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The growth and development of Cypripedium macranthos Sw. are affected by environmental factors, such as temperature, humidity, soil type, and its crown density. In this study, its morphological attributes, physiological traits, and interactions with environmental factors were analysed. The results indicate that the growth of C. macranthos was limited by elevated crown density, soil alkaline-hydrolysed nitrogen, and available soil potassium concentration. Moreover, the physiological attributes of C. macranthos were variably suppressed by high crown density and elevated soil alkaline-hydrolysed nitrogen concentration, whereas enhanced available soil potassium concentration promoted these physiological characteristics. At lower crown density, C. macranthos had greater photosynthetic capacity and higher δ13C, C, and N, which were more favourable for organic matter accumulation and plant growth and development. Conversely, at higher crown densities, plants relied more heavily on mycorrhizal fungi for nutrient acquisition. In conclusion, crown density, soil alkali-hydrolysed nitrogen, and available soil potassium concentration were the main environmental factors influencing the morphological and functional form of C. macranthos. Optimal growth conditions were identified at a crown density of 0.5–0.7, soil alkali-hydrolysed nitrogen concentration of 155.06–246.98 mg/kg, and available potassium concentration of 432.53–502.87 mg/kg. The results of this study provide a theoretical understanding for developing conservation strategies for C. macranthos to ensure the stability of the wild population and the health of its habitats. [ABSTRACT FROM AUTHOR]

This research studies the morphology and morphometry traits of Aseel chickens raised in various regions of Türkiye. We used Turkish Aseel genotypes to assess the live weight and physical features of 60 female and 58 male subjects of varying ages. We measured brood length, breadth, head and beak width, neck and body length, chest width, depth, and circumference, thigh diameter, length, and depth, and leg length. Males' average live weight, chest circumference, thigh length, and comb length, which affect game efficacy, are 3.23 kg, 37.88 cm, 20.69 cm, and 40.20 mm, respectively. Although there was a statistically significant difference between the gender groups for all evaluated characteristics, there was only a statistically significant difference between the age groups for head width and chest circumference, shank depth, diameter, and length, and live weight (P<0.05). The data showed differences among different areas, particularly about the measurements of the comb, prompting researchers to propose a hypothesis suggesting a potential correlation between these changes and regional temperature disparities. We evaluated Turkish Aseels for feather, comb, eye color, markings, and comb type. Medium-weight breeds with hair, earlobes, beards, and spurs Weight and characteristics determine the Turkish Aseel breed. Comb structures were shorter, and their live weight was lower than that of other game roosters or Aseel kinds. Based on their morphology, domestic Aseel males may have a distinct genotype and subtype. Evaluating morphometric characteristics is an important aspect of the research. However, conducting additional comparative research is crucial. Genotyping studies with larger numbers are necessary for greater accuracy. [ABSTRACT FROM AUTHOR]

This study explores the application of sustainable adaptive reuse strategies in preserving historical and cultural buildings, with the goal of establishing a scientifically robust framework of indicators for systematic evaluation and practical application. Focusing on the diaojiaolou (stilted buildings) along the Tuojiang River in the ancient town of Fenghuang, the research integrates findings from an extensive literature review with a theoretical framework to propose a multidimensional indicator system encompassing preservation, rehabilitation, urban renewal, adaptive reuse, urban revitalization, and restoration. Through detailed field investigations and comprehensive case studies, the study examines the cultural significance of the stilted buildings, including their spatial organization, structural design, material composition, and decorative features. It also critically assesses the effectiveness of sustainable adaptive reuse strategies in promoting regional development. The findings reveal that these strategies not only preserve the cultural and historical integrity of the stilted buildings but also enhance their functionality to meet modern needs, achieving a balance among heritage conservation, environmental sustainability, and socio-economic progress. This research validates the scientific rigor and practical utility of the proposed six-dimensional indicator framework as an evaluative tool, offering a model for the sustainable development and cultural preservation of similar regional heritage assets. [ABSTRACT FROM AUTHOR]

Snail-eating turtles (Malayemys spp.) are freshwater turtles native to mainland Southeast Asia. Three snail-eating turtle species (Malayemys khoratensis, Malayemys macrocephala, and Malayemys subtrijuga) were previously reported in Thailand. Before the present study, information on the distribution of snail-eating turtles in northeastern and eastern Thailand was limited, and a question arose regarding the distribution range of the new species, Malayemys khoratensis. From 2018 to 2020, we surveyed and collected data on the morphological traits of 109 snail-eating turtles along with 60 blood samples from 23 out of 27 provinces across northeastern and eastern Thailand. We found that both M. khoratensis and M. subtrijuga occur in northeastern Thailand. The distribution of M. khoratensis is in the Chi River Basin and upper Mun River Basin to the Mekong River. while the distribution of M. subtrijuga is from the lower Mun River to the Mekong River. Malayemys macrocephala occur in eastern Thailand and were found in 2 large provinces (Nakhon Ratchasima and Udon Thani) in northeastern Thailand. Based on morphological characteristics known to distinguish these species, 28 of the sampled individuals from areas in lower Mun River basin could not be assigned to any of the 3 species. Therefore, we reconstructed the phylogenetic relationships of 26 of these samples as well as other samples from known species based on 2 mitochondrial genes (Cyt b and ND4). Results based on mtDNA data indicated that the turtles in the unknown group belonged to 1 of the 3 clades corresponding to M. khoratensis, M. macrocephala, and M. subtrijuga. This result confirms that the unknown group does not represent a novel species within the genus Malayemys. However, it also indicates a discordance between the genetic and morphological characteristics of the turtles, highlighting the complexity of species identification within the genus Malayemys. [ABSTRACT FROM AUTHOR]

This study investigates the physiological responses of three grapevine cultivars to salt stress. Cuttings from cv. 'Sultana seedless', cv. 'Hamburg muscat' and the local variety cv. 'Ercis uzumu', were utilized. After rooting, the cuttings were brought to the vegetative stage with a single internode before subjecting them to salt treatments. Salt stress was induced using NaCl solutions at concentrations of 0, 50, and 100 mM at four-day intervals. Throughout the experiment, various physiological parameters were assessed, including ion homeostasis, osmotic regulation, antioxidant activity, and mineral uptake in different plant tissues. Our findings revealed significant variations in salt tolerance among the tested grapevine cultivars. Specifically, cv. 'Ercis uzumu' exhibited robust physiological responses to salt stress, indicating high tolerance, while cv. 'Sultana seedless' demonstrated moderate responses, and cv. 'Hamburg muscat' displayed heightened sensitivity to salt stress at the physiological level. [ABSTRACT FROM AUTHOR]

