39 results on '"Surana, S. J."'
Search Results
2. Simultaneous determination of levofloxacin hemihydrate and ambroxol hydrochloride in tablets by thin-layer chromatography combined with densitometry
- Author
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Agrawal, O. D., Shirkhedkar, A. A., and Surana, S. J.
- Published
- 2010
- Full Text
- View/download PDF
3. Antihyperglycemic Activity of Various Fractions of Cassia auriculata Linn. in Alloxan Diabetic Rats
- Author
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Surana, S. J., Gokhale, S. B., Jadhav, R. B., Sawant, R. L., and Wadekar, Jyoti B.
- Subjects
alloxan-induced diabetes ,Cassia auriculata ,antidiabetic activity ,Short Communications ,hydromethanolic extract - Abstract
Present work describes the potent antidiabetic fraction from flowers of Cassia auriculata Linn. Hydromethanolic extract along with its ethyl acetate and n-butanol fractions were evaluated for antidiabetic activity in alloxan-induced diabetes in rats. The n-butanol fraction exhibited significant reduction (p
- Published
- 2008
4. SIMULTANEOUS ESTIMATION OF MOXIFLOXACIN HYDROCHLORIDE AND DEXAMETHASONE SODIUM PHOSPHATE IN BULK AND IN OPHTHALMIC SOLUTION BY RP- HPLC
- Author
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DHUMAL,D. M, SHIRKHEDKAR,A. A, NERKAR,P. P, and SURANA,S. J
- Subjects
RP-HPLC ,Dexamethasone Sodium Phosphate ,Moxifloxacin Hydrochloride - Abstract
A new simple, precise, accurate and selective RP-HPLC method has been developed and validated for simultaneous estimation of Moxifloxacin Hydrochloride (MOX) and Dexamethasone Sodium Phosphate (DSP) in Ophthalmic Solution. The method was carried out on a Qualisil RP C-8 (250 mm x 4.6 mm, 5 µm) column with a mobile phase consisting of Methanol: Water (75:25 v/v) pH adjusted to 3.0 with ortho-phosphoric acid of aqueous phase and flow rate of 1.0 mL min¹. Detection was carried out at 240 nm. The retention time for MOX and DSP was found to be 2.22, and 7.26 min, respectively. The MOX and DSP followed linearity in the concentration range of 10 - 60 µg mL-1 and 2- 12 µg mL-1 with r²= 0.99, respectively. The amounts of both these drugs estimated by proposed method were found to be in good agreement with label claim. The developed method was validated for sensitivity, accuracy, precision, ruggedness and robustness. The LOD and LOQ were found to be 0.30 µg mL-1 and 0.91 µg mL-1 for MOX and 0.10 µg mL-1 and 0.30 µg mL-1 for DSP. The proposed method can be used for routine analysis of both these drugs simultaneously in their combined dosage form.
- Published
- 2012
5. SIMULTANEOUS DETERMINATION OF DOMPERIDONE AND PARABEN PRESERVATIVE IN ORAL FORMULATION: REVERSED-PHASE LIQUID CHROMATOGRAPHIC METHOD.
- Author
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Patel A., Firke S. D., Patil R. R., Kalaskar M. G., Bari S. B., and Surana S. J.
- Subjects
DOMPERIDONE ,LIQUID sodium ,GRADIENT elution (Chromatography) ,SODIUM benzoate ,HIGH performance liquid chromatography ,ELUTION (Chromatography) ,RF values (Chromatography) - Abstract
A novel RP-HPLC method was developed and validated for the determination of compounds in an oral solution. The method describes the determination of domperidone along with sodium methylparaben, sodium propylparaben and sodium benzoate in liquid oral formulation. The chromatographic separations were performed using BDS Hypersil 5 µm C18 column and gradient elution (solvent A: phosphate buffer, pH 3.5 and solvent B: methanol) keeping a flow rate of 1.5 mL/min. Detection was done at dual wavelength (232 nm for domperidone and sodium benzoate, and 257 nm for sodium methylparaben and sodium propylparaben). Analysis time was <17 min. The retention times for domperidone, sodium benzoate, sodium methylparaben and sodium propylparaben were found to be 10.0, 6.5, 8.0, and 13.5 min, the calibration curves for domperidone, sodium benzoate, sodium methylparaben and sodium propylparaben were found to be linear in the range of 250-750, 50-150, 50-150, and 5-15 µg/mL, respectively. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
6. Forskolin: Upcoming antiglaucoma molecule.
- Author
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Wagh, V. D., Patil, P. N., Surana, S. J., and Wagh, K. V.
- Subjects
DRUG approval ,MEDICINAL plants ,GLAUCOMA ,ULCERS ,ANIMAL experimentation ,DRUG storage - Abstract
Forskolin is the first pharmaceutical drug and product derived from a plant to be approved in India by the DCGI in 2006. Forskolin (7beta-acetoxy-8, 13-epoxy-1a, 6β, 9a-trihydroxy-labd-14-en-11-one) is a diterpenoid isolated from plant Coleus forskohlii (Lamiaceae). It is a lipid-soluble compound that can penetrate cell membranes and stimulates the enzyme adenylate cyclase which, in turn, stimulates ciliary epithelium to activate cyclic adenosine monophosphate, which decreases intraocular pressure (IOP) by reducing aqueous humor inflow. The topical application of forskolin is capable of reducing IOP in rabbits, monkeys, and humans. In its drug interactions, forskolin may act synergistically with epinephrine, ephedrine and pseudoephedrine. Whereas the effects of anti-clotting medications like warfarin, clopidogre, aspirin, anoxaparin, etc., may be enhanced by forskolin. Forskolin is contraindicated in the medications for people with ulcers as forskolin may increase acid level. Forskolin has a very good shelf-life of five years. Recently, its Ophthalmic inserts and in situ gels for sustained and delayed-release drug delivery systems were tested in New Zealand Albino Rabbits for its antiglaucoma efficacy. This drug review explains Forskolin as a drug, its antiglaucoma potential and recent findings of forskolin as an antiglaucoma agent. The literature search method used for this review was different databases and search engines like PubMed, International Pharmaceutical Abstracts, Google, Medicinal and Aromatic Plants (MAPA). [ABSTRACT FROM AUTHOR]
- Published
- 2012
7. Quantitative Determination of Ciprofibrate in Tablets by Derivative UV Spectroscopy and RP-HPLC Method.
- Author
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JAIN, P. S., JIVANI, H. N., KHATAL, R. N., and SURANA, S. J.
