Search

Your search keyword '"Shields, Kathleen"' showing total 116 results
Start Over Author "Shields, Kathleen" Language english
116 results on '"Shields, Kathleen"'

1. The San Antonio Sodium Reduction Initiative: Lessons Learned and Recommendations

Author

Sosa, Erica T., Ullevig, Sarah L., Spitsen, Ellen, Heine, Anne, Perez, Casey, and Shields, Kathleen

Abstract

Background: The Sodium Reduction Initiative (SRI), a voluntary collaboration among the local health department and community partners, aimed to reduce sodium in worksites and federally funded meal programs throughout Bexar County. Purpose: The purpose of this study was to assess sodium-related purchasing, preparation, and policies among food providers before and after the SRI and identify factors impacting implementation. Methods: Sodium-related practices and policies were assessed using the Sodium Practices Assessment Tool (SPAT). Stakeholder interviews were conducted to identify factors impacting initiative implementation. Results: SPAT data revealed increased healthy practices in worksites after SRI, including using recipes and measuring salt during food preparation. Qualitative analysis of interviews revealed that implementation was facilitated by the SRI team's expertise; existing health initiatives; and an organizational culture of wellness but impeded by concerns regarding customer dissatisfaction with lower sodium items; lower sodium ingredient availability and expense; and time constraints. Discussion: Organizations interested in implementing similar initiatives might consider embedding efforts into existing health initiatives; establishing organizational networks to share resources; and providing new partners with evaluation assistance. Translation to Health Education Practice: Health Educators can educate foodservice providers on the importance of sodium reduction as well as develop and evaluate sodium reduction initiatives.

Published
2019
Full Text
View/download PDF

2. Partial Loss of USP9X Function Leads to a Male Neurodevelopmental and Behavioral Disorder Converging on Transforming Growth Factor β Signaling

Author

Pena, Loren, Shashi, Vandana, Schoch, Kelly, Sullivan, Jennifer A., Acosta, Maria T., Adams, David R., Aday, Aaron, Alejandro, Mercedes E., Allard, Patrick, Ashley, Euan A., Azamian, Mahshid S., Bacino, Carlos A., Bademci, Guney, Baker, Eva, Balasubramanyam, Ashok, Baldridge, Dustin, Barbouth, Deborah, Batzli, Gabriel F., Beggs, Alan H., Bellen, Hugo J., Bernstein, Jonathan A., Berry, Gerard T., Bican, Anna, Bick, David P., Birch, Camille L., Bivona, Stephanie, Bonnenmann, Carsten, Bonner, Devon, Boone, Braden E., Bostwick, Bret L., Briere, Lauren C., Brokamp, Elly, Brown, Donna M., Brush, Matthew, Burke, Elizabeth A., Burrage, Lindsay C., Butte, Manish J., Carrasquillo, Olveen, Peter Chang, Ta Chen, Chao, Hsiao-Tuan, Clark, Gary D., Coakley, Terra R., Cobban, Laurel A., Cogan, Joy D., Cole, F. Sessions, Colley, Heather A., Cooper, Cynthia M., Cope, Heidi, Craigen, William J., D'Souza, Precilla, Dasari, Surendra, Davids, Mariska, Davidson, Jean M., Dayal, Jyoti G., Dell'Angelica, Esteban C., Dhar, Shweta U., Dorrani, Naghmeh, Dorset, Daniel C., Douine, Emilie D., Draper, David D., Dries, Annika M., Duncan, Laura, Eckstein, David J., Emrick, Lisa T., Eng, Christine M., Enns, Gregory M., Esteves, Cecilia, Estwick, Tyra, Fernandez, Liliana, Ferreira, Carlos, Fieg, Elizabeth L., Fisher, Paul G., Fogel, Brent L., Forghani, Irman, Friedman, Noah D., Gahl, William A., Godfrey, Rena A., Goldman, Alica M., Goldstein, David B., Gourdine, Jean-Philippe F., Grajewski, Alana, Groden, Catherine A., Gropman, Andrea L., Haendel, Melissa, Hamid, Rizwan, Hanchard, Neil A., High, Frances, Holm, Ingrid A., Hom, Jason, Huang, Alden, Huang, Yong, Isasi, Rosario, Jamal, Fariha, Jiang, Yong-hui, Johnston, Jean M., Jones, Angela L., Karaviti, Lefkothea, Kelley, Emily G., Koeller, David M., Kohane, Isaac S., Kohler, Jennefer N., Krakow, Deborah, Krasnewich, Donna M., Korrick, Susan, Koziura, Mary, Krier, Joel B., Kyle, Jennifer E., Lalani, Seema R., Lam, Byron, Lanpher, Brendan C., Lanza, Ian R., Lau, C. Christopher, Lazar, Jozef, LeBlanc, Kimberly, Lee, Brendan H., Lee, Hane, Levitt, Roy, Levy, Shawn E., Lewis, Richard A., Lincoln, Sharyn A., Liu, Pengfei, Liu, Xue Zhong, Loo, Sandra K., Loscalzo, Joseph, Maas, Richard L., Macnamara, Ellen F., MacRae, Calum A., Maduro, Valerie V., Majcherska, Marta M., Malicdan, May Christine V., Mamounas, Laura A., Manolio, Teri A., Markello, Thomas C., Marom, Ronit, Martin, Martin G., Martínez-Agosto, Julian A., Marwaha, Shruti, May, Thomas, McCauley, Jacob, McConkie-Rosell, Allyn, McCormack, Colleen E., McCray, Alexa T., Merker, Jason D., Metz, Thomas O., Might, Matthew, Morava-Kozicz, Eva, Moretti, Paolo M., Morimoto, Marie, Mulvihill, John J., Murdock, David R., Nath, Avi, Nelson, Stan F., Newberry, J. Scott, Newman, John H., Nicholas, Sarah K., Novacic, Donna, Oglesbee, Devin, Orengo, James P., Pak, Stephen, Pallais, J. Carl, Palmer, Christina GS., Papp, Jeanette C., Parker, Neil H., Phillips, John A., III, Posey, Jennifer E., Postlethwait, John H., Potocki, Lorraine, Pusey, Barbara N., Renteri, Genecee, Reuter, Chloe M., Rives, Lynette, Robertson, Amy K., Rodan, Lance H., Rosenfeld, Jill A., Rowley, Robb K., Sacco, Ralph, Sampson, Jacinda B., Samson, Susan L., Saporta, Mario, Schaechter, Judy, Schedl, Timothy, Scott, Daryl A., Shakachite, Lisa, Sharma, Prashant, Shields, Kathleen, Shin, Jimann, Signer, Rebecca, Sillari, Catherine H., Silverman, Edwin K., Sinsheimer, Janet S., Smith, Kevin S., Solnica-Krezel, Lilianna, Spillmann, Rebecca C., Stoler, Joan M., Stong, Nicholas, Sweetser, David A., Tamburro, Cecelia P., Tan, Queenie K.-G., Tekin, Mustafa, Telischi, Fred, Thorson, Willa, Tifft, Cynthia J., Toro, Camilo, Tran, Alyssa A., Urv, Tiina K., Vogel, Tiphanie P., Waggott, Daryl M., Wahl, Colleen E., Walley, Nicole M., Walsh, Chris A., Walker, Melissa, Wambach, Jennifer, Wan, Jijun, Wang, Lee-kai, Wangler, Michael F., Ward, Patricia A., Waters, Katrina M., Webb-Robertson, Bobbie-Jo M., Wegner, Daniel, Westerfield, Monte, Wheeler, Matthew T., Wise, Anastasia L., Wolfe, Lynne A., Woods, Jeremy D., Worthey, Elizabeth A., Yamamoto, Shinya, Yang, John, Yoon, Amanda J., Yu, Guoyun, Zastrow, Diane B., Zhao, Chunli, Zuchner, Stephan, Gahl, William, Johnson, Brett V., Kumar, Raman, Oishi, Sabrina, Alexander, Suzy, Kasherman, Maria, Vega, Michelle Sanchez, Ivancevic, Atma, Gardner, Alison, Domingo, Deepti, Corbett, Mark, Parnell, Euan, Yoon, Sehyoun, Oh, Tracey, Lines, Matthew, Lefroy, Henrietta, Kini, Usha, Van Allen, Margot, Grønborg, Sabine, Mercier, Sandra, Küry, Sébastien, Bézieau, Stéphane, Pasquier, Laurent, Raynaud, Martine, Afenjar, Alexandra, Billette de Villemeur, Thierry, Keren, Boris, Désir, Julie, Van Maldergem, Lionel, Marangoni, Martina, Dikow, Nicola, Koolen, David A., VanHasselt, Peter M., Weiss, Marjan, Zwijnenburg, Petra, Sa, Joaquim, Reis, Claudia Falcao, López-Otín, Carlos, Santiago-Fernández, Olaya, Fernández-Jaén, Alberto, Rauch, Anita, Steindl, Katharina, Joset, Pascal, Goldstein, Amy, Madan-Khetarpal, Suneeta, Infante, Elena, Zackai, Elaine, Mcdougall, Carey, Narayanan, Vinodh, Ramsey, Keri, Mercimek-Andrews, Saadet, Pinto e Vairo, Filippo, Pichurin, Pavel N., Ewing, Sarah A., Barnett, Sarah S., Klee, Eric W., Perry, M. Scott, Koenig, Mary Kay, Keegan, Catherine E., Schuette, Jane L., Asher, Stephanie, Perilla-Young, Yezmin, Smith, Laurie D., Bhoj, Elizabeth, Kaplan, Paige, Li, Dong, Oegema, Renske, van Binsbergen, Ellen, van der Zwaag, Bert, Smeland, Marie Falkenberg, Cutcutache, Ioana, Page, Matthew, Armstrong, Martin, Lin, Angela E., Steeves, Marcie A., Hollander, Nicolette den, Hoffer, Mariëtte J.V., Reijnders, Margot R.F., Demirdas, Serwet, Koboldt, Daniel C., Bartholomew, Dennis, Mosher, Theresa Mihalic, Hickey, Scott E., Shieh, Christine, Sanchez-Lara, Pedro A., Graham, John M., Jr., Tezcan, Kamer, Schaefer, G.B., Danylchuk, Noelle R., Asamoah, Alexander, Jackson, Kelly E., Yachelevich, Naomi, Au, Margaret, Pérez-Jurado, Luis A., Kleefstra, Tjitske, Penzes, Peter, Wood, Stephen A., Burne, Thomas, Pierson, Tyler Mark, Piper, Michael, Gécz, Jozef, and Jolly, Lachlan A.

