109 results on '"Qingyong Meng"'
Search Results
2. Lactoferrin deficiency during lactation increases the risk of depressive-like behavior in adult mice
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Wenli Wang, Zhimei Cheng, Xiong Wang, Qin An, Kunlun Huang, Yunping Dai, Qingyong Meng, and Yali Zhang
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Lactation ,Lactoferrin ,Depression ,Microbial–intestinal–brain ,Innate immunity ,Inflammation ,Biology (General) ,QH301-705.5 - Abstract
Abstract Background Lactoferrin is an active protein in breast milk that plays an important role in the growth and development of infants and is implicated as a neuroprotective agent. The incidence of depression is currently increasing, and it is unclear whether the lack of lactoferrin during lactation affects the incidence of depressive-like behavior in adulthood. Results Lack of lactoferrin feeding during lactation affected the barrier and innate immune functions of the intestine, disrupted the intestinal microflora, and led to neuroimmune dysfunction and neurodevelopmental delay in the hippocampus. When exposed to external stimulation, adult lactoferrin feeding-deficient mice presented with worse depression-like symptoms; the mechanisms involved were activation of the LPS–TLR4 signalling pathway in the intestine and hippocampus, reduced BDNF-CREB signaling pathway in hippocampus, increased abundance of depression-related bacteria, and decreased abundance of beneficial bacteria. Conclusions Overall, our findings reveal that lactoferrin feeding deficient during lactation can increase the risk of depressive-like behavior in adults. The mechanism is related to the regulatory effect of lactoferrin on the development of the "microbial–intestinal–brain" axis.
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- 2023
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3. Immunoglobulin Superfamily Containing Leucine-Rich Repeat (ISLR) Serves as a Redox Sensor That Modulates Antioxidant Capacity by Suppressing Pyruvate Kinase Isozyme M2 Activity
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Tongtong Wang, Meijing Chen, Yang Su, Yuying Zhang, Chang Liu, Miaomiao Lan, Lei Li, Fan Liu, Na Li, Yingying Yu, Lei Xiong, Kun Wang, Jin Liu, Qing Xu, Yue Hu, Yuxin Jia, Yuxin Cao, Jingwen Pan, and Qingyong Meng
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oxidative stress ,ISLR ,redox balance ,pyruvate kinase ,Cytology ,QH573-671 - Abstract
Cells defend against oxidative stress by enhancing antioxidant capacity, including stress-activated metabolic alterations, but the underlying intracellular signaling mechanisms remain unclear. This paper reports that immunoglobulin superfamily containing leucine-rich repeat (ISLR) functions as a redox sensor that responds to reactive oxygen species (ROS) stimulation and modulates the antioxidant capacity by suppressing pyruvate kinase isozyme M2 (PKM2) activity. Following oxidative stress, ISLR perceives ROS stimulation through its cysteine residue 19, and rapidly degrades in the autophagy–lysosome pathway. The downregulated ISLR enhances the antioxidant capacity by promoting the tetramerization of PKM2, and then enhancing the pyruvate kinase activity, PKM2-mediated glycolysis is crucial to the ISLR-mediated antioxidant capacity. In addition, our results demonstrated that, in triple-negative breast cancer, cisplatin treatment reduced the level of ISLR, and PKM2 inhibition sensitizes tumors to cisplatin by enhancing ROS production; and argued that PKM2 inhibition can synergize with cisplatin to limit tumor growth. Our results demonstrate a molecular mechanism by which cells respond to oxidative stress and modulate the redox balance.
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- 2024
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4. Anti-Aging Effect of Hemerocallis citrina Baroni Polysaccharide-Rich Extract on Caenorhabditis elegans
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Yunxia Zou, Xiyue Qin, Wenli Wang, Qingyong Meng, and Yali Zhang
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Hemerocallis citrina Baroni ,polysaccharide ,C. elegans ,antioxidant ,longevity ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Plant polysaccharides are important for anti-aging research. Polysaccharides from Hemerocallis citrina Baroni (H. citrina) have been reported to have antioxidant activity; however, their anti-aging roles and mechanisms are not clear. In this study, we extracted polysaccharides from H. citrina by an ultrasonic-assisted water extraction–alcohol precipitation method and chemically determined the physicochemical properties such as extraction yield, content, and in vitro antioxidant properties of H. citrina polysaccharide-rich extract (HCPRE). Using Caenorhabditis elegans (C. elegans) as a model animal, the anti-aging effect of HCPRE was investigated, and the mechanism of action of HCPRE was explored by the in vivo antioxidant level assay of C. elegans and the related gene expression assay. The extraction yield of HCPRE was 11.26%, the total polysaccharide content was 77.96%, and the main monosaccharide components were glucose and galactose. In addition, HCPRE exhibited good antioxidant activity both in vitro and in vivo. Under normal thermal stress and oxidative stress conditions, being fed 1200 µg/mL of HCPRE significantly prolonged the life span of C. elegans by 32.65%, 17.71%, and 32.59%, respectively. Our study showed that HCPRE exerted an anti-aging effect on C. elegans, and its mechanism involves increasing the activities of catalase (CAT) and superoxide dismutase (SOD), reducing the level of reactive oxygen species (ROS) and regulating the expression of related genes.
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- 2024
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5. The elimination of stressed induced light leakage for in-plane-switching LCD
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Feifei Wang, Hongming Zhan, Lintao Ji, Tao Yang, Qingyong Meng, Yajun Li, Bowen Li, Yuansheng Zang, Junsheng Chen, Yinhu Huang, Kaixuan Wang, Lifeng Lin, and Xibin Shao
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Medicine ,Science - Abstract
Abstract As the mainstream display mode of LCD, IPS is overwhelmingly used in many fields of flat displays. However, due to the stress sensitivity of glass, the stressed light leakage is a bottleneck for achieving perfect dark state performance. The conventional scheme of using a compensation polarizer outside the cell has no effect on this light leakage. Although many studies have been conducted to overcome this limitation, the proposed methods have limited effects. Our research team has proposed a novel light leakage compensation mechanism by introducing a positive A plate that is sandwiched between the glass and the LC layer, therefore the light leakage which is caused by the combined effect of the phase retardations from the stressed glasses and the LC layer can be eliminated. In addition to theoretically analyzing the compensation principles of the novel light leakage compensation mechanism, we also use the developed positive A material to prepare light leakage compensation demos. And then the electric-optical characteristics and light leakage compensation effects of the demos are evaluated. While maintaining excellent optical and electrical characteristics, this technology effectively solves the problem of stressed light leakage of glass-based IPS, improves the dark-state image quality, and breaks the application of IPS in products such as curve products.
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- 2022
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6. Melatonin improves the first cleavage of parthenogenetic embryos from vitrified–warmed mouse oocytes potentially by promoting cell cycle progression
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Bo Pan, Izhar Hyder Qazi, Shichao Guo, Jingyu Yang, Jianpeng Qin, Tianyi Lv, Shengqin Zang, Yan Zhang, Changjun Zeng, Qingyong Meng, Hongbing Han, and Guangbin Zhou
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Cell cycle ,Cleavage rate ,Melatonin ,Metaphase II oocyte ,Parthenogenetic activation ,Vitrification ,Animal culture ,SF1-1100 ,Veterinary medicine ,SF600-1100 - Abstract
Abstract Background This study investigated the effect of melatonin (MT) on cell cycle (G1/S/G2/M) of parthenogenetic zygotes developed from vitrified-warmed mouse metaphase II (MII) oocytes and elucidated the potential mechanism of MT action in the first cleavage of embryos. Results After vitrification and warming, oocytes were parthenogenetically activated (PA) and in vitro cultured (IVC). Then the spindle morphology and chromosome segregation in oocytes, the maternal mRNA levels of genes including Miss, Doc1r, Setd2 and Ythdf2 in activated oocytes, pronuclear formation, the S phase duration in zygotes, mitochondrial function at G1 phase, reactive oxygen species (ROS) level at S phase, DNA damage at G2 phase, early apoptosis in 2-cell embryos, cleavage and blastocyst formation rates were evaluated. The results indicated that the vitrification/warming procedures led to following perturbations 1) spindle abnormalities and chromosome misalignment, alteration of maternal mRNAs and delay in pronucleus formation, 2) decreased mitochondrial membrane potential (MMP) and lower adenosine triphosphate (ATP) levels, increased ROS production and DNA damage, G1/S and S/G2 phase transition delay, and delayed first cleavage, and 3) increased early apoptosis and lower levels of cleavage and blastocyst formation. Our results further revealed that such negative impacts of oocyte cryopreservation could be alleviated by supplementation of warming, recovery, PA and IVC media with 10− 9 mol/L MT before the embryos moved into the 2-cell stage of development. Conclusions MT might promote cell cycle progression via regulation of MMP, ATP, ROS and maternal mRNA levels, potentially increasing the first cleavage of parthenogenetic zygotes developed from vitrified–warmed mouse oocytes and their subsequent development.
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- 2021
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7. Selection and validation of suitable reference genes in adipose tissue of Jianzhou Da'er Goat (Capra hircus)
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Guangjie Xie, Lu Tang, Yaqiu Lin, Mingfeng Jiang, Qing Xu, Jiangjiang Zhu, Qingyong Meng, and Yong Wang
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goat ,adipose tissue ,reference genes ,gene expression stability ,Veterinary medicine ,SF600-1100 - Abstract
Reverse transcription quantitative real-time PCR (RT-qPCR) is a common and high-efficiency technique for detecting the mRNA levels of genes where suitable reference genes were introduced to normalize the data of RT-qPCR. However, little is known about the suitable reference genes in the adipose tissue of Jianzhou da’er goat. Here, six housekeeping genes, including the ACTB, ALAS1, EIF3K, HSP90, RPLP0, and UXT, were selected as candidate reference genes, while the FASN, HSL, LPL, and PID1 were used as the target genes for the validation of the suitability of identified reference genes. After detecting the expression levels of selected genes, the geNorm, NormFinder, and RefFinder softwares were applied to analyze the obtained data of candidate reference genes. The suitability analysis showed that the HSP90 was the most stable candidate reference gene, followed by the ALAS1 and RPLP0 gene. The validation experiment not only verified that the HSP90 was more stable than ACTB as the reference gene, but also confirmed that the HSP90 alone and a combination of HSP90, ALAS1, and RPLP0 could play the same roles in the normalization of target genes. Therefore, we strongly suggested that the HSP90 alone and the combination of three genes (HSP90, ALAS1, and RPLP0) could be used as the suitable reference for normalizing gene expression data in adipose tissue of Jianzhou da’er goat.
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- 2021
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8. Phenolic Compounds from Sonchus arvensis Linn. and Hemerocallis citrina Baroni. Inhibit Sucrose and Stearic Acid Induced Damage in Caenorhabditis elegans
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Qin An, Lei Zhang, Xiyue Qin, Xiong Wang, Wenli Wang, Qingyong Meng, and Yali Zhang
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Sonchus arvensis Linn. ,Hemerocallis citrina Baroni. ,phenolic compounds ,Caenorhabditis elegans ,antioxidant ,Organic chemistry ,QD241-441 - Abstract
Sonchus arvensis Linn. and Hemerocallis citrina Baroni. have been reported to improve body resistance. However, the underlying mechanism is not clear. In this study, Sonchus arvensis Linn. phenolic compounds (SAP) and Hemerocallis citrina Baroni. phenolic compounds (HCP) were extracted and their protective effects in Caenorhabditis elegans evaluated. SAP and HCP showed considerably different phenolic compositions. In the normal C. elegans model, HCP exhibited better effects in promoting growth than SAP. In the sucrose-incubated C. elegans model, both SAP and HCP showed positive effects against the high-sucrose-induced damage. In the stearic acid-incubated C. elegans model, both SAP and HCP improved lifespan, reproductive ability and growth, while HCP had a more evident effect than SAP on reproductive ability. The TGF-β signaling pathway was confirmed to be involved in the protective effects of SAP and HCP. The antioxidant ability of SAP was also found to be related to skn-1. Our study shows that both SAP and HCP have protective effects against high sucrose- or high stearic acid-induced damage.
