017-02-22T16:50:48Z (GMT). No. of bitstreams: 1 Virgina Fonseca Pedrosa.pdf: 303865 bytes, checksum: 420df1fdf37bd4dee6c57213ba1e8f55 (MD5) Previous issue date: 2009-08-27 Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPq The use of methods for identification of agents etiológicos represents an important tool to diagnose illnesses and consequently, to try to minimize the losses caused by the agent's permanence in the fish farmings. In this sense, it was aimed at to associate the lesions anatomopathological with the diversity of bacterias found in collected tilápias of different cultivation systems in Pernambuco. 58 tilápias samples were collected in the summertime period and rainy, that were submitted to analyses anatomopathological and bacteriological, having been observed the occurrence of Aeromonas spp. (29,3%), Vibrio spp. (41,4%) and enterobactérias of the goods Klebsiella spp. (17,2%), Leminorella spp. (1,7%), Obesumbacterium spp. (5,2%), Proteus spp. (1,7%), Providencia spp. (19%), Salmonella spp. (1,7%), Shigella spp. (1,7%) and Taturnella spp. (14%). A significant dependence was detected (p

Submitted by (edna.saturno@ufrpe.br) on 2017-02-20T13:40:05Z No. of bitstreams: 1 Rodrigo Risi Pereira Barreto.pdf: 2720946 bytes, checksum: f60d89c3648bb992cd8d11c36c037cd5 (MD5) Made available in DSpace on 2017-02-20T13:40:05Z (GMT). No. of bitstreams: 1 Rodrigo Risi Pereira Barreto.pdf: 2720946 bytes, checksum: f60d89c3648bb992cd8d11c36c037cd5 (MD5) Previous issue date: 2009-05-01 Between 1994 and 2008, 369 blacknose sharks, Carcharhinus acronotus (43,5-146 cm total length, TL) were collected from gill nets and surveys carried out on Pernambuco’s continental shelf, Brazil (07° 15’ 45’’ S and 09° 28’ 18’’ S). Vertebral sections of 109 males (43,5-124 cm TL) and 141 females (46-146 cm TL) were analyzed; the index of average percentage of error (IAPE) ranged from 1:17 to 13:33% (mean 6,13%) in vertebrae displaying between 0 to 15 growth rings (GR). The marginal increment relative analysis (MIR) was not conclusive showing only a tendency of ring deposition between autumn and winter months. Significant differences were not found between the individual length (TL, cm) and the vertebra radius (VR, mm). Growth parameters were derived using von Bertalanffy function (VBGF a), von Bertalanffy modified (VBGF b), Gompertz and Schnute models. The von Bertalanffy growth model was that which best represented growth for the species. Parameters estimated for both sexes were: k = 0.12, t0 = -3.66, L¥= 142.06 cm. Male and female maturity was reached at 105 cm TL, presenting about 5 growth rings (GR's). The largest specimen whose age was observed had 15 growth rings (132 cm TL). Adults were only caught in longlines. Mortality was estimated from the biological information of the species according literarature. Mortality values obtained were: M = 0,275; Z = 0,709 (gillnets), 0,31 (longline); F = 0,43 (gillnets) 0,04 (longline) Z0 = 0,06 and Z' = 0,349. The Exploitation rates calculated for gillnets were 0,61 and 0,130 respectively. Considering a scenario of equilibrium, the population would increase by approximately 8,3% per 8,5 years (R0 = 1,971; G = 8,43; r = 0,080). However, taking into account the current level of exploitation, the population appears to be over-exploited, decreasing 13,6% in 8,3 years (R0 = 0,3; G = 8,3; r = -0,041). Entre 1994 e 2008, foram capturados 369 indivíduos de Carcharhinus acronotus (43,5-146 cm de comprimento total, CT) oriundos da pesca com redes de emalhar e prospecções na plataforma continental do estado de Pernambuco, Brasil (07° 15’ 45’’ S e 09° 28’ 18’’ S). Secções vertebrais de 109 machos (43,5-124 cm CT) e 141 fêmeas (46-146 cm CT) foram analisadas. O índice do erro médio (IAPE) variou entre 1,17 a 13,33 % (média 6,13 %) entre 0-15 anéis etários. A análise de incremento marginal relativo (MIR) não foi conclusiva embora tenha apresentado uma tendência de formação do anel entre os meses do outono e inverno. Não foram detectadas diferenças significativas entre o comprimento individual (CT, cm) e o raio da vértebra (RV). Os parâmetros de crescimento foram estimados através da utilização dos modelos de von Bertalanffy (VBGF a), von Bertalanffy modificado, (VBGF b) Gompertz e Schnute. O modelo de von Bertalanffy foi o que melhor representou o crescimento para a espécie. Os parâmetros obtidos para os comprimentos observados para os sexos agrupados foram: L = 142,06 cm CT, k = 0,12, t0 = -3,66. Não foram detectadas diferenças significativas entre as curvas de crescimento para os sexos (p > 0,05). Machos e fêmeas maturam em torno dos 105 cm CT quando apresentam 5 anéis etários. O maior espécime cuja idade foi estimada foi uma fêmea que apresentou 15 anéis (132 cm CT). Adultos foram capturados somente no espinhel. As taxas de mortalidades foram determinadas a partir das informações disponíveis na literatura e dos parâmetros de crescimento obtidos. Os valores estimados por esses métodos indiretos foram: M = 0,275; Z = 0,709 (emalhe), 0,310 (espinhel), F = 0, 434 (emalhe) e 0, 044 (espinhel); Z0 = 0, 063 e Z’ = 0, 349. As taxas de exploração calculadas para o emalhe e o espinhel foram 0,61 e 0,130 respectivamente. Em cenário de equilíbrio a população teria condições de aumentar em aproximadamente 8,3 % a cada 8,5 anos (R0 =1, 971 G = 8,43; r = 0, 080). No entanto, considerando o nível de pesca atual, a população se apresenta sobre-explorada, diminuindo em 13,6 % a cada 8,3 anos (R0 =0,3 G = 8,23; r = -0, 041).

Submitted by (edna.saturno@ufrpe.br) on 2017-02-09T14:22:55Z No. of bitstreams: 1 Dijaci Araujo Ferrera.pdf: 375861 bytes, checksum: 44d93b1224eb4ddd72db039092eaa1de (MD5) Made available in DSpace on 2017-02-09T14:22:55Z (GMT). No. of bitstreams: 1 Dijaci Araujo Ferrera.pdf: 375861 bytes, checksum: 44d93b1224eb4ddd72db039092eaa1de (MD5) Previous issue date: 2009-04-17 Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPq Zero-water exchange systems and low salinity culture are used by shrimp farmers as alternatives to alleviate the restrictions imposed by environmental regulations regarding the use of coastal areas, and allow better control of diseases and their spread. Thus, the effects at six densities (2000, 4000, 6000, 8000, 10000, and 12000 shrimp/m²) in Litopenaeus vannamei reared under zero water exchange and low salinity were evaluated in a complete randomized design with three replications. Eighteen fiberglass tanks (1000 L) were stocked with postlarvae (PL18) at densities corresponding to each treatment and fed commercial diets. Daily, molasses was added to water for provide C/N ratio between 20 and 30:1, to encourage the development of heterotrophic bacterial community. The study showed that density increase influenced significantly (P