- Subjects
BUTYRATES ,DRUG tablets ,DRUG derivatives ,DRUG development ,QUANTITATIVE research ,ULTRAVIOLET spectroscopy ,HIGH performance liquid chromatography - Abstract
A derivative UV spectrophotometric and a reversed phase high-performance liquid chromatographic method for determination of ciprofibrate in tablets was developed. The first-order derivative UV spectrophotometric method found to be accurate with 100.57±0.97 recovery and precise with a coefficient of variation of 1.44. These results were compared to those obtained by reference methods, zero-order UV spectrophotometric method and a reversed-phase high-performance liquid chromatography method. A reversed-phase C8 column with methanol:water (90:10, pH 3.7) mobile phase was used and the detector wavelength was set at 232 nm. Calibration solutions used in HPLC were ranging from 2 to 12 µg/ml. An ANOVA test (P = 0.0226, F = 4.935) showed that the results obtained with the derivative UV spectrophotometric method were comparable to those obtained using reference methods. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF
8. Development and Validation of HPTLC Method for Simultaneous Determination of Amlodipine Besylate and Metoprolol Succinate in Bulk and Tablets.
- Author
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JAIN, P. S., PATEL, M. K., BARI, S. B., and SURANA, S. J.
- Subjects
DRUG development ,AMLODIPINE ,METOPROLOL ,DRUG tablets ,DRUG dosage ,HIGH performance liquid chromatography ,DATA analysis - Abstract
A simple, selective, precise high-performance thin-layer chromatographic method for simultaneous determination of amlodipine besylate and metoprolol succinate in bulk and pharmaceutical combined dosage form was developed and validated. The method employed HPTLC aluminum plates precoated with silica gel 60F-254 (10x10) as the stationary phase. The solvent system consisted of toluene:ethyl acetate:methanol:triethylamine (4:1:1:0.4 v/v/v). The system was found to give a compact spot for amlodipine besylate (R
f = 0.39±0.02) and metoprolol succinate (Rf = 0.59±0.02). Densitometric analysis of amlodipine besylate and metoprolol succinate was carried out in the absorbance mode at 254 nm. Linear regression analysis data for the calibration plots showed good linear relationship with r² = 0.9990±0.0013 with respect to peak area in the concentration range 400-1400 ng per spot for amlodipine besylate and r² = 0.9993±0.0013 with respect to peak area in the concentration range 3800-13300 ng per spot for metoprolol succinate. The method was validated for precision, recovery and robustness. The limits of detection and quantitation were 39.99 and 121.20 ng per spot for amlodipine besylate and 234.31 and 710.03 ng per spot for metoprolol succinate, respectively. Statistical analysis proved that the method is selective, precise and accurate for the estimation of amlodipine and metoprolol. [ABSTRACT FROM AUTHOR]- Published
- 2012
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- View/download PDF
9. Stability-Indicating RP-TLC/Densitometry Determination of Raloxifene Hydrochloride in Bulk Material and in Tablets.
- Author
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Shirkhedkar, A. A., Rajput, J. K., Rajput, D. K., and Surana, S. J.
- Abstract
A stability-indicating RP-TLC/Densitometry method for analysis of Raloxifene hydrochloride both in bulk material and in tablets was developed and validated. Densitometric analysis of Raloxifene hydrochloride was carried out at 311 nm on TLC aluminium plates precoated with silica gel 60RP-18 F
254 S as the stationary phase and methanol :water : ammonia (95 : 05 : 0.1 v/v) as mobile phase. Raloxifene hydrochloride was well resolved at Rf 0.55 ± 0.02. The linear regression analysis data for the calibration plots showed good linear relationship with r² = 0.9969 ± 0.0015 with respect to peak area in the concentration range 100-600 ng per band. The mean value ± SD of slope and intercept was found to be 15.05 ± 0.44 and 201.9 ± 29.58 with respect to peak area. The limits of detection and quantification were 9.27 ng and 27.10 ng, respectively. Raloxifene hydrochloride was subjected to acid and alkali hydrolysis, oxidation, dry heat, and photodegradation. The drug underwent degradation under basic and oxidation conditions. This indicates that the drug is susceptible to alkali hydrolysis and oxidation. The proposed developed RPTLC/ Densitometry method can be applied for identification and quantitative determination of Raloxifene hydrochloride in bulk material and tablets. [ABSTRACT FROM AUTHOR]- Published
- 2012
- Full Text
- View/download PDF
10. Development and validation of zero and first-order derivative area under curve spectrophotometric methods for the determination of entacapone in bulk material and in tablets.
- Author
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Chalikwar, S. S., Shirkhedkar, A. A., Bagul, M. A., Jain, P. S., and Surana, S. J.
- Subjects
SPECTROPHOTOMETRY ,ENTACAPONE ,DRUG tablets ,DILUTION ,BULK solids - Abstract
Aim: The aim of this work is to establish two simple, economical, and rapid spectrophotometric methods for the quantification of entacapone in bulk material and in tablets. Further, this study is designed to validate the developed methods as per ICH guidelines. Materials and Methods: In Methods I and II, a stock standard solution was prepared by dissolving 10 mg of entacapone in 100 mL of 10% v/v acetonitrile to obtain a concentration of 100 µg/mL. After suitable dilution, 10 µg/mL of entacapone was prepared and scanned in the UV-visible range 500-200 nm; entacapone showed a maximum absorbance at 384.40 nm. In Method I, area under curve (AUC) of the zero-order spectrum was recorded between 348.00 and 410.20 nm. While, in Method II, zero-order spectra were derivatized into first-order, and the AUC was recorded between 386.40 and 460.20 nm. For a linearity study, series of dilutions were prepared from stock solutions. Results: In Method I, and II, entacapone followed linearity in the concentration range of 2-12 µg/mL and 5-30 µg/mL with (r²>0.999). The amounts of entacapone estimated by both these methods were found to be 99.24 ± 0.054 and 98.68 ± 1.04, respectively. Conclusion: The developed methods are simple, precise, rugged, robust, and economical. Both these methods can be used for routine analysis of entacapone from its tablet formulation. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF
11. Determination of Phyllanthin and Gallic Acid In Herbal Hepatoprotective Formulation By TLC-Densitometry Analysis.
- Author
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Tatiya, A. U., Patil, R. P., Sutar, M. P., Shirkhedkar, A. A., and Surana, S. J.
