128 results on '"Wattiaux, R."'
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2. Altered intracellular processing and enhanced secretion of procathepsin D in a highly-deviated rat hepatoma
- Author
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Isidoro, C., Demoz, M., DE STEFANIS, Daniela, Mainferme, F., Wattiaux, R., and Baccino, F. M.
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- 1995
3. Distribution intracellulaire de l'exonucléase acide dans le foie de rat.
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van Dyck, J. M. and Wattiaux, R.
- Subjects
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DNA , *LIVER , *CYTOCHROMES , *ENZYMES , *MITOCHONDRIA , *ENDOPLASMIC reticulum - Abstract
Acid exonuclease activity of rat liver has been determined with the help of DNA core as substrate, The intracellular distribution of the enzyme has been investigated by differential centrifugation of homogenates and compared with those of cytochrome oxidase, acid phosphatase and glucose-6-phosphatase used as reference enzymes for mitochondria, lysosomes and endoplasmic reticulum respectively. Acid exonuclease is principally associated with mitochondrial fractions and exhibits a distribution pattern similar to that of acid phosphatase. After isopyenic centrifugation in a sucrose gradient of a total mitoehondrial fraction, acid exonuclease like acid phosphatase shows a relatively flattened distribution curve with a median equilibrium density of about 1.2. The injection to the rat of Triton WR -1339, which lowers the equilibrium density of lysosomal enzymes has a similar effect on acid exonuclease. Acid exonuclease exhibits the phenomenom of structurelinked latency. it is concluded that acid exonuclease is associated with the lysosomes in rat liver. Separation between acid DNase and acid exonuelease associated with lysosomes can be performed by chromatography on hydroxyapatite of an extract of purified granules. The complementary action of the two enzymes in lysosomal digestion of DNA is illustrated, [ABSTRACT FROM AUTHOR]
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- 1968
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4. Distribution des activités ATP glucose 6-phosphotransférase, phosphoglucomutase et thiamine pyrophosphatase d'une fraction micro-somale de foie de rat, après centrifugation en gradient de saccharose.
- Author
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Thirion, Jacqueline and Wattiaux, R.
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- 1977
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5. Une étude par cryodécapage de la structure des mitochondries de foie de rat soumises à des pressions hydrostatiques croissantes.
- Author
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Coninck, De Simone Wattiaux, Dubois, F., and Wattiaux, R.
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- 1977
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6. Isolement d'une préparation purifiée de lysosomes de foie de rat par centrifligation dans un gradient de métrizamide.
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Wattiaux, R., Coninck, De Simone Wattiaux, and Dubois, F.
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- 1977
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7. Enzymic Content of the Mitochondria Fraction.
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DUVE, C. DE, GIANETTO, R., APPELMANS, F., and WATTIAUX, R.
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- 1953
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8. Subcellular distribution of adenylate cyclase in rat-liver tissue
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Wattiaux-de Coninck, S., Dubois, F., and Wattiaux, R.
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- 1981
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9. Ageing, collagen and enzymatic activity in two strains of the laboratory mouse
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Elens, A. and Wattiaux, R.
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- 1970
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10. Submitochondrial localization of DNA polymerase in rat liver tissue
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Wattiaux-De Coninck, S., Dubois, F., and Wattiaux, R.
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- 1973
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11. Subcellular particles in tumors—IV Lysosomes in hepatoma HC and Morris hepatomas 7794A, 7794B, 5123A, 7316A and 16
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Wattiaux, R., Wattiaux-De Coninck, S., Dijck, J.M.Van, Dupal, M.F., and Morris, H.P.
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- 1970
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12. Subcellular particles in tumors—V.: Behavior of mitochondria of Hepatoma HC and Morris Hepatomas 7316A, 7794B and 16 in density-gradient centrifugation
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Wattiaux-de Coninck, S., Collot, M., Wattiaux, R., and Morris, H.P.
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- 1972
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13. Subcellular particles in tumors—III: Peroxisomal enzymes in hepatoma HC and Morris hepatomas 7794A, 7794B, 5123A and 7316A
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Wattiaux, R., Wattiaux-De Coninck, S., Van Dijck, J.M., and Morris, H.P.
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- 1970
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14. Influence of the injection of ‘Triton WR-1339’ on lysosomes of a rat transplanlable hepatoma.
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Wattiaux, R. and Wattiaux-de, Coninck S.
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- 1968
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15. 8 - Centrifugation of Subcellular Components
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WATTIAUX, R.
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- 1971
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16. Age-correlated changes in lysosomal enzyme activities: An index of ageing?
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Elens, A. and Wattiaux, R.
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- 1969
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17. Stabilization of rat liver lysosomes by heparin in vitro
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Trigaux, J.P., Ronveaux-Dupal, M.F., and Wattiaux, R.
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- 1972
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18. 2.3 - DRUGS AND LYSOSOMES
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WATTIAUX, R.
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- 1971
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19. 99 Effects of fat-soluble compounds on lysosomes in vitro
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de Duve, C., Wattiaux, R., and Wibo, M.
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- 1961
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20. Endocytosis of hyaluronidase-1 by the liver.
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Gasingirwa MC, Thirion J, Mertens-Strijthagen J, Wattiaux-De Coninck S, Flamion B, Wattiaux R, and Jadot M
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- Animals, Humans, Hyaluronoglucosaminidase genetics, Male, Mice, Mice, Inbred C57BL, Mice, Knockout, Endocytosis, Hyaluronoglucosaminidase metabolism, Liver enzymology
- Abstract
It has been suggested that intracellular Hyal-1 (hyaluronidase-1), which is considered a lysosomal enzyme, originates via endocytosis of the serum enzyme. To test this proposal we have investigated the uptake and intracellular distribution of rhHyal-1 (recombinant human Hyal-1) by mouse liver, making use of centrifugation methods. Experiments were performed on wild-type mice injected with 125I-labelled rhHyal-1 and on Hyal-1-/- mice injected with the unlabelled enzyme, which were killed at various times after injection. Activity of the unlabelled enzyme was determined by zymography. Intracellular distribution of Hyal-1 was investigated by differential and isopycnic centrifugation. The results of the study indicated that rhHyal-1 is endocytosed by the liver, mainly by sinusoidal cells, and follows the intracellular pathway described for many endocytosed proteins that are eventually located in lysosomes. However, Hyal-1 endocytosis has some particular features. First, endocytosed rhHyal-1 is quickly degraded. Secondly, its distribution, as analysed by differential centrifugation, differs from the distribution of beta-galactosidase, taken as the reference lysosomal enzyme. Further analysis by isopycnic centrifugation in a sucrose gradient shows endocytosed rhHyal-1 behaves like beta-galactosidase shortly after injection. However the Hyal-1 distribution is markedly less affected than beta-galactosidase, following a prior injection of Triton WR-1339, which is a specific density perturbant of lysosomes. The behaviour in centrifugation of endogenous liver Hyal-1, identified by hyaluronan zymography, exhibits some similarity with the behaviour of the endocytosed enzyme, suggesting that it could originate from endocytosis of the serum enzyme. Overall, these results can be explained by supposing that active endocytosed Hyal-1 is mainly present in early lysosomes. Although its degradation half-time is short, Hyal-1 could exert its activity due to a constant supply of active molecules from the blood.