Submitted by (ana.araujo@ufrpe.br) on 2016-08-05T13:36:28Z No. of bitstreams: 1 Marcelle Marques da Silva.pdf: 960120 bytes, checksum: 2630be7e6c34db10ff7c5fedf307426e (MD5) Made available in DSpace on 2016-08-05T13:36:28Z (GMT). No. of bitstreams: 1 Marcelle Marques da Silva.pdf: 960120 bytes, checksum: 2630be7e6c34db10ff7c5fedf307426e (MD5) Previous issue date: 2009-02-19 The objective to maximize the survival of the post-larvae (PL) of the marine shrimp Litopenaeus vannamei being related with the variable of handling (density of stockage of the post-larvae, month of culture, days of culture, nurseries and laboratories supplying of the post-larvae) when cultivated in the nurseries. They had been 65 data of culture of a commercial farm located in the state of Rio Grande do Norte corresponding to the period from october 2004 to march 2007. The post-larvae (PL) were storage in nursery with densities varied from 12 to 64 PL/L in a period of five to 19 days. The system of culture adopted in the nursery phase was the intensive with capacity of 50.000 liters, with system of aeration and draining independents. The supplying of the tanks was made using water of the canal of supplying of the fisheries. The feeding used was to the base of biomass of artêmia and commercial ration with 40% of rude protein, and was managed of daily of two in two hours. To relate the survival of the post-larvae with the culture variable it was used multiple regression, where its parameters had been esteem on the base of the minimization the sum of square of residues. The selection of variable was carried through using the process of Stepwise Forward, establishing value 3 of statistics “F” of Snedecor of entrance and exit, associated the Montgomery and Peck to minimize the sum of square of residues. To verify the adequacy of the modelto the data, the linearity,normality and homogeneity for the residues had been the analysis of variance (ANOVA) and analysis residues. Thus, to the shape a survival of the post-larvae of the marine shrimp Litopenaeus vannamei in the nursery phase, with the variable of handling, it was gotten R2= 60,81%, and through the ANOVA is verified that the level of probability of rejection of the model was very small. It was concluded that we can shape the survival with the culture parameters, where the origin of the post-larvae, the time of culture and the density influence of significant form inthe survival and send regards to the producer that it uses PL of the laboratories LFPL2, LFPL3, LFPL4 e LFPL7, therefore with certainty it will get success in its production. Objetivou-se maximizar a sobrevivência das pós-larvas (PL) do camarão marinho Litopenaeus vannamei relacionando-a com as variáveis de manejo (densidade de estocagem das pós-larvas, mês de cultivo, tempo de cultivo, tanques berçários e laboratórios fornecedores das pós-larvas), quando cultivadas em tanques berçários. Foram utilizados 65 dados de cultivos de uma fazenda comercial, localizada no estado do Rio Grande do Norte, correspondente ao período de outubro de 2004 a março de 2007. As PL foram estocadas em tanques berçários às densidades que variaram de 12 a 64 PL/L, durante um período de cinco a 19 dias. O sistema de cultivo adotado foi o intensivo em tanques com capacidade de 50.000L, com sistema de aeração e drenagem independentes. O abastecimento dos tanques foi feito utilizando-se água do canal de abastecimento dos viveiros. A alimentação utilizada foi à base de biomassa de artêmia e ração comercial com 40% de proteína bruta, administrada diariamente de duas em duas horas. Para relacionar a sobrevivência das PL com as variáveis de cultivo utilizou-se a regressão múltipla, em que seus parâmetros foram estimados com base na minimização da soma dos quadrados. A seleção de variáveis foi realizada utilizando-se o processo StepwiseForward estabelecendo o valor três da estatística “F” de Snedecor de entrada (Fentrada) e de saída (Fsaída), associado com a técnica de Montgomery e Peck para minimizar a soma dos quadrados dos resíduos. Para verificar a adequação do modelo aos dados, a linearidade, normalidade e homogeneidade dos resíduos foram utilizados a análise de variância (ANOVA) e a análise de resíduos. Assim, ao modelar a sobrevivência (Sob-%) das PL com as variáveis de manejo, obteve-se R2= 60,81% e através da ANOVA verificou-se que o nível de probabilidade de rejeição do modelo foi muito pequeno. Verifica-se que podemos modelar a sobrevivência com os parâmetros de cultivo, onde a procedência das PL, o tempo de cultivo e a densidade influem de forma significativa na sobrevivência, e recomenda-se ao produtor que utilize PL dos laboratórios LFPL2, LFPL3, LFPL4 e LFPL7, pois com certeza ele obterá sucesso no seu agronegócio.

Submitted by (edna.saturno@ufrpe.br) on 2016-10-13T12:20:42Z No. of bitstreams: 1 Joanna Dourado.pdf: 721470 bytes, checksum: 1ade18b6e4af854fcb0abb1162f7c2bb (MD5) Made available in DSpace on 2016-10-13T12:20:42Z (GMT). No. of bitstreams: 1 Joanna Dourado.pdf: 721470 bytes, checksum: 1ade18b6e4af854fcb0abb1162f7c2bb (MD5) Previous issue date: 2009-02-13 Shrimp farming all over the world has been affected in the last years by many diseases, that have contributed to the development indexes decline in the production of farmed marine shrimp. On top of the losses due to diseases, it's important to mention that some of the vibrio species are zoonotic agents, meaning they can also cause diseases in humans. Given the importance of bacteria of the genus Vibrio to shrimp farming and public health, the aim of this study was to evaluate marine shrimp, correlate the clinical findings with the presence of the vibrio bacteria and identify the isolated species in the water of the ponds of cultivated marine shrimp in Pernambuco. The samples were collected from four coastal farms and the laboratory analysis consisted of the research and identification of the vibrio bacteria in the hepatopancreas and in the water of the tanks, and identification of any alteration by examination of fresh samples. Thiosulphate citrate bile salt agar (TCBS) was used as the culture media and the selected colonies were later biochemically tested. Four hundred and eighty shrimps were analyzed, of hose 424 (88.33%) showed to have vibrio and one or more alterations. There were identified a total of 903 alterations, the most frequent of muscle tissue (329; 36.43%) and the least frequent of the hepatopancreas tubules (121; 13.40%). When analizing the growth cycle, it was observed the largest number of alterations (503; 55.7%) in the dry season. Taking into account the weight of the shrimps, it was observed 540 (59.8%) alterations when the average weight was 8g. Out of all the analyzed animals, 314 (65.42%) showed to have vibrio, being 38 samples from the ponds water and 605 shrimp isolated. The most abundant species was V. mediterranei, (21.31%), considered an environmental species non pathogenic to shrimp, and the least abundant species were V. campbellii, V. rotiferanus, V. shiloni, V. splendidus, V. tapetis and V. wodanis (0.16 %). A dependency between the alterations found on the exam and the presence of the vibrio was noticed, showing that the aforementioned exam, combined to microbiological analyzes, is an important tool that should be used when monitoring the farms. It was also noticed a great variety of pathogenic and non pathogenic species of vibrio in the studied samples. Such microorganisms could, eventually, be related to shrimp and human infections, which could represent a risk for shrimp farming and public health respectively. A carcinicultura mundial tem enfrentado nos últimos anos vários impactos causados por enfermidades, que contribuíram para a queda dos índices de desenvolvimento na produção de camarão marinho cultivado. Além das perdas causadas devido às enfermidades, ressalta-se que algumas espécies de víbrio são agentes zoonóticos, ou seja, podem causar doenças também em humanos. Dada a importância das bactérias do gênero Vibrio para a carcinicultura e saúde pública, objetivou-se avaliar camarões marinhos, associar os achados clínicos com a presença de bactérias do gênero Vibrio e identificar as espécies isoladas da água dos viveiros e de camarões marinhos (Litopenaeus vannamei) cultivados no litoral do estado de Pernambuco. As amostras foram coletadas em quatro carciniculturas do litoral e as análises laboratoriais compreenderam pesquisa e identificação de bactérias do gênero Víbrio no hepatopâncreas e na água dos viveiros, e identificação de alterações através do exame a fresco. Para o isolamento das espécies foi utilizado o meio de cultivo Ágar Tiossulfato Citrato Sais de Bile Sacarose (TCBS) e as colônias selecionadas foram posteriormente submetidas a provas bioquímicas. Foram analisados no total 480 camarões, destes 424 (88,33%) apresentaram um ou mais tipos de alteração. Foi identificado um total de 903 alterações, sendo a mais freqüente a da musculatura (329; 36,43%) e a menos freqüente a alteração dos túbulos do hepatopâncreas (121; 13,40%). Com relação ao ciclo de cultivo, foi observado o maior número de alterações (503; 55,7%) no período de estio. Levando-se em consideração a média de peso dos camarões verificou-se 540 (59,8%) alterações quando os mesmos estavam com peso médio de 8 g. Do total de animais analisados 314 (65,42%) apresentavam vibrio e uma ou mais alteração no exame a fresco. Foi obtido um total de 643 isolados de Vibrio, sendo 38 de amostras de água dos viveiros e 605 isolados de camarão. A espécie mais abundante foi V. mediterranei (21,31%), considerada espécie ambiental e não patogênica para o camarão, e as espécies de menor predominância foram V. campbellii, V. rotiferianus, V. shiloni, V. splendidus, V. tapetis e V. wodanis (0,16%). Foi verificada uma dependência entre as alterações encontradas no exame a fresco e a presença de vibrio, concluindo-se que o referido exame, associado à análise microbiológica é uma importante ferramenta que deve ser utilizada no monitoramento da saúde dos animais. Verificou-se também uma ampla variedade de espécies patogênicas e não patogênicas de víbrio nas amostras estudadas, que podem eventualmente estar relacionados a infecções nos camarões e nos humanos, representando um risco para a carcinicultura e para a saúde pública respectivamente.

