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4 results on '"interlaboratory assay"'

1. Multicentre interlaboratory analysis of routine susceptibility testing with a challenge panel of resistant strains

Author

Corentin Deckers, Reza Soleimani, Olivier Denis, Pierre Bogaerts, Catherine Berhin, Hector Rodríguez-Villalobos, Julie Descy, Marie Hallin, Claire Nonhoff, Stefanie Desmet, Koen Magerman, Anne Marie Van den Abeele, Bénédicte Lissoir, Veerle Matheeussen, Kris Vernelen, and Te-Din Huang

Subjects
Interlaboratory assay, Quality control, Multidrug-resistant organisms, Automated susceptibility testing, Disk diffusion, Microbiology, QR1-502
Abstract

ABSTRACT: Objectives: In order to elaborate a new national challenge panel of resistant Gram-negative bacilli and Gram-positive cocci strains for the validation of routine antimicrobial susceptibility testing (AST) methods, an interlaboratory evaluation was organised. Methods: The results of 12 well-characterised multidrug-resistant strains tested by nine laboratories using local disk diffusion (DD) and automated AST (AUST) methods were compared with the reference broth microdilution method. Results: Overall categorical agreement ranged from 70% to 100% both for DD and AUST and was >90% for all but one strain for all antibiotics. Conclusion: Our multicentre AST study showed good reproducibility and the panel can be used as national resistant reference strains for routine AST validation.

Published
2022
Full Text
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2. Interlaboratory evaluation of Mucorales PCR assays for testing serum specimens: A study by the fungal PCR Initiative and the Modimucor study group.

Author

Rocchi, S, Scherer, E, Mengoli, C, Alanio, A, Botterel, F, Bougnoux, M E, Bretagne, S, Cogliati, M, Cornu, M, Dalle, F, Damiani, C, Denis, J, Fuchs, S, Gits-Muselli, M, Hagen, F, Halliday, C, Hare, R, Iriart, X, Klaassen, C, and Lackner, M

Abstract

Interlaboratory evaluations of Mucorales qPCR assays were developed to assess the reproducibility and performance of methods currently used. The participants comprised 12 laboratories from French university hospitals (nine of them participating in the Modimucor study) and 11 laboratories participating in the Fungal PCR Initiative. For panel 1, three sera were each spiked with DNA from three different species (Rhizomucor pusillus , Lichtheimia corymbifera, Rhizopus oryzae). For panel 2, six sera with three concentrations of R. pusillus and L. corymbifera (1, 10, and 100 genomes/ml) were prepared. Each panel included a blind negative-control serum. A form was distributed with each panel to collect results and required technical information, including DNA extraction method, sample volume used, DNA elution volume, qPCR method, qPCR template input volume, qPCR total reaction volume, qPCR platform, and qPCR reagents used. For panel 1, assessing 18 different protocols, qualitative results (positive or negative) were correct in 97% of cases (70/72). A very low interlaboratory variability in Cq values (SD = 1.89 cycles) were observed. For panel 2 assessing 26 different protocols, the detection rates were high (77–100%) for 5/6 of spiked serum. There was a significant association between the qPCR platform and performance. However, certain technical steps and optimal combinations of factors may also impact performance. The good reproducibility and performance demonstrated in this study support the use of Mucorales qPCR as part of the diagnostic strategy for mucormycosis. [ABSTRACT FROM AUTHOR]