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- 2010
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21. Lysosomes and Fas-mediated liver cell death.
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Wattiaux R, Wattiaux-de Coninck S, Thirion J, Gasingirwa MC, and Jadot M
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- Animals, Caspase 3 metabolism, Cell Death, Cell Fractionation, Female, Hepatocytes cytology, Hepatocytes physiology, Humans, Jurkat Cells, Liver physiology, Mice, Mice, Inbred Strains, Liver cytology, Lysosomes physiology, fas Receptor physiology
- Abstract
A number of studies, mostly performed ex vivo, suggest that lysosomes are involved in apoptosis as a result of a release of their cathepsins into the cytosol. These enzymes could then contribute to the permeabilization of the outer mitochondrial membrane; they could also activate effector caspases. The present study aims at testing whether the membrane of liver lysosomes is disrupted during Fas-mediated cell death of hepatocytes in vivo, a process implicated in several liver pathologies. Apoptosis was induced by injecting mice with aFas (anti-Fas antibody). The state of lysosomes was assessed by determining the proportion of lysosomal enzymes (beta-galactosidase, beta-glucuronidase, cathepsin C and cathepsin B) present in homogenate supernatants, devoid of intact lysosomes, and by analysing the behaviour in differential and isopycnic centrifugation of beta-galactosidase. Apoptosis was monitored by measuring caspase 3 activity (DEVDase) and the release of sulfite cytochrome c reductase, an enzyme located in the mitochondrial intermembrane space. Results show that an injection of 10 microg of aFas causes a rapid and large increase in DEVDase activity and in unsedimentable sulfite cytochrome c reductase. This modifies neither the proportion of unsedimentable lysosomal enzyme in the homogenates nor the behaviour of lysosomes in centrifugation. Experiments performed with a lower dose of aFas (5 microg) indicate that unsedimentable lysosomal hydrolase activity increases in the homogenate after injection but with a marked delay with respect to the increase in DEVDase activity and in unsedimentable sulfite cytochrome c reductase. Comparative experiments ex vivo performed with Jurkat cells show an increase in unsedimentable lysosomal hydrolases, but much later than caspase 3 activation, and a release of dipeptidyl peptidase III and DEVDase into culture medium. It is proposed that the weakening of lysosomes observed after aFas treatment in vivo and ex vivo results from a necrotic process that takes place late after initiation of apoptosis.
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- 2007
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22. Hydrodynamics-based transfection of the liver: entrance into hepatocytes of DNA that causes expression takes place very early after injection.
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Andrianaivo F, Lecocq M, Wattiaux-De Coninck S, Wattiaux R, and Jadot M
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- Animals, Cell Membrane metabolism, DNA, Circular metabolism, Female, Injections, Intraperitoneal, Injections, Intravenous, Kinetics, Luciferases metabolism, Mice, Sodium Chloride administration & dosage, Time Factors, Tissue Distribution, DNA, Circular administration & dosage, Gene Expression, Hepatocytes metabolism, Liver metabolism, Plasmids genetics, Transfection methods
- Abstract
Background: The mechanism of gene transfer into hepatocytes by the hydrodynamics-based transfection procedure is not clearly understood. It has been shown that, after a hydrodynamic injection, a large proportion of plasmid DNA remains intact in the liver where it is bound to plasma membrane and suggested that this DNA could be responsible for the efficiency of the transfection., Methods: We have investigated the problem by giving mice a hydrodynamic injection of isotonic NaCl, followed at different time intervals by a conventional injection of DNA, cold or labelled with (35)S, with cDNA of luciferase as a reporter gene. Then, we determined the consequences of that dual injection on luciferase expression and on DNA uptake by the liver and its intracellular fate. By such experiments, it is possible to establish the time dependency of the induction of liver changes caused by a hydrodynamic injection on the one hand and the expression and DNA uptake and fate on the other. Moreover, some experiments have been performed on primary cultures of hepatocytes isolated after a hydrodynamic injection of DNA., Results: When DNA is given to mice by a conventional injection a few seconds after an hydrodynamic injection of isotonic NaCl, luciferase expression in the liver is considerably lower than that observed after a single hydrodynamic injection of the plasmid. On the other hand, as assessed by the rate of DNA degradation and by centrifugation results obtained after injection of (35)S-DNA, the uptake and the intracellular fate of the bulk of DNA are similar whether DNA is administered by a single hydrodynamic injection or by a conventional injection given up to at least 2 h after a hydrodynamic injection of isotonic NaCl. Hepatocytes isolated a few minutes after a hydrodynamic injection exhibit a maximal expression that does not depend on the large amount of DNA that remains bound to the plasma membrane for a relatively long time., Conclusions: Our results show that the efficiency of hydrodynamics-based transfection depends on a process that takes place very quickly after injection and is not linked to a delay of DNA degradation and the persistence of a large proportion of DNA bound to hepatocytes of the plasma membrane, strongly suggesting that expression after a hydrodynamic injection is caused by a small proportion of DNA molecules that rapidly enter the cytosol probably by plasma membrane pores generated by the hydrodynamic pressure.
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- 2004
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23. A comparative study of the subcellular distribution of native and deglycosylated gelonin in rat liver and kidney.
- Author
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Colaço M, Misquith S, Bapat MM, Wattiaux-De Coninck S, and Wattiaux R
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- Animals, Centrifugation, Glycosylation, Iodine Radioisotopes metabolism, Male, Plant Proteins chemistry, Protein Synthesis Inhibitors chemistry, Rats, Rats, Wistar, Ribosome Inactivating Proteins, Type 1, Subcellular Fractions chemistry, Kidney metabolism, Liver metabolism, Plant Proteins metabolism, Protein Synthesis Inhibitors metabolism, Subcellular Fractions metabolism
- Abstract
Intravenous injection of gelonin and deglycosylated gelonin led to rapid clearance from the blood. Both molecules distributed similarly in liver and kidney suggesting that they followed the same pathway. Deglycosylation reduced the uptake by a third in liver, but did not affect uptake by kidney. Studies with Triton WR1339 showed a classical lysosomal pathway for both molecules. The deglycosylated molecule was degraded to a greater extent than native gelonin as seen by the presence of acid soluble radioactivity. Cell separation showed that while endothelial cells mainly took up native gelonin, Kupffer cells took up the deglycosylated molecule.
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- 2004
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24. The acid phosphatase positive organelles of the Giardia lamblia trophozoite contain a membrane bound cathepsin C activity.
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Thirion J, Wattiaux R, and Jadot M
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- Animals, Biomarkers analysis, Cathepsin C analysis, Cholesterol, Cyclodextrins, Lysosomes chemistry, Lysosomes enzymology, Membrane Microdomains chemistry, Membrane Proteins analysis, Octoxynol, Organelles enzymology, Subcellular Fractions chemistry, Acid Phosphatase analysis, Cathepsin C metabolism, Giardia lamblia ultrastructure, Membrane Proteins metabolism, Organelles chemistry
- Abstract
We found a dipeptidyl aminopeptidase activity in the parasitic protozoan Giardia lamblia with properties similar to the lysosomal cathepsin C of rat-liver lysosomes. Subcellular fractionation of this parasite indicated that the cathepsin C activity is located in organelles not distinguishable from the ones containing acid phosphatase, a known marker enzyme of Giardia lysosome-like peripheral vesicles. Contrary to the rat lysosomal enzyme, Giardia cathepsin C behaved like a membrane protein. Moreover, the enzyme was not solubilized by Triton X-100 or Triton X-100/SDS at 0 degrees C but could be substantially solubilized by octylglucoside, Triton X-100 at 37 degrees C or by a pretreatment with the cholesterol complexing agent beta-cyclodextrin before the Triton/SDS treatment carried out at 0 degrees C. These observations suggest that binding/anchorage of this enzyme to membranes occurs in cholesterol-rich microdomains.