Submitted by (edna.saturno@ufrpe.br) on 2017-02-10T11:39:28Z No. of bitstreams: 1 Elaine Cristina Batista dos Santos.pdf: 495925 bytes, checksum: af23f3fff2e3cde71c7dbec53715876f (MD5) Made available in DSpace on 2017-02-10T11:39:28Z (GMT). No. of bitstreams: 1 Elaine Cristina Batista dos Santos.pdf: 495925 bytes, checksum: af23f3fff2e3cde71c7dbec53715876f (MD5) Previous issue date: 2009-02-10 The techniques of direct (Pdir) and indirect (Pind) settlement employed in the growthout of marine shrimp Litopenaeus vannamei, were analyzed, in order to determine their efficiency in the productive system of agribusiness. Data of 270 cultures from a commercial shrimp farm located in Rio Grande do Norte, Brazil, were used to relate the variables of management: time of culture (TC), stocking density (DE), pond area (VA), month of settlement (MP), year of settlement (AP), supplier of laboratory post larva (LFPL), direct settlement (Pdir) and indirect settlement (Pind), through the technique of multiple linear regression to estimate the parameters that influenced the cultures , with varying responses: production (PRD) productivity (PRDT), feed conversion factor (FCA), final weight (PMF) and survival rate (TS). These variables were related in terms of stocking density (DE) and days of culture (DC). For variables, PMF and FCA, the settlement form had not any influence (P ≥ 0.05). The other variables there was significant difference (P

Submitted by (edna.saturno@ufrpe.br) on 2016-08-01T15:49:41Z No. of bitstreams: 1 Andrea Barretto.pdf: 465481 bytes, checksum: 80d81c9df4236400373841c9117f86d9 (MD5) Made available in DSpace on 2016-08-01T15:49:41Z (GMT). No. of bitstreams: 1 Andrea Barretto.pdf: 465481 bytes, checksum: 80d81c9df4236400373841c9117f86d9 (MD5) Previous issue date: 2009-02-02 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES The study of blood cells, also known as hematology, has become for aquaculture a valuable tool in understanding the physiological changes that occur in organisms, however the shrimp, there is still much to discover about if the hemolymph. Considering the above objective of this work: to evaluate the effectiveness of the anticoagulant sodium citrate (10%) in blood tests, to check the influence of cooling on the total count of hemocytes (CTH), haematological data correlate with the presence of Vibrio in the hemolymph, to identify the influence of age and seasonality in the total count of haemocytes in shrimp Litopenaeus vannamei. The species were caught shrimp Litopenaeus vannamei from two nurseries of land (A and B) of mariculture Santa Cruz, located in the city of Goiana-PE. The collections are made weekly, for two cycles of cultivation (dry season and rainy season). It is the examination fresh (macro and microscopic) in all animals sampled. The total counts of hemocytes (cells/mm3) were performed immediately after collection and after five hours of chilling in a Neubauer chamber. Immediately after the counting, the samples intended for bacteriological analysis were transported in temperature to the laboratory of health of aquatic animals (LASAq), Universidade Federal Rural de Pernambuco. The data concerning the influence of cooling on the hemocytes were analyzed using the techniques of mathematical modeling (P

Submitted by (edna.saturno@ufrpe.br) on 2017-02-16T15:07:14Z No. of bitstreams: 1 Mauricio Nogueira da Cruz Pessoa.pdf: 716355 bytes, checksum: 41120b87fa94d8d978b32032793ede5f (MD5) Made available in DSpace on 2017-02-16T15:07:14Z (GMT). No. of bitstreams: 1 Mauricio Nogueira da Cruz Pessoa.pdf: 716355 bytes, checksum: 41120b87fa94d8d978b32032793ede5f (MD5) Previous issue date: 2008-10-01 The statistical evaluation of the use of extruded and pelleted ration in the Litopenaeus vannamei shrimp culture, under field conditions, were done with the objective of determinie the advantages of these diets on shrimp performance index. To perform the analyses were used 481 crops, from 8 commercial farms of northeastern Brazil. It was the technique used multiple linear regression to determine the parameters of handling variables that influenced the responses of crops such as production, productivity, the conversion factor of food (FCA), the weekly average growth, the average weight end, the quantity of feed consumed and survival. These responses were measured by the density of storage, but also in time of cultivation. It may be noted that there was no significant difference (P ≥ 0,05) between the use of feed on the variables average weight final and average weekly growth, as in other variables can be observed that for the storage density of 15 shrimps.m-2 there were differences of up to 20.1% in the FCA in favour of extruded ration, a production 9.7% higher when using pelleted ration of feed consumption and a 33.2% higher in pelleted feed. For densities of 60 shrimps.m-2 the difference in the FCA was 18.8% lower when you used to feed pelleted, production was 7.2% higher if using pelleted diet, consumption of feed was 8.5% lower when used if pelleted diet and productivity was 10.3% higher when the extruded feed was used for that density. Avaliações estatísticas do uso de ração extrusada e peletizada, em cultivos do camarão Litopenaeus vannamei, nas condições de campo, foram realizadas com o objetivo de determinar as vantagens sobre os índices zootécnicos dos camarões. Análises foram feitas utilizando-se 481 cultivos, provenientes de oito fazendas comerciais do nordeste do Brasil. Foi utilizada a técnica de regressão linear múltipla para estimar os parâmetros de manejo que influenciaram nas variáveis respostas dos cultivos tais como produção, produtividade, fator de conversão alimentar (FCA), crescimento semanal, peso final, quantidade de ração administrada e sobrevivência. Essas variáveis foram avaliadas em função da densidade de estocagem e do tempo de cultivo. Não houve diferença significativa (P ≥ 0,05) entre o uso das rações sobre as variáveis, peso final e crescimento semanal. Nas demais variáveis observou-se que na densidade de estocagem de 15 camarões/m2 houve diferença de até 20,1% no FCA a favor da ração extrusada, uma produção 9,7% maior quando se utilizou ração peletizada e um consumo a mais de 33,2% de ração peletizada. Para densidades de 60 camarões/m2 a diferença no FCA foi de 18,8% menor quando se utilizou ração peletizada, a produção foi 7,2% maior utilizando-se ração peletizada, o consumo de ração foi 8,5% menor com ração peletizada e a produtividade foi 10,3% maior quando a ração extrusada foi utilizada.

Submitted by (edna.saturno@ufrpe.br) on 2017-02-21T16:48:08Z No. of bitstreams: 1 Samia Regia Rocha Monteiro.pdf: 361943 bytes, checksum: 282e86342e0a7dc7cefcb1a57d78fd60 (MD5) Made available in DSpace on 2017-02-21T16:48:08Z (GMT). No. of bitstreams: 1 Samia Regia Rocha Monteiro.pdf: 361943 bytes, checksum: 282e86342e0a7dc7cefcb1a57d78fd60 (MD5) Previous issue date: 2008-08-29 The use of artificial substrates in aquaculture has been used to provide the increase of culture area and natural food, improving the water quality of shrimp performance. In order to maximize the survival, feed conversion and final weight of marine shrimp Litopenaeus vannamei, cultured in oligohaline water (>0.5‰) and in heterotrophic system, were evaluated the effect of adding screens as artificial substrate. Post-larvae with 10 days (PL10) were acclimated from 34‰ to 0.5‰, during 12 days. After of the acclimatization process, the postlarvae (PL22) were reared to obtain 0.3±0.1g of weight and then they were transferred to 18 fiberglass tanks (800 L of net volume) and stocked with 150 PL/tanque. The substrate percentage addition (SPA), represents 100, 150, 200, 250 and 300% of bottom area (1m2) and the control “0” SPA, it was evaluated in completely randomized design, with three replicates. During the culture, were used a 40% crude protein (CP) ration during the first 30 days, andanother with 35% CP during the a 15 days, offered daily at 8, 12 and 17 hrs. After 45 days of culture the survival ranged from 45 to 84%, between treatments with 300% and 100% of substrate, respectively. The shrimp final weight, when related to the length orbit and the time of culture, did not present statistical difference between the SPA (P>0.05). In the ratio length and culture time, the treatment 300% was statistical different in the SPA-0, 200 and 250%. In conclusion, the use of artificial substrate increased the variables weight gain, feed conversion ratio, growth rate and survival when using SPA between 87.8 and 166.7%. O uso de substratos artificiais na aqüicultura vem sendo utilizado para disponibilizar maior área de cultivo e alimento natural, melhorando a qualidade de água e desempenho do camarão. Objetivando maximizar a sobrevivência, conversão alimentar e peso final do camarão marinho Litopenaeus vannamei, quando cultivado em água oligohalina (>0,5‰) e em meio heterotrófico, foram avaliadas a influência da adição de telas como substrato artificial. Pós-larvas com 10 dias (PL10) foram aclimatadas de 34‰ para 0,5‰, durante um período de 12 dias. Ao final do processo de aclimatação, as pós-larvas (PL22) foram cultivadas até se obter o peso de 0,3+0,1g e, posteriormente, foram transferidas para 18 tanques de fibra de vidro (800L de volume útil) e estocadas com 150 PL/tanque. A porcentagem de adição de substrato (PAS) foi representada por 100, 150, 200, 250 e 300% da área de fundo dos tanques (1,0 m2), e o controle com “0” de PAS, analisadas em delineamento inteiramente casualizado, com três repetições. Durante a fase de cultivo, foi utilizada, nos 30 primeiros dias, ração com40% de proteína bruta (PB) e nos 15 posteriores, com 35% PB, administrada diariamente às 8, 12 e 17 horas. Ao final de 45 dias de cultivo, a sobrevivência variou de 45 a 84%, entre os tratamentos com 300% e 100% de substrato, respectivamente. O peso final dos camarões, quando relacionado ao comprimento orbital e ao tempo de cultivo, não teve diferença estatística entre as PAS (P>0,05). Na relação entre comprimento com o tempo de cultivo, as PAS de 0, 200 e 250% diferenciaram de 300%. Conclui-se que o uso de substrato artificial favorece as variáveis ganho de peso, fator de conversão alimentar, taxa de crescimento diária e sobrevivência quando se utiliza PAS entre 87,8 e 166,7%.