Published
2021
Full Text
View/download PDF

3. Multicentre interlaboratory analysis of routine susceptibility testing with a challenge panel of resistant strains

Author

Deckers, Corentin, Soleimani, Reza, Denis, Olivier, Bogaerts, Pierre, BERHIN, Catherine, Rodríguez-Villalobos, Hector, Descy, Julie, Hallin, Marie, Nonhoff, Claire, Desmet, Stefanie, Magerman, Koen, Vanden Abeele, Anne Marie, Lissoir, Bénédicte, Matheeussen, Veerle, Vernelen, Kris, Huang, Te-Din, Belgian National Antibiogram Committee, Rodriguez-Villalobos, Hector/0000-0003-3041-1591, DECKERS, Corentin/0000-0003-2421-6669, Matheeussen, Veerle/0000-0003-2137-2068, Bogaerts, Pierre, Soleimani, Reza, Vernelen, Kris, Descy, Julie, Rodriguez-Villalobos, Hector, Nonhoff, Claire, MAGERMAN, Koen, Matheeussen, Veerle, Denis, Olivier, Van den Abeele , Anne Marie, Lissoir, Benedicte, Huang , Te-Din, Hallin, Marie, Deckers, Corentin, Berhin, Catherine, DE SMET, Stefanie, UCL - SSS/IREC/MONT - Pôle Mont Godinne, UCL - (MGD) Laboratoire de biologie clinique, UCL - SSS/IREC/MBLG - Pôle de Microbiologie médicale, UCL - (SLuc) Service de microbiologie, and Belgian National Antibiogram Committee

Subjects
disk diffusion, Microbiology (medical), Multidrug-resistant organisms, automated susceptibility testing, Pharmacology. Therapy, Immunology, Reproducibility of Results, Quality control, Microbial Sensitivity Tests, digestive system, Microbiology, digestive system diseases, QR1-502, Anti-Bacterial Agents, Interlaboratory assay, Anti-Infective Agents, Gram-Negative Bacteria, Immunology and Allergy, Disk diffusion, quality control, Automated susceptibility testing, multidrug-resistant organisms
Abstract

Objectives: In order to elaborate a new national challenge panel of resistant Gram-negative bacilli and Gram-positive cocci strains for the validation of routine antimicrobial susceptibility testing (AST) methods, an interlaboratory evaluation was organised. Methods: The results of 12 well-characterised multidrug-resistant strains tested by nine laboratories using local disk diffusion (DD) and automated AST (AUST) methods were compared with the reference broth microdilution method. Results: Overall categorical agreement ranged from 70% to 100% both for DD and AUST and was >90% for all but one strain for all antibiotics. Conclusion: Our multicentre AST study showed good reproducibility and the panel can be used as national resistant reference strains for routine AST validation. (C) 2022 The Author(s). Published by Elsevier Ltd on behalf of International Society for Antimicrobial Chemotherapy. The Belgian national reference centre is supported in part by the Belgian Ministry of Social Affairs through a fund within the national health insurance system (INAMI-RIZIV). The authors thank the members of the Belgian National Antimicrobial Susceptibility Testing Committee (NAC): Olivier Denis, Stefanie Desmet, Youri Glupczynski, Marie Hallin, Te-Din Huang, Bénédicte Lissoir, Koen Magerman, Veerle Matheeussen, Pierrette Melin, Karl Mertens, Hector Rodriguez, Anne-Marie Van Den Abeele and Kris Vernelen. The authors also thank Audric Deckers for his help in compiling the statistics for this article.

Published
2022

4. Interlaboratory evaluation of Mucorales PCR assays for testing serum specimens: a study by the fungal PCR Initiative and the Modimucor study group