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- 2003
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25. Uptake by mouse liver and intracellular fate of plasmid DNA after a rapid tail vein injection of a small or a large volume.
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Lecocq M, Andrianaivo F, Warnier MT, Wattiaux-De Coninck S, Wattiaux R, and Jadot M
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- Animals, Cathepsin C metabolism, Cell Fractionation, Cell Membrane metabolism, DNA metabolism, Deoxyribonuclease I metabolism, Female, Glucosidases metabolism, Liver cytology, Liver physiology, Mice, Phosphodiesterase I metabolism, Plasmids metabolism, Subcellular Fractions chemistry, Subcellular Fractions metabolism, Sulfur Radioisotopes metabolism, beta-Galactosidase metabolism, DNA administration & dosage, Gene Transfer Techniques, Liver metabolism, Plasmids administration & dosage, Plasmids genetics
- Abstract
Background: An efficient gene transfer can be achieved in mouse liver by a rapid tail vein injection of a large volume of plasmid DNA solution (hydrodynamics-based transfection). The mechanism of gene transfer by this procedure is not known. It must be related to the uptake and intracellular fate of DNA., Methods: We have investigated the problem by following the uptake by mouse liver and the intracellular distribution of DNA after a rapid tail vein injection of a large (2.0 ml) or a small (0.2 ml) volume of (35)S-DNA solution. Total and acid-soluble radioactivity were measured in liver homogenates at increasing times after injection, and their subcellular distributions were established by centrifugation methods and compared with the distributions of marker enzymes of the membrane compartments involved in endocytosis: alkaline phosphodiesterase (plasma membrane) and cathepsin C (lysosomes)., Results: (35)S-DNA uptake by the liver is similar when a small or a large volume of injection is used but its degradation is markedly slower after a 2.0 ml injection. When a small volume of injection is given, distribution of radioactivity after differential centrifugation indicates that the plasmid DNA is endocytosed and reaches lysosomes where it is hydrolysed. After a large volume injection, part of (35)S-DNA has the same fate, another part remains acid-precipitable for at least 1 h and is associated with structures sedimenting at low centrifugation speed in the nuclear fraction N. Analysis of that fraction by gradient centrifugation suggests that these structures are plasma membrane fragments that could originate from the apical domain of hepatocytes. The proportion of (35)S-DNA associated with hepatocytes is about doubled after a large volume injection. Fractionation of isolated hepatocytes by centrifugation confirms results obtained on the whole liver. Treatment of the N fraction or isolated hepatocytes with pancreatic DNAse illustrates that (35)S-DNA that remains bound to plasma membrane after a large volume injection is located on the outer face., Conclusions: The fact that after an hydrodynamic injection (35)S-DNA remains bound to the outside face of the plasma membrane for at least 1 h indicates that it is not, or very slowly, internalised during that period. The relatively small difference in the amount of DNA picked up by hepatocytes depending on the type of injection could not explain the absence of expression after a conventional injection and the strong expression after a hydrodynamic injection. If DNA enters the cells by endocytosis, even after an hydrodynamic injection, its persistence at the outside face of the plasma membrane could favour transfection by allowing hepatocytes to dispose for a relatively long time of a reservoir of intact DNA., (Copyright 2002 John Wiley & Sons, Ltd.)
- Published
- 2003
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26. Uptake and intracellular fate of gelonin, a ribosome-inactivating protein, in rat liver.
- Author
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Colaço M, Bapat MM, Misquith S, Jadot M, Wattiaux-De Coninck S, and Wattiaux R
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- Animals, Cell Extracts chemistry, Centrifugation, Isopycnic, Dipeptides pharmacology, Electrophoresis, Polyacrylamide Gel, Glycoside Hydrolases pharmacology, Kinetics, Lysosomes drug effects, Male, Microsomes, Liver chemistry, Mitochondria, Liver chemistry, Mitochondria, Liver drug effects, Plant Proteins analysis, Protein Synthesis Inhibitors analysis, Protein Transport, Rats, Rats, Wistar, Ribosome Inactivating Proteins, Type 1, Ribosomes drug effects, beta-Fructofuranosidase, Liver metabolism, Plant Proteins metabolism, Protein Synthesis Inhibitors metabolism
- Abstract
Gelonin, a type 1 ribosome-inactivating protein, has been used as toxin conjugate for several therapeutic purposes. We have investigated the endocytosis of gelonin by rat liver in vivo. Subcellular distribution of [125I]gelonin was established after differential and isopycnic centrifugation. Fractions were analyzed for acid-soluble and acid-precipitable radioactivity. Results show that gelonin is rapidly cleared from the blood and within 15min reaches a peak (25% of total injected) in the liver. With time, radioactivity associated with the liver markedly decreases. Two important observations are made: (a) Radioactivity associated with all fractions, at any time point, is greater than 80% acid precipitable. (b) Even at 5min, a significant amount of intact gelonin is present in the cytosolic fraction. Our work suggests that, though gelonin is rapidly cleared from the blood, there are still intact molecules that have entered the cytosol where they could exert their toxic effect.
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- 2002
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27. Effects of methylcyclodextrin on lysosomes.
- Author
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Jadot M, Andrianaivo F, Dubois F, and Wattiaux R
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- Cathepsin C metabolism, Centrifugation, Isopycnic, Dipeptides pharmacology, Glucose metabolism, Humans, Hydrostatic Pressure, Hypotonic Solutions, Isotonic Solutions, Lysosomes enzymology, Osmotic Pressure, Permeability, Phosphodiesterase I, Phosphoric Diester Hydrolases metabolism, Tumor Cells, Cultured, beta-Galactosidase metabolism, Cyclodextrins pharmacology, Lysosomes drug effects, beta-Cyclodextrins
- Abstract
The cholesterol complexing agent methyl-cyclodextrin (MCD) provides an efficient mean for the removal of cholesterol from biological membranes. In order to study the effects of this agent on the lysosomal membrane in situ, we treated HepG2 cells with MCD and studied the effects of this treatment on lysosomes in isolated fractions. We found that lysosomes prepared from treated cells are more sensitive to various membrane perturbing treatments such as: incubation of lysosomes in isotonic glucose, in hypotonic sucrose or in the presence of the lytic agent glycyl-L-phenylalanine 2-naphthylamide. The lysosomal membrane is also less resistant to increased hydrostatic pressure. Centrifugation methods were used to analyse the effect of MCD on lysosomes. Isopycnic centrifugation in sucrose density gradients demonstrates that the drug induces a reversible density increase of the lysosomes. Our study indicates that extracellularly added MCD can modify the properties of the lysosomal membrane in living cells. It suggests that MCD could be an effective tool to modulate the physical properties of lysosomes within intact cells and to monitor the cellular responses to such modifications.