Submitted by (edna.saturno@ufrpe.br) on 2017-02-08T13:20:12Z No. of bitstreams: 1 Beatriz Regina Brito de OLiveira.pdf: 480535 bytes, checksum: e11bc6a9de00800c4c22f9a20e2b021b (MD5) Made available in DSpace on 2017-02-08T13:20:12Z (GMT). No. of bitstreams: 1 Beatriz Regina Brito de OLiveira.pdf: 480535 bytes, checksum: e11bc6a9de00800c4c22f9a20e2b021b (MD5) Previous issue date: 2008-08-28 The white shrimp Litopenaeus vannamei is the species that stands out most in brazilian shrimp culture. One of the threats to the growth of this activity is the occurrence of diseases that affect the cultured animals, and hence, one of the actions taken to prevent or treat sick animals, the use of antibiotics, such as oxytetracycline (OTC). High Performance Liquid Chromatography (HPLC) with Detection Array of Diodes (DAD) was used to evaluate the OTC’s levels in shrimps cultured in tanks and treated with the drug. After the end of the treatment of shrimps with medicated feed containing OTC at 0,2; 0,4 and 0,5 mg/kg for 14 days, the elimination of antibiotic in muscle and the carapace of the animals until 22 days after medication was evaluated. It was verified through the results that the validated method intended to use and the OTC depletion was greater in carapace of animals (from 10 to 13 days) when compared to the muscle (five days). There was no statistical difference (P 0,05) between treatments used for levels of OTC in muscle and carapace. It is essential to point out the importance of quality control of drugs used in aquaculture as well as the deficient review of national and international standards about the use in the analyses of the shrimp whole, and not just the muscle, to evaluate the accordance of OTC residue in sample of crustaceans. O camarão marinho Litopenaeus vannamei é a espécie que mais se destaca na carcinicultura brasileira. Uma das ameaças ao crescimento desta atividade em todo o mundo é a incidência de enfermidades que afetam os animais cultivados, sendo por isso, uma das possíveis ações adotadas para prevenir ou tratar animais enfermos é o uso de antibióticos, como a oxitetraciclina (OTC). Sendo assim, a partir de uma metodologia validada baseada na técnica de cromatografia líquida de alta eficiência (CLAE) com detecção por arranjo de diodos (DAD), avaliaram-se os níveis de oxitetraciclina em camarões cultivados em tanques e alimentados com ração adicionada do antibiótico objetivando-se avaliar a metodologia. Após o término do tratamento dos camarões com OTC nas concentrações de 0,2; 0,4 e 0,5 mg/Kg de ração durante 14 dias foi avaliada a concentração deste antibiótico no músculo e na carapaça dos animais até 22 dias após suspensão da medicação. Verificou-se que o método validado atende ao uso pretendido e foi observado maior tempo de residência da droga na carapaça dos animais cultivados em experimento (de 10 a 13 dias) quando comparado ao do músculo (cinco dias). Não houve diferença estatística (P 0,05) entre os tratamentos utilizados em relação aos níveis de OTC no músculo e na carapaça. É importante ressaltar a importância de um maior controle da qualidade dos fármacos utilizados na aquicultura, assim como, a falta de revisão de normas nacionais e internacionais quanto à utilização nas análises do camarão inteiro, e não somente do músculo, para avaliação da conformidade de crustáceos quanto ao resíduo de OTC.

Submitted by Mario BC (mario@bc.ufrpe.br) on 2017-11-06T14:36:09Z No. of bitstreams: 1 Ana Cecilia Mamede Garcia.pdf: 1986623 bytes, checksum: a2e063d73475c143e1f43593ed5fc125 (MD5) Made available in DSpace on 2017-11-06T14:36:09Z (GMT). No. of bitstreams: 1 Ana Cecilia Mamede Garcia.pdf: 1986623 bytes, checksum: a2e063d73475c143e1f43593ed5fc125 (MD5) Previous issue date: 2008-08-27 The status of the two distinct the species Rhizoprionodon porosus and Rhizoprionodon terraenovae is not yet ascertained. The identification at the species level of fishery resources is imperative not only for their own ecologic value, but also under the point of view of their management. Multivariate analyses of a set of phenotypic characters can be considered as an appropriate method to elucidate morphologic relations between different species or populations. The morphologic discrimination standards between Rhizoprionodon porosus and Rhizoprionodon terraenovae has been studied through techniques of multivariate analysis with the aim to evaluate the status of the species and to determine which morphometric characters can be used in the discrimination. On basis of 79 individuals collected in 3 localities (Pernamuco e Rio Grande do Norte, Brasil e Flórida, EUA) and 67 paratyps of both species deposited in the Museums MN/UFRJ, MZUSP, MOVI, MNHN and FMNH, Multivariate analyses have been carried out on the basis of 64 morphometric measurements. The results indicate differences between Rhizoprionodon porosus and Rhizoprionodon terraenovae. Distinct groups of both species formed clusters and were analyzed by discriminant analyzes. Units with divergent classification correspond to the region of Central America. Overall, differences suggested that they are two distinct simpatricas species highly miscegenated in the area of Central America. O status atual de Rhizoprionodon porosus e R. terraenovae quanto a constituírem espécies distintas ainda não é bem definido. A identificação das espécies que compõe um recurso pesqueiro é imperativa não somente pela importância ecológica, mas também do ponto de vista da gestão do recurso. A análise multivariada de um conjunto de caracteres fenotípicos é um dos métodos apropriados para esclarecer relações morfológicas de diferentes populações de diferentes espécies. Os padrões de discriminação morfológica entre Rhizoprionodon porosus e Rhizoprionodon terraenovae foram estudados através de técnicas de análise estatística multivariada, com o objetivo de avaliar o status das espécies e determinar se os caracteres morfométricos podem ser usados na discriminação. Com base em uma amostra de 79 indivíduos coletados em 3 localidades (Pernamuco e Rio Grande do Norte, Brasil e Flórida, EUA) e 67 parátipos de ambas as espécies depositados nos Museus MN/UFRJ, MZUSP, MOVI, MNHN e FMNH, analises multivariadas foram realizada com base em 64 medidas morfométricas. Os resultados indicaram diferenças significativas entre exemplares de R. porosus e R. terraenovae. Grupos distintos de ambas espécies foram formados tanto na análise de agrupamento quanto na análise discriminante, exemplares com classificação divergente da análise são em maioria provenientes da América Central. Os resultados indicam que R. terraenovae e R. porosus podem ser consideradas espécies distintas, simpátricas apresentando miscigenação na área da América Central.