Author

Rosemary Ann Barnes, J.P. Donnelly, Valérie Letscher-Bru, S Fuchs, Emeline Scherer, Maud Gits-Muselli, Birgit Willinger, Boualem Sendid, Massimo Cogliati, Ferry Hagen, R Hare, Stéphane Bretagne, Carlo Mengoli, Steffi Rocchi, Martina Lengerová, Michaela Lackner, Julie Denis, J. Loeffler, Alexandre Alanio, Wilfried Posch, P. L. White, Jan Springer, Laurence Millon, Françoise Botterel, Frédéric Dalle, Marie-Elisabeth Bougnoux, Xavier Iriart, C Klaassen, Céline Nourrisson, Céline Damiani, Florent Morio, Marjorie Cornu, Catriona Halliday, Mario Cruciani, Service de parasitologie et mycologie [CHRU de Besançon], Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon), Laboratoire Chrono-environnement - CNRS - UBFC (UMR 6249) (LCE), Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Unité de Glycobiologie Structurale et Fonctionnelle (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Laboratoire de parasitologie mycologie (CHU de Dijon), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), Vin Aliment Microbiologie et Stress (VAlMiS), Procédés Alimentaires et Microbiologiques (PAM), Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Procédés Alimentaires et Microbiologiques [Dijon] (PAM), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté [COMUE] (UBFC)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Laboratoire de parasitologie et de mycologie médicales [CHU Amiens], CHU Amiens-Picardie, Agents infectieux, résistance et chimiothérapie - UR UPJV 4294 (AGIR ), Université de Picardie Jules Verne (UPJV)-CHU Amiens-Picardie, Laboratoire de Parasitologie et de Mycologie Médicale [Strasbourg], Les Hôpitaux Universitaires de Strasbourg (HUS), Service de Parasitologie et Mycologie, CHU Toulouse [Toulouse]-Institut Fédératif de Biologie (IFB) - Hôpital Purpan, Hôpital Purpan [Toulouse], CHU Toulouse [Toulouse]-CHU Toulouse [Toulouse]-Hôpital Purpan [Toulouse], CHU Toulouse [Toulouse], Centre de Physiopathologie Toulouse Purpan (CPTP), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Medical Microbiology & Infectious Diseases, University of Padova Medical School, Génomique évolutive, modélisation et santé (CNRS-UMR2000), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Laboratoire de Parasitologie-Mycologie, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Henri Mondor-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), CHU Henri Mondor, Unité de Parasitologie-Mycologie, Service de Microbiologie [Hôpital Necker-Enfants-Malades, Paris], Assistance Publique - Hôpitaux de Paris, Centre National de Référence Mycoses Invasives et Antifongiques - National Reference Center Invasive Mycoses & Antifungals (CNRMA), Institut Pasteur [Paris]-Université Paris Cité (UPCité), Laboratoire de Parasitologie-Mycologie [CHU Saint Louis, Paris], Groupe Hospitalier Saint Louis - Lariboisière - Fernand Widal [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Westerdijk Fungal Biodiversity Institute, and Westerdijk Fungal Biodiversity Institute - Medical Mycology

Subjects
0301 basic medicine, Mucorales, Veterinary medicine, Lichtheimia corymbifera, 030106 microbiology, Pcr assay, Mucorales PCR, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, Real-Time Polymerase Chain Reaction, law.invention, Rhizomucor pusillus, Hospitals, University, 03 medical and health sciences, interlaboratory assay, 0302 clinical medicine, SDG 3 - Good Health and Well-being, law, Humans, Mucormycosis, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, 030212 general & internal medicine, DNA, Fungal, Polymerase chain reaction, Observer Variation, standardization, biology, Clinical Laboratory Techniques, Reproducibility of Results, General Medicine, circulating DNA, biology.organism_classification, University hospital, Diagnostic strategy, DNA extraction, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Infectious Diseases, Molecular Diagnostic Techniques, France, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract

Interlaboratory evaluations of Mucorales qPCR assays were developed to assess the reproducibility and performance of methods currently used. The participants comprised 12 laboratories from French university hospitals (nine of them participating in the Modimucor study) and 11 laboratories participating in the Fungal PCR Initiative. For panel 1, three sera were each spiked with DNA from three different species (Rhizomucor pusillus, Lichtheimia corymbifera, Rhizopus oryzae). For panel 2, six sera with three concentrations of R. pusillus and L. corymbifera (1, 10, and 100 genomes/ml) were prepared. Each panel included a blind negative-control serum. A form was distributed with each panel to collect results and required technical information, including DNA extraction method, sample volume used, DNA elution volume, qPCR method, qPCR template input volume, qPCR total reaction volume, qPCR platform, and qPCR reagents used. For panel 1, assessing 18 different protocols, qualitative results (positive or negative) were correct in 97% of cases (70/72). A very low interlaboratory variability in Cq values (SD = 1.89 cycles) were observed. For panel 2 assessing 26 different protocols, the detection rates were high (77–100%) for 5/6 of spiked serum. There was a significant association between the qPCR platform and performance. However, certain technical steps and optimal combinations of factors may also impact performance. The good reproducibility and performance demonstrated in this study support the use of Mucorales qPCR as part of the diagnostic strategy for mucormycosis.

Published
2021

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