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Abstract Background The pathophysiology of migraine remains poorly understood, yet a growing number of studies have shown structural connectivity disruptions across large-scale brain networks. Although both structural and functional changes have been found in the cerebellum of migraine patients, the cerebellum has barely been assessed in previous structural connectivity studies of migraine. Our objective is to investigate the structural connectivity of the entire brain, including the cerebellum, in individuals diagnosed with episodic migraine without aura during the interictal phase, compared with healthy controls. Methods To that end, 14 migraine patients and 15 healthy controls were recruited (all female), and diffusion-weighted and T1-weighted MRI data were acquired. The structural connectome was estimated for each participant based on two different whole-brain parcellations, including cortical and subcortical regions as well as the cerebellum. The structural connectivity patterns, as well as global and local graph theory metrics, were compared between patients and controls, for each of the two parcellations, using network-based statistics and a generalized linear model (GLM), respectively. We also compared the number of connectome streamlines within specific white matter tracts using a GLM. Results We found increased structural connectivity in migraine patients relative to healthy controls with a distinct involvement of cerebellar regions, using both parcellations. Specifically, the node degree of the posterior lobe of the cerebellum was greater in patients than in controls and patients presented a higher number of streamlines within the anterior limb of the internal capsule. Moreover, the connectomes of patients exhibited greater global efficiency and shorter characteristic path length, which correlated with the age onset of migraine. Conclusions A distinctive pattern of heightened structural connectivity and enhanced global efficiency in migraine patients compared to controls was identified, which distinctively involves the cerebellum. These findings provide evidence for increased integration within structural brain networks in migraine and underscore the significance of the cerebellum in migraine pathophysiology.

Objective: To examine the clinical effects of combining motor imagery-based neurofeedback training with bilateral repetitive transcranial magnetic stimulation for upper limb motor function in subacute and chronic stroke. Design: Clinical trial following an AB/BA crossover design with counterbalanced assignment. Subjects: Twenty individuals with subacute (n = 4) or chronic stroke (n = 16). Methods: Ten consecutive sessions of bilateral repetitive transcranial magnetic stimulation alone (therapy A) were compared vs a combination of10 consecutive sessions of bilateral repetitive transcranial magnetic stimulation with 12 non-consecutive sessions of motor imagery-based neurofeedback training (therapy B). Patients received both therapies (1-month washout period), in sequence AB or BA. Participants were assessed before and after each therapy and at 15-days follow-up, using the Fugl-Meyer Assessment-upper limb, hand-grip strength, and the Nottingham Sensory Assessment as primary outcome measures. Results: Both therapies resulted in improved functionality and sensory function. Therapy B consistently exhibited superior effects compared with therapy A, according to Fugl-Meyer Assessment and tactile and kinaesthetic sensory function across multiple time-points, irrespective of treatment sequence. No statistically significant differences between therapies were found for hand-grip strength. Conclusion: Following subacute and chronic stroke, integrating bilateral repetitive transcranial magnetic stimulation and motor imagery-based neurofeedback training has the potential to enhance functional performance compared with using bilateral repetitive transcranial magnetic stimulation alone in upper limb recovery.

Abstract Immunological memory is critical for immune protection, particularly at epithelial sites, which are under constant risk of pathogen invasions. To counter invading pathogens, CD8+ memory T cells develop at the location of infection: tissue-resident memory T cells (TRM). CD8+ T-cell responses are associated with type-1 infections and type-1 regulatory T cells (TREG) are important for CD8+ T-cell development, however, if CD8+ TRM cells develop under other infection types and require immune type-specific TREG cells is unknown. We used three distinct lung infection models, to show that type-2 helminth infection does not establish CD8+ TRM cells. Intracellular (type-1) and extracellular (type-3) infections do and rely on the recruitment of response type-matching TREG population contributing transforming growth factor-β. Nevertheless, type-1 TREG cells remain the most important population for TRM cell development. Once established, TRM cells maintain their immune type profile. These results may have implications in the development of vaccines inducing CD8+ TRM cells.

In recent decades, many researchers have focused on the development of Conduct Problems from childhood to adolescence. Understanding behavior problems also requires an understanding of well-regulated characteristics. Focusing our assessment on strengths makes it possible, on the one hand, to help children or adolescents with deficits in important areas (e.g., socio-emotional deficits) to develop emotional regulation skills and adapt their responses to different contexts. This study aims to understand the role of self-competence, self-regulation, empathy, and responsibility (strength variables) in the relationship between Callous Unemotional characteristics and Conduct Problems, with a sample of 236 children aged between 3 and 10 years (M = 7.51, SD = 1.63), through mediation analysis. In general, our findings suggest that self-regulation significantly explains the relationship between the callous dimension of the Inventory of Callous–Unemotional Traits and Conduct Problems, pointing out that this strength variable seems to act as a protective factor against the development of behavior problems. No other mediation effects were found, and these results are considered in light of some limitations.