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- 2023
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9. Author Correction: MiR-31 promotes mammary stem cell expansion and breast tumorigenesis by suppressing Wnt signaling antagonists
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Cong Lv, Fengyin Li, Xiang Li, Yuhua Tian, Yue Zhang, Xiaole Sheng, Yongli Song, Qingyong Meng, Shukai Yuan, Liming Luan, Thomas Andl, Xu Feng, Baowei Jiao, Mingang Xu, Maksim V. Plikus, Xing Dai, Christopher Lengner, Wei Cui, Fazheng Ren, Jianwei Shuai, Sarah E. Millar, and Zhengquan Yu
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Science - Abstract
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
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- 2020
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10. Anti‐TSNARE1 IgG plasma levels differ by sex in patients with schizophrenia in a Chinese population
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Chan Li, Ruth Whelan, Hua Yang, Yaling Jiang, Chaosen Qiu, Qingyong Meng, and Jun Wei
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autoantibodies ,B lymphocytes ,ELISA ,gender difference ,schizophrenia ,TSNARE1 ,Biology (General) ,QH301-705.5 - Abstract
It was recently reported that levels of plasma IgG antibodies against peptide antigens derived from proteins encoded by schizophrenia‐associated genes are altered in individuals with schizophrenia treated with antipsychotics. This study aimed to replicate the initial finding in antipsychotic‐naïve patients with first‐episode schizophrenia and to explore the possible mechanism by which immune tolerance of B cells may be altered in this disease. A total of 408 case–control plasma samples were collected for analysis of circulating IgG antibodies against fragments derived from TCF4, TSNARE1, ZNF804A, TRANK1, ERCC4, DPYD and CD25 using an in‐house ELISA. The Mann–Whitney U‐test revealed that patients with schizophrenia had a significant change in plasma anti‐TSNARE1 and anti‐CD25 IgG levels; male patients mainly contributed to the increased levels of anti‐TSNARE1 IgG and anti‐CD25 IgG. Receiver operating characteristic (ROC) curve analysis revealed that the anti‐TSNARE1 IgG assay had an area under the ROC curve of 0.625 with a sensitivity of 15.7% and a specificity of 95.2%. Work on a B‐cell model revealed that TRANK1‐derived antigen treatments could enhance the proportions of CD83+ cells and apoptotic B cells when compared with TSNARE1‐derived antigen and vehicle treatment. We conclude that there is a gender difference in autoimmune responses in schizophrenia and suggest that anti‐TSNARE1 IgG may be indicative of schizophrenia in a subgroup of male patients.
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- 2019
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11. Circulating Natural Autoantibodies to HER2-Derived Peptides Performed Antitumor Effects on Oral Squamous Cell Carcinoma
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Xiu Liu, Ziyi He, Yi Qu, Qingyong Meng, Lizheng Qin, and Ying Hu
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circulating natural autoantibodies ,HER2 ,oral squamous cell carcinoma ,trastuzumab ,intravenous immunoglobulin ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Natural autoantibodies play a crucial role in destruction of malignant tumors due to immune surveillance function. Epidermal growth factor receptor 2 (HER2) has been found to be highly expressed in a variety of epithelial tumors including oral squamous cell carcinoma (OSCC). The present study was thus undertaken to investigate the effect of anti-HER2 natural autoantibodies on OSCC. Compared with cancer-adjacent tissues, cancer tissues from OSCC patients exhibited higher HER2 expression especially in those with middle & advanced stage OSCC. Plasma anti-HER2 IgG levels examined with an enzyme-linked immunosorbent assay (ELISA) developed in-house showed differences between control subjects, individuals with oral benign tumor and patients with OSCC. In addition, anti-HER2 IgG-abundant plasma was screened from healthy donors to treat OSCC cells and to prepare for anti-HER2 intravenous immunoglobulin (IVIg). Both anti-HER2 IgG-abundant plasma and anti-HER2 IVIg could significantly inhibit proliferation and invasion of OSCC cells by inducing the apoptosis, and also regulate apoptosis-associated factors and epithelial-mesenchymal transition (EMT), respectively. Besides, the complement-dependent cytotoxicity (CDC) pathway was likely to contribute to the anti-HER2 IgG mediated inhibition of OSCC cells. After the HER2 gene was knocked down with HER2-specific siRNAs, the inhibitory effects on OSCC cell proliferation and apoptotic induction faded away. In conclusion, human plasma IgG, or IVIg against HER2 may be a promising agent for anti-OSCC therapy.
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- 2021
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12. Ectopic Expression of VvSUC27 Induces Stenospermocarpy and Sugar Accumulation in Tomato Fruits
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Yumeng Cai, Ling Yin, Wenrui Tu, Zhefang Deng, Jing Yan, Wenjie Dong, Han Gao, Jinxu Xu, Nan Zhang, Jie Wang, Lei Zhu, Qingyong Meng, and Yali Zhang
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Vitis vinifera ,VvSUC ,overexpression ,tomato ,stenospermocarpy ,transcriptomic analysis ,Plant culture ,SB1-1110 - Abstract
Seedless fruits are favorable in the market because of their ease of manipulation. Sucrose transporters (SUTs or SUCs) are essential for carbohydrate metabolism in plants. Whether SUTs participate directly in causing stenospermocarpy, thereby increasing fruit quality, remains unclear. Three SUTs, namely, VvSUC11, VvSUC12, and VvSUC27 from Vitis vinifera, were characterized and ectopic expression in tomatoes. VvSUC11- and VvSUC12-overexpressing lines had similar flower and fruit phenotypes compared with those of the wild type. VvSUC27-overexpressing lines produced longer petals and pistils, an abnormal stigma, much less and shrunken pollen, and firmer seedless fruits. Moreover, produced fruits from all VvSUC-overexpressing lines had a higher soluble solid content and sugar concentration. Transcriptomic analysis revealed more genes associated with carbohydrate metabolism and sugar transport and showed downregulation of auxin- and ethylene-related signaling pathways during early fruit development in VvSUC27-overexpressing lines relative to that of the wild type. Our findings demonstrated that stenospermocarpy can be induced by overexpression of VvSUC27 through a consequential reduction in nutrient delivery to pollen at anthesis, with a subsequent downregulation of the genes involved in carbohydrate metabolism and hormone signaling. These commercially desirable results provide a new strategy for bioengineering stenospermocarpy in tomatoes and in other fruit plants.
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- 2021
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13. Immunoglobulin Superfamily Containing Leucine-Rich Repeat (Islr) Participates in IL-6-Mediated Crosstalk between Muscle and Brown Adipose Tissue to Regulate Energy Homeostasis
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Chang Liu, Jin Liu, Tongtong Wang, Yang Su, Lei Li, Miaomiao Lan, Yingying Yu, Fan Liu, Lei Xiong, Kun Wang, Meijing Chen, Na Li, Qing Xu, Yue Hu, Yuxin Jia, and Qingyong Meng
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BAT-muscle cross talk ,energy expenditure ,Islr ,muscle-derived IL-6 ,mitochondrial function ,Ndufs2 ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Brown adipose tissue (BAT) is functionally linked to skeletal muscle because both tissues originate from a common progenitor cell, but the precise mechanism controlling muscle-to-brown-fat communication is insufficiently understood. This report demonstrates that the immunoglobulin superfamily containing leucine-rich repeat (Islr), a marker of mesenchymal stromal/stem cells, is critical for the control of BAT mitochondrial function and whole-body energy homeostasis. The mice loss of Islr in BAT after cardiotoxin injury resulted in improved mitochondrial function, increased energy expenditure, and enhanced thermogenesis. Importantly, it was found that interleukin-6 (IL-6), as a myokine, participates in this process. Mechanistically, Islr interacts with NADH: Ubiquinone Oxidoreductase Core Subunit S2 (Ndufs2) to regulate IL-6 signaling; consequently, Islr functions as a brake that prevents IL-6 from promoting BAT activity. Together, these findings reveal a previously unrecognized mechanism for muscle-BAT cross talk driven by Islr, Ndufs2, and IL-6 to regulate energy homeostasis, which may be used as a potential therapeutic target in obesity.
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- 2022
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14. Lactoferrin Deficiency Impairs Proliferation of Satellite Cells via Downregulating the ERK1/2 Signaling Pathway
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Xiong Wang, Fan Liu, Qin An, Wenli Wang, Zhimei Cheng, Yunping Dai, Qingyong Meng, and Yali Zhang
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lactoferrin ,skeletal muscle ,satellite cells ,proliferation ,ERK1/2 ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Lactoferrin (Ltf), a naturally active glycoprotein, possesses anti-inflammatory, anti-microbial, anti-tumor, and immunomodulatory activities. Many published studies have indicated that Ltf modulates the proliferation of stem cells. However, the role of Ltf in the proliferation of satellite cells, an important cell type in muscle regeneration, has not yet been reported. Here, by using Ltf systemic knockout mice, we illustrate the role of Ltf in skeletal muscle. Results shows that Ltf deficiency impaired proliferation of satellite cells (SCs) and the regenerative capability of skeletal muscle. Mechanistic studies showed that ERK1/2 phosphorylation was significantly downregulated after Ltf deletion in SCs. Simultaneously, the cell cycle-related proteins cyclin D and CDK4 were significantly downregulated. Intervention with exogenous recombinant lactoferrin (R-Ltf) at a concentration of 1000 μg/mL promoted proliferation of SCs. In addition, intraperitoneal injection of Ltf effectively ameliorated the skeletal muscle of mice injured by 1.2% BaCl2 solution. Our results suggest a protective effect of Ltf in the repair of skeletal muscle damage. Ltf holds promise as a novel therapeutic agent for skeletal muscle injuries.
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- 2022
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15. A Hardware and Software Task-Scheduling Framework Based on CPU+FPGA Heterogeneous Architecture in Edge Computing
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Zongwei Zhu, Junneng Zhang, Jinjin Zhao, Jing Cao, Duan Zhao, Gangyong Jia, and Qingyong Meng
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FPGA ,edge computing ,task scheduling ,heterogeneous system ,dynamic partial reconfigurable system ,Electrical engineering. Electronics. Nuclear engineering ,TK1-9971 - Abstract
Real-time performance is the primary requirement for edge computing systems. However, with the surge in data volume and the growing demand for computing power, a computing framework consisting solely of CPUs is no longer competent. As a result, CPU+ heterogeneous architecture using accelerators to improve edge computing systems' computing capacity has received great attention. The type of accelerators determines the performance of the edge computing system largely. The accelerators include Graphics Processing Unit (GPU), Application Specific Integrated Circuit (ASIC) and Field Programmable Gate Array (FPGA). FPGAs with its reconfigurability and high energy efficiency are widely used in many edge computing scenarios. Nontheless, the performance depends also on the scheduling efficiency between software tasks on CPUs and hardware tasks on FPGAs. Unfortunately, the existing strategies have not fully exploited the differences between hardware and software tasks, thus resulting in low scheduling efficiency. This paper proposes a task scheduling framework on the Dynamic Partial Reconfiguration (DPR) platform. We take full account of the characteristics of task switching overhead and predictable execution time of hardware tasks in DPR, and reduce the number of task-switching times and active tasks in the system, thus improving the scheduling efficiency. We conduct a set of experiments on the Zynq platform to verify the proposed framework. Experimental results demonstrate that when the execution time of the accelerator exceeds the reconfiguration cost by an order of magnitude, the efficiencies of all the cases are more than 98%, and the efficiencies can reach 90%-98% in the same order of magnitude.