- Subjects
GALLIC acid ,DENSITOMETRY ,SILICA gel ,FORMIC acid ,ETHYL acetate ,REGRESSION analysis - Abstract
Introduction: Phyllanthus niruri, Andrographis paniculata and Embilica officinalis are most important and high value medicinal plant known for its hepatoprotective activity. Several analytical methods including HPTLC and HPLC are reported for respective phytoconstituents. However, there is no simultaneous estimation of phyllanthin and gallic acid reported. The Objective of present work is to develop and validate HPTLC method for simultaneous determination of phyllanthin and gallic acid in polyherbal hepatoprotective formulation. Method: The method is employed TLC alumina plates precoated with silica gel 60F-254 as a stationary phase and toluene: ethyl acetate: formic acid (5:3.5:0.5 v/v/v) as a mobile phase. The standard markers namely phyllanthin and galic acid were identified in polyherbal formulation containing P.niruri and E.officinalis by R
f value. Densitometric analysis of phyllanthin and gallic acid was carried out at 254nm. Results: The precision of method was confirmed by relative standard deviation (RSD) which was lower than the 2%. This method was found to give proper separation of phyllanthin and gallic acid. The linear regression data for calibration plot shown good relationship with r2 =0.998 (phyllanthin) and 0.9991(Gallic acid) in the concentration range 5-9 μg/band. The method was validated for precision, accuracy, sensitivity, and specificity. Conclusion: statistical analysis proves that method is accurate and reproducible. The method is economical and can be employed for routine analysis of marketed polyherbal hepatoprotective formulation containing P.niruri and E.officinalis. [ABSTRACT FROM AUTHOR]- Published
- 2011
- Full Text
- View/download PDF
12. Acute Oral Toxicity of Abelmoschus manihot and Wrightia tinctoria in Mice.
- Author
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Jain, P. S., Bari, S. B., and Surana, S. J.
- Subjects
MALVACEAE ,APOCYNACEAE ,LABORATORY mice ,MEDICINAL plants ,THERAPEUTICS - Abstract
Abelmoschus manihot and Wrightia tinctoria, belonging to the botanical family Malvaceae and Apocynaceae, have been traditionally used by the locals in India for treatment of various ailments. The current study reports the outcome of acute oral toxicity investigation of Abelmoschus manihot and Wrightia tinctoria, on ICR mice. No mortalities or evidence of adverse effects have been observed in ICR mice following acute oral administration at the highest dose of 2500 mg/ kg crude extracts of Abelmoschus manihot and Wrightia tinctoria. This is the first report on the acute oral toxicity of Abelmoschus manihot and Wrightia tinctoria and the findings of this study are in agreement with those of in vitro experiments and thus provide scientific validation on the use of the leaves of Abelmoschus manihot and Wrightia tinctoria. [ABSTRACT FROM AUTHOR]
- Published
- 2011
- Full Text
- View/download PDF
13. Spectrophotometric determination of ethacridine lactate in infusion.
- Author
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Jain, Pritam S. and Surana, S. J.
- Subjects
- *
SPECTROPHOTOMETRY , *LACTATES , *MEDICAL prescriptions , *DISTILLED water , *DOSAGE forms of drugs - Abstract
Aim: A simple, rapid, selective, accurate, and precise UV spectrophotometric method has been developed for the estimation of ethacridine lactate from bulk and pharmaceutical formulation. Materials and Methods: Appropriate aliquot portions of stock standard solution of ethacridine lactate were transferred into five separate 10 ml volumetric flasks, and the volume was adjusted to the mark with double distilled water to obtain concentrations of 0.2, 0.4, 0.6, 0.8, 1.0, and 1.2 µg/ml. The λmax of ethacridine lactate in double distill water was found to be 271 nm with an apparent molar absorptivity of 59.781 x 10³ l/mol cm. The drug follows linearity in the concentration range 2-12 µg/ml with a correlation coefficient value of 0.998. Results: The proposed method was applied to pharmaceutical formulation and % amount of drug estimated 99.71% was found to be in good agreement with the label claim. The accuracy of the method was checked by recovery experiment performed at three different levels, i.e., 80%, 100%, and 120%. The % recovery was found to be in the range 99.26-100.25%. The low values of % RSD are indicative of the accuracy and reproducibility of the method. The precision of the method was studied as intraday, interday variations and repeatability. The % RSD value less than 2 indicates that the method is precise. Ruggedness of the proposed method was studied with the help of two analysts. Conclusion: The results indicated that the method could be used for the routine estimation of ethacridine lactate from tablet formulations. [ABSTRACT FROM AUTHOR]
- Published
- 2011
- Full Text
- View/download PDF
14. Development and validation of RP-HPLC method for estimation of ethacridine lactate in bulk and in pharmaceutical formulation.
- Author
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Jain, P. S., Jivani, H. N., Khatal, R. N., and Surana, S. J.
- Subjects
METHANOL ,EXCIPIENTS ,HIGH performance liquid chromatography ,DRUGS ,ALCOHOLS (Chemical class) - Abstract
A new simple, precise, accurate and selective RP-HPLC method has been developed and validated for estimation of Ethacridine lactate in pharmaceutical formulation. The method was carried out on a Qualisil RP C-18 (250 mm x 4.6 mm, 5 µm) column with a mobile phase consisting of methanol: water (60:40 v/v), pH adjusted to 2.8 with orthophosphoric acid and low rate of 1.0 mL/min. Detection was carried out at 271 nm. The retention time for ethacridine lactate was found to be 4.41 min. The ethacridine lactate followed linearity in the concentration range of 2- 12 µg/mL (r² = 0.9980). The amount of the drug estimated by proposed method was found to be in good agreement with label claim. The developed method was validated for sensitivity, accuracy, precision, ruggedness and robustness. The LOD and LOQ were found to be 0.11 and 0.33 µg. The proposed method can be used for routine analysis of ethacridine lactate in bulk drug and pharmaceutical formulation. [ABSTRACT FROM AUTHOR]
- Published
- 2011
- Full Text
- View/download PDF
15. Development and Validation of Stability-Indicating HPTLC Determination of Tamsulosin in Bulk and Pharmaceutical Dosage Form.