The importance of assessing psychopathic traits in juveniles who have committed sexual offenses has been established in individuals who demonstrate a particularly severe and violent pattern of behavior. Additionally, the assessment of these traits in other juveniles might be relevant considering that higher levels of these traits represent an increased probability of the juvenile committing offenses. This study is a systematic review of the literature about the presence of callous-unemotional (CU) traits in juveniles who have committed sexual and non-sexual offenses, in order to ascertain eventual differences between these groups regarding the presence of CU traits. Studies were obtained from multiple databases, with predefined exclusion and inclusion criteria, according to PRISMA-P guidelines. A total of 18 studies were reviewed and included in the final analysis. The 18 studies used measures of CU traits and reported descriptive categories of CU traits in juveniles who have committed general offenses or juveniles who have committed sexual offenses. Meta-analytic procedures such as pooled means, pooled variances, and pooled standard deviations are presented in this study. The main conclusion obtained is that juveniles who have committed general offenses present higher levels of CU traits compared to juveniles who have committed sexual offenses. Although the review highlights limitations in the literature, the identification of these characteristics in different types of juveniles who have committed offenses is important to shed light on the phenomenon and develop interventions better suited to their characteristics. Recommendations for future research are also presented.

The simultaneous acquisition of electroencephalography and functional magnetic resonance imaging (EEG-fMRI) allows the complementary study of the brain's electrophysiology and hemodynamics with high temporal and spatial resolution. One application with great potential is neurofeedback training of targeted brain activity, based on the real-time analysis of the EEG and/or fMRI signals. This depends on the ability to reduce in real time the severe artifacts affecting the EEG signal acquired with fMRI, mainly the gradient and pulse artifacts. A few methods have been proposed for this purpose, but they are either slow, hardware-dependent, publicly unavailable, or proprietary software. Here, we present a fully open-source and publicly available tool for real-time EEG artifact reduction in simultaneous EEG-fMRI recordings that is fast and applicable to any hardware. Our tool is integrated in the Python toolbox NeuXus for real-time EEG processing and adapts to a real-time scenario well-established artifact average subtraction methods combined with a long short-term memory network for R peak detection. We benchmarked NeuXus on three different datasets, in terms of artifact power reduction and background signal preservation in resting state, alpha-band power reactivity to eyes closure, and event-related desynchronization during motor imagery. We showed that NeuXus performed at least as well as the only available real-time tool for conventional hardware setups (BrainVision's RecView) and a well-established offline tool (EEGLAB's FMRIB plugin). We also demonstrated NeuXus’ real-time ability by reporting execution times under 250 ms. In conclusion, we present and validate the first fully open-source and hardware-independent solution for real-time artifact reduction in simultaneous EEG-fMRI studies.

Violence in adolescent intimate relationships poses a real threat to adolescent well-being and is a risk factor for recurrent violent patterns in adult marital relationships. The present study aimed to understand the relationship between different dimensions of childhood trauma and dating violence perpetration and the mediating role of temperament. The sample was composed of 3497 adolescents (n = 1549 boys, n = 1948 girls; M = 1.56, SD = 0.497) aged between 10 and 22 years (M = 15.15, SD = 1.83). Instruments used in this study included the Social Desirability Scale, the Childhood Trauma Questionnaire (CTQ), and the Early Adolescent Temperament Questionnaire (EATQ). The results show that temperament plays a mediating role in the relationship between dating violence perpetration and emotional, physical, and sexual abuse. Furthermore, the results suggest that adolescents who have been abused in childhood tend to demonstrate more temperamental problems and a greater susceptibility to the perpetration of teen dating violence, but only in the more severe dimensions of childhood trauma.

Oral insulin delivery could change the life of millions of diabetic patients as an effective, safe, easy-to-use, and affordable alternative to insulin injections, known by an inherently thwarted patient compliance. Here, we designed a multistage nanoparticle (NP) system capable of circumventing the biological barriers that lead to poor drug absorption and bioavailability after oral administration. The nanosystem consists of an insulin-loaded porous silicon NP encapsulated into a pH-responsive lignin matrix, and surface-functionalized with the Fc fragment of immunoglobulin G, which acts as a targeting ligand for the neonatal Fc receptor (FcRn). The developed NPs presented small size (211 ± 1 nm) and narrow size distribution. The NPs remained intact in stomach and intestinal pH conditions, releasing the drug exclusively at pH 7.4, which mimics blood circulation. This formulation showed to be highly cytocompatible, and surface plasmon resonance studies demonstrated that FcRn-targeted NPs present higher capacity to interact and being internalized by the Caco-2 cells, which express FcRn, as demonstrated by Western blot. Ultimately, in vitro permeability studies showed that Fc-functionalized NPs induced an increase in the amount of insulin that permeated across a Caco-2/HT29-MTX co-culture model, showing apparent permeability coefficients (Papp) of 2.37 × 10−6 cm/s, over the 1.66 × 10−6 cm/s observed for their non-functionalized counterparts. Overall, these results demonstrate the potential of these NPs for oral delivery of anti-diabetic drugs.