- Published
- 2001
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28. Identification of HE1 as the second gene of Niemann-Pick C disease.
- Author
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Naureckiene S, Sleat DE, Lackland H, Fensom A, Vanier MT, Wattiaux R, Jadot M, and Lobel P
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- Amino Acid Sequence, Animals, Biological Transport, CHO Cells, Cell Membrane metabolism, Cells, Cultured, Cricetinae, Culture Media, Conditioned, Fibroblasts metabolism, Glycoproteins chemistry, Glycoproteins pharmacology, Humans, Molecular Sequence Data, Mutation, Niemann-Pick Diseases metabolism, Rats, Receptor, IGF Type 2 metabolism, Recombinant Proteins metabolism, Recombinant Proteins pharmacology, Transfection, Vesicular Transport Proteins, Carrier Proteins, Cholesterol metabolism, Glycoproteins genetics, Glycoproteins metabolism, Lysosomes metabolism, Niemann-Pick Diseases genetics
- Abstract
Niemann-Pick type C2 disease (NP-C2) is a fatal hereditary disorder of unknown etiology characterized by defective egress of cholesterol from lysosomes. Here we show that the disease is caused by a deficiency in HE1, a ubiquitously expressed lysosomal protein identified previously as a cholesterol-binding protein. HE1 was undetectable in fibroblasts from NP-C2 patients but present in fibroblasts from unaffected controls and NP-C1 patients. Mutations in the HE1 gene, which maps to chromosome 14q24.3, were found in NP-C2 patients but not in controls. Treatment of NP-C2 fibroblasts with exogenous recombinant HE1 protein ameliorated lysosomal accumulation of low density lipoprotein-derived cholesterol.
- Published
- 2000
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29. Uptake and intracellular fate of polyethylenimine in vivo.
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Lecocq M, Wattiaux-De Coninck S, Laurent N, Wattiaux R, and Jadot M
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- Animals, Iodine Radioisotopes metabolism, Liver metabolism, Male, Rats, Polyethyleneimine metabolism
- Abstract
Branched polyamines are extensively used as nonviral vectors for plasmid DNA in transfection experiments. Moreover, recently it has been shown that these compounds are able to eliminate prions from infected cells in cultures. It has been proposed that in both cases endosomes or lysosomes are the site of action. This raises the question of how these molecules are taken up by the cells and what is their intracellular fate. In the work presented here, the question has been addressed by investigating the uptake and the intracellular distribution of branched polyethyleneimine (25 kD) by centrifugation methods. The polyamine was labelled with (125)I-tyramine cellobiose and injected to the rat. The radioactive polymer is taken up after injection into the liver, kidney, spleen, and lungs and remains in these organs for many days. In the liver, it is found mainly in the hepatocytes. Intracellular distribution of radioactivity present in that organ was investigated by differential and isopycnic centrifugations. Early after injection, radioactivity exhibits a distribution pattern similar to that of alkaline phosphodiesterase, a plasma membrane marker. Later, the distribution pattern becomes similar to that of cathepsin C, a lysosomal enzyme. Radioactivity and hydrolase distributions in a sucrose gradient are similarly modified by a pretreatment of the rat with Triton-WR1339, a specific density perturbant of lysosomes. These results indicate that polyethyleneimine is endocytosed and reaches lysosomes. For many days it persists in these organelles probably due to its resistance to lysosomal hydrolases., (Copyright 2000 Academic Press.)
- Published
- 2000
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30. Endosomes, lysosomes: their implication in gene transfer.
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Wattiaux R, Laurent N, Wattiaux-De Coninck S, and Jadot M
- Subjects
- Animals, DNA metabolism, Endocytosis, Genetic Vectors, Humans, Endosomes metabolism, Gene Transfer Techniques, Lysosomes metabolism
- Abstract
Plasmid DNA, naked or bound to a non-viral vector, is taken up by endocytosis. As a result, it has to travel through the intracellular endocytic pathway involving endosomes and lysosomes. However, some DNA molecules must escape these organelles to reach the nucleus where transcription takes place. In this paper, we consider different factors that could affect the trafficking of plasmid DNA and influence transfection efficiency.
- Published
- 2000
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31. Subcellular localization of mannose 6-phosphate glycoproteins in rat brain.
- Author
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Jadot M, Lin L, Sleat DE, Sohar I, Hsu MS, Pintar J, Dubois F, Wattiaux-De Coninck S, Wattiaux R, and Lobel P
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- Animals, Biological Transport, Lysosomes metabolism, Male, Neurons metabolism, Neurons ultrastructure, Rats, Rats, Wistar, Brain metabolism, Mannosephosphates metabolism
- Abstract
The intracellular transport of soluble lysosomal enzymes relies on the post-translational modification of N-linked oligosaccharides to generate mannose 6-phosphate (Man 6-P) residues. In most cell types the Man 6-P signal is rapidly removed after targeting of the precursor proteins from the Golgi to lysosomes via interactions with Man 6-phosphate receptors. However, in brain, the steady state proportion of lysosomal enzymes containing Man 6-P is considerably higher than in other tissues. As a first step toward understanding the mechanism and biological significance of this observation, we analyzed the subcellular localization of the rat brain Man 6-P glycoproteins by combining biochemical and morphological approaches. The brain Man 6-P glycoproteins are predominantly localized in neuronal lysosomes with no evidence for a steady state localization in nonlysosomal or prelysosomal compartments. This contrasts with the clear endosome-like localization of the low steady state proportion of mannose-6-phosphorylated lysosomal enzymes in liver. It therefore seems likely that the observed high percentage of phosphorylated species in brain is a consequence of the accumulation of lysosomal enzymes in a neuronal lysosome that does not fully dephosphorylate the Man 6-P moieties.
- Published
- 1999
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32. Uptake by rat liver and intracellular fate of plasmid DNA complexed with poly-L-lysine or poly-D-lysine.
- Author
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Laurent N, Wattiaux-De Coninck S, Mihaylova E, Leontieva E, Warnier-Pirotte MT, Wattiaux R, and Jadot M
- Subjects
- Animals, Biological Transport drug effects, Cations metabolism, Hydrolysis, Lysosomes metabolism, Male, Polyethylene Glycols pharmacology, Rats, Rats, Wistar, Stereoisomerism, Subcellular Fractions metabolism, Transfection, Genetic Vectors metabolism, Liver metabolism, Plasmids metabolism, Polylysine metabolism
- Abstract
Efficiency of transfection is probably dependent on the rate of intracellular degradation of plasmid DNA. When a non-viral vector is used, it is not known to what extent the plasmid DNA catabolism is subordinated to the catabolism of the vector. In the work reported here, the problem was approached by following the intracellular fate in rat liver, of plasmid [35S]DNA complexed with a cationic peptide poly-L-lysine that can be hydrolyzed by cellular peptidases or with its stereoisomer, poly-D-lysine, that cannot be split by these enzymes. Complexes of DNA with poly-L-lysine and poly-D-lysine are taken up to the same extent by the liver, mainly by Kupffer cells, but the intracellular degradation of nucleic acid molecules is markedly quicker when poly-L-lysine is injected. The association of DNA with the polycations inhibits DNA hydrolysis in vitro by purified lysosomes but similarly for poly-L-lysine and poly-D-lysine. The intracellular journey followed by [35S]DNA complexed with poly-L- or poly-D-lysine was investigated using differential and isopycnic centrifugation. Results indicate that [35S]DNA is transferred more slowly to lysosomes, the main site of intracellular degradation of endocytosed macromolecules, when it is given as a complex with poly-D-lysine than with poly-L-lysine. They suggest that the digestion of the vector in a prelysosomal compartment is required to allow endocytosed plasmid DNA to rapidly reach lysosomes. Such a phenomenon could explain why injected plasmid DNA is more stable in vivo when it is associated with poly-D-lysine.