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- 2019
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16. Islr regulates canonical Wnt signaling-mediated skeletal muscle regeneration by stabilizing Dishevelled-2 and preventing autophagy
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Kuo Zhang, Yuying Zhang, Lijie Gu, Miaomiao Lan, Chuncheng Liu, Meng Wang, Yang Su, Mengxu Ge, Tongtong Wang, Yingying Yu, Chang Liu, Lei Li, Qiuyan Li, Yaofeng Zhao, Zhengquan Yu, Fudi Wang, Ning Li, and Qingyong Meng
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Science - Abstract
“Satellite cells are crucial for skeletal muscle regeneration. Here the authors show that immunoglobulin superfamily containing leucine-rich repeat (Islr) promotes skeletal muscle regeneration via a mechanism involving Dishevelled-2 stabilization in satellite cells and protection from autophagy.
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- 2018
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17. miR-29a/b1 Inhibits Hair Follicle Stem Cell Lineage Progression by Spatiotemporally Suppressing WNT and BMP Signaling
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Mengxu Ge, Chuncheng Liu, Lei Li, Miaomiao Lan, Yingying Yu, Lijie Gu, Yang Su, Kuo Zhang, Yuying Zhang, Tongtong Wang, Chang Liu, Fan Liu, Min Li, Lei Xiong, Kun Wang, Ting He, Yunping Dai, Yaofeng Zhao, Ning Li, Zhengquan Yu, and Qingyong Meng
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Biology (General) ,QH301-705.5 - Abstract
Summary: Hair follicle stem cells (HFSCs) and subsequent generations of matrix progeny make lineage choices by responding to spatiotemporal signals; however, the cues driving that specification are not well understood. Here, we demonstrate that the dynamics of microRNA (miR)-29 expression are inversely proportional to HFSC lineage progression. Furthermore, we show that sustained miR-29a/b1 overexpression in anagen or telogen in mice causes a short-hair phenotype and eventual hair loss by inhibiting the proliferation of HFSCs and matrix cells and likely preventing their differentiation. Conversely, in a loss-of-function in vivo model, miR-29a/b1 deficiency accelerates HFSC lineage progression in telogen. Mechanistically, miR-29a/b1 blocks HFSC lineage specification by spatiotemporally targeting Ctnnb1, Lrp6, Bmpr1a, and Ccna2. We further show that skin-specific Lrp6 or Bmpr1a ablation partially accounts for the short-hair phenotype. Overall, these synergistic targets reveal miR-29a/b1 as a high-fidelity antagonist of HFSC lineage progression and a potential therapeutic target for hair loss. : By using miR-29a/b1 gain- and loss-of-function mouse models, Ge et al. reveal that miR-29a/b1 suppresses lineage progression of hair follicle stem cells and matrix cells by spatiotemporally synergistic targets, Lrp6, Ctnnb1, and Bmpr1a. Keywords: miR-29a/b1, HFSC lineage progression, matrix, proliferation and differentiation, short hair, spatiotemporal targets, Lrp6, Bmpr1a, WNT pathway, BMP pathway
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- 2019
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18. Incorporation of a skeletal muscle-specific enhancer in the regulatory region of Igf1 upregulates IGF1 expression and induces skeletal muscle hypertrophy
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Yunlong Zou, Yanjun Dong, Qingyong Meng, Yaofeng Zhao, and Ning Li
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Medicine ,Science - Abstract
Abstract In this study, we upregulated insulin-like growth factor-1 (IGF1) expression specifically in skeletal muscle by engineering an enhancer into its non-coding regions and verified the expected phenotype in a mouse model. To select an appropriate site for introducing a skeletal muscle-specific myosin light chain (MLC) enhancer, three candidate sites that exhibited the least evolutionary conservation were chosen and validated in C2C12 single-cell colonies harbouring the MLC enhancer at each site. IGF1 was dramatically upregulated in only the site 2 single-cell colony series, and it exhibited functional activity leading to the formation of extra myotubes. Therefore, we chose site 2 to generate a genetically modified (GM) mouse model with the MLC enhancer incorporated by CRISPR/Cas9 technology. The GM mice exhibited dramatically elevated IGF1 levels, which stimulated downstream pathways in skeletal muscle. Female GM mice exhibited more conspicuous muscle hypertrophy than male GM mice. The GM mice possessed similar circulating IGF1 levels and tibia length as their WT littermates; they also did not exhibit heart abnormalities. Our findings demonstrate that genetically modifying a non-coding region is a feasible method to upregulate gene expression and obtain animals with desirable traits.
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- 2018
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19. Hetero/Homo-Complexes of Sucrose Transporters May Be a Subtle Mode to Regulate Sucrose Transportation in Grape Berries
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Yumeng Cai, Ling Yin, Jie Wang, Wenjie Dong, Han Gao, Jinxu Xu, Zhefang Deng, Wenrui Tu, Jing Yan, Qingyong Meng, and Yali Zhang
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different varieties ,Vitis ,sucrose transporter ,functional variation ,oligomerization ,functional regulation ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
The sugar distribution mechanism in fruits has been the focus of research worldwide; however, it remains unclear. In order to elucidate the relevant mechanisms in grape berries, the expression, localization, function, and regulation of three sucrose transporters were studied in three representative Vitis varieties. Both SUC11 and SUC12 expression levels were positively correlated with sugar accumulation in grape berries, whereas SUC27 showed a negative relationship. The alignment analysis and sucrose transport ability of isolated SUCs were determined to reflect coding region variations among V. vinifera, V. amurensis Ruper, and V. riparia, indicating that functional variation existed in one SUT from different varieties. Furthermore, potentially oligomerized abilities of VvSUCs colocalized in the sieve elements of the phloem as plasma membrane proteins were verified. The effects of oligomerization on transport properties were characterized in yeast. VvSUC11 and VvSUC12 are high-affinity/low-capacity types of SUTs that stimulate each other by upregulating Vmax and Km, inhibiting sucrose transport, and downregulating the Km of VvSUC27. Thus, changes in the distribution of different SUTs in the same cell govern functional regulation. The activation and inhibition of sucrose transport could be achieved in different stages and tissues of grape development to achieve an effective distribution of sugar.
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- 2021
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20. Detection of circulating natural antibodies to inflammatory cytokines in type-2 diabetes and clinical significance
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Weiyi Cai, Cailing Qiu, Hongyu Zhang, Xiangyun Chen, Xuan Zhang, Qingyong Meng, and Jun Wei
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Natural antibodies ,IgG antibody ,Inflammatory cytokines ,Type-2 diabetes ,ELISA ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Abstract Background Inflammatory cytokines have been demonstrated to be involved in developing insulin resistance and type-2 diabetes (T2D). Natural antibodies in the circulation have protective effects on common diseases in humans. The present study was thus designed to test the hypothesis that natural antibodies against inflammatory cytokines could be associated with T2D. Methods An enzyme-linked immunosorbent assay (ELISA) was developed in-house to detect plasma IgG against peptide antigens derived from interleukin 1α (IL1α), IL1β, IL6, IL8 and tumor necrosis factor-α (TNF-α) in 200 patients with T2D and 220 control subjects. Results Binary regression showed that compared with control subjects, T2D patients had a decreased level of plasma anti-IL6 IgG (adjusted r 2=0.034, p=0.0001), anti-IL8 IgG (adjusted r 2=0.021, p=0.002) and anti-TNF-α IgG (adjusted r 2=0.017, p=0.003). Female patients mainly contributed to decreased levels of anti-IL6 IgG (adjusted r 2=0.065, p=0.0008) and anti-IL8 IgG (adjusted r 2=0.056, p=0.003), while male patients mainly contributed to decreased anti-TNF-α IgG levels (adjusted r 2=0.024, p=0.005). ROC curve analysis revealed a sensitivity of 16.5% against specificity of 95.5% for anti-IL6 IgG assay and a sensitivity of 19.5% against specificity of 95.9% for anti-IL8 IgG assay. Glycated hemoglobin levels measured after 6-month glucose-lowering treatment appeared to be inversely correlated with plasma anti-IL1α IgG (r=-0.477, df=17, p=0.039) and anti-IL6 IgG (r=-0.519, df=17, p=0.023) although such correlation failed to survive the Bonferroni correction. Conclusions Deficiency of natural IgG against inflammatory cytokines is likely to be a risk factor for T2D development and detection of such antibodies may be useful for personalized treatment of the disease.
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- 2017
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21. MiR-31 promotes mammary stem cell expansion and breast tumorigenesis by suppressing Wnt signaling antagonists
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Cong Lv, Fengyin Li, Xiang Li, Yuhua Tian, Yue Zhang, Xiaole Sheng, Yongli Song, Qingyong Meng, Shukai Yuan, Liming Luan, Thomas Andl, Xu Feng, Baowei Jiao, Mingang Xu, Maksim V. Plikus, Xing Dai, Christopher Lengner, Wei Cui, Fazheng Ren, Jianwei Shuai, Sarah E. Millar, and Zhengquan Yu
- Subjects
Science - Abstract
MicroRNAs play an important role in stem cell fate and tumorigenesis. In this work, the authors show that miR-31 controls mammary stem cell self-renewal and tumorigenesis by simultaneously activating Wnt/β-catenin and repressing TGFβ signaling pathways.
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- 2017
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22. Analysis of the Dynamic Modeling Method of Articulated Vehicles
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Qingyong Meng, Lulu Gao, Heping Xie, and Fengqian Dou
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Engineering (General). Civil engineering (General) ,TA1-2040 ,Technology (General) ,T1-995 - Published
- 2017
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23. Expression of Sucrose Transporters from Vitis vinifera Confer High Yield and Enhances Drought Resistance in Arabidopsis
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Yumeng Cai, Jing Yan, Wenrui Tu, Zhefang Deng, Wenjie Dong, Han Gao, Jinxu Xu, Nan Zhang, Ling Yin, Qingyong Meng, and Yali Zhang
- Subjects
Vitis vinifera ,VvSUC ,overexpression ,Arabidopsis ,srong phenotype ,high production ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Sucrose is the predominant form of sugar transported from photosynthetic (source) to non-photosynthetic (sink) organs in higher plants relying on the transporting function of sucrose transporters (SUTs or SUCs). Many SUTs have been identified and characterized in both monocots and dicots. However, the function of sucrose transporters (SUTs or SUCs) from Vitis is not clear. As the world’s most planted grape species, Vitis vinifera owns three sucrose transport activity verified SUTs. In this study, we constructed three kinds of VvSUC (Vitis vinifera SUC)-overexpressing transgenic Arabidopsis. VvSUC-overexpressing transgenic Arabidopsis was cultured on sucrose-supplemented medium. VvSUC11- and VvSUC12-overexpressing lines had similar thrived growth phenotypes, whereas the size and number of leaves and roots from VvSUC27-overexpressing lines were reduced compared with that of WT. When plants were cultured in soil, all SUT transgenic seedlings produced more number of leaves and siliques, resulting in higher yield (38.6% for VvSUC12-transformants) than that of WT. Besides, VvSUC27-transformants and VvSUC11-transformants enhanced drought resistance in Arabidopsis, providing a promising target for crop improvement
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- 2020
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24. MiR-18a regulates myoblasts proliferation by targeting Fgf1.