- Author
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Bari, S. B., Bakhshi, A. R., Jain, P. S., and Surana, S. J.
- Abstract
A simple, economic, selective, precise, and stability-indicating high-performance thin-layer chromatographic method for analysis of tamsulosin hydrochloride, both as a bulk drug and in formulations, was developed and validated according to ICH guidelines. The method employed HPTLC aluminiumplates precoated with silica gel 60F-254 as the stationary phase while the solvent system consisted of toluene : methanol : triethylamine (3.5 : 1.2 : 0.2v/v). The systemwas found to give compact spot for drug (R
f value of 0.52 ± 0.02). Densitometric analysis of tamsulosin was carried out in the absorbance mode at 280 nm. The linear regression analysis data for the calibration plots showed good linear relationship, r2 = 0.9982 ± 0.0012, with respect to peak area in the concentration range 400-2400 ng per spot. Themean value ± SD of slope and intercept were 2.6553±0.0173 and 777.7± 74.8with respect to peak area. The method was validated for precision, recovery, and robustness. The limits of detection and quantitation were 20.49 and 62.10 ng per spot, respectively. Tamsulosin was subjected to hydrolysis, oxidation, and thermal degradation which indicate the drug is susceptible to hydrolysis, oxidation, and heat. Statistical analysis proves that themethod is repeatable, selective, and accurate for the estimation of tamsulosin. [ABSTRACT FROM AUTHOR]- Published
- 2011
- Full Text
- View/download PDF
16. APPLICATION OF ACUPUNCTURE THERAPY IN TYPE 2 DIABETES MELLITUS PATIENTS.
- Author
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Ingle, P. V., Samdani, N. R., Patil, P. H., Pardeshi, M. S., and Surana, S. J.
- Published
- 2011
17. Diuretic activity of squamate mistletoe, Viscum angulatum.
- Author
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Jadhav, R. B., Bhatnagar, S. P., and Surana, S. J.
- Subjects
VISCACEAE ,BLOOD circulation disorders ,SANTALALES ,CARBONIC anhydrase ,POTASSIUM - Abstract
Viscum angulatum Heyne ex DC (Viscaceae) is a leafless hemiparasitic shrub traditionally used in Asian countries for the treatment of hypertension. In the present study, the methanol extract of the whole plant was examined for diuretic activity in rats. The activity was evaluated using parameters such as urine volume after 5, 19, and 24 h and urine sodium, potassium, and chloride concentrations. The extract was further scrutinized for polyphenolic compounds and triterpenoids. The extract demonstrated a significant increase in and dose-dependent effect on urine excretion volume in comparison to the normal group in the tested range of 100–400 mg/kg. The extract demonstrated comparable saluretic and higher natriuretic activity (Na
+ /K+ ) compared to the furosemide treated group. However, the Cl− /Na+ + K+ ratio, which indicates carbonic anhydrase mediated activity, remained unaffected. HPLC and quantitative analysis of the extract revealed that polyphenolic compounds and the triterpenoid, oleanolic acid, are the major phytochemicals, and are proposed to be responsible for the observed diuretic effect. Oleanolic acid has been reported to possess diuretic activity with significant potassium loss in rats. In contrast to pure oleanolic acid, the extract demonstrated a valuable potassium-sparing effect. This suggests modulation of the diuretic effect of oleanolic acid by polyphenolics present in the extract. The observed dose-dependent potassium-sparing diuretic effect is a hereto unreported property of this plant. [ABSTRACT FROM AUTHOR]- Published
- 2010
- Full Text
- View/download PDF
18. Antioxidant Properties of the Methanol Extract of the Wood and Pericarp of Caesalpinia decapetala.
- Author
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Pawar, C. R. and Surana, S. J.
- Subjects
- *
ANTIOXIDANTS , *CHEMICAL inhibitors , *CAESALPINIA , *NITRIC oxide , *PLANT extracts , *HERBAL medicine , *PEROXIDATION , *ALTERNATIVE medicine , *CASCARA sagrada - Abstract
The antioxidant activities of the methanol extracts from the wood and pericarp of Caesalpinia decapetala (Roth) Alston (Caesalpiniaceae) were assessed in efforts to validate the herb. The antioxidant activity of the plant has been studied using its ability to scavenger DPPH, superoxide radicals, and nitric oxide radical along with its ability to inhibit lipid peroxidation. The antioxidant activity and phenolic content of the pericarp as determined by the DPPH, superoxide radical, nitric oxide radical, total phenols, the flavonoids, and total flavonols were higher than that of the wood. Analysis of plant extracts revealed a high amount of polyphenols and flavonoids suggesting a possible role of these phytoconstituents in the antioxidant property. Moreover, the results were observed in a concentration and dose dependent manner. Studies clearly indicate that the C. decapetala has significant antioxidant activity. [ABSTRACT FROM AUTHOR]
- Published
- 2010
- Full Text
- View/download PDF
19. Topical Delivery of Flurbiprofen from Pluronic Lecithin Organogel.
- Author
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PANDEY, M. S., BELGAMWAR, V. S., and SURANA, S. J.
- Subjects
LECITHIN ,PHARMACEUTICAL gels ,FLURBIPROFEN ,DRUG administration ,SORBIC acid - Abstract
The purpose of this research is to formulate and evaluate the suitability of pluronic lecithin organogels containing flurbiprofen for topical application. Four formulations were developed using flurbiprofen, lecithin, Pluronic F127, isopropyl palmitate, water, sorbic acid and potassium sorbate were coded as FL1, FL2, FL3 and FL4. All the formulations carried 30% w/w of lecithin phase and 70% w/w of Pluronic phase. The formulated organogels were evaluated for appearance and feel psychorheologically, in vitro diffusion study, drug content, viscosity and pH. Release of flurbiprofen from all formulations was monitored via dialysis membrane-70 and Wistar rat skin as a semipermeable membrane into phosphate buffer saline (0.2 M, pH 7.4) using Keshary-Chien diffusion cell. The viscosities of different formulations were determined by using Brookfield Viscometer at 25°. An attempt has been made to explore the potential of pluronic lecithin organogels for topical delivery of flurbiprofen. [ABSTRACT FROM AUTHOR]
- Published
- 2009
20. Synthesis of 2-Substituted Methylthieno [2,3- d]Pyrimidin-4(3H)-ones and Evaluation for Antihyperlipidemic Activity.
- Author
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Murumkar, P. R., Fursule, R. A., Jain, K. S., and Surana, S. J.
