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Your search keyword '"Leneuve P"' showing total 2 results
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2 results on '"Leneuve P"'

1. [Effect of interleukins and somatomedins on the production of neurotensin by cell line SH-SY5Y derived from human neuroblastoma]

Author

Loret C, Leneuve P, Bozzola M, Am, Lhiaubet, Rm, Schimpff, and WILLIAM ROSTENE

Subjects
Neuroblastoma, Somatomedins, Interleukins, Tumor Cells, Cultured, Humans, Cell Division, Neurotensin
Abstract

The goal of this study was to investigate the regulation by insulin-like growth factors 1 and 2, and interleukins on the production of neurotensin in the SH-SY5Y cell line derived from a human neuroblastoma. Cultures were performed in RPMI1640 culture medium with heated foetal calf serum 12%. After 24 hrs. of fasting without serum, interleukins-1 alpha, IL-2, IL-4 and insulin-like growth factors 1 and 2 were added. Results showed: 1) A mitogenic effect of ILs (p0.001) and of IGFs (p0.001). 2) The presence of neurotensin in HCl0.1N cellular extracts (0.06 fmol/micrograms protein). 3) The increase of cellular neurotensin content in the presence of IL-4 (560%), IL-2 (480%), IGF-1 (610%) and IGF-2 (200%). Our results indicate that the human neuroblastoma cell line SH-SY5Y produces neurotensin and that ILs and IGFs act in vitro to modulate this production.

Published
1992

2. [Expression regulation of insulin-like growth factor binding proteins (IGFBP). Tissue-specific expression of IGFBP-1].

Author

Groyer A, Babajko S, Hardouin S, Leneuve P, and Binoux M

Subjects
Animals, Humans, Insulin-Like Growth Factor Binding Proteins, Liver Neoplasms, Experimental genetics, Organ Specificity genetics, Promoter Regions, Genetic genetics, Rats, Transcription, Genetic genetics, Transcriptional Activation genetics, Carrier Proteins genetics, Gene Expression Regulation, Insulin-Like Growth Factor I metabolism, Liver
Abstract

We have investigated the liver-specific expression of IGFBP-1 gene, an Insulin-like Growth Factor binding protein. Northern blot as well as transient transfection experiments, both carried out in differentiated (H4II, C2Rev7) and dedifferentiated (H5, C2) hépatoma cell lines, yielded clear cut data allowing the involvement of HNF1, a liver-specific trans acting factor, in basal IGFBP-1 liver-specific expression.

Published
1991

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