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Chuncheng Liu, Min Chen, Meng Wang, Wenhui Pi, Ning Li, and Qingyong Meng
- Subjects
Medicine ,Science - Abstract
MiRNAs play an important role in cell proliferation, apoptosis, and differentiation. MiR-18a is increasingly being recognized as a regulator of cancer pathogenesis. Here, we discovered that miR-18a participates in myoblasts proliferation. Expression of miR-18a was downregulated with the differentiation of C2C12 myoblasts. Overexpression of miR-18a affected the proliferation of C2C12 cells, primary myoblasts and RD cells. MiR-18a influenced the expression of cell cycle-related genes. Using TargetScan 6.2, we found that the 3' untranslated region (UTR) of the mouse Fgf1 gene contains complementary sequences to miR-18a. Using a siRNA, we confirmed that the reduction in the Fgf1 levels inhibited proliferation of C2C12 cells. Therefore, our results show that miR-18a participates in the regulation of proliferation by partly decreasing the expression of Fgf1.
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- 2018
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25. Study of circulating IgG antibodies to BIRC5 and MYC in non‐small cell lung cancer
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Leiguang Ye, Weili Wang, Cairen Chen, Qingyong Meng, and Yan Yu
- Subjects
Lung cancer ,BIRC5 ,MYC ,Autoantibodies ,ELISA ,Tumor immunity ,Biology (General) ,QH301-705.5 - Abstract
An in‐house enzyme‐linked immunosorbent assay (ELISA) was developed in this study to detect circulating IgG antibodies to peptide antigens derived from baculoviral IAP repeat‐containing protein 5 isoform 2 (BIRC5) and myc proto‐oncogene protein (MYC) in non‐small cell lung cancer (NSCLC). Student'st‐test revealed that circulating anti‐MYC IgG levels were significantly increased in patients with NSCLC compared with control subjects in the discovery sample (t = 3.96,P = 0.0001) but not in the validation sample (t = 1.24,P = 0.217), generating a combinedP‐value of 0.0003. Neither the discovery sample nor the validation sample showed a significant change in anti‐BIRC5 IgG levels in NSCLC. Further analysis was performed to investigate whether circulating IgG antibodies to these two tumor‐associated antigens (TAAs) significantly changed with early (stages I + II) and late (stages III + IV) NSCLC stages. The results showed that neither anti‐MYC IgG nor anti‐BIRC5 IgG levels significantly changed in patients with early stage NSCLC, while patients with late stage NSCLC had higher levels of circulating anti‐MYC IgG than control subjects in the discovery sample (t = 4.74,P < 0.0001) but not in the validation sample (t = 0.80,P = 0.423), generating a combinedP‐value of 0.00003 (X2= 26.13,df = 4). In conclusion, circulating IgG antibodies to MYC and BIRC5 do not appear to serve as biomarkers for early diagnosis of lung cancer but anti‐MYC IgG might have a prognostic value.
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- 2015
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26. Melatonin Improves In Vitro Development of Vitrified-Warmed Mouse Germinal Vesicle Oocytes Potentially via Modulation of Spindle Assembly Checkpoint-Related Genes
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Zhenzheng Wu, Bo Pan, Izhar Hyder Qazi, Haoxuan Yang, Shichao Guo, Jingyu Yang, Yan Zhang, Changjun Zeng, Ming Zhang, Hongbing Han, Qingyong Meng, and Guangbin Zhou
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melatonin ,oocyte vitrification ,redox homeostasis ,mitochondrial membrane potential ,ATP content ,spindle assembly checkpoint ,mouse ,Cytology ,QH573-671 - Abstract
The present study aimed to investigate the effect of melatonin (MT) supplementation on in vitro maturation of vitrified mouse germinal vesicle (GV) oocytes. The fresh oocytes were randomly divided into three groups: untreated (control), or vitrified by open-pulled straw method without (vitrification group) or with MT supplementation (vitrification + MT group). After warming, oocytes were cultured in vitro, then the reactive oxygen species (ROS) and glutathione (GSH) levels, mitochondrial membrane potential, ATP levels, spindle morphology, mRNA expression of spindle assembly checkpoint (SAC)-related genes (Mps1, BubR1, Mad1, Mad2), and their subsequent developmental potential in vitro were evaluated. The results showed that vitrification/warming procedures significantly decreased the percentage of GV oocytes developed to metaphase II (MII) stage, the mitochondrial membrane potential, ATP content, and GSH levels, remarkably increased the ROS levels, and significantly impaired the spindle morphology. The expressions of SAC-related genes were also altered in vitrified oocytes. However, when 10−7 mol/L MT was administered during the whole length of the experiment, the percentage of GV oocytes matured to MII stage was significantly increased, and the other indicators were also significantly improved and almost recovered to the normal levels relative to the control. Thus, we speculate that MT might regulate the mitochondrial membrane potential, ATP content, ROS, GSH, and expression of SAC-related genes, potentially increasing the in vitro maturation of vitrified-warmed mouse GV oocytes.
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- 2019
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27. Role of Selenium and Selenoproteins in Male Reproductive Function: A Review of Past and Present Evidences
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Izhar Hyder Qazi, Christiana Angel, Haoxuan Yang, Evangelos Zoidis, Bo Pan, Zhenzheng Wu, Zhang Ming, Chang-Jun Zeng, Qingyong Meng, Hongbing Han, and Guangbin Zhou
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male fertility ,mammalian reproduction ,selenium ,selenoproteins ,spermatogenesis ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Selenium (Se) is an important trace mineral having many essential roles at the cellular and organismal levels in animal and human health. The biological effects of Se are mainly carried out by selenoproteins (encoded by 25 genes in humans and 24 in mice). As an essential component of selenoproteins, Se performs structural and enzymic roles; in the latter context it is well known for its catalytic and antioxidative functions. Studies involving different animal models have added great value to our understanding regarding the potential implications of Se and selenoproteins in mammalian fertility and reproduction. In this review, we highlight the implications of selenoproteins in male fertility and reproduction followed by the characteristic biological functions of Se and selenoproteins associated with overall male reproductive function. It is evident from observations of past studies (both animal and human) that Se is essentially required for spermatogenesis and male fertility, presumably because of its vital role in modulation of antioxidant defense mechanisms and other essential biological pathways and redox sensitive transcription factors. However, bearing in mind the evidences from mainstream literature, it is also advisable to perform more studies focusing on the elucidation of additional roles played by the peculiar and canonical selenoproteins i.e., glutathione peroxidase 4 (GPX4) and selenoprotein P (SELENOP) in the male reproductive functions. Nevertheless, search for the elucidation of additional putative mechanisms potentially modulated by other biologically relevant selenoproteins should also be included in the scope of future studies. However, as for the implication of Se in fertility and reproduction in men, though a few clinical trials explore the effects of Se supplementation on male fertility, due to inconsistencies in the recruitment of subjects and heterogeneity of designs, the comparison of such studies is still complicated and less clear. Therefore, further research focused on the roles of Se and selenoproteins is awaited for validating the evidences at hand and outlining any therapeutic schemes intended for improving male fertility. As such, new dimensions could be added to the subject of male fertility and Se supplementation.
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- 2019
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28. Overexpression of miR-29 Leads to Myopathy that Resemble Pathology of Ullrich Congenital Muscular Dystrophy
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Chuncheng Liu, Lei Li, Mengxu Ge, Lijie Gu, Meng Wang, Kuo Zhang, Yang Su, Yuying Zhang, Chang Liu, Miaomiao Lan, Yingying Yu, Tongtong Wang, Qiuyan Li, Yaofeng Zhao, Zhengquan Yu, Ning Li, and Qingyong Meng
- Subjects
UCMD ,disease model ,miR-29 ,dysplasia ,collagen ,Cytology ,QH573-671 - Abstract
Ullrich congenital muscular dystrophy (UCMD) bring heavy burden to patients’ families and society. Because the incidence of this disease is very low, studies in patients are extremely limited. Animal models of this disease are indispensable. UCMD belongs to extracellular matrix-related diseases. However, the disease models constructed by knocking out some pathogenic genes of human, such as the Col6a1, Col6a2, or Col6a3 gene, of mice could not mimic UCMD. The purpose of this study is to construct a mouse model which can resemble the pathology of UCMD. miR-29 is closely related to extracellular matrix deposition of tissues and organs. To address this issue, we developed a mouse model for overexpression miR-29 using Tet-on system. In the muscle-specific miR-29ab1 cluster transgenic mice model, we found that mice exhibited dyskinesia, dyspnea, and spinal anomaly. The skeletal muscle was damaged and regenerated. At the same time, we clarify the molecular mechanism of the role of miR-29 in this process. Different from human, Col4a1 and Col4a2, target genes of miR-29, are the key pathogenic genes associating with these phenotypes. This mouse model simulates the human clinical and pathological characteristics of UCMD patients and is helpful for the subsequent research and treatment of UCMD.
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- 2019
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29. Melatonin Improves Parthenogenetic Development of Vitrified–Warmed Mouse Oocytes Potentially by Promoting G1/S Cell Cycle Progression
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Bo Pan, Haoxuan Yang, Zhenzheng Wu, Izhar Hyder Qazi, Guoshi Liu, Hongbing Han, Qingyong Meng, and Guangbin Zhou
- Subjects
melatonin ,oocyte vitrification ,redox homeostasis ,cell cycle ,developmental potential ,mouse ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
This study aimed to investigate the effect of melatonin on the cell cycle of parthenogenetic embryos derived from vitrified mouse metaphase II (MII) oocytes. Fresh oocytes were randomly allocated into three groups: untreated (control), or vitrified by the open-pulled straw method without (Vitrification group) or with melatonin (MT) supplementation (Vitrification + MT group). After warming, oocytes were parthenogenetically activated and cultured in vitro, then the percentage of embryos in the G1/S phase, the levels of reactive oxygen species (ROS) and glutathione (GSH), and the mRNA expression of cell cycle-related genes (P53, P21 and E2F1) in zygotes and their subsequent developmental potential in vitro were evaluated. The results showed that the vitrification/warming procedures significantly decreased the frequency of the S phase, markedly increased ROS and GSH levels and the expression of P53 and P21 genes, and decreased E2F1 expression in zygotes at the G1 stage and their subsequent development into 2-cell and blastocyst stage embryos. However, when 10−9 mol/L MT was administered for the whole duration of the experiment, the frequency of the S phase in zygotes was significantly increased, while the other indicators were also significantly improved and almost recovered to the normal levels shown in the control. Thus, MT might promote G1-to-S progression via regulation of ROS, GSH and cell cycle-related genes, potentially increasing the parthenogenetic development ability of vitrified⁻warmed mouse oocytes.
- Published
- 2018
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30. Post-transcriptional Regulation of Keratinocyte Progenitor Cell Expansion, Differentiation and Hair Follicle Regression by miR-22.