Published
2020
Full Text
View/download PDF

6. Comptes rendus

Author

Shields, Kathleen, Draga Alexandru, Maria-Sabina, Zupančič, Metka, Le Juez, Brigitte, and Ivana, Dragoș

Published
2023
Full Text
View/download PDF

10. Translation and Conflict

Author

Shields, Kathleen

Subjects
Conflit, Self-translation, Memoir, Conflict, Auto-traduction, Free translation, Jean Follain, Mémoire, Ciaran Carson, Traduction libre
Abstract

Dans son recueil From Elsewhere (2014), Ciaran Carson juxtapose une traduction de Jean Follain et, en regard, une deuxième traduction de cette première version. Des oppositions tensionnelles en résultent : traduction fidèle et libre ; traduction et auto-traduction ; les perspectives de celui qui enregistre et celui qui témoigne. Ce processus ouvert de la traduction facilite l’écriture de mémoires où les évènements violents en Irlande du Nord peuvent être confrontés., In Ciaran Carson’s translation experiment From Elsewhere (2014), translations of Jean Follain are set beside second free translations of the first version throughout. The ensuing contrasts are held in tension: between close and free translation; translation and self-translation; the two historical perspectives of recorder and witness. In this open-ended translation process, extended personal memoir writing can confront the violent events of the Troubles in Northern Ireland many years later.

Published
2022
Full Text
View/download PDF

12. Genetics in the NICU: Nurses' Perceived Knowledge and Desired Education.

Author

Shields, Kathleen, Czerwinski, Jennifer, Dauwe, Tracie, Hashmi, Syed, Hillman, Paul, Mowrey, Kate, and Gunther, Kathryn

Subjects
NEONATAL intensive care, NURSING, NURSES' attitudes, HUMAN comfort, CROSS-sectional method, NEONATAL intensive care units, MANN Whitney U Test, MEDICAL genetics, DESCRIPTIVE statistics, CLINICAL competence, DATA analysis software, CONTENT analysis
Abstract

Background: Many infants admitted to the neonatal intensive care unit (NICU) have genetic conditions. Previous research has shown that gaps exist in the genetics knowledge of nurses and that they lack comfort applying genetics information to clinical practice. Studies assessing the knowledge or comfort of NICU nurses with genetics have not previously been completed. Method: A total of 122 NICU nurses completed a survey assessing perceived knowledge of genetics, comfort with clinical scenarios involving genetics, and desired genetics education. Results: Perceived knowledge and overall comfort were correlated with highest degree received, how prepared a nurse felt by the genetics education received in their training, and having a close relationship with someone with a genetic condition. Almost all respondents (96%, n = 117) desired additional genetics education. Conclusion: Gaps exist in the genetics knowledge of neonatal nurses in our cohort, and their overall comfort working with clinical scenarios involving genetics was low. There is significant interest in additional genetics education. [J Contin Educ Nurs. 2023;54(1):16–24.] [ABSTRACT FROM AUTHOR]

Published
2023
Full Text
View/download PDF

13. Thomas MacGreevy

Author

Shields, Kathleen

Subjects
Literature/writing
Abstract

Magnanimity is a good quality in a biographer. Elaine Showalter (June 25) repeats for comic effect Deirdre Bair's rather unkind sketch of Thomas MacGreevy's nieces' hospitality. While researching the work [...]