Submitted by Mario BC (mario@bc.ufrpe.br) on 2016-08-02T13:31:09Z No. of bitstreams: 1 Cassia Aldrin de Melo.pdf: 1765038 bytes, checksum: 9f36b806c20bd8ef7b5100847812da9b (MD5) Made available in DSpace on 2016-08-02T13:31:09Z (GMT). No. of bitstreams: 1 Cassia Aldrin de Melo.pdf: 1765038 bytes, checksum: 9f36b806c20bd8ef7b5100847812da9b (MD5) Previous issue date: 2008-08-01 Cuiabá river basin, in Mato Grosso State is one of means river receivers of domestic sewage and industries effluents, for them, fishes captured can become contaminated and to be a dangerous to consumer, being rasing of fishes capitivity a viable alternative to provide better quality fish. Specimes of Brycon microleps (piraputanga) captured both from the captivity and from the river on region were studied microbiologically about the mesophiles, total and termtolerants coliforms, Aeromonas and Salmonella, on rain season, and dry season. About specimes were collected liver and encephalo, and to the comparation of values were used the test of correlation of Spearman and the Kolmogorov-Smirnov test. It was verified the sazonality effect (month of collection) over the counting of mesophile, termtolerants coliforms and Aeromonas, at both fish obtained from captivity and river. Higer contamination were observed in finish of dry season and all dry season, then, at most months hots, between August and March. The isolates were A. hydrophila and A. caviae from captivity fishes and A. sobria and A. hydrophila from river fishes, and a relationship was observed between the Aeromonas total number and the coliforms level, in other words, as higher the level of coliforms in sample, higher they were the Aeromonas spp. countings, being the high countings and the low or null, verified in the same months. Salmonella spp. was isolated at one river sample and one of captivity sample. Escherichia coli was isolated in both the fishes, of indicative that some way the water of the two atmospheres was polluted with feces. It was verified that the probability of contamination by mesophiles occurrence in river collected fish is the same as captivity ones (P≥0,05). A bacia do rio Cuiabá no estado de Mato Grosso é uma das principais receptoras de esgotos domésticos e industriais e por isto, os peixes nela capturados podem estar contaminados e representar perigo ao consumidor, sendo a criação de peixes em cativeiro uma alternativa para a obtenção de peixes microbiologicamente melhor. Exemplares de Brycon microleps (piraputanga) oriundos do rio Cuiabá e de cultivo na região foram analisados quanto à bactérias mesófilas, coliformes, Aeromonas e Salmonella, nos meses de chuva e seca. Dos exemplares foram utilizados fígado e encéfalo, e para a comparação dos dados utilizou-se a correlação de Spearman e o teste de Kolmogorov-Smirnov. Verificou-se o efeito da sazonalidade (mês de coleta) sobre a contagem de bactérias mesófilas, coliformes termotolerantes e Aeromonas, tanto em peixes capturados no rio como nos cultivados, sendo as maiores contagens observadas no final do período seco e no início do período chuvoso, ou seja, nos meses mais quentes do ano, entre agosto e março. Foram isoladas A. hydrophila e A. caviae dos peixes de viveiro e A. sobria e A. hydrophila dos peixes do rio e observou-se uma relação entre a contagem de Aeromonas e coliformes, ou seja, quanto maior a contagem de coliformes da amostra maiores foram as contagens de Aeromonas. A Salmonella spp. foi isolada de uma amostra de rio e uma de viveiro. Escherichia coli foi isolada nos peixes de rio e de viveiro, indicando que a água dos ambientes estava contaminada com fezes. Verificou-se que a probabilidade de ocorrência da contaminação por mesófilos em peixes capturados no rio, é a mesma nos obtidos em cultivo (P≥0,05).

Submitted by (edna.saturno@ufrpe.br) on 2016-10-18T11:49:13Z No. of bitstreams: 1 Lilian Maria Nery de Barros Goes.pdf: 490274 bytes, checksum: f5fc8ea054d8a75de4f16f6764893075 (MD5) Made available in DSpace on 2016-10-18T11:49:13Z (GMT). No. of bitstreams: 1 Lilian Maria Nery de Barros Goes.pdf: 490274 bytes, checksum: f5fc8ea054d8a75de4f16f6764893075 (MD5) Previous issue date: 2008-07-02 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES The blood cells normal values knowledge is one of the most valuable clinical labs technique which allows the systemic and morphologic anomaly, when identification is not cleary shown in the organism, identification. At the mammals medicine these values are great known, but on the shrimp culture there still be too much to clear up about haemolynph. Several studies have been realized aiming determines the different kinds of cells and their respective functions. However, the important information like the normal values of the hemocytes total counting (HTC) in different development phases to the Litopenaeus vannamei species and the existence of hemocytes behavior variations on males and females, as well as its relation to anatomo-histopatologic alterations, still being unknown. In face of the exposed, it was aimed to verify the hemocytes behavior on the different cultivated L. vannamei shrimp development phases, the sexual quantitative variation existence, the sodium citrate and Ethylenediaminetetraacetic acid (EDTA) efficacy as anticoagulant, beyond its relation to the detected alterations at the fresh and histopathology exams. 20 samples of haemolynph were collected weekly, being ten (five males and five females) using the sodium citrate anticoagulant and ten using the EDTA anticoagulant. The collects were realized in two aquariums (A and B), on summer and winter times at a commercial shrimp culture located in Goiana/Pernambuco estate. 0,1mL of haemolynph on a proportion of 1:1 (anticoagulant with 10%concentration:haemolynph) were collected from each shrimp to clinical pathology analyses. The hemocyties/mm3counting was realized immediately after the collect and after a five hours interval. The results were statistically analyzed using multiple linear moulds (P

Submitted by (ana.araujo@ufrpe.br) on 2016-07-06T16:01:03Z No. of bitstreams: 1 Iran Rodrigues de Oliveira.pdf: 478669 bytes, checksum: d4813a6f79bb512c0c9744fe30e4adef (MD5) Made available in DSpace on 2016-07-06T16:01:03Z (GMT). No. of bitstreams: 1 Iran Rodrigues de Oliveira.pdf: 478669 bytes, checksum: d4813a6f79bb512c0c9744fe30e4adef (MD5) Previous issue date: 2008-04-29 In this work, Data Envelopment Analysis was used to evaluate efficiency of marine shrimp Litopenaeus vannamei culture winth freshwater systems, considering relation between output variables (production and final mean weight) and input variables (culture production time, post larvae quantity, pond area, ration quantity), offered (pH, water transparency, ration brand and post larva e hatchery gounce). The database used for this study belongs to a commercial farm at Rio Grande do Norte – Brasil, composed of 115 observations collected from August 2008 to October 2005. Application of DEA indicates that 24 cultures were efficient, while 91 were not efficienty (efficiency less then). For each of the inefficient cultures individual efficiency optimization goals were established. It was also found that among the exogenous variables only temperature was related with the production efficiency in terms of final mean weignt shrimp, while dissolved oxygen pH, water transparency, ration brand and post larvae source do not demonstrate significant relevance (p ≥ 0,05). Para avaliar a eficiência do cultivo do camarão marinho Litopenaeus vannamei, em sistema dulciaqüicola, utilizou-se a técnica da Análise Envoltória dos Dados (Data Envelopment Analysis - DEA), considerando as variáveis de saída (produtos) produção e peso médio final quando associadas às de entrada (insumos) tempo de cultivo, quantidade de pós-larva, área do viveiro, quantidade de ração ofertada e as variáveis exógenas, marca de ração e laboratório de origem das póslarvas. Utilizou-se um banco de dados de uma fazenda comercial do Rio Grande do Norte – Brasil, composto por 115 observações, o qual foi gerado durante o período de Agosto de 2002 a Outubro de 2005, sendo utilizado o sistema bifásico (fase berçário e fase engorda). Com a técnica DEA evidenciaram-se 24 cultivos eficientes e 91 ineficientes (eficiência

Submitted by (edna.saturno@ufrpe.br) on 2017-02-22T15:35:53Z No. of bitstreams: 1 Ugo Lima Silva.pdf: 369837 bytes, checksum: fb82abdaa84e171bd675e0309f403a29 (MD5) Made available in DSpace on 2017-02-22T15:35:53Z (GMT). No. of bitstreams: 1 Ugo Lima Silva.pdf: 369837 bytes, checksum: fb82abdaa84e171bd675e0309f403a29 (MD5) Previous issue date: 2008-04-01 This work investigated the effect of molasses addition in different C:N ratios on the water quality and growth performance of the Litopenaeus vannamei shrimp during the nursery phase without water exchange. A randomized experimental design was applied with three treatments and four replicates. Two treatments with daily molasses addition of 25:1 (25M) and 15:1 (15M) C:N ratio and one control with no carbon source addition (0M). Twelve (800 L) fiber glass tanks were stocked with 6.25 post-larvae/L (mean weight < 0.01 g) corresponding to 5000 post larvae/tank. Shrimps were fed with a 40% crude protein commercial diet offered at 08:00, 11:00, 14:00 and 17:00. The feedding rate varied from 50 to 15% of the shrimp biomass from the beginning to the end of the culture. The water temperature, dissolved oxygen and pH were measured daily whereas transparency and salinity were measured weekly. The 25:1 and 15:1 C:N ratios showed lower (p0.05). Thisstudy indicates that the molasses can be used as carbon source in order to increase C:N ratio, improving the water quality and L. vannamei growth performance with no water exchange. O presente estudo investigou o efeito da adição de melaço em diferentes relações C:N sobre a qualidade da água e desempenho de crescimento do camarão Litopenaeus vannamei, na fase berçário, em tanques de cultivo sem renovação de água. Adotou-se um delineamento inteiramente casualizado com três tratamentos e quatro réplicas, sendo dois com aplicação diária de melaço nas relações C:N 25:1 (25M) e 15:1 (15M), e um controle (0M), sem aplicação desta fonte de carbono. Foram utilizados 12 tanques em fibra de vidro (800 L de volume útil), estocados com 6,25 pós-larvas/L (peso médio < 0,01 g), totalizando 5000 póslarvas/ tanque. A alimentação constou de ração comercial contendo 40% de proteína bruta ofertada diariamente às 8, 11, 14 e 17h, numa taxa de alimentação variando de 50 a 15% da biomassa, do início ao final do cultivo. Temperatura da água, oxigênio dissolvido e pH foram aferidos diariamente, enquanto que transparência e salinidade foram aferidas semanalmente. As relações C:N 25:1 e 15:1 apresentaram níveis de pH 7,9, que foram inferiores (p0,05) entre os mesmos. Desta forma, o melaço pode ser utilizado como fonte de carbono para incrementar a relação C:N, melhorando a qualidade da água e o desempenho de L. vannamei sem renovação de água, na fase berçário.