Leptochela Stimpson (1860) is a shallow-water, benthopelagic genus within the predominantly pelagic superfamily Pasiphaeoidea. We inventoried a global fauna of 17 currently valid species of Leptochela and identified a newly discovered eighteenth species. Our analysis combined both morphological and molecular data, using 13 characters (including two multistate characters) and 5 gene markers, respectively. The results revealed incongruence between the molecular and morphological datasets. However, our phylogenetic conclusions were based on a consensus approach, integrating morphological, molecular, and total evidence trees, which revealed three robust clades. We discuss the evolutionary development of quantitative and qualitative morphological traits in Leptochela and explore the potential causes of the incongruence between morphological and molecular signals, particularly in the context of pelagic eucarids transitioning from pelagic to benthopelagic habitats. Additionally, we describe the new species from Madagascar and provide a key to all known species of Leptochela. [ABSTRACT FROM AUTHOR]

The present research work reports the impact of multi-walled carbon nanotubes (MWCNTs) on sugarcane morphological characteristics, the production of secondary metabolite (SMs), and the antimicrobial activity of sugarcane callus culture in vitro. Explants (young leaf sheaths and leaf rolls) of usually smaller diameter were cultured on Murashige and Skoog (MS) medium following our previous protocol for sugarcane callogenesis. The induced calli were subjected to varying concentrations (1, 2, 3, 4, and 5 mg/l) of MWCNTs for multiplication. The highest multiplication was recorded on media containing 5.0 mg/l of CNTs with fresh weight 2.8567 g. Different concentrations (0, 5, 10, 15 and 20 mg/l) of MWCNTs were applied to regenerate the calli cultures. The highest number of shoots and roots were developed on 15.0 mg/l, while the highest shoot and root length (in centimeter) were observed on the highest used concentration, i.e. 20.0 mg/l CNTs. Similarly, the sub-cultured calli were subjected to phytochemical analysis and the effect of MWCNTs on amount of biochemical contents like total phenolic contents, flavonoids, saponins and terpenoids was determined. The highest contents were found in the culture containing the highest concentration of CNTs, in all cases. Moreover, the antioxidant activities of the MWCNTs induced in vitro sugarcane were also evaluated by the 1,1 diphenyl 2, picryl hydrazyl (DPPH) methods, which was found highest, i.e. 95.556% with EC50 value of 34.49 µg/ml for crude extract of callus grown on MS-media containing 5.0 mg/l MWCNTs. The antimicrobial activity was evaluated against two bacterial pathogens, Clavibactor michiganensis subsp. michiganensis (CMM) and Ralstonia solanacearum (RS) with inhibition zone of 14 mm in case of CMM, while 17 mm was measured against RS Spp. Our results reveal that using MWCNTs in specific concentrations could act as a plant growth promotor and novel elicitor for the in vitro biosynthesis of useful SMs with antimicrobial activity against broad spectrum microbial pathogens. [ABSTRACT FROM AUTHOR]

Determination of the distinctness, uniformity and stability (DUS) of plant cultivars is an important means of protecting the original innovations of breeders, increasing the enthusiasm of breeders and promoting the development of plant breeding. In this study, combinations of simple sequence repeat (SSR) markers and DUS morphological characteristics were used to identify cultivars. The genetic diversity of 30 flax plant cultivars was analyzed using 20 SSR markers and 18 DUS morphological characteristics. The results of the morphological characteristic analysis showed that the 30 flax cultivars had high genetic diversity. Ninety allelic variations were detected in 20 polymorphic SSR molecular markers, and 2–10 allelic variations were detected in each pair of primers. The diversity index (H) of Nei's gene range was 0.10–0.39, with a mean value of 0.23; the Shannon information index (I) range was 0.19–0.57, with a mean value of 0.37; and the polymorphism information content (PIC) range was 0.25–0.82, with a mean value of 0.51. Cluster analysis revealed that there were correlations between the SSR markers and morphological characteristics, and there were also some differences. These results show that these two methods have good potential for future research, especially for DUS analysis of hundreds of cultivars. Therefore, the use of molecular markers can reduce the cost and duration of resource consumption, and more importantly, identify and protect highly specific plant cultivars. [ABSTRACT FROM AUTHOR]

Despite the wide variety of knitting patterns present in the global market for synthetic meshes, the influence of mesh morphology on the mechanical behaviour of these devices is not yet clear. The goal of this project is to determine how the mechanical properties of monofilament polypropylene warp-knitted meshes are influenced by their morphological characteristics. Multivariate linear regression models were developed using nine meshes to quantify a correlation between these two crucial aspects. Additionally, porous mesoscale Finite Element (FE) models were implemented in Abaqus to replicate in vitro tests conducted in a previous study. High values of the determination coefficient were obtained for all the models and their robustness was confirmed by good performance in a leave one out validation method. The porous FE models successfully replicated the multidirectional behaviour of the meshes after the calibration of the elastic modulus based on the uniaxial tensile test. This combined approach demonstrates valuable results paving the way for advancements in hernia treatment and meshes design. [ABSTRACT FROM AUTHOR]

Proportions of positive and negative cloud-to-ground (CG) natural lightning are approximately 10% and 90%, respectively. Although the frequency of positive CG lightning is relatively low, these events are more likely to produce high current peaks compared to negative CG lightning. Additionally, positive CG flashes have longer continuous current durations and release larger amounts of charge, which can easily result in more severe disasters and accidents. Besides differences in leader polarity and occurrence frequency, there are also differences in morphological characteristics of channel development between positive and negative CG lightning. Moreover, morphological differences between positive and negative CG flashes can result in varying probabilities of side-flashes on tall buildings. However, because of the infrequent occurrence of positive CG flashes, current research on the morphological characteristics of lightning channel development remains quite limited. There remains a lack of clarity concerning the morphological characteristics associated with the development of positive CG lightning channels, as well as the morphological differences between positive and negative CG lightning channels.Based on a collection of digital images of 50 CG lightning events (25 positive and 25 negative), the optical two-dimensional morphological characteristics of lightning channels with different polarities are investigated and compared. Results indicate that there are significant differences in the number of bifurcations between positive and negative CG lightning channels. Negative CG lightning channels display a greater number of bifurcations, ranging from 7 to 85, with an average of 35. In contrast, 76% (19/25) of positive CG lightning cases only observed the return stroke channel, without any additional bifurcations. Except for one instance involving 42 bifurcations, the number of bifurcations in other positive CG lightning events does not exceed six. This difference in bifurcation number also leads to differences in fractal dimension, with the fractal dimension of positive CG lightning ranging from 1.03 to 1.30 (average of 1.11), while that of negative CG lightning has an average of 1.21, ranging from 1.12 to 1.30, with its minimum value exceeding the average of positive CG lightning. Moreover, there are significant differences in the tilt angles and directional turning counts of the return stroke channels between positive and negative CG lightning. Compared to negative CG lightning, the return stroke channels of positive CG lightning tend to develop more inclinedly with fewer directional changes.There is no significant difference in relative length (the ratio of the straight-line distance from the cloud exit point of the return stroke channel to the strike point to the total length of the return stroke channel) of return stroke channels between downward positive and negative cloud-to-ground lightning.