Published
2021

17. Strategic Sodium Reduction Initiative Reduces Sodium in Meals Served at Older Adult Congregate Meal Program Sites.

Author

Ullevig, Sarah L., Spitsen, Ellen, Heine, Anne C., Balarin, Ashton, Uc, Eliani, Shields, Kathleen, and Sosa, Erica T.

Subjects
KRUSKAL-Wallis Test, SALT-free diet, ANALYSIS of variance, HEALTH outcome assessment, PUBLIC health, NUTRITIONAL requirements, HUMAN services programs, SURVEYS, FOOD, CHI-squared test, MENU planning, FOOD service, CUSTOMER satisfaction
Abstract

Excessive sodium consumption is a public health issue and congregate meal programs provide a unique opportunity to reduce sodium served to a large, at-risk population. A Sodium Reduction Initiative (SRI) was implemented in a congregate meal program that serves over 3,000 older adults. Nutrient analyses conducted at baseline and post-intervention were used to calculate average sodium reduction and the number of low sodium foods; targeted foods were categorized by strategy. Customer satisfaction surveys were collected at baseline and 3- and 6-months post-intervention. Kruskal Wallis and analysis of variance were used to compare sodium reduction differences. Chi-square analysis determined associations among strategies. The SRI impacted 55 foods, low sodium foods increased by 22%, and the average sodium per menu cycle was reduced by 21%. Replacement with a lower sodium food was the most frequently used strategy and had the largest sodium reduction. Sauces and main entrees were most frequently impacted, and thirteen ingredients accounted for 75% of all reduced-sodium foods. Over 50% of the 1,424 survey respondents consumed the reduced-sodium foods and food satisfaction remained stable from baseline to post-intervention. Congregate meals programs that target commonly used foods and key ingredients can significantly reduce sodium served to older adults. [ABSTRACT FROM AUTHOR]

Published
2022
Full Text
View/download PDF

20. De Novo Variants in WDR37 Are Associated with Epilepsy, Colobomas, Dysmorphism, Developmental Delay, Intellectual Disability, and Cerebellar Hypoplasia

Author

Acosta, Maria T., Adams, David R., Agrawal, Pankaj, Alejandro, Mercedes E., Allard, Patrick, Alvey, Justin, Andrews, Ashley, Ashley, Euan A., Azamian, Mahshid S., Bacino, Carlos A., Bademci, Guney, Baker, Eva, Balasubramanyam, Ashok, Baldridge, Dustin, Bale, Jim, Barbouth, Deborah, Batzli, Gabriel F., Bayrak-Toydemir, Pinar, Beggs, Alan H., Bejerano, Gill, Bellen, Hugo J., Bernstein, Jonathan A., Berry, Gerard T., Bican, Anna, Bick, David P., Birch, Camille L., Bivona, Stephanie, Bohnsack, John, Bonnenmann, Carsten, Bonner, Devon, Boone, Braden E., Bostwick, Bret L., Botto, Lorenzo, Briere, Lauren C., Brokamp, Elly, Brown, Donna M., Brush, Matthew, Burke, Elizabeth A., Burrage, Lindsay C., Butte, Manish J., Carey, John, Carrasquillo, Olveen, Chang, Ta Chen Peter, Chao, Hsiao-Tuan, Clark, Gary D., Coakley, Terra R., Cobban, Laurel A., Cogan, Joy D., Cole, F. Sessions, Colley, Heather A., Cooper, Cynthia M., Cope, Heidi, Craigen, William J., D'Souza, Precilla, Dasari, Surendra, Davids, Mariska, Dayal, Jyoti G., Dell'Angelica, Esteban C., Dhar, Shweta U., Dorrani, Naghmeh, Dorset, Daniel C., Douine, Emilie D., Draper, David D., Duncan, Laura, Eckstein, David J., Emrick, Lisa T., Eng, Christine M., Esteves, Cecilia, Estwick, Tyra, Fernandez, Liliana, Ferreira, Carlos, Fieg, Elizabeth L., Fisher, Paul G., Fogel, Brent L., Forghani, Irman, Fresard, Laure, Gahl, William A., Godfrey, Rena A., Goldman, Alica M., Goldstein, David B., Gourdine, Jean-Philippe F., Grajewski, Alana, Groden, Catherine A., Gropman, Andrea L., Haendel, Melissa, Hamid, Rizwan, Hanchard, Neil A., Hayes, Nichole, High, Frances, Holm, Ingrid A., Hom, Jason, Huang, Alden, Huang, Yong, Isasi, Rosario, Jamal, Fariha, Jiang, Yong-hui, Johnston, Jean M., Jones, Angela L., Karaviti, Lefkothea, Kelley, Emily G., Kiley, Dana, Koeller, David M., Kohane, Isaac S., Kohler, Jennefer N., Krakow, Deborah, Krasnewich, Donna M., Korrick, Susan, Koziura, Mary, Krier, Joel B., Kyle, Jennifer E., Lalani, Seema R., Lam, Byron, Lanpher, Brendan C., Lanza, Ian R., Lau, C. Christopher, Lazar, Jozef, LeBlanc, Kimberly, Lee, Brendan H., Lee, Hane, Levitt, Roy, Levy, Shawn E., Lewis, Richard A., Lincoln, Sharyn A., Liu, Pengfei, Liu, Xue Zhong, Longo, Nicola, Loo, Sandra K., Loscalzo, Joseph, Maas, Richard L., Macnamara, Ellen F., MacRae, Calum A., Maduro, Valerie V., Majcherska, Marta M., Malicdan, May Christine V., Mamounas, Laura A., Manolio, Teri A., Mao, Rong, Markello, Thomas C., Marom, Ronit, Marth, Gabor, Martin, Beth A., Martin, Martin G., Martínez-Agosto, Julian A., Marwaha, Shruti, May, Thomas, McCauley, Jacob, McConkie-Rosell, Allyn, McCormack, Colleen E., McCray, Alexa T., Metz, Thomas O., Might, Matthew, Morava-Kozicz, Eva, Moretti, Paolo M., Morimoto, Marie, Mulvihill, John J., Murdock, David R., Nath, Avi, Nelson, Stan F., Newberry, J. Scott, Newman, John H., Nicholas, Sarah K., Novacic, Donna, Oglesbee, Devin, Orengo, James P., Pace, Laura, Pak, Stephen, Pallais, J. Carl, Palmer, Christina G.S., Papp, Jeanette C., Parker, Neil H., Phillips, John A., III, Posey, Jennifer E., Postlethwait, John H., Potocki, Lorraine, Pusey, Barbara N., Quinlan, Aaron, Raja, Archana N., Renteria, Genecee, Reuter, Chloe M., Rives, Lynette, Robertson, Amy K., Rodan, Lance H., Rosenfeld, Jill A., Rowley, Robb K., Ruzhnikov, Maura, Sacco, Ralph, Sampson, Jacinda B., Samson, Susan L., Saporta, Mario, Schaechter, Judy, Schedl, Timothy, Schoch, Kelly, Scott, Daryl A., Shakachite, Lisa, Sharma, Prashant, Shashi, Vandana, Shields, Kathleen, Shin, Jimann, Signer, Rebecca, Sillari, Catherine H., Silverman, Edwin K., Sinsheimer, Janet S., Sisco, Kathy, Smith, Kevin S., Solnica-Krezel, Lilianna, Spillmann, Rebecca C., Stoler, Joan M., Stong, Nicholas, Sullivan, Jennifer A., Sutton, Shirley, Sweetser, David A., Tabor, Holly K., Tamburro, Cecelia P., Tan, Queenie K.-G., Tekin, Mustafa, Telischi, Fred, Thorson, Willa, Tifft, Cynthia J., Toro, Camilo, Tran, Alyssa A., Urv, Tiina K., Velinder, Matt, Viskochil, Dave, Vogel, Tiphanie P., Wahl, Colleen E., Walley, Nicole M., Walsh, Chris A., Walker, Melissa, Wambach, Jennifer, Wan, Jijun, Wang, Lee-kai, Wangler, Michael F., Ward, Patricia A., Waters, Katrina M., Webb-Robertson, Bobbie-Jo M., Wegner, Daniel, Westerfield, Monte, Wheeler, Matthew T., Wise, Anastasia L., Wolfe, Lynne A., Woods, Jeremy D., Worthey, Elizabeth A., Yamamoto, Shinya, Yang, John, Yoon, Amanda J., Yu, Guoyun, Zastrow, Diane B., Zhao, Chunli, Zuchner, Stephan, Kanca, Oguz, Andrews, Jonathan C., Lee, Pei-Tseng, Patel, Chirag, Braddock, Stephen R., Slavotinek, Anne M., Cohen, Julie S., Gubbels, Cynthia S., Aldinger, Kimberly A., Williams, Judy, Indaram, Maanasa, Fatemi, Ali, Yu, Timothy W., Agrawal, Pankaj B., Vezina, Gilbert, Simons, Cas, Crawford, Joanna, Chung, Wendy K., and Dobyns, William B.