Submitted by (ana.araujo@ufrpe.br) on 2016-08-08T15:17:09Z No. of bitstreams: 1 Moacy Vasconcelos Cabral.pdf: 608103 bytes, checksum: 99d5c81824432310afaa742f16c76e41 (MD5) Made available in DSpace on 2016-08-08T15:17:09Z (GMT). No. of bitstreams: 1 Moacy Vasconcelos Cabral.pdf: 608103 bytes, checksum: 99d5c81824432310afaa742f16c76e41 (MD5) Previous issue date: 2008-02-28 There are several factors that are responsible for the most deaths caused by cardiovascular diseases, which are growing in almost all countries of the world. Acute myocardial infarction (AMI) is the one that occurs with the greatest extent, originating from the partial or total occlusion of one or more coronary arteries – the atherosclerosis. Throughout the years, various techniques were implemented to diagnose as well as to treat coronary diseases. In the search for a better quality of life and a less invasive treatment than the traditional surgeries for myocardial revascularization, angioplasty is applicable. Unfortunately, around 30% of the patients treated with angioplasty, in a period of just six months, come back to treatment due to restenosis – a reoccurrence of the narrowing or blockage in the blood vessel. Nowadays, Intravascular Brachytherapy – a technique with intravenous radiation – is the method of choice as it handles this phase in a satisfactory way, for it is observed the receding of the proliferative metabolism of restenosis. Thus, with the aim for radiological protection of both patients and professionals involved in the therapy, the distribution of doses for the monoenergetic electrons and photons were calculated for blood vessels of 4.5, 3.0 and 1.5 mm of diameter in several sources, using the MCNP4B code. In this context, the goal of this work was to classify theradioactive sources used in Intravascular Brachytherapy by employing Cluster Analyses with related dendograms and Cophenetic Correlation Coefficients. The results showed that the hierarchical techniques were effective in the classification of the electron-emitting radioactive sources (Cophenetic Correlation Coefficients varied from 0.81 to 0.99), whereas for the photons these techniques were not satisfactory (Cophenetic Correlation Coefficients varied from 0.64 to 0.78). Existem vários fatores que são responsáveis pela grande maioria das mortes causadas por doenças cardiovasculares as quais são crescente em quase todos os países do mundo, sendo o infarto agudo do miocárdio (IAM) a que ocorre com maior destaque, oriundo de oclusão parcial ou total de uma ou mais artérias coronárias (a aterosclerose). Atualmente, vêm sendo aplicadas diversas técnicas tanto para diagnosticar quanto para tratar a doença coronariana. Buscando uma melhor qualidade de vida e um tratamento menos invasivo do que as tradicionais cirurgias de revascularização do miocárdio, emprega-se a angioplastia coronária. Lamentavelmente, em torno de 30% dos pacientes tratados por angioplastia, em um período de apenas seis meses, voltam ao médico devido à reestenose (reestreitamento da artéria). Recentemente, a braquiterapia intravascular (terapia com radiação intravaso) vem sendo bastante útil no retardo do metabolismo proliferativo da reestenose. Dessa forma, visando à proteção radiológica dos pacientes e profissionais envolvidos nesta terapia, cálculos de dose foram realizados em vasos sanguíneos de 4,5, 3,0 e 1,5 mm de diâmetro para diversas fontes de radiação,usando o código MCNP4B. Neste contexto, o objetivo do presente trabalho foi o de classificar fontes radioativas usadas na Braquiterapia Intravascular utilizando a análise de agrupamento, por meio de dendograma e Correlação Cofenética associados. Os resultados mostraram que as técnicas hierárquicas foram eficientes na classificação das fontes radioativas emissoras de elétrons (as Correlações Cofenéticas variaram de 0,81 a 0,99), enquanto que para os fótons essas técnicas não foram satisfatórias (as Correlações Cofenéticas variaram de 0,64 a 0,78).

Submitted by (edna.saturno@ufrpe.br) on 2016-08-17T16:26:34Z No. of bitstreams: 1 Cassia Aldrin de Mello.pdf: 4250938 bytes, checksum: 88a7fbf54f3c43c0895924fbf26b6c5d (MD5) Made available in DSpace on 2016-08-17T16:26:34Z (GMT). No. of bitstreams: 1 Cassia Aldrin de Mello.pdf: 4250938 bytes, checksum: 88a7fbf54f3c43c0895924fbf26b6c5d (MD5) Previous issue date: 2008-02-14 Cuiabá river basin, in Mato Grosso State is one of means river receivers of domestic sewage and industries effluents, for them, fishes captured can become contaminated and to be a dangerous to consumer, being rasing of fishes capitivity a viable alternative to provide better quality fish. Specimes of Brycon microleps (piraputanga) captured both from the captivity and from the river on region were studied microbiologically about the mesophiles, total and termtolerants coliforms, Aeromonas and Salmonella, on rain season, and dry season. About specimes were collected liver and encephalo, and to the comparation of values were used the test of correlation of Spearman and the Kolmogorov-Smirnov test. It was verified the sazonality effect (month of collection) over the counting of mesophile, termtolerants coliforms and Aeromonas, at both fish obtained from captivity and river. Higer contamination were observed in finish of dry season and all dry season, then, at most months hots, between August and March. The isolates were A. hydrophila and A. caviae from captivity fishes and A. sobria and A. hydrophila from river fishes, and a relationship was observed between the Aeromonas total number and the coliforms level, in other words, as higher the level of coliforms in sample, higher they were the Aeromonas spp. countings, being the high countings and the low or null, verified in the same months. Salmonella spp. was isolated at one river sample and one of captivity sample. Escherichia coli was isolated in both the fishes, of indicative that some way the water of the two atmospheres was polluted with feces. It was verified that the probability of contamination by mesophiles occurrence in river collected fish is the same as captivity ones (P 0,05). A bacia do rio Cuiabá no estado de Mato Grosso é uma das principais receptoras de esgotos domésticos e industriais e por isto, os peixes nela capturados podem estar contaminados e representar perigo ao consumidor, sendo a criação de peixes em cativeiro uma alternativa para a obtenção de peixes microbiologicamente melhor. Exemplares de Brycon microleps (piraputanga) oriundos do rio Cuiabá e de cultivo na região foram analisados quanto à bactérias mesófilas, coliformes, Aeromonas e Salmonella, nos meses de chuva e seca. Dos exemplares foram utilizados fígado e encéfalo, e para a comparação dos dados utilizou-se a correlação de Spearman e o teste de Kolmogorov-Smirnov. Verificou-se o efeito da sazonalidade (mês de coleta) sobre a contagem de bactérias mesófilas, coliformes termotolerantes e Aeromonas, tanto em peixes capturados no rio como nos cultivados, sendo as maiores contagens observadas no final do período seco e no início do período chuvoso, ou seja, nos meses mais quentes do ano, entre agosto e março. Foram isoladas A. hydrophila e A. caviae dos peixes de viveiro e A. sobria e A. hydrophila dos peixes do rio e observou-se uma relação entre a contagem de Aeromonas e coliformes, ou seja, quanto maior a contagem de coliformes da amostra maiores foram as contagens de Aeromonas. A Salmonella spp. foi isolada de uma amostra de rio e uma de viveiro. Escherichia coli foi isolada nos peixes de rio e de viveiro, indicando que a água dos ambientes estava contaminada com fezes. Verificou-se que a probabilidade de ocorrência da contaminação por mesófilos em peixes capturados no rio, é a mesma nos obtidos em cultivo (P 0,05).