The present study aimed to evaluate and analyse the variation of morphological parameters in garlic according to genotype and crop year, to obtain valuable insights for identifying genotypes with superior performance under specific environmental or cultivation conditions. Fifteen autumn garlic genotypes selected from the Oltenia region, Romania, and the registered 'Benone' variety, cultivated in the Craiova area, were evaluated over two consecutive years (2022 and 2023). The study's results revealed significant differences among genotypes for all analysed traits (bulb weight, bulb size, number of cloves), highlighting the influence of both genotype and year on these characteristics. Notably, genotype M15 exhibited the highest bulb weight (49 g). Local genotypes outperformed the registered variety, underscoring the superiority of the selected genotypes and their value for breeding or cultivation purposes. These findings provide important insights for selecting garlic genotypes based on specific environmental conditions and cultivation requirements. The information obtained may contribute to improving garlic production and quality in the Oltenia region, as well as promoting genetic diversity and crop sustainability.

Megalobrama amblycephala, which belongs to the genus Megalobrama of the subfamily Culterinae in the family Cyprinidae, is an important freshwater cultured species in China because of its low culture cost and high growth rate. Culter alburnus, belonging to the subfamily Culter, is another slender carnivorous species, whose meat is more delicate and delicious than that of M. amblycephala; however, the scales of C. alburnus are small and thin and are easily injured and fall off, and these fish are slow-growing and expensive to feed. Although both the fish are quite different in terms of diet, growth, and stress resistance, they are highly complementary to each other. Crossbreeding can combine the desired characteristics of both parents; however, this advantage is usually limited to the F1 generation. Theoretically, because the gonads are almost undeveloped during triploid growth and development, F1 heterosis can be maintained to avoid germplasm decline caused by continuous reproduction, and energy can be saved to accelerate growth and improve muscle quality. This will greatly expand the breeding space of new hybrid varieties and ensure high economic, social, and ecological value. Therefore, the triploids of M. amblycephala ♀ × C. alburnus ♂ have superior growth and economic value. To understand the growth and morphological characteristics of the triploids of M. amblycephala ♀ × C. alburnus ♂, the triploid population of M. amblycephala ♀ × C. alburnu ♂ was induced by hydrostatic pressure, and successfully induced triploid (hybrid-3n) and uninduced diploid (hybrid-2n) populations were obtained. At the same time, two self-breeding populations of Megalobrama (MA) and Culter (CA) were established. The growth performance and morphological characteristics of the four kinds of fish were compared and analyzed in growth contrast experiments. The results showed that during the 210-day growth cycle, the absolute weight gain (0.88±0.11 g/day) of hybrid-3n was 8.64% higher than that of hybrid-2n, 20.55% higher than that of MA, and 120.00% higher than that of CA. In terms of countable traits, the hybrid index of hybrid-3n and hybrid-2n was 41.05 and 36.07, respectively. In terms of measurable traits, the hybrid index of hybrid-3n and hybrid-2n was 36.73 and 57.57, respectively. Cluster analysis showed that hybrid-3n was first grouped with hybrid-2n, then grouped with maternal MA, and finally grouped with paternal CA. The results showed that hybrid-3n and hybrid-2n were closer to their mothers in quantifiable traits and frame structure, showing a maternal effect. Discriminant analysis showed that the discriminant accuracy of hybrid-3n was 90%, and the comprehensive discriminant rate of the four populations was 95%. A scatterplot of the discriminant analysis showed that the distribution centers of hybrid-3n and hybrid-2n were located between the parents and closer to the mothers. Hybrid-3n and hybrid-2n occupied partially overlapping areas, which indicates that the two are similar in proportion and frame structure, making it difficult to distinguish between them. These results are basically consistent with those obtained by cluster analysis. Most of the 11 biological traits used to construct the discriminant function were related to the longitudinal axis of the body, especially the ratio of the height of the body to the trunk, which is similar to the results obtained by principal component analysis. During principal component analysis, four principal components with a cumulative contribution rate of 75.10% were obtained, which mainly reflect morphological variations of body height and trunk length. In this study, the experimental fish were cultured in still water ponds with abundant bait. The morphological differences may be attributed to the adaptation of the fish to this ecological environment. Comprehensive analysis showed that: Hybrid-3n has a fast growth rate and has the basic conditions for promotion and application in production; The body size of hybrid-3n and hybrid-2n is between the parents, and both are slightly biased towards the mother, which mainly reflect the morphological variations of body height and trunk length; In this study, three multivariate analyses were used to effectively reflect the morphological differences among the four populations of hybrid-3n, hybrid-2n, MA, and CA from different perspectives, which has made them irreplaceable. In conclusion, this study confirmed the superior breeding potential of triploids of M. amblycephala ♀ × C. alburnu ♂, and provided basic data for the morphological comparison of hybrid offspring of M. amblycephala and C. alburnu, which is significant for the identification and protection of fish resources, and is expected to provide a theoretical basis for the establishment of new strains of M. amblycephala and C. alburnu. The results are of great significance for population identification and ploidy breeding of hybrid offspring of M. amblycephala and C. alburnu.