Published
2019
Full Text
View/download PDF

23. TREM-1 expression is increased in the synovium of rheumatoid arthritis patients and induces the expression of pro-inflammatory cytokines

Author

Kuai, Jun, Gregory, Bernard, Hill, Andrew, Pittman, Debra D., Feldman, Jeffrey L., Brown, Tom, Carito, Brenda, O’Toole, Margot, Ramsey, Renee, Adolfsson, Oskar, Shields, Kathleen M., Dower, Ken, Hall, J Perry, Kurdi, Yahya, Beech, Jonathan T., Nanchahal, Jagdeep, Feldmann, Marc, Foxwell, Brian M., Brennan, Fionula M., Winkler, David G., and Lin, Lih-Ling

Published
2009

29. Behavioral and cognitive functioning in individuals with Cantú syndrome.

Author

Roessler, Helen I., Heuvel, Lieke M., Shields, Kathleen, Guilliams, Kristin P., Knoers, Nine V. A. M., Haaften, Gijs, Grange, Dorothy K., and Haelst, Mieke M.

Abstract

Cantú syndrome (CS) is caused by pathogenic variants in ABCC9 and KCNJ8 encoding the regulatory and pore‐forming subunits of ATP‐sensitive potassium (KATP) channels. CS is characterized by congenital hypertrichosis, distinctive facial features, peripheral edema, and cardiac and neurodevelopmental abnormalities. Behavioral and cognitive issues have been self‐reported by some CS individuals, but results of formal standardized investigations have not been published. To assess the cognitive profile, social functioning, and psychiatric symptoms in a large group of CS subjects systematically in a cross‐sectional manner, we invited 35 individuals (1–69 years) with confirmed ABCC9 variants and their relatives to complete various commonly applied standardized age‐related questionnaires, including the Kaufman brief intelligence test 2, the social responsiveness scale‐2, and the Achenbach system of empirically based assessment. The majority of CS individuals demonstrated average verbal and nonverbal intelligence compared to the general population. Fifteen percent of cases showed social functioning strongly associated with a clinical diagnosis of autism spectrum disorder. Both externalizing and internalizing problems were also present in this cohort. In particular, anxiety, anxiety or attention deficit hyperactivity disorder, and autism spectrum behaviors were predominantly observed in the younger subjects in the cohort (≥25%), but this percentage decreased markedly in adults. [ABSTRACT FROM AUTHOR]

Published
2021
Full Text
View/download PDF

30. Genetic rearrangement during site specific integration event facilitates cell line development of a bispecific molecule.

Author

Tevelev, Barbara, Patel, Himakshi, Shields, Kathleen, Wei, Wei, Cooley, Cecilia, Zhang, Sam, Bitzas, Gabrielle, Duan, Weili, Khetemenee, Lam, Jackobek, Ryan, D'Antona, Aaron, Sievers, Annette, King, Amy, Tam, Amy, Zhang, Yan, Sousa, Eric, Cohen, Justin, Wroblewska, Lila, Marshall, Jeffrey, and Jackson, Martha

Subjects
CELL lines, PHENOTYPES, MOLECULES, GENOTYPES, MONOCLONAL antibodies, BISPECIFIC antibodies, GENE delivery techniques
Abstract

Site specific integration (SSI) expression systems offer robust means of generating highly productive and stable cell lines for traditional monoclonal antibodies. As complex modalities such as antibody‐like molecules comprised of greater than two peptides become more prevalent, greater emphasis needs to be placed on the ability to produce appreciable quantities of the correct product of interest (POI). The ability to screen several transcript stoichiometries could play a large role in ensuring high amounts of the correct POI. Here we illustrate implementation of an SSI expression system with a single site of integration for development and production of a multi‐chain, bi‐specific molecule. A SSI vector with a single copy of all of the genes of interest was initially selected for stable Chinese hamster ovary transfection. While the resulting transfection pools generated low levels of the desired heterodimer, utilizing an intensive clone screen strategy, we were able to identify clones having significantly higher levels of POI. In‐depth genotypic characterization of clones having the desirable phenotype revealed that a duplication of the light chain within the landing pad was responsible for producing the intended molecule. Retrospective transfection pool analysis using a vector configuration mimicking the transgene configuration found in the clones, as well as other vector configurations, yielded more favorable results with respect to % POI. Overall, the study demonstrated that despite the theoretical static nature of the SSI expression system, enough heterogeneity existed to yield clones having significantly different transgene phenotypes/genotypes and support production of a complex multi‐chain molecule. [ABSTRACT FROM AUTHOR]