- Author
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Shukai Yuan, Feifei Li, Qingyong Meng, Yiqiang Zhao, Lei Chen, Hongquan Zhang, Lixiang Xue, Xiuqing Zhang, Christopher Lengner, and Zhengquan Yu
- Subjects
Genetics ,QH426-470 - Abstract
Hair follicles (HF) undergo precisely regulated recurrent cycles of growth, cessation, and rest. The transitions from anagen (growth), to catagen (regression), to telogen (rest) involve a physiological involution of the HF. This process is likely coordinated by a variety of mechanisms including apoptosis and loss of growth factor signaling. However, the precise molecular mechanisms underlying follicle involution after hair keratinocyte differentiation and hair shaft assembly remain poorly understood. Here we demonstrate that a highly conserved microRNA, miR-22 is markedly upregulated during catagen and peaks in telogen. Using gain- and loss-of-function approaches in vivo, we find that miR-22 overexpression leads to hair loss by promoting anagen-to-catagen transition of the HF, and that deletion of miR-22 delays entry to catagen and accelerates the transition from telogen to anagen. Ectopic activation of miR-22 results in hair loss due to the repression a hair keratinocyte differentiation program and keratinocyte progenitor expansion, as well as promotion of apoptosis. At the molecular level, we demonstrate that miR-22 directly represses numerous transcription factors upstream of phenotypic keratin genes, including Dlx3, Foxn1, and Hoxc13. We conclude that miR-22 is a critical post-transcriptional regulator of the hair cycle and may represent a novel target for therapeutic modulation of hair growth.
- Published
- 2015
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31. Analysis of immunoglobulin transcripts in the ostrich Struthio camelus, a primitive avian species.
- Author
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Tian Huang, Min Zhang, Zhiguo Wei, Ping Wang, Yi Sun, Xiaoxiang Hu, Liming Ren, Qingyong Meng, Ran Zhang, Ying Guo, Lennart Hammarstrom, Ning Li, and Yaofeng Zhao
- Subjects
Medicine ,Science - Abstract
Previous studies on the immunoglobulin (Ig) genes in avian species are limited (mainly to galliformes and anseriformes) but have revealed several interesting features, including the absence of the IgD and Igκ encoding genes, inversion of the IgA encoding gene and the use of gene conversion as the primary mechanism to generate an antibody repertoire. To better understand the Ig genes and their evolutionary development in birds, we analyzed the Ig genes in the ostrich (Struthio camelus), which is one of the most primitive birds. Similar to the chicken and duck, the ostrich expressed only three IgH chain isotypes (IgM, IgA and IgY) and λ light chains. The IgM and IgY constant domains are similar to their counterparts described in other vertebrates. Although conventional IgM, IgA and IgY cDNAs were identified in the ostrich, we also detected a transcript encoding a short membrane-bound form of IgA (lacking the last two C(H) exons) that was undetectable at the protein level. No IgD or κ encoding genes were identified. The presence of a single leader peptide in the expressed heavy chain and light chain V regions indicates that gene conversion also plays a major role in the generation of antibody diversity in the ostrich. Because the ostrich is one of the most primitive living aves, this study suggests that the distinct features of the bird Ig genes appeared very early during the divergence of the avian species and are thus shared by most, if not all, avian species.
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- 2012
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32. Immunoglobulin genomics in the guinea pig (Cavia porcellus).
- Author
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Yongchen Guo, Yonghua Bao, Qingwen Meng, Xiaoxiang Hu, Qingyong Meng, Liming Ren, Ning Li, and Yaofeng Zhao
- Subjects
Medicine ,Science - Abstract
In science, the guinea pig is known as one of the gold standards for modeling human disease. It is especially important as a molecular and cellular biology model for studying the human immune system, as its immunological genes are more similar to human genes than are those of mice. The utility of the guinea pig as a model organism can be further enhanced by further characterization of the genes encoding components of the immune system. Here, we report the genomic organization of the guinea pig immunoglobulin (Ig) heavy and light chain genes. The guinea pig IgH locus is located in genomic scaffolds 54 and 75, and spans approximately 6,480 kb. 507 V(H) segments (94 potentially functional genes and 413 pseudogenes), 41 D(H) segments, six J(H) segments, four constant region genes (μ, γ, ε, and α), and one reverse δ remnant fragment were identified within the two scaffolds. Many V(H) pseudogenes were found within the guinea pig, and likely constituted a potential donor pool for gene conversion during evolution. The Igκ locus mapped to a 4,029 kb region of scaffold 37 and 24 is composed of 349 V(κ) (111 potentially functional genes and 238 pseudogenes), three J(κ) and one C(κ) genes. The Igλ locus spans 1,642 kb in scaffold 4 and consists of 142 V(λ) (58 potentially functional genes and 84 pseudogenes) and 11 J(λ) -C(λ) clusters. Phylogenetic analysis suggested the guinea pig's large germline V(H) gene segments appear to form limited gene families. Therefore, this species may generate antibody diversity via a gene conversion-like mechanism associated with its pseudogene reserves.
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- 2012
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33. Reciprocal communication between FAPs and muscle cells via distinct extracellular vesicle miRNAs in muscle regeneration.
- Author
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Yingying Yu, Yang Su, Guoxiao Wang, Miaomiao Lan, Jin Liu, Martin, Ruben Garcia, Brandao, Bruna Brasil, Lino, Marsel, Lei Li, Chang Liu, Kahn, C. Ronald, and Qingyong Meng
- Subjects
MUSCLE regeneration ,EXTRACELLULAR vesicles ,MUSCLE cells ,MICRORNA ,INTRAMUSCULAR injections - Abstract
Muscle regeneration is a complex process relying on precise teamwork between multiple cell types, including muscle stem cells (MuSCs) and fibroadipogenic progenitors (FAPs). FAPs are also the main source of intramuscular adipose tissue (IMAT). Muscles without FAPs exhibit decreased IMAT infiltration but also deficient muscle regeneration, indicating the importance of FAPs in the repair process. Here, we demonstrate the presence of bidirectional crosstalk between FAPs and MuSCs via their secretion of extracellular vesicles (EVs) containing distinct clusters of miRNAs that is crucial for normal muscle regeneration. Thus, after acute muscle injury, there is activation of FAPs leading to a transient rise in IMAT. These FAPs also release EVs enriched with a selected group of miRNAs, a number of which come from an imprinted region on chromosome 12. The most abundant of these is miR-127-3p, which targets the sphingosine-1-phosphate receptor S1pr3 and activates myogenesis. Indeed, intramuscular injection of EVs from immortalized FAPs speeds regeneration of injured muscle. In late stages of muscle repair, in a feedback loop, MuSCs and their derived myoblasts/myotubes secrete EVs enriched in miR-206-3p and miR-27a/b-3p. The miRNAs repress FAP adipogenesis, allowing full muscle regeneration. Together, the reciprocal communication between FAPs and muscle cells via miRNAs in their secreted EVs plays a critical role in limiting IMAT infiltration while stimulating muscle regeneration, hence providing an important mechanism for skeletal muscle repair and homeostasis. [ABSTRACT FROM AUTHOR]
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- 2024
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34. Melatonin improves the first cleavage of parthenogenetic embryos from vitrified–warmed mouse oocytes potentially by promoting cell cycle progression
- Author
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Jingyu Yang, Qingyong Meng, Hongbing Han, Tianyi Lv, Yan Zhang, Shichao Guo, Jianpeng Qin, Bo Pan, Shengqin Zang, Guang-Bin Zhou, Izhar Hyder Qazi, and Changjun Zeng
- Subjects
0301 basic medicine ,DNA damage ,Veterinary medicine ,Cell cycle ,Cleavage (embryo) ,Biochemistry ,SF1-1100 ,Andrology ,03 medical and health sciences ,0302 clinical medicine ,SF600-1100 ,medicine ,Blastocyst ,Melatonin ,Metaphase II oocyte ,Zygote ,Pronucleus ,Chemistry ,Research ,Embryo ,Vitrification ,Animal culture ,030104 developmental biology ,medicine.anatomical_structure ,Cleavage rate ,Apoptosis ,Parthenogenetic activation ,Animal Science and Zoology ,030217 neurology & neurosurgery ,Food Science ,Biotechnology - Abstract
Background This study investigated the effect of melatonin (MT) on cell cycle (G1/S/G2/M) of parthenogenetic zygotes developed from vitrified-warmed mouse metaphase II (MII) oocytes and elucidated the potential mechanism of MT action in the first cleavage of embryos. Results After vitrification and warming, oocytes were parthenogenetically activated (PA) and in vitro cultured (IVC). Then the spindle morphology and chromosome segregation in oocytes, the maternal mRNA levels of genes including Miss, Doc1r, Setd2 and Ythdf2 in activated oocytes, pronuclear formation, the S phase duration in zygotes, mitochondrial function at G1 phase, reactive oxygen species (ROS) level at S phase, DNA damage at G2 phase, early apoptosis in 2-cell embryos, cleavage and blastocyst formation rates were evaluated. The results indicated that the vitrification/warming procedures led to following perturbations 1) spindle abnormalities and chromosome misalignment, alteration of maternal mRNAs and delay in pronucleus formation, 2) decreased mitochondrial membrane potential (MMP) and lower adenosine triphosphate (ATP) levels, increased ROS production and DNA damage, G1/S and S/G2 phase transition delay, and delayed first cleavage, and 3) increased early apoptosis and lower levels of cleavage and blastocyst formation. Our results further revealed that such negative impacts of oocyte cryopreservation could be alleviated by supplementation of warming, recovery, PA and IVC media with 10− 9 mol/L MT before the embryos moved into the 2-cell stage of development. Conclusions MT might promote cell cycle progression via regulation of MMP, ATP, ROS and maternal mRNA levels, potentially increasing the first cleavage of parthenogenetic zygotes developed from vitrified–warmed mouse oocytes and their subsequent development.
- Published
- 2021
35. Inactivating IL34 promotes regenerating muscle stem cell expansion and attenuates Duchenne muscular dystrophy in mouse models.
- Author
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Yang Su, Yuxin Cao, Chang Liu, Qing Xu, Na Li, Miaomiao Lan, Lei Li, Kun Wang, Zeyu Zhang, and Qingyong Meng
- Published
- 2023
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36. Selection and validation of suitable reference genes in adipose tissue of Jianzhou Da'er Goat (Capra hircus)
- Author
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Yong Wang, Guangjie Xie, Jiangjiang Zhu, Qing Xu, Lu Tang, Qingyong Meng, Mingfeng Jiang, and Yaqiu Lin
- Subjects
General Veterinary ,040301 veterinary sciences ,Veterinary medicine ,goat ,0402 animal and dairy science ,Adipose tissue ,reference genes ,04 agricultural and veterinary sciences ,Biology ,040201 dairy & animal science ,Molecular biology ,Reverse transcriptase ,adipose tissue ,0403 veterinary science ,Mrna level ,Reference genes ,SF600-1100 ,Capra hircus ,Animal Science and Zoology ,Gene ,Selection (genetic algorithm) ,gene expression stability - Abstract
Reverse transcription quantitative real-time PCR (RT-qPCR) is a common and high-efficiency technique for detecting the mRNA levels of genes where suitable reference genes were introduced to normalize the data of RT-qPCR. However, little is known about the suitable reference genes in the adipose tissue of Jianzhou da’er goat. Here, six housekeeping genes, including the ACTB, ALAS1, EIF3K, HSP90, RPLP0, and UXT, were selected as candidate reference genes, while the FASN, HSL, LPL, and PID1 were used as the target genes for the validation of the suitability of identified reference genes. After detecting the expression levels of selected genes, the geNorm, NormFinder, and RefFinder softwares were applied to analyze the obtained data of candidate reference genes. The suitability analysis showed that the HSP90 was the most stable candidate reference gene, followed by the ALAS1 and RPLP0 gene. The validation experiment not only verified that the HSP90 was more stable than ACTB as the reference gene, but also confirmed that the HSP90 alone and a combination of HSP90, ALAS1, and RPLP0 could play the same roles in the normalization of target genes. Therefore, we strongly suggested that the HSP90 alone and the combination of three genes (HSP90, ALAS1, and RPLP0) could be used as the suitable reference for normalizing gene expression data in adipose tissue of Jianzhou da’er goat.