Cover crops, typically planted during off-seasons and requiring less agronomic manipulation, may provide abundant fungal resources. Certain species of Chaetomiaceae could serve as potential agents for controlling plant diseases and developing bioorganic fertilizers. Eight species from five genera of Chaetomiaceae were identified from healthy Astragalus sinicus and Vicia villosa, two major cover crops, through multigene phylogenetic analysis, morphological identification, and pairwise homoplasy index testing. The identified species comprise three new species: Achaetomium astragali, Subramaniula henanensis, and S. sichuanensis, as well as five known but new host record species: Botryotrichum murorum, Chaetomium coarctatum, C. pseudocochliodes, C. pseudoglobosum, and Collariella pachypodioides. Dual culture tests revealed that isolates of all eight Chaetomiaceae species exhibited antagonistic effects on multiple phytopathogens. Among the identified fungi, the NSJA2 isolate, belonging to C. coarctatum, exhibited significant relative inhibition effects on 14 out of 15 phytopathogens tested in this study, indicating its broad-spectrum antagonistic effects. Additionally, NSJA2 exhibited excellent salt tolerance. Overall, our study has identified multiple fungi with anti-phytopathogens potential, among which NSJA2 exhibits high potential for practical application. This finding paves the way for further exploration and exploitation of NSJA2 as a promising biocontrol agent. [ABSTRACT FROM AUTHOR]

Moderate deficit irrigation can improve lignin metabolism, thereby increasing wheat yield and lodging resistance. The moisture-sensitive variety Xinchun 22 (XC22) and drought-resistant variety Xinchun 6 (XC6) were used as experimental materials. We set mild drought (T1, J1 and 60–65% FC, where FC is the field capacity) and moderate drought (T2, J2 and 45–50% FC) during the tillering stage (T) and the jointing stage (J). We used conventional drip irrigation as a control (CK and 75–80% FC). The results show that the activity of lignin synthesis-related enzymes decreased with the growth process, while the accumulation and monomer content of lignin increased under different water treatments. The lignin metabolism and morphological characteristics of XC6 were higher than those of XC22. Under the same processing conditions, the indicators of XC22 showed more significant changes and were more sensitive to changes in the moisture content. Compared with other treatments, the stem thickness and wall thickness of the J1 treatment increased by 0.86–23.49% and 1.72–23.58%. The yield of the T1 treatment was the highest, increasing by 3.05–44.06% compared to other treatments. In addition, by improving PAL, H-type lignin monomers, S-type lignin monomers, stem thickness and lignin metabolism, grain yield can be increased. After mild drought during the jointing stage, J1 significantly improved the lignin metabolism capacity of the stem, increased stem thickness and wall thickness, and was beneficial for improving lodging resistance. The T1 treatment favored the improvement of the production capacity of assimilates, thus promoting a high yield of spring wheat. [ABSTRACT FROM AUTHOR]

Drought is one of the major abiotic stresses that affect plant growth and productivity, and plant stress responses are affected by both the intensity of stress and genotype. In Iraqi Kurdistan, tomato plants play a significant role in the country's economy. Due to climate change, which causes soil moisture to diminish, the crop's growth and yield have been dropping in recent years. Accordingly, the effects of simulated drought stress on germination parameters were assessed in 64 tomato accessions gathered from the Iraqi Kurdistan region in order to identify sensitive and tolerant accessions. In this respect, the responses associated with drought stress were observed phenotypically and biochemically. Germination percentage (GP) and morphological characteristics such as root length (RL), shoot length (SL), and shoot fresh weight (SFW) were significantly reduced in both stress treatments with polyethylene glycol (PEG-6000) (7.5% PEG and 15% PEG). On the other hand, significant changes in biochemical profiles such as proline content (PC), soluble sugar content (SSC), total phenolic content (TPC), antioxidant activity (AC), guaiacol peroxidase (GPA), catalase (CAT), and lipid peroxidation (LP) in tomato accessions were detected; all biochemical traits were increased in most tomato accessions under the PEG-induced treatments compared to the control treatment (0.0% PEG). Three tomato accessions (AC61 (Raza Pashayi), AC9 (Wrdi Be Tow), and AC63 (Sandra)) were found to be the most tolerant accessions under all drought conditions, whereas the performances of the other tested accessions (AC13 (Braw), AC30 (Yadgar), and AC8 (Israili)) were inferior. The OMIC analysis identified the biomarker parameters for differentiating the highly, moderately, and low tolerant groups as PC, SSC, and TPC. This study shows that early PEG-6000 screening for drought stress may help in choosing a genotype that is suitable for growth in water-stressed environments. Hence, Raza Pashayi, Wrdi Be Tow, and Sandra accessions, which had great performances under drought conditions, can be candidates for selection in a breeding program to improve the growth of plants and production in the areas that face water limits. [ABSTRACT FROM AUTHOR]

During the observation of a garlic plantation, a sample consisting of plants with symptoms of growth inhibition and the soil surrounding the roots was collected. Phytophagous nematodes were isolated from the soil sample as well as the garlic plant shoots, with an average density of 75 individuals per 100 mL. Microscope slides were prepared from adult individuals and subjected to morphological and morphometric analysis, as well as DNA examination. It was demonstrated that the examined nematodes belong to the species Robustodorus subtenuis. Both the female and male nematodes differed from individuals previously found in the cultivation of Narcissus and Gladiolus. On average, R. subtenuis females have shorter bodies and longer tails, with the averages calculated for the nematode population from the soil being slightly lower than the average values for nematodes from the garlic shoots. Additionally, the females found in the soil had a shorter distance from the lip region to the vulva and a shorter post-vulval sac, the length of which constituted 32 to 45% of the distance from the vulva to the anus. On average, the male bodies were shorter and had longer tails. They were also wider at the level of the anus. The average body length of males isolated from the soil, like that of the females, was lower than the average for individuals isolated from the garlic shoots. Variations in the influence of the host plant on the morphometry of the parasitic nematodes were observed. This is the first report of R. subtenuis colonizing garlic plants in Poland. [ABSTRACT FROM AUTHOR]