Published
2021
Full Text
View/download PDF

31. One is the loneliest number: genotypic matchmaking using the electronic health record

Author

Brokamp, Elly, Koziura, Mary E., Phillips, John A., III, Tang, Leigh Anne, Cogan, Joy D., Rives, Lynette C., Robertson, Amy K., Duncan, Laura, Bican, Anna, Peterson, Josh F., Newman, John H., Hamid, Rizwan, Bastarache, Lisa, Alejandro, Mercedes E., Azamian, Mahshid S., Bacino, Carlos A., Balasubramanyam, Ashok, Bostwick, Bret L., Burrage, Lindsay C., Chao, Hsiao-Tuan, Clark, Gary D., Craigen, William J., Dhar, Shweta U., Emrick, Lisa T., Goldman, Alica M., Hanchard, Neil A., Jamal, Fariha, Karaviti, Lefkothea, Lalani, Seema R., Lee, Brendan H., Lewis, Richard A., Marom, Ronit, Moretti, Paolo M., Murdock, David R., Nicholas, Sarah K., Orengo, James P., Posey, Jennifer E., Potocki, Lorraine, Rosenfeld, Jill A., Samson, Susan L., Scott, Daryl A., Tran, Alyssa A., Vogel, Tiphanie P., Bellen, Hugo J., Wangler, Michael F., Yamamoto, Shinya, Eng, Christine M., Liu, Pengfei, Ward, Patricia A., Goldstein, David B., Stong, Nicholas, Cope, Heidi, Jiang, Yong-hui, McConkie-Rosell, Allyn, Schoch, Kelly, Shashi, Vandana, Spillmann, Rebecca C., Sullivan, Jennifer A., Tan, Queenie K.-G., Walley, Nicole M., Agrawal, Pankaj, Beggs, Alan H., Berry, Gerard T., Briere, Lauren C., Cooper, Cynthia M., Cobban, Laurel A., Fieg, Elizabeth L., High, Frances, Holm, Ingrid A., Korrick, Susan, Krier, Joel B., Lincoln, Sharyn A., Loscalzo, Joseph, Maas, Richard L., MacRae, Calum A., Pallais, J. Carl, Rodan, Lance H., Silverman, Edwin K., Stoler, Joan M., Sweetser, David A., Walker, Melissa, Walsh, Chris A., Esteves, Cecilia, Kelley, Emily G., Kohane, Isaac S., LeBlanc, Kimberly, McCray, Alexa T., Might, Matthew, Bick, David P., Birch, Camille L., Boone, Braden E., Brown, Donna M., Dorset, Daniel C., Jones, Angela L., Lazar, Jozef, Levy, Shawn E., May, Thomas, Newberry, J. Scott, Worthey, Elizabeth A., Lanza, Ian R., Oglesbee, Devin, Morava-Kozicz, Eva, Lanpher, Brendan C., Dasari, Surendra, Bademci, Guney, Barbouth, Deborah, Bivona, Stephanie, Carrasquillo, Olveen, Chang, Ta Chen Peter, Forghani, Irman, Grajewski, Alana, Isasi, Rosario, Lam, Byron, Levitt, Roy, Liu, Xue Zhong, McCauley, Jacob, Sacco, Ralph, Saporta, Mario, Schaechter, Judy, Tekin, Mustafa, Telischi, Fred, Thorson, Willa, Zuchner, Stephan, Batzli, Gabriel F., Colley, Heather A., Dayal, Jyoti G., Eckstein, David J., Krasnewich, Donna M., Mamounas, Laura A., Manolio, Teri A., Mulvihill, John J., Rowley, Robb K., Sillari, Catherine H., Tamburro, Cecelia P., Urv, Tiina K., Wise, Anastasia L., Acosta, Maria T., Bonnenmann, Carsten, Burke, Elizabeth A., D’Souza, Precilla, Davids, Mariska, Draper, David D., Estwick, Tyra, Ferreira, Carlos, Godfrey, Rena A., Groden, Catherine A., Johnston, Jean M., Lau, C. Christopher, Macnamara, Ellen F., Maduro, Valerie V., Markello, Thomas C., Morimoto, Marie, Nath, Avi, Novacic, Donna, Pusey, Barbara N., Sharma, Prashant, Toro, Camilo, Wahl, Colleen E., Yu, Guoyun, Gropman, Andrea L., Baker, Eva, Adams, David R., Gahl, William A., Malicdan, May Christine V., Tifft, Cynthia J., Wolfe, Lynne A., Yang, John, Brush, Matthew, Gourdine, Jean-Philippe F., Haendel, Melissa, Koeller, David M., Kyle, Jennifer E., Metz, Thomas O., Waters, Katrina M., Webb-Robertson, Bobbie-Jo M., Ashley, Euan A., Bernstein, Jonathan A., Bonner, Devon, Coakley, Terra R., Fernandez, Liliana, Fisher, Paul G., Hom, Jason, Huang, Yong, Kohler, Jennefer N., Majcherska, Marta M., Marwaha, Shruti, McCormack, Colleen E., Reuter, Chloe M., Sampson, Jacinda B., Smith, Kevin S., Wheeler, Matthew T., Zastrow, Diane B., Zhao, Chunli, Ruzhnikov, Maura, Fresard, Laure, Tabor, Holly K., Raja, Archana N., Bejerano, Gill, Martin, Beth A., Sutton, Shirley, Allard, Patrick, Butte, Manish J., Dell’Angelica, Esteban C., Dorrani, Naghmeh, Douine, Emilie D., Fogel, Brent L., Huang, Alden, Krakow, Deborah, Lee, Hane, Loo, Sandra K., Martin, Martin G., Martínez-Agosto, Julian A., Nelson, Stanley F., Palmer, Christina G.S., Papp, Jeanette C., Parker, Neil H., Renteria, Genecee, Signer, Rebecca H., Sinsheimer, Janet S., Wan, Jijun, Wang, Lee-kai, Woods, Jeremy D., Yoon, Amanda J., Botto, Lorenzo, Andrews, Ashley, Longo, Nicola, Carey, John, Bohnsack, John, Viskochil, Dave, Paolo, Moretti, Laura, Pace, Alvey, Justin, Bale, Jim, Marth, Gabor, Velinder, Matt, Quinlan, Aaron, Mao, Rong, Bayrak-Toydemir, Pinar, Postlethwait, John H., Westerfield, Monte, Shakachite, Lisa, Cole, F. Sessions, Hayes, Nichole, Kiley, Dana, Shields, Kathleen, Sisco, Kathy, Wambach, Jennifer, Wegner, Daniel, Baldridge, Dustin, Schedl, Timothy, Solnica-Krezel, Lilianna, Pak, Stephen, and Shin, Jimann

Published
2021
Full Text
View/download PDF

33. Three‐dimensional facial morphology in Cantú syndrome.

Author

Roessler, Helen I., Shields, Kathleen, Grange, Dorothy K., Knoers, Nine V.A.M., Haaften, Gijs, Hammond, Peter, and Haelst, Mieke M.