Nanomedicine’s advent has promised to revolutionize different biomedical fields, including oncology. Silver Nanoparticles (AgNPs) showed promising results in different tumor models. Clear cell Renal Cell Carcinoma (ccRCC) is especially challenging due to its late diagnosis, poor prognosis and treatment resistance. Therefore, defining new therapeutic targets and regimens could improve patient management. This study intends to evaluate AgNPs’ effect in ccRCC cells and explore their potential combinatory effect with Everolimus and Radiotherapy. AgNPs were synthesized, and their effect was evaluated regarding their entering pathway, cellular proliferation capacity, ROS production, mitochondrial membrane depolarization, cell cycle analysis and apoptosis assessment. AgNPs were combined with Everolimus or used to sensitize cells to radiotherapy. AgNPs are cytotoxic to 786-O cells, a ccRCC cell line, entering through endocytosis, increasing ROS, depolarizing mitochondrial membrane, and blocking the cell cycle, leading to a reduction of proliferation capacity and apoptosis. Combined with Everolimus, AgNPs reduce cell viability and inhibit proliferation capacity. Moreover, 786-O is intrinsically resistant to radiation, but after AgNPs’ administration, radiation induces cytotoxicity through mitochondrial membrane depolarization and S phase blockage. These results demonstrate AgNPs’ cytotoxic potential against ccRCC and seem promising regarding the combination with Everolimus and sensitization to radiotherapy, which can, in the future, benefit ccRCC patients’ management.

RESUMO Objetivo analisar demandas de mulheres segundo o olhar de equipes multiprofissionais. Métodos estudo qualitativo desenvolvido em Unidade de Saúde da Família de um Distrito Sanitário de Saúde em Salvador, Bahia, Brasil. Realizou-se grupo focal e o material empírico foi analisado pela técnica de Análise de Discurso. Resultados participaram da pesquisa 12 profissionais de saúde com idades entre 27 e 60 anos e atuação de dois a 13 anos na Estratégia Saúde da Família. As profissionais caracterizaram as usuárias com vulnerabilidade social e econômica e as demandas de ordem afetivo-emocional relacionadas a iniquidades de gênero, com sobrecarga do trabalho doméstico e do cuidado à família. A equipe de saúde constitui ponto de apoio e oferece acolhimento e escuta sensível. Conclusão carência afetiva e desgastes pelas funções no âmbito doméstico, familiar e pela relação com o parceiro marcam as demandas de mulheres por cuidado. Profissionais realizam práticas na perspectiva da integralidade, mas há limites institucionais e da equipe em lidar com singularidades. Construir a superação requer abrir caminhos ao empoderamento das mulheres, o que não retrata a realidade problematizada. Implicações para a prática subsidiar profissionais no atendimento ao princípio da integralidade diante de contextos de mulheres em vulnerabilidades sociais e econômicas.

Objetivo: descrever o processo de implantação de Planos de Alta na Clínica Cirúrgica de um hospital de grande porte de Salvador (BA). Método: relato de experiência que descreve aspectos relacionados a elaboração, atualização e implantação de cinco planos de alta hospitalar de enfermagem para pacientes atendidos nessa clínica cirúrgica. Foram direcionados aos seguintes procedimentos cirúrgicos: hernioplastias, colecistectomia, histerectomia, amputação e prostatectomia. Resultados: foram atualizados cinco planos de alta, os quais foram analisados pela coordenadora de enfermagem, médico cirurgião geral e supervisora do componente obrigatório; após sugestões e alterações realizadas, foram encaminhados para a Comissão de Padronização desse Hospital para serem implantados nas Unidades de Clínica Cirúrgica. Conclusão: a experiência possibilitou a afirmação dos instrumentos como um importante material didático para os pacientes sobre as condutas pós-cirúrgicas e para o enfermeiro, que através desse material consegue direcionar as orientações necessárias.