Cement plays a crucial role in mortar composition, and its particles exhibit a strong tendency to flocculate when mixed with water. This flocculation necessitates the addition of substantial water to improve the workability of mortar or concrete. However, this extra water does not contribute to the hydration reaction, potentially weakening the mechanical properties of the mortar. The introduction of superplasticizers has addressed this issue by reducing the water demand. Nevertheless, a common practice in construction is to use a standard dosage of superplasticizers for the same type of cement, even when sourced from different cement factories, which may affect material performance. This study investigated the influence of varying superplasticizer dosages on Portland pozzolana cement from different Ethiopian cement factories, examining the morphological characteristics of masonry mortar. Specifically, it evaluated the impact on cracks, porosity, and surface structure across different curing ages, as well as assessed the mortar's physical and mechanical properties. Mortar samples prepared using cement from three factories, labeled A, B, and C, with constant cement‐to‐sand ratios of 1:3 and water‐to‐cement ratios of 0.5. Superplasticizer added in varying amounts 0%, 0.6%, 0.8%, and 1%. The morphological analysis conducted using a 3D optical surface profiler microscope at 1, 7, 21, and 28 days. Besides these standard physical and mechanical tests also performed on all samples. The results demonstrated that addition of superplasticizers significantly influenced the surface morphology of mortar samples. Mortars A and B exhibited denser structures with superplasticizer dosages 0.8% and 1%, respectively, whereas mortar C displayed a denser structure in its control state (without superplasticizer). The flexural and compressive strength tests also revealed notable differences. Sample A2 (0.8%) from group A, sample B3 (1%) from group B, and sample C0 (0%) from group C achieved the highest strength within their respective groups at 28 days. The findings suggest that it is essential to evaluate the specific properties of cement before applying a standardized superplasticizer dosage, as variations in cement production can significantly influence the performance of the mortar. [ABSTRACT FROM AUTHOR]

Carrot (Daucus carota L.) is a globally significant root vegetable, rich in bioactive and nutritional compounds. Carrot is a crop with a wide range of phenotypic variability including colour, shape, size, and other characteristics. The aim of this work was to determine and compare selected morphological traits and chemical composition of 24 carrot varieties grown under open field conditions in Slovakia. Altogether, 11 qualitative characteristics and 6 quantitative parameters were evaluated. The carrot roots were also analysed for essential chemical constituents including reducing sugars (fructose, glucose, saccharose), carotenoids, dietary fibre, dry matter, ash, and mineral compounds. A wide range of diversity in morphological characteristics and chemical composition indicates a high variability of studied varieties. The root length ranged from 59 to 205 mm, root diameter varied from 17.7 to 39.0 mm and root weight ranged from 21.0 to 74.8 g. Based on the measurement results, saccharose was the dominant sugar ranging from 1.89 to 4.67 g.100 g-1 of fresh weight, carotenoid content varied from 85.94 to 374.24 µg.g-1 of fresh weight, the amount of dietary fibre ranged from 25.67 to 36.02% of dry matter. Considerable differences were also observed in the content of macro and microelements [mg.100 g-1] ranging as follows: P (17.5 - 52.0), K (110.0 - 337.0), Na (65.9 - 186.0), Fe (0.324 - 3.340), Cu (0.042 - 0.126), Zn (0.152 - 0.945) and Mn (0.062 - 0.189). The presented results can provide useful information for carrot growers and consumers in terms of morphological characteristics as well as the chemical composition of individual carrot varieties. [ABSTRACT FROM AUTHOR]

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An investigation into the spatial–temporal distribution characteristics of ancient settlements and their relationship with the environment in the Sichuan Basin can enhance our understanding of the evolution of the regional human–land relationship. Utilizing kernel density analysis by GIS, this paper obtained the spatial distribution characteristics of 1100 settlements in the Neolithic, Shang–Zhou, and Qin to Southern Dynasties periods. Subsequently, a GIS spatial analysis was performed to examine the distribution of these settlements in relation to the natural environment, focusing on four aspects: elevation, slope, aspect, and water buffer zone. The results demonstrate that: (1) The distribution of settlements from the Neolithic to the Southern Dynasties has expanded from the Chengdu Plain in the west and the Xiajiang Area in the east to the hilly areas in the center. (2) The settlements in the Neolithic and Shang–Zhou periods are predominantly located at elevations between 300–600 m, slopes between 0–3°, aspects between 135–225°, distances to rivers between 0–1000 m, and soil choice on Luvisols, Cambisols, Regosols, and Anthrosols. The influence of aspect on settlements during the Qin–Southern Dynasties period was notably reduced. The settlements mainly grow in the Chengdu Plain and the smaller plains along the river from the Neolithic to the Southern Dynasties in the Sichuan Basin. (3) The emergence and advancement of primitive agriculture, the construction of early water conservancy facilities, commercial and trade exchanges, and the establishment of regimes led to a shift from a double core to a more dispersed distribution in the Sichuan Basin from the Neolithic to the Southern Dynasties periods. The evolution of settlements reflects the adaption and competition of the residents to the natural environment in the Sichuan Basin. Furthermore, it provides insights into the complex social development in the region, ranging from the formation of early states to the establishment of the Qin and Han Empires. [ABSTRACT FROM AUTHOR]

The present study aims to document the traditional knowledge and the distribution of diverse halophytes used by the coastal community of Vietnam to compensate for the food, fodder, and medicine demands. The information was gathered from the local people, plant traders, and herbalists using a semi-structured questionnaire. The survey indicated the ethnobotanical uses of 11 plant species from 8 families in the targeted area. 60% of studied plants were used in more than two different ways in which the majority of halophytic species possessed medicinal values, followed by being culinary, fodder, roof thatching, handicraft, and growing medium. The main diseases in the regions were joint and stomach pain. According to the mode of preparation, decoction form was the predominance with 64% over all modes. Based on the result, Cocos nucifera L., Portulaca oleracea L., and Suaeda maritima (L.) Dumort. had the highest appearance frequency of 17%. In addition, the seashore was the most prevalent location of halophytes that it was inhabited by 5 out of 11 species. The predominant habit recorded was herbs, followed by shrubs, trees, and vines. [ABSTRACT FROM AUTHOR]

Cyst nematodes are obligate parasitic nematodes found in the fields of many cultivated crops. These nematodes, which have great economic importance, pose a threat to food security, though they are frequently ignored or misdiagnosed as pests because of covert parasitism. A cyst nematode population parasitizing on Artemisia lavandulaefolia DC., one of the traditional Chinese medicines was collected in Gansu Province. The species was diagnosed using integrative taxonomy and molecular approaches. The cyst population is spherical or lemon-shaped, light brown or dark brown in color, with a long neck and a protruding vulval cone. The stylet of the second-stage juvenile is strong, and the front end of the ball at the base of the stylet is concave; the median bulb and excretory pore are prominent; the tail is blunt and circular, and the transparent tail is usually shorter than the stylet. A phylogenetic analysis was carried out using the internal transcribed spacer (ITS) and 28S genes of ribosomal DNA, which further confirmed the presence of Cactodera chenopodiae. According to our literature review, this is the first report on C. chenopodiae in Compositae. By following this research, we can better understand the challenges posed by A. lavandulaefolia DC. and develop effective strategies for managing its spread and impacts. This will help to protect vulnerable ecosystems and ensure the sustainability of agricultural and forestry activities in affected areas. [ABSTRACT FROM AUTHOR]