Abstract

Cantú syndrome (CS) was first described in 1982, and is caused by pathogenic variants in ABCC9 and KCNJ8 encoding regulatory and pore forming subunits of ATP‐sensitive potassium (KATP) channels, respectively. It is characterized by congenital hypertrichosis, osteochondrodysplasia, extensive cardiovascular abnormalities and distinctive facial anomalies including a broad nasal bridge, long philtrum, epicanthal folds, and prominent lips. Many genetic syndromes, such as CS, involve facial anomalies that serve as a significant clue in the initial identification of the respective disorder before clinical or molecular diagnosis are undertaken. However, an overwhelming number of CS patients receive misdiagnoses based on an evaluation of coarse facial features. By analyzing three‐dimensional images of CS faces, we quantified facial dysmorphology in a cohort of both male and female CS patients with confirmed ABCC9 variants. Morphometric analysis of different regions of the face revealed gender‐specific significant differences in face shape. Moreover, we show that 3D facial photographs can distinguish between CS and other genetic disorders with specific facial dysmorphologies that have been mistaken for CS‐associated anomalies in the past, hence assisting in an earlier clinical and molecular diagnosis. This optimizes genetic counseling and reduces stress for patients and parents by avoiding unnecessary misdiagnosis. [ABSTRACT FROM AUTHOR]

Published
2020
Full Text
View/download PDF

35. Identity theft: lessons from the TJX case.

Author

Shields, Kathleen Burden

Subjects
Identity theft -- Laws, regulations and rules, Credit cards -- Laws, regulations and rules, Class actions (Civil procedure) -- Laws, regulations and rules, Government regulation
Published
2007

39. Correction of the coagulation defect in hemophilia using a factor Xa variant with novel engineered protease function

Author

Ivanciu, Lacramioara, Toso, Raffaella, Margaritis, Paris, Pavani, Giulia, Kim, Haein, Schlachterman, Alexander, Liu, Jian-Hua, Clerin, Valerie, Pittman, Debra D., Rose-Miranda, Rosalind, Shields, Kathleen M., Erbe, David V., Tobin, James F., Arruda, Valder R., and Camire, Rodney M.

Subjects
Enzyme Precursors, Hemostasis, Mice, Inbred BALB C, Thrombin, Gene Expression, Hemorrhage, Factor VIIa, Hemophilia A, Article, Hemostatics, Recombinant Proteins, Thrombelastography, Mice, Inbred C57BL, Disease Models, Animal, Mice, HEK293 Cells, Factor Xa, Animals, Humans, Blood Coagulation
Abstract

Effective therapies are needed to control excessive bleeding in a range of clinical conditions. We describe a surprisingly useful approach to improve hemostasis in vivo using a variant of coagulation factor Xa (FXaI16L). This conformationally pliant derivative is partially inactive due to a defect in transitioning from zymogen to protease 1,2. Using mouse models of hemophilia, we show that FXaI16L has a prolonged half-life, relative to wild-type FXa and does not cause excessive activation of coagulation. Once clotting mechanisms are activated to produce its cofactor FVa, FXaI16L is driven to the protease state and restores hemostasis in hemophilic animals upon vascular injury. Moreover, using human or murine analogs, we show that FXaI16L is more efficacious than FVIIa which is used to treat bleeding in hemophilia inhibitor patients3. Because of its underlying mechanism of action, FXaI16L may provide an effective strategy to enhance blood clot formation and act as a rapid pan-hemostatic agent for the treatment of bleeding conditions.

Published
2011

40. Performance Standards for Restaurants : A New Approach to Addressing the Obesity Epidemic

Author

Cohen, Deborah, Bhatia, Rajiv, Story, Mary T., Sugarman, Stephen D., Wootan, Margo, Economos, Christina D., Van Horn, Linda, Whitsel, Laurie P., Roberts, Susan, Powell, Lisa M., Odoms-Young, Angela, Williams, Jerome D., Elbel, Brian, Harris, Jennifer, Kappagoda, Manel, Champagne, Catherine M., Shields, Kathleen, Lesser, Lenard I., Fox, Tracy, Becker, Nancy, Cohen, Deborah, Bhatia, Rajiv, Story, Mary T., Sugarman, Stephen D., Wootan, Margo, Economos, Christina D., Van Horn, Linda, Whitsel, Laurie P., Roberts, Susan, Powell, Lisa M., Odoms-Young, Angela, Williams, Jerome D., Elbel, Brian, Harris, Jennifer, Kappagoda, Manel, Champagne, Catherine M., Shields, Kathleen, Lesser, Lenard I., Fox, Tracy, and Becker, Nancy