- Subjects
ANTICHOLESTEREMIC agents ,NIACIN ,ESTERS ,STATINS (Cardiovascular agents) ,GUM arabic - Abstract
A series of nicotinyl 4-oxathieno[2,3-d]pyridine-2-ylmethyl esters, title compounds were synthesized. Compound IIIa was subjected to in vivo antihyperlipidemic activity in Wistar rats. The activity exhibited by synthesized compound was found to be less as compared to that produced by a standard, atorvastatin, administered in the form of suspension in 2% gum acacia. The activity was even lesser than that produced by the lead IIa. [ABSTRACT FROM AUTHOR]
- Published
- 2007
- Full Text
- View/download PDF
21. Validated HPTLC Method for Simultaneous Determination of Quinapril Hydrochloride and Hydrochlorothiazide in a Tablet Dosage Form.
- Author
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BHAVAR, GIRIJA B., CHATPALLIWAR, V. A., PATIL, D. D., and SURANA, S. J.
- Subjects
QUINAPRIL ,HYDROCHLORIC acid ,DIURETICS ,HIGH performance liquid chromatography ,EXCIPIENTS ,SILICA gel - Abstract
Quinapril hydrochloride and hydrochlorothiazide were simultaneously determined by HPTLC in pharmaceutical formulations. The drugs were separated on silica gel 60 F
254 plates using suitable combination of solvents as mobile phase. The validation parameters, tested in accordance with the requirements of ICH guidelines, prove the suitability of methods. [ABSTRACT FROM AUTHOR]- Published
- 2008
22. Simultaneous Derivative and Multi-Component Spectrophotometric Determination of Drotaverine Hydrochloride and Mefenamic Acid in Tablets.
- Author
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Dahivelkar, P. P., Mahajan, V. K., Bari, S. B., Shirkhedkar, A. A., Fursule, R. A., and Surana, S. J.
- Subjects
HYDROCHLORIC acid ,MEFENAMIC acid ,DRUG tablets ,SPECTROPHOTOMETRY ,DRUGS - Abstract
Two new, simple, accurate and economical spectrophotometric methods have been developed for simultaneous estimation of drotaverine hydrochloride and mefenamic acid in two-component tablet formulation. The methods employed are, first derivative spectrophotometry, using zero crossing technique and multicomponent analysis. Both the drugs obey the Beer's law in the concentration range employed for these methods. The results of analysis are validated by statistical evaluation and recovery studies. [ABSTRACT FROM AUTHOR]
- Published
- 2007
23. RP-HPLC Estimation of Paracetamol and Valdecoxib in Combined Dosage Form.
- Author
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Bhavsar, A. S., Talele, G. S., Fursule, R. A., and Surana, S. J.
- Subjects
LIQUID chromatography ,ACETAMINOPHEN ,DRUG tablets ,METHANOL ,PHOSPHATES - Abstract
A reverse phase high performance liquid chromatography method was developed for simultaneous estimation of paracetamol and valdecoxib in tablet formulation. The separation was achieved by Luna C
18 column and methanol: phosphate buffer pH 3.5 (60:40 v/v) as eluent, at a flow rate of 1.0 ml/min. Detection was carried out at 242 nm. Etoricoxib was used as an internal standard. The retention time of PAR and VAL was found to be 3.01 and 8.51 min, respectively. The method was validated for linearity, accuracy and precision. Linearity for paracetamol and valdecoxib were in the range 25-150 µg/ml and 1-6 µg/ml, respectively. The developed method was found to be accurate, precise, and selective for simultaneous estimation of paracetamol and valdecoxib in tablets. [ABSTRACT FROM AUTHOR]- Published
- 2006
24. RP-HPLC Estimation of Tizanidine HCl and Valdecoxib in Combined Dosage Forms.
- Author
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Bhavsar, A. S., Talele, G. S., Fursule, R. A., and Surana, S. J.
- Subjects
LIQUID chromatography ,DRUG analysis ,DRUG tablets ,ACETONITRILE ,PHOSPHATES - Abstract
A reverse phase high performance liquid chromatography method was developed for simultaneous estimation of tizanidine HCl and valdecoxib in tablet formulation. The separation was achieved by Luna C
18 column and acetonitrile: phosphate buffer pH 3.5 (50:50 v/v) as eluent, at a flow rate of 0.5 ml/min. Detection was carried out at 227 nm. Etoricoxib was used as an internal standard. The retention time of tizanidine and valdecoxib was found to be 4.43 and 16.60 min, respectively. The method was validated for linearity, accuracy and precision. Linearity for tizanidine and valdecoxib were in the range 0.4-2.0 µg/ml and 4-20 µg/ml, respectively. The developed method was found to be accurate, precise and selective for simultaneous estimation of tizanidine and valdecoxib in tablets. [ABSTRACT FROM AUTHOR]- Published
- 2006
25. Spectrophotometric Determination of Carvedilol from Bulk and Formulations.
- Author
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JAIN, P. S., TALELE, G. S., TALELE, S. G., and SURANA, S. J.
- Published
- 2005
26. QUANTITATIVE DETERMINATION OF LEVOFLOXACIN HEMIHYDRATE IN BULK AND TABLETS BY UV-SPECTROPHOTOMETRY AND FIRST ORDER DERIVATIVE METHODS.
- Author
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Shirkhedkar, A. A. and Surana, S. J.
- Abstract
Two Simple, rapid, accurate and economical 'UV Spectrophotometry' and 'First Order Derivative' methods have been developed for determination of levofloxacin hemihydrate in bulk and tablets. In (10% v/v) acetonitrile, the ?max of the drug was found to be 288 nm. The same spectrum was derivatised into first order derivative, using UV probe software of instrument (Shimadzu-2450), at Δλ = 4. The amplitude of the trough was recorded at 297 nm. In both the proposed methods, levofloxacin hemihydrate follows linearity in the concentration range 2 - 12 μg/ml with a correlation coefficient of 0.9999. Assay results were in good agreement with label claim. The methods were validated statistically and by recovery studies. The relative standard deviation were found to be less than 2% with excellent precision and accuracy. [ABSTRACT FROM AUTHOR]
- Published
- 2009
27. Simultaneous Spectrophotometric Estimation of Atorvastatin Calcium and Ezetimibe in Tablets.
- Author
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Sonawane, S. S., Shirkhedkar, A. A., Fursule, R. A., and Surana, S. J.