- Published
- 1999
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33. Delta F508 CFTR localizes in the endoplasmic reticulum-Golgi intermediate compartment in cystic fibrosis cells.
- Author
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Gilbert A, Jadot M, Leontieva E, Wattiaux-De Coninck S, and Wattiaux R
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- Adenocarcinoma, Calcium-Binding Proteins analysis, Calnexin, Cell Fractionation, Humans, Membrane Proteins analysis, Organelles enzymology, Pancreatic Neoplasms, Tumor Cells, Cultured, Cystic Fibrosis metabolism, Cystic Fibrosis Transmembrane Conductance Regulator analysis, Endoplasmic Reticulum chemistry, Golgi Apparatus chemistry, Mannose-Binding Lectins
- Abstract
We have studied the localization of mutant cystic fibrosis transmembrane regulator delta F508CFTR in pancreatic adenocarcinoma cells (CFPAC), which naturally express the mutant protein. Our goal was to investigate whether delta F508CFTR is strictly retained in the endoplasmic reticulum (ER) or alternatively whether it can be transported beyond the ER and reach the endoplasmic reticulum-Golgi intermediate compartment (ERGIC). This compartment, defined by the presence of the 53-kDa protein ERGIC-53, was identified by subcellular fractionation and by immunofluorescence. Part of the delta F508CFTR population and ERGIC-53 showed similar distributions in membrane fractions analyzed on Nycodenz density gradients. Both proteins were present in density fractions distinct from the ones containing the ER marker proteins calnexin and Sec61. Immunofluorescence microscopy of CFPAC cells revealed some colocalization of delta F508CFTR with ERGIC-53. Following incubation of CFPAC cells at 15 degrees C, a condition known to block ER to Golgi transport, both ERGIC-53 and delta F508CFTR subcellular localizations were altered. By contrast, this temperature shift had no effect on the localization of the ER marker Sec61. Our observations indicate that the abnormal protein delta F508CFTR can leak out of the ER and reach the ERGIC. These results support the idea that this intermediate compartment plays a role in the trafficking events leading to retention and finally degradation of the misfolded delta F508CFTR protein.
- Published
- 1998
- Full Text
- View/download PDF
34. Expression of Lamp-1 and Lamp-2 and their interactions with galectin-3 in human tumor cells.
- Author
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Sarafian V, Jadot M, Foidart JM, Letesson JJ, Van den Brûle F, Castronovo V, Wattiaux R, and Coninck SW
- Subjects
- Adenocarcinoma metabolism, Antigens, CD drug effects, Antigens, Differentiation drug effects, Choriocarcinoma metabolism, Colonic Neoplasms metabolism, Fibrosarcoma metabolism, Flow Cytometry, Fluorescent Antibody Technique, Indirect, Galectin 3, Humans, Lysosomal Membrane Proteins, Melanoma metabolism, Membrane Glycoproteins drug effects, Neoplasm Proteins drug effects, Tumor Cells, Cultured drug effects, Tumor Cells, Cultured metabolism, Antigens, CD metabolism, Antigens, Differentiation metabolism, Membrane Glycoproteins metabolism, Neoplasm Proteins metabolism
- Abstract
Lysosomal-membrane-associated glycoproteins (Lamps) 1 and 2 are rarely found on the plasma membranes of normal cells. There is evidence suggesting an increase in their cell-surface expression in tumor cells, with some data indicating that the adhesion of some cancer cells to the extracellular matrix is partly mediated by interactions between Lamps and E-selectin and between Lamps and galectins (endogenous-galactoside-binding lectins). The present study examined the expression of Lamp-1 and Lamp-2 and their interactions with galectin-3 in different human tumor cell lines. Indirect immunofluorescence staining revealed accumulation of Lamp molecules at the edges of A2058 human metastasizing melanoma cells suggesting that these glycoproteins could participate in cell adhesion. Flow cytometry showed the presence of cell-surface Lamps in A2058, HT1080 (human fibrosarcoma) and CaCo-2 (human colon-adenocarcinoma) cells. Treatment with 2 mM sodium butyrate for 24 and 48 hr resulted in a significant increase in Lamps surface expression. A strong binding of A2058 to recombinant galectin-3 was detected by FACS. The application of 2 and 5 mM butyrate for the same incubation period enhanced galectin-3 binding to Lamps-expressing cells. Our results support the idea that Lamps may be considered a new family of adhesive glycoproteins participating in the complex process of tumor invasion and metastasis.
- Published
- 1998
- Full Text
- View/download PDF
35. Cationic lipids destabilize lysosomal membrane in vitro.
- Author
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Wattiaux R, Jadot M, Warnier-Pirotte MT, and Wattiaux-De Coninck S
- Subjects
- Animals, Cations, Cell-Free System, DNA chemistry, Hydrogen-Ion Concentration, Male, Plasmids, Rats, Rats, Wistar, Transfection methods, beta-Galactosidase metabolism, Fatty Acids, Monounsaturated chemistry, Intracellular Membranes chemistry, Lipids chemistry, Lysosomes chemistry, Quaternary Ammonium Compounds chemistry
- Abstract
Addition of cationic lipids to plasmid DNA considerably increases the efficiency of transfection. The mechanism has not yet been elucidated. A possibility is that these compounds destabilize biological membranes (plasma, endosomal, lysosomal), facilitating the transfer of nucleic molecules through these membranes. We have investigated the problem by determining if a cationic lipid N-(1-(2,3-dioleoxy)propyl)-N,N,N,-trimethylammonium methyl-sulfate (DOTAP, Boehringer, Mannheim, Germany) affects the integrity of rat liver lysosomal membrane. We have measured the latency of beta-galactosidase, a lysosomal enzyme, and found that incubation of lysosomes with low concentrations of DOTAP causes a striking increase in free activity of the hydrolase and even a release of the enzyme into the medium. This indicates that lysosomal membrane is deeply destabilized by the lipid. The phenomenon depends on pH, it is less pronounced at pH 5 than at pH 7.4. Anionic compounds, particularly anionic amphipathic lipids, can to some extent prevent this phenomenon. It can be observed with various cationic lipids. A possible explanation is that cationic liposomes interact with anionic lipids of lysosomal membrane, allowing a fusion between the lipid bilayers which results in a destabilization of the organelle membrane.