Migration of Sakhalin sculpin Cottus amblystomopsis larvae from the river to the sea for early rearing—the primary dispersion, occurs in the form of passive downstream migration in the first 1.0–1.5 h after dark. Reaction of larvae to decrease of illumination ensures their involvement into the flow and the implementation of downstream migration. Total duration of primary dispersion period does not exceed 3 days. Body length of the larvae is on average 7.7 mm, their vertebrae and spinous processes are formed, the anlages of unpaired fins are noticeable, and pectoral fins are well developed. [ABSTRACT FROM AUTHOR]

This study presents a comprehensive analysis of the vascular bundle morphology, tangential and radial diameters, and distribution frequency of different parts of Oligostachyum sulcatum, elucidating their structural and functional significance. Electron microscopy images revealed distinct vascular bundle characteristics in the different parts, including the vascular bundles in both parts of the rhizomes, the middle parts of the internodes, and the middle and inner parts of the branches, which were semi-open. The vascular bundles in the outer parts of both internodes and branches were semi-differentiated and undifferentiated. The vascular bundles in the inner parts of internodes were open. Statistical analysis showed significant variations in tangential and radial diameters among these parts, reflecting their diverse mechanical and physiological functions. The internodes exhibited the largest tangential and radial diameters, suggesting a critical role in mechanical support. In contrast, the branches had the smallest diameter, indicating that vascular bundle differentiation is influenced by growth conditions. The vascular bundle frequency was the highest in branches and the lowest in inside-sticks. This study provides theoretical references for the adaptive strategies and growth regulation mechanisms of O. sulcatum. [ABSTRACT FROM AUTHOR]

Endocervical adenocarcinoma (ECA) is reported increasingly often in young women, and this aggressive disease lacks effective methods of targeted therapy. Since mismatch repair deficiency (dMMR) is an important biomarker for predicting response to immune checkpoint inhibitors, it is important to investigate the clinicopathological features and immune microenvironment of dMMR ECAs. We assessed 617 ECAs from representative tissue microarray sections, gathered clinicopathologic information, reviewed histological characteristics, and performed immunohistochemical staining for MMR, programmed cell death 1 (PD-L1), and other immune markers. Of 617 ECA samples, 20 (3.2%) cases had dMMR. Among them, loss of MMR-related proteins expression was observed in 17/562 (3.0%) human papilloma virus-associated (HPVA) adenocarcinoma and 3/55 (5.5%) non-HPV-associated (NHPVA) adenocarcinoma. In NHPVA cohort, dMMR status was observed in 3 (3/14, 15.0%) patients with clear cells. dMMR ECAs had a higher tendency to have a family history of cancer, larger tumor size, p16 negative, HPV E6/E7 mRNA in situ hybridization (HPV E6/E7 RNAscope) negative, and lower ki-67 index. Among the morphological variables evaluated, poor differentiation, necrosis, stromal tumor-infiltrating lymphocytes, peritumoral lymphocytes, and lymphoid follicles were easily recognized in the dMMR ECAs. In addition, dMMR ECAs had higher CD3+, CD8+, CD38+, CD68+ and PD-1+ immune cells. A relatively high prevalence of PD-L1 expression was observed in dMMR ECAs. dMMR ECAs were significantly more likely to present with a tumor-infiltrating lymphocytes -high/PD-L1-positive status. In conclusion, dMMR ECAs have some specific morphological features and a critical impact on the immune microenvironment, which may provide insights into improving responses to immunotherapy-included comprehensive treatment for ECAs in the future. [ABSTRACT FROM AUTHOR]

In this study, we have found two new species—Stropharia subrugosoannulata and Stropharia microaeruginosa. Phylogenetic analyses, based on the internal transcribed spacer regions (ITS) and the nuclear ribosomal RNA gene (nrLSU), suggest that the two new species are distinct and monophyletic. S. subrugosoannulata is distinguished from other species of the genus Stropharia by the pileus covered with greyish-orange squamules in the centre, stipe light brown and surface covered with white triangular squamules. S. microaeruginosa differs from other species in its pileus bluish-grey when young becoming lighter towards margins, later greyish-turquoise lightens towards the edges and surface radially striate when young, lamellae adnate to subdecurrent, stipe with white squamules at the base, acanthocytes absent. The new record species from Jilin Province, Clitolyophyllum umbilicatum was also confirmed, based on morphological and molecular study. Here, we have given full descriptions of each species, colour images, illustrations and two phylogenetic trees that show the placement and relationship of the two new species and the new record are provided. [ABSTRACT FROM AUTHOR]

Anthropometric characteristics and body composition are significant factors for success in elite football, and there has been a growing body of research on this subject recently. This study aimed to investigate changes in the anthropometric characteristics and body composition of football players during both preparatory and competitive periods. The research involved 10 professional football players (24.4±4.84 yrs.) competing in the First Montenegrin Telecom League. Measurements were taken in January before the preparatory phase, then again 30 days prior to the start of the season in February, and after the season in May. Anthropometric characteristics and body composition were evaluated using a set of 15 variables, including body height, body weight, triceps skinfold, biceps skinfold, back skinfold, abdominal skinfold, lower leg skinfold, upper leg skinfold, body mass index, fat kg, fat percentage, muscle mass kg, muscle mass percentage, lean body mass kg, lean body mass percentage. There were no differences in anthropometric characteristics across the three measurement periods for the football players. However, differences were observed in all six parameters of body composition across the three measurement periods. Fat mass and fat percentage significantly decreased after the competitive phase compared to measurements before and after the preparatory period. Furthermore, muscle mass, muscle mass percentage, lean muscle mass, and lean muscle mass percentage significantly increased in football players after the competitive season compared to measurements before and after the preparatory period. This indicates that the competitive season has a positive effect on changes in body composition, leading to a reduction in fat mass while simultaneously increasing muscle and lean mass components in football players, while anthropometric parameters remain unchanged. [ABSTRACT FROM AUTHOR]