Abstract Background Mining activities have environmental impacts due to sediment movement and contamination of areas and may also pose risks to people’s food security. In Brazil, the majority of coal mining activities are in the south, in the Santa Catarina carboniferous region. In this region, previously mined areas contaminated with heavy metals frequently occur nearby inhabited zones. Mining is part of the daily lives of local communities, and its environmental impacts are visible in the landscape; however, plants with medicinal and food use from these areas can be still consumed. Heavy metals are contaminants that do not have odor, color, or taste, and are therefore difficult to detect. We aimed to verify whether people use plants from contaminated mine areas, and understand which factors can influence the use of these resources, even from areas visibly impacted. Methods We conducted 195 semi-structured interviews with residents from 14 areas nearby abandoned mines in the main municipalities of the Santa Catarina carboniferous region. We asked each interviewee about the length of time they lived in the region, their perception of the quality of the environment, and what plant species were used and for what purpose. We constructed generalized multivariate linear models to verify which variables can affect the group of species mentioned and generalized linear models to verify which variables can affect the total number of citations. We estimated the frequency of citing species collected using the Smith index. Results From all interviewees, 127 (65%) reported collecting plants for medicinal and food use, directly from contaminated mine areas. Long-term residents, as well as those who noticed more environmental changes (positive and negative), cited more plants used and had more detailed knowledge of plant use in their communities. When asked if they were aware of the possible contamination of mined areas, 85% said they knew about it. However, only 10% associated negative health effects with the use of plant species collected in contaminated mined areas. Conclusions Our study demonstrates that people living nearby contaminated areas use and consume locally sourced plants, e.g., people know little about the danger of this contamination in their food and the risk of these contaminants to their health. These results also reveal a lack of information about contamination, as well as a lack of actions that include local communities in contaminated area restoration strategies. This situation poses a risk to the food security of the people living nearby former coal mining areas.

EEG microstate abnormalities have been reported in patients with schizophrenia. Here the authors demonstrate that patients and their siblings show similar microstate abnormalities compared to healthy controls.

ObjectiveTo summarize evidence for use of advanced MRI techniques as monitoring biomarkers in the clinic, and to highlight the latest bench-to-bedside developments.MethodsThe current evidence regarding the potential for monitoring biomarkers was reviewed and individual modalities of metabolism and/or chemical composition imaging discussed. Perfusion, permeability, and microstructure imaging were similarly analyzed in Part 1 of this two-part review article and are valuable reading as background to this article. We appraise the clinic readiness of all the individual modalities and consider methodologies involving machine learning (radiomics) and the combination of MRI approaches (multiparametric imaging).ResultsThe biochemical composition of high-grade gliomas is markedly different from healthy brain tissue. Magnetic resonance spectroscopy allows the simultaneous acquisition of an array of metabolic alterations, with choline-based ratios appearing to be consistently discriminatory in treatment response assessment, although challenges remain despite this being a mature technique. Promising directions relate to ultra-high field strengths, 2-hydroxyglutarate analysis, and the use of non-proton nuclei. Labile protons on endogenous proteins can be selectively targeted with chemical exchange saturation transfer to give high resolution images. The body of evidence for clinical application of amide proton transfer imaging has been building for a decade, but more evidence is required to confirm chemical exchange saturation transfer use as a monitoring biomarker. Multiparametric methodologies, including the incorporation of nuclear medicine techniques, combine probes measuring different tumor properties. Although potentially synergistic, the limitations of each individual modality also can be compounded, particularly in the absence of standardization. Machine learning requires large datasets with high-quality annotation; there is currently low-level evidence for monitoring biomarker clinical application.ConclusionAdvanced MRI techniques show huge promise in treatment response assessment. The clinical readiness analysis highlights that most monitoring biomarkers require standardized international consensus guidelines, with more facilitation regarding technique implementation and reporting in the clinic.

Objetivo: relatar o desenvolvimento de ações educativas, na perspectiva feminista, que articularam sexualidade e movimentação corporal junto a mulheres da comunidade do Centro de Ciências da Saúde da Universidade Federal do Recôncavo da Bahia, contribuindo para o seu autoempoderamento. Método: estudo descritivo, com abordagem qualitativa, tipo relato de experiência, sobre pesquisa-ação, com metodologia educativa ativa e feminista. Participaram oito mulheres nas sete oficinas realizadas em Santo Antônio de Jesus, Bahia, de setembro a novembro de 2019. Utilizou-se, para as atividades de corpo e coleta de dados, técnicas educativas, referenciadas no arcabouço teórico e metodológico da pedagogia feminista e em metodologias ativas. Resultados: houve diferentes reflexões sobre identidade e as várias formas de ser mulher na sociedade atual. Conclusão: as ações educativas realizadas fortaleceram a troca de conhecimentos, o vínculo e a confiança do grupo, além da vivência coletiva do ser feminino. Descritores: Sexualidade. Mulheres. Corpo Humano. Empoderamento. Dança. Saúde.

