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A comparative study of the subcellular distribution of native and deglycosylated gelonin in rat liver and kidney.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2004 Jul 09; Vol. 319 (4), pp. 1299-306. - Publication Year :
- 2004
-
Abstract
- Intravenous injection of gelonin and deglycosylated gelonin led to rapid clearance from the blood. Both molecules distributed similarly in liver and kidney suggesting that they followed the same pathway. Deglycosylation reduced the uptake by a third in liver, but did not affect uptake by kidney. Studies with Triton WR1339 showed a classical lysosomal pathway for both molecules. The deglycosylated molecule was degraded to a greater extent than native gelonin as seen by the presence of acid soluble radioactivity. Cell separation showed that while endothelial cells mainly took up native gelonin, Kupffer cells took up the deglycosylated molecule.
- Subjects :
- Animals
Centrifugation
Glycosylation
Iodine Radioisotopes metabolism
Male
Plant Proteins chemistry
Protein Synthesis Inhibitors chemistry
Rats
Rats, Wistar
Ribosome Inactivating Proteins, Type 1
Subcellular Fractions chemistry
Kidney metabolism
Liver metabolism
Plant Proteins metabolism
Protein Synthesis Inhibitors metabolism
Subcellular Fractions metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0006-291X
- Volume :
- 319
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 15194509
- Full Text :
- https://doi.org/10.1016/j.bbrc.2004.05.117