This study aimed to determine differences in morphological characteristics and body composition between female handball players from the national teams of Montenegro and Kosovo (U16). Thirty-four participants were included in the study, divided into two subgroups. The first subgroup consisted of 18 players from the Montenegro national team, with an average age of 14.68±0.58 years. The second subgroup consisted of 16 players from the Kosovo national team, with an average age of 15.33±0.66 years. Testing was conducted during the qualification rounds for the European Championship took place in Podgorica in 2021. All players were assessed for anthropometric measurements and body composition, with a total of twelve variables. Body height, body weight, hand length, arm span, triceps skinfold, biceps skinfold, subscapular skinfold, abdominal skinfold, calf skinfold, thigh skinfold, body mass index (BMI), and body fat percentage (BF).The Tanita - model BC-418MA was utilized for the assessment of body composition. Differences in morphological characteristics and body composition of the female handball players from the two national teams were determined through discriminant parametric procedures using the T-test for independent samples with statistical significance set at p < .05. The results indicated that the players from the Montenegro team were significantly younger, had significantly greater average body height, body weight, and hand length compared to players from the Kosovo team. Statistical differences between the two treated teams were found in the variable thigh skinfold, while no significant differences were observed in BMI and BF. [ABSTRACT FROM AUTHOR]

In deep underground geotechnical engineering, water–rock interaction emerges as a critical factor influencing the stability of engineering rock masses. This study delves into the macroscopic nonlinear mechanical behavior and fracture characteristics of natural water-bearing and saturated sandstone samples under various true triaxial stress states. The objective is to systematically elucidate the impact of actual three-dimensional stress conditions and water content on the mechanical behavior and fracture mechanisms of sandstone formations. The findings reveal significant anisotropic deformation in sandstone under 3D stress, while saturated sandstone exhibits robust plastic deformation capacity. Moreover, changes in the strain ratio coefficient are identified as precursors to rock fracture. The dilatation behavior of sandstone weakens progressively with increasing σ2, indicating reduced expansion in saturated sandstone. To account for the influence of σ2, a novel dilation index is proposed. The true triaxial strength of sandstone demonstrates a close relationship with both σ2 and water content, with strength initially increasing before declining as σ2 rises. In addition, water significantly undermines sandstone strength, although this weakening trend moderates with increasing σ2. Morphological analysis and SEM observations unveil three-dimensional volume fracturing characteristics in rock fractures, primarily characterized by shear accompanied by tensile fractures. Saturated sandstone samples, in contrast, exhibit numerous tensile and intergranular fractures. Overall, this study holds paramount practical significance for the evaluation of rock stability and the design of water-bearing engineering rock masses, encompassing domains, such as geology, water conservancy, and mining. Highlights: The influence of the σ2 and water on the nonlinear mechanical behavior of sedimentary sandstone was studied. A new dilatation index and strength criterion considering the effect of the σ2 was proposed. The 3D fracture morphology and failure network characteristics of sandstone was analyzed. Mesoscopic fracture mechanism of the σ2 and water on sandstone was revealed. [ABSTRACT FROM AUTHOR]

Mechanized harvesting is a crucial step in the agricultural production of foxtail millet (Setaria italica), as its peduncles are susceptible to bending and breaking during the harvesting process, leading to yield losses and deterioration in grain quality. To evaluate the suitability of foxtail millet for mechanical harvesting, this study comprehensively analyzed the biomechanical properties of the peduncles and related biological morphological characteristics of 116 foxtail millet accessions, establishing a system for indicator screening and comprehensive evaluation. Using partial correlation analysis and R-type cluster analysis, four biomechanical and seven related morphological indices of the peduncle were screened from 22 candidate indicators, with their coefficient of variation ranging from 6% to 80%. The entropy method was used to assign weights to the selected indices, with biomechanical factors contributing 47.4%, peduncle morphology 20.2%, spike morphology 27.6%, and plant height 4.8%. The Technique for Order Preference by Similarity to Ideal Solution (TOPSIS) and Rank-Sum Ratio (RSR) methods were applied to rank and grade the classification of the 116 foxtail millet varieties into four performance groups: Excellent (8 varieties), Good (50 varieties), Moderate (51 varieties), and Poor (7 varieties). This study provides a scientific basis for the selection and evaluation of foxtail millet varieties. [ABSTRACT FROM AUTHOR]

The combustion and fragmentation characteristics of wet coal slime particle are different from that of dried coal particle. In this paper, an understanding of the combustion process of wet coal slime particle through the combined results of the morphology characteristics, mass changes, and gas release pattern is provided. A tube furnace and high-speed camera were used to observe morphological changes of the combustion process of wet coal slime. For comparison, the mass change and gas release pattern of wet coal slime was monitored by a thermogravimetric analyzer and mass spectrometer (TG/MS). The four combustion stages of coal slime were observed for the first time: (1) drying and evaporation, (2) devolatilization and fragmentation, (3) volatile homogeneous combustion, and (4) char heterogeneous combustion. The water content in wet coal slime greatly affected the fragmentation of coal slime, it could build up a great internal pressure when evaporated, which caused the primary fragmentation to occur earlier and be more severe than traditional coals. The temperature and diameter of the slime were considered as factors for the occurrence of fragmentation and the level of it. A larger diameter with a higher furnace temperature resulted in a more severe fragmentation of the wet coal slime. The smaller coal slime at the lower temperatures did not experience the fragmentation stage. Here, the fragmentation and non-fragmentation zones of wet coal slime at different temperatures and different paticle diameters are given, which could be a guide for the combustion of coal slime in CFB(Circulating Fluidized Bed). Also, for larger diameters and lower setting temperatures, the ignition delay time and burnout time of wet coal slime became longer. The gas release pattern of wet coal slime during the combustion process was analyzed, and it was determined that it could be affected greatly by fragmentation. A model of the drying and evaporation stage of wet coal slime was established for the first time by using COMSOL Multiphysics. The results of the theoretical calculations show that there were two evaporation rate peaks of water when fragmentation occurred, which theoretically explained the early stage of the combustion process of wet coal slime, and the results were in agreement with the experiments. [ABSTRACT FROM AUTHOR]

Determining the reference base of anthropometric parameters on a sample of elite athletes is one of the foundations of further research and forming a clearer picture of each sport and sports discipline. In this study, the aim was to describe the anthropometric and somatotype profiles of elite Finn class sailors and to determine the differences in the measured parameters between sailors at different levels of general competitive success. The subject sample included 57 Finn class sailors who competed at the open Finn European Championship. A set of 25 anthropometric variables were applied. The sailors were divided into three groups according to their level of general competitive success using World Sailing Rankings. Finn sailors had higher average values in almost all morphological characteristics when compared to the sailors in other Olympic classes. Considering the average values of somatotype categories, we determined that Finn sailors fit the endomorphic mesomorph somatotype category (3.94 ± 1.19 − 5.50 ± 1.19 − 1.63 ± 0.74). Significant differences were observed between more-successful, medium, and less-successful sailors in the variables of age, body mass, muscle mass, arm muscle mass, and endomorphy rating. These results indicate the possibility of selection processes and/or adaptation to sailing occurring in the Finn class. The anthropometric characteristics of Finn sailors compared to sailors in Olympic classes further "support" the Finn class being called the "heavy dinghy" male class. This study on anthropometric parameters, determined via a sample of top Finn sailors, may be of great help to coaches and young sailors when deciding on the selection of an adult sailing class. [ABSTRACT FROM AUTHOR]