Submitted by (edna.saturno@ufrpe.br) on 2017-02-14T15:59:47Z No. of bitstreams: 1 Joao Paulo Viana de Lima.pdf: 751024 bytes, checksum: 12aea5280a52c50190be7cbe5d4985dc (MD5) Made available in DSpace on 2017-02-14T15:59:47Z (GMT). No. of bitstreams: 1 Joao Paulo Viana de Lima.pdf: 751024 bytes, checksum: 12aea5280a52c50190be7cbe5d4985dc (MD5) Previous issue date: 2007-04-27 The accumulation of toxic nitrogenous compounds in the water is a common problem to aquaculture systems. Recent works have showed good results in terms of production and nitrogen retention efficiency, through the addition of organic carbon source (sugar, molasses, etc). This work investigated the effect of molasses addition in different C:N ratios on the water quality, microbial activity and production, in semi-intensive experimental culture tanks of Litopenaeus vannamei with no water exchange. Four treatments and three replicates were adopted, which three with daily molasses addition in 5, 10 and 15:1 C:N ratio and one control with no carbon source addition. Twelve 500 L fiber glass tanks were stocked with 25 shrimps.m-2 (1.90±0.37 g). Shrimps were fed with a 35% crude protein commercial diet offered in feeding trays at 08:00 and 16:00. Phytoplankton, bacteria and water samples were collected fortnightly during the culture. The 15:1 and 10:1 C:N ratios showed lower (P

Submitted by (edna.saturno@ufrpe.br) on 2016-08-11T16:51:11Z No. of bitstreams: 1 Carlos Andre Bezerra Alves.pdf: 107646 bytes, checksum: c97f6b2e6468643c3953a5613da56559 (MD5) Made available in DSpace on 2016-08-11T16:51:11Z (GMT). No. of bitstreams: 1 Carlos Andre Bezerra Alves.pdf: 107646 bytes, checksum: c97f6b2e6468643c3953a5613da56559 (MD5) Previous issue date: 2007-03-22 Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPq Diseases means any adverse alteration in the health or acting in the productivity of the animals. In shrimps, the diseases can be unchained when it occur an unbalance potentially among the environmental conditions of nurseries, the health condition of the cultivated shrimps and the pathogenic agents, associated with the employed handling. This way, it was aimed at to evaluate the sanitary conditions and to determine which the relevant factors in the attack of the vibrioses in shrimp cultivated Litopenaeus vannamei. In the period of December of 2005 to November of 2006, were collected and analyzed shrimp samples, water and nursery soil, as well as technical information on the handling adopted in a commercial shrimp culture located in the north of Pernambuco/ Brazil. The handling parameters were correlated through mathematical models (P

Submitted by (edna.saturno@ufrpe.br) on 2017-02-22T12:37:59Z No. of bitstreams: 1 Suely Santos Bezerra.pdf: 418523 bytes, checksum: 6cbf43e2cf1c398824dd661f7ea8c038 (MD5) Made available in DSpace on 2017-02-22T12:37:59Z (GMT). No. of bitstreams: 1 Suely Santos Bezerra.pdf: 418523 bytes, checksum: 6cbf43e2cf1c398824dd661f7ea8c038 (MD5) Previous issue date: 2007-02-23 It can be observed nowadays that the increase in the commercial shrimp produc-tion occurs concomitantly with an enhancement of the incidence of diseases. These last ones can cause innumerable damages to the shrimp farmers in the whole world. Amongst these diseases, it can be distinguished the Infectious Hypodermal and Hema-topoietic Necrosis (IHHN), with viral etiology, that can be characterized by not causing great mortality, but body deformities. In addition to this, it frequently leads shrimp to heterogenic growth. For viruses diagnosis histology is an efficient tool, allowing the verification of the health of these crustaceans. This study aimed the observation of the occurrence of IHHN characteristic histopatologic injuries in Litopenaeus vannamei, and its relation with parameters of culture of marine shrimp farms in the state of Per-nambuco. After multiple regression statistic analysis it could be concluded that the tem-perature of the water of the pound and its oxygen availability are narrowly related with the occurrence of those histopatologic injuries. Ao mesmo tempo em que crescem os cultivos comerciais de camarão, observa-se o aumento da incidência de enfermidades que podem causar inúmeros prejuízos aos carcinicultores em todo o mundo. Dentre essas enfermidades, destaca-se a Infecção Hi-podermal e Necrose Hematopoiética (IHHN), de etiologia viral que, embora não cause grandes taxas de mortalidade, produz retardo no desenvolvimento e deformidades nos camarões. Para o diagnóstico dessa virose a histologia tem sido uma ferramenta bastante eficiente e que permite maior monitoramento da sanidade desses crustáceos. Desta for-ma, objetivou-se estudar a IHHN em Litopenaeus vannamei relacionando lesões histo-patológicas características e sua relação com variáveis de cultivo. Com base em regres-sões múltiplas conclui-se que existe uma alta relação entre as variáveis de cultivo tem-peratura e oxigênio e a ocorrência destas lesões.

Submitted by (edna.saturno@ufrpe.br) on 2017-02-22T16:21:30Z No. of bitstreams: 1 Veronica Arns da Silva.pdf: 500226 bytes, checksum: 2a21789fca9964130708e123521a1f6c (MD5) Made available in DSpace on 2017-02-22T16:21:30Z (GMT). No. of bitstreams: 1 Veronica Arns da Silva.pdf: 500226 bytes, checksum: 2a21789fca9964130708e123521a1f6c (MD5) Previous issue date: 2007-02-22 Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPq The Infectious Myonecrosis Viral (IMNV), restricted action to the Brazilian northeast up to 2006, is disease of bigger negative impact in the shrimp cultured of the region. Having the objective to verify occurrence and evolution of the IMNV in Litopenaeus vannamei cultured on the coast of Pernambuco, Brazil, histopathological examination in 60 samples of shrimps from four farms in two cycles of aquaculture was proceeded. The histopatological findings had been correlated the data of the wet mount and the inquiry. Suggestive injuries of IMNV (coagulation necrosis, muscular hemocitic infiltration, spheroid of the ectopic lynphoid organ) were found in samples of the four studied farms with bigger occurrences in the first cycle. However, in the rainy period it had reduction of the occurrence, being associated the handling change. The raise time and the density were variables which influenced significantly (P

Submitted by (edna.saturno@ufrpe.br) on 2017-02-21T16:11:44Z No. of bitstreams: 1 Roseli Pimentel Pinheiro e Silva.pdf: 245935 bytes, checksum: 769eb187840e50bd513ec06abe7683e0 (MD5) Made available in DSpace on 2017-02-21T16:11:44Z (GMT). No. of bitstreams: 1 Roseli Pimentel Pinheiro e Silva.pdf: 245935 bytes, checksum: 769eb187840e50bd513ec06abe7683e0 (MD5) Previous issue date: 2007-02-22 Considering the illnesses are barriers for the development of the carcinicultura, the vibriose has the possibility to occur for having as etiologic agent bacteria autochtonous of marine environment, it was objectified identification of the interferentes factors in the frequency of this disease. Four situated farms in the south coast of Pernambuco had been selected of which samples of water and shrimp during all had been collected monthly the phases of culture in two different climatic cycles (wet and rainy). Esteem the load of Vibrio spp., samples of water, post larvae had been analyzed, hemolymph and hepatopancreas and the countings had been correlated, through the use of mathematical models (P

Submitted by (edna.saturno@ufrpe.br) on 2017-02-07T12:21:56Z No. of bitstreams: 1 Ana Helena Gomes da Silva.pdf: 731808 bytes, checksum: e9fd8928b9e2cb379f53ffb4a436b8a7 (MD5) Made available in DSpace on 2017-02-07T12:21:56Z (GMT). No. of bitstreams: 1 Ana Helena Gomes da Silva.pdf: 731808 bytes, checksum: e9fd8928b9e2cb379f53ffb4a436b8a7 (MD5) Previous issue date: 2006-04-28 Commercial culture of marine shrimp Litopenaeus vannamei were done in Rio Grande do Norte state from 08/08/2002 to 10/15/2005. Were used statistical models to correlate the production variables (production, productivity, survival rate, feed convection ratio, final mean weight and offered feed quantity) with the management and water quality ones (number of ponds, number of cycles, recirculation system, season of the year, days of culture, stocking density, postlarvae source, ration brand, oxygen, temperature, pH and transparency). It was used the stepwise method associated to Box and Cox transformer to select the variables more significatives in each model. It was verified that the management variables with more correlation were ration brands, hatchery of postlarvae source and days of culture. Dados de obtidos de cultivos comerciais do camarão marinho Litopenaeus vannamei realizados no Estado de Rio Grande do Norte, no período de 08/08/2002 a 15/10/2005. Foram utilizados modelos estatísticos para correlacionar as variáveis de produção (produção, produtividade, taxa de sobrevivência, fator de conversão alimentar, peso médio final e quantidade de ração ofertada), com as de manejo (número de viveiros, número de ciclos, sistema de recirculação, estação do ano, tempo de cultivo, densidade de estocagem, laboratório de origem das pós-larvas, marca da ração) e qualidade de água (oxigênio, temperatura, pH e transparência). Para selecionar as variáveis mais significativas em cada modelo, utilizou-se o método de Stepwise associado ao transformador de Box e Cox. Verificou-se que as variáveis de manejo mais correlacionáveis foram marcas das rações, os laboratórios de origem das pós-larvas e tempo de cultivo.