Published
2013

41. Magnetic Resonance Imaging characteristics in case of TOR1AIP1 muscular dystrophy

Author

Acosta, Maria T., Adams, David R., Agrawal, Pankaj, Alejandro, Mercedes E., Allard, Patrick, Alvey, Justin, Andrews, Ashley, Ashley, Euan A., Azamian, Mahshid S., Bacino, Carlos A., Bademci, Guney, Baker, Eva, Balasubramanyam, Ashok, Baldridge, Dustin, Bale, Jim, Barbouth, Deborah, Batzli, Gabriel F., Bayrak-Toydemir, Pinar, Beggs, Alan H., Bejerano, Gill, Bellen, Hugo J., Bernstein, Jonathan A., Berry, Gerard T., Bican, Anna, Bick, David P., Birch, Camille L., Bivona, Stephanie, Bohnsack, John, Bonnenmann, Carsten, Bonner, Devon, Boone, Braden E., Bostwick, Bret L., Botto, Lorenzo, Briere, Lauren C., Brokamp, Elly, Brown, Donna M., Brush, Matthew, Burke, Elizabeth A., Burrage, Lindsay C., Butte, Manish J., Carey, John, Carrasquillo, Olveen, Chang, Ta Chen Peter, Chao, Hsiao-Tuan, Clark, Gary D., Coakley, Terra R., Cobban, Laurel A., Cogan, Joy D., Cole, F. Sessions, Colley, Heather A., Cooper, Cynthia M., Cope, Heidi, Craigen, William J., D'Souza, Precilla, Dasari, Surendra, Davids, Mariska, Dayal, Jyoti G., Dell'Angelica, Esteban C., Dhar, Shweta U., Dorrani, Naghmeh, Dorset, Daniel C., Douine, Emilie D., Draper, David D., Duncan, Laura, Eckstein, David J., Emrick, Lisa T., Eng, Christine M., Esteves, Cecilia, Estwick, Tyra, Fernandez, Liliana, Ferreira, Carlos, Fieg, Elizabeth L., Fisher, Paul G., Fogel, Brent L., Forghani, Irman, Fresard, Laure, Gahl, William A., Godfrey, Rena A., Goldman, Alica M., Goldstein, David B., Gourdine, Jean-Philippe F., Grajewski, Alana, Groden, Catherine A., Gropman, Andrea L., Haendel, Melissa, Hamid, Rizwan, Hanchard, Neil A., Hayes, Nichole, High, Frances, Holm, Ingrid A., Hom, Jason, Huang, Alden, Huang, Yong, Isasi, Rosario, Jamal, Fariha, Jiang, Yong-hui, Johnston, Jean M., Jones, Angela L., Karaviti, Lefkothea, Kelley, Emily G., Kiley, Dana, Koeller, David M., Kohane, Isaac S., Kohler, Jennefer N., Krakow, Deborah, Korrick, Susan, Koziura, Mary, Krier, Joel B., Kyle, Jennifer E., Lalani, Seema R., Lam, Byron, Lanpher, Brendan C., Lanza, Ian R., Lau, C. Christopher, Lazar, Jozef, LeBlanc, Kimberly, Lee, Brendan H., Lee, Hane, Levitt, Roy, Levy, Shawn E., Lewis, Richard A., Lincoln, Sharyn A., Liu, Pengfei, Liu, Xue Zhong, Loo, Sandra K., Maas, Richard L., Macnamara, Ellen F., MacRae, Calum A., Maduro, Valerie V., Majcherska, Marta M., Malicdan, May Christine V., Mamounas, Laura A., Manolio, Teri A., Mao, Rong, Markello, Thomas C., Marom, Ronit, Marth, Gabor, Martin, Beth A., Martin, Martin G., Martínez-Agosto, Julian A., Marwaha, Shruti, May, Thomas, McCauley, Jacob, McConkie-Rosell, Allyn, McCormack, Colleen E., McCray, Alexa T., Metz, Thomas O., Might, Matthew, Morava-Kozicz, Eva, Moretti, Paolo M., Morimoto, Marie, Mulvihill, John J., Murdock, David R., Nath, Avi, Nelson, Stan F., Newberry, J. Scott, Newman, John H., Nicholas, Sarah K., Novacic, Donna, Oglesbee, Devin, Orengo, James P., Pace, Laura, Pallais, J. Carl, Palmer, Christina G.S., Papp, Jeanette C., Parker, Neil H., Phillips, John A., III, Posey, Jennifer E., Postlethwait, John H., Potocki, Lorraine, Pusey, Barbara N., Quinlan, Aaron, Raja, Archana N., Renteria, Genecee, Reuter, Chloe M., Rives, Lynette, Robertson, Amy K., Rodan, Lance H., Rosenfeld, Jill A., Rowley, Robb K., Ruzhnikov, Maura, Sacco, Ralph, Sampson, Jacinda B., Samson, Susan L., Saporta, Mario, Schaechter, Judy, Schedl, Timothy, Schoch, Kelly, Scott, Daryl A., Shakachite, Lisa, Sharma, Prashant, Shashi, Vandana, Shields, Kathleen, Shin, Jimann, Signer, Rebecca, Sillari, Catherine H., Silverman, Edwin K., Sinsheimer, Janet S., Sisco, Kathy, Smith, Kevin S., Solnica-Krezel, Lilianna, Spillmann, Rebecca C., Stoler, Joan M., Stong, Nicholas, Sullivan, Jennifer A., Sutton, Shirley, Sweetser, David A., Tabor, Holly K., Tamburro, Cecelia P., Tan, Queenie K.-G., Tekin, Mustafa, Telischi, Fred, Thorson, Willa, Tifft, Cynthia J., Toro, Camilo, Tran, Alyssa A., Urv, Tiina K., Velinder, Matt, Viskochil, Dave, Vogel, Tiphanie P., Wahl, Colleen E., Walley, Nicole M., Walsh, Chris A., Walker, Melissa, Wambach, Jennifer, Wan, Jijun, Wang, Lee-kai, Wangler, Michael F., Ward, Patricia A., Waters, Katrina M., Webb-Robertson, Bobbie-Jo M., Wegner, Daniel, Westerfield, Monte, Wheeler, Matthew T., Wise, Anastasia L., Wolfe, Lynne A., Woods, Jeremy D., Worthey, Elizabeth A., Yamamoto, Shinya, Yang, John, Yoon, Amanda J., Yu, Guoyun, Zastrow, Diane B., Zhao, Chunli, Zuchner, Stephan, Bhatia, Aashim, Mobley, Bret C., Cogan, Joy, Koziura, Mary E., Phillips, John, Newman, John, and Moore, Steven A.

Published
2019
Full Text
View/download PDF

42. Léopold Sédar Senghor’s Translations and the Trajectory of a World Writer

Author

Shields, Kathleen

Subjects
French
Abstract

Senghor’s seldom-studied translations (grouped with his juvenilia and published in OEuvre poétique in 1964) illustrate a process of identity formation whereby he can variously represent France, Senegal, Africa, and poetry, ultimately coming to occupy a position as world writer. My analysis of these texts takes into account recent articles on translation and African writers: what did Senghor translate, how did he translate, why did he translate, and why did he stop translating? Strategies of universalizing, flattening, and mystification reveal a process of acculturation rather than cultural crossfertilization. Central to my argument is the contrast between Senghor’s concept of métissage and the more current use of the term as Antoine Berman applies it to translation. One optimistic narrative is that as decolonization takes place, more and more translation, increased mutual cultural understanding, and hybridization also occur. Senghor’s translations are part of another process, where translation from African languages is abandoned in favour of a world language and its tributaries. They also illustrate features common to other world writers. Once completed, there is no further need for translation to take place.

Published
2010

43. Why Bother with Languages?

Author

Shields, Kathleen and Kelly, T.A.F.

Abstract

Globalization has been described as the expansion of Western values to a world scale. The planet is now seen in its totality, whether through political and military surveillance, through observation of geological and climatic change, or through massive increase in human trade, communication and travel. One o f the features of globalization is the enlargement of economic zones without this necessarily bringing about the disappearance of borderlands and grey areas. Another feature is the uneven linking of different parts of the world through new forms of communication. The interconnectedness of peoples is often imposed by outside events over which they have little control. Finally, globalization has involved a reappraisal of old antagonisms (religious, ideological or nationalistic).

Published
2006

44. One Sentence: Illyes and Eluard

Author

Shields, Kathleen and Kabdebo, Thomas

Abstract

In the autumn 1995 number of The Hungarian Quarterly Matyas Domokos maintained- with circumstantial evidenc-that Gyula Illyes's most famous poem, "A sentence on Tyranny" was written in the early 1950s and not (as the Kadar regime claimed) during, or immediately before, the 1956 Revolution.' Domokos enlists the poet's utterances regarding the matter, and refers to the life of the poem extra Hungariam between its first publication in the heady days of that red autumn, and the demise of censorship in Hungary in the late eighties.

Published
1996

45. Three Irish translations of Rimbaud's 'Bateau Ivre'

Author

Shields, Kathleen

Subjects
French
Abstract

Three twentieth-century Irish writers have chosen to translate Rimbaud’s “Bateau ivre”: Samuel Beckett in the 1930s, Derek Mahon in the 1980s and Ciaran Carson in the 1990s. The first question that springs to mind is, why should these three choose a text that has as its subject exile and the weary longing to return?

Published
1995

46. "Challenges and Possibilities for World Literature, Global Literature, and Translation".