- Published
- 1997
- Full Text
- View/download PDF
36. Supramolecular assemblies from lysosomal matrix proteins and complex lipids.
- Author
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Jadot M, Dubois F, Wattiaux-De Coninck S, and Wattiaux R
- Subjects
- Animals, Antigens, CD chemistry, Biomarkers analysis, Centrifugation, Density Gradient, Chromatography, Gel, Electrophoresis, Polyacrylamide Gel, Endopeptidase K metabolism, Hydrogen-Ion Concentration, Lipids chemistry, Liver chemistry, Liver enzymology, Lysosomal Membrane Proteins, Lysosomes enzymology, Male, Mannosidases chemistry, Membrane Glycoproteins chemistry, Phospholipids chemistry, Phospholipids metabolism, Protein Conformation, Rats, Rats, Wistar, Sphingomyelins metabolism, alpha-Mannosidase, Antigens, CD metabolism, Lipid Metabolism, Lysosomes chemistry, Mannosidases metabolism, Membrane Glycoproteins metabolism
- Abstract
Most lysosomal hydrolases are soluble enzymes. Lamp-II (lysosome-associated membrane protein-II) is a major constituent of the lysosomal membrane. We studied the aggregation of a series of lysosomal molecules. The aggregation-sensitive lysosomal marker enzymes were optimally aggregated at intralysosomal pH. A similar pH dependence was recorded for aggregation of Lamp-II. The pH-dependent loss of solubility of isolated Lamp-II required components of the lysosome extract. Conditions of mild acid pH promoting aggregation triggered the formation of complexes with lipids of lysosomal origin. We fractionated a membrane-free lysosome extract by gel-filtration chromatography and could reconstitute assemblies in vitro from separated fractions. We found some selectivity in the lysosomal proteins binding to complex lipids, phosphatidylcholine, sphingomyelin, and phosphatidylethanolamine being most effective. We propose that the formation at pH 5.0 of such supramolecular assemblies between lysosomal proteins and lipids occurs within the intralysosomal environment. Some possible consequences of such an intralysosomal matrix formation on organelle function are discussed.
- Published
- 1997
- Full Text
- View/download PDF
37. Cationic lipids delay the transfer of plasmid DNA to lysosomes.
- Author
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Wattiaux R, Jadot M, Laurent N, Dubois F, and Wattiaux-De Coninck S
- Subjects
- Animals, Arylsulfatases analysis, Biomarkers, Cell Fractionation, Detergents, Drug Carriers, Fatty Acids, Monounsaturated, Male, Organelles metabolism, Plasmids pharmacokinetics, Polyethylene Glycols, Quaternary Ammonium Compounds, Rats, Rats, Wistar, Sulfur Radioisotopes, Liposomes, Liver metabolism, Lysosomes metabolism, Plasmids administration & dosage
- Abstract
Plasmid 35S DNA, naked or associated with different cationic lipid preparations was injected to rats. Subcellular distribution of radioactivity in the liver one hour after injection, was established by centrifugation methods. Results show that at that time, 35S DNA has reached lysosomes. On the contrary, when 35S DNA was complexed with lipids, radioactivity remains located in organelles whose distribution after differential and isopycnic centrifugation, is clearly distinct from that of arylsulfatase, lysosome marker enzyme. Injection of Triton WR 1339, a specific density perturbant of lysosomes, four days before 35S DNA injection causes a density decrease of radioactivity bearing structures, apparent one hour after naked 35S DNA injection but visible only after more than five hours, when 35S DNA associated with a cationic lipid is injected. These observations show that cationic lipids delay the transfer to lysosomes, of plasmid DNA taken up by the liver.
- Published
- 1996
- Full Text
- View/download PDF
38. Soluble form of Lamp II in purified rat liver lysosomes.
- Author
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Jadot M, Wattiaux R, Mainferme F, Dubois F, Claessens A, and Wattiaux-De Coninck S
- Subjects
- Animals, Antibodies, Monoclonal, Blotting, Western, Cell Fractionation, Hydrogen-Ion Concentration, Immunoenzyme Techniques, Lysosomal Membrane Proteins, Lysosomes ultrastructure, Male, Microscopy, Immunoelectron, Rats, Rats, Wistar, Antigens, CD analysis, Liver metabolism, Lysosomes metabolism, Membrane Glycoproteins analysis
- Abstract
Lamp II (for lysosomal associated membrane protein II) is an integral type I glycoprotein. It consists of a very large and heavily glycosylated luminal domain, a single transmembrane segment, and a short cytoplasmic tail. We show that in highly purified lysosomes from rat liver, Lamp II, immunodetected with a monoclonal antibody on Western blots, it surprisingly distributed between a membrane bound form and a "soluble" form. The partition of the protein between the membrane and the content of lysosomes is strongly pH dependent. The soluble Lamp II population is sensitive to pH dependent aggregation as it is for many lysosomal content enzymes.
- Published
- 1996
- Full Text
- View/download PDF
39. Phagocytosis by rat liver: relationships between phagosomes and lysosomes.
- Author
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Wattiaux R, Jadot M, Dubois F, and Wattiaux-De Coninck S
- Subjects
- Animals, Arylsulfatases analysis, Biomarkers, Cell Fractionation methods, Cellobiose, Centrifugation, Density Gradient, Iodine Radioisotopes, Kinetics, Lysosomes ultrastructure, Male, Phagosomes ultrastructure, Radioisotope Dilution Technique, Rats, Rats, Wistar, Time Factors, Tyramine, Liver physiology, Lysosomes physiology, Phagocytosis, Phagosomes physiology, Staphylococcus aureus
- Abstract
To study the transfer of phagocytosed components from phagosomes to lysosomes, we have investigated phagocytosis by rat liver of killed Staphylococcus aureus labelled with (125)I tyramine cellobiose. Lysosomes were identified by injecting the animals with Triton WR1339, a non ionic detergent that is endocytosed by the liver and accumulates in lysosomes, causing a marked decrease of their density; that allows these organelles to be well separated from other particles in a density gradient. Bacteria were quickly taken up by the liver; their uptake is followed by a slow degradation as ascertained by the increase of acid-soluble radioactivity in the homogenates with time. Triton WR1339 injection does not affect the uptake and the degradation of the particles. Differential centrifugation of homogenates shows that at any time after injection, most of the radioactivity is recovered in the mitochondrial fractions. Distributions of acid precipitable and acid soluble radioactivities amongst subcellular structures present in mitochondrial fractions were studied by isopycnic centrifugation in sucrose gradients, at increasing times after bacteria injection. Results show that: 1) acid-precipitable radioactivity is quasi-exclusively present in gradient fractions of high density, well separated from the fractions where there are recovered lysosomes; 2) with time, acid-soluble radioactivity is more and more associated with lysosomes, however, a significant proportion can be detected for many hours after injection, in gradient fractions where acid-precipitable radioactivity is located. The most plausible explanation of our observations is that phagocytosed particles are degraded in phagosomes and that the degradation products are delivered to lysosomes, probably by a vesicular process.