ABSTRACT Objective: to discuss in the scientific literature the strategies used to prevent hypothermia in newborns undergoing surgical procedures. Methods this is an integrative literature review, with structured search in April and May 2020 in 08 databases, using the descriptors: Hypothermia; Surgical Procedures, Operative; Infant, Newborn; Protocols. Four primary studies were selected and analyzed using three instruments to assess the methodological quality of the Joanna Briggs Institute and content analysis. Results: Among the strategies used, the following stand out: room temperature control; establishment of humidification and quality of air conditioning cleanliness; use of a heated incubator or cradle; use of thermal mattress; use of caps and blanket; heated fluids; temperature monitoring and abdominal organ coverage. Conclusion good hypothermia prevention strategies were identified, despite the small number of publications on this topic; thus, it points out the need for research with strong evidence.

O estudo objetivou identificar os fatores favorecedores e os comprometedores da qualidade do ensino do curso de graduação em Odontologia da Universidade Federal Fluminense. Trata-se de uma pesquisa exploratória realizada em duas etapas distintas, porém com enfoque integrado. As percepções dos professores e dos alunos foram coletadas por meio de questionários. Para a análise dos dados utilizou-se a Análise de Conteúdo, a estatística descritiva e inferencial. Verificou-se que, na visão acadêmica dos segmentos estudados, o principal fator favorecedor foi qualidade do corpo docente e comprometimento. Já em relação aos comprometedores foram apontados como principais: falta de componentes tais como: competência, motivação, assiduidade, comprometimento e comportamento democrático, por parte de alguns professores; falta de integração e interdisciplinaridade entre as disciplinas; programas curriculares tradicionais, antigos e deficientes.

Objetivo: descrever o processo de implantação de Planos de Alta na Clínica Cirúrgica de um hospital de grande porte de Salvador (BA). Método: relato de experiência que descreve aspectos relacionados a elaboração, atualização e implantação de cinco planos de alta hospitalar de enfermagem para pacientes atendidos nessa clínica cirúrgica. Foram direcionados aos seguintes procedimentos cirúrgicos: hernioplastias, colecistectomia, histerectomia, amputação e prostatectomia. Resultados: foram atualizados cinco planos de alta, os quais foram analisados pela coordenadora de enfermagem, médico cirurgião geral e supervisora do componente obrigatório; após sugestões e alterações realizadas, foram encaminhados para a Comissão de Padronização desse Hospital para serem implantados nas Unidades de Clínica Cirúrgica. Conclusão: a experiência possibilitou a afirmação dos instrumentos como um importante material didático para os pacientes sobre as condutas pós-cirúrgicas e para o enfermeiro, que através desse material consegue direcionar as orientações necessárias.

Sexting has been defined as sending, receiving, or forwarding sexually explicit messages, images, or photos to others through digital platforms, and can assume more consensual or more abusive and violent forms. This study aims to explore the prevalence of abusive sexting in Portuguese adolescents and the psychological characteristics of sexting abusers in terms of emotional and behavioral problems, potential markers of psychopathy, childhood trauma and maltreatment, and different forms of aggression. A cross-sectional study was conducted with 4,281 participants, aged 12–20 years (2,264 girls and 2,017 boys), of whom 204 (4.8%) engaged in abusive sexting behaviors and 182 (4.3%) self-identified as being a non-consensual sexting victim. Abusive sexting was more common among boys and middle adolescents, and abusive sexting victims were more likely to be children of single-parent families. Engaging in abusive sexting and being a victim of abusive sexting were also related to behavioral and emotional problems, callousness, experiences of neglect and abuse in childhood, and various forms of aggression. Implications for future research and intervention are discussed.

Cerebrovascular reactivity (CVR) is defined as the ability of vessels to alter their caliber in response to vasoactive factors, by means of dilating or constricting, in order to increase or decrease regional cerebral blood flow (CBF). Importantly, CVR may provide a sensitive biomarker for pathologies where vasculature is compromised. Furthermore, the spatiotemporal dynamics of CVR observed in healthy subjects, reflecting regional differences in cerebral vascular tone and response, may also be important in functional MRI studies based on neurovascular coupling mechanisms. Assessment of CVR is usually based on the use of a vasoactive stimulus combined with a CBF measurement technique. Although transcranial Doppler ultrasound has been frequently used to obtain global flow velocity measurements, MRI techniques are being increasingly employed for obtaining CBF maps. For the vasoactive stimulus, vasodilatory hypercapnia is usually induced through the manipulation of respiratory gases, including the inhalation of increased concentrations of carbon dioxide. However, most of these methods require an additional apparatus and complex setups, which not only may not be well-tolerated by some populations but are also not widely available. For these reasons, strategies based on voluntary breathing fluctuations without the need for external gas challenges have been proposed. These include the task-based methodologies of breath holding and paced deep breathing, as well as a new generation of methods based on spontaneous breathing fluctuations during resting-state. Despite the multitude of alternatives to gas challenges, existing literature lacks definitive conclusions regarding the best practices for the vasoactive modulation and associated analysis protocols. In this work, we perform an extensive review of CVR mapping techniques based on MRI and CO2 variations without gas challenges, focusing on the methodological aspects of the breathing protocols and corresponding data analysis. Finally, we outline a set of practical guidelines based on generally accepted practices and available data, extending previous reports and encouraging the wider application of CVR mapping methodologies in both clinical and academic MRI settings.