Author

SHIELDS, Kathleen

Subjects
LITERATURE, HUMANITIES, CENSORSHIP, CULTURE, AUTHORSHIP
Abstract

In her article "Challenges and Poßibilities for World Literature, Global Literature, and Translation" Kathleen Shields argues that Goethe's concept of Weltliteratur was grounded in translation practice: in creating a canon representing the best of each nation, translation occupied centre stage. Nation-building in Europe in the nineteenth century was combined with the idea of transnational literature where translation was an important tool of transmißion and exchange, as well as a way of decentering from a strong monolingual base. There are four challenges for comparative literature now. Firstly, the nation state is weakening. Secondly, despite the growing interest in world literature since the 1990s, Goethe's idea of Weltliteratur is in decline, replaced by the rise of English as a lingua franca and pivot language for translations. Thirdly, asymmetries in relations between languages, and the very small number of translation languages, are becoming more marked. Finally, digital reading means that literary products are simply "there," fluidly absorbed into ever-changing canons with no visible means of mediation. What then are the opportunities offered by the European tradition of translation studies? Could polysystem theory help to re-examine the filters and asymmetric relations that exist between producers and consumers of literary texts? Can translation studies map relations of prestige to arrive at not only an ethics but also a geopolitics of literature? [ABSTRACT FROM AUTHOR]

Published
2013
Full Text
View/download PDF

47. Diversity of proteobacterial endosymbionts in hemlock woolly adelgid ( Adelges tsugae) ( Hemiptera: Adelgidae) from its native and introduced range.

Author

Dohlen, Carol D., Spaulding, Usha, Shields, Kathleen, Havill, Nathan P., Rosa, Cristina, and Hoover, Kelli

Subjects
HEMLOCK woolly adelgid, PROTEOBACTERIA, ENDOSYMBIOSIS, INSECT microbiology, MICROBIAL variation, BACTERIAL genetics, INSECT populations, BACTERIAL typing
Abstract

Knowledge of intraspecific variation in symbioses may aid in understanding the ecology of widespread insects in different parts of their range. We investigated bacterial symbionts of Adelges tsugae, a pest of hemlocks in eastern North America introduced from Asia. Amplification, cloning, and sequencing of bacterial 16 S rDNA, in situ hybridizations, and electron microscopy revealed that A. tsugae harbours up to five bacterial phylotypes, according to population. Three Gammaproteobacteria species are maternally transmitted. The first, designated ' Ca. Pseudomonas adelgestsugas' resides in the haemocoel, and was detected in all populations except Taiwan. The second phylotype, ' Ca. Serratia symbiotica', resides in bacteriocytes of populations on Tsuga sieboldii in Japan and in E. North America. The third phylotype, designated ' Ca. Annandia adelgestsuga', clustered within a lineage of several insect endosymbionts that included Buchnera aphidicola. It was detected in bacteriocytes in all populations, and in salivary glands of first instars. Two Betaproteobacteria phylotypes were detected in some Japanese T. sieboldii and eastern North America populations, and were observed only in salivary glands with no evidence of maternal transmission. Our results support the ideas that symbiont gain and loss has been volatile in adelgids, and that symbionts may help to trace the source of invasive species. [ABSTRACT FROM AUTHOR]

Published
2013
Full Text
View/download PDF

48. A zymogen-like factor Xa variant corrects the coagulation defect in hemophilia.

Author

Ivanciu, Lacramioara, Toso, Raffaella, Margaritis, Paris, Pavani, Giulia, Kim, Haein, Schlachterman, Alexander, Liu, Jian-Hua, Clerin, Valerie, Pittman, Debra D, Rose-Miranda, Rosalind, Shields, Kathleen M, Erbe, David V, Tobin, James F, Arruda, Valder R, and Camire, Rodney M

Subjects
BLOOD coagulation, HEMOPHILIA, HEMOSTASIS, LABORATORY mice, HEMORRHAGE treatment
Abstract

Effective therapies are needed to control excessive bleeding in a range of clinical conditions. We improve hemostasis in vivo using a conformationally pliant variant of coagulation factor Xa (FXaI16L) rendered partially inactive by a defect in the transition from zymogen to active protease. Using mouse models of hemophilia, we show that FXaI16L has a longer half-life than wild-type FXa and does not cause excessive activation of coagulation. Once clotting mechanisms are activated to produce its cofactor FVa, FXaI16L is driven to the protease state and restores hemostasis in hemophilic animals upon vascular injury. Moreover, using human or murine analogs, we show that FXaI16L is more efficacious than FVIIa, which is used to treat bleeding in hemophilia inhibitor patients. FXaI16L may provide an effective strategy to enhance blood clot formation and act as a rapid pan-hemostatic agent for the treatment of bleeding conditions. [ABSTRACT FROM AUTHOR]

Published
2011
Full Text
View/download PDF

49. A Monoclonal Antibody Against RAGE Alters Gene Expression and is Protective in Experimental Models of Sepsis and Pneumococcal Pneumonia.

Author

Christaki, Eirini, Opal, Steven M., Keith Jr, James C., Kessimian, Nubar, Palardy, John E., Parejo, Nicolas A., Tan, Xiang Yang, Piche-Nicholas, Nicole, Tchistiakova, Lioudmila, Vlasuk, George P., Shields, Kathleen M., Feldman, Jeffrey L., Lavallie, Edward R., Arai, Maya, Mounts, William, and Pittman, Debra D.

Published
2011
Full Text
View/download PDF

50. Variation in Winter Survival of the Invasive Hemlock Woolly Adelgid (Hemiptera: Adelgidae) Across the Eastern United States.

Author

Trotter III, R. Talbot and Shields, Kathleen S.

Subjects
ENTOMOLOGY research, SURVIVAL behavior (Animals), HEMLOCK woolly adelgid, INSECT behavior, INTRODUCED species, TEMPERATURE & the environment, LOW temperatures, MORTALITY
Abstract

The hemlock woolly adelgid (Adelges tsugae Annand) is a small, aphid-like insect native to East Asia and western North America. First documented in the eastern United States in Richmond, VA, in 1951, it has spread to at least 17 states, where it causes increased mortality among both eastern and Carolina hemlocks (Tsuga canadensis Carrière and T. caroliniana Engelmann., respectively). Previous work has suggested low temperatures may limit northward spread of the adelgid. Using recent surveys of A. tsugae mortality across the infested latitudinal gradient of the eastern United States, we show there is a significant positive relationship between minimum winter temperatures and winter survival at the landscape scale. The strength and nature of this relationship, however, varies through time, with absolute minimum winter temperatures explaining almost one half of the tree-level variance in survival in the spring of 2004 but only 9% in 2003. Post hoc analyses of the data suggest the explanatory power of temperature can be improved in ongoing studies by examining seasonal temperature profiles. Previous studies have also suggested adelgid survival may be density dependent, and although these data support this observation, contemporary density is a poor predictor of adelgid survival at the landscape scale. Using landscape estimates of minimum winter temperature, we show two simple methods of estimating landscape-scale adelgid survival rates. Both methods suggest much of the range of T. canadensis in the eastern United States, and the entire range of T. caroliniana falls in areas where winter temperatures will not impose critical limits on A. tsvgae populations. [ABSTRACT FROM AUTHOR]

Published
2009
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results