Submitted by (edna.saturno@ufrpe.br) on 2017-02-09T16:26:01Z No. of bitstreams: 1 Edson Pereira dos Santos.pdf: 1722361 bytes, checksum: a0364ce831329e35264c970b58fe0613 (MD5) Made available in DSpace on 2017-02-09T16:26:01Z (GMT). No. of bitstreams: 1 Edson Pereira dos Santos.pdf: 1722361 bytes, checksum: a0364ce831329e35264c970b58fe0613 (MD5) Previous issue date: 2006-04-28 The freshwater prawn Macrobrachium carcinus production had been commercially explored in several countries. In northeast, the prawn fishery has a great importance in Low São Francisco Basin. The large scale postlarvae production continues being the main problem for commercial culture and natural stocks maintenance. This work aimed to test different diets in Macrobrachium carcinus larval culture in order to improve the performance of prawn post-larvae production, with the utilization of fish flesh (Ff) and a formulated diet (Fd), or in association with adult artemia biomass (FfB and FdB, respectively), resulting in four treatments and six replicates. Were used twenty-four 20 L circular recipients provided of water recirculating and aeration systems, where were stocked 25 larvae (zoea V-VI) per liter. The diets were offered four times a day (07:00, 10:00, 13:00 and 16:00 hrs) during 49 days. Water temperature was 27.5±1.2ºC in the morning and 28.4±1.3ºC in the afternoon; the total ammonia nitrogen and nitrite were 0.2±0.2 and 0.5±0.8 mg/L, respectively, while pH and salinity maintained around 8.2 and 24, respectively. The average larval survival was 3.47±1.56, 7.40±2.99, 14.83±2.64 and 7.57±2.31, respectively for Ff, FfB, Fd and FdB treatments. Ff treatment obtained the lowest survival due to larvae reject by the fish flesh. Fdand FdB treatments showed significant difference (P≤0.05), with better survival obtained by FdB diet. The highest survival was obtained by the Fd diet (14.83%), that showed itself as the more appropriated alternative to use in M. carcinus hatchery, but the adult artemia biomass showed that could provide better survival rates when associated with other feeds. A produção do camarão pitu Macrobrachium carcinus tem sido explorada comercialmente em diversos países. No Nordeste, a pesca do pitu é de grande importância no Baixo São Francisco. A produção em larga escala de pós-larvas do pitu continua sendo o principal empecilho para o cultivo comercial e recuperação dos estoques naturais. Desta forma, objetivou-se com o presente trabalho testar diferentes dietas na larvicultura de M. carcinus, visando melhorar o desempenho da produção de pós-larvas, com a utilização de músculo de peixe (Dp) e uma dieta formulada (Df), ou em associação à biomassa de Artemia sp (DpB e DfB, respectivamente), perfazendo quatro tratamentos em seis réplicas. Utilizaram-se 24 recipientes circulares de 20 L com sistemas de recirculação de água e aeração, nos quais estocaram-se 25 larvas (zoea V-VI) por litro. As dietas foram ofertadas em quatro horários (07:00, 10:00, 13:00 e 16:00h) durante 49 dias. Durante o cultivo registrou-se valores médios de 27,5 ± 1,2 ºC (manhã) e 28,4 ± 1,3 ºC (tarde) para temperatura, de 0,2 ± 0,2 mg/L para amônia total e de 0,5 ± 0,8 mg/L para nitrito, enquanto que o pH e a salinidade mantiveram-se em 8,2 e 24‰, respectivamente. Os percentuais de sobrevivência média das larvas foram 3,47 ± 1,56, 7,40 ± 2,99, 14,83 ± 2,64 e 7,57 ± 2,31%, respectivamente, para os tratamentos Dp,DpB, Df e DfB. No tratamento Dp obteve-se a menor (P≤0,05) sobrevivência, pelo fato das larvas rejeitarem o músculo de peixe triturado. As dietas Dp e DpB apresentaram diferença significativa (P≤0,05), com maior sobrevivência para a dieta DpB. A maior sobrevivência foi obtida pela dieta Df (14,83%), que se apresentou como a alternativa mais apropriada para a utilização na larvicultura de M. carcinus, entretanto a biomassa de artêmia adulta mostrou que poderá resultar em uma melhoria na taxa de sobrevivência quando associada com outros alimentos.

Submitted by (edna.saturno@ufrpe.br) on 2017-02-07T15:03:38Z No. of bitstreams: 1 Anderson Goncalves Croccia.pdf: 343718 bytes, checksum: ab4281348333734382726c65db799fc0 (MD5) Made available in DSpace on 2017-02-07T15:03:38Z (GMT). No. of bitstreams: 1 Anderson Goncalves Croccia.pdf: 343718 bytes, checksum: ab4281348333734382726c65db799fc0 (MD5) Previous issue date: 2006-04-26 Abrupt changes of pH was conducted to estimate yhe influence on survive of the post-larvae of the srhimp Litopenaeus vannamei, in the 0‰, 10‰, 20‰ and 25‰ salinity. The post-larvaewas estocked of 50 post-larvae/L in a 18 plastic containers with the capacity of 2,5 liters. The pH water was 8,0 in the larviculture. Increase or decrease the pH water in 0,5, 1,0, 1,5, and 2,0 were used the calcium or acid acetic, respectivally. After 48 hours the post-larvae were counted one by one, to obtain the survaive rate. According to a mathematic model applied, it was shown the salinity wasn’t influencied significative (≥0,05) in survive of the post-larvae. Changes of pH to valoes more alcalinity were more impacty in the survive and to 8,0 to 10,0 made 100% of the mortality on the post-larvae. Mudanças bruscas do pH da água foram realizadas, para avaliar sua influência na sobrevivência de pós-larvas do camarão Litopenaeus vannamei, cultivadas em diferentes salinidades 0‰;10‰; 20‰ e 25‰.As pós-larvas foram estocadas a densidade de 50 pós-larvas/L em reservatórios plásticos com capacidade de 2,5 L. O pH inicial da água foi de 8,0, equivalente ao da larvicultura. Para aumentar ou diminuir o pH da água em 0,5, 1,0, 1,5 e 2,0, foram utilizados cal hidratada e ácido acético, respectivamente. Após 48 horas das pós-larvas terem sido submetidas as mudanças de pH da água, estas foram contadas individualmente, para estimar a taxa de sobrevivência. De acordo com o modelo matemático utilizado para correlacionar essas variáveis, verificou-se que a salinidade não influenciou significativamente (P ≥ 0,05) na sobrevivência das pós-larvas. Mudanças de pH para valores mais alcalinos foram mais impactantes na sobrevivência e quando foi de 8,0 para 10, gerou 100% de mortalidade das pós-larvas.

Submitted by (ana.araujo@ufrpe.br) on 2016-05-19T13:15:06Z No. of bitstreams: 1 Ady Marinho Bezerra.pdf: 631885 bytes, checksum: cbc9f196ed10a1296f54624a959cc596 (MD5) Made available in DSpace on 2016-05-19T13:15:06Z (GMT). No. of bitstreams: 1 Ady Marinho Bezerra.pdf: 631885 bytes, checksum: cbc9f196ed10a1296f54624a959cc596 (MD5) Previous issue date: 2006-02-24 It was objectified to correlate of the variable-response (production,productivity, final weight and tax of survival), with the water physic (temperature in the deep one, temperature in the surface and water level) and the chemical (salinity, oxygen in the deep one, oxygen in the surface, pH and transparency of the water) and the involved variable and the adopted handling (amount of ration,period of winter, period of summer, days of culture, initial density, supplying laboratory of after-larva, area of the fishery, number of cycles, initial population,aeration and days of preparation) of the culture of the marine shrimp Litopenaeus vannamei. The data base of the shrimp farm was composed for 68 comments, correspondents to the period of 2003 the 2005. Esteem the parameters of themodels it was used technique of the square minimums. The election of variable was carried through using the process of Stepwise Backward, associated the Box and Cox, to minimize the sum of square errors. The adequacy of the esteem equations and the hypothesis of linearity, normality and homogeneous variance for the errors had been analyzed on the basis of the analysis of variance and in the analysis of residue. According to the results, it was possible to conclude that the water physic and chemical influenced (p < 0.05) the variableness of the variableresponse Objetivou-se correlacionar as variáveis de cultivo (produção, produtividade,peso final e taxa de sobrevivência) com as variáveis físicas (temperatura no fundo do viveiro, temperatura na superfície do viveiro e nível da água do viveiro) e químicas da qualidade da água (salinidade da água, oxigênio no fundo do viveiro,oxigênio na superfície do viveiro, pH da água e transparência da água) e as variáveis envolvidas no manejo adotado (quantidade de ração, período de inverno, período de verão, dias de cultivo, densidade inicial, laboratório fornecedor de pós-larva, área do viveiro, número de ciclos, população inicial, aeração e dias de preparação) do camarão marinho Litopenaeus vannamei, e selecionar as variáveis que mais influenciaram em cada uma das variáveis respostas. O banco de dados da fazenda de camarão foi composto por 68 observações,correspondentes ao período de 2003 a 2005. Para estimar os parâmetros dos modelos se utilizou a técnica dos mínimos quadrados. A seleção de variáveis foi realizada utilizando-se o processo de Stepwise Backward, associado a Box e Cox, para minimizar a soma dos quadrados dos erros. A adequação das equações estimadas e os pressupostos de linearidade, normalidade e homocedasticidade para os erros foram analisados com base na análise de variância e na análise de resíduo. De acordo com os resultados, pôde-se concluir que as variáveis físicas e químicas da água tiveram grande influência (p < 0,05) na variabilidade das variáveis respostas