- Published
- 1996
- Full Text
- View/download PDF
40. Lysosome pharmacology and toxicology.
- Author
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Wattiaux R and Wattiaux-De Coninck S
- Subjects
- Animals, Biotransformation, Humans, Lysosomes drug effects, Pharmacokinetics, Lysosomes metabolism
- Published
- 1996
- Full Text
- View/download PDF
41. Deleterious effects of xanthine oxidase on rat liver endothelial cells after ischemia/reperfusion.
- Author
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Hamer I, Wattiaux R, and Wattiaux-De Coninck S
- Subjects
- Animals, Catalase analysis, Cell Separation, Glutathione Peroxidase analysis, In Vitro Techniques, Liver blood supply, Liver enzymology, Male, Rats, Reactive Oxygen Species adverse effects, Superoxide Dismutase analysis, Endothelium, Vascular physiopathology, Liver physiopathology, Oxidative Stress physiology, Reperfusion Injury physiopathology, Xanthine Oxidase metabolism
- Abstract
Previous studies have demonstrated that reactive oxygen species are involved in ischemic injury. The present work was undertaken to determine in vivo the role of xanthine oxidase in the oxygen free radical production during rat liver ischemia and to examine the activity of antioxidant enzymes (superoxide dismutase, catalase and glutathione peroxidase) during the same period. Our results indicate a 4-fold increase in xanthine oxidase activity between 2 and 3 hours of normothermic ischemia, in parallel with a decrease in cell viability. Moderate hypothermia delays both events. Under the same conditions, the activity of oxygen radical scavenging enzymes remains unchanged. Moreover, we have compared in vitro the susceptibility of isolated liver cells to an oxidative stress induced by O2.-, H2O2 and .OH. Our results reveal that endothelial cells are much more susceptible to reactive oxygen species than hepatocytes, probably because they lack H2O2-detoxifying enzymes. These findings suggest that xanthine oxidase might play a major role in the ischemic injury mainly at the level of the sinusoidal space where most endothelial cells are located.
- Published
- 1995
- Full Text
- View/download PDF
42. Uptake of exogenous DNA by rat liver: effect of cationic lipids.
- Author
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Wattiaux R, Jadot M, Dubois F, Misquith S, and Wattiaux-De Coninck S
- Subjects
- Animals, Biological Transport, Cell Fractionation, Centrifugation, Density Gradient, Endocytosis, Fluorescent Dyes, Kinetics, Lysosomes metabolism, Male, Mitochondria, Liver metabolism, Organelles metabolism, Radioisotope Dilution Technique, Rats, Rats, Wistar, Sulfur Radioisotopes, DNA metabolism, Fatty Acids, Monounsaturated pharmacology, Liver metabolism, Quaternary Ammonium Compounds pharmacology
- Abstract
We have investigated by using centrifugation methods, the uptake and the intracellular fate of 35S DNA by rat liver and the effect on these processes of N-(1-(2,3-dioleoxyloxy)propyl)-N,N,N,-trimethylammonium-methyl-sul fate(DOTAP, Boehringer, Mannheim, Germany), an artificial cationic lipid frequently used in transfection experiments. Labeled DNA molecules are quickly taken up by the liver but a progressive degradation takes place with time. Subcellular distribution of the radioactivity was established after differential and isopycnic centrifugation. Results indicate that 35S DNA enters liver cells by endocytosis and reaches lysosomes. The uptake of 35S DNA is not modified if the molecule is associated with DOTAP but marked differences are observed after internalization of the macromolecule. When DOTAP is used, radioactive products remain for a long time in low density organelles distinct from lysosomes indicating that the transfer of internalized DNA to these organelles is delayed by the cationic lipid. These results suggest that cationic lipids could favor transfection by preventing the delivery of DNA to lysosomes, allowing these molecules to be kept intact and available for transfer from endosomes to cytosol for a long time.
- Published
- 1995
- Full Text
- View/download PDF
43. Altered intracellular processing and enhanced secretion of procathepsin D in a highly deviated rat hepatoma.
- Author
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Isidoro C, Demoz M, De Stefanis D, Mainferme F, Wattiaux R, and Baccino FM
- Subjects
- Animals, Cathepsin D biosynthesis, Enzyme Precursors biosynthesis, Hydrogen-Ion Concentration, Intracellular Fluid metabolism, Liver metabolism, Liver ultrastructure, Liver Neoplasms, Experimental ultrastructure, Rats, Tumor Cells, Cultured, Vacuoles metabolism, Cathepsin D metabolism, Enzyme Precursors metabolism, Liver Neoplasms, Experimental metabolism
- Abstract
Both freshly-isolated rat hepatocytes and Morris hepatoma 7777 cells synthesized cathepsin D as a precursor that was either processed intracellular to smaller mature forms or secreted into the medium. The pattern of mature enzyme forms was different in the 2 cell types. In addition, the relative amount of precursor secreted was much higher for hepatoma cells. Monensin strongly enhanced the secretion and also impaired the intracellular transport-linked maturation of procathepsin D in hepatocytes, while it markedly inhibited intracellular maturation and only slightly increased secretion of the pro-enzyme in hepatoma cells. Ammonium chloride influenced the intralysosomal segregation and maturation of procathepsin D in hepatocytes but not in hepatoma cells. Our observations indicate that (i) the lysosomal segregation of cathepsin D was less efficient and its fractional secretion higher in hepatoma cells than in hepatocytes; (ii) in the 2 cell types, delivery to lysosomes and processing of procathepsin D were differently sensitive to increases in the vacuolar pH.
- Published
- 1995
- Full Text
- View/download PDF
44. Uptake of dopamine by rat hepatocytes in vitro.
- Author
-
Zhong ZD, Wattiaux-de Coninck S, and Wattiaux R
- Subjects
- Animals, Biological Transport drug effects, Catecholamines pharmacology, Cells, Cultured, Hydrogen-Ion Concentration, Intercellular Signaling Peptides and Proteins, Liver cytology, Male, Peptides, Rats, Rats, Wistar, Temperature, Tyramine pharmacology, Wasp Venoms pharmacology, Dopamine metabolism, Liver metabolism
- Abstract
The present results showed that uptake of dopamine (DA) by rat isolated hepatocytes was mediated, in addition to simple diffusion, mainly by a transporter-involved process, with Km of 66.8 mumol and Vmax of 52.3 pmol.min-1/10(5) cells. The process was pH- and temperature-dependent and required an activation energy of 4.12 kcal.mol-1 (Q10 = 1.25) in the range of 2.0-12.7 C and 13.0 kcal.mol-1 (Q10 = 2.0) in the range of 12.7-39.0 C. Cysteine residue having free thiol group was unrelated to the activity of the transporter. Catecholamines, serotonin, and cocaine inhibited the DA transport, but tyramine (TA) and tryptamine, as well as benztropine and imipramine (which are potent inhibitors for hepatic TA transporter and neuronal DA transporter), had no inhibitory effect on the transport of DA in these cells. These results indicated that DA was taken up into hepatocytes by a distinct carrier. NaF and mastoparan influenced the transport activity in these cells further, suggesting that signal transducing G-proteins may be involved in the regulation of DA transporter in rat hepatocytes.
