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281 results on '"Panda, Amulya K."'

30. Electrochemical Detection of Vibrio cholerae by Amine Functionalized Biocompatible Gadolinium Oxide Nanoparticles.

Author

Kumar, Ashutosh, Sarkar, Tamal, Kumar, Robin, Panda, Amulya K., and Solanki, Pratima R.

Subjects
VIBRIO cholerae, CHOLERA toxin, INDIUM tin oxide, TRANSMISSION electron microscopy, GADOLINIUM, AMINES
Abstract

Herein, we report the biocompatible amine-functionalized gadolinium oxide nanoparticles (Gd2O3 NPs) for the possibility of electrochemical detection of Vibrio cholerae (Vc) cells. The microwave irradiation process is applied to synthesize Gd2O3 NPs. The amine (NH2) functionalization is carried out via overnight stirring with 3(Aminopropyl)triethoxysilane (APTES) at 55 °C. The size of NPs amine functionalized APETS@Gd2O3 NPs are determined by transmission electron microscopy (TEM). APETS@Gd2O3 NPs are further electrophoretically deposited onto indium tin oxide (ITO) coated glass substrate to obtain working electrode surface. The monoclonal antibodies (anti-CT) specific to cholera toxin associated to Vc cells are covalently immobilized onto the above electrodes using EDC-NHS chemistry and further BSA is added to obtain the BSA/anti-CT/APETS@Gd2O3/ITO immunoelectrode. Further, this immunoelectrode shows the response for cells in CFU range from 3.125 × 106 to 30 × 106 and is very selective with sensitivity and LOD 5.07 mA CFUs mL cm−2 and 0.9375 × 106 CFU respectively. To establish a future potential for APTES@Gd2O3 NPs in field of biomedical applications and cytosensing, the effect of APTES@Gd2O3 NPs on mammalian cells is also observed using in vitro cytotoxicity assay and cell cycle analysis. [ABSTRACT FROM AUTHOR]

Published
2023
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45. Sustainable Agriculture Reviews 44

Author

Saneja, Ankit, Panda, Amulya K, Lichtfouse, Eric, Centre européen de recherche et d'enseignement des géosciences de l'environnement (CEREGE), Aix Marseille Université (AMU)-Institut national des sciences de l'Univers (INSU - CNRS)-Collège de France (CdF (institution))-Institut de Recherche pour le Développement (IRD)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA), Ankit Saneja, Amulya K. Panda, Eric Lichtfouse, and Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Collège de France (CdF (institution))-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)

Subjects
nanoarchitectonic, lipid, [SDU.STU.GC]Sciences of the Universe [physics]/Earth Sciences/Geochemistry, drug delivery, nanoemulsion, [SDV.SP]Life Sciences [q-bio]/Pharmaceutical sciences, [SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics
Abstract

International audience; This book covers nanotechnology based approaches for improving the therapeutic efficacy of natural products. It critically explores lipid nanoarchitectonics, inorganic particles and nanoemulsion based tools for delivering them. With its chapters from eminent experts working in this discipline, it is ideal for researchers and professionals working in the area.

Published
2020
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48. Contraceptive efficacy of recombinant porcine zona proteins and fusion protein encompassing canine ZP3 fragment and GnRH in female beagle dogs.

Author

Gupta, Satish K., Toor, Sagarika, Minhas, Vidisha, Chaudhary, Piyush, Raman, Muthusamy, Anoop, S., and Panda, Amulya K.

Subjects
BEAGLE (Dog breed), FEMALE dogs, CHIMERIC proteins, CONTRACEPTION, RECOMBINANT proteins
Abstract

Problem: To manage population of dogs (Canis familiaris), the efficacy of recombinant proteins‐based contraceptive vaccines to inhibit fertility has been evaluated in female beagle dogs. Method of study: Female beagle dogs (n = 4) were immunized with physical mixture of Escherichia coli‐expressed recombinant porcine ZP3 with promiscuous T cell epitope of tetanus toxoid (TT‐KK‐pZP3) and porcine ZP4 with promiscuous T cell epitope of bovine RNase (bRNase‐KK‐pZP4), or with a fusion protein encompassing dog ZP3 fragment and two copies of GnRH with appropriate promiscuous T cell epitopes (dZP3‐GnRH2); control animals received only alum, the adjuvant. The immunized animals were followed‐up for antibody titres by ELISA as well as for fertility status subsequent to mating with male dogs. Results: Active immunization of female dogs following a three injections schedule at 4‐week intervals with a physical mixture of TT‐KK‐pZP3 + bRNase‐KK‐pZP4 as well as dZP3‐GnRH2, led to generation of significant antibody titres against respective recombinant proteins. Active immunization with dZP3‐GnRH2 also led to generation of antibodies reactive with both dZP3 and GnRH. A booster dose on day 383 led to an increase in antibody titres and circulating antibodies against respective recombinant proteins could be observed on day 528. Antibodies in immune serum samples from dogs immunized with TT‐KK‐pZP3 + bRNase‐KK‐pZP4 or dZP3‐GnRH2 reacted with native canine ZP as assessed by an indirect immunofluorescence assay. Mating studies revealed a reduced number of pregnancies as well as a significant reduction in the number of pups born in the female dogs immunized with dZP3‐GnRH2 as compared to the adjuvanted control. Curtailment of pregnancy in dZP3‐GnRH2 immunized group was associated with antibody titres against dZP3‐GnRH2. However, immunization with recombinant TT‐KK‐pZP3 + bRNase‐KK‐pZP4 did not significantly decrease the number of pups born as compared to the adjuvanted control. Conclusion: These studies revealed the potential of recombinant dZP3‐GnRH2‐based contraceptive vaccine to curtail fertility in female dogs. Large scale studies to establish the efficacy and safety of this recombinant protein for the management of community dog population are thus warranted. [ABSTRACT FROM AUTHOR]

Published
2022
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