O artigo apresenta os principais enfoques adotados no tratamento das questões acerca da pobreza e das desigualdades sociais bem como das políticas habitacionais no Brasil. A partir desse cenário apresentado pretende-se destacar elementos considerados de importante potencial analítico que vêm sendo pouco explorados, apontando, nesse sentido, algumas lacunas identificadas nos estudos sobre a questão da habitação. Ao dialogar e problematizar a literatura disponível sobre o tratamento da pobreza, das desigualdades e suas expressões na questão habitacional, percebe-se a segregação espacial não como consequência, mas como elemento constitutivo das ações públicas nesse âmbito.

To maximize brain plasticity after stroke, a plethora of rehabilitation strategies have been explored. These include the use of intensive motor training, motor-imagery (MI), and action-observation (AO). Growing evidence of the positive impact of virtual reality (VR) techniques on recovery following stroke has been shown. However, most VR tools are designed to exploit active movement, and hence patients with low level of motor control cannot fully benefit from them. Consequently, the idea of directly training the central nervous system has been promoted by utilizing MI with electroencephalography (EEG)-based brain-computer interfaces (BCIs). To date, detailed information on which VR strategies lead to successful functional recovery is still largely missing and very little is known on how to optimally integrate EEG-based BCIs and VR paradigms for stroke rehabilitation. The purpose of this study was to examine the efficacy of an EEG-based BCI-VR system using a MI paradigm for post-stroke upper limb rehabilitation on functional assessments, and related changes in MI ability and brain imaging. To achieve this, a 60 years old male chronic stroke patient was recruited. The patient underwent a 3-week intervention in a clinical environment, resulting in 10 BCI-VR training sessions. The patient was assessed before and after intervention, as well as on a one-month follow-up, in terms of clinical scales and brain imaging using functional MRI (fMRI). Consistent with prior research, we found important improvements in upper extremity scores (Fugl-Meyer) and identified increases in brain activation measured by fMRI that suggest neuroplastic changes in brain motor networks. This study expands on the current body of evidence, as more data are needed on the effect of this type of interventions not only on functional improvement but also on the effect of the intervention on plasticity through brain imaging.

Eimeria vermiformis is a tissue specific, intracellular protozoan that infects the murine small intestinal epithelia, which has been widely used as a coccidian model to study mucosal immunology. This mouse infection model is valuable to investigate the mechanisms of host protection against primary and secondary infection in the small intestine. Here, we describe the generation of an E. vermiformis stock solution, preparation of sporulated E. vermiformis to infect mice and determination of oocysts burden. This protocol should help to establish a highly reproducible natural infection challenge model to study immunity in the small intestine. The information obtained from using this mouse model can reveal fundamental mechanisms of interaction between the pathogen and the immune response, e.g., provided by intraepithelial lymphocytes (IEL) at the basolateral site of epithelial cells but also a variety of other immune cell populations present in the gut.

Abstract Purpose To verify sociodemographic factors associated with the occurrence of abortion in women of reproductive age, in areas covered by the Family Health Strategy (FHS), a program from the Brazilian Ministry of Health. Methods A cross-sectional study using household surveys of 350 women aged 15 to 49. The report of abortion was a variable indicator, and sociodemographic aspects were covariables. Prevalence ratio (PR) and respective 95% confidence intervals were used to estimate the magnitude of the associations. Results There were associations among age, civil status, race/color, and religion; an increase in the prevalence of lower levels of education, age less than 20, and student status were protective factors. Conclusion The association between sociodemographic characteristics and the report of abortion is attributed to the fact that there is a lack at the FHS in the availability of fundamental healthcare services for young women; these findings call for action to guarantee the access to information about contraceptives and guidance to decrease the risk of unplanned pregnancies and abortions.

Objectives: Childhood absence epilepsy (CAE) is a syndrome with well-defined electroclinical features but unknown pathological basis. An increased thalamic tonic GABA inhibition has recently been discovered on animal models (Cope et al., 2009), but its relevance for human CAE is unproven. Methods: We studied an 11-year-old boy, presenting the typical clinical features of CAE, but spike–wave discharges (SWD) restricted to one hemisphere. Results: High-resolution EEG failed to demonstrate independent contralateral hemisphere epileptic activity. Consistently, simultaneous EEG–fMRI revealed the typical thalamic BOLD activation, associated with caudate and default mode network deactivation, but restricted to the hemisphere with SWD. Cortical BOLD activations were localized on the ipsilateral pars transverse. Magnetic resonance spectroscopy, using MEGA-PRESS, showed that the GABA/creatine ratio was 2.6 times higher in the hemisphere with SWD than in the unaffected one, reflecting a higher GABA concentration. Similar comparisons for the patient's occipital cortex and thalamus of a healthy volunteer yielded asymmetries below 25%. Significance: In a clinical case of CAE with EEG and fMRI-BOLD manifestations restricted to one hemisphere, we found an associated increase in thalamic GABA concentration consistent with a role for this abnormality in human CAE.