- Published
- 2021
37. Significant Influence of Welding Heat Input on the Microstructural Characteristics and Mechanical Properties of the Simulated CGHAZ in High Nitrogen V-Alloyed Steel
- Author
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Ruifen Wang, Guoping Luo, Qingyong Meng, Jing Zhang, and Wenbin Xin
- Subjects
Technology ,Materials science ,0211 other engineering and technologies ,Chemicals: Manufacture, use, etc ,02 engineering and technology ,Welding ,TP1-1185 ,law.invention ,welding heat input ,law ,microstructural characteristics ,impact toughness ,High nitrogen ,General Materials Science ,Physical and Theoretical Chemistry ,021102 mining & metallurgy ,Impact toughness ,Chemical technology ,Metallurgy ,TP200-248 ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,Mechanics of Materials ,0210 nano-technology ,simulated cghaz - Abstract
The microstructural characteristics and mechanical properties of the simulated coarse grained heat affected zone (CGHAZ) in high N V-alloyed steel have been conducted under different welding heat input, characterized by the cooling time taken from 800°C to 500°C (t8/5). The experimental results show that the microstructure is dominantly composed of lath bainite (LB) and granular bainite (GB) at t8/5 30 s– 90 s. The content of LB decreases with t8/5 increasing, and that of GB increases. When t8/5 further increases to 120 s and 180 s, the microstructure mainly consists of intragranular polygonal ferrite (IPF) and acicular ferrite (IAF). The higher t8/5 leads to the increased content of intragranular ferrite (IGF). Meanwhile, the prior austenite grain size (PAGS) progressively increases from 56 ± 6.0 μm to 148 ± 9.9 μm as t8/5 increases from 30 s to 180 s. Besides, EBSD analysis indicates that the fraction of high angle grain boundaries (HAGBs) is 0.570, 0.427 and 0.624, respectively, corresponding to t8/5 30, 90 and 180 s. Moreover, the impact toughness decreases as t8/5 increases from 30 s to 90 s caused by the increased PAGS and GB content, and then sharply increases with t8/5 exceeding 90 s due to the increased formation of IGF, especially IAF. Furthermore, the high nitrogen content accelerates V(C,N) precipitation, which not only inhibits the coarsening of prior austenite grains, but promotes the formation of IGF, resulting in the increased number of HAGBs and raising impact toughness.
- Published
- 2020
38. Ectopic Expression of VvSUC27 Induces Stenospermocarpy and Sugar Accumulation in Tomato Fruits
- Author
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Zhefang Deng, Jing Yan, Wenrui Tu, Ling Yin, Han Gao, Jinxu Xu, Nan Zhang, Yali Zhang, Qingyong Meng, Lei Zhu, Jie Wang, Yumeng Cai, and Wenjie Dong
- Subjects
chemistry.chemical_classification ,Sucrose ,fungi ,stenospermocarpy ,food and beverages ,Plant culture ,Plant Science ,Carbohydrate metabolism ,Biology ,tomato ,SB1-1110 ,Horticulture ,chemistry.chemical_compound ,chemistry ,Anthesis ,Auxin ,Vitis vinifera ,Stenospermocarpy ,VvSUC ,Petal ,Ectopic expression ,Sugar ,transcriptomic analysis ,overexpression - Abstract
Seedless fruits are favorable in the market because of their ease of manipulation. Sucrose transporters (SUTs or SUCs) are essential for carbohydrate metabolism in plants. Whether SUTs participate directly in causing stenospermocarpy, thereby increasing fruit quality, remains unclear. Three SUTs, namely, VvSUC11, VvSUC12, and VvSUC27 from Vitis vinifera, were characterized and ectopic expression in tomatoes. VvSUC11- and VvSUC12-overexpressing lines had similar flower and fruit phenotypes compared with those of the wild type. VvSUC27-overexpressing lines produced longer petals and pistils, an abnormal stigma, much less and shrunken pollen, and firmer seedless fruits. Moreover, produced fruits from all VvSUC-overexpressing lines had a higher soluble solid content and sugar concentration. Transcriptomic analysis revealed more genes associated with carbohydrate metabolism and sugar transport and showed downregulation of auxin- and ethylene-related signaling pathways during early fruit development in VvSUC27-overexpressing lines relative to that of the wild type. Our findings demonstrated that stenospermocarpy can be induced by overexpression of VvSUC27 through a consequential reduction in nutrient delivery to pollen at anthesis, with a subsequent downregulation of the genes involved in carbohydrate metabolism and hormone signaling. These commercially desirable results provide a new strategy for bioengineering stenospermocarpy in tomatoes and in other fruit plants.
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- 2021
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39. Hetero/Homo-Complexes of Sucrose Transporters May Be a Subtle Mode to Regulate Sucrose Transportation in Grape Berries
- Author
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Jing Yan, Yumeng Cai, Han Gao, Qingyong Meng, Wenrui Tu, Ling Yin, Wenjie Dong, Yali Zhang, Zhefang Deng, Jie Wang, and Jinxu Xu
- Subjects
Sucrose ,QH301-705.5 ,functional variation ,different varieties ,functional regulation ,Article ,Catalysis ,oligomerization ,Inorganic Chemistry ,Structure-Activity Relationship ,chemistry.chemical_compound ,sucrose transporter ,Vitis ,Biology (General) ,Physical and Theoretical Chemistry ,Sugar ,QD1-999 ,Molecular Biology ,Spectroscopy ,Plant Proteins ,Chemistry ,Organic Chemistry ,Membrane Transport Proteins ,food and beverages ,Biological Transport ,Transporter ,General Medicine ,Sucrose transport ,Yeast ,Computer Science Applications ,Membrane protein ,Biochemistry ,Fruit ,Phloem ,Protein Multimerization ,Function (biology) - Abstract
The sugar distribution mechanism in fruits has been the focus of research worldwide, however, it remains unclear. In order to elucidate the relevant mechanisms in grape berries, the expression, localization, function, and regulation of three sucrose transporters were studied in three representative Vitis varieties. Both SUC11 and SUC12 expression levels were positively correlated with sugar accumulation in grape berries, whereas SUC27 showed a negative relationship. The alignment analysis and sucrose transport ability of isolated SUCs were determined to reflect coding region variations among V. vinifera, V. amurensis Ruper, and V. riparia, indicating that functional variation existed in one SUT from different varieties. Furthermore, potentially oligomerized abilities of VvSUCs colocalized in the sieve elements of the phloem as plasma membrane proteins were verified. The effects of oligomerization on transport properties were characterized in yeast. VvSUC11 and VvSUC12 are high-affinity/low-capacity types of SUTs that stimulate each other by upregulating Vmax and Km, inhibiting sucrose transport, and downregulating the Km of VvSUC27. Thus, changes in the distribution of different SUTs in the same cell govern functional regulation. The activation and inhibition of sucrose transport could be achieved in different stages and tissues of grape development to achieve an effective distribution of sugar.
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- 2021
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40. A study of natural IgG antibodies against ATP-binding cassette subfamily C member 3 in oral squamous cell carcinoma
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Xiu Liu, Ying Hu, Xiaoyu Wang, Zhicheng Huang, Qingyong Meng, and Ziyi He
- Subjects
0301 basic medicine ,ATP-binding cassette subfamily C member 3 ,Apoptosis ,lcsh:RC254-282 ,Flow cytometry ,03 medical and health sciences ,0302 clinical medicine ,Biomarkers, Tumor ,Tumor Cells, Cultured ,medicine ,Humans ,natural antibody ,Radiology, Nuclear Medicine and imaging ,oral squamous cell carcinoma cells ,Autoantibodies ,Cell Proliferation ,medicine.diagnostic_test ,biology ,Chemistry ,Cell growth ,Cell Cycle ,General Medicine ,Cell cycle ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Molecular biology ,Reverse transcriptase ,tumor immunity ,030104 developmental biology ,Oncology ,Cell culture ,Immunoglobulin G ,030220 oncology & carcinogenesis ,ABCC3 ,Carcinoma, Squamous Cell ,biology.protein ,Mouth Neoplasms ,Multidrug Resistance-Associated Proteins ,Antibody - Abstract
Aims: ATP-binding cassette subfamily C member 3 (ABCC3) is involved in multidrug resistance and is overexpressed in some solid tumors. Recent work revealed an increase in circulating anti-ABCC3 antibodies in lung and esophageal cancers. This in vitro study was undertaken to investigate the effects of the natural IgG antibody against the ABCC3-derived peptide antigen on proliferation of oral squamous cell carcinoma (OSCC) cells and augment the development of efficient and effective treatments in patients with OSCC. Subjects and Methods: An in-house enzyme-linked immunosorbent assay was applied to detect anti-ABCC3 IgG antibody in human plasma. Two OSCC cell lines, CAL27 and SCC15, were cultured with 20% plasma either positive or negative for anti-ABCC3 IgG. Cell proliferation was quantified by the CCK-8 method, and cell apoptosis and cell cycle distribution were analyzed by flow cytometry. The expression of the ABCC3 gene in the cell lines was analyzed by reverse transcriptase quantitative real-time polymerase chain reaction. Results: The results showed that plasma anti-ABCC3 IgG significantly inhibited the proliferation of CAL27 cells but not SCC15 cells, although ABCC3 was expressed in both cell lines. The proportion of apoptotic cells was significantly higher in CAL27 cells treated with anti-ABCC3 IgG-positive plasma than in those treated with IgG-negative plasma. Cell cycle progression was arrested in CAL27 cells treated with anti-ABCC3 IgG-positive plasma. Conclusions: Our data suggest that human plasma anti-ABCC3 IgG may be a promising agent in anti-OSCC therapy, although further studies are needed to arrive at a definitive conclusion.