- Subjects
SPECTROPHOTOMETRY ,CALCIUM ,DRUG tablets ,METHANOL ,ABSORPTION spectra - Abstract
A simple, accurate, economical and reproducible procedure for simultaneous estimation of atorvastatin calcium and ezetimibe in their tablet dosage form has been developed using, Q-absorbance equation. The method involves, the absorbance measurement at 235.5 nm (iso-absorptive point) and 246.0 nm (λmax of atorvastatin calcium), in methanol. Both the drugs, obey Beer-Lambert's law in the concentration range of 5-25 µg/ml. The results of analysis have been validated statistically and by recovery studies. [ABSTRACT FROM AUTHOR]
- Published
- 2007
28. ChemInform Abstract: Synthesis of 2,3-Disubstituted-quinazolin-4-(3H)-ones.
- Author
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Patil, D. A., Patil, P. O., Patil, G. B., and Surana, S. J.
- Published
- 2011
- Full Text
- View/download PDF
29. DEVELOPMENT AND VALIDATION OF STABILITY-INDICATING RP-HPLC METHOD FOR DETERMINATION OF OLMESARTAN MEDOXOMILE IN PHARMACEUTICAL DOSAGE FORM AND IDENTIFICATION, CHARACTERIZATION OF ALKALINE DEGRADATION IMPURITY OF OLMESARTAN MEDOXOMILE DRUG SUBSTANCE AS WELL AS DRUG PRODUCT.
- Author
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JAIN, P. S., CHAUDHARI, A. J., and SURANA, S. J.
- Subjects
- *
HIGH performance liquid chromatography , *DOSAGE forms of drugs , *ANGIOTENSIN II , *ANGIOTENSIN receptors , *ANTIHYPERTENSIVE agents , *STABILITY theory , *CARBOXYLIC acids - Abstract
Olmesartan medoxomil (OLME) belongs to a group of angiotensin II receptor blockers used as an antihypertensive agent and is currently being used for prevention of hypertension. This paper describes the validation and development of stability indicating RP-HPLC method for the determination of OLME in the presence of its degradation products generated from forced degradation study and characterization of degradation product (impurity). The assay involved gradient elution of OLME and an LC GC BDS C18 column (250 mmx4.5 mm, 5 μm particle size) was employed for loading the sample. The mobile phase A consisted of 7 ml triethylamine in 1000 ml water (pH adjusted to 3.0 with orthophosphoric acid) and B contained acetonitrile. Quantification was achieved with photodiode array detection at 257 nm. The chromatographic separation was obtained with a retention time of 6.72 min, and the method was linear in the range 50-150 µg/ml. The method was validated according to the ICH guidelines with respect to linearity, precision, accuracy, limit of detection (LOD), limit of quantification (LOQ), specificity and robustness. Impurity found in stressed and stability studies of olmesartan medoxomil in both drug substance and drug product are described. This degradation product is identified as 1-(biphenyl-4-ylmethyl)-1H-imidazole-5-carboxylic acid. An alkaline degradation pathway of Olmesartan Medoxomil, for the formation of this degradation product, has been proposed and the degradation product was characterized. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF
30. VALIDATION OF A DISSOLUTION METHOD WITH RP-HPLC ANALYSIS FOR PERINDOPRIL ERBUMINE AND INDAPAMIDE COMBINATION TABLET.
- Author
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Jain, P. S., Badreshiya, P. R., Chalikwar, S. S., Todarwal, A. A., and Surana, S. J.
- Subjects
- *
DISSOLUTION (Chemistry) , *INDAPAMIDE , *HIGH performance liquid chromatography , *DRUG tablets , *SOLUTION (Chemistry) , *DRUG analysis - Abstract
A dissolution method with high performance liquid chromatography (HPLC) analysis was validated for perindopril erbumine and indapamide in combination tablet formulation. The method was validated to meet requirements for a global regulatory filing and this validation included specificity, linearity, accuracy, precision, range, robustness and solution stability studies. The dissolution method, which uses a USP apparatus 1 with basket rotating at 100 rpm, 1000 ml of phosphate buffer, pH 6.8, as the dissolution medium, and reversed-phased HPLC was carried out at 50 °C on a 4.6 mmx250 mmx5 µm cyano column that contained USP packing L1 with acetonitrile:buffer 40:60 (v/v), pH 2.8, as mobile phase. UV detector was set at 225 nm. The method was found to be selective, linear, accurate and precise in the specified ranges. Intra-day and inter--day variability for method was <2% RSD. This method was successfully used for quantification of perindopril erbumine and indapamide combination tablet formulations. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF
31. STABILITY-INDICATING HPLC DETERMINATION OF CIPROFIBRATE IN BULK DRUG AND PHARMACEUTICAL DOSAGE FORM.
- Author
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Jain, P. S., Jivani, H. N., Khatal, R. N., and Surana, S. J.
- Subjects
- *
DRUG stability , *HIGH performance liquid chromatography , *DOSAGE forms of drugs , *CHROMATOGRAPHIC analysis , *HYDROLYSIS , *PHOTOLYSIS (Chemistry) - Abstract
A novel stability-indicating high-performance liquid chromatographic assay method was developed and validated for quantitative determination of ciprofibrate in bulk drugs and in pharmaceutical dosage form in the presence of degradation products. An isocratic, reversed phase HPLC method was developed to separate the drug from the degradation products, using an Ace5-C18 (250 mmx4.6 mm, 5 µm) advanced chromatography column, and methanol and water (90:10 v/v) as a mobile phase. The detection was carried out at a wavelength of 232 nm. The ciprofibrate was subjected to stress conditions of hydrolysis (acid and base), oxidation, photolysis and thermal degradation. Degradation was observed for ciprofibrate in base, in acid and in 30% H2O2. The drug was found to be stable in the other stress conditions attempted. The degradation products were well resolved from the main peak. The percentage recovery of ciprofibrate was from (98.65 to 100.01%) in the pharmaceutical dosage form. The developed method was validated with respect to linearity, accuracy (recovery), precision, system suitability, specificity and robustness. The forced degradation studies prove the stability indicating power of the method. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF
32. Stability-indicating method for simultaneous estimation of olmesartan medoxomile, amlodipine besylate and hydrochlorothiazide by RP-HPLC in tablet dosage form.