The simultaneous acquisition of electroencephalography (EEG) with functional magnetic resonance imaging (fMRI) is a very promising non-invasive technique for the study of human brain function. Despite continuous improvements, it remains a challenging technique, and a standard methodology for data analysis is yet to be established. Here we review the methodologies that are currently available to address the challenges at each step of the data analysis pipeline. We start by surveying methods for pre-processing both EEG and fMRI data. On the EEG side, we focus on the correction for several MR-induced artifacts, particularly the gradient and pulse artifacts, as well as other sources of EEG artifacts. On the fMRI side, we consider image artifacts induced by the presence of EEG hardware inside the MR scanner, and the contamination of the fMRI signal by physiological noise of non-neuronal origin, including a review of several approaches to model and remove it. We then provide an overview of the approaches specifically employed for the integration of EEG and fMRI when using EEG to predict the blood oxygenation level dependent (BOLD) fMRI signal, the so-called EEG-informed fMRI integration strategy, the most commonly used strategy in EEG-fMRI research. Finally, we systematically review methods used for the extraction of EEG features reflecting neuronal phenomena of interest.

The study aimed to analyze the available evidence in the literature on nursing care in the hospital post-cardiac surgery. Data were collected from electronic databases LILACS, SciELO, MEDLINE, via DeCS thoracic surgery, hospital, nursing care, in the period 2001 to 2011. Ten articles were selected that showed the need to develop a plan of nursing discharge focusing on prevention of complications and coping with physical limitations resulting from heart surgery. Thus, the discharge should be considered from the time of admission, with carefully planned actions involving patient and family.

A dislipidemia é um dos grandes fatores de risco associados a doenças cardiovasculares. Poucos são os dados relacionados ao impacto da cardiopatia congênita na prevalência da dislipidemia na população pediátrica. O objetivo deste estudo foi avaliar o perfil lipídico de crianças portadoras de cardiopatia congênita seguidas em um centro de referência. Foram incluídos 52 pacientes pediátricos que tiveram perfil lipídico, metabólico e clínico determinados entre janeiro de 2011 e julho de 2012. Com média de idade de 10,4 ± 2,8 anos e predominância do sexo masculino (1,38:1), nossa população apresentou 53,8% de pacientes com aumento no colesterol total e 13,4% (IC 95% de 6,6-25,2%) de crianças que também apresentavam LDL > 130 mg/dL, caracterizando dislipidemia. Dos pacientes com dislipidemia, só doisforam classificados como obesos. Concluímos que a presença de cardiopatia congênita não confere risco aumentado associado à presença de dislipidemia, devendo o rastreamento nessa população seguir as mesmas diretrizes da população pediátrica normal, as quaistambém independem do estado nutricional da criança.

O estudo objetivou analisar a inclusão dos conceitos de saúde sexual e reprodutiva nos currículos da Escola de Enfermagem da Universidade Federal da Bahia, de 1972 a 2006. Utilizou a abordagem qualitativa, a pesquisa documental e a entrevista semiestruturada com docentes em regime de dedicação exclusiva. Os resultados revelaram que, inicialmente, as disciplinas eram exclusivamente voltadas aos aspectos biológicos da saúde da mulher, enfocando apenas a condição materna. Os conceitos de saúde sexual e reprodutiva, na perspectiva feminista, foram introduzidos posteriormente, para atender demandas de políticas de formação de mão-de-obra na área de saúde, representando também uma ampliação na compreensão da condição social da mulher. Conclui-se que ocorreu uma evolução nos currículos com a introdução desses conceitos, principalmente a partir da década de 1990.

Este trabalho objetivou analisar o uso das propostas feministas no processo de ensino aprendizagem de disciplinas relacionadas saúde sexual e reprodutiva na graduação em enfermagem. Metodologicamente, trata-se de um estudo qualitativo, cuja técnica de produção de dados foi a a entrevista semi-estruturada. O critério de inclusão utilizado foi: instituições pública e privada de ensino superior de Salvador-Bahia, que já tivessem formado ao menos uma turma do curso de graduação em enfermagem até 2008 e que houvessem docentes com mestrado em enfermagem pelo programa de Pós Graduação em Enfermagem da Universidade Federal da Bahia (PPGEUFBA), na linha de Mulher, Saúde e Gênero e lecionassem/lecionaram a(s) disciplina(s) relacionada à temática saúde sexual e reprodutiva. Os resultados apontaram que os conceitos de saúde sexual e reprodutiva estão de certa forma, inseridos na graduação em enfermagem, porém ainda se concentram na disciplina de saúde da mulher, apesar da tentativa das docentes de promover a transversalidade desses conceitos para outras disciplinas. Considera-se que esta pesquisa confirma a necessidade da formação de profissionais com a noção de integralidade na atenção já que as questões relativas a saúde sexual e reprodutiva não devem ser restritas a componentes curriculares relacionados a saúde da mulher.