- Published
- 2019
41. Islr regulates canonical Wnt signaling-mediated skeletal muscle regeneration by stabilizing Dishevelled-2 and preventing autophagy
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Chuncheng Liu, Zhengquan Yu, Yuying Zhang, Ning Li, Lei Li, Yang Su, Qiuyan Li, Fudi Wang, Meng Wang, Kuo Zhang, Yingying Yu, Miaomiao Lan, Lijie Gu, Mengxu Ge, Chang Liu, Yaofeng Zhao, Tongtong Wang, and Qingyong Meng
- Subjects
0301 basic medicine ,Science ,Dishevelled Proteins ,Immunoglobulins ,General Physics and Astronomy ,Mice, Transgenic ,Biology ,Article ,General Biochemistry, Genetics and Molecular Biology ,Cell Line ,Myoblasts ,03 medical and health sciences ,Autophagy ,medicine ,Animals ,Humans ,Regeneration ,Myocyte ,Muscle, Skeletal ,lcsh:Science ,Wnt Signaling Pathway ,Cells, Cultured ,Myogenin ,Mice, Knockout ,chemistry.chemical_classification ,Multidisciplinary ,Myogenesis ,Regeneration (biology) ,Wnt signaling pathway ,Skeletal muscle ,Cell Differentiation ,General Chemistry ,Cell biology ,Dishevelled ,HEK293 Cells ,030104 developmental biology ,medicine.anatomical_structure ,chemistry ,RNA Interference ,lcsh:Q - Abstract
Satellite cells are crucial for skeletal muscle regeneration, but the molecular mechanisms regulating satellite cells are not entirely understood. Here, we show that the immunoglobulin superfamily containing leucine-rich repeat (Islr), a newly identified marker for mesenchymal stem cells, stabilizes canonical Wnt signaling and promote skeletal muscle regeneration. Loss of Islr delays skeletal muscle regeneration in adult mice. In the absence of Islr, myoblasts fail to develop into mature myotubes due to defective differentiation. Islr interacts with Dishevelled-2 (Dvl2) to activate canonical Wnt signaling, consequently regulating the myogenic factor myogenin (MyoG). Furthermore, Islr stabilizes Dvl2 by reducing the level of LC3-labeled Dvl2 and preventing cells from undergoing autophagy. Together, our findings identify Islr as an important regulator for skeletal muscle regeneration., “Satellite cells are crucial for skeletal muscle regeneration. Here the authors show that immunoglobulin superfamily containing leucine-rich repeat (Islr) promotes skeletal muscle regeneration via a mechanism involving Dishevelled-2 stabilization in satellite cells and protection from autophagy.
- Published
- 2018
42. Secreted stromal protein ISLR promotes intestinal regeneration by suppressing epithelial Hippo signaling
- Author
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Cong Lv, Yongli Song, Xiaole Sheng, Zhanju Liu, Maksim V. Plikus, Qingyong Meng, Sujuan Du, Yingzi Cong, Jiuzhi Xu, Yongting Luo, Yang Tang, Yanmei Chen, Yuhua Tian, Min Deng, Zhengquan Yu, Bing Zhang, Pengbo Lou, Yuwei Pan, Yuan Li, Guili Li, and Zhaocai Zhou
- Subjects
Male ,Stromal cell ,Immunoglobulins ,Biology ,Protein Serine-Threonine Kinases ,medicine.disease_cause ,General Biochemistry, Genetics and Molecular Biology ,Proto-Oncogene Protein c-ets-1 ,03 medical and health sciences ,Gene Knockout Techniques ,Mice ,0302 clinical medicine ,ETS1 ,medicine ,Animals ,Humans ,Hippo Signaling Pathway ,Intestinal Mucosa ,Promoter Regions, Genetic ,Molecular Biology ,Transcription factor ,030304 developmental biology ,0303 health sciences ,General Immunology and Microbiology ,General Neuroscience ,Regeneration (biology) ,Articles ,HCT116 Cells ,Inflammatory Bowel Diseases ,Epithelium ,Cell biology ,Disease Models, Animal ,medicine.anatomical_structure ,HEK293 Cells ,Hippo signaling ,Mutation ,Signal transduction ,Carcinogenesis ,Colorectal Neoplasms ,HT29 Cells ,030217 neurology & neurosurgery ,Signal Transduction - Abstract
The Hippo-YAP signaling pathway plays an essential role in epithelial cells during intestinal regeneration and tumorigenesis. However, the molecular mechanism linking stromal signals to YAP-mediated intestinal regeneration and tumorigenesis is poorly defined. Here, we report a stroma-epithelium ISLR-YAP signaling axis essential for stromal cells to modulate epithelial cell growth during intestinal regeneration and tumorigenesis. Specifically, upon inflammation and in cancer, an oncogenic transcription factor ETS1 in stromal cells induces expression of a secreted protein ISLR that can inhibit Hippo signaling and activate YAP in epithelial cells. Deletion of Islr in stromal cells in mice markedly impaired intestinal regeneration and suppressed tumorigenesis in the colon. Moreover, the expression of stromal cell-specific ISLR and ETS1 significantly increased in inflamed mucosa of human IBD patients and in human colorectal adenocarcinoma, accounting for the epithelial YAP hyperactivation. Collectively, our findings provide new insights into the signaling crosstalk between stroma and epithelium during tissue regeneration and tumorigenesis.
- Published
- 2020
43. Incorporation of a skeletal muscle-specific enhancer in the regulatory region of Igf1 upregulates IGF1 expression and induces skeletal muscle hypertrophy
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Yanjun Dong, Ning Li, Yaofeng Zhao, Qingyong Meng, and Yunlong Zou
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Male ,0301 basic medicine ,Myosin Light Chains ,Myosin light-chain kinase ,Heart malformation ,Science ,Muscle Fibers, Skeletal ,Biology ,Article ,Muscle hypertrophy ,Animals, Genetically Modified ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Muscular Diseases ,Gene expression ,medicine ,Animals ,Insulin-Like Growth Factor I ,Muscle, Skeletal ,Enhancer ,Multidisciplinary ,Myogenesis ,Skeletal muscle ,Hypertrophy ,Up-Regulation ,Cell biology ,Disease Models, Animal ,Enhancer Elements, Genetic ,Phenotype ,030104 developmental biology ,medicine.anatomical_structure ,Medicine ,Female ,C2C12 ,030217 neurology & neurosurgery - Abstract
In this study, we upregulated insulin-like growth factor-1 (IGF1) expression specifically in skeletal muscle by engineering an enhancer into its non-coding regions and verified the expected phenotype in a mouse model. To select an appropriate site for introducing a skeletal muscle-specific myosin light chain (MLC) enhancer, three candidate sites that exhibited the least evolutionary conservation were chosen and validated in C2C12 single-cell colonies harbouring the MLC enhancer at each site. IGF1 was dramatically upregulated in only the site 2 single-cell colony series, and it exhibited functional activity leading to the formation of extra myotubes. Therefore, we chose site 2 to generate a genetically modified (GM) mouse model with the MLC enhancer incorporated by CRISPR/Cas9 technology. The GM mice exhibited dramatically elevated IGF1 levels, which stimulated downstream pathways in skeletal muscle. Female GM mice exhibited more conspicuous muscle hypertrophy than male GM mice. The GM mice possessed similar circulating IGF1 levels and tibia length as their WT littermates; they also did not exhibit heart abnormalities. Our findings demonstrate that genetically modifying a non-coding region is a feasible method to upregulate gene expression and obtain animals with desirable traits.
- Published
- 2018
44. Detection of circulating natural antibodies to inflammatory cytokines in type-2 diabetes and clinical significance
- Author
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Cailing Qiu, Xuan Zhang, Weiyi Cai, Xiangyun Chen, Jun Wei, Hongyu Zhang, and Qingyong Meng
- Subjects
0301 basic medicine ,medicine.medical_specialty ,Clinical Biochemistry ,Type 2 diabetes ,Proinflammatory cytokine ,03 medical and health sciences ,chemistry.chemical_compound ,Insulin resistance ,Antigen ,Internal medicine ,IgG antibody ,medicine ,Interleukin 8 ,Type-2 diabetes ,biology ,business.industry ,Research ,lcsh:RM1-950 ,Cell Biology ,medicine.disease ,Inflammatory cytokines ,030104 developmental biology ,Endocrinology ,lcsh:Therapeutics. Pharmacology ,chemistry ,Immunology ,Natural antibodies ,biology.protein ,Tumor necrosis factor alpha ,ELISA ,Glycated hemoglobin ,Antibody ,business - Abstract
Background Inflammatory cytokines have been demonstrated to be involved in developing insulin resistance and type-2 diabetes (T2D). Natural antibodies in the circulation have protective effects on common diseases in humans. The present study was thus designed to test the hypothesis that natural antibodies against inflammatory cytokines could be associated with T2D. Methods An enzyme-linked immunosorbent assay (ELISA) was developed in-house to detect plasma IgG against peptide antigens derived from interleukin 1α (IL1α), IL1β, IL6, IL8 and tumor necrosis factor-α (TNF-α) in 200 patients with T2D and 220 control subjects. Results Binary regression showed that compared with control subjects, T2D patients had a decreased level of plasma anti-IL6 IgG (adjusted r 2=0.034, p=0.0001), anti-IL8 IgG (adjusted r 2=0.021, p=0.002) and anti-TNF-α IgG (adjusted r 2=0.017, p=0.003). Female patients mainly contributed to decreased levels of anti-IL6 IgG (adjusted r 2=0.065, p=0.0008) and anti-IL8 IgG (adjusted r 2=0.056, p=0.003), while male patients mainly contributed to decreased anti-TNF-α IgG levels (adjusted r 2=0.024, p=0.005). ROC curve analysis revealed a sensitivity of 16.5% against specificity of 95.5% for anti-IL6 IgG assay and a sensitivity of 19.5% against specificity of 95.9% for anti-IL8 IgG assay. Glycated hemoglobin levels measured after 6-month glucose-lowering treatment appeared to be inversely correlated with plasma anti-IL1α IgG (r=-0.477, df=17, p=0.039) and anti-IL6 IgG (r=-0.519, df=17, p=0.023) although such correlation failed to survive the Bonferroni correction. Conclusions Deficiency of natural IgG against inflammatory cytokines is likely to be a risk factor for T2D development and detection of such antibodies may be useful for personalized treatment of the disease. Electronic supplementary material The online version of this article (10.1186/s12950-017-0171-6) contains supplementary material, which is available to authorized users.
- Published
- 2017
45. MiR-31 promotes mammary stem cell expansion and breast tumorigenesis by suppressing Wnt signaling antagonists
- Author
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Yue Zhang, Baowei Jiao, Sarah E. Millar, Jianwei Shuai, Fengyin Li, Cong Lv, Xing Dai, Fazheng Ren, Shukai Yuan, Qingyong Meng, Xiaole Sheng, Xu Feng, Mingang Xu, Yongli Song, Yuhua Tian, Xiang Li, Liming Luan, Maksim V. Plikus, Thomas Andl, Christopher J. Lengner, Wei Cui, and Zhengquan Yu
- Subjects
0301 basic medicine ,METASTASIS SUPPRESSOR ,NF-KAPPA-B ,TO-MESENCHYMAL TRANSITION ,INVASION ,General Physics and Astronomy ,medicine.disease_cause ,Mice ,Cell Self Renewal ,lcsh:Science ,Wnt Signaling Pathway ,beta Catenin ,GENE-EXPRESSION ,Mammary tumor ,education.field_of_study ,Multidisciplinary ,Stem Cells ,NF-kappa B ,Wnt signaling pathway ,EPITHELIAL-CELLS ,CANCER ,3. Good health ,Multidisciplinary Sciences ,Gene Expression Regulation, Neoplastic ,mir-31 ,DIFFERENTIATION ,Neoplastic Stem Cells ,Science & Technology - Other Topics ,Female ,Stem cell ,medicine.medical_specialty ,Science ,Population ,Down-Regulation ,Breast Neoplasms ,Mice, Transgenic ,Biology ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,Cancer stem cell ,Cell Line, Tumor ,Internal medicine ,GLAND DEVELOPMENT ,medicine ,Animals ,Humans ,Mammary Glands, Human ,education ,Cell Proliferation ,P-CADHERIN ,Science & Technology ,General Chemistry ,Mice, Inbred C57BL ,Wnt Proteins ,MicroRNAs ,030104 developmental biology ,Endocrinology ,DKK1 ,Cancer research ,lcsh:Q ,Carcinogenesis - Abstract
MicroRNA-mediated post-transcriptional regulation plays key roles in stem cell self-renewal and tumorigenesis. However, the in vivo functions of specific microRNAs in controlling mammary stem cell (MaSC) activity and breast cancer formation remain poorly understood. Here we show that miR-31 is highly expressed in MaSC-enriched mammary basal cell population and in mammary tumors, and is regulated by NF-κB signaling. We demonstrate that miR-31 promotes mammary epithelial proliferation and MaSC expansion at the expense of differentiation in vivo. Loss of miR-31 compromises mammary tumor growth, reduces the number of cancer stem cells, as well as decreases tumor-initiating ability and metastasis to the lung, supporting its pro-oncogenic function. MiR-31 modulates multiple signaling pathways, including Prlr/Stat5, TGFβ and Wnt/β-catenin. Particularly, it activates Wnt/β-catenin signaling by directly targeting Wnt antagonists, including Dkk1. Importantly, Dkk1 overexpression partially rescues miR31-induced mammary defects. Together, these findings identify miR-31 as the key regulator of MaSC activity and breast tumorigenesis.