- Author
-
Jain PS, Patel MK, Gorle AP, Chaudhari AJ, and Surana SJ
- Subjects
- Amlodipine chemistry, Chromatography, Reverse-Phase methods, Drug Stability, Hydrochlorothiazide chemistry, Imidazoles chemistry, Linear Models, Olmesartan Medoxomil, Reproducibility of Results, Sensitivity and Specificity, Tablets analysis, Tablets chemistry, Tetrazoles chemistry, Amlodipine analysis, Chromatography, High Pressure Liquid methods, Hydrochlorothiazide analysis, Imidazoles analysis, Tetrazoles analysis
- Abstract
A simple, specific, accurate and precise stability-indicating reversed-phase high-performance liquid chromatographic method was developed for simultaneous estimation of olmesartan medoxomile (OLME), amlodipine besylate (AMLO) and hydrochlorothiazide (HCTZ) in tablet dosage form. The method was developed using an RP C18 base deactivated silica column (250 × 4.6 mm, 5 µm) with a mobile phase consisting of triethylamine (pH 3.0) adjusted with orthophosphoric acid (A) and acetonitrile (B), with a timed gradient program of T/%B: 0/30, 7/70, 8/30, 10/30 with a flow rate of 1.4 mL/min. Ultraviolet detection was used at 236 nm. The retention times for OLME, AMLO and HCTZ were found to be 6.72, 4.28 and 2.30, respectively. The proposed method was validated for precision, accuracy, linearity, range, robustness, ruggedness and force degradation study. The calibration curves of OLME, AMLO and HCTZ were linear over the range of 50-150, 12.5-37.5 and 31-93 µg/mL, respectively. The method was found to be sensitive. The limits of detection of OLME, AMLO and HCTZ were determined 0.19, 0.16 and 0.22 µg/mL and limits of quantification of OLME, AMLO and HCTZ were determined 0.57, 0.49 and 0.66, respectively. Forced degradation study was performed according to International Conference on Harmonization guidelines.
- Published
- 2012
- Full Text
- View/download PDF
33. Synthesis of 2, 3-disubstituted-Quinazolin-4-(3H)-ones.
- Author
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Patil DA, Patil PO, Patil GB, and Surana SJ
- Subjects
- Benzoxazines chemistry, Diamide chemistry, Microwaves, Quinazolinones chemistry, Sulfuric Acids chemistry, Quinazolinones chemical synthesis
- Abstract
The present review covers a concise account of the synthesis of bioactive 2, 3-disubstituted-quinazoline-4(3H)-ones and the recent developments in the area of versatile quinazolinones with a special emphasis on new synthetic routes and strategies.
- Published
- 2011
- Full Text
- View/download PDF
34. Optimizing conditions for gallic acid extraction from Caesalpinia decapetala wood.
- Author
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Pawar CR and Surana SJ
- Subjects
- Chromatography, Thin Layer, Ethanol, Gallic Acid analysis, India, Phenols analysis, Plant Extracts analysis, Solvents, Water, Caesalpinia chemistry, Gallic Acid isolation & purification, Wood chemistry
- Abstract
Caesalpinia decapetala is a wild plant found in the Sub-Himalayan tract and planted in hedges throughout in India. The bark of Caesalpinia decapetala is a rich source of tannins. It has been used in treatment of jaundice, stomach disorders and biliousness. The leaves and root are used as purgative and emmenagogue. The process described in this paper outline the extraction of gallic acid from Caesalpinia decapetala which is used as antioxidant and anti-inflammatory. Optimization of various solvent extraction parameters was performed to assess maximum yield of gallic acid from Caesalpinia decapetala wood. The extraction parameters optimized are solvent, temperature and time for extraction. Optimization was carried out by performing different sets of experiments. The most suitable conditions for extraction of gallic acid were found to be extraction at temperature (65-70(o)C), extraction time 48 hours and solvent composition Ethanol: Water (70:30). At these optimum extraction parameters the maximum yield of gallic acid obtained is 17.85%.
- Published
- 2010
35. Diuretic and natriuretic activity of two mistletoe species in rats.
- Author
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Jadhav N, Patil CR, Chaudhari KB, Wagh JP, Surana SJ, and Jadhav RB
- Abstract
In different cultural groups, the hemiparasitic plants of the families Loranthaceae and Viscaceae (mistletoes) are frequently used in the treatment of hypertension and/or as diuretic agents. However, it remains unclear as to what commonality makes them diuretic agents or a remedy for hypertension. In this article, the diuretic activity of methanol extracts of Viscum articulatum (VA) Burm. f. and Helicanthus elastica (HE) (Ders.) Dans. in rats is reported. The extracts were administered orally at doses of 100, 200 and 400 mg/kg to rats that had been fasted and deprived of water for 18 hours. Investigations were carried out for diuretic, saluretic and natriuretic effects. The polyphenolic and triterpenoid contents were determined quantitatively using chemical assays and high performance liquid chromatography (HPLC) analysis, respectively. The extracts of VA and HE demonstrated significant and dose-dependent diuretic activity in rats. It was found that while VA mimics the furosemide pattern, HE demonstrated a dose-dependent increase in diuresis, along with an increase in potassium-sparing effects. Phytochemical analysis revealed that polyphenolics and triterpenoids, such as oleanolic acid and lupeol, are the major phytochemicals involved. It was also found that in different combinations, these phytochemicals differed in the way they influenced the electrolyte excretion. A higher content of polyphenolics in association with lower triterpenoid content was found to favor potassium-sparing effects.