- Published
- 1994
45. Ischemic effects on the structure and function of the plasma membrane.
- Author
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Wattiaux-De Coninck S and Wattiaux R
- Subjects
- Animals, Humans, Ischemia metabolism, Cell Membrane physiology, Ischemia physiopathology
- Published
- 1994
- Full Text
- View/download PDF
46. Uptake by rat liver of bovine growth hormone free or bound to a monoclonal antibody.
- Author
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Tans C, Dubois F, Zhong ZD, Jadot M, Wattiaux R, and Wattiaux-De Coninck S
- Subjects
- Animals, Cattle, Growth Hormone blood, Iodine Isotopes, Lysosomes metabolism, Male, Protein Binding, Rats, Rats, Wistar, Antibodies, Monoclonal metabolism, Growth Hormone pharmacokinetics, Liver metabolism
- Abstract
In the work reported here, we have compared the elimination from the blood, the uptake by the liver and the intracellular distribution of bovine growth hormone, free(Gh) or bound to a monoclonal antibody (GhAb). Results show that: a) the elimination from the blood is more rapid for Gh than for GhAb; b) both molecules are quickly taken up by the liver; c) probably after travelling through endosomes, Gh and GhAb get to lysosomes where they are degraded. However, Gh mostly ends in hepatocyte lysosomes while GhAb is recovered to a large extent in sinusoidal cell lysosomes; and d) binding by isolated hepatocytes is markedly less efficient for GhAb than for Gh.
- Published
- 1994
- Full Text
- View/download PDF
47. Uptake of tyramine by rat hepatocytes.
- Author
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Zhong ZD, Wattiaux-De Coninck S, and Wattiaux R
- Subjects
- Animals, Azides pharmacology, Biological Transport, Active drug effects, Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone pharmacology, Cyanides pharmacology, Diffusion, Hydrogen-Ion Concentration, Kinetics, Liver drug effects, Male, Membrane Potentials drug effects, Oligomycins pharmacology, Rats, Rats, Wistar, Structure-Activity Relationship, Temperature, Liver metabolism, Tyramine metabolism
- Abstract
Observations on the uptake of tyramine by hepatocytes indicate that the amine is taken up by simple diffusion and a transporter mediated system, with a Km of 39 microM and a Vmax of 270 pmol/min/10(5) cells. The carrier-mediated process is pH- and temperature-dependent and requires an activation energy of 12.9 kcal/mol. An overshoot uptake is achieved a few minutes after adding this amine to the cell suspension, suggesting that active transport is involved. This is supported by the finding that partial inhibition of the uptake can be induced by oligomycin, azide, cyanide and dinitrophenol. NO3-, SCN- and SO4(2-), which change the membrane potential significantly, and depress the transporter mediated uptake further, suggesting that the membrane potential is the driving force for the entry of this amine across hepatic membrane. Cysteine is essential for the normal carrier function; whereas, histidine, tryptophan, arginine and lysine do not directly deal with the activity of the carrier. Many substances, but not amino acids, H, M, and N receptor agonists, can inhibit the uptake of tyramine. It is possible that other amines can enter hepatocytes by using this transporter.
- Published
- 1993
- Full Text
- View/download PDF
48. Chloroquine allows to distinguish between hepatocyte lysosomes and sinusoidal cell lysosomes.
- Author
-
Wattiaux R, Gentinne F, Jadot M, Dubois F, and Wattiaux-De Coninck S
- Subjects
- Animals, Cell Fractionation methods, Centrifugation, Density Gradient, Male, Rats, Rats, Wistar, Subcellular Fractions, Chloroquine pharmacology, Liver ultrastructure, Lysosomes drug effects
- Abstract
We have examined the effect of chloroquine on rat liver lysosomal enzyme distributions after isopycnic centrifugation in a sucrose gradient. Chloroquine injection causes the large majority of cathepsin C, acid phosphatase and N acetyl glucosaminidase to migrate towards lower density regions; on the other hand only about 50% of arylsulfatase and acid deoxyribonuclease are subjected to such a density shift. To specifically mark hepatocyte lysosomes and sinusoidal cell lysosomes, rats were injected with galactosylated bovine serum albumin (A) or mannosylated bovine serum albumin (B) labelled with 125I tyramine cellobiose; A is selectively endocytosed by hepatocytes, B by sinusoidal cells. The radioactivity distribution is affected by chloroquine in the same way as cathepsin C, after injection of A though it is not influenced by chloroquine after the injection of B. These results show that chloroquine does not modify the density of liver sinusoidal cell lysosomes when it decreases the density of hepatocyte lysosomes. Such a difference could result from the fact that sinusoidal cell lysosomes do not accumulate chloroquine to the same extent as hepatocyte lysosomes. Chloroquine treatment could be useful to distinguish between hepatocyte lysosomes and sinusoidal cell lysosomes.
- Published
- 1993
- Full Text
- View/download PDF
49. Characterization of endocytic components of liver nonparenchymal cells.
- Author
-
Wattiaux R, Jadot M, Misquith S, and Wattiaux-de Coninck S
- Subjects
- Animals, Biological Transport, Cell Fractionation methods, Centrifugation, Density Gradient methods, Liver cytology, Liver ultrastructure, Organelles ultrastructure, Rats, Receptors, Cell Surface metabolism, Receptors, Cell Surface physiology, Serum Albumin, Bovine metabolism, Endocytosis, Liver metabolism, Organelles metabolism
- Published
- 1993
- Full Text
- View/download PDF
50. Effect of various flavonoids on lysosomes subjected to an oxidative or an osmotic stress.
- Author
-
Decharneux T, Dubois F, Beauloye C, Wattiaux-De Coninck S, and Wattiaux R
- Subjects
- Acetylglucosaminidase metabolism, Animals, Catechin pharmacology, Glucose metabolism, Intracellular Membranes drug effects, Lysosomes chemistry, Lysosomes ultrastructure, Male, Malondialdehyde analysis, Microsomes, Liver drug effects, Osmosis, Oxidation-Reduction, Quercetin analogs & derivatives, Quercetin pharmacology, Rats, Rats, Wistar, Xanthine Oxidase metabolism, Flavonoids pharmacology, Kaempferols, Lysosomes drug effects
- Abstract
When a light mitochondrial fraction (L fraction) of rat liver is incubated in the presence of an oxygen free radical generating system (xanthine-xanthine oxidase), the free activity of N-acetylglucosaminidase (NAGase) increases as a result of the deterioration of the lysosomal membrane. Various flavonoids are able to prevent this phenomenon, others are ineffective. Comparative activity studies suggest the importance of the presence of two OH groups in orthosubstitution in the B ring and of an OH in the 3 position. Flavan-type flavonoids behave like their related flavonoids; d-catechin also opposes lysosome disruption. Kaempferol, quercetin, 7,8-dihydroxyflavone and d-catechin inhibit lipoperoxidation occurring in an L fraction incubated with the xanthine oxidase system as ascertained by malondialdehyde (MDA) production. For kaempferol and quercetin, such an inhibition parallels the prevention of NAGase release; this is not the case for the two other compounds where inhibition of NAGase release takes place at a flavonoid concentration lower than that required to oppose MDA production. Morphological observations performed on purified lysosomes confirm the biochemical results. Some flavonoids are also able to prevent release of NAGase caused by the incubation of an L fraction in isoosmotic glucose. Only flavone and hydroxyflavones are effective. It is proposed that the protective effect of flavonoids on lysosomes subjected to oxygen free radicals does not only originate from their scavenger and antilipoperoxidant properties; a more direct action on lysosomal membrane making it more resistant to oxidative aggression has to be considered. The prevention by some flavonoids of lysosome osmotic disruption in isoosmotic glucose could be the result of an inhibition of glucose translocation through the lysosomal membrane.
- Published
- 1992
- Full Text
- View/download PDF
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