- Published
- 2017
46. Lattice effects of surface cell: Multilayer multiconfiguration time-dependent Hartree study on surface scattering of CO/Cu(100).
- Author
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Qingyong Meng and Hans-Dieter Meyer
- Subjects
- *
SURFACE scattering , *HARTREE-Fock approximation , *POTENTIAL energy surfaces , *HAMILTONIAN systems , *VIBRATIONAL redistribution (Molecular physics) - Abstract
To study the scattering of CO off a movable Cu(100) surface, extensive multilayer multiconfiguration time-dependent Hartree (ML-MCTDH) calculations are performed based on the SAP [R. Marquardt et al., J. Chem. Phys. 132, 074108 (2010)] potential energy surface in conjunction with a recently developed expansion model [Q. Meng and H.-D. Meyer, J. Chem. Phys. 143, 164310 (2015)] for including lattice motion. The surface vibration potential is constructed by a sum of Morse potentials where the parameters are determined by simulating the vibrational energies of a clean Cu(100) surface. Having constructed the total Hamiltonian, extensive dynamical calculations in both timeindependent and time-dependent schemes are performed. Two-layer MCTDH (i.e., normal MCTDH) block-improved-relaxations (time-independent scheme) show that increasing the number of included surface vibrational dimensions lets the vibrational energies of CO/Cu(100) decrease for the frustrated translation (T mode), which is of low energy but increase those of the frustrated rotation (R mode) and the CO-Cu stretch (S mode), whose vibrational energies are larger than the energies of the in-plane surface vibrations (~79 cm-1). This energy-shifting behavior was predicted and discussed by a simple model in our previous publication [Q. Meng and H.-D. Meyer, J. Chem. Phys. 143, 164310 (2015)]. By the flux analysis of the MCTDH/ML-MCTDH propagated wave packets, we calculated the sticking probabilities for the X + 0D, X + 1D, X + 3D, X + 5D, and X + 15D systems, where "X" stands for the used dimensionality of the CO/rigid-surface system and the second entry denotes the number of surface degrees of freedom included. From these sticking probabilities, the X + 5D/15D calculations predict a slower decrease of sticking with increasing energy as compared to the sticking of the X + 0D/1D/3D calculations. This is because the translational energy of CO is more easily transferred to surface vibrations, when the vibrational dimensionality of the surface is enlarged. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
47. Ring-polymer molecular dynamics studies on the rate coefficient of the abstraction channel of hydrogen plus ethane, propane, and dimethyl ether.
- Author
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Qingyong Meng and Jun Chen
- Subjects
- *
METHYL ether , *MOLECULAR physics , *MOLECULAR interactions , *ALIPHATIC hydrocarbons , *HYDROGEN-deuterium exchange - Abstract
To accurately compute the rates of the abstraction channels of hydrogen plus ethane (Et), propane (Pr), and dimethyl ether (DME), ring-polymer molecular dynamics (RPMD) method is used in conjunction with the recently constructed local permutation invariant polynomial neural-networks potential energy surface of the parent H + CH4 system [Q. Meng et al., J. Chem. Phys. 144, 154312 (2016)]. For H + Et, one of the H atoms in CH4 of the parent system is replaced by a methyl group, while for the H + DME reaction, it is replaced by the methoxyl group. For the H + Pr reaction, replacing one of the H atoms in CH4 by an ethyl group, the terminal channel is built, meanwhile the middle channel is considered through replacing two H atoms in CH4 by two methyl groups. Since the potential energy barriers of the title reactions must differ from the H + CH4 barrier, the corrections have to be made by computing the ratio of free-energy barriers between H + CH4 and the title reactions at coupled cluster with a full treatment singles and doubles (where the triples contribution is calculated by perturbation theory, that is, CCSD(T)) level. Comparing the present RPMD rates with the previous theoretical and experimental results, good agreement can be found. Moreover, probable reasons for the deviation between the present RPMD rates and the previous experimental ones are discussed. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
48. Role of Selenium and Selenoproteins in Male Reproductive Function: A Review of Past and Present Evidences
- Author
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Zhenzheng Wu, Zhang Ming, Haoxuan Yang, Changjun Zeng, Evangelos Zoidis, Izhar Hyder Qazi, Guang-Bin Zhou, Hongbing Han, Bo Pan, Qingyong Meng, and Christiana Angel
- Subjects
0301 basic medicine ,Future studies ,Physiology ,media_common.quotation_subject ,Clinical Biochemistry ,Context (language use) ,Fertility ,mammalian reproduction ,Review ,Biology ,GPX4 ,Biochemistry ,male fertility ,Mammalian reproduction ,03 medical and health sciences ,selenium ,Molecular Biology ,media_common ,Genetics ,integumentary system ,Selenoprotein P ,lcsh:RM1-950 ,0402 animal and dairy science ,04 agricultural and veterinary sciences ,Cell Biology ,Male reproductive function ,040201 dairy & animal science ,spermatogenesis ,030104 developmental biology ,lcsh:Therapeutics. Pharmacology ,Male fertility ,selenoproteins - Abstract
Selenium (Se) is an important trace mineral having many essential roles at the cellular and organismal levels in animal and human health. The biological effects of Se are mainly carried out by selenoproteins (encoded by 25 genes in humans and 24 in mice). As an essential component of selenoproteins, Se performs structural and enzymic roles; in the latter context it is well known for its catalytic and antioxidative functions. Studies involving different animal models have added great value to our understanding regarding the potential implications of Se and selenoproteins in mammalian fertility and reproduction. In this review, we highlight the implications of selenoproteins in male fertility and reproduction followed by the characteristic biological functions of Se and selenoproteins associated with overall male reproductive function. It is evident from observations of past studies (both animal and human) that Se is essentially required for spermatogenesis and male fertility, presumably because of its vital role in modulation of antioxidant defense mechanisms and other essential biological pathways and redox sensitive transcription factors. However, bearing in mind the evidences from mainstream literature, it is also advisable to perform more studies focusing on the elucidation of additional roles played by the peculiar and canonical selenoproteins i.e., glutathione peroxidase 4 (GPX4) and selenoprotein P (SELENOP) in the male reproductive functions. Nevertheless, search for the elucidation of additional putative mechanisms potentially modulated by other biologically relevant selenoproteins should also be included in the scope of future studies. However, as for the implication of Se in fertility and reproduction in men, though a few clinical trials explore the effects of Se supplementation on male fertility, due to inconsistencies in the recruitment of subjects and heterogeneity of designs, the comparison of such studies is still complicated and less clear. Therefore, further research focused on the roles of Se and selenoproteins is awaited for validating the evidences at hand and outlining any therapeutic schemes intended for improving male fertility. As such, new dimensions could be added to the subject of male fertility and Se supplementation.
- Published
- 2019
49. Fate decision of satellite cell differentiation and self-renewal by miR-31-IL34 axis
- Author
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Ning Li, Ting He, Zhengquan Yu, Min Li, Yuying Zhang, Lei Li, Fan Liu, Chuncheng Liu, Mengxu Ge, Yaofeng Zhao, Jianyun Shi, Yingying Yu, Chang Liu, Miaomiao Lan, Lei Xiong, Kun Wang, Yang Su, Yongli Song, Qingyong Meng, and Tongtong Wang
- Subjects
0301 basic medicine ,STAT3 Transcription Factor ,Satellite Cells, Skeletal Muscle ,Cellular differentiation ,Cell fate determination ,Biology ,Muscle Development ,Article ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Myocyte ,Animals ,Regeneration ,Cell Lineage ,Cell Self Renewal ,Molecular Biology ,Cells, Cultured ,Cell Proliferation ,Janus Kinases ,Mice, Knockout ,Base Sequence ,Interleukins ,Cell Cycle ,Skeletal muscle ,Interleukin ,PAX7 Transcription Factor ,Cell Differentiation ,Cell Biology ,Cell biology ,Mice, Inbred C57BL ,MicroRNAs ,030104 developmental biology ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Knockout mouse ,Interleukin 34 ,Stem cell ,Gene Deletion ,Signal Transduction - Abstract
Quiescent satellite cells (SCs) that are activated to produce numerous myoblasts underpin the complete healing of damaged skeletal muscle. How cell-autonomous regulatory mechanisms modulate the balance among cells committed to differentiation and those committed to self-renewal to maintain the stem cell pool remains poorly explored. Here, we show that miR-31 inactivation compromises muscle regeneration in adult mice by impairing the expansion of myoblasts. miR-31 is pivotal for SC proliferation, and its deletion promotes asymmetric cell fate segregation of proliferating cells, resulting in enhanced myogenic commitment and re-entry into quiescence. Further analysis revealed that miR-31 posttranscriptionally suppresses interleukin 34 (IL34) mRNA, the protein product of which activates JAK–STAT3 signaling required for myogenic progression. IL34 inhibition rescues the regenerative deficiency of miR-31 knockout mice. Our results provide evidence that targeting miR-31 or IL34 activities in SCs could be used to counteract the functional exhaustion of SCs in pathological conditions.
- Published
- 2019
50. Ring polymer molecular dynamics fast computation of rate coefficients on accurate potential energy surfaces in local configuration space: Application to the abstraction of hydrogen from methane.
- Author
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Qingyong Meng, Jun Chen, and Zhang, Dong H.
- Subjects
- *
MOLECULAR dynamics , *POLYMERS , *ARTIFICIAL neural networks , *RATE coefficients (Chemistry) , *POTENTIAL energy surfaces , *HYDROGEN , *METHANE - Abstract
To fast and accurately compute rate coefficients of the H/D + CH4→H2/HD + CH3 reactions, we propose a segmented strategy for fitting suitable potential energy surface (PES), on which ring-polymer molecular dynamics (RPMD) simulations are performed. On the basis of recently developed permutation invariant polynomial neural-network approach [J. Li et al., J. Chem. Phys. 142, 204302 (2015)], PESs in local configuration spaces are constructed. In this strategy, global PES is divided into three parts, including asymptotic, intermediate, and interaction parts, along the reaction coordinate. Since less fitting parameters are involved in the local PESs, the computational efficiency for operating the PES routine is largely enhanced by a factor of ~20, comparing with that for global PES. On interaction part, the RPMD computational time for the transmission coefficient can be further efficiently reduced by cutting off the redundant part of the child trajectories. For H + CH4, good agreements among the present RPMD rates and those from previous simulations as well as experimental results are found. For D + CH4, on the other hand, qualitative agreement between present RPMD and experimental results is predicted. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
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