- Published
- 2010
- Full Text
- View/download PDF
36. Immunomodulatory activity of Toxicodendron pubescens in experimental models.
- Author
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Patil CR, Salunkhe PS, Gaushal MH, Gadekar AR, Agrawal AM, and Surana SJ
- Subjects
- Animals, Disease Models, Animal, Dose-Response Relationship, Drug, Immunity, Cellular drug effects, Mice, Mice, Inbred C57BL, Phytotherapy, Plant Extracts pharmacology, Anti-Inflammatory Agents, Non-Steroidal pharmacology, Homeopathy methods, Immunologic Factors pharmacology, Toxicodendron
- Abstract
Background: Toxicodendron pubescens is a botanical name of Rhus toxicodendron (Rhus tox). This plant is widely used in its homeopathically diluted form in the treatment of inflammatory and edematous conditions. In this study, various dilutions of Rhus tox including its crude form have been evaluated for their effects on immune response in the in vivo and in vitro experimental models., Methods: Rhus tox in the form of mother tincture, 6cH, 30cH, 200cH and 1000cH dilutions was tested through in vivo models including sheep red blood cells (SRBCs) induced cellular and humoral immune response in C57/BL6 mice. The effects of Rhus tox dilutions were also evaluated in vitro on the functions of human polymorphonuclear (PMN) cells such as phagocytosis and intracellular killing of Candida albicans, chemotaxis, and reduction of nitroblue tetrazolium (NBT) dye., Results: Rhus tox was found to intensify SRBCs induced antibody titer and delayed type hypersensitivity response in mice. Even higher dilutions such as 200cH and 1000cH were found to affect the immune response; however, the crude form, mother tincture, 6cH and 30cH dilutions revealed more potent effects than the 200cH and 1000cH dilutions. In in vitro assays, all the dilutions exerted stimulation of phagocytosis, candidacidal activity and chemotaxis of human PMN cells. The NBT dye reduction assay revealed that oxidative processes in the PMN cells are accelerated in the presence of Rhus tox. This study shows that Rhus tox possesses immunostimulatory activity in its crude form as well as in homeopathically diluted forms. These effects appeared to be concentration dependent as higher dilutions had less potent effects.
- Published
- 2009
- Full Text
- View/download PDF
37. Antioxidant and cytotoxic activities of Caesalpinia pulcherrima wood.
- Author
-
Pawar CR, Mutha RE, Landge AD, Jadhav RB, and Surana SJ
- Subjects
- Animals, Artemia drug effects, Humans, Caesalpinia chemistry, Free Radical Scavengers pharmacology, Free Radical Scavengers toxicity, Plant Extracts pharmacology, Plant Extracts toxicity, Wood chemistry
- Abstract
Antioxidant and cytotoxic activities of the methanolic and aqueous extracts of Caesalpinia pulcherrima wood were studied in in vitro models. Both extracts exhibited strong antioxidant activity, as evidenced by the low IC50 values in both 1,1-diphenyl-2-picryl hydrazyl (DPPH), nitric oxide and superoxide scavenging methods; the values were found to be less or comparable to those of gallic acid, the standard used. To determine the cytotoxic activity, extracts were tested for toxic effects to brine shrimp larvae. In this assay, the methanolic extract had little effect, but aqueous extract was relatively toxic. The antioxidant and cytotoxic activities may be attributed to the total phenolic content in the wood.
- Published
- 2009
38. Formulation and evaluation of oral mucoadhesive multiparticulate system containing metoprolol tartarate: an in vitro-ex vivo characterization.
- Author
-
Belgamwar V, Shah V, and Surana SJ
- Subjects
- Adhesiveness, Administration, Oral, Animals, Chemistry, Pharmaceutical, Drug Stability, Gels, Microspheres, Rats, Solubility, Spectroscopy, Fourier Transform Infrared, Adrenergic beta-Antagonists chemistry, Drug Delivery Systems, Metoprolol chemistry
- Abstract
The aim of the present study was to prepare mucoadhesive multiparticulate system for oral drug delivery using ionic gelation technique. Microspheres composed of various mucoadhesive polymers including HPMC of various grades like K4M, K15M, K100M, E50LV, Carbopol of grades 971P, 974P and polycarbophil were prepared. In this technique cross linking of sodium alginate with calcium chloride was done which retarded the release of drug from the mucoadhesive polymer. In the present work Metoprolol tararate was used as a model drug. Interaction studies performed using FTIR spectroscopy revealed that there was no drug to polymer interactions. The preliminary mucoadhesive strength studies performed for various polymers using rotating cylindrical method showed that HPMC had greater mucoadhesive properties than carbopol and polycarbophil. Microspheres so prepared were discrete, bulky, free flowing and showed an average encapsulation efficiency ranging from 50-60%. Particle size of the microspheres, as determined by the optical microscopy was found to be between 400-650 microm. The prepared formulations also exhibited a good mucoadhesive strength which was determined in in vitro conditions through falling film technique and was compared with ex vivo studies. The microspheres so prepared also exhibited a good swelling index which confirmed the strong mucoadhesive property of the formulation. Metoprolol release from the multiparticulate system was regulated and extended until 12 hours and exhibited a non fickian drug release kinetics approaching to zero order, as evident from the release rate exponent values which varied between 0.57 to 0.73. The stability studies performed on the optimized batches at 40 degrees C /75% RH for 90 days indicated no significant change in the physicochemical properties.
- Published
- 2009
- Full Text
- View/download PDF
39. Validated HPTLC method for simultaneous estimation of levofloxacin hemihydrate and ornidazole in pharmaceutical dosage form.
- Author
-
Chepurwar SB, Shirkhedkar AA, Bari SB, Fursule RA, and Surana SJ
- Subjects
- Calibration, Reference Standards, Reproducibility of Results, Sensitivity and Specificity, Amebicides analysis, Anti-Bacterial Agents analysis, Chromatography, Thin Layer methods, Dosage Forms, Levofloxacin, Ofloxacin analysis, Ornidazole analysis
- Abstract
A simple, rapid, and accurate high-performance thin-layer chromatography (HPTLC) method is described for the simultaneous determination of levofloxacin hemihydrate and ornidazole in tablet dosage form. The method is based on the HPTLC separation of the two drugs followed by densitometric measurements of their spots at 298 nm. The separation is carried out on Merck TLC aluminium sheets of silica gel 60 F254 using n-butanol-methanol-ammonia (5:1:1.5, v/v/v) as mobile phase. The linearity is found to be in the range of 50-250 and 100-500 ng/spot for levofloxacin hemihydrate and ornidazole, respectively. The method is successively applied to pharmaceutical formulation because no chromatographic interferences from the tablet excipients are found. The suitability of this HPTLC method for the quantitative determination of the compounds is proved by validation in accordance with the requirements laid down by International Conference on Harmonization (ICH) guidelines.
- Published
- 2007
- Full Text
- View/download PDF
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