ABSTRACT A new species of the genus Microcosmus was described in this study based on specimens collected from the coast of Xilian Town, Xuwen County, Zhanjiang, Guangdong Province, China. The morphological and molecular characteristics of this new species, Microcosmus sp. z YZ‐2024 (YZ‐2024), distinguish it from other sea squirts. Firstly, YZ‐2024 can be preliminarily distinguished by the following morphological features: (1) smooth surface of the tunica, without protuberances; (2) except for the attachment site, the tunica is orange‐red; (3) absence of neural ganglia, and the tunica does not contract abruptly when disturbed; (4) the heart is oval‐shaped. Most importantly, the mitochondrial genome characteristics of YZ‐2024 effectively and accurately distinguish it from other congeneric species, with a mitochondrial genome size of 14,520 bp (GenBank No. PP067884) and the proportions of bases A, T, G, and C comprising 26.83%, 47.16%, 16.91%, and 9.10%, respectively. Except for the gene COX1, the mitochondrial genome arrangement of YZ‐2024 is totally different from that of 22 other species in the class Ascidiacea. Evolutionary analysis has indicated that YZ‐2024 clusters with its congeneric species Microcosmus sulcatus. Interestingly, YZ‐2024 belongs to Pyuridae, but it clustered with 12 species of Styelidae into a clade. Based on this, it can be suggested that YZ‐2024 is a new species of sea squirt in the northern Beibu Gulf of the South China Sea. Moreover, this study is the first report of molecular identification of sea squirt species in the northern Beibu Gulf of the South China Sea.

During the observation of a garlic plantation, a sample consisting of plants with symptoms of growth inhibition and the soil surrounding the roots was collected. Phytophagous nematodes were isolated from the soil sample as well as the garlic plant shoots, with an average density of 75 individuals per 100 mL. Microscope slides were prepared from adult individuals and subjected to morphological and morphometric analysis, as well as DNA examination. It was demonstrated that the examined nematodes belong to the species Robustodorus subtenuis. Both the female and male nematodes differed from individuals previously found in the cultivation of Narcissus and Gladiolus. On average, R. subtenuis females have shorter bodies and longer tails, with the averages calculated for the nematode population from the soil being slightly lower than the average values for nematodes from the garlic shoots. Additionally, the females found in the soil had a shorter distance from the lip region to the vulva and a shorter post-vulval sac, the length of which constituted 32 to 45% of the distance from the vulva to the anus. On average, the male bodies were shorter and had longer tails. They were also wider at the level of the anus. The average body length of males isolated from the soil, like that of the females, was lower than the average for individuals isolated from the garlic shoots. Variations in the influence of the host plant on the morphometry of the parasitic nematodes were observed. This is the first report of R. subtenuis colonizing garlic plants in Poland.

Mealybug is a renowned pest known to attack agricultural products from the field to the post-harvest process, such as on the seed rhizomes of Curcuma aeruginosa. Therefore, this study aimed to examine and identify the species of mealybug on the seed rhizomes of C. aeruginosa based on morphological and molecular characteristics. Fifty mealybugs were collected from the seed rhizomes of C. aeruginosa in the storage room in Bogor (Indonesia) using a soft brush. They were transferred to new C. aeruginosa rhizomes without any other insects present. Morphological identification based on observation of mounted specimens of 10 female adults and six for molecular identification. The primer pair that amplified the mitochondrial cytochrome oxidase I (COI) gene was used to study the molecular characteristics and was continued with direct sequencing and sequence analysis. The results showed that the morphological characteristics of the mounted specimen were close to those of Pseudococcus jackbeardsleyi. Amplification of the COI gene yielded DNA bands measuring 490 base pairs (bp), while homology and phylogeny analysis confirmed the morphological identification. Based on BLAST analysis, the similarity of COI genes of mealybugs in this study was above 99% with other P. jackbeardsleyi. The study specimen was identified as P. jackbeardsleyi on the seed rhizomes of C. aeruginosa by comparing the morphological features of insect specimens and results of the species available in GeneBank. This result represented the first documented report about the presence of the species in storage.

Abstract Background Understanding the mechanism of male sterility is crucial for producing hybrid seeds and developing sterile germplasm resources. However, only a few cytoplasmic male sterility (CMS) lines of cotton have been produced due to several challenges, like inadequate variation of agronomic traits, incomplete sterility, weak resilience of restorer lines, and difficulty in combining strong dominance. Therefore, the morphological and cytological identification of CMS in cotton will facilitate hybrid breeding. Results Two F2 segregating populations of cotton were constructed from cytoplasmic male sterile lines (HaA and 01A, maternal) and restorer lines (HaR and 26R, paternal). Genetic analysis of these populations revealed a segregation ratio of 3:1 for fertile to sterile plants. Phenotypic analysis indicated no significant differences in traits of flower bud development between sterile and fertile plants. However, sterile plants exhibited smaller floral organs, shortened filament lengths, and anther atrophy on the flowering day in comparison with the fertile plants. When performed scanning electron microscopy (SEM), the two F2 populations revealed morphological variations in the anther epidermis. Cellular analysis showed no significant differences in pollen development before pollen maturation. Interestingly, between the pollen maturation and flowering stages, the tapetum layer of sterile plants degenerated prematurely, resulting in abnormal pollen grains and gradual pollen degradation. Conclusion The results of this study suggest that fertility-restoring genes are controlled by a single dominant gene. Sterile plants exhibit distinctive floral morphology, which is characterized by stamen atrophy and abnormal anthers. Pollen abortion occurs between pollen maturity and flowering, indicating that premature tapetum degradation may be the primary cause of pollen abortion. Overall, our study provides a theoretical basis for utilizing CMS in hybrid breeding and in-depth investigation of the dominant configuration of cotton hybrid combinations, mechanisms of sterility, and the role of sterile and restorer genes.