1. Genomische Populations- und Verwandtschaftsanalyse von österreichischen Apis mellifera mellifera und Apis mellifera carnica Zuchtpopulationen in Hinblick auf Hybridisierung und Zusammensetzung des anzestralen Genpools.
- Author
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DRUML, THOMAS, HEINZE, BERTHOLD, and BOIGENZAHN, CHRISTIAN
- Subjects
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GENETIC variation , *HONEYBEES , *SINGLE nucleotide polymorphisms , *KINSHIP , *SUBSPECIES , *INBREEDING - Abstract
The “dark honeybee” Apis mellifera mellifera, originally the dominating subspecies of the Western honeybee in Central and Northern Europe, is currently threatened by extinction in Austria and its conservation breeding program is carried out by two breeding associations in Salzburg (AMZ – Austrian Mellifera Breeders) and Tyrol (breeding association Kauns-Kaunerberg). In this study, genetic diversity (genomic inbreeding FROH, number of homozygosity segments NROH), the relationship structures and foreign gene proportions in samples from the two Mellifera breeding associations were examined in comparison to A.m.carnica samples using the high-density 70k SNP Illumina® Honey Bee Genotyping Array. The mean Carnica gene-proportion (C-line gene proportion) in the two Mellifera samples was originally 32.4 to 34.4%. By excluding hybridized queens, this gene share was reduced to an average of 12.8–17.7%. In terms of genomic inbreeding, the Austrian Mellifera samples exhibited a mean FROH of 4.20% with maximum values of up to 17.2%. The results of this study clearly illustrated the problems conservation breeding programs in small populations are exposed to. We further demonstrated that the simultaneous consideration of multiple genetic descriptors such as FROH, NROH, ADMIXTURE and the kinship network NETVIEW is effective and practically usable in monitoring breeding programs, which can further be communicated in a way that is understandable to the practical breeders. [ABSTRACT FROM AUTHOR]
- Published
- 2024