Relato de três casos de quilotórax e um caso de ascite quilosa em crianças em pós-operatório de cirurgia cardíaca, que evoluíram sem resposta ao tratamento clínico habitual, baseado em jejum e nutrição parenteral prolongada. Tratamento com octreotide na dose inicial de 1,0 mcg/kg/h foi escolhido, com aumento progressivo de 1,0 mcg/kg/h/dia até a dose máxima de 4,0 mcg/kg/h. Todos os casos tiveram resposta favorável, com redução progressiva do débito do dreno, até resolução do quadro, sem efeito colateral significativo.

The importance of music in our daily life has given rise to an increased number of studies addressing the brain regions involved in its appreciation. Some of these studies controlled only for the familiarity of the stimuli, while others relied on pleasantness ratings, and others still on musical preferences. With a listening test and a functional magnetic resonance imaging (fMRI) experiment, we wished to clarify the role of familiarity in the brain correlates of music appreciation by controlling, in the same study, for both familiarity and musical preferences. First, we conducted a listening test, in which participants rated the familiarity and liking of song excerpts from the pop/rock repertoire, allowing us to select a personalized set of stimuli per subject. Then, we used a passive listening paradigm in fMRI to study music appreciation in a naturalistic condition with increased ecological value. Brain activation data revealed that broad emotion-related limbic and paralimbic regions as well as the reward circuitry were significantly more active for familiar relative to unfamiliar music. Smaller regions in the cingulate cortex and frontal lobe, including the motor cortex and Broca's area, were found to be more active in response to liked music when compared to disliked one. Hence, familiarity seems to be a crucial factor in making the listeners emotionally engaged with music, as revealed by fMRI data.

A política de reogarnização do Sistema de Saúde no Brasil utiliza a estratégia de atendimento domiciliar, tendo a atenção primária como base de sua ação, revalorizando o uso de práticas de saúde no domicílio. Este espaço, histórico e culturalmente atribuído às mulheres, influencia e é influenciado por diferentes práticas sociais. Este estudo, que é um corte da dissertação de mestrado "Práticas de Saúde da Mulher no Espaço Domiciliar: análise a partir de Agentes Comunitários de Saúde (ACS) do Distrito Sanitária Barra/Rio Vermelho, em Salvador. Trata-se de um estudo qualitativo, do tipo descritivo que utiliza como referencial teórico filosófico o Materialismo Histórico Dialético. Para a coleta de dados, empregou-se a observação participante e a entrevista semi-estruturada gravada. O método de análise de conteúdo. A visita domiciliar apresenta-se como instrumento de trabalho revalorizado. A articulação dos ACS com os serviços apresenta-se com deficiências, considerando-se o atendimento à demanda espontânea. O trabalho dos agentes reproduz características do Modelo Médico Assistencial. Da mesma forma, as práticas de saúde, ainda que voltadas para a promoção/prevenção de problemas de saúde, não são suficientes para enfrentar os conflitos e as necessidades existentes no âmbito domiciliar. O despreparo dos Agentes para lidar com problemas complexos reproduz desigualdades de gênero, na medida em que reforçam o papel da mulher como única cuidadora, bem como estereótipos sexistas.

ABSTRACT Objective: To identify in the scientific literature the clinical overview of the pediatric population that tested positive for SARS-CoV-2 and care recommendations and recommendations among children who tested positive for SARS-CoV-2 in the scientific literature. Method: Rapid review based on the guidelines of the Joana Briggs Institute: elaboration of the research question, structured search of the literature in April 2020, in nine databases, selection and critical analysis of the eighteen primary studies (using two instruments to assess methodological quality), elaboration of the synthesis, incorporation of suggestions and dissemination. Result: The most frequent clinical overview was respiratory, gastrointestinal symptoms and fever. The images showed irregular frosted glass opacification. It is recommended to screen the pediatric population and family members who show signs and symptoms and to adopt isolation for more than fourteen days. Conclusion: The clinical overview in pediatric population is varied, not exclusively with respiratory symptoms, and a significant number of asymptomatic patients. The importance of new investigations is highlighted, such as randomized clinical trial or cohort studies, identifying their participation in the transmission of COVID-19.